FvH4_5g31491

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001194
Physical Location & Seq
Reverse (-)
22463567 .. 22467521
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31491.t1

Sequence Viewer

Length: 1524 bp
ATGTCGAAAACTCCGGCTAGCCCAAATTTTGTCAATCTCGGTGATGAACCCACAATGAAGGAGCCTTTGAATCTGTTGCCTCTTCAAGCTGAGGTGCCTTTCCCTGGCGGCCAATCTCTGTTGCTCACTGCTACCCCTGGTGGCCCCTCCATAGCTGACAAGCTTCAAGCTCTGATGGACCCAGTCCCTGCTGGGGCGCTGGGTAAACGACCCCACTCTCCCTCTGGTGAAATTCTGGTCAAGATCGCCAAGCCATCTGTCTCCAGTGGTGGTGGACCATCCTCAGCAGTTCCCTCTTCTCCAGCCTCTCCGGTTAGGTCTCCAATTAGGACTCCCTCTCCTTCTTCTGAGGGTGTATGGACCGAGTTGCCTGATCCAGTTCCTGAGCCCAAATCCAAGCGCTGCATGTCCAAGCGTCGAGCGTCGAGGCCTGCCTCTCCTCCTAGGACCGAAGGGCCCTCTGTTCCCCCTATCGCTCCTCTGTCTGGTATGCCTAAGGATCCTTCCTTTCCCATTTCTCCTCCATCCTCTAGCCCTAAGCCCTCAGAACGCTCCAAGCCAGCTCCGGTCTCTCGCAATCTTCATTCTGGGCCTCGAAATGCTGTAGATAGGGGCAAGGCTCCCTTGGCAGAACGTCGGGTCCCTCCTAAGAAAAGGTCTGGTGAGTCTGCTTCTACCAATTTTCCTCCTATGTCTTATTTTGAGTCTGACTCCCTTCGTGATCTTTTCAATACCTTTGTGATGGGTAAGAAAATTGTGTATGAAAGACCCCTAGCTTTAGACTCTTTTCATCCTGAGATTAGGAAACTTCTTCAGCATTGGGGATGGGATCAAGTCTTAGAGTCCTTTCCTAAGGTTAATAAGACTTGGGTTCAGGAGTTTTACACCAACTTTCCCCAACAAGCTCCTAAAAAGGTTAAGATTCAGCGTCAATCCCCTATTGTTGTCCGGGTTCGGGGTAAGAATGTAGATGTCTCTCCTCCTGGGATTCGTGCTAGTCTTTCTCTCCCCGAGACTGCACCGAATGCCATTCAGTCTGTCTTGGAATTCATTGACTCCATTTCTCTTCAAGATCTGGGTGTTGCTCTTTATCAGTCTTCCTTTCCTATTCCTGCCTTGACTACACCCCATCTTTCTAGTCAGTTTATGTCTGAACTTTTCAAGATTTTAGGGTTTGTGGTGCGCTCTTTGCTTACTCCTACCACCAACACCATTCCGGTTGACAAGCTGTCTTTGGCTAAATCTGTTGGTCAATCTGGTGATGTGTATATTGAGCCCCTTGAGTCCACTTTGTTGAAGCAGGAAGTTAGGCATCAGGTTTCCAGTGCAGTGGCTACCTTGACACAGTTGTTTGCTTCTCACCTTTCTAAACTGACCCTGGAGCTAAAGGAGCTGAAAGAGTCCGTAAGGTCGCATCAGGCCTCTGCTATCCCCTCTGCTGGGGCCCCTGTAGCTGATGAGGAATCCTCCTCAGAGTCAGGATCTGAGGAGACATCTTCCAGTGATGGAAAGGAAGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

508

Amino Acids

54.4

Weight (kDa)

9.39

Isoelectric Point (pI)

74.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_32 PF20167 274 - 404 8.5e-10 Putative plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AciI CCGC 1 cut(s) 108
AclWI GGATC 5 cut(s) 368, 494, 507, 837, 1489
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 3 cut(s) 25, 231, 1046
AcuI CTGAAG 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 401
AfiI CCNNNNNNNGG 6 cut(s) 104, 193, 485, 955, 1439, 1440
AgsI TTSAA 7 cut(s) 70, 86, 167, 730, 1070, 1162, 1297
AhdI GACNNNNNGTC 1 cut(s) 1228
AjnI CCWGG 4 cut(s) 103, 136, 982, 1377
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
Alw26I GTCTC 6 cut(s) 265, 324, 574, 979, 1007, 1484
AlwI GGATC 5 cut(s) 368, 494, 507, 837, 1489
AlwNI CAGNNNCTG 3 cut(s) 188, 383, 1484
Ama87I CYCGRG 1 cut(s) 1010
Aor51HI AGCGCT 1 cut(s) 401
AoxI GGCC 7 cut(s) 109, 142, 428, 455, 590, 1419, 1443
ApaI GGGCCC 2 cut(s) 459, 1447
ApeKI GCWGC 1 cut(s) 402
ApoI RAATTY 3 cut(s) 25, 231, 1046
AspA2I CCTAGG 1 cut(s) 443
AspLEI GCGC 3 cut(s) 199, 402, 1185
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 5 cut(s) 53, 239, 674, 1271, 1352
AsuNHI GCTAGC 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 1010
AvaII GGWCC 5 cut(s) 178, 275, 360, 447, 640
AvrII CCTAGG 1 cut(s) 443
AxyI CCTNAGG 2 cut(s) 495, 852
BaeGI GKGCMC 2 cut(s) 459, 1447
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 4 cut(s) 390, 459, 1278, 1447
BbsI GAAGAC 1 cut(s) 1089
BbvCI CCTCAGC 2 cut(s) 90, 283
BbvI GCAGC 1 cut(s) 389
BccI CCATC 8 cut(s) 169, 262, 286, 532, 736, 819, 1137, 1499
BciT130I CCWGG 4 cut(s) 105, 138, 984, 1379
BcnI CCSGG 1 cut(s) 950
BcoDI GTCTC 6 cut(s) 265, 324, 574, 979, 1007, 1484
BfaI CTAG 6 cut(s) 18, 444, 531, 773, 996, 1137
BfmI CTRYAG 2 cut(s) 603, 1449
BfoI RGCGCY 2 cut(s) 200, 403
BglII AGATCT 1 cut(s) 1072
BisI GCNGC 2 cut(s) 109, 403
BlnI CCTAGG 1 cut(s) 443
BlsI GCNGC 2 cut(s) 110, 404
Bme1390I CCNGG 5 cut(s) 105, 138, 950, 984, 1379
Bme18I GGWCC 5 cut(s) 178, 275, 360, 447, 640
BmeRI GACNNNNNGTC 1 cut(s) 1228
BmeT110I CYCGRG 1 cut(s) 1010
BmrFI CCNGG 5 cut(s) 105, 138, 950, 984, 1379
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 1321, 1423
BmtI GCTAGC 1 cut(s) 21
BmuI ACTGGG 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 1089
BplI GAGNNNNNCTC 4 cut(s) 695, 727, 1451, 1483
BpmI CTGGAG 3 cut(s) 247, 285, 1400
Bpu10I CCTNAGC 4 cut(s) 90, 283, 384, 537
BpuEI CTTGAG 1 cut(s) 1301
BpuMI CCSGG 1 cut(s) 950
BsaI GGTCTC 2 cut(s) 324, 574
BsaJI CCNNGG 6 cut(s) 103, 136, 443, 624, 983, 1377
BsaWI WCCGGW 3 cut(s) 310, 565, 1216
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 193, 485, 955, 1439, 1440
Bse1I ACTGG 5 cut(s) 182, 264, 377, 1323, 1500
Bse21I CCTNAGG 2 cut(s) 495, 852
BseBI CCWGG 4 cut(s) 105, 138, 984, 1379
BseDI CCNNGG 6 cut(s) 103, 136, 443, 624, 983, 1377
BseGI GGATG 4 cut(s) 278, 524, 790, 830
BseLI CCNNNNNNNGG 6 cut(s) 104, 193, 485, 955, 1439, 1440
BseMII CTCAG 8 cut(s) 81, 297, 339, 375, 558, 786, 1476, 1485
BseNI ACTGG 5 cut(s) 182, 264, 377, 1323, 1500
BseRI GAGGAG 6 cut(s) 429, 468, 510, 969, 1459, 1502
BseSI GKGCMC 2 cut(s) 459, 1447
BseXI GCAGC 1 cut(s) 389
BseYI CCCAGC 3 cut(s) 191, 199, 1439
BsgI GTGCAG 2 cut(s) 1002, 1347
BshFI GGCC 7 cut(s) 111, 144, 430, 457, 592, 1421, 1445
BshNI GGYRCC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 1010
BsiSI CCGG 5 cut(s) 14, 311, 566, 949, 1217
BslFI GGGAC 2 cut(s) 170, 626
BslI CCNNNNNNNGG 6 cut(s) 104, 193, 485, 955, 1439, 1440
BsmAI GTCTC 6 cut(s) 265, 324, 574, 979, 1007, 1484
BsmFI GGGAC 2 cut(s) 170, 626
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 7 cut(s) 111, 144, 430, 457, 592, 1421, 1445
Bso31I GGTCTC 2 cut(s) 324, 574
BsoBI CYCGRG 1 cut(s) 1010
Bsp120I GGGCCC 2 cut(s) 455, 1443
Bsp1286I GDGCHC 4 cut(s) 390, 459, 1278, 1447
Bsp143I GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
BspACI CCGC 1 cut(s) 108
BspANI GGCC 7 cut(s) 111, 144, 430, 457, 592, 1421, 1445
BspCNI CTCAG 8 cut(s) 82, 296, 340, 376, 557, 787, 1477, 1484
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 5 cut(s) 368, 494, 507, 837, 1489
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 2 cut(s) 324, 574
BsrI ACTGG 5 cut(s) 182, 264, 377, 1323, 1500
BssECI CCNNGG 6 cut(s) 103, 136, 443, 624, 983, 1377
BssMI GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
BssT1I CCWWGG 2 cut(s) 443, 624
Bst2UI CCWGG 4 cut(s) 105, 138, 984, 1379
Bst4CI ACNGT 1 cut(s) 1347
Bst6I CTCTTC 3 cut(s) 87, 301, 1071
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstC8I GCNNGC 3 cut(s) 19, 432, 561
BstF5I GGATG 4 cut(s) 278, 524, 790, 830
BstH2I RGCGCY 2 cut(s) 200, 403
BstHHI GCGC 3 cut(s) 199, 402, 1185
BstKTI GATC 7 cut(s) 246, 376, 502, 724, 832, 1075, 1484
BstMAI GTCTC 6 cut(s) 265, 324, 574, 979, 1007, 1484
BstMBI GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
BstMWI GCNNNNNNNGC 5 cut(s) 626, 1025, 1189, 1390, 1451
BstNI CCWGG 4 cut(s) 105, 138, 984, 1379
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 5 cut(s) 103, 136, 948, 982, 1377
BstSFI CTRYAG 2 cut(s) 603, 1449
BstSLI GKGCMC 2 cut(s) 459, 1447
BstV1I GCAGC 1 cut(s) 389
BstV2I GAAGAC 1 cut(s) 1089
BstX2I RGATCY 3 cut(s) 499, 1072, 1481
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 3 cut(s) 499, 1072, 1481
Bsu36I CCTNAGG 2 cut(s) 495, 852
BsuRI GGCC 7 cut(s) 111, 144, 430, 457, 592, 1421, 1445
BtsCI GGATG 4 cut(s) 278, 524, 790, 830
BtsI GCAGTG 2 cut(s) 126, 1335
BtsIMutI CAGTG 5 cut(s) 126, 271, 1330, 1335, 1507
Cac8I GCNNGC 3 cut(s) 19, 432, 561
CaiI CAGNNNCTG 3 cut(s) 188, 383, 1484
CfoI GCGC 3 cut(s) 199, 402, 1185
CseI GACGC 3 cut(s) 404, 411, 917
CviAII CATG 1 cut(s) 406
DpnI GATC 7 cut(s) 245, 375, 501, 723, 831, 1074, 1483
DpnII GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
DriI GACNNNNNGTC 1 cut(s) 1228
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 3 cut(s) 87, 301, 1071
Eam1105I GACNNNNNGTC 1 cut(s) 1228
EarI CTCTTC 3 cut(s) 87, 301, 1071
Eco130I CCWWGG 2 cut(s) 443, 624
Eco147I AGGCCT 2 cut(s) 430, 1421
Eco24I GRGCYC 4 cut(s) 390, 459, 1278, 1447
Eco31I GGTCTC 2 cut(s) 324, 574
Eco47I GGWCC 5 cut(s) 178, 275, 360, 447, 640
Eco47III AGCGCT 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 797
Eco81I CCTNAGG 2 cut(s) 495, 852
Eco88I CYCGRG 1 cut(s) 1010
EcoO109I RGGNCCY 5 cut(s) 455, 456, 640, 1443, 1444
EcoRI GAATTC 1 cut(s) 1046
EcoRII CCWGG 4 cut(s) 103, 136, 982, 1377
EcoT14I CCWWGG 2 cut(s) 443, 624
EcoT38I GRGCYC 4 cut(s) 390, 459, 1278, 1447
ErhI CCWWGG 2 cut(s) 443, 624
FaeI CATG 1 cut(s) 409
FaiI YATR 8 cut(s) 152, 358, 407, 491, 692, 762, 1148, 1269
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FaqI GGGAC 2 cut(s) 170, 626
FatI CATG 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 109, 403
FokI GGATG 4 cut(s) 265, 511, 777, 837
FriOI GRGCYC 4 cut(s) 390, 459, 1278, 1447
Fsp4HI GCNGC 2 cut(s) 109, 403
FspBI CTAG 6 cut(s) 18, 444, 531, 773, 996, 1137
GlaI GCGC 3 cut(s) 198, 401, 1184
GluI GCNGC 2 cut(s) 109, 403
GsaI CCCAGC 3 cut(s) 195, 203, 1443
GsuI CTGGAG 3 cut(s) 247, 285, 1400
HaeII RGCGCY 2 cut(s) 200, 403
HaeIII GGCC 7 cut(s) 111, 144, 430, 457, 592, 1421, 1445
HapII CCGG 5 cut(s) 14, 311, 566, 949, 1217
HgaI GACGC 3 cut(s) 404, 411, 917
HhaI GCGC 3 cut(s) 199, 402, 1185
Hin1II CATG 1 cut(s) 409
Hin6I GCGC 3 cut(s) 197, 400, 1183
HinP1I GCGC 3 cut(s) 197, 400, 1183
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 5 cut(s) 14, 311, 566, 949, 1217
HphI GGTGA 5 cut(s) 53, 239, 674, 1271, 1352
Hpy166II GTNNAC 4 cut(s) 206, 275, 1222, 1287
Hpy188I TCNGA 7 cut(s) 174, 349, 547, 709, 1153, 1474, 1486
Hpy188III TCNNGA 8 cut(s) 241, 383, 719, 794, 875, 1070, 1162, 1479
Hpy8I GTNNAC 4 cut(s) 206, 275, 1222, 1287
Hpy99I CGWCG 3 cut(s) 420, 427, 639
HpyAV CCTTC 5 cut(s) 52, 351, 446, 513, 725
HpyCH4III ACNGT 1 cut(s) 1347
HpyCH4IV ACGT 1 cut(s) 634
HpyCH4V TGCA 3 cut(s) 405, 1019, 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 626, 1025, 1189, 1390, 1451
HpySE526I ACGT 1 cut(s) 634
Hsp92II CATG 1 cut(s) 409
HspAI GCGC 3 cut(s) 197, 400, 1183
KflI GGGWCCC 1 cut(s) 640
Kzo9I GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
LmnI GCTCC 8 cut(s) 61, 481, 557, 568, 625, 910, 1381, 1390
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 2 cut(s) 1321, 1423
MaeI CTAG 6 cut(s) 18, 444, 531, 773, 996, 1137
MaeII ACGT 1 cut(s) 634
MalI GATC 7 cut(s) 245, 375, 501, 723, 831, 1074, 1483
MboI GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
MboII GAAGA 8 cut(s) 74, 288, 336, 572, 803, 1058, 1089, 1488
MflI RGATCY 3 cut(s) 499, 1072, 1481
MhlI GDGCHC 4 cut(s) 390, 459, 1278, 1447
MluCI AATT 6 cut(s) 25, 231, 324, 679, 753, 1046
MseI TTAA 3 cut(s) 858, 918, 1522
MspI CCGG 5 cut(s) 14, 311, 566, 949, 1217
MspR9I CCNGG 5 cut(s) 105, 138, 950, 984, 1379
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 4 cut(s) 105, 138, 984, 1379
MwoI GCNNNNNNNGC 5 cut(s) 626, 1025, 1189, 1390, 1451
NciI CCSGG 1 cut(s) 950
NdeII GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 1 cut(s) 409
NspI RCATGY 1 cut(s) 409
PceI AGGCCT 2 cut(s) 430, 1421
PctI GAATGC 1 cut(s) 1030
PfeI GAWTC 4 cut(s) 70, 922, 988, 1463
PflFI GACNNNGTC 1 cut(s) 182
PkrI GCNGC 2 cut(s) 110, 404
PpuMI RGGWCCY 1 cut(s) 640
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 4 cut(s) 103, 136, 982, 1377
PspFI CCCAGC 3 cut(s) 191, 199, 1439
PspGI CCWGG 4 cut(s) 103, 136, 982, 1377
PspOMI GGGCCC 2 cut(s) 455, 1443
PspPPI RGGWCCY 1 cut(s) 640
PstNI CAGNNNCTG 3 cut(s) 188, 383, 1484
PsuI RGATCY 3 cut(s) 499, 1072, 1481
PsyI GACNNNGTC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 858, 918, 1522
SatI GCNGC 2 cut(s) 109, 403
Sau3AI GATC 7 cut(s) 243, 373, 499, 721, 829, 1072, 1481
ScrFI CCNGG 5 cut(s) 105, 138, 950, 984, 1379
SduI GDGCHC 4 cut(s) 390, 459, 1278, 1447
SfaNI GCATC 2 cut(s) 1321, 1423
SfcI CTRYAG 2 cut(s) 603, 1449
SinI GGWCC 5 cut(s) 178, 275, 360, 447, 640
SmlI CTYRAG 1 cut(s) 1280
SmoI CTYRAG 1 cut(s) 1280
Sse9I AATT 6 cut(s) 25, 231, 324, 679, 753, 1046
SseBI AGGCCT 2 cut(s) 430, 1421
SsiI CCGC 1 cut(s) 108
SspMI CTAG 6 cut(s) 18, 444, 531, 773, 996, 1137
StuI AGGCCT 2 cut(s) 430, 1421
StyD4I CCNGG 5 cut(s) 103, 136, 948, 982, 1377
StyI CCWWGG 2 cut(s) 443, 624
TaaI ACNGT 1 cut(s) 1347
TaiI ACGT 1 cut(s) 637
TaqI TCGA 4 cut(s) 5, 418, 425, 595
TaqII GACCGA 2 cut(s) 377, 464
TasI AATT 6 cut(s) 25, 231, 324, 679, 753, 1046
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 4 cut(s) 70, 922, 988, 1463
Tru1I TTAA 3 cut(s) 858, 918, 1522
Tru9I TTAA 3 cut(s) 858, 918, 1522
TscAI CASTG 5 cut(s) 133, 271, 1330, 1335, 1507
TseI GCWGC 1 cut(s) 402
TspDTI ATGAA 6 cut(s) 60, 71, 572, 777, 779, 1039
TspGWI ACGGA 1 cut(s) 1393
TspRI CASTG 5 cut(s) 133, 271, 1330, 1335, 1507
Tth111I GACNNNGTC 1 cut(s) 182
VpaK11BI GGWCC 5 cut(s) 178, 275, 360, 447, 640
XapI RAATTY 3 cut(s) 25, 231, 1046
XceI RCATGY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmaJI CCTAGG 1 cut(s) 443
XspI CTAG 6 cut(s) 18, 444, 531, 773, 996, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.