Rmu_sc0002985.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002985.1
OG0001194
Physical Location & Seq
Reverse (-)
39467 .. 40897
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002985.1_g000015.1.cds

Sequence Viewer

Length: 1431 bp
atggctctaaccaagcatgttgccatcattgatgctgcactatcacgccgccgccatcccgattcctcccagtccactccgccgcagagtcgatcttctcgtctgaaccgcgttggcaaagacaccaggcctcctacctgtgccgccgctactcaacatctcaagactcctctgtcgagtccagttgcctcccccgtgccttccgcttctgccgctgccactccattccgctcggcctctccaccgccttcgtctccatctcttgcagcacctctttctgaggatgactcgtctcgcggtaccaagcgccgccttgcggctccttcctctgtcgggccctctatgtctccccacaactctctcaagtttgtgccccagaaatcggcctcatctcgagtctccaagcagcttgcctccctaacttgtaaggtctcctctcgagcatactttcgtacccctgacgagcagcaactttatgaacaatttacttgtcttcgacccattgtttatgaaaagagattcgatttggcctctctagatcctatgtttggcaatatcgttcgggtttttcagtgggttcccttagtggagtctctctcatgtcctaatttatctgttgtgagggaattttatgctaattgctcacctaacatgccctcgagtgttggattgcttgactctgccactccctgtgactgtatggtcagtgtgcgtggcatgtccattgcttttactcctactgccatccgaactgtcatgaatcttcccaggccttatgaggatactctcatcactgctgctacctttcttgcctctctgactctagaactgttggtggatatcatgtatactaatcctcctgatccgcctcctaaggagctagttggcggtgatttcactgaatggtatcggttgcttggtcatctggttcgcactgtcctatatccatccactcaaacaagcaagattacttgggaagaagctcgcttgatgtatactcttgacagtcagggtgccttaattccggccgagcactatatctaccaagccgtgcaaagggctgctgtggccggtcgttgtcaccagcaggccgtgcttgtgtttccttctattatcactgccttgtgctgtcattctcaggtccgctttgccgacgatgatgagcccggtcctgagctcagtgtgtttaatcgctcttctcttaattggggcaccagtcagtgcaagtctcggggtgtgtttgttgaaaccactacggttcttcggtttctagaggaccttggttctcgtcttgactctcgtatgagtcgtctcgaacaggaagttggttctcttagtgcaaatatgtctaccgtacttctccgttccagtgctcctgcgccttctcctagtactgatcctgctagtgttggtgatcttgatgctgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

476

Amino Acids

51.79

Weight (kDa)

8.26

Isoelectric Point (pI)

66.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 172, 701
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 3 cut(s) 299, 1013, 1211
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 3 cut(s) 848, 995, 1349
AccII CGCG 2 cut(s) 111, 297
AclWI GGATC 3 cut(s) 533, 857, 1391
AcoI YGGCCR 2 cut(s) 1026, 1068
AcsI RAATTY 1 cut(s) 626
AfaI GTAC 4 cut(s) 301, 454, 1356, 1393
AfiI CCNNNNNNNGG 5 cut(s) 316, 333, 382, 548, 1056
AgsI TTSAA 1 cut(s) 1247
AjnI CCWGG 2 cut(s) 125, 767
AjuI GAANNNNNNNTTGG 2 cut(s) 1308, 1340
AluBI AGCT 4 cut(s) 409, 880, 983, 1177
AluI AGCT 4 cut(s) 409, 880, 983, 1177
Alw21I GWGCWC 3 cut(s) 1035, 1179, 1375
Alw26I GTCTC 8 cut(s) 258, 297, 351, 403, 436, 597, 1233, 1316
AlwI GGATC 3 cut(s) 533, 857, 1391
Ama87I CYCGRG 4 cut(s) 393, 438, 658, 1230
AoxI GGCC 9 cut(s) 128, 234, 335, 384, 528, 770, 1026, 1068, 1089
ApaI GGGCCC 1 cut(s) 339
ApeKI GCWGC 7 cut(s) 35, 215, 266, 406, 466, 797, 1061
ApoI RAATTY 1 cut(s) 626
Asp718I GGTACC 1 cut(s) 299
AspLEI GCGC 2 cut(s) 309, 1381
AspS9I GGNCC 5 cut(s) 335, 336, 1141, 1169, 1276
AsuC2I CCSGG 1 cut(s) 1167
AsuHPI GGTGA 4 cut(s) 636, 902, 1073, 1424
AvaI CYCGRG 4 cut(s) 393, 438, 658, 1230
AvaII GGWCC 3 cut(s) 1141, 1169, 1276
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 3 cut(s) 339, 375, 1214
BaeI ACNNNNGTAYC 2 cut(s) 436, 469
BanI GGYRCC 3 cut(s) 299, 1013, 1211
BanII GRGCYC 3 cut(s) 339, 1167, 1179
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 3 cut(s) 1035, 1179, 1375
BbvI GCAGC 7 cut(s) 22, 202, 278, 418, 478, 784, 1048
BccI CCATC 5 cut(s) 32, 63, 265, 752, 955
BceAI ACGGC 2 cut(s) 1034, 1076
BciT130I CCWGG 2 cut(s) 127, 769
BciVI GTATCC 1 cut(s) 775
BcnI CCSGG 1 cut(s) 1167
BcoDI GTCTC 8 cut(s) 258, 297, 351, 403, 436, 597, 1233, 1316
BfaI CTAG 6 cut(s) 536, 824, 881, 1271, 1389, 1404
BfoI RGCGCY 1 cut(s) 310
BfuI GTATCC 1 cut(s) 775
BmcAI AGTACT 1 cut(s) 1393
Bme1390I CCNGG 3 cut(s) 127, 769, 1167
Bme18I GGWCC 3 cut(s) 1141, 1169, 1276
BmeT110I CYCGRG 4 cut(s) 393, 438, 658, 1230
BmgT120I GGNCC 5 cut(s) 335, 336, 1141, 1169, 1276
BmiI GGNNCC 6 cut(s) 301, 321, 337, 579, 1015, 1213
BmrFI CCNGG 3 cut(s) 127, 769, 1167
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 22, 1411
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 485
BplI GAGNNNNNCTC 4 cut(s) 272, 304, 581, 613
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 2 cut(s) 146, 347
BpuMI CCSGG 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 767, 1279
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 841, 871
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 333, 382, 548, 1056
Bse118I RCCGGY 1 cut(s) 1070
Bse1I ACTGG 4 cut(s) 70, 182, 1215, 1368
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 2 cut(s) 127, 769
BseDI CCNNGG 2 cut(s) 767, 1279
BseGI GGATG 4 cut(s) 55, 289, 744, 947
BseLI CCNNNNNNNGG 5 cut(s) 316, 333, 382, 548, 1056
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 4 cut(s) 270, 1151, 1164, 1192
BseNI ACTGG 4 cut(s) 70, 182, 1215, 1368
BseRI GAGGAG 2 cut(s) 159, 424
BseSI GKGCMC 3 cut(s) 339, 375, 1214
BseX3I CGGCCG 1 cut(s) 1026
BseXI GCAGC 7 cut(s) 22, 202, 278, 418, 478, 784, 1048
BsgI GTGCAG 1 cut(s) 21
Bsh1236I CGCG 2 cut(s) 111, 297
Bsh1285I CGRYCG 2 cut(s) 1029, 1075
BshFI GGCC 9 cut(s) 130, 236, 337, 386, 530, 772, 1028, 1070, 1091
BshNI GGYRCC 3 cut(s) 299, 1013, 1211
BsiEI CGRYCG 2 cut(s) 1029, 1075
BsiHKAI GWGCWC 3 cut(s) 1035, 1179, 1375
BsiHKCI CYCGRG 4 cut(s) 393, 438, 658, 1230
BsiSI CCGG 3 cut(s) 1025, 1071, 1167
BslI CCNNNNNNNGG 5 cut(s) 316, 333, 382, 548, 1056
BsmAI GTCTC 8 cut(s) 258, 297, 351, 403, 436, 597, 1233, 1316
BsmBI CGTCTC 3 cut(s) 258, 297, 1316
BsnI GGCC 9 cut(s) 130, 236, 337, 386, 530, 772, 1028, 1070, 1091
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 4 cut(s) 393, 438, 658, 1230
Bsp120I GGGCCC 1 cut(s) 335
Bsp1286I GDGCHC 7 cut(s) 339, 375, 1035, 1167, 1179, 1214, 1375
Bsp143I GATC 5 cut(s) 92, 538, 862, 1396, 1414
BspANI GGCC 9 cut(s) 130, 236, 337, 386, 530, 772, 1028, 1070, 1091
BspCNI CTCAG 4 cut(s) 271, 1150, 1165, 1191
BspFNI CGCG 2 cut(s) 111, 297
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 6 cut(s) 301, 321, 337, 579, 1015, 1213
BspPI GGATC 3 cut(s) 533, 857, 1391
BspQI GCTCTTC 1 cut(s) 1201
BspT107I GGYRCC 3 cut(s) 299, 1013, 1211
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 1070
BsrI ACTGG 4 cut(s) 70, 182, 1215, 1368
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 2 cut(s) 767, 1279
BssMI GATC 5 cut(s) 92, 538, 862, 1396, 1414
BssNAI GTATAC 2 cut(s) 849, 996
BssT1I CCWWGG 1 cut(s) 1279
Bst1107I GTATAC 2 cut(s) 849, 996
Bst2UI CCWGG 2 cut(s) 127, 769
Bst4CI ACNGT 7 cut(s) 698, 754, 831, 937, 1007, 1258, 1354
Bst6I CTCTTC 1 cut(s) 1201
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstC8I GCNNGC 3 cut(s) 411, 985, 1089
BstDEI CTNAG 7 cut(s) 279, 583, 873, 1137, 1173, 1178, 1334
BstF5I GGATG 4 cut(s) 55, 289, 744, 947
BstFNI CGCG 2 cut(s) 111, 297
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 2 cut(s) 309, 1381
BstKTI GATC 5 cut(s) 95, 541, 865, 1399, 1417
BstMAI GTCTC 8 cut(s) 258, 297, 351, 403, 436, 597, 1233, 1316
BstMBI GATC 5 cut(s) 92, 538, 862, 1396, 1414
BstMCI CGRYCG 2 cut(s) 1029, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 212, 1067, 1093
BstNI CCWGG 2 cut(s) 127, 769
BstNSI RCATGY 3 cut(s) 20, 655, 721
BstSCI CCNGG 3 cut(s) 125, 767, 1165
BstSLI GKGCMC 3 cut(s) 339, 375, 1214
BstUI CGCG 2 cut(s) 111, 297
BstV1I GCAGC 7 cut(s) 22, 202, 278, 418, 478, 784, 1048
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BstZ17I GTATAC 2 cut(s) 849, 996
BstZI CGGCCG 1 cut(s) 1026
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 9 cut(s) 130, 236, 337, 386, 530, 772, 1028, 1070, 1091
BtsCI GGATG 4 cut(s) 55, 289, 744, 947
BtsI GCAGTG 2 cut(s) 792, 1116
BtsIMutI CAGTG 9 cut(s) 578, 712, 792, 897, 933, 1116, 1186, 1226, 1375
Cac8I GCNNGC 3 cut(s) 411, 985, 1089
CciI TCATGA 1 cut(s) 756
CfoI GCGC 2 cut(s) 309, 1381
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 5 cut(s) 335, 336, 1141, 1169, 1276
Csp6I GTAC 4 cut(s) 300, 453, 1355, 1392
CviAII CATG 6 cut(s) 17, 600, 652, 718, 757, 844
CviQI GTAC 4 cut(s) 300, 453, 1355, 1392
DdeI CTNAG 7 cut(s) 279, 583, 873, 1137, 1173, 1178, 1334
DpnI GATC 5 cut(s) 94, 540, 864, 1398, 1416
DpnII GATC 5 cut(s) 92, 538, 862, 1396, 1414
DrdI GACNNNNNNGTC 2 cut(s) 172, 701
DseDI GACNNNNNNGTC 2 cut(s) 172, 701
EaeI YGGCCR 2 cut(s) 1026, 1068
EagI CGGCCG 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 1201
EarI CTCTTC 1 cut(s) 1201
EciI GGCGGA 2 cut(s) 69, 855
Ecl136II GAGCTC 1 cut(s) 1177
EclXI CGGCCG 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 1279
Eco147I AGGCCT 2 cut(s) 130, 772
Eco24I GRGCYC 3 cut(s) 339, 1167, 1179
Eco31I GGTCTC 1 cut(s) 436
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 3 cut(s) 1141, 1169, 1276
Eco52I CGGCCG 1 cut(s) 1026
Eco53kI GAGCTC 1 cut(s) 1177
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 4 cut(s) 393, 438, 658, 1230
EcoICRI GAGCTC 1 cut(s) 1177
EcoO109I RGGNCCY 2 cut(s) 336, 1276
EcoRII CCWGG 2 cut(s) 125, 767
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 1279
EcoT38I GRGCYC 3 cut(s) 339, 1167, 1179
ErhI CCWWGG 1 cut(s) 1279
Esp3I CGTCTC 3 cut(s) 258, 297, 1316
FaeI CATG 6 cut(s) 20, 603, 655, 721, 760, 847
FatI CATG 6 cut(s) 16, 599, 651, 717, 756, 843
FblI GTMKAC 3 cut(s) 848, 995, 1349
FokI GGATG 4 cut(s) 42, 296, 731, 934
FriOI GRGCYC 3 cut(s) 339, 1167, 1179
FspBI CTAG 6 cut(s) 536, 824, 881, 1271, 1389, 1404
GlaI GCGC 2 cut(s) 308, 1380
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 9 cut(s) 130, 236, 337, 386, 530, 772, 1028, 1070, 1091
HapII CCGG 3 cut(s) 1025, 1071, 1167
HhaI GCGC 2 cut(s) 309, 1381
Hin1II CATG 6 cut(s) 20, 603, 655, 721, 760, 847
Hin6I GCGC 2 cut(s) 307, 1379
HinP1I GCGC 2 cut(s) 307, 1379
HpaII CCGG 3 cut(s) 1025, 1071, 1167
HphI GGTGA 4 cut(s) 636, 902, 1073, 1424
Hpy166II GTNNAC 4 cut(s) 75, 849, 996, 1350
Hpy188I TCNGA 4 cut(s) 105, 280, 749, 819
Hpy8I GTNNAC 4 cut(s) 75, 849, 996, 1350
Hpy99I CGWCG 1 cut(s) 1157
HpyAV CCTTC 5 cut(s) 210, 258, 333, 1116, 1392
HpyCH4III ACNGT 7 cut(s) 698, 754, 831, 937, 1007, 1258, 1354
HpyCH4V TGCA 5 cut(s) 38, 266, 1054, 1224, 1340
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 1067, 1093
HpyF3I CTNAG 7 cut(s) 279, 583, 873, 1137, 1173, 1178, 1334
Hsp92II CATG 6 cut(s) 20, 603, 655, 721, 760, 847
HspAI GCGC 2 cut(s) 307, 1379
KpnI GGTACC 1 cut(s) 303
Kzo9I GATC 5 cut(s) 92, 538, 862, 1396, 1414
LguI GCTCTTC 1 cut(s) 1201
LmnI GCTCC 3 cut(s) 325, 877, 1378
Lsp1109I GCAGC 7 cut(s) 22, 202, 278, 418, 478, 784, 1048
LweI GCATC 2 cut(s) 22, 1411
MaeI CTAG 6 cut(s) 536, 824, 881, 1271, 1389, 1404
MaeIII GTNAC 2 cut(s) 692, 1079
MalI GATC 5 cut(s) 94, 540, 864, 1398, 1416
MbiI CCGCTC 1 cut(s) 231
MboI GATC 5 cut(s) 92, 538, 862, 1396, 1414
MboII GAAGA 6 cut(s) 87, 485, 755, 989, 1188, 1253
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 7 cut(s) 339, 375, 1035, 1167, 1179, 1214, 1375
MluCI AATT 6 cut(s) 482, 607, 626, 637, 1020, 1204
MmeI TCCRAC 1 cut(s) 646
MseI TTAA 3 cut(s) 1019, 1188, 1203
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 3 cut(s) 1025, 1071, 1167
MspR9I CCNGG 3 cut(s) 127, 769, 1167
MvaI CCWGG 2 cut(s) 127, 769
MvnI CGCG 2 cut(s) 111, 297
MwoI GCNNNNNNNGC 3 cut(s) 212, 1067, 1093
NciI CCSGG 1 cut(s) 1167
NdeII GATC 5 cut(s) 92, 538, 862, 1396, 1414
NlaIII CATG 6 cut(s) 20, 603, 655, 721, 760, 847
NlaIV GGNNCC 6 cut(s) 301, 321, 337, 579, 1015, 1213
NmeAIII GCCGAG 2 cut(s) 212, 1054
NmuCI GTSAC 2 cut(s) 692, 1079
NspI RCATGY 3 cut(s) 20, 655, 721
PaeR7I CTCGAG 3 cut(s) 393, 438, 658
PagI TCATGA 1 cut(s) 756
PceI AGGCCT 2 cut(s) 130, 772
PciSI GCTCTTC 1 cut(s) 1201
PcsI WCGNNNNNNNCGW 2 cut(s) 97, 1306
PfeI GAWTC 3 cut(s) 62, 519, 760
PpuMI RGGWCCY 1 cut(s) 1276
Psp124BI GAGCTC 1 cut(s) 1179
Psp5II RGGWCCY 1 cut(s) 1276
Psp6I CCWGG 2 cut(s) 125, 767
PspGI CCWGG 2 cut(s) 125, 767
PspN4I GGNNCC 6 cut(s) 301, 321, 337, 579, 1015, 1213
PspOMI GGGCCC 1 cut(s) 335
PspPI GGNCC 5 cut(s) 335, 336, 1141, 1169, 1276
PspPPI RGGWCCY 1 cut(s) 1276
PspXI VCTCGAGB 1 cut(s) 658
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 4 cut(s) 301, 454, 1356, 1393
RsaNI GTAC 4 cut(s) 300, 453, 1355, 1392
SacI GAGCTC 1 cut(s) 1179
SapI GCTCTTC 1 cut(s) 1201
SaqAI TTAA 3 cut(s) 1019, 1188, 1203
Sau3AI GATC 5 cut(s) 92, 538, 862, 1396, 1414
Sau96I GGNCC 5 cut(s) 335, 336, 1141, 1169, 1276
ScaI AGTACT 1 cut(s) 1393
ScrFI CCNGG 3 cut(s) 127, 769, 1167
SduI GDGCHC 7 cut(s) 339, 375, 1035, 1167, 1179, 1214, 1375
SfaNI GCATC 2 cut(s) 22, 1411
Sfr274I CTCGAG 3 cut(s) 393, 438, 658
SinI GGWCC 3 cut(s) 1141, 1169, 1276
SlaI CTCGAG 3 cut(s) 393, 438, 658
SmlI CTYRAG 5 cut(s) 161, 362, 393, 438, 658
SmoI CTYRAG 5 cut(s) 161, 362, 393, 438, 658
Sse9I AATT 6 cut(s) 482, 607, 626, 637, 1020, 1204
SseBI AGGCCT 2 cut(s) 130, 772
SspMI CTAG 6 cut(s) 536, 824, 881, 1271, 1389, 1404
SstI GAGCTC 1 cut(s) 1179
StuI AGGCCT 2 cut(s) 130, 772
StyD4I CCNGG 3 cut(s) 125, 767, 1165
StyI CCWWGG 1 cut(s) 1279
TaaI ACNGT 7 cut(s) 698, 754, 831, 937, 1007, 1258, 1354
TaqI TCGA 8 cut(s) 91, 176, 394, 439, 496, 522, 659, 1314
TasI AATT 6 cut(s) 482, 607, 626, 637, 1020, 1204
TatI WGTACW 1 cut(s) 1391
TauI GCSGC 8 cut(s) 51, 54, 85, 146, 149, 215, 312, 320
TfiI GAWTC 3 cut(s) 62, 519, 760
Tru1I TTAA 3 cut(s) 1019, 1188, 1203
Tru9I TTAA 3 cut(s) 1019, 1188, 1203
TscAI CASTG 9 cut(s) 578, 712, 799, 904, 940, 1123, 1186, 1226, 1375
TseFI GTSAC 2 cut(s) 692, 1079
TseI GCWGC 7 cut(s) 35, 215, 266, 406, 466, 797, 1061
Tsp45I GTSAC 2 cut(s) 692, 1079
TspDTI ATGAA 3 cut(s) 492, 525, 773
TspGWI ACGGA 1 cut(s) 1352
TspRI CASTG 9 cut(s) 578, 712, 799, 904, 940, 1123, 1186, 1226, 1375
VpaK11BI GGWCC 3 cut(s) 1141, 1169, 1276
XapI RAATTY 1 cut(s) 626
XbaI TCTAGA 3 cut(s) 535, 823, 1270
XceI RCATGY 3 cut(s) 20, 655, 721
XhoI CTCGAG 3 cut(s) 393, 438, 658
XmiI GTMKAC 3 cut(s) 848, 995, 1349
XspI CTAG 6 cut(s) 536, 824, 881, 1271, 1389, 1404
ZrmI AGTACT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.