Rroxscaffold_1G00020530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001194
Physical Location & Seq
Reverse (-)
24883444 .. 24883906
463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020530.1

Sequence Viewer

Length: 357 bp
ATGTCTCGTTCCTTTTACTCCTATCTCCATTCGAGTGCCCTGGGTTTGGTTGCTCCTATAGATGAGATTTTTGAGGTAGATGCTACTCTTGAAGTGTATGAGGAACATTCGGTTGTGACACCCGAAGAATTGTCCGCTAGTGCTCTCACCCGCAGTTCCGCTGCCGGGTCACTTGGTGCGCACTCGTTCCATCCGAAGGCGCTATTGAGGGTCGTCGTCACTGAAGGTCGCCTTGGTTTTCCTTCGCCTATCACCGCTGTTTGCAAGGCCAAGGGTGTTAATTTTGCTGATGATGATGAATATGGTCCAACCTTGAAGATCTTCTCTTGCCGTTCCCTCCACCGTAGTACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

12.7

Weight (kDa)

5.66

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 180
AciI CCGC 4 cut(s) 135, 151, 159, 255
AcuI CTGAAG 1 cut(s) 243
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 1 cut(s) 349
AfiI CCNNNNNNNGG 3 cut(s) 46, 165, 196
AgsI TTSAA 2 cut(s) 92, 316
AhlI ACTAGT 1 cut(s) 350
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 9
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 181, 202
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 139, 244
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 145
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 198
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 1 cut(s) 41
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 9
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 138, 351
BfmI CTRYAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 203
BglII AGATCT 1 cut(s) 318
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 2 cut(s) 41, 166
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmrFI CCNGG 2 cut(s) 41, 166
BmsI GCATC 1 cut(s) 70
BpuMI CCSGG 1 cut(s) 166
BsaJI CCNNGG 4 cut(s) 39, 40, 232, 270
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 165, 196
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 4 cut(s) 39, 40, 232, 270
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 3 cut(s) 46, 165, 196
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 1 cut(s) 148
BshFI GGCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 3 cut(s) 46, 165, 196
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 40, 145
Bsp143I GATC 1 cut(s) 318
BspACI CCGC 4 cut(s) 135, 151, 159, 255
BspANI GGCC 1 cut(s) 269
BssECI CCNNGG 4 cut(s) 39, 40, 232, 270
BssMI GATC 1 cut(s) 318
BssT1I CCWWGG 2 cut(s) 232, 270
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 344
BstF5I GGATG 1 cut(s) 190
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 181, 202
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 318
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 39, 164
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 219
CfoI GCGC 2 cut(s) 181, 202
Cfr13I GGNCC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 348
CviJI RGCY 1 cut(s) 269
CviKI_1 RGCY 1 cut(s) 269
CviQI GTAC 1 cut(s) 348
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
DraIII CACNNNGTG 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 232, 270
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 2 cut(s) 232, 270
ErhI CCWWGG 2 cut(s) 232, 270
FaiI YATR 3 cut(s) 59, 99, 303
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FauI CCCGC 1 cut(s) 158
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 162
FspAI RTGCGCAY 1 cut(s) 180
FspBI CTAG 2 cut(s) 138, 351
FspI TGCGCA 1 cut(s) 180
GlaI GCGC 2 cut(s) 180, 201
GluI GCNGC 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 1 cut(s) 269
HapII CCGG 1 cut(s) 165
HhaI GCGC 2 cut(s) 181, 202
Hin6I GCGC 2 cut(s) 179, 200
HinP1I GCGC 2 cut(s) 179, 200
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 139, 244
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 1 cut(s) 89
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 190, 218, 252
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 1 cut(s) 264
HspAI GCGC 2 cut(s) 179, 200
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 58
LpnPI CCDG 3 cut(s) 26, 53, 178
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 70
MaeI CTAG 2 cut(s) 138, 351
MaeIII GTNAC 3 cut(s) 115, 168, 217
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 3 cut(s) 137, 313, 328
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 40, 145
MluCI AATT 2 cut(s) 128, 280
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 4 cut(s) 67, 94, 201, 347
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 2 cut(s) 279, 355
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 2 cut(s) 161, 257
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 41, 166
MvaI CCWGG 1 cut(s) 41
NciI CCSGG 1 cut(s) 166
NdeII GATC 1 cut(s) 318
NmuCI GTSAC 3 cut(s) 115, 168, 217
NsbI TGCGCA 1 cut(s) 180
PasI CCCWGGG 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 320
PkrI GCNGC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 1 cut(s) 349
RsaNI GTAC 1 cut(s) 348
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 2 cut(s) 279, 355
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 1 cut(s) 305
ScaI AGTACT 1 cut(s) 349
ScrFI CCNGG 2 cut(s) 41, 166
SduI GDGCHC 2 cut(s) 40, 145
SetI ASST 3 cut(s) 78, 229, 314
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 33
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 2 cut(s) 128, 280
SsiI CCGC 4 cut(s) 135, 151, 159, 255
SspMI CTAG 2 cut(s) 138, 351
StyD4I CCNGG 2 cut(s) 39, 164
StyI CCWWGG 2 cut(s) 232, 270
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 128, 280
TatI WGTACW 1 cut(s) 347
Tru1I TTAA 2 cut(s) 279, 355
Tru9I TTAA 2 cut(s) 279, 355
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 3 cut(s) 115, 168, 217
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 3 cut(s) 115, 168, 217
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 138, 351
ZrmI AGTACT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.