Rmu_sc0002012.1_g000020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002012.1
OG0001194
Physical Location & Seq
Reverse (-)
119416 .. 120789
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002012.1_g000020.1.cds

Sequence Viewer

Length: 1374 bp
atggctcgcatcaagcacaccgttccgcgacccaaagcttcgaccattgcctcgcctccggctccgcctccagccccccgtcctactcggcgttccaatcgccttcacctgcctcgagacgctggccctcctacctccgctgcgaatcctcaggccttgccggactcgccatctcagtctccggcccgtgctccatcatcgacttctgggtccgagcattcctccaccgccgctgctctttccgattccgctgcttctgagcctgctcctgcttctccctcaccgactcccacaccgccgcgccgctcgcctagtccgtccataactgctccgccatccaagcgacctcggacctccggccgctctgctccttccaccttcaggtcgcccaagtctcctacaccccgtaaggattctcgctttggcactcgtactcaaagacttgattatgaaaattttacatgtttccgacccttggtctatgaaaagcgtttcaatatcgctgagttgggtactttgtttgccgaaactgtgagactttttcgttgggaatctgtattggatatgctaccaactcctaaccttactgtggtcagagagttctatgcttgttgttctccaaatatgccaatgcaggttgatttgcttaatgcggctactctgattggctgcaatgtggatattcgcggtgtctccgtccctttcacccccaccaccatccgcgacactttgggtctccttgcccctgttaccgagatgcctaagattgatcaagaacttgctgctttgtccactgatactgttgtggctgccctatatgaggatcgtccggatgccacacccatggagctttccgccggtgctctcagtgagttttgccgcttgttgggacatttggttcgtactctgctttatccttctactcagagcagcaagattaccttggaggaagctcgactcatctatgtcctcaatgccaaaggtccctaccttcctcctgagcacttcatgtatactgctatccggaaagctgctgttgagggccgtcgacacaagaaggctgctttagtttttccctctgtgatcactgctgtctgcaaagccaagggggttgtctttaaggactctgatgacatggcccctcccctgaaagttttctcccggagttctctcctccgcagctccagtcaaagcaagaccaagggtatctctgttgacatcacttctatctctcggaccttgcttgaccatttcacggtggtgaatcgcaagttggatcagcttcagactgatgtgggtgctcttcttcagcgtgtggttcatcttgagggggagtttcactctcttcgtgacgatcttgacgcttctccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

457

Amino Acids

49.67

Weight (kDa)

9.69

Isoelectric Point (pI)

65.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 117
AasI GACNNNNNNGTC 1 cut(s) 208
Acc36I ACCTGC 2 cut(s) 117, 625
AccBSI CCGCTC 2 cut(s) 306, 363
AccI GTMKAC 2 cut(s) 1013, 1048
AccII CGCG 4 cut(s) 28, 301, 687, 723
AccIII TCCGGA 2 cut(s) 829, 1023
AclWI GGATC 2 cut(s) 831, 1284
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 1 cut(s) 454
AcuI CTGAAG 3 cut(s) 364, 1268, 1292
AfaI GTAC 3 cut(s) 433, 514, 904
AfiI CCNNNNNNNGG 6 cut(s) 381, 475, 589, 820, 886, 1255
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 1 cut(s) 496
AhdI GACNNNNNGTC 1 cut(s) 476
AjuI GAANNNNNNNTTGG 2 cut(s) 1256, 1288
AleI CACNNNNGTG 1 cut(s) 1259
AloI GAACNNNNNNTCC 2 cut(s) 882, 914
AluBI AGCT 6 cut(s) 38, 850, 953, 1031, 1182, 1282
AluI AGCT 6 cut(s) 38, 850, 953, 1031, 1182, 1282
Alw21I GWGCWC 4 cut(s) 193, 865, 1005, 1303
Alw26I GTCTC 6 cut(s) 111, 183, 399, 529, 697, 740
AlwI GGATC 2 cut(s) 831, 1284
Ama87I CYCGRG 1 cut(s) 114
Aor13HI TCCGGA 2 cut(s) 829, 1023
AoxI GGCC 6 cut(s) 124, 153, 183, 358, 1042, 1137
ApoI RAATTY 1 cut(s) 454
ArsI GACNNNNNNTTYG 2 cut(s) 882, 914
Asp700I GAANNNNTTC 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 8 cut(s) 125, 184, 210, 351, 983, 1042, 1138, 1236
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 4 cut(s) 98, 273, 697, 1273
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 4 cut(s) 210, 351, 983, 1236
AxyI CCTNAGG 1 cut(s) 150
BaeI ACNNNNGTAYC 2 cut(s) 789, 822
Bbv12I GWGCWC 4 cut(s) 193, 865, 1005, 1303
BccI CCATC 4 cut(s) 178, 202, 343, 725
BceAI ACGGC 1 cut(s) 1029
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BclI TGATCA 2 cut(s) 769, 1083
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 6 cut(s) 111, 183, 399, 529, 697, 740
BfaI CTAG 1 cut(s) 312
BfuAI ACCTGC 2 cut(s) 117, 625
Bme1390I CCNGG 1 cut(s) 1162
Bme18I GGWCC 4 cut(s) 210, 351, 983, 1236
BmeRI GACNNNNNGTC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 8 cut(s) 125, 184, 210, 351, 983, 1042, 1138, 1236
BmiI GGNNCC 4 cut(s) 63, 211, 985, 1140
BmrFI CCNGG 1 cut(s) 1162
BmsI GCATC 3 cut(s) 18, 747, 823
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 1325, 1357
BpmI CTGGAG 2 cut(s) 54, 1168
Bpu10I CCTNAGC 1 cut(s) 999
BpuEI CTTGAG 1 cut(s) 1346
BpuMI CCSGG 1 cut(s) 1162
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 6 cut(s) 347, 474, 843, 942, 1104, 1200
BsaWI WCCGGW 2 cut(s) 829, 1023
Bsc4I CCNNNNNNNGG 6 cut(s) 381, 475, 589, 820, 886, 1255
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 1185
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 2 cut(s) 45, 679
BseAI TCCGGA 2 cut(s) 829, 1023
BseDI CCNNGG 6 cut(s) 347, 474, 843, 942, 1104, 1200
BseGI GGATG 3 cut(s) 335, 717, 838
BseLI CCNNNNNNNGG 6 cut(s) 381, 475, 589, 820, 886, 1255
BseMI GCAATG 2 cut(s) 45, 679
BseMII CTCAG 7 cut(s) 164, 188, 249, 495, 880, 938, 990
BseNI ACTGG 1 cut(s) 1185
BseRI GAGGAG 1 cut(s) 1163
BseX3I CGGCCG 1 cut(s) 358
Bsh1236I CGCG 4 cut(s) 28, 301, 687, 723
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 6 cut(s) 126, 155, 185, 360, 1044, 1139
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 4 cut(s) 193, 865, 1005, 1303
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 8 cut(s) 59, 161, 182, 357, 830, 858, 1024, 1162
BslFI GGGAC 3 cut(s) 683, 903, 969
BslI CCNNNNNNNGG 6 cut(s) 381, 475, 589, 820, 886, 1255
BsmAI GTCTC 6 cut(s) 111, 183, 399, 529, 697, 740
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 3 cut(s) 683, 903, 969
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 6 cut(s) 126, 155, 185, 360, 1044, 1139
Bso31I GGTCTC 1 cut(s) 740
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 4 cut(s) 193, 865, 1005, 1303
Bsp13I TCCGGA 2 cut(s) 829, 1023
Bsp143I GATC 5 cut(s) 769, 823, 1083, 1276, 1354
Bsp19I CCATGG 1 cut(s) 843
BspANI GGCC 6 cut(s) 126, 155, 185, 360, 1044, 1139
BspCNI CTCAG 7 cut(s) 163, 187, 250, 496, 879, 937, 991
BspEI TCCGGA 2 cut(s) 829, 1023
BspFNI CGCG 4 cut(s) 28, 301, 687, 723
BspLI GGNNCC 4 cut(s) 63, 211, 985, 1140
BspMI ACCTGC 2 cut(s) 117, 625
BspPI GGATC 2 cut(s) 831, 1284
BspQI GCTCTTC 1 cut(s) 1308
BspTNI GGTCTC 1 cut(s) 740
BsrBI CCGCTC 2 cut(s) 306, 363
BsrDI GCAATG 2 cut(s) 45, 679
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 1 cut(s) 1185
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 6 cut(s) 347, 474, 843, 942, 1104, 1200
BssMI GATC 5 cut(s) 769, 823, 1083, 1276, 1354
BssNAI GTATAC 1 cut(s) 1014
BssT1I CCWWGG 5 cut(s) 474, 843, 942, 1104, 1200
Bst1107I GTATAC 1 cut(s) 1014
Bst4CI ACNGT 5 cut(s) 22, 532, 589, 802, 1258
Bst6I CTCTTC 2 cut(s) 1308, 1350
BstC8I GCNNGC 4 cut(s) 7, 124, 264, 308
BstDEI CTNAG 8 cut(s) 150, 174, 258, 504, 762, 866, 924, 999
BstDSI CCRYGG 1 cut(s) 843
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 3 cut(s) 335, 717, 838
BstFNI CGCG 4 cut(s) 28, 301, 687, 723
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 5 cut(s) 772, 826, 1086, 1279, 1357
BstMAI GTCTC 6 cut(s) 111, 183, 399, 529, 697, 740
BstMBI GATC 5 cut(s) 769, 823, 1083, 1276, 1354
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 3 cut(s) 166, 307, 340
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 1160
BstUI CGCG 4 cut(s) 28, 301, 687, 723
BstXI CCANNNNNNTGG 1 cut(s) 844
BstZ17I GTATAC 1 cut(s) 1014
BstZI CGGCCG 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 6 cut(s) 126, 155, 185, 360, 1044, 1139
BtgI CCRYGG 1 cut(s) 843
BtsCI GGATG 3 cut(s) 335, 717, 838
BtsI GCAGTG 1 cut(s) 1086
BtsIMutI CAGTG 3 cut(s) 792, 874, 1086
BveI ACCTGC 2 cut(s) 117, 625
Cac8I GCNNGC 4 cut(s) 7, 124, 264, 308
CfoI GCGC 1 cut(s) 303
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 8 cut(s) 125, 184, 210, 351, 983, 1042, 1138, 1236
CseI GACGC 1 cut(s) 128
Csp6I GTAC 3 cut(s) 432, 513, 903
CviAII CATG 4 cut(s) 462, 844, 1009, 1135
CviQI GTAC 3 cut(s) 432, 513, 903
DdeI CTNAG 8 cut(s) 150, 174, 258, 504, 762, 866, 924, 999
DpnI GATC 5 cut(s) 771, 825, 1085, 1278, 1356
DpnII GATC 5 cut(s) 769, 823, 1083, 1276, 1354
DrdI GACNNNNNNGTC 1 cut(s) 208
DriI GACNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 358
EagI CGGCCG 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 1308, 1350
Eam1105I GACNNNNNGTC 1 cut(s) 476
EarI CTCTTC 2 cut(s) 1308, 1350
EciI GGCGGA 3 cut(s) 54, 321, 844
EclXI CGGCCG 1 cut(s) 358
Eco130I CCWWGG 5 cut(s) 474, 843, 942, 1104, 1200
Eco147I AGGCCT 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 4 cut(s) 210, 351, 983, 1236
Eco52I CGGCCG 1 cut(s) 358
Eco57I CTGAAG 3 cut(s) 364, 1268, 1292
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 114
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoO109I RGGNCCY 1 cut(s) 983
EcoT14I CCWWGG 5 cut(s) 474, 843, 942, 1104, 1200
ErhI CCWWGG 5 cut(s) 474, 843, 942, 1104, 1200
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 4 cut(s) 465, 847, 1012, 1138
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 3 cut(s) 683, 903, 969
FatI CATG 4 cut(s) 461, 843, 1008, 1134
FbaI TGATCA 2 cut(s) 769, 1083
FblI GTMKAC 2 cut(s) 1013, 1048
FokI GGATG 3 cut(s) 322, 704, 845
FspBI CTAG 1 cut(s) 312
GlaI GCGC 1 cut(s) 302
GsuI CTGGAG 2 cut(s) 54, 1168
HaeIII GGCC 6 cut(s) 126, 155, 185, 360, 1044, 1139
HapII CCGG 8 cut(s) 59, 161, 182, 357, 830, 858, 1024, 1162
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 303
Hin1II CATG 4 cut(s) 465, 847, 1012, 1138
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 2 cut(s) 1049, 1216
HindII GTYRAC 2 cut(s) 1049, 1216
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 9 cut(s) 145, 164, 245, 286, 413, 551, 957, 1124, 1264
HpaII CCGG 8 cut(s) 59, 161, 182, 357, 830, 858, 1024, 1162
HphI GGTGA 4 cut(s) 98, 273, 697, 1273
Hpy166II GTNNAC 4 cut(s) 792, 1014, 1049, 1216
Hpy188III TCNNGA 8 cut(s) 116, 773, 830, 998, 1024, 1325, 1349, 1358
Hpy8I GTNNAC 4 cut(s) 792, 1014, 1049, 1216
Hpy99I CGWCG 1 cut(s) 1050
HpyAV CCTTC 6 cut(s) 113, 381, 388, 927, 1001, 1051
HpyCH4III ACNGT 5 cut(s) 22, 532, 589, 802, 1258
HpyCH4V TGCA 3 cut(s) 634, 672, 1098
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 307, 340
HpyF3I CTNAG 8 cut(s) 150, 174, 258, 504, 762, 866, 924, 999
Hsp92II CATG 4 cut(s) 465, 847, 1012, 1138
HspAI GCGC 1 cut(s) 301
Kpn2I TCCGGA 2 cut(s) 829, 1023
Ksp22I TGATCA 2 cut(s) 769, 1083
Kzo9I GATC 5 cut(s) 769, 823, 1083, 1276, 1354
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 7 cut(s) 67, 196, 271, 334, 373, 847, 1187
LweI GCATC 3 cut(s) 18, 747, 823
MaeI CTAG 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 748, 1349
MalI GATC 5 cut(s) 771, 825, 1085, 1278, 1356
MbiI CCGCTC 2 cut(s) 306, 363
MboI GATC 5 cut(s) 769, 823, 1083, 1276, 1354
MboII GAAGA 3 cut(s) 1295, 1298, 1337
MhlI GDGCHC 4 cut(s) 193, 865, 1005, 1303
MluCI AATT 1 cut(s) 454
MlyI GAGTC 4 cut(s) 158, 280, 951, 1118
MmeI TCCRAC 2 cut(s) 493, 1254
MroI TCCGGA 2 cut(s) 829, 1023
MroXI GAANNNNTTC 1 cut(s) 1154
MseI TTAA 2 cut(s) 648, 1119
MslI CAYNNNNRTG 2 cut(s) 842, 1259
MspA1I CMGCKG 3 cut(s) 140, 233, 251
MspI CCGG 8 cut(s) 59, 161, 182, 357, 830, 858, 1024, 1162
MspR9I CCNGG 1 cut(s) 1162
Mva1269I GAATGC 1 cut(s) 217
MvnI CGCG 4 cut(s) 28, 301, 687, 723
MwoI GCNNNNNNNGC 3 cut(s) 166, 307, 340
NciI CCSGG 1 cut(s) 1162
NcoI CCATGG 1 cut(s) 843
NdeII GATC 5 cut(s) 769, 823, 1083, 1276, 1354
NlaIII CATG 4 cut(s) 465, 847, 1012, 1138
NlaIV GGNNCC 4 cut(s) 63, 211, 985, 1140
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 1349
NspI RCATGY 1 cut(s) 465
OliI CACNNNNGTG 1 cut(s) 1259
PaeR7I CTCGAG 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 117
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 1 cut(s) 461
PciSI GCTCTTC 1 cut(s) 1308
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PctI GAATGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 1154
PfeI GAWTC 5 cut(s) 145, 245, 413, 551, 1264
PfoI TCCNGGA 1 cut(s) 1160
PleI GAGTC 4 cut(s) 158, 280, 951, 1118
PpsI GAGTC 4 cut(s) 158, 280, 951, 1118
PpuMI RGGWCCY 1 cut(s) 983
PscI ACATGT 1 cut(s) 461
Psp5II RGGWCCY 1 cut(s) 983
PspN4I GGNNCC 4 cut(s) 63, 211, 985, 1140
PspPI GGNCC 8 cut(s) 125, 184, 210, 351, 983, 1042, 1138, 1236
PspPPI RGGWCCY 1 cut(s) 983
RsaI GTAC 3 cut(s) 433, 514, 904
RsaNI GTAC 3 cut(s) 432, 513, 903
RseI CAYNNNNRTG 2 cut(s) 842, 1259
SalI GTCGAC 1 cut(s) 1047
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 2 cut(s) 648, 1119
Sau3AI GATC 5 cut(s) 769, 823, 1083, 1276, 1354
Sau96I GGNCC 8 cut(s) 125, 184, 210, 351, 983, 1042, 1138, 1236
SchI GAGTC 4 cut(s) 158, 280, 951, 1118
ScrFI CCNGG 1 cut(s) 1162
SduI GDGCHC 4 cut(s) 193, 865, 1005, 1303
SfaNI GCATC 3 cut(s) 18, 747, 823
Sfr274I CTCGAG 1 cut(s) 114
SgrAI CRCCGGYG 1 cut(s) 857
SinI GGWCC 4 cut(s) 210, 351, 983, 1236
SlaI CTCGAG 1 cut(s) 114
SmiMI CAYNNNNRTG 2 cut(s) 842, 1259
SmlI CTYRAG 2 cut(s) 114, 1325
SmoI CTYRAG 2 cut(s) 114, 1325
Sse9I AATT 1 cut(s) 454
SseBI AGGCCT 1 cut(s) 155
SspMI CTAG 1 cut(s) 312
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 1160
StyI CCWWGG 5 cut(s) 474, 843, 942, 1104, 1200
TaaI ACNGT 5 cut(s) 22, 532, 589, 802, 1258
TaqI TCGA 5 cut(s) 41, 115, 200, 955, 1048
TasI AATT 1 cut(s) 454
TauI GCSGC 6 cut(s) 233, 301, 306, 363, 656, 882
TfiI GAWTC 5 cut(s) 145, 245, 413, 551, 1264
Tru1I TTAA 2 cut(s) 648, 1119
Tru9I TTAA 2 cut(s) 648, 1119
TscAI CASTG 3 cut(s) 799, 874, 1093
TseFI GTSAC 1 cut(s) 1349
Tsp45I GTSAC 1 cut(s) 1349
TspDTI ATGAA 4 cut(s) 465, 498, 997, 1310
TspGWI ACGGA 3 cut(s) 306, 685, 1359
TspRI CASTG 3 cut(s) 799, 874, 1093
VpaK11BI GGWCC 4 cut(s) 210, 351, 983, 1236
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 1 cut(s) 454
XceI RCATGY 1 cut(s) 465
XhoI CTCGAG 1 cut(s) 114
XmiI GTMKAC 2 cut(s) 1013, 1048
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.