FvH4_5g32131

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001194
Physical Location & Seq
Reverse (-)
23209656 .. 23211352
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32131.t1

Sequence Viewer

Length: 1296 bp
ATGGGTGGTTTTGATTCTGGTGGTTCTCAGCCCCAAGCGCCTCCGATGGCTACCCCCTCAAGGGGAGGCTCTTCCTCTGCTGCTGCCTCTCCGGCAGAATCCACCCTTGCCGATGCCCTCTCGGCCATCCGCAAACGCGCCCTCCCTGAGAAGACAAATCCTTCGGCTAAAATGGCCAAAGGCACCGCCTCCTCTGGTGGTGGTCCCTCCTCTGTGGCTTCTGCTGCTCAAGCCTCCCCTTGCTCCCCTGCTCCCCAATCCGACGGGGAATTGCAGCGTTCGGCTCCGGTCTCGGCGTCGGCAGGATCGAAGCAGGCTCGAGTTAAGCGCCCTGCTTCCTGGATTTCTAAGCCTTCCAAGTCTTCTGGGCAGCCCTCTTCTCCTACTGTGAAACTCCCGGCACGGTCCAATGCCCCATCCCAGAAGCCTCCAACGTCCTCCAAATCAGCCAAGGTTTCATCCAAAAACCCTGGTTCTGCTTCTGGTAGTGCCTCTGCTAAGGGTAAGGGTAAAGCCTCCATGTCTGGGAGTGAGGAGATGTATGCTACCATCCCTCCCAAGAAGCGCTCTCATGAGGTATTCTACTCTAAGATTCCCCCTCTTAGTCATTTTGTTGATGAAGATGCTCTTACTTTATTCTGCACTGTGCTTGTGGAAAAGCGAATTGTCTTTGAAAGGTCTTTTGCATTTGATACTTTGCATCCTAAAGTGAAAGAGTTTGTTGAGGATTTGCAGTGGGACCATGCCTTGGCCTCTTTCCCTAAGGCCAAGCCCCTTCGACCTCGTATTCCGGCCGAATATTTTATGTATCAAGCCATGAGAACTGCTGGGATCACCAATCGTTCTTCCAAGAGTTTCTCTCTTGTCTTTCCCGGGCTGATCACCAAGTTATGCTTAGAAGCTGGTGTTACGGTGTGTCCTTTTGATGTCATGGCAAATGATTGTGAGGCTGCCCCTGTTGACAAGCTCACAATTGCTAAGTCGGCTAGTCAATCCAATGTGTATGTTGAACCCATGGAGCAGACTTTTCTAAAGCAAGATATGTCCCTGATGATTGATGAAGCTGTTTCCTCTCTCAAAGCTGCTTTTGCTGAGCAGATTGCCAGTCTCAAGGCTGAATTGTTGGAGTCAGTGGGGAACCGTTCCAATTCTGCCCGGGATGGCACTGATCCCACCCCTCTTGACTTGGGATCTTCCTCTCAGTCTACTGCTAGGGAGTCCTCTGGTTTTGGCTCTGCGAGCTCTTCCTCTGAGCAGTCTTCTGGTGACGCTCTGGATGTCATGGATGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

44.95

Weight (kDa)

8.79

Isoelectric Point (pI)

63.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccB7I CCANNNNNTGG 1 cut(s) 748
AccI GTMKAC 1 cut(s) 1205
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 130, 186
AclWI GGATC 4 cut(s) 313, 839, 1163, 1198
AcoI YGGCCR 3 cut(s) 123, 174, 792
AcyI GRCGYC 1 cut(s) 296
AfeI AGCGCT 1 cut(s) 566
AfiI CCNNNNNNNGG 5 cut(s) 60, 61, 62, 525, 748
AgsI TTSAA 2 cut(s) 674, 1010
AjnI CCWGG 2 cut(s) 338, 469
AluBI AGCT 5 cut(s) 902, 967, 1064, 1082, 1242
AluI AGCT 5 cut(s) 902, 967, 1064, 1082, 1242
Alw21I GWGCWC 1 cut(s) 1244
Alw26I GTCTC 2 cut(s) 295, 1112
AlwI GGATC 4 cut(s) 313, 839, 1163, 1198
Ama87I CYCGRG 3 cut(s) 318, 872, 1155
Aor51HI AGCGCT 1 cut(s) 566
AoxI GGCC 5 cut(s) 123, 174, 750, 765, 792
ApeKI GCWGC 7 cut(s) 80, 83, 224, 274, 370, 950, 1082
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspLEI GCGC 4 cut(s) 40, 140, 330, 567
AspS9I GGNCC 3 cut(s) 203, 405, 739
AsuC2I CCSGG 5 cut(s) 398, 873, 874, 1156, 1157
AsuHPI GGTGA 3 cut(s) 826, 874, 1277
AvaI CYCGRG 3 cut(s) 318, 872, 1155
AvaII GGWCC 3 cut(s) 203, 405, 739
AxyI CCTNAGG 1 cut(s) 762
BalI TGGCCA 1 cut(s) 176
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 1244
BbsI GAAGAC 3 cut(s) 158, 354, 1251
Bbv12I GWGCWC 1 cut(s) 1244
BbvI GCAGC 7 cut(s) 67, 70, 211, 286, 382, 937, 1069
BccI CCATC 5 cut(s) 40, 134, 424, 557, 1154
BciT130I CCWGG 2 cut(s) 340, 471
BclI TGATCA 1 cut(s) 879
BcnI CCSGG 5 cut(s) 398, 873, 874, 1156, 1157
BcoDI GTCTC 2 cut(s) 295, 1112
BfaI CTAG 2 cut(s) 987, 1212
BfoI RGCGCY 3 cut(s) 41, 331, 568
BglI GCCNNNNNGGC 2 cut(s) 92, 122
BisI GCNGC 7 cut(s) 81, 84, 225, 275, 371, 951, 1083
BlpI GCTNAGC 1 cut(s) 1092
BlsI GCNGC 7 cut(s) 82, 85, 226, 276, 372, 952, 1084
Bme1390I CCNGG 7 cut(s) 340, 398, 471, 873, 874, 1156, 1157
Bme18I GGWCC 3 cut(s) 203, 405, 739
BmeT110I CYCGRG 3 cut(s) 318, 872, 1155
BmgT120I GGNCC 3 cut(s) 203, 405, 739
BmiI GGNNCC 5 cut(s) 184, 205, 285, 740, 1139
BmrFI CCNGG 7 cut(s) 340, 398, 471, 873, 874, 1156, 1157
BmsI GCATC 3 cut(s) 103, 613, 709
BpiI GAAGAC 3 cut(s) 158, 354, 1251
BplI GAGNNNNNCTC 2 cut(s) 844, 876
Bpu10I CCTNAGC 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 1092
BpuEI CTTGAG 3 cut(s) 43, 213, 1094
BpuMI CCSGG 5 cut(s) 398, 873, 874, 1156, 1157
BsaHI GRCGYC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 6 cut(s) 450, 469, 747, 872, 1014, 1155
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 538, 568
Bsc4I CCNNNNNNNGG 5 cut(s) 60, 61, 62, 525, 748
Bse1I ACTGG 1 cut(s) 1104
Bse21I CCTNAGG 1 cut(s) 762
BseBI CCWGG 2 cut(s) 340, 471
BseDI CCNNGG 6 cut(s) 450, 469, 747, 872, 1014, 1155
BseGI GGATG 8 cut(s) 126, 416, 458, 549, 700, 1165, 1282, 1291
BseLI CCNNNNNNNGG 5 cut(s) 60, 61, 62, 525, 748
BseMII CTCAG 5 cut(s) 41, 138, 1083, 1214, 1242
BseNI ACTGG 1 cut(s) 1104
BseRI GAGGAG 3 cut(s) 181, 199, 548
BseX3I CGGCCG 1 cut(s) 792
BseXI GCAGC 7 cut(s) 67, 70, 211, 286, 382, 937, 1069
BseYI CCCAGC 1 cut(s) 827
BsgI GTGCAG 1 cut(s) 625
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 5 cut(s) 125, 176, 752, 767, 794
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 1244
BsiHKCI CYCGRG 3 cut(s) 318, 872, 1155
BsiSI CCGG 6 cut(s) 92, 287, 398, 791, 873, 1156
BslFI GGGAC 3 cut(s) 189, 752, 1030
BslI CCNNNNNNNGG 5 cut(s) 60, 61, 62, 525, 748
BsmAI GTCTC 2 cut(s) 295, 1112
BsmFI GGGAC 3 cut(s) 189, 752, 1030
BsnI GGCC 5 cut(s) 125, 176, 752, 767, 794
Bso31I GGTCTC 1 cut(s) 295
BsoBI CYCGRG 3 cut(s) 318, 872, 1155
Bsp1286I GDGCHC 1 cut(s) 1244
Bsp143I GATC 5 cut(s) 305, 831, 879, 1168, 1190
Bsp1720I GCTNAGC 1 cut(s) 1092
Bsp19I CCATGG 1 cut(s) 1014
BspACI CCGC 2 cut(s) 130, 186
BspANI GGCC 5 cut(s) 125, 176, 752, 767, 794
BspCNI CTCAG 5 cut(s) 40, 139, 1084, 1213, 1243
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 5 cut(s) 184, 205, 285, 740, 1139
BspPI GGATC 4 cut(s) 313, 839, 1163, 1198
BspQI GCTCTTC 2 cut(s) 76, 1249
BspT107I GGYRCC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 295
BsrI ACTGG 1 cut(s) 1104
BssECI CCNNGG 6 cut(s) 450, 469, 747, 872, 1014, 1155
BssMI GATC 5 cut(s) 305, 831, 879, 1168, 1190
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 3 cut(s) 450, 747, 1014
Bst2UI CCWGG 2 cut(s) 340, 471
Bst4CI ACNGT 5 cut(s) 388, 405, 646, 913, 1142
Bst6I CTCTTC 3 cut(s) 76, 382, 1249
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 315, 1240
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 8 cut(s) 126, 416, 458, 549, 700, 1165, 1282, 1291
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 3 cut(s) 41, 331, 568
BstHHI GCGC 4 cut(s) 40, 140, 330, 567
BstKTI GATC 5 cut(s) 308, 834, 882, 1171, 1193
BstMAI GTCTC 2 cut(s) 295, 1112
BstMBI GATC 5 cut(s) 305, 831, 879, 1168, 1190
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 9 cut(s) 37, 92, 122, 173, 224, 230, 983, 1088, 1239
BstNI CCWGG 2 cut(s) 340, 471
BstSCI CCNGG 7 cut(s) 338, 396, 469, 871, 872, 1154, 1155
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 7 cut(s) 67, 70, 211, 286, 382, 937, 1069
BstV2I GAAGAC 3 cut(s) 158, 354, 1251
BstX2I RGATCY 1 cut(s) 1190
BstYI RGATCY 1 cut(s) 1190
BstZI CGGCCG 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 762
BsuRI GGCC 5 cut(s) 125, 176, 752, 767, 794
BtgI CCRYGG 1 cut(s) 1014
BtsCI GGATG 8 cut(s) 126, 416, 458, 549, 700, 1165, 1282, 1291
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 4 cut(s) 642, 740, 1137, 1164
Cac8I GCNNGC 2 cut(s) 315, 1240
CciI TCATGA 1 cut(s) 571
CfoI GCGC 4 cut(s) 40, 140, 330, 567
Cfr13I GGNCC 3 cut(s) 203, 405, 739
Cfr9I CCCGGG 2 cut(s) 872, 1155
CseI GACGC 2 cut(s) 285, 1277
CspCI CAANNNNNGTGG 2 cut(s) 1162, 1197
CviAII CATG 7 cut(s) 520, 572, 743, 817, 931, 1015, 1282
DpnI GATC 5 cut(s) 307, 833, 881, 1170, 1192
DpnII GATC 5 cut(s) 305, 831, 879, 1168, 1190
EaeI YGGCCR 3 cut(s) 123, 174, 792
EagI CGGCCG 1 cut(s) 792
Eam1104I CTCTTC 3 cut(s) 76, 382, 1249
EarI CTCTTC 3 cut(s) 76, 382, 1249
Ecl136II GAGCTC 1 cut(s) 1242
EclXI CGGCCG 1 cut(s) 792
Eco130I CCWWGG 3 cut(s) 450, 747, 1014
Eco24I GRGCYC 1 cut(s) 1244
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 3 cut(s) 203, 405, 739
Eco47III AGCGCT 1 cut(s) 566
Eco52I CGGCCG 1 cut(s) 792
Eco53kI GAGCTC 1 cut(s) 1242
Eco81I CCTNAGG 1 cut(s) 762
Eco88I CYCGRG 3 cut(s) 318, 872, 1155
EcoICRI GAGCTC 1 cut(s) 1242
EcoRII CCWGG 2 cut(s) 338, 469
EcoT14I CCWWGG 3 cut(s) 450, 747, 1014
EcoT38I GRGCYC 1 cut(s) 1244
ErhI CCWWGG 3 cut(s) 450, 747, 1014
FaeI CATG 7 cut(s) 523, 575, 746, 820, 934, 1018, 1285
FalI AAGNNNNNCTT 4 cut(s) 612, 644, 878, 910
FaqI GGGAC 3 cut(s) 189, 752, 1030
FatI CATG 7 cut(s) 519, 571, 742, 816, 930, 1014, 1281
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 1205
Fnu4HI GCNGC 7 cut(s) 81, 84, 225, 275, 371, 951, 1083
FokI GGATG 7 cut(s) 113, 403, 445, 536, 687, 1172, 1289
FriOI GRGCYC 1 cut(s) 1244
Fsp4HI GCNGC 7 cut(s) 81, 84, 225, 275, 371, 951, 1083
FspBI CTAG 2 cut(s) 987, 1212
GlaI GCGC 4 cut(s) 39, 139, 329, 566
GluI GCNGC 7 cut(s) 81, 84, 225, 275, 371, 951, 1083
GsaI CCCAGC 1 cut(s) 831
HaeII RGCGCY 3 cut(s) 41, 331, 568
HaeIII GGCC 5 cut(s) 125, 176, 752, 767, 794
HapII CCGG 6 cut(s) 92, 287, 398, 791, 873, 1156
HgaI GACGC 2 cut(s) 285, 1277
HhaI GCGC 4 cut(s) 40, 140, 330, 567
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 7 cut(s) 523, 575, 746, 820, 934, 1018, 1285
Hin6I GCGC 4 cut(s) 38, 138, 328, 565
HinP1I GCGC 4 cut(s) 38, 138, 328, 565
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 5 cut(s) 14, 98, 592, 1127, 1217
HpaII CCGG 6 cut(s) 92, 287, 398, 791, 873, 1156
HphI GGTGA 3 cut(s) 826, 874, 1277
Hpy166II GTNNAC 2 cut(s) 961, 1206
Hpy188I TCNGA 3 cut(s) 45, 262, 1252
Hpy188III TCNNGA 3 cut(s) 572, 1181, 1274
Hpy8I GTNNAC 2 cut(s) 961, 1206
Hpy99I CGWCG 2 cut(s) 266, 301
HpyAV CCTTC 3 cut(s) 171, 363, 785
HpyCH4III ACNGT 5 cut(s) 388, 405, 646, 913, 1142
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 5 cut(s) 274, 642, 686, 700, 733
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 92, 122, 173, 224, 230, 983, 1088, 1239
HpySE526I ACGT 1 cut(s) 434
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 7 cut(s) 523, 575, 746, 820, 934, 1018, 1285
HspAI GCGC 4 cut(s) 38, 138, 328, 565
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 5 cut(s) 305, 831, 879, 1168, 1190
LguI GCTCTTC 2 cut(s) 76, 1249
LmnI GCTCC 4 cut(s) 248, 256, 289, 1018
Lsp1109I GCAGC 7 cut(s) 67, 70, 211, 286, 382, 937, 1069
LweI GCATC 3 cut(s) 103, 613, 709
MaeI CTAG 2 cut(s) 987, 1212
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 2 cut(s) 907, 1265
MalI GATC 5 cut(s) 307, 833, 881, 1170, 1192
MboI GATC 5 cut(s) 305, 831, 879, 1168, 1190
MboII GAAGA 9 cut(s) 63, 163, 354, 369, 632, 837, 1185, 1236, 1251
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 1 cut(s) 1190
MhlI GDGCHC 1 cut(s) 1244
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 5 cut(s) 269, 663, 972, 1118, 1147
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 2 cut(s) 1136, 1226
MmeI TCCRAC 3 cut(s) 285, 455, 1104
Mox20I TGGCCA 1 cut(s) 176
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 324
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 6 cut(s) 92, 287, 398, 791, 873, 1156
MspR9I CCNGG 7 cut(s) 340, 398, 471, 873, 874, 1156, 1157
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 2 cut(s) 340, 471
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 9 cut(s) 37, 92, 122, 173, 224, 230, 983, 1088, 1239
NciI CCSGG 5 cut(s) 398, 873, 874, 1156, 1157
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 5 cut(s) 305, 831, 879, 1168, 1190
NlaIII CATG 7 cut(s) 523, 575, 746, 820, 934, 1018, 1285
NlaIV GGNNCC 5 cut(s) 184, 205, 285, 740, 1139
NmeAIII GCCGAG 2 cut(s) 101, 272
NmuCI GTSAC 1 cut(s) 1265
PaeR7I CTCGAG 1 cut(s) 318
PagI TCATGA 1 cut(s) 571
PciSI GCTCTTC 2 cut(s) 76, 1249
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PfeI GAWTC 3 cut(s) 14, 98, 592
PflMI CCANNNNNTGG 1 cut(s) 748
PfoI TCCNGGA 1 cut(s) 338
PkrI GCNGC 7 cut(s) 82, 85, 226, 276, 372, 952, 1084
PleI GAGTC 2 cut(s) 1135, 1225
PpsI GAGTC 2 cut(s) 1135, 1225
Psp124BI GAGCTC 1 cut(s) 1244
Psp6I CCWGG 2 cut(s) 338, 469
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 2 cut(s) 338, 469
PspN4I GGNNCC 5 cut(s) 184, 205, 285, 740, 1139
PspPI GGNCC 3 cut(s) 203, 405, 739
PspXI VCTCGAGB 1 cut(s) 318
PsuI RGATCY 1 cut(s) 1190
SacI GAGCTC 1 cut(s) 1244
SapI GCTCTTC 2 cut(s) 76, 1249
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 7 cut(s) 81, 84, 225, 275, 371, 951, 1083
Sau3AI GATC 5 cut(s) 305, 831, 879, 1168, 1190
Sau96I GGNCC 3 cut(s) 203, 405, 739
SchI GAGTC 2 cut(s) 1136, 1226
ScrFI CCNGG 7 cut(s) 340, 398, 471, 873, 874, 1156, 1157
SduI GDGCHC 1 cut(s) 1244
SfaNI GCATC 3 cut(s) 103, 613, 709
Sfr274I CTCGAG 1 cut(s) 318
SinI GGWCC 3 cut(s) 203, 405, 739
SlaI CTCGAG 1 cut(s) 318
SmaI CCCGGG 2 cut(s) 874, 1157
SmlI CTYRAG 4 cut(s) 58, 228, 318, 1109
SmoI CTYRAG 4 cut(s) 58, 228, 318, 1109
Sse9I AATT 5 cut(s) 269, 663, 972, 1118, 1147
SsiI CCGC 2 cut(s) 130, 186
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 987, 1212
SstI GAGCTC 1 cut(s) 1244
StyD4I CCNGG 7 cut(s) 338, 396, 469, 871, 872, 1154, 1155
StyI CCWWGG 3 cut(s) 450, 747, 1014
TaaI ACNGT 5 cut(s) 388, 405, 646, 913, 1142
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 308, 319, 778
TasI AATT 5 cut(s) 269, 663, 972, 1118, 1147
TfiI GAWTC 3 cut(s) 14, 98, 592
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 4 cut(s) 649, 740, 1137, 1171
TseFI GTSAC 1 cut(s) 1265
TseI GCWGC 7 cut(s) 80, 83, 224, 274, 370, 950, 1082
Tsp45I GTSAC 1 cut(s) 1265
TspDTI ATGAA 3 cut(s) 447, 633, 1074
TspMI CCCGGG 2 cut(s) 872, 1155
TspRI CASTG 4 cut(s) 649, 740, 1137, 1171
Van91I CCANNNNNTGG 1 cut(s) 748
VpaK11BI GGWCC 3 cut(s) 203, 405, 739
XhoI CTCGAG 1 cut(s) 318
XmaI CCCGGG 2 cut(s) 872, 1155
XmiI GTMKAC 1 cut(s) 1205
XspI CTAG 2 cut(s) 987, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.