Rh5BG262500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001194
Physical Location & Seq
Reverse (-)
32859764 .. 32860279
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG262500.1

Sequence Viewer

Length: 516 bp
ATGAGTCCAAGGATTAAAGCTTCCTCCAAGGTGAGTAAGAAAAATACCATTGCGCGTACAGTTTGTCGGGGATCACCTTCTGTCACCAAACGTGTTTGTAACCAAAAAGTTAAGTTGTGCTATGAAATCAGCACTTTGAAATGTAACATCATCTGTGAGCGTCCTTTTGAACTAGAGGATGTTGGTAAAGATTTCACTAATATTATTAACAAACGTCAGTGGAATTCCCTGATAGATTATAATGATCCACCAAATGCCACTCTGGTCAAAGAATTCTATGCTCGTCTTGCTGAGGATACTGGTCCTGATCAGGATAAAGTTTATGTCAGGAAAAAGCTGGTACCTTTTAACCCTGAGGTTATTCGATGTACTCTGAATCTTCCTCCTTGTAGTTTACCTGACAGCTATGCCACTTTCAAGAAAATGATTGACAGAAAGAAGCAGCACTTGCCTTCTGACCTTCGTGATTCTGGGACGGTCATGGTGAAATGCCATCATCAAACTATGGATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

19.59

Weight (kDa)

9.36

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_32 PF20167 71 - 132 1.5e-11 Putative plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
Acc65I GGTACC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 340
AccII CGCG 1 cut(s) 55
AclWI GGATC 2 cut(s) 79, 239
AcsI RAATTY 2 cut(s) 223, 272
AfaI GTAC 3 cut(s) 58, 342, 370
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 3 cut(s) 139, 170, 418
AluBI AGCT 3 cut(s) 20, 337, 405
AluI AGCT 3 cut(s) 20, 337, 405
AlwI GGATC 2 cut(s) 79, 239
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 2 cut(s) 223, 272
Asp718I GGTACC 1 cut(s) 340
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 4 cut(s) 43, 66, 76, 496
AvaII GGWCC 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 354
BanI GGYRCC 1 cut(s) 340
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 454
BccI CCATC 1 cut(s) 501
BciVI GTATCC 1 cut(s) 289
BclI TGATCA 1 cut(s) 307
BfaI CTAG 1 cut(s) 173
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 444
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 8, 27
Bse1I ACTGG 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 8, 27
BseGI GGATG 2 cut(s) 184, 514
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 282, 345
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 487
Bsp143I GATC 3 cut(s) 71, 244, 307
BspCNI CTCAG 2 cut(s) 283, 346
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 2 cut(s) 79, 239
BspT107I GGYRCC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 8, 27
BssMI GATC 3 cut(s) 71, 244, 307
BssT1I CCWWGG 2 cut(s) 8, 27
Bst4CI ACNGT 2 cut(s) 61, 478
BstAPI GCANNNNNTGC 1 cut(s) 448
BstDEI CTNAG 2 cut(s) 291, 354
BstF5I GGATG 2 cut(s) 184, 514
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 3 cut(s) 74, 247, 310
BstMBI GATC 3 cut(s) 71, 244, 307
BstMWI GCNNNNNNNGC 2 cut(s) 287, 448
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 454
Bsu36I CCTNAGG 1 cut(s) 354
BsuI GTATCC 1 cut(s) 289
BtsCI GGATG 2 cut(s) 184, 514
BtsIMutI CAGTG 1 cut(s) 224
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 302
CseI GACGC 1 cut(s) 149
Csp6I GTAC 3 cut(s) 57, 341, 369
CviAII CATG 1 cut(s) 481
CviJI RGCY 3 cut(s) 20, 337, 405
CviKI_1 RGCY 3 cut(s) 20, 337, 405
CviQI GTAC 3 cut(s) 57, 341, 369
DdeI CTNAG 2 cut(s) 291, 354
DpnI GATC 3 cut(s) 73, 246, 309
DpnII GATC 3 cut(s) 71, 244, 307
Eco130I CCWWGG 2 cut(s) 8, 27
Eco47I GGWCC 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 354
EcoRI GAATTC 2 cut(s) 223, 272
EcoT14I CCWWGG 2 cut(s) 8, 27
ErhI CCWWGG 2 cut(s) 8, 27
FaeI CATG 1 cut(s) 484
FaiI YATR 7 cut(s) 123, 240, 279, 324, 408, 482, 506
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 487
FatI CATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 443
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 443
HgaI GACGC 1 cut(s) 149
HhaI GCGC 1 cut(s) 55
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 3 cut(s) 4, 376, 467
HphI GGTGA 4 cut(s) 43, 66, 76, 496
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 3 cut(s) 375, 457, 515
Hpy188III TCNNGA 5 cut(s) 305, 311, 328, 418, 464
Hpy8I GTNNAC 1 cut(s) 395
HpyAV CCTTC 3 cut(s) 87, 462, 470
HpyCH4III ACNGT 2 cut(s) 61, 478
HpyCH4IV ACGT 2 cut(s) 91, 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 448
HpyF3I CTNAG 2 cut(s) 291, 354
HpySE526I ACGT 2 cut(s) 91, 214
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 1 cut(s) 53
KpnI GGTACC 1 cut(s) 344
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 71, 244, 307
Lsp1109I GCAGC 1 cut(s) 454
MaeI CTAG 1 cut(s) 173
MaeII ACGT 2 cut(s) 91, 214
MaeIII GTNAC 3 cut(s) 82, 98, 143
MalI GATC 3 cut(s) 73, 246, 309
MboI GATC 3 cut(s) 71, 244, 307
MboII GAAGA 1 cut(s) 371
MluCI AATT 2 cut(s) 223, 272
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 5 cut(s) 34, 169, 286, 349, 393
MseI TTAA 4 cut(s) 15, 111, 207, 348
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 287, 448
NdeII GATC 3 cut(s) 71, 244, 307
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 376, 467
PkrI GCNGC 1 cut(s) 444
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PsiI TTATAA 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 302
RsaI GTAC 3 cut(s) 58, 342, 370
RsaNI GTAC 3 cut(s) 57, 341, 369
SaqAI TTAA 4 cut(s) 15, 111, 207, 348
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 3 cut(s) 71, 244, 307
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 13
SinI GGWCC 1 cut(s) 302
Sse9I AATT 2 cut(s) 223, 272
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 173
StyI CCWWGG 2 cut(s) 8, 27
TaaI ACNGT 2 cut(s) 61, 478
TaiI ACGT 2 cut(s) 94, 217
TaqI TCGA 1 cut(s) 364
TasI AATT 2 cut(s) 223, 272
TatI WGTACW 1 cut(s) 368
TfiI GAWTC 2 cut(s) 376, 467
Tru1I TTAA 4 cut(s) 15, 111, 207, 348
Tru9I TTAA 4 cut(s) 15, 111, 207, 348
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 1 cut(s) 442
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 138
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 2 cut(s) 223, 272
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.