Rh5BG212600

function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001194
Physical Location & Seq
Forward (+)
24643655 .. 24644932
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG212600.1

Sequence Viewer

Length: 1278 bp
ATGCCTCCCTCTAAGCCTGCTCTGCGCTCTCGGAAGCATCAAGTTGTTTCTCCTGCTTCCTCTCAAGATGCTACCTTGGCTGCGCCATCTTCTTCAAGACCTGCTGCCTCCAGTAAGCACCCCTCAAAGAAGACCAAAAGAGTTCAAGAATCTTCTCCAGATGCCCCATCTTCCACTTTGGACTCCTTTCGATTTGCATCTGCTGACTCTCAATTCAGGTTTTATTCCTTCTCTTCTCACAGACCGGTTCTTTTTGAGAAGAAATTTGATCTTCCTAGTCTTGGTCCTGCATTCTCTGACATTTTGGCTTATTATGGTTGGTTGGATTTGCTTTCTGGGTTATCTGATGCAAACCTTTCTATTGTCCGAGAGTTTTATGCTAGTTTTCCATCCCATATTGATTCTGCTGCTCCTTCTTCGGAACCGGTTTTCAATGCTCATCTGTATCAAGTATTTGTTCGTGGACAGTGGGTTGATTTCAATCCATCTCACATTCATTCCACGCTTCATCTCTCTGAGCTCAACTCTGATGTCTCGATTGACTCTGTTCAAAGCTATATTGAAGGATTCAACAAAAAATGGCTCATGGATGAGTTGTATTGGAATAAGGAGGCAGTCAATCCATCTGACACCTATGCTACTCTTGGTCATTTGTCTCAGCTCACCAAGCTTGTATATCATTTTGTGACCAGTTACTTGTGGCCGTCTTCTCAGTCTTCTCAACTCTCCATCGGACCGGGTGCTTTAGCTGTTGCTATTAAGACTAGAAAGTTTTCCTCCATTCCTCTTCATCAATATGTCTTTGATAGAGTGAAGGAAGCTGCTTTAACCAAGGGAACCAGTACCTCAGCCTTCCTTATTTTTCCATGTCTGATCACTCAAATCTGCAATGCAAGCAATGTTCCCACCTCTCGTGATGATGATTGGGCTGAGGCCCCCTTGTATAATCTGCATTCTCTGTCTCGCAGTGATGGGCAAAGCTCTCTTCCTTGCCCCTCCATCAATGATGAAGCTTGGTACACGCTATTGGATCAGCGTTTTTCTCGTCTTGAAGCATCATTTACTGCACTTCAGTCCTCCATCAAAGAGACCCTGGCTCCGTTTTCTCGTCTCGTTGATGCTCATGAGCAGGCCATTCTGAATGCCCTCTCTGCTGCTGCTCCTTCAGCCCCTGCTGCTTCTGATAGTGAAGAAACTGAATCTCCATCCGCCTTAGCTTTTGTCCACAGTGAGCGTCGCTCCAAGCGCTTTGCTGACCGCCCTGCTGCTCATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

46.87

Weight (kDa)

6.46

Isoelectric Point (pI)

65.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_32 PF20167 105 - 302 7.1e-24 Putative plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AciI CCGC 2 cut(s) 1209, 1258
AclWI GGATC 1 cut(s) 1038
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 2 cut(s) 1055, 1149
AfaI GTAC 2 cut(s) 844, 1019
AfeI AGCGCT 1 cut(s) 1247
AfiI CCNNNNNNNGG 1 cut(s) 281
AgeI ACCGGT 2 cut(s) 244, 424
AgsI TTSAA 8 cut(s) 96, 146, 433, 481, 551, 563, 571, 1052
AjnI CCWGG 1 cut(s) 1092
AluBI AGCT 9 cut(s) 520, 555, 661, 670, 749, 821, 981, 1013, 1217
AluI AGCT 9 cut(s) 520, 555, 661, 670, 749, 821, 981, 1013, 1217
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 5 cut(s) 538, 660, 966, 1082, 1115
AlwI GGATC 1 cut(s) 1038
AlwNI CAGNNNCTG 1 cut(s) 1172
Aor51HI AGCGCT 1 cut(s) 1247
AoxI GGCC 3 cut(s) 701, 933, 1131
ApeKI GCWGC 8 cut(s) 80, 104, 407, 821, 1154, 1157, 1175, 1265
ApoI RAATTY 1 cut(s) 263
AsiGI ACCGGT 2 cut(s) 244, 424
Asp700I GAANNNNTTC 1 cut(s) 772
AspLEI GCGC 3 cut(s) 27, 85, 1248
AspS9I GGNCC 3 cut(s) 284, 734, 934
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 2 cut(s) 284, 734
BanII GRGCYC 1 cut(s) 522
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 3 cut(s) 137, 699, 708
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 2 cut(s) 847, 930
BbvI GCAGC 8 cut(s) 67, 91, 394, 808, 1141, 1144, 1162, 1252
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 1 cut(s) 1094
BclI TGATCA 1 cut(s) 873
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 5 cut(s) 538, 660, 966, 1082, 1115
BfaI CTAG 4 cut(s) 276, 381, 765, 1276
BfoI RGCGCY 1 cut(s) 1249
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 8 cut(s) 81, 105, 408, 822, 1155, 1158, 1176, 1266
BlsI GCNGC 8 cut(s) 82, 106, 409, 823, 1156, 1159, 1177, 1267
Bme1390I CCNGG 2 cut(s) 738, 1094
Bme18I GGWCC 2 cut(s) 284, 734
BmgT120I GGNCC 3 cut(s) 284, 734, 934
BmiI GGNNCC 4 cut(s) 423, 838, 936, 1098
BmrFI CCNGG 2 cut(s) 738, 1094
BmsI GCATC 7 cut(s) 46, 58, 151, 206, 337, 1064, 1108
BpiI GAAGAC 3 cut(s) 137, 699, 708
BplI GAGNNNNNCTC 4 cut(s) 509, 541, 1223, 1255
BpmI CTGGAG 2 cut(s) 94, 141
Bpu10I CCTNAGC 3 cut(s) 847, 930, 1213
BpuEI CTTGAG 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 738
BsaBI GATNNNNATC 2 cut(s) 196, 480
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 3 cut(s) 75, 831, 1092
BsaWI WCCGGW 2 cut(s) 244, 424
BsaXI ACNNNNNCTCC 4 cut(s) 1081, 1111, 1186, 1216
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse118I RCCGGY 2 cut(s) 244, 424
Bse1I ACTGG 3 cut(s) 111, 690, 840
Bse3DI GCAATG 2 cut(s) 895, 904
Bse8I GATNNNNATC 2 cut(s) 196, 480
BseBI CCWGG 1 cut(s) 1094
BseDI CCNNGG 3 cut(s) 75, 831, 1092
BseGI GGATG 3 cut(s) 389, 595, 1205
BseJI GATNNNNATC 2 cut(s) 196, 480
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 2 cut(s) 895, 904
BseMII CTCAG 5 cut(s) 507, 671, 725, 861, 921
BseNI ACTGG 3 cut(s) 111, 690, 840
BseXI GCAGC 8 cut(s) 67, 91, 394, 808, 1141, 1144, 1162, 1252
BsgI GTGCAG 1 cut(s) 1050
BshFI GGCC 3 cut(s) 703, 935, 1133
BshTI ACCGGT 2 cut(s) 244, 424
BsiHKAI GWGCWC 1 cut(s) 522
BsiSI CCGG 3 cut(s) 245, 425, 737
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 5 cut(s) 538, 660, 966, 1082, 1115
BsmBI CGTCTC 1 cut(s) 1115
BsmI GAATGC 3 cut(s) 290, 952, 1147
BsnI GGCC 3 cut(s) 703, 935, 1133
Bso31I GGTCTC 1 cut(s) 1082
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 3 cut(s) 268, 873, 1030
BspACI CCGC 2 cut(s) 1209, 1258
BspANI GGCC 3 cut(s) 703, 935, 1133
BspCNI CTCAG 5 cut(s) 508, 670, 724, 860, 922
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 4 cut(s) 423, 838, 936, 1098
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 1038
BspTNI GGTCTC 1 cut(s) 1082
BsrDI GCAATG 2 cut(s) 895, 904
BsrFI RCCGGY 2 cut(s) 244, 424
BsrI ACTGG 3 cut(s) 111, 690, 840
BssAI RCCGGY 2 cut(s) 244, 424
BssECI CCNNGG 3 cut(s) 75, 831, 1092
BssMI GATC 3 cut(s) 268, 873, 1030
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 2 cut(s) 75, 831
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 1 cut(s) 1094
Bst4CI ACNGT 2 cut(s) 468, 1229
Bst6I CTCTTC 3 cut(s) 238, 792, 990
BstC8I GCNNGC 3 cut(s) 18, 895, 1131
BstDEI CTNAG 7 cut(s) 12, 516, 657, 711, 847, 930, 1213
BstF5I GGATG 3 cut(s) 389, 595, 1205
BstH2I RGCGCY 1 cut(s) 1249
BstHHI GCGC 3 cut(s) 27, 85, 1248
BstKTI GATC 3 cut(s) 271, 876, 1033
BstMAI GTCTC 5 cut(s) 538, 660, 966, 1082, 1115
BstMBI GATC 3 cut(s) 268, 873, 1030
BstMWI GCNNNNNNNGC 8 cut(s) 22, 77, 667, 894, 1151, 1166, 1175, 1245
BstNI CCWGG 1 cut(s) 1094
BstSCI CCNGG 2 cut(s) 736, 1092
BstV1I GCAGC 8 cut(s) 67, 91, 394, 808, 1141, 1144, 1162, 1252
BstV2I GAAGAC 3 cut(s) 137, 699, 708
BsuRI GGCC 3 cut(s) 703, 935, 1133
BtsCI GGATG 3 cut(s) 389, 595, 1205
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 3 cut(s) 473, 973, 1234
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 18, 895, 1131
CaiI CAGNNNCTG 1 cut(s) 1172
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 3 cut(s) 27, 85, 1248
Cfr10I RCCGGY 2 cut(s) 244, 424
Cfr13I GGNCC 3 cut(s) 284, 734, 934
CpoI CGGWCCG 1 cut(s) 734
CseI GACGC 1 cut(s) 1223
Csp6I GTAC 2 cut(s) 843, 1018
CspAI ACCGGT 2 cut(s) 244, 424
CspI CGGWCCG 1 cut(s) 734
CviAII CATG 3 cut(s) 586, 867, 1124
CviQI GTAC 2 cut(s) 843, 1018
DdeI CTNAG 7 cut(s) 12, 516, 657, 711, 847, 930, 1213
DpnI GATC 3 cut(s) 270, 875, 1032
DpnII GATC 3 cut(s) 268, 873, 1030
EaeI YGGCCR 1 cut(s) 701
Eam1104I CTCTTC 3 cut(s) 238, 792, 990
EarI CTCTTC 3 cut(s) 238, 792, 990
EciI GGCGGA 1 cut(s) 1198
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 75, 831
Eco24I GRGCYC 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 1082
Eco47I GGWCC 2 cut(s) 284, 734
Eco47III AGCGCT 1 cut(s) 1247
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 2 cut(s) 1055, 1149
EcoICRI GAGCTC 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 934
EcoRII CCWGG 1 cut(s) 1092
EcoT14I CCWWGG 2 cut(s) 75, 831
EcoT38I GRGCYC 1 cut(s) 522
ErhI CCWWGG 2 cut(s) 75, 831
Esp3I CGTCTC 1 cut(s) 1115
FaeI CATG 3 cut(s) 589, 870, 1127
FatI CATG 3 cut(s) 585, 866, 1123
FbaI TGATCA 1 cut(s) 873
Fnu4HI GCNGC 8 cut(s) 81, 105, 408, 822, 1155, 1158, 1176, 1266
FokI GGATG 3 cut(s) 376, 602, 1192
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 8 cut(s) 81, 105, 408, 822, 1155, 1158, 1176, 1266
FspBI CTAG 4 cut(s) 276, 381, 765, 1276
GlaI GCGC 3 cut(s) 26, 84, 1247
GluI GCNGC 8 cut(s) 81, 105, 408, 822, 1155, 1158, 1176, 1266
GsuI CTGGAG 2 cut(s) 94, 141
HaeII RGCGCY 1 cut(s) 1249
HaeIII GGCC 3 cut(s) 703, 935, 1133
HapII CCGG 3 cut(s) 245, 425, 737
HgaI GACGC 1 cut(s) 1223
HhaI GCGC 3 cut(s) 27, 85, 1248
Hin1II CATG 3 cut(s) 589, 870, 1127
Hin6I GCGC 3 cut(s) 25, 83, 1246
HinP1I GCGC 3 cut(s) 25, 83, 1246
HindIII AAGCTT 2 cut(s) 668, 1011
HinfI GANTC 7 cut(s) 149, 182, 206, 401, 542, 567, 1199
HpaII CCGG 3 cut(s) 245, 425, 737
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 3 cut(s) 464, 1020, 1225
Hpy188III TCNNGA 8 cut(s) 65, 96, 146, 158, 535, 914, 1049, 1124
Hpy8I GTNNAC 3 cut(s) 464, 1020, 1225
Hpy99I CGWCG 1 cut(s) 1239
HpyAV CCTTC 6 cut(s) 238, 423, 557, 808, 862, 1173
HpyCH4III ACNGT 2 cut(s) 468, 1229
HpyCH4V TGCA 7 cut(s) 197, 290, 350, 888, 893, 952, 1067
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 77, 667, 894, 1151, 1166, 1175, 1245
HpyF3I CTNAG 7 cut(s) 12, 516, 657, 711, 847, 930, 1213
Hsp92II CATG 3 cut(s) 589, 870, 1127
HspAI GCGC 3 cut(s) 25, 83, 1246
Ksp22I TGATCA 1 cut(s) 873
Kzo9I GATC 3 cut(s) 268, 873, 1030
LmnI GCTCC 4 cut(s) 415, 1102, 1165, 1244
Lsp1109I GCAGC 8 cut(s) 67, 91, 394, 808, 1141, 1144, 1162, 1252
LweI GCATC 7 cut(s) 46, 58, 151, 206, 337, 1064, 1108
MaeI CTAG 4 cut(s) 276, 381, 765, 1276
MaeIII GTNAC 2 cut(s) 685, 692
MalI GATC 3 cut(s) 270, 875, 1032
MboI GATC 3 cut(s) 268, 873, 1030
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 2 cut(s) 212, 263
MlyI GAGTC 3 cut(s) 176, 200, 536
MmeI TCCRAC 1 cut(s) 303
MroXI GAANNNNTTC 1 cut(s) 772
MseI TTAA 2 cut(s) 759, 827
MslI CAYNNNNRTG 1 cut(s) 795
MspI CCGG 3 cut(s) 245, 425, 737
MspR9I CCNGG 2 cut(s) 738, 1094
Mva1269I GAATGC 3 cut(s) 290, 952, 1147
MvaI CCWGG 1 cut(s) 1094
MwoI GCNNNNNNNGC 8 cut(s) 22, 77, 667, 894, 1151, 1166, 1175, 1245
NciI CCSGG 1 cut(s) 738
NdeII GATC 3 cut(s) 268, 873, 1030
NlaIII CATG 3 cut(s) 589, 870, 1127
NlaIV GGNNCC 4 cut(s) 423, 838, 936, 1098
NmuCI GTSAC 1 cut(s) 685
PagI TCATGA 1 cut(s) 1123
PctI GAATGC 3 cut(s) 290, 952, 1147
PdmI GAANNNNTTC 1 cut(s) 772
PfeI GAWTC 4 cut(s) 149, 401, 567, 1199
PinAI ACCGGT 2 cut(s) 244, 424
PkrI GCNGC 8 cut(s) 82, 106, 409, 823, 1156, 1159, 1177, 1267
PleI GAGTC 3 cut(s) 176, 200, 536
PpsI GAGTC 3 cut(s) 176, 200, 536
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 1092
PspGI CCWGG 1 cut(s) 1092
PspN4I GGNNCC 4 cut(s) 423, 838, 936, 1098
PspPI GGNCC 3 cut(s) 284, 734, 934
PstNI CAGNNNCTG 1 cut(s) 1172
RsaI GTAC 2 cut(s) 844, 1019
RsaNI GTAC 2 cut(s) 843, 1018
RseI CAYNNNNRTG 1 cut(s) 795
Rsr2I CGGWCCG 1 cut(s) 734
RsrII CGGWCCG 1 cut(s) 734
SacI GAGCTC 1 cut(s) 522
SaqAI TTAA 2 cut(s) 759, 827
SatI GCNGC 8 cut(s) 81, 105, 408, 822, 1155, 1158, 1176, 1266
Sau3AI GATC 3 cut(s) 268, 873, 1030
Sau96I GGNCC 3 cut(s) 284, 734, 934
SchI GAGTC 3 cut(s) 176, 200, 536
ScrFI CCNGG 2 cut(s) 738, 1094
SduI GDGCHC 1 cut(s) 522
SfaNI GCATC 7 cut(s) 46, 58, 151, 206, 337, 1064, 1108
SinI GGWCC 2 cut(s) 284, 734
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 2 cut(s) 212, 263
SsiI CCGC 2 cut(s) 1209, 1258
SspMI CTAG 4 cut(s) 276, 381, 765, 1276
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 736, 1092
StyI CCWWGG 2 cut(s) 75, 831
TaaI ACNGT 2 cut(s) 468, 1229
TaqI TCGA 2 cut(s) 190, 536
TasI AATT 2 cut(s) 212, 263
TfiI GAWTC 4 cut(s) 149, 401, 567, 1199
Tru1I TTAA 2 cut(s) 759, 827
Tru9I TTAA 2 cut(s) 759, 827
TscAI CASTG 3 cut(s) 473, 973, 1234
TseFI GTSAC 1 cut(s) 685
TseI GCWGC 8 cut(s) 80, 104, 407, 821, 1154, 1157, 1175, 1265
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 4 cut(s) 485, 497, 779, 1023
TspGWI ACGGA 1 cut(s) 1089
TspRI CASTG 3 cut(s) 473, 973, 1234
VpaK11BI GGWCC 2 cut(s) 284, 734
XapI RAATTY 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 772
XspI CTAG 4 cut(s) 276, 381, 765, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.