FvH4_5g19751

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001194
Physical Location & Seq
Forward (+)
11585421 .. 11586936
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19751.t1

Sequence Viewer

Length: 486 bp
ATGGCACACTCGTCAGAAAACATTCCTCTACCGTCGTCAGCTATTGGAGGAATATTGGAGATATGGCTGTGGTATGCAAGTTATTCGAGGAATATTGGAGCCAAAGCAGCAACCAAGACCATCAAAATCTTCTCGGGTCCTCGGCTAGTTCTTCAAGGTTGGCGATTTAACTCTCTCTTTTGGTACCCAATGCATGCCGCTTCTTCTACTTCTATCTCTGTGCTCATTGGATCAGAGTCTAAGGCCCAGTTGGATTTTTGCGTTGATGAAGAGACCGTTCCAATCGAGCTCTCGGGTTCCTCTAGCTCTGACCTTGAATTCACAGAGCCTTCTCGAAAGACCACTCGGAGCTCTAGCAAGGCCCTTGCTGGTTCCTCCAAATCTGGGTCCAAAGAGTCACTGAGTCTTGAAAGAGTTGGTTTCGTATTTTCAGGCTCAGGGTTTACTGAGTCCTTGTTTACTTGGTCATTAGTCAATAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.42

Weight (kDa)

7.86

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 183
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 238
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 3 cut(s) 155, 317, 410
AluBI AGCT 4 cut(s) 41, 289, 306, 351
AluI AGCT 4 cut(s) 41, 289, 306, 351
Alw21I GWGCWC 3 cut(s) 225, 291, 353
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 238
Ama87I CYCGRG 2 cut(s) 133, 292
AoxI GGCC 2 cut(s) 243, 360
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 21
Asp718I GGTACC 1 cut(s) 183
AspS9I GGNCC 4 cut(s) 137, 244, 361, 387
AvaI CYCGRG 2 cut(s) 133, 292
AvaII GGWCC 2 cut(s) 137, 387
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 2 cut(s) 291, 353
Bbv12I GWGCWC 3 cut(s) 225, 291, 353
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 4 cut(s) 146, 303, 354, 484
BisI GCNGC 2 cut(s) 108, 198
BlsI GCNGC 2 cut(s) 109, 199
Bme18I GGWCC 2 cut(s) 137, 387
BmeT110I CYCGRG 2 cut(s) 133, 292
BmgT120I GGNCC 4 cut(s) 137, 244, 361, 387
BmiI GGNNCC 6 cut(s) 100, 138, 185, 298, 373, 388
BmrI ACTGGG 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 241
Bpu10I CCTNAGC 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMII CTCAG 3 cut(s) 392, 438, 450
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 2 cut(s) 245, 362
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 3 cut(s) 225, 291, 353
BsiHKCI CYCGRG 2 cut(s) 133, 292
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 1 cut(s) 266
BsnI GGCC 2 cut(s) 245, 362
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 2 cut(s) 133, 292
Bsp1286I GDGCHC 3 cut(s) 225, 291, 353
Bsp143I GATC 1 cut(s) 230
BspACI CCGC 1 cut(s) 198
BspANI GGCC 2 cut(s) 245, 362
BspCNI CTCAG 3 cut(s) 393, 439, 449
BspLI GGNNCC 6 cut(s) 100, 138, 185, 298, 373, 388
BspPI GGATC 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 266
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 33, 277
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 4 cut(s) 240, 401, 436, 447
BstKTI GATC 1 cut(s) 233
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 119
BsuRI GGCC 2 cut(s) 245, 362
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 137, 244, 361, 387
Csp6I GTAC 1 cut(s) 184
CviAII CATG 1 cut(s) 194
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 4 cut(s) 240, 401, 436, 447
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Ecl136II GAGCTC 2 cut(s) 289, 351
Eco24I GRGCYC 2 cut(s) 291, 353
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 2 cut(s) 137, 387
Eco53kI GAGCTC 2 cut(s) 289, 351
Eco88I CYCGRG 2 cut(s) 133, 292
EcoICRI GAGCTC 2 cut(s) 289, 351
EcoO109I RGGNCCY 2 cut(s) 137, 361
EcoRI GAATTC 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 195
EcoT38I GRGCYC 2 cut(s) 291, 353
FaeI CATG 1 cut(s) 197
FaiI YATR 3 cut(s) 64, 75, 195
FatI CATG 1 cut(s) 193
Fnu4HI GCNGC 2 cut(s) 108, 198
FriOI GRGCYC 2 cut(s) 291, 353
Fsp4HI GCNGC 2 cut(s) 108, 198
FspBI CTAG 4 cut(s) 146, 303, 354, 484
GluI GCNGC 2 cut(s) 108, 198
HaeIII GGCC 2 cut(s) 245, 362
Hin1II CATG 1 cut(s) 197
HinfI GANTC 4 cut(s) 236, 395, 403, 449
Hpy166II GTNNAC 2 cut(s) 444, 459
Hpy188I TCNGA 4 cut(s) 16, 235, 310, 348
Hpy188III TCNNGA 2 cut(s) 333, 407
Hpy8I GTNNAC 2 cut(s) 444, 459
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 339
HpyCH4III ACNGT 2 cut(s) 33, 277
HpyCH4V TGCA 2 cut(s) 77, 193
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 4 cut(s) 240, 401, 436, 447
Hsp92II CATG 1 cut(s) 197
KpnI GGTACC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 230
LmnI GCTCC 2 cut(s) 98, 348
LpnPI CCDG 5 cut(s) 260, 354, 369, 417, 423
Lsp1109I GCAGC 1 cut(s) 119
MaeI CTAG 4 cut(s) 146, 303, 354, 484
MaeIII GTNAC 1 cut(s) 396
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 4 cut(s) 121, 143, 195, 281
MhlI GDGCHC 3 cut(s) 225, 291, 353
MluCI AATT 1 cut(s) 317
MlyI GAGTC 4 cut(s) 245, 404, 412, 458
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 6 cut(s) 36, 41, 81, 150, 310, 385
Mph1103I ATGCAT 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 1 cut(s) 230
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 6 cut(s) 100, 138, 185, 298, 373, 388
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 195
NspI RCATGY 1 cut(s) 197
PaeI GCATGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 21
PkrI GCNGC 2 cut(s) 109, 199
PleI GAGTC 4 cut(s) 244, 403, 411, 457
PpsI GAGTC 4 cut(s) 244, 403, 411, 457
PpuMI RGGWCCY 1 cut(s) 137
Psp124BI GAGCTC 2 cut(s) 291, 353
Psp5II RGGWCCY 1 cut(s) 137
PspN4I GGNNCC 6 cut(s) 100, 138, 185, 298, 373, 388
PspPI GGNCC 4 cut(s) 137, 244, 361, 387
PspPPI RGGWCCY 1 cut(s) 137
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SacI GAGCTC 2 cut(s) 291, 353
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 108, 198
Sau3AI GATC 1 cut(s) 230
Sau96I GGNCC 4 cut(s) 137, 244, 361, 387
SchI GAGTC 4 cut(s) 245, 404, 412, 458
SduI GDGCHC 3 cut(s) 225, 291, 353
SetI ASST 6 cut(s) 43, 160, 291, 308, 315, 353
SinI GGWCC 2 cut(s) 137, 387
SphI GCATGC 1 cut(s) 197
Sse9I AATT 1 cut(s) 317
SsiI CCGC 1 cut(s) 198
SspI AATATT 2 cut(s) 54, 94
SspMI CTAG 4 cut(s) 146, 303, 354, 484
SstI GAGCTC 2 cut(s) 291, 353
TaaI ACNGT 2 cut(s) 33, 277
TaqI TCGA 3 cut(s) 86, 285, 334
TasI AATT 1 cut(s) 317
TauI GCSGC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 405
TseFI GTSAC 1 cut(s) 396
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 1 cut(s) 282
TspRI CASTG 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 137, 387
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 4 cut(s) 146, 303, 354, 484
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.