Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Forward (+)

33904798 .. 33913337

Gene Structure 8540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g40090.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3963 bp

ATGGGGAAGCAGAAGGGAGACGGAGCTGGGAGCAAGGCCCGCCCTTCCAGTAGCAGCTCTGGTGATAGCAATGCATTTATCTTGCCAAATAAGAGAAACAGGAAAAGGAAGGGCACCACTCAGGAGGGTAAGATACTTGGCACAAGAAAATCACAGAAGCTGACCAAATCCCAAGAAAAGAAGTTGCACAAGTTGGAGGAGGATAAGGAAAAAGCCCTTTTGCTATCCAAGAGCATTGAGGCTTTAGGGAAGTATAAGCTTCCGGAGGGTGTACATTCTCTTTTGCAGTCATCAAAAGATATTGGTCAGGTTGAATCAAAAAAGGAAAGACGTAGGAAGGCTGTTCTATTCTCTAAAGCAAGATTAGAAGTTCCGTATACTGACCAACCATTTAAAAAGAAAGTCGATGTCGATGCTTGTAGTGAAAGTGAACCTGAGTCAGAAAGAACTCGATCAAGGCAGGATCTTGACAAAAGTGGTCAAGTGCAATCGATGGTAATACAAAAAGAAATCCATAAGACCGCATCTGTATGTTTGAACTCCTCGCAGGGGATAGTCTCTAGCAGAGGACATGTCACAGATGGTGGTCCTGCTGCCAGCTCATCATCTAAAATTGTTATCTGCAAGGAACATGATGTGTCTTTGCCAGAATACGTAACTCCCAATGAGAATGATGACCATGAAAGAACAGAGTCAATGGATAGGGTAAAAGGGAGCCCAAAAGTAACTTCAAGCCGGACAAGTGAAGTTTCAGATTTTGCTGAGCCACGGTCTCTTATTGCTCCAACTATAGTGAATGTATCAAGGCCCGTTGAAGTTGAAAACACAAGGAAGGATCTTCCAATAGTAATGATGGAACAGGAGATAATGGAGGCTGTTAATGACCATTCTACTGTTATTATATGTGGAGAGACAGGGTGTGGTAAAACCACTCAGGTTCCCCAGTTTCTTTTTGAAGCTGGCTATGGTTCAAGCCACTCTTGTCATCGAAGCGGGATAATTGGTGTCACTCAACCTCGTCGTGTTGCAGTCCTTGCAACTGCTAAGCGAGTTGCATATGAGCTTGGTCTACATTTGGGTAAAGAGGTTGGCTTTCAAGTTAGATTTGACAAAAAAATTGGCGAAAGTTCCTCCATCAAGTTCATGACAGATGGAATATTACTACGCGAGCTTCAGAGTGATTTTTTGTTGATGCGATATTCTGTAATAATTCTTGATGAAGCTCATGAGCGGAGCTTGAGCACGGATATACTTATTGGAATGCTTTCTCGTGTAATTAGTACTCGCCAGGAAATATATGCTAAGCAGCTGCTTTCTGGAAAATCTATAGATCCTAGACAACAAGTTTTTCCGTTAAAGCTTGTGCTGATGAGTGCCACAATGCGAGTGGAAGACTTCATTTCTGGTAGAAAGTTGTTTCGTGAAATCCCGCCAGTCATAGAAGTTCCCACCAGACAATTTCCAGTAACCACTCACTTCTCAAAAAAAACTGGGACGGACTATATTGACCAAGCATATAAAAAAGTCTTGGCAATTCACAAGAGGCTGCCCCGTGGGGGCATACTTGTCTTCGTCACTGGGCAGAGGGAAGTAGAGTCCTTGTGTCGAAAACTACGTAGAGCTTCAGCAGAACTGGTCATGAAAACATCTGGAAGGAAAATTGAGTATAATACCCATGATGCCTCTAAAATAAATGTTGAACTACTGGATATGAATGAGATTAATGAAGCATTTGAGGTTGATGAAAATTCAGCCGACCGTCAAGTTGACAGGTTTAGCTCCATTGATGAAGATCGGGGTAATATAACTGAGGATGAATTGGATGCTTCGTATGATTCAGAAACAGAAACAGAGAGTGAAGTGGAAATTGATGATGATGGGGACTTATTGTTACACGATACCACAGAAATTGATGGTGTTGGTGCAGATGTTTTAGGAGAGACGGGGAGCATTGCTTCACTGAAAGCTGCTTTTGAAGTTTTGGCCAGTAAAACTTCTGATGGGAAACAGCCCAGTTCAGTTACACTAGATGCTTGCTGTAATAAATTCCGTCCTATTCTTGGGAAAAAGAGTGAGATTGATATTCACAATTCTCCTGGTGGCTTGCATGTTCTCCCTCTCTATGCCATGCTGCCTGCGGCTTCTCAGCTTCGCGTATTTGAAGAAGTCAAGGAAGGAGATCGACTAGTAGTTGTTGCCACAAATGTGGCTGAGACCTCTTTAACTATACCTGGGATAAAGTATGTCGTTGATACTGGCAGAGTAAAAGTGAAAAACTACAATTCCTCAAATGGTATGGAAACATATAACATAGAGTGGATAAGTAAGGCATCAGCTGCTCAACGTAAAGGAAGAGCTGGAAGAACAGGACCCGGACATTGTTATCGTCTTTATTCTTCTGCCGTATATAATAATGAATTTCCTGACTTTTCTCCAGCTGAAATATTGAAGGTCCCTATTGATGGTGTTGTCCTTCTAATGAAATCTATGAATATTGAAAAGGTATCCAATTTTCCATTTCCAACTCCACCTGATGTTGCTGCCTTGGGTGAAGCAGAACGTTGCTTGAAGGCGCTTGAAGCACTTGACAGCAATGGCAGATTAACACCCGTGGGGAAGGCCATGGCTCGTTACCCCATGAGTCCTCGCCACTCAAGGATGCTCCTTACGGTTATTCAAATCATGTACGAGGAGAAAAGTTATCCCCGCAGTAAATTAGTGTTAGCATATGCAGTTGCAGCAGCGGCTGCATTGAGTTTATCAAATCCTTTTGTGAGGCAGTTTGAAAATGACTCAAAAACTCATGACTTGGATCAGGATGAAAATCCCGGCGCTCCTGTGAACAAAATGGTTTCAGAGATGCAAGAAAAGTTGAGAAGGAAAAAACTAAAAGAAACTATAAAGGTGTTTCGGGAAAAATTTTCTAATCCTATCAGTGATGCTCTGACTGTAGCTTATGCTTTGCAGTGCTATGAACTTTCGAGGAGCCCAGTAAAGTTCTGCAATGACAATGCCCTACATCCGAAAACTATGGAGGAAATGTCCAAGCTGAGAAAGCAACTGCTCCAACTTGTGTTTAATCAATGTGGTGCATCTGGCAATGAGAAGGGTTTTTCATGCATTTTTGGAAATCTGGAAGAAGTTGAACATGTTTGGAGGGTTTCCCATGATAAAAGTCCTCTTTCATTGTATGAGGAAGACCTCTTAGGCCAAGCTATATGTGCTGGTTGGGCAGATAGGGTGGCGAAACGCATTAAAGGAACCTCAGGGTCATCTGAAGTAGATAGAAAAGTTCACGCCGTGCGGTATCAAGCCTGCATGGTTGAAGAGACTGTGTTCCTCCACCGTTGGTCAGCTGTTTCTAATGCTGCCCCCGAATTTTTGGTTTACACTGAGCTAATACAGACAAGACGTCCATACATGCATGGTGTAACTAGTGTAAAACCAGAATGGCTTGTTAAGTATGCTCCGTCTTTATGCACTTTCTCGGCAACCTCAACTGATGCGAAACAGTATTATGAGCCTGTGACTGACCGAGTGTTGCATGATGTCATTCCAGCCTTTGGTCCTCGTCTATGGAAGCTTCCCCCACATAGTTTACCTATCACTAATTATGTCTCTCGAGTGACAATTTTCGCTTATGCGTTGCTTGAAGGTCAGGTTTTGCCATGCTTGAAACATGCTCGTGAGTTTATGAAGGCACCTCCAGCAAGTATTTTACGGCCAGAGGCACAAGGCCAAAGACGGGTTGGGAATTTGTTAGCCAAGTTGAACACCAAAAAGATAGACAGTTGTTCTATGTTAAGAGAGGTCTGGAAGGAGAATCCTGATGAATTGCGTTCAGAAATCCAGGACTGGTTTAAGGAGAGTTTCCACAAGAATTTGGCAACTCTGTGGTCAGTCATGCTTAGGGAAGTTGTTTTAGAGCTGGGAGAACGGTTTTCAAAGAGACAGAAAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1321

Amino Acids

147.56

Weight (kDa)

8.65

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	695 - 787	4.3e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	849 - 876	2.9e-09	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	878 - 980	2.2e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	1067 - 1155	9.9e-19	Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C	PF23362	1222 - 1298	5.8e-19	DEAH helicase DHX37 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3415
AccB1I	GGYRCC	2 cut(s)	113, 3700
AccB7I	CCANNNNNTGG	1 cut(s)	3563
AccBSI	CCGCTC	1 cut(s)	1231
AccI	GTMKAC	2 cut(s)	377, 1069
AccII	CGCG	2 cut(s)	1167, 2154
AccIII	TCCGGA	1 cut(s)	262
AciI	CCGC	9 cut(s)	40, 522, 993, 1231, 1430, 2138, 2707, 2744, 3304
AclI	AACGTT	1 cut(s)	2560
AclWI	GGATC	4 cut(s)	471, 843, 1325, 2820
AcoI	YGGCCR	2 cut(s)	1983, 3722
AcsI	RAATTY	7 cut(s)	1747, 2045, 2417, 2918, 3377, 3754, 3880
AcuI	CTGAAG	3 cut(s)	1157, 1608, 3297
AcyI	GRCGYC	1 cut(s)	3412
AdeI	CACNNNGTG	1 cut(s)	3301
AfaI	GTAC	3 cut(s)	273, 1282, 2687
AfiI	CCNNNNNNNGG	6 cut(s)	549, 1556, 2060, 2462, 3563, 3745
AflIII	ACRYGT	2 cut(s)	571, 3148
AhdI	GACNNNNNGTC	1 cut(s)	3411
AhlI	ACTAGT	2 cut(s)	2185, 3434
AjnI	CCWGG	4 cut(s)	1287, 2095, 2230, 3849
AjuI	GAANNNNNNNTTGG	2 cut(s)	712, 744
AleI	CACNNNNGTG	1 cut(s)	2204
Alw21I	GWGCWC	1 cut(s)	1244
Alw26I	GTCTC	9 cut(s)	12, 562, 777, 905, 1934, 2207, 3323, 3622, 3943
AlwI	GGATC	4 cut(s)	471, 843, 1325, 2820
AlwNI	CAGNNNCTG	2 cut(s)	160, 2747
Ama87I	CYCGRG	1 cut(s)	3621
Aor13HI	TCCGGA	1 cut(s)	262
AoxI	GGCC	7 cut(s)	36, 806, 1983, 2619, 3208, 3722, 3736
ApoI	RAATTY	7 cut(s)	1747, 2045, 2417, 2918, 3377, 3754, 3880
AseI	ATTAAT	1 cut(s)	1722
Asp700I	GAANNNNTTC	1 cut(s)	1264
AspLEI	GCGC	2 cut(s)	2575, 2834
AspS9I	GGNCC	6 cut(s)	37, 587, 807, 2369, 2452, 3566
AsuC2I	CCSGG	2 cut(s)	2373, 2829
AsuHPI	GGTGA	2 cut(s)	74, 2561
AvaI	CYCGRG	1 cut(s)	3621
AvaII	GGWCC	4 cut(s)	587, 2369, 2452, 3566
AxyI	CCTNAGG	1 cut(s)	3265
BaeGI	GKGCMC	1 cut(s)	116
BalI	TGGCCA	1 cut(s)	1985
BanI	GGYRCC	2 cut(s)	113, 3700
BanII	GRGCYC	2 cut(s)	719, 2990
BauI	CACGAG	2 cut(s)	1269, 3684
BbsI	GAAGAC	3 cut(s)	1398, 1561, 3204
Bbv12I	GWGCWC	1 cut(s)	1244
BccI	CCATC	9 cut(s)	487, 575, 847, 1142, 1145, 1871, 1907, 1994, 2456
BceAI	ACGGC	3 cut(s)	2387, 3284, 3737
BcgI	CGANNNNNNTGC	2 cut(s)	395, 429
BciT130I	CCWGG	4 cut(s)	1289, 2097, 2232, 3851
BciVI	GTATCC	1 cut(s)	2515
BcnI	CCSGG	2 cut(s)	2373, 2829
BcoDI	GTCTC	9 cut(s)	12, 562, 777, 905, 1934, 2207, 3323, 3622, 3943
BcuI	ACTAGT	2 cut(s)	2185, 3434
BfaI	CTAG	5 cut(s)	561, 1335, 2027, 2186, 3435
BfmI	CTRYAG	3 cut(s)	789, 1326, 2949
BfoI	RGCGCY	2 cut(s)	2576, 2835
BfuI	GTATCC	1 cut(s)	2515
BlpI	GCTNAGC	3 cut(s)	762, 1044, 1302
BmcAI	AGTACT	1 cut(s)	1282
Bme1390I	CCNGG	6 cut(s)	1289, 2097, 2232, 2373, 2829, 3851
Bme18I	GGWCC	4 cut(s)	587, 2369, 2452, 3566
BmeRI	GACNNNNNGTC	1 cut(s)	3411
BmeT110I	CYCGRG	1 cut(s)	3621
BmgT120I	GGNCC	6 cut(s)	37, 587, 807, 2369, 2452, 3566
BmiI	GGNNCC	8 cut(s)	115, 716, 939, 2371, 2454, 2987, 3262, 3702
BmrFI	CCNGG	6 cut(s)	1289, 2097, 2232, 2373, 2829, 3851
BmrI	ACTGGG	5 cut(s)	937, 1500, 1587, 2007, 2984
BmuI	ACTGGG	5 cut(s)	937, 1500, 1587, 2007, 2984
BpiI	GAAGAC	3 cut(s)	1398, 1561, 3204
BpmI	CTGGAG	2 cut(s)	2418, 3690
Bpu10I	CCTNAGC	1 cut(s)	3908
Bpu1102I	GCTNAGC	3 cut(s)	762, 1044, 1302
BpuEI	CTTGAG	2 cut(s)	1258, 2638
BpuMI	CCSGG	2 cut(s)	2373, 2829
Bsa29I	ATCGAT	1 cut(s)	491
BsaAI	YACGTR	2 cut(s)	655, 1616
BsaBI	GATNNNNATC	2 cut(s)	1791, 2823
BsaHI	GRCGYC	1 cut(s)	3412
BsaI	GGTCTC	2 cut(s)	777, 2207
BsaJI	CCNNGG	6 cut(s)	767, 1552, 2231, 2544, 2610, 2622
BsaWI	WCCGGW	1 cut(s)	262
BsaXI	ACNNNNNCTCC	2 cut(s)	3026, 3056
Bsc4I	CCNNNNNNNGG	6 cut(s)	549, 1556, 2060, 2462, 3563, 3745
Bse21I	CCTNAGG	1 cut(s)	3265
Bse3DI	GCAATG	5 cut(s)	76, 1950, 2599, 3010, 3106
Bse8I	GATNNNNATC	2 cut(s)	1791, 2823
BseAI	TCCGGA	1 cut(s)	262
BseBI	CCWGG	4 cut(s)	1289, 2097, 2232, 3851
BseCI	ATCGAT	1 cut(s)	491
BseDI	CCNNGG	6 cut(s)	767, 1552, 2231, 2544, 2610, 2622
BseGI	GGATG	5 cut(s)	1819, 1828, 2664, 2824, 3019
BseJI	GATNNNNATC	2 cut(s)	1791, 2823
BseLI	CCNNNNNNNGG	6 cut(s)	549, 1556, 2060, 2462, 3563, 3745
BseMI	GCAATG	5 cut(s)	76, 1950, 2599, 3010, 3106
BseRI	GAGGAG	4 cut(s)	212, 532, 2705, 2998
BseSI	GKGCMC	1 cut(s)	116
BseYI	CCCAGC	2 cut(s)	26, 3928
BsgI	GTGCAG	1 cut(s)	1944
Bsh1236I	CGCG	2 cut(s)	1167, 2154
Bsh1285I	CGRYCG	1 cut(s)	1759
BshFI	GGCC	7 cut(s)	38, 808, 1985, 2621, 3210, 3724, 3738
BshNI	GGYRCC	2 cut(s)	113, 3700
BshVI	ATCGAT	1 cut(s)	491
BsiEI	CGRYCG	1 cut(s)	1759
BsiHKAI	GWGCWC	1 cut(s)	1244
BsiHKCI	CYCGRG	1 cut(s)	3621
BsiSI	CCGG	4 cut(s)	263, 736, 2373, 2829
BslFI	GGGAC	3 cut(s)	1507, 1895, 2438
BslI	CCNNNNNNNGG	6 cut(s)	549, 1556, 2060, 2462, 3563, 3745
BsmAI	GTCTC	9 cut(s)	12, 562, 777, 905, 1934, 2207, 3323, 3622, 3943
BsmBI	CGTCTC	2 cut(s)	12, 1934
BsmFI	GGGAC	3 cut(s)	1507, 1895, 2438
BsmI	GAATGC	1 cut(s)	1266
BsnI	GGCC	7 cut(s)	38, 808, 1985, 2621, 3210, 3724, 3738
Bso31I	GGTCTC	2 cut(s)	777, 2207
BsoBI	CYCGRG	1 cut(s)	3621
Bsp1286I	GDGCHC	4 cut(s)	116, 719, 1244, 2990
Bsp13I	TCCGGA	1 cut(s)	262
Bsp1407I	TGTACA	1 cut(s)	271
Bsp143I	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
Bsp1720I	GCTNAGC	3 cut(s)	762, 1044, 1302
Bsp19I	CCATGG	1 cut(s)	2622
BspACI	CCGC	9 cut(s)	40, 522, 993, 1231, 1430, 2138, 2707, 2744, 3304
BspANI	GGCC	7 cut(s)	38, 808, 1985, 2621, 3210, 3724, 3738
BspDI	ATCGAT	1 cut(s)	491
BspEI	TCCGGA	1 cut(s)	262
BspFNI	CGCG	2 cut(s)	1167, 2154
BspHI	TCATGA	4 cut(s)	1143, 1225, 1638, 2803
BspLI	GGNNCC	8 cut(s)	115, 716, 939, 2371, 2454, 2987, 3262, 3702
BspPI	GGATC	4 cut(s)	471, 843, 1325, 2820
BspQI	GCTCTTC	1 cut(s)	2347
BspT107I	GGYRCC	2 cut(s)	113, 3700
BspTNI	GGTCTC	2 cut(s)	777, 2207
BsrBI	CCGCTC	1 cut(s)	1231
BsrDI	GCAATG	5 cut(s)	76, 1950, 2599, 3010, 3106
BsrGI	TGTACA	1 cut(s)	271
BssECI	CCNNGG	6 cut(s)	767, 1552, 2231, 2544, 2610, 2622
BssMI	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
BssNAI	GTATAC	1 cut(s)	378
BssNI	GRCGYC	1 cut(s)	3412
BssSI	CACGAG	2 cut(s)	1269, 3684
BssT1I	CCWWGG	2 cut(s)	2544, 2622
Bst1107I	GTATAC	1 cut(s)	378
Bst2BI	CACGAG	2 cut(s)	1269, 3684
Bst2UI	CCWGG	4 cut(s)	1289, 2097, 2232, 3851
Bst6I	CTCTTC	2 cut(s)	2347, 3321
BstACI	GRCGYC	1 cut(s)	3412
BstAPI	GCANNNNNTGC	3 cut(s)	1034, 2336, 2747
BstAUI	TGTACA	1 cut(s)	271
BstBAI	YACGTR	2 cut(s)	655, 1616
BstC8I	GCNNGC	8 cut(s)	40, 598, 961, 1169, 2035, 2105, 2136, 3316
BstDSI	CCRYGG	4 cut(s)	767, 1552, 2610, 2622
BstF5I	GGATG	5 cut(s)	1819, 1828, 2664, 2824, 3019
BstFNI	CGCG	2 cut(s)	1167, 2154
BstH2I	RGCGCY	2 cut(s)	2576, 2835
BstHHI	GCGC	2 cut(s)	2575, 2834
BstKTI	GATC	7 cut(s)	455, 466, 838, 1333, 1795, 2182, 2815
BstMAI	GTCTC	9 cut(s)	12, 562, 777, 905, 1934, 2207, 3323, 3622, 3943
BstMBI	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
BstMCI	CGRYCG	1 cut(s)	1759
BstNI	CCWGG	4 cut(s)	1289, 2097, 2232, 3851
BstNSI	RCATGY	5 cut(s)	575, 2111, 3152, 3424, 3683
BstSCI	CCNGG	6 cut(s)	1287, 2095, 2230, 2371, 2827, 3849
BstSFI	CTRYAG	3 cut(s)	789, 1326, 2949
BstSLI	GKGCMC	1 cut(s)	116
BstSNI	TACGTA	2 cut(s)	655, 1616
BstUI	CGCG	2 cut(s)	1167, 2154
BstV2I	GAAGAC	3 cut(s)	1398, 1561, 3204
BstX2I	RGATCY	3 cut(s)	463, 835, 1330
BstXI	CCANNNNNNTGG	1 cut(s)	2206
BstYI	RGATCY	3 cut(s)	463, 835, 1330
BstZ17I	GTATAC	1 cut(s)	378
Bsu15I	ATCGAT	1 cut(s)	491
Bsu36I	CCTNAGG	1 cut(s)	3265
BsuI	GTATCC	1 cut(s)	2515
BsuRI	GGCC	7 cut(s)	38, 808, 1985, 2621, 3210, 3724, 3738
BsuTUI	ATCGAT	1 cut(s)	491
BtgI	CCRYGG	4 cut(s)	767, 1552, 2610, 2622
BtsCI	GGATG	5 cut(s)	1819, 1828, 2664, 2824, 3019
BtsI	GCAGTG	1 cut(s)	2972
BtsIMutI	CAGTG	5 cut(s)	1575, 1958, 2941, 2972, 3390
Cac8I	GCNNGC	8 cut(s)	40, 598, 961, 1169, 2035, 2105, 2136, 3316
CaiI	CAGNNNCTG	2 cut(s)	160, 2747
CciI	TCATGA	4 cut(s)	1143, 1225, 1638, 2803
CfoI	GCGC	2 cut(s)	2575, 2834
Cfr13I	GGNCC	6 cut(s)	37, 587, 807, 2369, 2452, 3566
ClaI	ATCGAT	1 cut(s)	491
Csp6I	GTAC	3 cut(s)	272, 1281, 2686
CspCI	CAANNNNNGTGG	4 cut(s)	1368, 1403, 1891, 1926
CviQI	GTAC	3 cut(s)	272, 1281, 2686
DpnI	GATC	7 cut(s)	454, 465, 837, 1332, 1794, 2181, 2814
DpnII	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
DraI	TTTAAA	1 cut(s)	394
DraIII	CACNNNGTG	1 cut(s)	3301
DriI	GACNNNNNGTC	1 cut(s)	3411
EaeI	YGGCCR	2 cut(s)	1983, 3722
Eam1104I	CTCTTC	2 cut(s)	2347, 3321
Eam1105I	GACNNNNNGTC	1 cut(s)	3411
EarI	CTCTTC	2 cut(s)	2347, 3321
Eco105I	TACGTA	2 cut(s)	655, 1616
Eco130I	CCWWGG	2 cut(s)	2544, 2622
Eco24I	GRGCYC	2 cut(s)	719, 2990
Eco31I	GGTCTC	2 cut(s)	777, 2207
Eco47I	GGWCC	4 cut(s)	587, 2369, 2452, 3566
Eco57I	CTGAAG	3 cut(s)	1157, 1608, 3297
Eco81I	CCTNAGG	1 cut(s)	3265
Eco88I	CYCGRG	1 cut(s)	3621
EcoO109I	RGGNCCY	2 cut(s)	2369, 2452
EcoRII	CCWGG	4 cut(s)	1287, 2095, 2230, 3849
EcoT14I	CCWWGG	2 cut(s)	2544, 2622
EcoT22I	ATGCAT	3 cut(s)	76, 3122, 3426
EcoT38I	GRGCYC	2 cut(s)	719, 2990
ErhI	CCWWGG	2 cut(s)	2544, 2622
Esp3I	CGTCTC	2 cut(s)	12, 1934
FalI	AAGNNNNNCTT	2 cut(s)	964, 996
FaqI	GGGAC	3 cut(s)	1507, 1895, 2438
FauI	CCCGC	4 cut(s)	47, 986, 1437, 2714
FauNDI	CATATG	2 cut(s)	1057, 2728
FblI	GTMKAC	2 cut(s)	377, 1069
FokI	GGATG	5 cut(s)	1826, 1835, 2671, 2831, 3006
FriOI	GRGCYC	2 cut(s)	719, 2990
FspBI	CTAG	5 cut(s)	561, 1335, 2027, 2186, 3435
GlaI	GCGC	2 cut(s)	2574, 2833
GsaI	CCCAGC	2 cut(s)	30, 3932
GsuI	CTGGAG	2 cut(s)	2418, 3690
HaeII	RGCGCY	2 cut(s)	2576, 2835
HaeIII	GGCC	7 cut(s)	38, 808, 1985, 2621, 3210, 3724, 3738
HapII	CCGG	4 cut(s)	263, 736, 2373, 2829
HhaI	GCGC	2 cut(s)	2575, 2834
Hin1I	GRCGYC	1 cut(s)	3412
Hin6I	GCGC	2 cut(s)	2573, 2832
HinP1I	GCGC	2 cut(s)	2573, 2832
HincII	GTYRAC	1 cut(s)	1768
HindII	GTYRAC	1 cut(s)	1768
HindIII	AAGCTT	3 cut(s)	257, 1358, 3581
HinfI	GANTC	8 cut(s)	314, 437, 692, 1595, 1835, 2641, 2792, 3823
HpaII	CCGG	4 cut(s)	263, 736, 2373, 2829
HphI	GGTGA	2 cut(s)	74, 2561
Hpy166II	GTNNAC	9 cut(s)	272, 378, 431, 1070, 1768, 2842, 3295, 3388, 3599
Hpy8I	GTNNAC	9 cut(s)	272, 378, 431, 1070, 1768, 2842, 3295, 3388, 3599
Hpy99I	CGWCG	1 cut(s)	1023
HpyCH4IV	ACGT	6 cut(s)	331, 654, 1615, 2344, 2560, 3412
HpySE526I	ACGT	6 cut(s)	331, 654, 1615, 2344, 2560, 3412
Hsp92I	GRCGYC	1 cut(s)	3412
HspAI	GCGC	2 cut(s)	2573, 2832
Kpn2I	TCCGGA	1 cut(s)	262
Kzo9I	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
LguI	GCTCTTC	1 cut(s)	2347
MaeI	CTAG	5 cut(s)	561, 1335, 2027, 2186, 3435
MaeII	ACGT	6 cut(s)	331, 654, 1615, 2344, 2560, 3412
MalI	GATC	7 cut(s)	454, 465, 837, 1332, 1794, 2181, 2814
MbiI	CCGCTC	1 cut(s)	1231
MboI	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
MflI	RGATCY	3 cut(s)	463, 835, 1330
MhlI	GDGCHC	4 cut(s)	116, 719, 1244, 2990
MlsI	TGGCCA	1 cut(s)	1985
MluNI	TGGCCA	1 cut(s)	1985
MlyI	GAGTC	5 cut(s)	446, 701, 1604, 2650, 2786
MmeI	TCCRAC	4 cut(s)	174, 809, 2546, 3091
Mox20I	TGGCCA	1 cut(s)	1985
Mph1103I	ATGCAT	3 cut(s)	76, 3122, 3426
MroI	TCCGGA	1 cut(s)	262
MroXI	GAANNNNTTC	1 cut(s)	1264
MscI	TGGCCA	1 cut(s)	1985
MslI	CAYNNNNRTG	3 cut(s)	529, 2204, 3684
Msp20I	TGGCCA	1 cut(s)	1985
MspA1I	CMGCKG	5 cut(s)	1309, 2336, 2438, 2744, 3356
MspI	CCGG	4 cut(s)	263, 736, 2373, 2829
MspR9I	CCNGG	6 cut(s)	1289, 2097, 2232, 2373, 2829, 3851
Mva1269I	GAATGC	1 cut(s)	1266
MvaI	CCWGG	4 cut(s)	1289, 2097, 2232, 3851
MvnI	CGCG	2 cut(s)	1167, 2154
NciI	CCSGG	2 cut(s)	2373, 2829
NcoI	CCATGG	1 cut(s)	2622
NdeI	CATATG	2 cut(s)	1057, 2728
NdeII	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
NlaIV	GGNNCC	8 cut(s)	115, 716, 939, 2371, 2454, 2987, 3262, 3702
NmeAIII	GCCGAG	1 cut(s)	3467
NmuCI	GTSAC	5 cut(s)	574, 1006, 1573, 3526, 3625
NsiI	ATGCAT	3 cut(s)	76, 3122, 3426
NspI	RCATGY	5 cut(s)	575, 2111, 3152, 3424, 3683
OliI	CACNNNNGTG	1 cut(s)	2204
PaeR7I	CTCGAG	1 cut(s)	3621
PagI	TCATGA	4 cut(s)	1143, 1225, 1638, 2803
PciI	ACATGT	2 cut(s)	571, 3148
PciSI	GCTCTTC	1 cut(s)	2347
PcsI	WCGNNNNNNNCGW	1 cut(s)	1612
PctI	GAATGC	1 cut(s)	1266
PdmI	GAANNNNTTC	1 cut(s)	1264
PfeI	GAWTC	3 cut(s)	314, 1835, 3823
PflMI	CCANNNNNTGG	1 cut(s)	3563
PfoI	TCCNGGA	1 cut(s)	3849
PleI	GAGTC	5 cut(s)	445, 700, 1603, 2649, 2786
PpsI	GAGTC	5 cut(s)	445, 700, 1603, 2649, 2786
Ppu21I	YACGTR	2 cut(s)	655, 1616
PpuMI	RGGWCCY	2 cut(s)	2369, 2452
PscI	ACATGT	2 cut(s)	571, 3148
PshBI	ATTAAT	1 cut(s)	1722
Psp1406I	AACGTT	1 cut(s)	2560
Psp5II	RGGWCCY	2 cut(s)	2369, 2452
Psp6I	CCWGG	4 cut(s)	1287, 2095, 2230, 3849
PspFI	CCCAGC	2 cut(s)	26, 3928
PspGI	CCWGG	4 cut(s)	1287, 2095, 2230, 3849
PspN4I	GGNNCC	8 cut(s)	115, 716, 939, 2371, 2454, 2987, 3262, 3702
PspPI	GGNCC	6 cut(s)	37, 587, 807, 2369, 2452, 3566
PspPPI	RGGWCCY	2 cut(s)	2369, 2452
PstNI	CAGNNNCTG	2 cut(s)	160, 2747
PsuI	RGATCY	3 cut(s)	463, 835, 1330
PvuII	CAGCTG	4 cut(s)	1309, 2336, 2438, 3356
RsaI	GTAC	3 cut(s)	273, 1282, 2687
RsaNI	GTAC	3 cut(s)	272, 1281, 2686
RseI	CAYNNNNRTG	3 cut(s)	529, 2204, 3684
SapI	GCTCTTC	1 cut(s)	2347
Sau3AI	GATC	7 cut(s)	452, 463, 835, 1330, 1792, 2179, 2812
Sau96I	GGNCC	6 cut(s)	37, 587, 807, 2369, 2452, 3566
ScaI	AGTACT	1 cut(s)	1282
SchI	GAGTC	5 cut(s)	446, 701, 1604, 2650, 2786
ScrFI	CCNGG	6 cut(s)	1289, 2097, 2232, 2373, 2829, 3851
SduI	GDGCHC	4 cut(s)	116, 719, 1244, 2990
SfcI	CTRYAG	3 cut(s)	789, 1326, 2949
Sfr274I	CTCGAG	1 cut(s)	3621
SinI	GGWCC	4 cut(s)	587, 2369, 2452, 3566
SlaI	CTCGAG	1 cut(s)	3621
SmiMI	CAYNNNNRTG	3 cut(s)	529, 2204, 3684
SmlI	CTYRAG	3 cut(s)	1237, 2653, 3621
SmoI	CTYRAG	3 cut(s)	1237, 2653, 3621
SnaBI	TACGTA	2 cut(s)	655, 1616
SpeI	ACTAGT	2 cut(s)	2185, 3434
SsiI	CCGC	9 cut(s)	40, 522, 993, 1231, 1430, 2138, 2707, 2744, 3304
SspI	AATATT	3 cut(s)	1158, 2445, 2494
SspMI	CTAG	5 cut(s)	561, 1335, 2027, 2186, 3435
StyD4I	CCNGG	6 cut(s)	1287, 2095, 2230, 2371, 2827, 3849
StyI	CCWWGG	2 cut(s)	2544, 2622
TaiI	ACGT	6 cut(s)	334, 657, 1618, 2347, 2563, 3415
TaqI	TCGA	9 cut(s)	405, 411, 451, 491, 988, 1606, 2182, 2981, 3622
TaqII	GACCGA	1 cut(s)	3549
TatI	WGTACW	2 cut(s)	271, 1280
TauI	GCSGC	2 cut(s)	2141, 2747
TfiI	GAWTC	3 cut(s)	314, 1835, 3823
TscAI	CASTG	5 cut(s)	1582, 1965, 2941, 2972, 3397
TseFI	GTSAC	5 cut(s)	574, 1006, 1573, 3526, 3625
Tsp45I	GTSAC	5 cut(s)	574, 1006, 1573, 3526, 3625
TspGWI	ACGGA	7 cut(s)	36, 363, 1259, 1341, 1511, 2039, 3459
TspRI	CASTG	5 cut(s)	1582, 1965, 2941, 2972, 3397
Van91I	CCANNNNNTGG	1 cut(s)	3563
VpaK11BI	GGWCC	4 cut(s)	587, 2369, 2452, 3566
VspI	ATTAAT	1 cut(s)	1722
XapI	RAATTY	7 cut(s)	1747, 2045, 2417, 2918, 3377, 3754, 3880
XceI	RCATGY	5 cut(s)	575, 2111, 3152, 3424, 3683
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1384, 3746
XhoI	CTCGAG	1 cut(s)	3621
XmiI	GTMKAC	2 cut(s)	377, 1069
XmnI	GAANNNNTTC	1 cut(s)	1264
XspI	CTAG	5 cut(s)	561, 1335, 2027, 2186, 3435
ZraI	GACGTC	1 cut(s)	3413
ZrmI	AGTACT	1 cut(s)	1282
Zsp2I	ATGCAT	3 cut(s)	76, 3122, 3426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.