ATP-dependent RNA helicase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Helicase_C | PF00271 | 637 - 723 | 1.8e-09 | Helicase conserved C-terminal domain |
| WHD_HA2 | PF04408 | 758 - 786 | 4.4e-07 | Helicase associated domain (HA2), winged-helix |
| OB_NTP_bind | PF07717 | 968 - 1042 | 1.6e-17 | Oligonucleotide/oligosaccharide-binding (OB)-fold |
| DHX37_C | PF23362 | 1096 - 1157 | 1.3e-11 | DEAH helicase DHX37 C-terminal domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1496 |
| Acc36I | ACCTGC | 1 cut(s) | 3302 |
| AccB1I | GGYRCC | 1 cut(s) | 906 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 3230 |
| AccBSI | CCGCTC | 1 cut(s) | 1126 |
| AccII | CGCG | 3 cut(s) | 12, 222, 1909 |
| AccIII | TCCGGA | 2 cut(s) | 3418, 3426 |
| AciI | CCGC | 8 cut(s) | 222, 718, 903, 946, 1126, 1907, 2201, 3176 |
| AclWI | GGATC | 7 cut(s) | 381, 396, 1312, 2286, 2577, 2831, 3052 |
| AcoI | YGGCCR | 3 cut(s) | 231, 1797, 2189 |
| AcsI | RAATTY | 6 cut(s) | 1034, 1392, 2228, 2620, 2813, 3468 |
| AcuI | CTGAAG | 7 cut(s) | 143, 196, 1371, 1545, 1796, 1797, 1809 |
| AdeI | CACNNNGTG | 1 cut(s) | 1910 |
| AfaI | GTAC | 6 cut(s) | 79, 289, 1852, 2115, 3047, 3083 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1415, 2252, 3230 |
| AflIII | ACRYGT | 1 cut(s) | 546 |
| AgeI | ACCGGT | 1 cut(s) | 2218 |
| AjnI | CCWGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2779, 2811, 3265, 3297 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 264, 296 |
| Alw21I | GWGCWC | 7 cut(s) | 201, 581, 1139, 1272, 2548, 2901, 3158 |
| Alw26I | GTCTC | 5 cut(s) | 514, 555, 1136, 1685, 2981 |
| Alw44I | GTGCAC | 4 cut(s) | 197, 577, 1135, 3154 |
| AlwI | GGATC | 7 cut(s) | 381, 396, 1312, 2286, 2577, 2831, 3052 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 962, 2187 |
| Ama87I | CYCGRG | 1 cut(s) | 2424 |
| Aor13HI | TCCGGA | 2 cut(s) | 3418, 3426 |
| AoxI | GGCC | 9 cut(s) | 167, 231, 1779, 1797, 2184, 2189, 2337, 2970, 3299 |
| ApaLI | GTGCAC | 4 cut(s) | 197, 577, 1135, 3154 |
| ApoI | RAATTY | 6 cut(s) | 1034, 1392, 2228, 2620, 2813, 3468 |
| AseI | ATTAAT | 1 cut(s) | 1365 |
| AsiGI | ACCGGT | 1 cut(s) | 2218 |
| Asp700I | GAANNNNTTC | 7 cut(s) | 357, 1159, 1807, 1986, 2622, 3074, 3324 |
| AspS9I | GGNCC | 2 cut(s) | 1117, 2184 |
| AsuHPI | GGTGA | 7 cut(s) | 1028, 1085, 1730, 1896, 2267, 2332, 3003 |
| AsuNHI | GCTAGC | 1 cut(s) | 2440 |
| AvaI | CYCGRG | 1 cut(s) | 2424 |
| AvaII | GGWCC | 1 cut(s) | 1117 |
| BaeGI | GKGCMC | 5 cut(s) | 201, 581, 911, 1139, 3158 |
| BalI | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| BanI | GGYRCC | 1 cut(s) | 906 |
| BanII | GRGCYC | 1 cut(s) | 2901 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2886, 2918 |
| BauI | CACGAG | 1 cut(s) | 735 |
| BbsI | GAAGAC | 3 cut(s) | 484, 1302, 2604 |
| Bbv12I | GWGCWC | 7 cut(s) | 201, 581, 1139, 1272, 2548, 2901, 3158 |
| BccI | CCATC | 4 cut(s) | 772, 1040, 1613, 1617 |
| BceAI | ACGGC | 3 cut(s) | 334, 3100, 3354 |
| BciT130I | CCWGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| BciVI | GTATCC | 2 cut(s) | 2233, 3426 |
| BcoDI | GTCTC | 5 cut(s) | 514, 555, 1136, 1685, 2981 |
| BfaI | CTAG | 3 cut(s) | 464, 1706, 2441 |
| BfmI | CTRYAG | 3 cut(s) | 554, 1938, 2454 |
| BfuAI | ACCTGC | 1 cut(s) | 3302 |
| BfuI | GTATCC | 2 cut(s) | 2233, 3426 |
| BlpI | GCTNAGC | 2 cut(s) | 972, 1947 |
| Bme1390I | CCNGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| Bme18I | GGWCC | 1 cut(s) | 1117 |
| BmeT110I | CYCGRG | 1 cut(s) | 2424 |
| BmgT120I | GGNCC | 2 cut(s) | 1117, 2184 |
| BmiI | GGNNCC | 1 cut(s) | 908 |
| BmrFI | CCNGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| BmrI | ACTGGG | 1 cut(s) | 1446 |
| BmsI | GCATC | 9 cut(s) | 739, 900, 1392, 1675, 1778, 2141, 2367, 2632, 3471 |
| BmtI | GCTAGC | 1 cut(s) | 2444 |
| BmuI | ACTGGG | 1 cut(s) | 1446 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2523 |
| BpiI | GAAGAC | 3 cut(s) | 484, 1302, 2604 |
| BpmI | CTGGAG | 2 cut(s) | 2532, 2820 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 972, 1947 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1641 |
| BsaJI | CCNNGG | 7 cut(s) | 170, 234, 1069, 1888, 2033, 2340, 2733 |
| BsaWI | WCCGGW | 3 cut(s) | 2218, 3418, 3426 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1415, 2252, 3230 |
| Bse118I | RCCGGY | 1 cut(s) | 2218 |
| Bse1I | ACTGG | 5 cut(s) | 1289, 1441, 2062, 2549, 3008 |
| Bse3DI | GCAATG | 4 cut(s) | 104, 951, 1764, 2317 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1641 |
| BseAI | TCCGGA | 2 cut(s) | 3418, 3426 |
| BseBI | CCWGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| BseDI | CCNNGG | 7 cut(s) | 170, 234, 1069, 1888, 2033, 2340, 2733 |
| BseGI | GGATG | 4 cut(s) | 31, 503, 848, 2869 |
| BseJI | GATNNNNATC | 1 cut(s) | 1641 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1415, 2252, 3230 |
| BseMI | GCAATG | 4 cut(s) | 104, 951, 1764, 2317 |
| BseNI | ACTGG | 5 cut(s) | 1289, 1441, 2062, 2549, 3008 |
| BseRI | GAGGAG | 3 cut(s) | 145, 2235, 2756 |
| BseSI | GKGCMC | 5 cut(s) | 201, 581, 911, 1139, 3158 |
| BseYI | CCCAGC | 2 cut(s) | 657, 3346 |
| BsgI | GTGCAG | 2 cut(s) | 522, 669 |
| Bsh1236I | CGCG | 3 cut(s) | 12, 222, 1909 |
| BshFI | GGCC | 9 cut(s) | 169, 233, 1781, 1799, 2186, 2191, 2339, 2972, 3301 |
| BshNI | GGYRCC | 1 cut(s) | 906 |
| BshTI | ACCGGT | 1 cut(s) | 2218 |
| BsiHKAI | GWGCWC | 7 cut(s) | 201, 581, 1139, 1272, 2548, 2901, 3158 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2424 |
| BsiSI | CCGG | 3 cut(s) | 2219, 3419, 3427 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1415, 2252, 3230 |
| BsmAI | GTCTC | 5 cut(s) | 514, 555, 1136, 1685, 2981 |
| BsmBI | CGTCTC | 1 cut(s) | 1136 |
| BsmI | GAATGC | 2 cut(s) | 811, 1161 |
| BsnI | GGCC | 9 cut(s) | 169, 233, 1781, 1799, 2186, 2191, 2339, 2972, 3301 |
| BsoBI | CYCGRG | 1 cut(s) | 2424 |
| Bsp1286I | GDGCHC | 8 cut(s) | 201, 581, 911, 1139, 1272, 2548, 2901, 3158 |
| Bsp13I | TCCGGA | 2 cut(s) | 3418, 3426 |
| Bsp1407I | TGTACA | 2 cut(s) | 77, 3045 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 972, 1947 |
| Bsp19I | CCATGG | 2 cut(s) | 2340, 2733 |
| Bsp68I | TCGCGA | 1 cut(s) | 12 |
| BspACI | CCGC | 8 cut(s) | 222, 718, 903, 946, 1126, 1907, 2201, 3176 |
| BspANI | GGCC | 9 cut(s) | 169, 233, 1781, 1799, 2186, 2191, 2339, 2972, 3301 |
| BspEI | TCCGGA | 2 cut(s) | 3418, 3426 |
| BspFNI | CGCG | 3 cut(s) | 12, 222, 1909 |
| BspHI | TCATGA | 3 cut(s) | 1038, 1272, 1882 |
| BspLI | GGNNCC | 1 cut(s) | 908 |
| BspMAI | CTGCAG | 2 cut(s) | 1942, 2458 |
| BspMI | ACCTGC | 1 cut(s) | 3302 |
| BspOI | GCTAGC | 1 cut(s) | 2444 |
| BspPI | GGATC | 7 cut(s) | 381, 396, 1312, 2286, 2577, 2831, 3052 |
| BspQI | GCTCTTC | 4 cut(s) | 212, 2149, 2162, 2889 |
| BspT107I | GGYRCC | 1 cut(s) | 906 |
| BsrBI | CCGCTC | 1 cut(s) | 1126 |
| BsrDI | GCAATG | 4 cut(s) | 104, 951, 1764, 2317 |
| BsrFI | RCCGGY | 1 cut(s) | 2218 |
| BsrGI | TGTACA | 2 cut(s) | 77, 3045 |
| BsrI | ACTGG | 5 cut(s) | 1289, 1441, 2062, 2549, 3008 |
| BssAI | RCCGGY | 1 cut(s) | 2218 |
| BssECI | CCNNGG | 7 cut(s) | 170, 234, 1069, 1888, 2033, 2340, 2733 |
| BssSI | CACGAG | 1 cut(s) | 735 |
| BssT1I | CCWWGG | 3 cut(s) | 170, 2340, 2733 |
| Bst2BI | CACGAG | 1 cut(s) | 735 |
| Bst2UI | CCWGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| Bst6I | CTCTTC | 7 cut(s) | 122, 212, 1082, 1653, 2149, 2162, 2889 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 327, 962 |
| BstAUI | TGTACA | 2 cut(s) | 77, 3045 |
| BstC8I | GCNNGC | 8 cut(s) | 119, 328, 1831, 1938, 2205, 2442, 2974, 3132 |
| BstDSI | CCRYGG | 2 cut(s) | 2340, 2733 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| BstF5I | GGATG | 4 cut(s) | 31, 503, 848, 2869 |
| BstFNI | CGCG | 3 cut(s) | 12, 222, 1909 |
| BstMAI | GTCTC | 5 cut(s) | 514, 555, 1136, 1685, 2981 |
| BstNI | CCWGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| BstNSI | RCATGY | 2 cut(s) | 205, 550 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2523 |
| BstSCI | CCNGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| BstSFI | CTRYAG | 3 cut(s) | 554, 1938, 2454 |
| BstSLI | GKGCMC | 5 cut(s) | 201, 581, 911, 1139, 3158 |
| BstUI | CGCG | 3 cut(s) | 12, 222, 1909 |
| BstV2I | GAAGAC | 3 cut(s) | 484, 1302, 2604 |
| BstX2I | RGATCY | 3 cut(s) | 373, 1317, 2823 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1291 |
| BstYI | RGATCY | 3 cut(s) | 373, 1317, 2823 |
| BsuI | GTATCC | 2 cut(s) | 2233, 3426 |
| BsuRI | GGCC | 9 cut(s) | 169, 233, 1781, 1799, 2186, 2191, 2339, 2972, 3301 |
| BtgI | CCRYGG | 2 cut(s) | 2340, 2733 |
| BtgZI | GCGATG | 1 cut(s) | 203 |
| BtsCI | GGATG | 4 cut(s) | 31, 503, 848, 2869 |
| BtsI | GCAGTG | 4 cut(s) | 328, 448, 960, 2644 |
| BtuMI | TCGCGA | 1 cut(s) | 12 |
| BveI | ACCTGC | 1 cut(s) | 3302 |
| Cac8I | GCNNGC | 8 cut(s) | 119, 328, 1831, 1938, 2205, 2442, 2974, 3132 |
| CaiI | CAGNNNCTG | 2 cut(s) | 962, 2187 |
| CciI | TCATGA | 3 cut(s) | 1038, 1272, 1882 |
| Cfr10I | RCCGGY | 1 cut(s) | 2218 |
| Cfr13I | GGNCC | 2 cut(s) | 1117, 2184 |
| CseI | GACGC | 3 cut(s) | 1153, 1205, 1940 |
| CsiI | ACCWGGT | 1 cut(s) | 1897 |
| Csp6I | GTAC | 6 cut(s) | 78, 288, 1851, 2114, 3046, 3082 |
| CspAI | ACCGGT | 1 cut(s) | 2218 |
| CviQI | GTAC | 6 cut(s) | 78, 288, 1851, 2114, 3046, 3082 |
| DraIII | CACNNNGTG | 1 cut(s) | 1910 |
| EaeI | YGGCCR | 3 cut(s) | 231, 1797, 2189 |
| Eam1104I | CTCTTC | 7 cut(s) | 122, 212, 1082, 1653, 2149, 2162, 2889 |
| EarI | CTCTTC | 7 cut(s) | 122, 212, 1082, 1653, 2149, 2162, 2889 |
| EciI | GGCGGA | 1 cut(s) | 3165 |
| Ecl136II | GAGCTC | 1 cut(s) | 2899 |
| Eco130I | CCWWGG | 3 cut(s) | 170, 2340, 2733 |
| Eco147I | AGGCCT | 3 cut(s) | 169, 1781, 2972 |
| Eco24I | GRGCYC | 1 cut(s) | 2901 |
| Eco47I | GGWCC | 1 cut(s) | 1117 |
| Eco53kI | GAGCTC | 1 cut(s) | 2899 |
| Eco57I | CTGAAG | 7 cut(s) | 143, 196, 1371, 1545, 1796, 1797, 1809 |
| Eco88I | CYCGRG | 1 cut(s) | 2424 |
| EcoICRI | GAGCTC | 1 cut(s) | 2899 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2184 |
| EcoRII | CCWGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| EcoT14I | CCWWGG | 3 cut(s) | 170, 2340, 2733 |
| EcoT22I | ATGCAT | 1 cut(s) | 3141 |
| EcoT38I | GRGCYC | 1 cut(s) | 2901 |
| ErhI | CCWWGG | 3 cut(s) | 170, 2340, 2733 |
| Esp3I | CGTCTC | 1 cut(s) | 1136 |
| FauI | CCCGC | 2 cut(s) | 896, 953 |
| FauNDI | CATATG | 1 cut(s) | 2446 |
| FokI | GGATG | 4 cut(s) | 38, 510, 855, 2876 |
| FriOI | GRGCYC | 1 cut(s) | 2901 |
| FspBI | CTAG | 3 cut(s) | 464, 1706, 2441 |
| GsaI | CCCAGC | 2 cut(s) | 661, 3350 |
| GsuI | CTGGAG | 2 cut(s) | 2532, 2820 |
| HaeIII | GGCC | 9 cut(s) | 169, 233, 1781, 1799, 2186, 2191, 2339, 2972, 3301 |
| HapII | CCGG | 3 cut(s) | 2219, 3419, 3427 |
| HgaI | GACGC | 3 cut(s) | 1153, 1205, 1940 |
| HincII | GTYRAC | 2 cut(s) | 2053, 2554 |
| HindII | GTYRAC | 2 cut(s) | 2053, 2554 |
| HindIII | AAGCTT | 4 cut(s) | 1262, 1791, 2288, 2873 |
| HinfI | GANTC | 8 cut(s) | 186, 341, 637, 704, 1673, 1730, 1817, 1859 |
| HpaI | GTTAAC | 1 cut(s) | 2554 |
| HpaII | CCGG | 3 cut(s) | 2219, 3419, 3427 |
| HphI | GGTGA | 7 cut(s) | 1028, 1085, 1730, 1896, 2267, 2332, 3003 |
| Hpy166II | GTNNAC | 8 cut(s) | 199, 579, 1137, 1904, 2053, 2554, 3046, 3156 |
| Hpy8I | GTNNAC | 8 cut(s) | 199, 579, 1137, 1904, 2053, 2554, 3046, 3156 |
| HpyCH4IV | ACGT | 1 cut(s) | 2146 |
| HpySE526I | ACGT | 1 cut(s) | 2146 |
| Kpn2I | TCCGGA | 2 cut(s) | 3418, 3426 |
| KspAI | GTTAAC | 1 cut(s) | 2554 |
| LguI | GCTCTTC | 4 cut(s) | 212, 2149, 2162, 2889 |
| LmnI | GCTCC | 7 cut(s) | 781, 1128, 2551, 2769, 3034, 3250, 3429 |
| LweI | GCATC | 9 cut(s) | 739, 900, 1392, 1675, 1778, 2141, 2367, 2632, 3471 |
| MabI | ACCWGGT | 1 cut(s) | 1897 |
| MaeI | CTAG | 3 cut(s) | 464, 1706, 2441 |
| MaeII | ACGT | 1 cut(s) | 2146 |
| MaeIII | GTNAC | 9 cut(s) | 533, 934, 958, 1001, 2320, 2348, 2501, 3009, 3217 |
| MbiI | CCGCTC | 1 cut(s) | 1126 |
| MfeI | CAATTG | 1 cut(s) | 1506 |
| MflI | RGATCY | 3 cut(s) | 373, 1317, 2823 |
| MhlI | GDGCHC | 8 cut(s) | 201, 581, 911, 1139, 1272, 2548, 2901, 3158 |
| MlsI | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| MluNI | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| MlyI | GAGTC | 3 cut(s) | 646, 1811, 1853 |
| MmeI | TCCRAC | 4 cut(s) | 330, 964, 1650, 2906 |
| Mox20I | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| Mph1103I | ATGCAT | 1 cut(s) | 3141 |
| MroI | TCCGGA | 2 cut(s) | 3418, 3426 |
| MroXI | GAANNNNTTC | 7 cut(s) | 357, 1159, 1807, 1986, 2622, 3074, 3324 |
| MscI | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| MseI | TTAA | 8 cut(s) | 255, 1365, 2024, 2553, 2781, 3276, 3296, 3438 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1154, 1289 |
| Msp20I | TGGCCA | 3 cut(s) | 233, 1799, 2191 |
| MspA1I | CMGCKG | 3 cut(s) | 506, 948, 2453 |
| MspI | CCGG | 3 cut(s) | 2219, 3419, 3427 |
| MspR9I | CCNGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| MunI | CAATTG | 1 cut(s) | 1506 |
| Mva1269I | GAATGC | 2 cut(s) | 811, 1161 |
| MvaI | CCWGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| MvnI | CGCG | 3 cut(s) | 12, 222, 1909 |
| NcoI | CCATGG | 2 cut(s) | 2340, 2733 |
| NdeI | CATATG | 1 cut(s) | 2446 |
| NheI | GCTAGC | 1 cut(s) | 2440 |
| NlaIV | GGNNCC | 1 cut(s) | 908 |
| NmuCI | GTSAC | 6 cut(s) | 958, 1001, 2320, 2501, 3009, 3217 |
| NruI | TCGCGA | 1 cut(s) | 12 |
| NsiI | ATGCAT | 1 cut(s) | 3141 |
| NspI | RCATGY | 2 cut(s) | 205, 550 |
| PagI | TCATGA | 3 cut(s) | 1038, 1272, 1882 |
| PceI | AGGCCT | 3 cut(s) | 169, 1781, 2972 |
| PciI | ACATGT | 1 cut(s) | 546 |
| PciSI | GCTCTTC | 4 cut(s) | 212, 2149, 2162, 2889 |
| PctI | GAATGC | 2 cut(s) | 811, 1161 |
| PdmI | GAANNNNTTC | 7 cut(s) | 357, 1159, 1807, 1986, 2622, 3074, 3324 |
| PfeI | GAWTC | 5 cut(s) | 186, 341, 704, 1673, 1730 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 3230 |
| PinAI | ACCGGT | 1 cut(s) | 2218 |
| PleI | GAGTC | 3 cut(s) | 645, 1811, 1853 |
| PpsI | GAGTC | 3 cut(s) | 645, 1811, 1853 |
| PscI | ACATGT | 1 cut(s) | 546 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2523 |
| PshBI | ATTAAT | 1 cut(s) | 1365 |
| PsiI | TTATAA | 1 cut(s) | 1496 |
| Psp124BI | GAGCTC | 1 cut(s) | 2901 |
| Psp6I | CCWGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| PspFI | CCCAGC | 2 cut(s) | 657, 3346 |
| PspGI | CCWGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| PspN4I | GGNNCC | 1 cut(s) | 908 |
| PspPI | GGNCC | 2 cut(s) | 1117, 2184 |
| PstI | CTGCAG | 2 cut(s) | 1942, 2458 |
| PstNI | CAGNNNCTG | 2 cut(s) | 962, 2187 |
| PsuI | RGATCY | 3 cut(s) | 373, 1317, 2823 |
| PvuII | CAGCTG | 2 cut(s) | 506, 2453 |
| RruI | TCGCGA | 1 cut(s) | 12 |
| RsaI | GTAC | 6 cut(s) | 79, 289, 1852, 2115, 3047, 3083 |
| RsaNI | GTAC | 6 cut(s) | 78, 288, 1851, 2114, 3046, 3082 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1154, 1289 |
| SacI | GAGCTC | 1 cut(s) | 2901 |
| SapI | GCTCTTC | 4 cut(s) | 212, 2149, 2162, 2889 |
| SaqAI | TTAA | 8 cut(s) | 255, 1365, 2024, 2553, 2781, 3276, 3296, 3438 |
| Sau96I | GGNCC | 2 cut(s) | 1117, 2184 |
| SchI | GAGTC | 3 cut(s) | 646, 1811, 1853 |
| ScrFI | CCNGG | 6 cut(s) | 235, 1070, 1890, 1899, 2034, 2188 |
| SduI | GDGCHC | 8 cut(s) | 201, 581, 911, 1139, 1272, 2548, 2901, 3158 |
| SexAI | ACCWGGT | 1 cut(s) | 1897 |
| SfaNI | GCATC | 9 cut(s) | 739, 900, 1392, 1675, 1778, 2141, 2367, 2632, 3471 |
| SfcI | CTRYAG | 3 cut(s) | 554, 1938, 2454 |
| SinI | GGWCC | 1 cut(s) | 1117 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1154, 1289 |
| SseBI | AGGCCT | 3 cut(s) | 169, 1781, 2972 |
| SsiI | CCGC | 8 cut(s) | 222, 718, 903, 946, 1126, 1907, 2201, 3176 |
| SspI | AATATT | 3 cut(s) | 228, 571, 1053 |
| SspMI | CTAG | 3 cut(s) | 464, 1706, 2441 |
| SstI | GAGCTC | 1 cut(s) | 2901 |
| StuI | AGGCCT | 3 cut(s) | 169, 1781, 2972 |
| StyD4I | CCNGG | 6 cut(s) | 233, 1068, 1888, 1897, 2032, 2186 |
| StyI | CCWWGG | 3 cut(s) | 170, 2340, 2733 |
| TaiI | ACGT | 1 cut(s) | 2149 |
| TaqI | TCGA | 2 cut(s) | 154, 423 |
| TaqII | GACCGA | 1 cut(s) | 3463 |
| TatI | WGTACW | 4 cut(s) | 77, 1850, 2113, 3045 |
| TauI | GCSGC | 1 cut(s) | 721 |
| TfiI | GAWTC | 5 cut(s) | 186, 341, 704, 1673, 1730 |
| Tru1I | TTAA | 8 cut(s) | 255, 1365, 2024, 2553, 2781, 3276, 3296, 3438 |
| Tru9I | TTAA | 8 cut(s) | 255, 1365, 2024, 2553, 2781, 3276, 3296, 3438 |
| TseFI | GTSAC | 6 cut(s) | 958, 1001, 2320, 2501, 3009, 3217 |
| Tsp45I | GTSAC | 6 cut(s) | 958, 1001, 2320, 2501, 3009, 3217 |
| TspGWI | ACGGA | 1 cut(s) | 323 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 3230 |
| VneI | GTGCAC | 4 cut(s) | 197, 577, 1135, 3154 |
| VpaK11BI | GGWCC | 1 cut(s) | 1117 |
| VspI | ATTAAT | 1 cut(s) | 1365 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| XapI | RAATTY | 6 cut(s) | 1034, 1392, 2228, 2620, 2813, 3468 |
| XceI | RCATGY | 2 cut(s) | 205, 550 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 654, 1616, 2692 |
| XmnI | GAANNNNTTC | 7 cut(s) | 357, 1159, 1807, 1986, 2622, 3074, 3324 |
| XspI | CTAG | 3 cut(s) | 464, 1706, 2441 |
| Zsp2I | ATGCAT | 1 cut(s) | 3141 |