ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 3409 |
| AccB1I | GGYRCC | 1 cut(s) | 3694 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2803, 3557 |
| AccBSI | CCGCTC | 1 cut(s) | 1204 |
| AccII | CGCG | 2 cut(s) | 1140, 2148 |
| AccIII | TCCGGA | 1 cut(s) | 244 |
| AclI | AACGTT | 1 cut(s) | 2554 |
| AclWI | GGATC | 4 cut(s) | 453, 816, 1307, 2814 |
| AcoI | YGGCCR | 4 cut(s) | 286, 1489, 1962, 3716 |
| AcsI | RAATTY | 8 cut(s) | 7, 719, 1088, 1729, 2411, 2912, 3748, 3874 |
| AcuI | CTGAAG | 2 cut(s) | 1130, 3291 |
| AcyI | GRCGYC | 1 cut(s) | 3406 |
| AfaI | GTAC | 6 cut(s) | 327, 468, 1255, 1982, 2681, 3383 |
| AfeI | AGCGCT | 1 cut(s) | 2827 |
| AflIII | ACRYGT | 2 cut(s) | 609, 3142 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3405 |
| AhlI | ACTAGT | 2 cut(s) | 2179, 3428 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 685, 717, 921, 953 |
| AleI | CACNNNNGTG | 1 cut(s) | 2198 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 336, 368, 1562, 1594 |
| Alw21I | GWGCWC | 3 cut(s) | 35, 1217, 3679 |
| Alw26I | GTCTC | 4 cut(s) | 1913, 2201, 3317, 3616 |
| AlwI | GGATC | 4 cut(s) | 453, 816, 1307, 2814 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2018, 2741 |
| Ama87I | CYCGRG | 1 cut(s) | 3615 |
| Aor13HI | TCCGGA | 1 cut(s) | 244 |
| Aor51HI | AGCGCT | 1 cut(s) | 2827 |
| AoxI | GGCC | 8 cut(s) | 286, 779, 1489, 1962, 2613, 3202, 3649, 3716 |
| ApoI | RAATTY | 8 cut(s) | 7, 719, 1088, 1729, 2411, 2912, 3748, 3874 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1073, 1105 |
| AseI | ATTAAT | 2 cut(s) | 1704, 2711 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1237 |
| AspLEI | GCGC | 1 cut(s) | 2828 |
| AspS9I | GGNCC | 3 cut(s) | 569, 2363, 3560 |
| AsuHPI | GGTGA | 4 cut(s) | 490, 709, 728, 2555 |
| AvaI | CYCGRG | 1 cut(s) | 3615 |
| AvaII | GGWCC | 3 cut(s) | 569, 2363, 3560 |
| AxyI | CCTNAGG | 2 cut(s) | 226, 3259 |
| BaeGI | GKGCMC | 1 cut(s) | 2373 |
| BalI | TGGCCA | 2 cut(s) | 288, 1491 |
| BanI | GGYRCC | 1 cut(s) | 3694 |
| BanII | GRGCYC | 3 cut(s) | 35, 692, 2984 |
| BauI | CACGAG | 2 cut(s) | 1242, 3678 |
| BbsI | GAAGAC | 2 cut(s) | 1380, 3198 |
| Bbv12I | GWGCWC | 3 cut(s) | 35, 1217, 3679 |
| BceAI | ACGGC | 1 cut(s) | 3731 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 35, 40, 69, 74, 2360, 2394 |
| BciVI | GTATCC | 1 cut(s) | 2509 |
| BcoDI | GTCTC | 4 cut(s) | 1913, 2201, 3317, 3616 |
| BcuI | ACTAGT | 2 cut(s) | 2179, 3428 |
| BfmI | CTRYAG | 7 cut(s) | 541, 762, 1308, 2019, 2394, 2943, 3483 |
| BfoI | RGCGCY | 1 cut(s) | 2829 |
| BfuI | GTATCC | 1 cut(s) | 2509 |
| BglII | AGATCT | 1 cut(s) | 3804 |
| BlpI | GCTNAGC | 1 cut(s) | 1017 |
| BmcAI | AGTACT | 1 cut(s) | 1255 |
| Bme18I | GGWCC | 3 cut(s) | 569, 2363, 3560 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3405 |
| BmeT110I | CYCGRG | 1 cut(s) | 3615 |
| BmgT120I | GGNCC | 3 cut(s) | 569, 2363, 3560 |
| BmiI | GGNNCC | 7 cut(s) | 689, 912, 1529, 2364, 2981, 3256, 3696 |
| BmrI | ACTGGG | 2 cut(s) | 1482, 1569 |
| BmsI | GCATC | 9 cut(s) | 211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487 |
| BmuI | ACTGGG | 2 cut(s) | 1482, 1569 |
| BpiI | GAAGAC | 2 cut(s) | 1380, 3198 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3661, 3693 |
| BpmI | CTGGAG | 4 cut(s) | 57, 1653, 2632, 3684 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3902 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1017 |
| BpuEI | CTTGAG | 3 cut(s) | 49, 1231, 3688 |
| BsaAI | YACGTR | 2 cut(s) | 772, 1598 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2817 |
| BsaHI | GRCGYC | 1 cut(s) | 3406 |
| BsaI | GGTCTC | 1 cut(s) | 2201 |
| BsaJI | CCNNGG | 8 cut(s) | 153, 437, 740, 1534, 2089, 2225, 2366, 2616 |
| BsaWI | WCCGGW | 1 cut(s) | 244 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1088, 1118, 3020, 3050 |
| Bse118I | RCCGGY | 1 cut(s) | 1964 |
| Bse1I | ACTGG | 6 cut(s) | 1415, 1445, 1477, 1564, 1620, 2254 |
| Bse21I | CCTNAGG | 2 cut(s) | 226, 3259 |
| Bse3DI | GCAATG | 5 cut(s) | 58, 675, 1929, 2593, 3004 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2817 |
| BseAI | TCCGGA | 1 cut(s) | 244 |
| BseDI | CCNNGG | 8 cut(s) | 153, 437, 740, 1534, 2089, 2225, 2366, 2616 |
| BseGI | GGATG | 7 cut(s) | 400, 1801, 1810, 2044, 2658, 2818, 3491 |
| BseJI | GATNNNNATC | 1 cut(s) | 2817 |
| BseMI | GCAATG | 5 cut(s) | 58, 675, 1929, 2593, 3004 |
| BseNI | ACTGG | 6 cut(s) | 1415, 1445, 1477, 1564, 1620, 2254 |
| BseRI | GAGGAG | 3 cut(s) | 194, 197, 514 |
| BseSI | GKGCMC | 1 cut(s) | 2373 |
| BsgI | GTGCAG | 1 cut(s) | 1923 |
| Bsh1236I | CGCG | 2 cut(s) | 1140, 2148 |
| BshFI | GGCC | 8 cut(s) | 288, 781, 1491, 1964, 2615, 3204, 3651, 3718 |
| BshNI | GGYRCC | 1 cut(s) | 3694 |
| BsiHKAI | GWGCWC | 3 cut(s) | 35, 1217, 3679 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3615 |
| BsiSI | CCGG | 3 cut(s) | 245, 1965, 2429 |
| BslFI | GGGAC | 4 cut(s) | 1394, 1489, 2376, 2432 |
| BsmAI | GTCTC | 4 cut(s) | 1913, 2201, 3317, 3616 |
| BsmBI | CGTCTC | 1 cut(s) | 1913 |
| BsmFI | GGGAC | 4 cut(s) | 1394, 1489, 2376, 2432 |
| BsmI | GAATGC | 3 cut(s) | 1239, 2569, 3832 |
| BsnI | GGCC | 8 cut(s) | 288, 781, 1491, 1964, 2615, 3204, 3651, 3718 |
| Bso31I | GGTCTC | 1 cut(s) | 2201 |
| BsoBI | CYCGRG | 1 cut(s) | 3615 |
| Bsp1286I | GDGCHC | 6 cut(s) | 35, 692, 1217, 2373, 2984, 3679 |
| Bsp13I | TCCGGA | 1 cut(s) | 244 |
| Bsp1407I | TGTACA | 2 cut(s) | 2679, 3381 |
| Bsp143I | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1017 |
| Bsp19I | CCATGG | 1 cut(s) | 2616 |
| BspANI | GGCC | 8 cut(s) | 288, 781, 1491, 1964, 2615, 3204, 3651, 3718 |
| BspEI | TCCGGA | 1 cut(s) | 244 |
| BspFNI | CGCG | 2 cut(s) | 1140, 2148 |
| BspHI | TCATGA | 4 cut(s) | 1116, 1198, 1620, 2472 |
| BspLI | GGNNCC | 7 cut(s) | 689, 912, 1529, 2364, 2981, 3256, 3696 |
| BspMAI | CTGCAG | 2 cut(s) | 2398, 3487 |
| BspPI | GGATC | 4 cut(s) | 453, 816, 1307, 2814 |
| BspQI | GCTCTTC | 1 cut(s) | 2341 |
| BspT107I | GGYRCC | 1 cut(s) | 3694 |
| BspTNI | GGTCTC | 1 cut(s) | 2201 |
| BsrBI | CCGCTC | 1 cut(s) | 1204 |
| BsrDI | GCAATG | 5 cut(s) | 58, 675, 1929, 2593, 3004 |
| BsrFI | RCCGGY | 1 cut(s) | 1964 |
| BsrGI | TGTACA | 2 cut(s) | 2679, 3381 |
| BsrI | ACTGG | 6 cut(s) | 1415, 1445, 1477, 1564, 1620, 2254 |
| BssAI | RCCGGY | 1 cut(s) | 1964 |
| BssECI | CCNNGG | 8 cut(s) | 153, 437, 740, 1534, 2089, 2225, 2366, 2616 |
| BssMI | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| BssNI | GRCGYC | 1 cut(s) | 3406 |
| BssSI | CACGAG | 2 cut(s) | 1242, 3678 |
| BssT1I | CCWWGG | 3 cut(s) | 437, 740, 2616 |
| Bst2BI | CACGAG | 2 cut(s) | 1242, 3678 |
| Bst6I | CTCTTC | 2 cut(s) | 2341, 3315 |
| BstACI | GRCGYC | 1 cut(s) | 3406 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1007, 2330, 2741, 3629 |
| BstAUI | TGTACA | 2 cut(s) | 2679, 3381 |
| BstBAI | YACGTR | 2 cut(s) | 772, 1598 |
| BstC8I | GCNNGC | 5 cut(s) | 934, 1142, 2099, 2130, 3310 |
| BstDSI | CCRYGG | 2 cut(s) | 1534, 2616 |
| BstF5I | GGATG | 7 cut(s) | 400, 1801, 1810, 2044, 2658, 2818, 3491 |
| BstFNI | CGCG | 2 cut(s) | 1140, 2148 |
| BstH2I | RGCGCY | 1 cut(s) | 2829 |
| BstHHI | GCGC | 1 cut(s) | 2828 |
| BstKTI | GATC | 8 cut(s) | 448, 753, 811, 1315, 2176, 2809, 3807, 3951 |
| BstMAI | GTCTC | 4 cut(s) | 1913, 2201, 3317, 3616 |
| BstMBI | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| BstNSI | RCATGY | 5 cut(s) | 557, 2105, 3146, 3418, 3901 |
| BstSFI | CTRYAG | 7 cut(s) | 541, 762, 1308, 2019, 2394, 2943, 3483 |
| BstSLI | GKGCMC | 1 cut(s) | 2373 |
| BstSNI | TACGTA | 1 cut(s) | 1598 |
| BstUI | CGCG | 2 cut(s) | 1140, 2148 |
| BstV2I | GAAGAC | 2 cut(s) | 1380, 3198 |
| BstX2I | RGATCY | 4 cut(s) | 445, 808, 1312, 3804 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2200 |
| BstYI | RGATCY | 4 cut(s) | 445, 808, 1312, 3804 |
| Bsu36I | CCTNAGG | 2 cut(s) | 226, 3259 |
| BsuI | GTATCC | 1 cut(s) | 2509 |
| BsuRI | GGCC | 8 cut(s) | 288, 781, 1491, 1964, 2615, 3204, 3651, 3718 |
| BtgI | CCRYGG | 2 cut(s) | 1534, 2616 |
| BtsCI | GGATG | 7 cut(s) | 400, 1801, 1810, 2044, 2658, 2818, 3491 |
| BtsI | GCAGTG | 3 cut(s) | 2966, 3100, 3300 |
| Cac8I | GCNNGC | 5 cut(s) | 934, 1142, 2099, 2130, 3310 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2018, 2741 |
| CciI | TCATGA | 4 cut(s) | 1116, 1198, 1620, 2472 |
| CfoI | GCGC | 1 cut(s) | 2828 |
| Cfr10I | RCCGGY | 1 cut(s) | 1964 |
| Cfr13I | GGNCC | 3 cut(s) | 569, 2363, 3560 |
| Csp6I | GTAC | 6 cut(s) | 326, 467, 1254, 1981, 2680, 3382 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3573, 3608 |
| CviQI | GTAC | 6 cut(s) | 326, 467, 1254, 1981, 2680, 3382 |
| DpnI | GATC | 8 cut(s) | 447, 752, 810, 1314, 2175, 2808, 3806, 3950 |
| DpnII | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3405 |
| EaeI | YGGCCR | 4 cut(s) | 286, 1489, 1962, 3716 |
| Eam1104I | CTCTTC | 2 cut(s) | 2341, 3315 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3405 |
| EarI | CTCTTC | 2 cut(s) | 2341, 3315 |
| Ecl136II | GAGCTC | 1 cut(s) | 33 |
| Eco105I | TACGTA | 1 cut(s) | 1598 |
| Eco130I | CCWWGG | 3 cut(s) | 437, 740, 2616 |
| Eco24I | GRGCYC | 3 cut(s) | 35, 692, 2984 |
| Eco31I | GGTCTC | 1 cut(s) | 2201 |
| Eco47I | GGWCC | 3 cut(s) | 569, 2363, 3560 |
| Eco47III | AGCGCT | 1 cut(s) | 2827 |
| Eco53kI | GAGCTC | 1 cut(s) | 33 |
| Eco57I | CTGAAG | 2 cut(s) | 1130, 3291 |
| Eco81I | CCTNAGG | 2 cut(s) | 226, 3259 |
| Eco88I | CYCGRG | 1 cut(s) | 3615 |
| EcoICRI | GAGCTC | 1 cut(s) | 33 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2363 |
| EcoT14I | CCWWGG | 3 cut(s) | 437, 740, 2616 |
| EcoT22I | ATGCAT | 2 cut(s) | 3116, 3420 |
| EcoT38I | GRGCYC | 3 cut(s) | 35, 692, 2984 |
| ErhI | CCWWGG | 3 cut(s) | 437, 740, 2616 |
| Esp3I | CGTCTC | 1 cut(s) | 1913 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 937, 969 |
| FaqI | GGGAC | 4 cut(s) | 1394, 1489, 2376, 2432 |
| FauI | CCCGC | 2 cut(s) | 1419, 2708 |
| FauNDI | CATATG | 2 cut(s) | 1030, 2722 |
| FokI | GGATG | 7 cut(s) | 407, 1808, 1817, 2031, 2665, 2825, 3478 |
| FriOI | GRGCYC | 3 cut(s) | 35, 692, 2984 |
| GlaI | GCGC | 1 cut(s) | 2827 |
| GsuI | CTGGAG | 4 cut(s) | 57, 1653, 2632, 3684 |
| HaeII | RGCGCY | 1 cut(s) | 2829 |
| HaeIII | GGCC | 8 cut(s) | 288, 781, 1491, 1964, 2615, 3204, 3651, 3718 |
| HapII | CCGG | 3 cut(s) | 245, 1965, 2429 |
| HhaI | GCGC | 1 cut(s) | 2828 |
| Hin1I | GRCGYC | 1 cut(s) | 3406 |
| Hin6I | GCGC | 1 cut(s) | 2826 |
| HinP1I | GCGC | 1 cut(s) | 2826 |
| HindIII | AAGCTT | 5 cut(s) | 239, 1340, 1956, 3306, 3575 |
| HinfI | GANTC | 7 cut(s) | 296, 419, 1817, 2635, 2786, 3169, 3817 |
| HpaII | CCGG | 3 cut(s) | 245, 1965, 2429 |
| HphI | GGTGA | 4 cut(s) | 490, 709, 728, 2555 |
| Hpy166II | GTNNAC | 5 cut(s) | 413, 769, 2836, 3382, 3384 |
| Hpy8I | GTNNAC | 5 cut(s) | 413, 769, 2836, 3382, 3384 |
| Hpy99I | CGWCG | 2 cut(s) | 996, 2246 |
| HpyCH4IV | ACGT | 8 cut(s) | 313, 609, 771, 1597, 2338, 2554, 3244, 3406 |
| HpySE526I | ACGT | 8 cut(s) | 313, 609, 771, 1597, 2338, 2554, 3244, 3406 |
| Hsp92I | GRCGYC | 1 cut(s) | 3406 |
| HspAI | GCGC | 1 cut(s) | 2826 |
| Kpn2I | TCCGGA | 1 cut(s) | 244 |
| Kzo9I | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| LguI | GCTCTTC | 1 cut(s) | 2341 |
| LmnI | GCTCC | 9 cut(s) | 687, 742, 1206, 1533, 1926, 2661, 2979, 3063, 3466 |
| LweI | GCATC | 9 cut(s) | 211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487 |
| MaeII | ACGT | 8 cut(s) | 313, 609, 771, 1597, 2338, 2554, 3244, 3406 |
| MalI | GATC | 8 cut(s) | 447, 752, 810, 1314, 2175, 2808, 3806, 3950 |
| MbiI | CCGCTC | 1 cut(s) | 1204 |
| MboI | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| MfeI | CAATTG | 1 cut(s) | 783 |
| MflI | RGATCY | 4 cut(s) | 445, 808, 1312, 3804 |
| MhlI | GDGCHC | 6 cut(s) | 35, 692, 1217, 2373, 2984, 3679 |
| MlsI | TGGCCA | 2 cut(s) | 288, 1491 |
| MluNI | TGGCCA | 2 cut(s) | 288, 1491 |
| MlyI | GAGTC | 4 cut(s) | 428, 2644, 2780, 3178 |
| MmeI | TCCRAC | 5 cut(s) | 156, 402, 782, 2540, 3085 |
| Mox20I | TGGCCA | 2 cut(s) | 288, 1491 |
| Mph1103I | ATGCAT | 2 cut(s) | 3116, 3420 |
| MroI | TCCGGA | 1 cut(s) | 244 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1237 |
| MscI | TGGCCA | 2 cut(s) | 288, 1491 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2198 |
| Msp20I | TGGCCA | 2 cut(s) | 288, 1491 |
| MspA1I | CMGCKG | 3 cut(s) | 2330, 2738, 3786 |
| MspI | CCGG | 3 cut(s) | 245, 1965, 2429 |
| MunI | CAATTG | 1 cut(s) | 783 |
| Mva1269I | GAATGC | 3 cut(s) | 1239, 2569, 3832 |
| MvnI | CGCG | 2 cut(s) | 1140, 2148 |
| NcoI | CCATGG | 1 cut(s) | 2616 |
| NdeI | CATATG | 2 cut(s) | 1030, 2722 |
| NdeII | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| NlaIV | GGNNCC | 7 cut(s) | 689, 912, 1529, 2364, 2981, 3256, 3696 |
| NmuCI | GTSAC | 5 cut(s) | 697, 947, 979, 1555, 3520 |
| NsiI | ATGCAT | 2 cut(s) | 3116, 3420 |
| NspI | RCATGY | 5 cut(s) | 557, 2105, 3146, 3418, 3901 |
| OliI | CACNNNNGTG | 1 cut(s) | 2198 |
| PaeR7I | CTCGAG | 1 cut(s) | 3615 |
| PagI | TCATGA | 4 cut(s) | 1116, 1198, 1620, 2472 |
| PciI | ACATGT | 1 cut(s) | 3142 |
| PciSI | GCTCTTC | 1 cut(s) | 2341 |
| PctI | GAATGC | 3 cut(s) | 1239, 2569, 3832 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1237 |
| PfeI | GAWTC | 3 cut(s) | 296, 1817, 3817 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2803, 3557 |
| PfoI | TCCNGGA | 3 cut(s) | 1315, 2648, 3843 |
| PleI | GAGTC | 4 cut(s) | 427, 2643, 2780, 3177 |
| PpsI | GAGTC | 4 cut(s) | 427, 2643, 2780, 3177 |
| Ppu21I | YACGTR | 2 cut(s) | 772, 1598 |
| PpuMI | RGGWCCY | 1 cut(s) | 2363 |
| PscI | ACATGT | 1 cut(s) | 3142 |
| PshBI | ATTAAT | 2 cut(s) | 1704, 2711 |
| Psp124BI | GAGCTC | 1 cut(s) | 35 |
| Psp1406I | AACGTT | 1 cut(s) | 2554 |
| Psp5II | RGGWCCY | 1 cut(s) | 2363 |
| PspN4I | GGNNCC | 7 cut(s) | 689, 912, 1529, 2364, 2981, 3256, 3696 |
| PspPI | GGNCC | 3 cut(s) | 569, 2363, 3560 |
| PspPPI | RGGWCCY | 1 cut(s) | 2363 |
| PstI | CTGCAG | 2 cut(s) | 2398, 3487 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2018, 2741 |
| PsuI | RGATCY | 4 cut(s) | 445, 808, 1312, 3804 |
| PvuII | CAGCTG | 2 cut(s) | 2330, 3786 |
| RsaI | GTAC | 6 cut(s) | 327, 468, 1255, 1982, 2681, 3383 |
| RsaNI | GTAC | 6 cut(s) | 326, 467, 1254, 1981, 2680, 3382 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2198 |
| SacI | GAGCTC | 1 cut(s) | 35 |
| SapI | GCTCTTC | 1 cut(s) | 2341 |
| Sau3AI | GATC | 8 cut(s) | 445, 750, 808, 1312, 2173, 2806, 3804, 3948 |
| Sau96I | GGNCC | 3 cut(s) | 569, 2363, 3560 |
| ScaI | AGTACT | 1 cut(s) | 1255 |
| SchI | GAGTC | 4 cut(s) | 428, 2644, 2780, 3178 |
| SduI | GDGCHC | 6 cut(s) | 35, 692, 1217, 2373, 2984, 3679 |
| SfaNI | GCATC | 9 cut(s) | 211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487 |
| SfcI | CTRYAG | 7 cut(s) | 541, 762, 1308, 2019, 2394, 2943, 3483 |
| Sfr274I | CTCGAG | 1 cut(s) | 3615 |
| SinI | GGWCC | 3 cut(s) | 569, 2363, 3560 |
| SlaI | CTCGAG | 1 cut(s) | 3615 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2198 |
| SmlI | CTYRAG | 4 cut(s) | 28, 1210, 3615, 3667 |
| SmoI | CTYRAG | 4 cut(s) | 28, 1210, 3615, 3667 |
| SnaBI | TACGTA | 1 cut(s) | 1598 |
| SpeI | ACTAGT | 2 cut(s) | 2179, 3428 |
| SspI | AATATT | 3 cut(s) | 1131, 2439, 2488 |
| SstI | GAGCTC | 1 cut(s) | 35 |
| StyI | CCWWGG | 3 cut(s) | 437, 740, 2616 |
| TaiI | ACGT | 8 cut(s) | 316, 612, 774, 1600, 2341, 2557, 3247, 3409 |
| TaqI | TCGA | 4 cut(s) | 961, 2176, 2244, 3616 |
| TatI | WGTACW | 6 cut(s) | 325, 466, 1253, 1980, 2679, 3381 |
| TauI | GCSGC | 3 cut(s) | 2135, 2741, 3925 |
| TfiI | GAWTC | 3 cut(s) | 296, 1817, 3817 |
| TseFI | GTSAC | 5 cut(s) | 697, 947, 979, 1555, 3520 |
| Tsp45I | GTSAC | 5 cut(s) | 697, 947, 979, 1555, 3520 |
| TspGWI | ACGGA | 3 cut(s) | 1232, 1493, 3453 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2803, 3557 |
| VpaK11BI | GGWCC | 3 cut(s) | 569, 2363, 3560 |
| VspI | ATTAAT | 2 cut(s) | 1704, 2711 |
| XapI | RAATTY | 8 cut(s) | 7, 719, 1088, 1729, 2411, 2912, 3748, 3874 |
| XceI | RCATGY | 5 cut(s) | 557, 2105, 3146, 3418, 3901 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1366, 2051 |
| XhoI | CTCGAG | 1 cut(s) | 3615 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1237 |
| ZraI | GACGTC | 1 cut(s) | 3407 |
| ZrmI | AGTACT | 1 cut(s) | 1255 |
| Zsp2I | ATGCAT | 2 cut(s) | 3116, 3420 |