Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Forward (+)

5731295 .. 5733545

Gene Structure 2251 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00083010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGCTGCCTGCAGCAGCTCAGCGTCATGTATTTAAAAAAGTCAAGGAGGGGGAACGACTTCTTGTTGTGGCGACTAATGTGGCTGAAACCTCTTTAACTATACCTGGGATAAAGTATGTAGTCGACACTGGAAAAGTGAAAGTGAAGGACTACAATTTCTCAAATGGCATGGAAACATATAAAGTACAGTGGATTAGTAAGGCATCAGCTATTCAACGCGAAGGAAGAGCTGGAAGAACAGGGCCTGGCCACTGTTTCCGCTTGTATTCTTCTGCAGTATATGACAATGAGTTTCTTGACTGTTCCGAAGCTGAAATATCCAAAGTCCCTGTTGATGGTGTATCCAAATTTCAGTTTCCTACTCCTCCAAATGGTGATACTATTGATGAAGCAGAGGATCGTTTGAAGCTCCTCAAAGCACTTGATAGCAATGGAGAGGTGACACCTCTAGGGAAGGCCATGGCTTGTTACCCCATAAGTCCTTGCCAATCTAAGATGCTCCTTACGGTCATTGAAATCCTGAATATGAAGCAAAGTTATTCCCGAGCTAATTTAGTGCTAGCATATGCAGTTGCAGCAGCTGCAGCTTTGAGTGTGTCTAATCCTTTTGACAGTCAATTTGAAGATCGTCACATAAAAAACTTTGACTTAGGCTATGAACTTTCCAAGAGCCAAATGGGTTTCTGCAAGGAAAATGCTCTACACCCGGAAACCATGGAGGAAATATCCAAGCTGAGAAAGCAGCTCCTCAAACTTGTCTTTAATCCAAATGGTGTTTCTGGAGGTGTGAAGGAATTTTTGTGGATCTATGGTAGTCAGAAAGATGTTGAAACTGTTTGGAGGGATGGTGAGAAGCTTCTCTCAGTCAATGAGGAAGAGCTCTTACGCCAAGCTATCTGTGCTGGTTGGATAGATAGGGTTGCCAAACGCATTGAAGGAAGTGCATATCAGGCCTGCATGGTCAAAGAGACTGTGTTCCTCCACTGGCAGTCACCTGTTTCTAAAATTGCTACTGAGTTTTTGGTGTACAGTGAGTTGATCCAGACAAAGAAACCTTTCGTGTACGGCATTACTTGTGTCAGTCCAGATTGGCTCGTCAAGTATGGTCTGCTTCCCATGTATGATCGGCGCTTGTGCACTTTTTCAAAAGAAGCTCGGCCTTTCTATGACCGTCAAACTGACAAACTATTATATATTGTCACTCCAACCTTTGGTCGTGTTCAACGGTGGGAGCTTCCACAACATTCTTTGCCAATTAGTAATTTTGATCATACAGGCGCAGTTCAAGCTTTTGCTTTTGCTTTACTCGATGGCAAGGTTTTGCATTGCCTAAGACCTCTGCGTGAGTCCATGGCAGAACGTTCTCACACTGTTTTAAGGCCAGAGGCAGCAGGTCGAGAACTGGTTCATAATCTCTTGGCGAAGCTGGGGAAGAAAAAGATAGTCAGCCGTGCTATGTTGATAGAGGTGTGGAAGGAAAATCCCACTGAATTGTATCCGGAAATCCGGAGCTGGTTTAAGGAGAGTTTCCACCAGAAGTTTAGGGAAATTTGGGTCAAGATGCTTTATGAAGCCCTTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

527

Amino Acids

59.9

Weight (kDa)

8.76

Isoelectric Point (pI)

40.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	3 - 81	1.8e-08	Helicase conserved C-terminal domain
WHD_HA2	PF04408	131 - 159	3.4e-07	Helicase associated domain (HA2), winged-helix
OB_NTP_bind	PF07717	295 - 368	2.3e-16	Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C	PF23362	444 - 521	4.7e-14	DEAH helicase DHX37 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1382
AccB7I	CCANNNNNTGG	1 cut(s)	1211
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	1 cut(s)	219
AccIII	TCCGGA	2 cut(s)	1498, 1506
AciI	CCGC	1 cut(s)	259
AclI	AACGTT	1 cut(s)	1360
AclWI	GGATC	3 cut(s)	405, 812, 1033
AcoI	YGGCCR	1 cut(s)	247
AcsI	RAATTY	3 cut(s)	347, 794, 1548
AfaI	GTAC	3 cut(s)	186, 1028, 1064
AfiI	CCNNNNNNNGG	3 cut(s)	335, 371, 1211
AgsI	TTSAA	9 cut(s)	215, 406, 515, 623, 830, 935, 1146, 1223, 1286
AjnI	CCWGG	2 cut(s)	103, 244
AjuI	GAANNNNNNNTTGG	2 cut(s)	760, 792
Alw21I	GWGCWC	2 cut(s)	882, 1139
Alw26I	GTCTC	1 cut(s)	962
Alw44I	GTGCAC	1 cut(s)	1135
AlwI	GGATC	3 cut(s)	405, 812, 1033
AlwNI	CAGNNNCTG	2 cut(s)	245, 581
Ama87I	CYCGRG	1 cut(s)	543
Aor13HI	TCCGGA	2 cut(s)	1498, 1506
AoxI	GGCC	6 cut(s)	242, 247, 456, 951, 1157, 1379
ApaLI	GTGCAC	1 cut(s)	1135
ApeKI	GCWGC	9 cut(s)	4, 11, 14, 575, 578, 581, 584, 742, 1388
ApoI	RAATTY	3 cut(s)	347, 794, 1548
ArsI	GACNNNNNNTTYG	2 cut(s)	602, 634
Asp700I	GAANNNNTTC	3 cut(s)	57, 1055, 1404
AspLEI	GCGC	2 cut(s)	1131, 1280
AspS9I	GGNCC	1 cut(s)	242
AsuC2I	CCSGG	1 cut(s)	707
AsuHPI	GGTGA	4 cut(s)	386, 451, 860, 984
AsuNHI	GCTAGC	1 cut(s)	559
AvaI	CYCGRG	1 cut(s)	543
BaeGI	GKGCMC	1 cut(s)	1139
BalI	TGGCCA	1 cut(s)	249
BanII	GRGCYC	1 cut(s)	882
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
Bbv12I	GWGCWC	2 cut(s)	882, 1139
BbvI	GCAGC	8 cut(s)	23, 26, 568, 587, 590, 596, 754, 1400
BccI	CCATC	3 cut(s)	329, 839, 1304
BceAI	ACGGC	2 cut(s)	1081, 1434
BciT130I	CCWGG	2 cut(s)	105, 246
BciVI	GTATCC	2 cut(s)	352, 1506
BclI	TGATCA	1 cut(s)	1267
BcnI	CCSGG	1 cut(s)	707
BcoDI	GTCTC	1 cut(s)	962
BfaI	CTAG	2 cut(s)	449, 560
BfmI	CTRYAG	3 cut(s)	9, 273, 582
BfoI	RGCGCY	1 cut(s)	1132
BfuAI	ACCTGC	1 cut(s)	1382
BfuI	GTATCC	2 cut(s)	352, 1506
BisI	GCNGC	9 cut(s)	5, 12, 15, 576, 579, 582, 585, 743, 1389
BlpI	GCTNAGC	1 cut(s)	18
BlsI	GCNGC	9 cut(s)	6, 13, 16, 577, 580, 583, 586, 744, 1390
Bme1390I	CCNGG	3 cut(s)	105, 246, 707
BmeT110I	CYCGRG	1 cut(s)	543
BmgT120I	GGNCC	1 cut(s)	242
BmrFI	CCNGG	3 cut(s)	105, 246, 707
BmsI	GCATC	3 cut(s)	212, 486, 1551
BmtI	GCTAGC	1 cut(s)	563
BpmI	CTGGAG	1 cut(s)	801
Bpu1102I	GCTNAGC	1 cut(s)	18
BpuMI	CCSGG	1 cut(s)	707
BsaJI	CCNNGG	4 cut(s)	104, 459, 714, 1350
BsaWI	WCCGGW	2 cut(s)	1498, 1506
BsaXI	ACNNNNNCTCC	2 cut(s)	832, 862
Bsc4I	CCNNNNNNNGG	3 cut(s)	335, 371, 1211
Bse1I	ACTGG	3 cut(s)	133, 989, 1407
Bse3DI	GCAATG	2 cut(s)	436, 1324
BseAI	TCCGGA	2 cut(s)	1498, 1506
BseBI	CCWGG	2 cut(s)	105, 246
BseDI	CCNNGG	4 cut(s)	104, 459, 714, 1350
BseGI	GGATG	1 cut(s)	850
BseLI	CCNNNNNNNGG	3 cut(s)	335, 371, 1211
BseMI	GCAATG	2 cut(s)	436, 1324
BseMII	CTCAG	4 cut(s)	32, 725, 876, 1005
BseNI	ACTGG	3 cut(s)	133, 989, 1407
BseRI	GAGGAG	3 cut(s)	354, 401, 737
BseSI	GKGCMC	1 cut(s)	1139
BseXI	GCAGC	8 cut(s)	23, 26, 568, 587, 590, 596, 754, 1400
BseYI	CCCAGC	1 cut(s)	1426
Bsh1236I	CGCG	1 cut(s)	219
BshFI	GGCC	6 cut(s)	244, 249, 458, 953, 1159, 1381
BsiHKAI	GWGCWC	2 cut(s)	882, 1139
BsiHKCI	CYCGRG	1 cut(s)	543
BsiSI	CCGG	3 cut(s)	707, 1499, 1507
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	3 cut(s)	335, 371, 1211
BsmAI	GTCTC	1 cut(s)	962
BsmFI	GGGAC	1 cut(s)	311
BsnI	GGCC	6 cut(s)	244, 249, 458, 953, 1159, 1381
BsoBI	CYCGRG	1 cut(s)	543
Bsp1286I	GDGCHC	2 cut(s)	882, 1139
Bsp13I	TCCGGA	2 cut(s)	1498, 1506
Bsp1407I	TGTACA	1 cut(s)	1026
Bsp143I	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
Bsp1720I	GCTNAGC	1 cut(s)	18
Bsp19I	CCATGG	3 cut(s)	459, 714, 1350
BspACI	CCGC	1 cut(s)	259
BspANI	GGCC	6 cut(s)	244, 249, 458, 953, 1159, 1381
BspCNI	CTCAG	4 cut(s)	31, 726, 875, 1006
BspEI	TCCGGA	2 cut(s)	1498, 1506
BspFNI	CGCG	1 cut(s)	219
BspMAI	CTGCAG	3 cut(s)	13, 277, 586
BspMI	ACCTGC	1 cut(s)	1382
BspOI	GCTAGC	1 cut(s)	563
BspPI	GGATC	3 cut(s)	405, 812, 1033
BspQI	GCTCTTC	2 cut(s)	220, 870
BsrDI	GCAATG	2 cut(s)	436, 1324
BsrGI	TGTACA	1 cut(s)	1026
BsrI	ACTGG	3 cut(s)	133, 989, 1407
BssECI	CCNNGG	4 cut(s)	104, 459, 714, 1350
BssMI	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
BssT1I	CCWWGG	3 cut(s)	459, 714, 1350
Bst2UI	CCWGG	2 cut(s)	105, 246
Bst6I	CTCTTC	2 cut(s)	220, 870
BstAPI	GCANNNNNTGC	1 cut(s)	581
BstAUI	TGTACA	1 cut(s)	1026
BstC8I	GCNNGC	3 cut(s)	9, 561, 955
BstDEI	CTNAG	7 cut(s)	18, 492, 649, 734, 862, 1014, 1331
BstDSI	CCRYGG	3 cut(s)	459, 714, 1350
BstF5I	GGATG	1 cut(s)	850
BstFNI	CGCG	1 cut(s)	219
BstH2I	RGCGCY	1 cut(s)	1132
BstHHI	GCGC	2 cut(s)	1131, 1280
BstKTI	GATC	6 cut(s)	400, 628, 807, 1041, 1126, 1270
BstMAI	GTCTC	1 cut(s)	962
BstMBI	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
BstMWI	GCNNNNNNNGC	7 cut(s)	575, 581, 584, 739, 899, 950, 1286
BstNI	CCWGG	2 cut(s)	105, 246
BstSCI	CCNGG	3 cut(s)	103, 244, 705
BstSFI	CTRYAG	3 cut(s)	9, 273, 582
BstSLI	GKGCMC	1 cut(s)	1139
BstUI	CGCG	1 cut(s)	219
BstV1I	GCAGC	8 cut(s)	23, 26, 568, 587, 590, 596, 754, 1400
BstX2I	RGATCY	1 cut(s)	804
BstYI	RGATCY	1 cut(s)	804
BsuI	GTATCC	2 cut(s)	352, 1506
BsuRI	GGCC	6 cut(s)	244, 249, 458, 953, 1159, 1381
BtgI	CCRYGG	3 cut(s)	459, 714, 1350
BtsCI	GGATG	1 cut(s)	850
BtsIMutI	CAGTG	7 cut(s)	126, 194, 250, 982, 1036, 1368, 1485
BveI	ACCTGC	1 cut(s)	1382
Cac8I	GCNNGC	3 cut(s)	9, 561, 955
CaiI	CAGNNNCTG	2 cut(s)	245, 581
CfoI	GCGC	2 cut(s)	1131, 1280
Cfr13I	GGNCC	1 cut(s)	242
CseI	GACGC	1 cut(s)	11
Csp6I	GTAC	3 cut(s)	185, 1027, 1063
CviAII	CATG	7 cut(s)	26, 169, 460, 715, 958, 1117, 1351
CviQI	GTAC	3 cut(s)	185, 1027, 1063
DdeI	CTNAG	7 cut(s)	18, 492, 649, 734, 862, 1014, 1331
DpnI	GATC	6 cut(s)	399, 627, 806, 1040, 1125, 1269
DpnII	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
DraI	TTTAAA	1 cut(s)	34
EaeI	YGGCCR	1 cut(s)	247
Eam1104I	CTCTTC	2 cut(s)	220, 870
EarI	CTCTTC	2 cut(s)	220, 870
Ecl136II	GAGCTC	1 cut(s)	880
Eco130I	CCWWGG	3 cut(s)	459, 714, 1350
Eco147I	AGGCCT	1 cut(s)	953
Eco24I	GRGCYC	1 cut(s)	882
Eco53kI	GAGCTC	1 cut(s)	880
Eco88I	CYCGRG	1 cut(s)	543
EcoICRI	GAGCTC	1 cut(s)	880
EcoO109I	RGGNCCY	1 cut(s)	242
EcoRII	CCWGG	2 cut(s)	103, 244
EcoT14I	CCWWGG	3 cut(s)	459, 714, 1350
EcoT38I	GRGCYC	1 cut(s)	882
ErhI	CCWWGG	3 cut(s)	459, 714, 1350
FaeI	CATG	7 cut(s)	29, 172, 463, 718, 961, 1120, 1354
FaqI	GGGAC	1 cut(s)	311
FatI	CATG	7 cut(s)	25, 168, 459, 714, 957, 1116, 1350
FauNDI	CATATG	1 cut(s)	565
FbaI	TGATCA	1 cut(s)	1267
FblI	GTMKAC	1 cut(s)	123
Fnu4HI	GCNGC	9 cut(s)	5, 12, 15, 576, 579, 582, 585, 743, 1389
FokI	GGATG	1 cut(s)	857
FriOI	GRGCYC	1 cut(s)	882
Fsp4HI	GCNGC	9 cut(s)	5, 12, 15, 576, 579, 582, 585, 743, 1389
FspBI	CTAG	2 cut(s)	449, 560
GlaI	GCGC	2 cut(s)	1130, 1279
GluI	GCNGC	9 cut(s)	5, 12, 15, 576, 579, 582, 585, 743, 1389
GsaI	CCCAGC	1 cut(s)	1430
GsuI	CTGGAG	1 cut(s)	801
HaeII	RGCGCY	1 cut(s)	1132
HaeIII	GGCC	6 cut(s)	244, 249, 458, 953, 1159, 1381
HapII	CCGG	3 cut(s)	707, 1499, 1507
HgaI	GACGC	1 cut(s)	11
HhaI	GCGC	2 cut(s)	1131, 1280
Hin1II	CATG	7 cut(s)	29, 172, 463, 718, 961, 1120, 1354
Hin6I	GCGC	2 cut(s)	1129, 1278
HinP1I	GCGC	2 cut(s)	1129, 1278
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HindIII	AAGCTT	2 cut(s)	854, 1287
HinfI	GANTC	1 cut(s)	1346
HpaII	CCGG	3 cut(s)	707, 1499, 1507
HphI	GGTGA	4 cut(s)	386, 451, 860, 984
Hpy166II	GTNNAC	4 cut(s)	124, 1027, 1063, 1137
Hpy188I	TCNGA	2 cut(s)	307, 819
Hpy8I	GTNNAC	4 cut(s)	124, 1027, 1063, 1137
HpyAV	CCTTC	6 cut(s)	139, 215, 448, 784, 929, 1468
HpyCH4IV	ACGT	1 cut(s)	1360
HpyF10VI	GCNNNNNNNGC	7 cut(s)	575, 581, 584, 739, 899, 950, 1286
HpyF3I	CTNAG	7 cut(s)	18, 492, 649, 734, 862, 1014, 1331
HpySE526I	ACGT	1 cut(s)	1360
Hsp92II	CATG	7 cut(s)	29, 172, 463, 718, 961, 1120, 1354
HspAI	GCGC	2 cut(s)	1129, 1278
Kpn2I	TCCGGA	2 cut(s)	1498, 1506
Ksp22I	TGATCA	1 cut(s)	1267
Kzo9I	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
LguI	GCTCTTC	2 cut(s)	220, 870
LmnI	GCTCC	5 cut(s)	414, 504, 750, 1231, 1509
Lsp1109I	GCAGC	8 cut(s)	23, 26, 568, 587, 590, 596, 754, 1400
LweI	GCATC	3 cut(s)	212, 486, 1551
MaeI	CTAG	2 cut(s)	449, 560
MaeII	ACGT	1 cut(s)	1360
MaeIII	GTNAC	5 cut(s)	439, 467, 629, 990, 1198
MalI	GATC	6 cut(s)	399, 627, 806, 1040, 1125, 1269
MboI	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
MboII	GAAGA	6 cut(s)	237, 246, 261, 635, 887, 1444
MflI	RGATCY	1 cut(s)	804
MhlI	GDGCHC	2 cut(s)	882, 1139
MlsI	TGGCCA	1 cut(s)	249
MluNI	TGGCCA	1 cut(s)	249
MlyI	GAGTC	1 cut(s)	1355
MmeI	TCCRAC	2 cut(s)	887, 1229
Mox20I	TGGCCA	1 cut(s)	249
MroI	TCCGGA	2 cut(s)	1498, 1506
MroXI	GAANNNNTTC	3 cut(s)	57, 1055, 1404
MscI	TGGCCA	1 cut(s)	249
MseI	TTAA	5 cut(s)	33, 95, 762, 1376, 1518
Msp20I	TGGCCA	1 cut(s)	249
MspA1I	CMGCKG	1 cut(s)	581
MspI	CCGG	3 cut(s)	707, 1499, 1507
MspR9I	CCNGG	3 cut(s)	105, 246, 707
MvaI	CCWGG	2 cut(s)	105, 246
MvnI	CGCG	1 cut(s)	219
MwoI	GCNNNNNNNGC	7 cut(s)	575, 581, 584, 739, 899, 950, 1286
NciI	CCSGG	1 cut(s)	707
NcoI	CCATGG	3 cut(s)	459, 714, 1350
NdeI	CATATG	1 cut(s)	565
NdeII	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
NheI	GCTAGC	1 cut(s)	559
NlaIII	CATG	7 cut(s)	29, 172, 463, 718, 961, 1120, 1354
NmeAIII	GCCGAG	1 cut(s)	1135
NmuCI	GTSAC	4 cut(s)	439, 629, 990, 1198
PceI	AGGCCT	1 cut(s)	953
PciSI	GCTCTTC	2 cut(s)	220, 870
PdmI	GAANNNNTTC	3 cut(s)	57, 1055, 1404
PflMI	CCANNNNNTGG	1 cut(s)	1211
PkrI	GCNGC	9 cut(s)	6, 13, 16, 577, 580, 583, 586, 744, 1390
PleI	GAGTC	1 cut(s)	1354
PpsI	GAGTC	1 cut(s)	1354
Psp124BI	GAGCTC	1 cut(s)	882
Psp1406I	AACGTT	1 cut(s)	1360
Psp6I	CCWGG	2 cut(s)	103, 244
PspFI	CCCAGC	1 cut(s)	1426
PspGI	CCWGG	2 cut(s)	103, 244
PspPI	GGNCC	1 cut(s)	242
PstI	CTGCAG	3 cut(s)	13, 277, 586
PstNI	CAGNNNCTG	2 cut(s)	245, 581
PsuI	RGATCY	1 cut(s)	804
PvuII	CAGCTG	1 cut(s)	581
RsaI	GTAC	3 cut(s)	186, 1028, 1064
RsaNI	GTAC	3 cut(s)	185, 1027, 1063
SacI	GAGCTC	1 cut(s)	882
SalI	GTCGAC	1 cut(s)	122
SapI	GCTCTTC	2 cut(s)	220, 870
SaqAI	TTAA	5 cut(s)	33, 95, 762, 1376, 1518
SatI	GCNGC	9 cut(s)	5, 12, 15, 576, 579, 582, 585, 743, 1389
Sau3AI	GATC	6 cut(s)	397, 625, 804, 1038, 1123, 1267
Sau96I	GGNCC	1 cut(s)	242
SchI	GAGTC	1 cut(s)	1355
ScrFI	CCNGG	3 cut(s)	105, 246, 707
SduI	GDGCHC	2 cut(s)	882, 1139
SfaNI	GCATC	3 cut(s)	212, 486, 1551
SfcI	CTRYAG	3 cut(s)	9, 273, 582
SseBI	AGGCCT	1 cut(s)	953
SsiI	CCGC	1 cut(s)	259
SspMI	CTAG	2 cut(s)	449, 560
SstI	GAGCTC	1 cut(s)	882
StuI	AGGCCT	1 cut(s)	953
StyD4I	CCNGG	3 cut(s)	103, 244, 705
StyI	CCWWGG	3 cut(s)	459, 714, 1350
TaiI	ACGT	1 cut(s)	1363
TaqI	TCGA	3 cut(s)	123, 1308, 1396
TatI	WGTACW	2 cut(s)	184, 1026
Tru1I	TTAA	5 cut(s)	33, 95, 762, 1376, 1518
Tru9I	TTAA	5 cut(s)	33, 95, 762, 1376, 1518
TscAI	CASTG	7 cut(s)	133, 194, 257, 989, 1036, 1375, 1492
TseFI	GTSAC	4 cut(s)	439, 629, 990, 1198
TseI	GCWGC	9 cut(s)	4, 11, 14, 575, 578, 581, 584, 742, 1388
Tsp45I	GTSAC	4 cut(s)	439, 629, 990, 1198
TspDTI	ATGAA	5 cut(s)	402, 542, 672, 1397, 1584
TspRI	CASTG	7 cut(s)	133, 194, 257, 989, 1036, 1375, 1492
Van91I	CCANNNNNTGG	1 cut(s)	1211
VneI	GTGCAC	1 cut(s)	1135
XapI	RAATTY	3 cut(s)	347, 794, 1548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	673
XmiI	GTMKAC	1 cut(s)	123
XmnI	GAANNNNTTC	3 cut(s)	57, 1055, 1404
XspI	CTAG	2 cut(s)	449, 560

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.