Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Forward (+)

37422798 .. 37429162

Gene Structure 6365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G028070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3678 bp

ATGAACATGTTGCCAGACAAGAGAAGGAGTAATAATAAAAGAAAGCGAACCAATGAGGAATGTCACAAACGACTTAGAGAAACCCAGAGGATGATGAGCACATCTCACAAAAGGAAGCTCAACAAGTTGAAGGAGGAGAAGGAAAACGACCTTGTGCTATCAAAAAGCGTTGAGGCGTTAGAGAAATATAAGATTCCAGAGGCTGCACGGGCTCTTCTCCGGTCATCACGAAATATTGGCCAGGTTGAATCCAGAAAGGTTAAACGCAGCAAAGGTTTACTGCACTTGAAACTTACATCCCATGCTTCCAGTGAAAGTGAACCTGAGTCGGAAATAACTCAATCAACACAGGATCAATCGTTAATGGTACAAAAGAACACGTCTACTTGTTCGGACTCCTCTACAATGCTACTTTGTGGCACAAGGCCTCCTGATATTCGTAACAAGTCTTTACCAGAAGATGGAACTTGTAGCATGGATCATAACTGGAGTGAAAGCATAAACTTCATGGATAAAGTAGAAGAGAGTCCTGAGTCAAGTTACAGCTCAAAGCTTCCTGCACCAAGTATTCTGAAAGATCCGAAAAATAGGCGGCATGTATCAAGACCTCCTGAAGTGGTAAAGGCAAGGAGTGATATTCCTATTATAATGATGGAGCAAGAGATAATGGAGGCCATAAATGAAAATTCCACTGTTATTATACGCGGAGAGACTGGATGTGGTAAAACAACCCAAGTTCCACAGTTTCTTTATGAAGTTGGCATTGGTTCAAGTGTGTCTCCTCTTGGAAGTGGTATCCTTGGTGTTACTCAACCCCGTCGCATTGCAGTCTATGCTACTGCTAGTCGTGTGGCATATGAGCTTGGTGTACGCGTGGGTGAGGAGGTTGGGTATCAAGTTAGATATGACAAAAAGATAGGCAAAAGTTGCTCCATCAACTTCATGACGGATGGAATATTGCTACGAGAACTCCAGGGTGATTTTTGGTTGAAGAGATACTCTGTTATAATTCTTGATGAGGTGCATGAGCGGAACTTGAGCACAGATACACTCATTGGAATGCTTTCACGTGTTATCATTTGTCGTCAGAAAGAGTATGAACGTCAGCAGGAGTTTTTGCAGCAGTACTCACATTTAGGAATATCTATAGATCGGGAGAAACTTGTTTTTCCACTGAAGCTTGTGCTTATGAGTGCTACCATGCCAGTGGAAGACTTCATTTCTTATAGGAAGCTGTTTCCTGACCCCCCACAAGAGATAAATGTTTTGTCTAGACAATTTAAAGTAGACATTCAGTTCTCAACGACAACTAAAGCTGATTATACTAAGGAAGCATGTAAAAAGGTCTTGGAAATTCACAAGAATATGCCACAAGGAGGCATACTTGTGTTTGTCACTGGGTACTGGGAAGTGGAGGTATTGTGTAAACAGCTACGTAGAGCTTCAAGGGAACTGACTATGAAAATAAAAAAAGGGTGTTGTGTGATGGAAGGTTCTGTAATAAACTCTGTTGGAGAGATAAATATGAAGGAGAACAGCGAAGCATTTCATTCAGCCAACCACCAGAATGATGTATTTAGTTACACTGATGAAGATCAGTGCGAGGTAGATGACGAGTTTGGTTCTTCATATGATACAGAAACAGAGAGTGAGCTGGAAATTATTGGTGACGATGGGGATTCATTGTGTCAAGATACCCCAGAAGTCTATGGTGAGGTTGCACAGATTTTAGGAGAGAACGTGAGCATTGCATCACTGAAAGCCTCTTTTGAATCATTGGCTGGAAAATCTTCTTCAGATTCTACTTGCAAAGAACCTATTTCACGAACTCTAGACTCTAGCTCTAGCCAATCCAATCATAGTCGCAGCTTGGGGAAAAAAGATGCCGTTGTAGATGATAGTTTTCCAGGCTCTCTGCGTGTTCTTCCTCTTTATTCTATGCTGCCTGCAGCAGCTCAGCATCGTGTATTTGATGAAGTAAAGGAAGGAGAACGGCTTGTTGTTGTGGCCACCAATGTTGCTGAAACCTCTGTCACTATACCTGGGATAAATTATGTTGTCGATACTGGAAAAGTGAAATCAAAGGAATACAACTTTAAAAATGGAAGTGAAATATATAAGGTACAGTGGATTAGTAAGGCATCGGCTGTTCAACGTGCAGGAAGAGCTGGAAGAACAGGGCCTGGTCACTGTTTCCGCCTTTATTCTTCGGCAGCATTTAATAATATCTTCGATGACAGTTCCCCATCTGAAATATCCAAAGTCCCTCTTGAAGGTCACATCCTTCTTTTGAAGTCCATGGACATTGAGTTGTCTGCTTTTCCTTTTCTTACTCGTCCAAATGATGATGCTCTTAATGGAGGACAGCGTTGCTTGGAGATCCTTGAAGCGCTTACTGAGGATGGAGAAGTGACACCTCTAGGGAAGGCCATGGCTTATTATCCCATAAGTCCTCGCCACTCTAAGATGCTCCTAACAGTCATCAAAATGTTGAATAAGGAGAAAAGTTATAAACGACCTAATTTAGTACTAGCATATGCAGTTGCTTCAGCTGCAGCTTTGAGTTTGTCAAATATTTTTGTTAGTCAGTTTGAAGACAGTTGCACAGAAAACCATGACTTAATTGATGGAAATTCCAGTGCTCCAGTTCACGGTGAAGTTATAGACAAGCAAGAGAGAGTAATCAAAGAGAAGAAAGTGAGAGCGTCTTGTGAAAATTTTAGTAGTCATAGCAGTGATGCCCTTTCCAGAGCTTATGTTTTGCGGCTCTATGAACTTTCCAAGAGCCGATTGCATTTCTGCAAGGACAATGCTCTACACCCAGAAACCATGCATGAAATATCCGAGCTGAGAGAGCAGCTACTCAAACTTGTCTTTTATCATAGTGGTGTGTCTGGCGGTGAATTTTCATGGATCCATGGTAGTCAGGAAGATGTTGAGCATGATTGGAGGGCTGATGTTATCCCTCTTTCTTTGGATGAGGAAGGCCTCTTGTGCCGGGCCATCTGTGCTGGTTGGGCAGATAGAGTTGCCAAGCGCATTAAAGGAAGTAAAAATCAATATCAAACCTGCTTGAGTAATGAGAATGTCTTCCTCGACCGCCAGTCATCTGTTTCTAAAATTGCTCCTGAATATTTGGTGTACAGTGAGTTGATACAGAAAAAGAAACCATACATGCACGGTGTTACTTGTATCGAACCAGAATGGCTTGTTGACTGTGGTCGGGTCTTATGCAAGCTTCCAAAGGGCAGTGCACCTTACTATGACTGTGTAAGTGACAAGGTACTATACAATGTCACTCCAACCTTTGGTCCTCATTTGTGGAAGCTTCCGGAACATTGTTTGCCTATTGATAAGACTGAGTTTAAAGGTGCAGTGCAAGCTTTTGCTTTTGCTTTGTTGGACGGCAAGGTTCTGCGTTGCCTGAGACCTGTGCGTGCATTCATGGCAGAACATCCTCGTACTGTTTTAAAGCCGGTGGCAGCAAATCGAGAACGGGTTCAGAATCTCTTGTTGAAGTTGGGGGAGAAGAAGATACACAGCCGTGCTATGTTGAGAGAAGTGTGGAGTAAAAATCCCAACGAATTGTATCTGGAAATCCGGAACTGGTTTAGAAAGAATTTCCACTATAAGTTTAAGGATATTTGGGTCGAAATGCTTCGTGAAGCCGTTTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1225

Amino Acids

138.32

Weight (kDa)

7.32

Isoelectric Point (pI)

47.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	220 - 402	2.9e-06	DEAD/DEAH box helicase
Helicase_C	PF00271	638 - 727	4.8e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	790 - 816	1.7e-06	Helicase associated domain (HA2), winged-helix
OB_NTP_bind	PF07717	998 - 1073	1.2e-17	Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C	PF23362	1142 - 1219	9.9e-16	DEAH helicase DHX37 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	647, 1007, 2511
Acc36I	ACCTGC	1 cut(s)	3077
AccB7I	CCANNNNNTGG	2 cut(s)	461, 3308
AccBSI	CCGCTC	1 cut(s)	1030
AccI	GTMKAC	2 cut(s)	383, 1287
AccII	CGCG	2 cut(s)	705, 873
AccIII	TCCGGA	2 cut(s)	3331, 3600
AciI	CCGC	7 cut(s)	592, 705, 1030, 2197, 2764, 2898, 3100
AclWI	GGATC	6 cut(s)	360, 486, 572, 2374, 2908, 2921
AcoI	YGGCCR	2 cut(s)	238, 2007
AcsI	RAATTY	6 cut(s)	685, 1353, 2632, 2716, 2903, 3619
AcuI	CTGAAG	4 cut(s)	633, 1196, 1779, 2532
AcvI	CACGTG	1 cut(s)	1070
AfaI	GTAC	9 cut(s)	369, 870, 1127, 1403, 2124, 2529, 3143, 3285, 3463
AfeI	AGCGCT	1 cut(s)	2391
AfiI	CCNNNNNNNGG	5 cut(s)	461, 1376, 2273, 2651, 3308
AflIII	ACRYGT	4 cut(s)	6, 378, 871, 1069
AhdI	GACNNNNNGTC	1 cut(s)	3103
AjiI	CACGTC	1 cut(s)	381
AjnI	CCWGG	5 cut(s)	240, 972, 1906, 2041, 2182
AjuI	GAANNNNNNNTTGG	2 cut(s)	747, 779
AleI	CACNNNNGTG	1 cut(s)	3543
Alw21I	GWGCWC	4 cut(s)	101, 1043, 2644, 3256
Alw26I	GTCTC	3 cut(s)	704, 783, 3421
Alw44I	GTGCAC	1 cut(s)	3252
AlwI	GGATC	6 cut(s)	360, 486, 572, 2374, 2908, 2921
AlwNI	CAGNNNCTG	2 cut(s)	203, 2183
Aor13HI	TCCGGA	2 cut(s)	3331, 3600
Aor51HI	AGCGCT	1 cut(s)	2391
AoxI	GGCC	8 cut(s)	238, 425, 672, 2007, 2180, 2427, 2986, 3000
ApaLI	GTGCAC	1 cut(s)	3252
ApoI	RAATTY	6 cut(s)	685, 1353, 2632, 2716, 2903, 3619
Asp700I	GAANNNNTTC	6 cut(s)	1063, 1545, 1789, 3089, 3498, 3657
AspLEI	GCGC	2 cut(s)	2392, 3039
AspS9I	GGNCC	3 cut(s)	2180, 3000, 3311
AsuC2I	CCSGG	1 cut(s)	2999
AsuHPI	GGTGA	6 cut(s)	890, 989, 1679, 1724, 2666, 2912
AvaII	GGWCC	1 cut(s)	3311
BaeGI	GKGCMC	1 cut(s)	3256
BalI	TGGCCA	2 cut(s)	240, 2009
BamHI	GGATCC	1 cut(s)	2913
BanII	GRGCYC	1 cut(s)	214
BbrPI	CACGTG	1 cut(s)	1070
BbsI	GAAGAC	3 cut(s)	1218, 2601, 3082
Bbv12I	GWGCWC	4 cut(s)	101, 1043, 2644, 3256
BceAI	ACGGC	5 cut(s)	1871, 2009, 3421, 3528, 3653
BciT130I	CCWGG	5 cut(s)	242, 974, 1908, 2043, 2184
BciVI	GTATCC	1 cut(s)	806
BcnI	CCSGG	1 cut(s)	2999
BcoDI	GTCTC	3 cut(s)	704, 783, 3421
BfaI	CTAG	7 cut(s)	843, 1272, 1832, 1839, 1845, 2420, 2531
BfmI	CTRYAG	3 cut(s)	1146, 1947, 2553
BfoI	RGCGCY	1 cut(s)	2393
BfuAI	ACCTGC	1 cut(s)	3077
BfuI	GTATCC	1 cut(s)	806
BlpI	GCTNAGC	1 cut(s)	1956
BmcAI	AGTACT	2 cut(s)	1127, 2529
Bme1390I	CCNGG	6 cut(s)	242, 974, 1908, 2043, 2184, 2999
Bme18I	GGWCC	1 cut(s)	3311
BmeRI	GACNNNNNGTC	1 cut(s)	3103
BmgBI	CACGTC	1 cut(s)	381
BmgT120I	GGNCC	3 cut(s)	2180, 3000, 3311
BmiI	GGNNCC	1 cut(s)	2915
BmrFI	CCNGG	6 cut(s)	242, 974, 1908, 2043, 2184, 2999
BmrI	ACTGGG	2 cut(s)	1407, 1414
BmsI	GCATC	7 cut(s)	1760, 1873, 1969, 2150, 2338, 2457, 2728
BmuI	ACTGGG	2 cut(s)	1407, 1414
BoxI	GACNNNNGTC	1 cut(s)	3219
BpiI	GAAGAC	3 cut(s)	1218, 2601, 3082
BplI	GAGNNNNNCTC	2 cut(s)	88, 120
BpmI	CTGGAG	3 cut(s)	508, 956, 2628
Bpu1102I	GCTNAGC	1 cut(s)	1956
BpuEI	CTTGAG	2 cut(s)	1057, 3094
BpuMI	CCSGG	1 cut(s)	2999
BsaAI	YACGTR	2 cut(s)	1070, 1436
BsaBI	GATNNNNATC	1 cut(s)	1593
BsaI	GGTCTC	1 cut(s)	3421
BsaJI	CCNNGG	6 cut(s)	799, 973, 2042, 2298, 2430, 2917
BsaWI	WCCGGW	3 cut(s)	219, 3331, 3600
Bsc4I	CCNNNNNNNGG	5 cut(s)	461, 1376, 2273, 2651, 3308
Bse118I	RCCGGY	1 cut(s)	3475
Bse3DI	GCAATG	2 cut(s)	822, 1746
Bse8I	GATNNNNATC	1 cut(s)	1593
BseAI	TCCGGA	2 cut(s)	3331, 3600
BseBI	CCWGG	5 cut(s)	242, 974, 1908, 2043, 2184
BseDI	CCNNGG	6 cut(s)	799, 973, 2042, 2298, 2430, 2917
BseGI	GGATG	8 cut(s)	96, 296, 722, 955, 2280, 2407, 2983, 3454
BseJI	GATNNNNATC	1 cut(s)	1593
BseLI	CCNNNNNNNGG	5 cut(s)	461, 1376, 2273, 2651, 3308
BseMI	GCAATG	2 cut(s)	822, 1746
BseMII	CTCAG	7 cut(s)	315, 522, 1970, 2388, 2840, 3351, 3416
BseRI	GAGGAG	4 cut(s)	149, 388, 771, 896
BseSI	GKGCMC	1 cut(s)	3256
BsgI	GTGCAG	5 cut(s)	189, 266, 543, 2178, 3393
Bsh1236I	CGCG	2 cut(s)	705, 873
Bsh1285I	CGRYCG	1 cut(s)	3100
BshFI	GGCC	8 cut(s)	240, 427, 674, 2009, 2182, 2429, 2988, 3002
BsiEI	CGRYCG	1 cut(s)	3100
BsiHKAI	GWGCWC	4 cut(s)	101, 1043, 2644, 3256
BsiSI	CCGG	5 cut(s)	220, 2998, 3332, 3476, 3601
BslFI	GGGAC	1 cut(s)	2249
BslI	CCNNNNNNNGG	5 cut(s)	461, 1376, 2273, 2651, 3308
BsmAI	GTCTC	3 cut(s)	704, 783, 3421
BsmFI	GGGAC	1 cut(s)	2249
BsmI	GAATGC	2 cut(s)	1065, 3440
BsnI	GGCC	8 cut(s)	240, 427, 674, 2009, 2182, 2429, 2988, 3002
Bso31I	GGTCTC	1 cut(s)	3421
Bsp1286I	GDGCHC	5 cut(s)	101, 214, 1043, 2644, 3256
Bsp13I	TCCGGA	2 cut(s)	3331, 3600
Bsp1407I	TGTACA	1 cut(s)	3141
Bsp143I	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
Bsp1720I	GCTNAGC	1 cut(s)	1956
Bsp19I	CCATGG	3 cut(s)	2298, 2430, 2917
BspACI	CCGC	7 cut(s)	592, 705, 1030, 2197, 2764, 2898, 3100
BspANI	GGCC	8 cut(s)	240, 427, 674, 2009, 2182, 2429, 2988, 3002
BspCNI	CTCAG	7 cut(s)	316, 523, 1969, 2389, 2841, 3352, 3417
BspEI	TCCGGA	2 cut(s)	3331, 3600
BspFNI	CGCG	2 cut(s)	705, 873
BspHI	TCATGA	1 cut(s)	942
BspLI	GGNNCC	1 cut(s)	2915
BspMAI	CTGCAG	2 cut(s)	1951, 2557
BspMI	ACCTGC	1 cut(s)	3077
BspPI	GGATC	6 cut(s)	360, 486, 572, 2374, 2908, 2921
BspQI	GCTCTTC	2 cut(s)	219, 2158
BspTNI	GGTCTC	1 cut(s)	3421
BsrBI	CCGCTC	1 cut(s)	1030
BsrDI	GCAATG	2 cut(s)	822, 1746
BsrFI	RCCGGY	1 cut(s)	3475
BsrGI	TGTACA	1 cut(s)	3141
BssAI	RCCGGY	1 cut(s)	3475
BssECI	CCNNGG	6 cut(s)	799, 973, 2042, 2298, 2430, 2917
BssMI	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
BssT1I	CCWWGG	4 cut(s)	799, 2298, 2430, 2917
Bst2UI	CCWGG	5 cut(s)	242, 974, 1908, 2043, 2184
Bst6I	CTCTTC	4 cut(s)	219, 516, 986, 2158
BstAPI	GCANNNNNTGC	2 cut(s)	833, 927
BstAUI	TGTACA	1 cut(s)	3141
BstBAI	YACGTR	2 cut(s)	1070, 1436
BstC8I	GCNNGC	4 cut(s)	1947, 3236, 3381, 3438
BstDSI	CCRYGG	3 cut(s)	2298, 2430, 2917
BstENI	CCTNNNNNAGG	1 cut(s)	2271
BstF5I	GGATG	8 cut(s)	96, 296, 722, 955, 2280, 2407, 2983, 3454
BstFNI	CGCG	2 cut(s)	705, 873
BstH2I	RGCGCY	1 cut(s)	2393
BstHHI	GCGC	2 cut(s)	2392, 3039
BstKTI	GATC	7 cut(s)	355, 481, 580, 1153, 1597, 2382, 2916
BstMAI	GTCTC	3 cut(s)	704, 783, 3421
BstMBI	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
BstMCI	CGRYCG	1 cut(s)	3100
BstNI	CCWGG	5 cut(s)	242, 974, 1908, 2043, 2184
BstNSI	RCATGY	4 cut(s)	10, 599, 1338, 3178
BstPAI	GACNNNNGTC	1 cut(s)	3219
BstSCI	CCNGG	6 cut(s)	240, 972, 1906, 2041, 2182, 2997
BstSFI	CTRYAG	3 cut(s)	1146, 1947, 2553
BstSLI	GKGCMC	1 cut(s)	3256
BstSNI	TACGTA	1 cut(s)	1436
BstUI	CGCG	2 cut(s)	705, 873
BstV2I	GAAGAC	3 cut(s)	1218, 2601, 3082
BstX2I	RGATCY	3 cut(s)	577, 2379, 2913
BstXI	CCANNNNNNTGG	1 cut(s)	1207
BstYI	RGATCY	3 cut(s)	577, 2379, 2913
BsuI	GTATCC	1 cut(s)	806
BsuRI	GGCC	8 cut(s)	240, 427, 674, 2009, 2182, 2429, 2988, 3002
BtgI	CCRYGG	3 cut(s)	2298, 2430, 2917
BtrI	CACGTC	1 cut(s)	381
BtsCI	GGATG	8 cut(s)	96, 296, 722, 955, 2280, 2407, 2983, 3454
BtsI	GCAGTG	3 cut(s)	2740, 3256, 3381
BveI	ACCTGC	1 cut(s)	3077
Cac8I	GCNNGC	4 cut(s)	1947, 3236, 3381, 3438
CaiI	CAGNNNCTG	2 cut(s)	203, 2183
CciI	TCATGA	1 cut(s)	942
CfoI	GCGC	2 cut(s)	2392, 3039
Cfr10I	RCCGGY	1 cut(s)	3475
Cfr13I	GGNCC	3 cut(s)	2180, 3000, 3311
CseI	GACGC	1 cut(s)	2694
Csp6I	GTAC	9 cut(s)	368, 869, 1126, 1402, 2123, 2528, 3142, 3284, 3462
CspCI	CAANNNNNGTGG	2 cut(s)	1999, 2034
CviQI	GTAC	9 cut(s)	368, 869, 1126, 1402, 2123, 2528, 3142, 3284, 3462
DpnI	GATC	7 cut(s)	354, 480, 579, 1152, 1596, 2381, 2915
DpnII	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
DraI	TTTAAA	4 cut(s)	1282, 2098, 3367, 3471
DriI	GACNNNNNGTC	1 cut(s)	3103
EaeI	YGGCCR	2 cut(s)	238, 2007
Eam1104I	CTCTTC	4 cut(s)	219, 516, 986, 2158
Eam1105I	GACNNNNNGTC	1 cut(s)	3103
EarI	CTCTTC	4 cut(s)	219, 516, 986, 2158
EciI	GGCGGA	1 cut(s)	2186
Eco105I	TACGTA	1 cut(s)	1436
Eco130I	CCWWGG	4 cut(s)	799, 2298, 2430, 2917
Eco147I	AGGCCT	2 cut(s)	427, 2988
Eco24I	GRGCYC	1 cut(s)	214
Eco31I	GGTCTC	1 cut(s)	3421
Eco47I	GGWCC	1 cut(s)	3311
Eco47III	AGCGCT	1 cut(s)	2391
Eco57I	CTGAAG	4 cut(s)	633, 1196, 1779, 2532
Eco72I	CACGTG	1 cut(s)	1070
EcoNI	CCTNNNNNAGG	1 cut(s)	2271
EcoO109I	RGGNCCY	1 cut(s)	2180
EcoRII	CCWGG	5 cut(s)	240, 972, 1906, 2041, 2182
EcoT14I	CCWWGG	4 cut(s)	799, 2298, 2430, 2917
EcoT22I	ATGCAT	1 cut(s)	2835
EcoT38I	GRGCYC	1 cut(s)	214
ErhI	CCWWGG	4 cut(s)	799, 2298, 2430, 2917
FalI	AAGNNNNNCTT	2 cut(s)	2253, 2285
FaqI	GGGAC	1 cut(s)	2249
FauNDI	CATATG	3 cut(s)	856, 1630, 2536
FblI	GTMKAC	2 cut(s)	383, 1287
FokI	GGATG	8 cut(s)	103, 283, 729, 962, 2267, 2414, 2990, 3441
FriOI	GRGCYC	1 cut(s)	214
FspBI	CTAG	7 cut(s)	843, 1272, 1832, 1839, 1845, 2420, 2531
GlaI	GCGC	2 cut(s)	2391, 3038
GsuI	CTGGAG	3 cut(s)	508, 956, 2628
HaeII	RGCGCY	1 cut(s)	2393
HaeIII	GGCC	8 cut(s)	240, 427, 674, 2009, 2182, 2429, 2988, 3002
HapII	CCGG	5 cut(s)	220, 2998, 3332, 3476, 3601
HgaI	GACGC	1 cut(s)	2694
HhaI	GCGC	2 cut(s)	2392, 3039
Hin6I	GCGC	2 cut(s)	2390, 3037
HinP1I	GCGC	2 cut(s)	2390, 3037
HincII	GTYRAC	1 cut(s)	3214
HindII	GTYRAC	1 cut(s)	3214
HindIII	AAGCTT	5 cut(s)	551, 1178, 3236, 3326, 3381
HpaII	CCGG	5 cut(s)	220, 2998, 3332, 3476, 3601
HphI	GGTGA	6 cut(s)	890, 989, 1679, 1724, 2666, 2912
Hpy188I	TCNGA	9 cut(s)	331, 394, 573, 582, 1089, 1798, 2251, 2845, 3504
Hpy99I	CGWCG	1 cut(s)	822
HpyCH4IV	ACGT	6 cut(s)	380, 1069, 1102, 1435, 1740, 2155
HpySE526I	ACGT	6 cut(s)	380, 1069, 1102, 1435, 1740, 2155
HspAI	GCGC	2 cut(s)	2390, 3037
Kpn2I	TCCGGA	2 cut(s)	3331, 3600
Kzo9I	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
LguI	GCTCTTC	2 cut(s)	219, 2158
LmnI	GCTCC	5 cut(s)	655, 935, 2475, 2647, 3130
LweI	GCATC	7 cut(s)	1760, 1873, 1969, 2150, 2338, 2457, 2728
MaeI	CTAG	7 cut(s)	843, 1272, 1832, 1839, 1845, 2420, 2531
MaeII	ACGT	6 cut(s)	380, 1069, 1102, 1435, 1740, 2155
MalI	GATC	7 cut(s)	354, 480, 579, 1152, 1596, 2381, 2915
MbiI	CCGCTC	1 cut(s)	1030
MboI	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
MflI	RGATCY	3 cut(s)	577, 2379, 2913
MhlI	GDGCHC	5 cut(s)	101, 214, 1043, 2644, 3256
MlsI	TGGCCA	2 cut(s)	240, 2009
MluI	ACGCGT	1 cut(s)	871
MluNI	TGGCCA	2 cut(s)	240, 2009
MlyI	GAGTC	5 cut(s)	335, 389, 535, 542, 1829
MmeI	TCCRAC	4 cut(s)	309, 1492, 3326, 3381
Mox20I	TGGCCA	2 cut(s)	240, 2009
Mph1103I	ATGCAT	1 cut(s)	2835
MroI	TCCGGA	2 cut(s)	3331, 3600
MroXI	GAANNNNTTC	6 cut(s)	1063, 1545, 1789, 3089, 3498, 3657
MscI	TGGCCA	2 cut(s)	240, 2009
MslI	CAYNNNNRTG	8 cut(s)	1058, 1205, 1385, 1566, 2486, 2733, 2886, 3543
Msp20I	TGGCCA	2 cut(s)	240, 2009
MspA1I	CMGCKG	1 cut(s)	2552
MspI	CCGG	5 cut(s)	220, 2998, 3332, 3476, 3601
MspR9I	CCNGG	6 cut(s)	242, 974, 1908, 2043, 2184, 2999
Mva1269I	GAATGC	2 cut(s)	1065, 3440
MvaI	CCWGG	5 cut(s)	242, 974, 1908, 2043, 2184
MvnI	CGCG	2 cut(s)	705, 873
NciI	CCSGG	1 cut(s)	2999
NcoI	CCATGG	3 cut(s)	2298, 2430, 2917
NdeI	CATATG	3 cut(s)	856, 1630, 2536
NdeII	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
NlaIV	GGNNCC	1 cut(s)	2915
NmuCI	GTSAC	9 cut(s)	62, 1393, 1667, 2032, 2186, 2276, 2410, 3275, 3295
NsiI	ATGCAT	1 cut(s)	2835
NspI	RCATGY	4 cut(s)	10, 599, 1338, 3178
OliI	CACNNNNGTG	1 cut(s)	3543
PagI	TCATGA	1 cut(s)	942
PceI	AGGCCT	2 cut(s)	427, 2988
PciI	ACATGT	1 cut(s)	6
PciSI	GCTCTTC	2 cut(s)	219, 2158
PctI	GAATGC	2 cut(s)	1065, 3440
PdmI	GAANNNNTTC	6 cut(s)	1063, 1545, 1789, 3089, 3498, 3657
PfeI	GAWTC	6 cut(s)	193, 248, 1679, 1772, 1799, 3505
PflMI	CCANNNNNTGG	2 cut(s)	461, 3308
PleI	GAGTC	5 cut(s)	334, 389, 534, 541, 1829
PmaCI	CACGTG	1 cut(s)	1070
PmlI	CACGTG	1 cut(s)	1070
PpsI	GAGTC	5 cut(s)	334, 389, 534, 541, 1829
Ppu21I	YACGTR	2 cut(s)	1070, 1436
PscI	ACATGT	1 cut(s)	6
PshAI	GACNNNNGTC	1 cut(s)	3219
PsiI	TTATAA	3 cut(s)	647, 1007, 2511
Psp6I	CCWGG	5 cut(s)	240, 972, 1906, 2041, 2182
PspCI	CACGTG	1 cut(s)	1070
PspGI	CCWGG	5 cut(s)	240, 972, 1906, 2041, 2182
PspN4I	GGNNCC	1 cut(s)	2915
PspPI	GGNCC	3 cut(s)	2180, 3000, 3311
PstI	CTGCAG	2 cut(s)	1951, 2557
PstNI	CAGNNNCTG	2 cut(s)	203, 2183
PsuI	RGATCY	3 cut(s)	577, 2379, 2913
PvuII	CAGCTG	1 cut(s)	2552
RsaI	GTAC	9 cut(s)	369, 870, 1127, 1403, 2124, 2529, 3143, 3285, 3463
RsaNI	GTAC	9 cut(s)	368, 869, 1126, 1402, 2123, 2528, 3142, 3284, 3462
RseI	CAYNNNNRTG	8 cut(s)	1058, 1205, 1385, 1566, 2486, 2733, 2886, 3543
SapI	GCTCTTC	2 cut(s)	219, 2158
Sau3AI	GATC	7 cut(s)	352, 478, 577, 1150, 1594, 2379, 2913
Sau96I	GGNCC	3 cut(s)	2180, 3000, 3311
ScaI	AGTACT	2 cut(s)	1127, 2529
SchI	GAGTC	5 cut(s)	335, 389, 535, 542, 1829
ScrFI	CCNGG	6 cut(s)	242, 974, 1908, 2043, 2184, 2999
SduI	GDGCHC	5 cut(s)	101, 214, 1043, 2644, 3256
SfaNI	GCATC	7 cut(s)	1760, 1873, 1969, 2150, 2338, 2457, 2728
SfcI	CTRYAG	3 cut(s)	1146, 1947, 2553
SinI	GGWCC	1 cut(s)	3311
SmiMI	CAYNNNNRTG	8 cut(s)	1058, 1205, 1385, 1566, 2486, 2733, 2886, 3543
SmlI	CTYRAG	2 cut(s)	1036, 3073
SmoI	CTYRAG	2 cut(s)	1036, 3073
SnaBI	TACGTA	1 cut(s)	1436
SseBI	AGGCCT	2 cut(s)	427, 2988
SsiI	CCGC	7 cut(s)	592, 705, 1030, 2197, 2764, 2898, 3100
SspI	AATATT	4 cut(s)	235, 957, 2575, 3134
SspMI	CTAG	7 cut(s)	843, 1272, 1832, 1839, 1845, 2420, 2531
StuI	AGGCCT	2 cut(s)	427, 2988
StyD4I	CCNGG	6 cut(s)	240, 972, 1906, 2041, 2182, 2997
StyI	CCWWGG	4 cut(s)	799, 2298, 2430, 2917
TaiI	ACGT	6 cut(s)	383, 1072, 1105, 1438, 1743, 2158
TaqI	TCGA	6 cut(s)	2061, 2232, 3096, 3195, 3490, 3651
TatI	WGTACW	3 cut(s)	1125, 2527, 3141
TauI	GCSGC	2 cut(s)	595, 2767
TfiI	GAWTC	6 cut(s)	193, 248, 1679, 1772, 1799, 3505
TseFI	GTSAC	9 cut(s)	62, 1393, 1667, 2032, 2186, 2276, 2410, 3275, 3295
Tsp45I	GTSAC	9 cut(s)	62, 1393, 1667, 2032, 2186, 2276, 2410, 3275, 3295
TspGWI	ACGGA	1 cut(s)	962
Van91I	CCANNNNNTGG	2 cut(s)	461, 3308
VneI	GTGCAC	1 cut(s)	3252
VpaK11BI	GGWCC	1 cut(s)	3311
XagI	CCTNNNNNAGG	1 cut(s)	2271
XapI	RAATTY	6 cut(s)	685, 1353, 2632, 2716, 2903, 3619
XbaI	TCTAGA	2 cut(s)	1271, 1831
XceI	RCATGY	4 cut(s)	10, 599, 1338, 3178
XmiI	GTMKAC	2 cut(s)	383, 1287
XmnI	GAANNNNTTC	6 cut(s)	1063, 1545, 1789, 3089, 3498, 3657
XspI	CTAG	7 cut(s)	843, 1272, 1832, 1839, 1845, 2420, 2531
ZrmI	AGTACT	2 cut(s)	1127, 2529
Zsp2I	ATGCAT	1 cut(s)	2835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.