Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Forward (+)

38982392 .. 38984650

Gene Structure 2259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ19707

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGCTGCCTGCAGCAGCTCAGCATCGTGTATTTGATGAAGTAAAGGAGGGAGAACGGCTTGTTGTTGTGGCCACCAATGTTGCTGAAACCTCTGTCACTATACCTGGGATAAAGTATGTTGTCGATACTGGAAAAGTGAAATCAAAGGAATACAACTTTAAAAATGGAAGTGAAATATATAAAGTACAGTGGATTAGTAAGGCATCGGCTGTTCAACGTGCAGGAAGAGCTGGAAGAACAGGGCCTGGTCACTGTTTCCGCCTTTATTCTTCGGCAGCATTTAATAATATCTTCGATGACAGTTCCCCATCTGAAATATCCAAAGTCCCTCTTGAAGGTCACATCCTTCTTTTGAAGTCCATGGACATTGAGTTGTCTGCTTTTCCTTTTCTTACTCGTCCAAATGATGATGCTCTTAATGGAGGACAGCGTTGCTTGGAGATCCTTGAAGCGCTTACTGAGGATGGAGAAGTGACACCTCTAGGGAAGGCCATGGCTTATTATCCCATAAGTCCTCGCCACTCTAAGATGCTCCTAACAGTCATCAAAATGTTGAATAAGGAGAAAAGTTATAAACGACCTAATTTAGTACTAGCATATGCAGTTGCTTCAGCTGCAGCTTTGAGTTTGTCAAATATTTTTGTTAGTCAGTTTGAAGACAGTTGCACAGAAAACCATGACTTAATTGATGGAAATTCCAGTGCTCCAGTTCACGGTGAAGTTATAGACAAGCAAGACAGAGTGATCAAAGAGAAGAAAGTGAGAGCGTCTTGTGAAAATTTTAGTAGTCATAGCAGTGATGCCCTTTCCAGAGCTTATGTTTTGCGGCTCTATGAACTTTCCAAGAGCCGATTGCATTTCTGCAAGGACAATGCTCTACACCCAGAAACCATGCATGAAATATCCGAGCTGAGAGAGCAGCTACTCAAACTTGTCTTTTATCATAGTGGTGTGTCTGGCGGTGAATTTTCATGGATCCATGGTAGTCAGGAAGATGTTGAGCATGATTGGAGGGCTGATGTTATCCCTCTTTCTTTGGATGAGGAAGGCCTCTTGTGCCGGGCCATCTGTGCTGGTTGGGCAGATAGAGTTGCCAAGCGCATTAAAGGAAGTAAAAATCAATATCAAACCTGCTTGAGTAATGAGAATGTCTTTCTCGACCGCCAGTCATCTGTTTCTAAAATTGCTCCTGAATATTTGGTGTACAGTGAGTTGATACAGAAAAAGAAACCATACATGCACGGTGTTACTTGTATCGAACCAGAATGGCTTGTTGACTGTGGTCGGGTCTTATGCAAGCTTCCAAAGGGCAGTGCACCTTACTATGACTGTGTAAGTGACAAGGTACTATACAATGTCACTCCAACCTTTGGTCCTCATTTGTGGAAGCTTCCGGAACATTGTTTGCCTATTGATAAGACTGAGTTTAAAGGTGCAGTGCAAGCTTTTGCTTTTGCTTTGTTGGACGGCAAGGTTCTGCGTTGCCTGAGACCTGTGCGTGCATTCATGGCAGAACATCCTCGTACTGTTTTAAAGCCAGTGGCAGCAAATCGAGAACGGGTTCAGAATCTCTTGTTGAAGTTGGGGGAGAAGAAGATACACAGCCGTGCTATGTTGAGAGCAGTGTGGAGTAAAAATCCCAACGAATTGTATCTGGAAATCCGGAACTGGTTTAGAAAGAATTTCCACTATAAGTTTAAGGATATTTGGGTCGAAATGCTTCGTGAAGCCGTTTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

579

Amino Acids

65.52

Weight (kDa)

8.64

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	3 - 81	5e-09	Helicase conserved C-terminal domain
WHD_HA2	PF04408	144 - 170	7.3e-07	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	171 - 269	7.1e-06	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	352 - 427	4.3e-18	Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C	PF23362	496 - 573	6.4e-16	DEAH helicase DHX37 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	573
Acc36I	ACCTGC	1 cut(s)	1139
AccB7I	CCANNNNNTGG	1 cut(s)	1370
AccIII	TCCGGA	2 cut(s)	1393, 1662
AciI	CCGC	4 cut(s)	259, 826, 960, 1162
AclWI	GGATC	3 cut(s)	436, 970, 983
AcoI	YGGCCR	1 cut(s)	69
AcsI	RAATTY	4 cut(s)	694, 778, 965, 1681
AcuI	CTGAAG	1 cut(s)	594
AfaI	GTAC	5 cut(s)	186, 591, 1205, 1347, 1525
AfeI	AGCGCT	1 cut(s)	453
AfiI	CCNNNNNNNGG	3 cut(s)	335, 713, 1370
AgsI	TTSAA	7 cut(s)	215, 335, 355, 449, 556, 656, 1579
AhdI	GACNNNNNGTC	1 cut(s)	1165
AjnI	CCWGG	2 cut(s)	103, 244
AleI	CACNNNNGTG	1 cut(s)	1605
Alw21I	GWGCWC	2 cut(s)	706, 1318
Alw26I	GTCTC	1 cut(s)	1483
Alw44I	GTGCAC	1 cut(s)	1314
AlwI	GGATC	3 cut(s)	436, 970, 983
AlwNI	CAGNNNCTG	1 cut(s)	245
Aor13HI	TCCGGA	2 cut(s)	1393, 1662
Aor51HI	AGCGCT	1 cut(s)	453
AoxI	GGCC	5 cut(s)	69, 242, 489, 1048, 1062
ApaLI	GTGCAC	1 cut(s)	1314
ApeKI	GCWGC	8 cut(s)	4, 11, 14, 275, 614, 617, 919, 1544
ApoI	RAATTY	4 cut(s)	694, 778, 965, 1681
Asp700I	GAANNNNTTC	2 cut(s)	1560, 1719
AspLEI	GCGC	2 cut(s)	454, 1101
AspS9I	GGNCC	3 cut(s)	242, 1062, 1373
AsuC2I	CCSGG	1 cut(s)	1061
AsuHPI	GGTGA	2 cut(s)	728, 974
AvaII	GGWCC	1 cut(s)	1373
BaeGI	GKGCMC	1 cut(s)	1318
BalI	TGGCCA	1 cut(s)	71
BamHI	GGATCC	1 cut(s)	975
BbsI	GAAGAC	1 cut(s)	663
Bbv12I	GWGCWC	2 cut(s)	706, 1318
BbvI	GCAGC	7 cut(s)	23, 26, 287, 601, 629, 931, 1556
BccI	CCATC	4 cut(s)	316, 458, 683, 1073
BceAI	ACGGC	4 cut(s)	71, 1483, 1590, 1715
BciT130I	CCWGG	2 cut(s)	105, 246
BclI	TGATCA	1 cut(s)	744
BcnI	CCSGG	1 cut(s)	1061
BcoDI	GTCTC	1 cut(s)	1483
BfaI	CTAG	2 cut(s)	482, 593
BfmI	CTRYAG	2 cut(s)	9, 615
BfoI	RGCGCY	1 cut(s)	455
BfuAI	ACCTGC	1 cut(s)	1139
BisI	GCNGC	9 cut(s)	5, 12, 15, 276, 615, 618, 827, 920, 1545
BlpI	GCTNAGC	1 cut(s)	18
BlsI	GCNGC	9 cut(s)	6, 13, 16, 277, 616, 619, 828, 921, 1546
BmcAI	AGTACT	1 cut(s)	591
Bme1390I	CCNGG	3 cut(s)	105, 246, 1061
Bme18I	GGWCC	1 cut(s)	1373
BmeRI	GACNNNNNGTC	1 cut(s)	1165
BmgT120I	GGNCC	3 cut(s)	242, 1062, 1373
BmiI	GGNNCC	1 cut(s)	977
BmrFI	CCNGG	3 cut(s)	105, 246, 1061
BmsI	GCATC	5 cut(s)	31, 212, 400, 519, 790
BoxI	GACNNNNGTC	1 cut(s)	1281
BpiI	GAAGAC	1 cut(s)	663
BpmI	CTGGAG	1 cut(s)	690
Bpu1102I	GCTNAGC	1 cut(s)	18
BpuEI	CTTGAG	1 cut(s)	1156
BpuMI	CCSGG	1 cut(s)	1061
BsaI	GGTCTC	1 cut(s)	1483
BsaJI	CCNNGG	4 cut(s)	104, 360, 492, 979
BsaWI	WCCGGW	2 cut(s)	1393, 1662
Bsc4I	CCNNNNNNNGG	3 cut(s)	335, 713, 1370
Bse1I	ACTGG	6 cut(s)	133, 699, 707, 1165, 1538, 1673
BseAI	TCCGGA	2 cut(s)	1393, 1662
BseBI	CCWGG	2 cut(s)	105, 246
BseDI	CCNNGG	4 cut(s)	104, 360, 492, 979
BseGI	GGATG	4 cut(s)	342, 469, 1045, 1516
BseLI	CCNNNNNNNGG	3 cut(s)	335, 713, 1370
BseMII	CTCAG	5 cut(s)	32, 450, 902, 1413, 1478
BseNI	ACTGG	6 cut(s)	133, 699, 707, 1165, 1538, 1673
BseSI	GKGCMC	1 cut(s)	1318
BseXI	GCAGC	7 cut(s)	23, 26, 287, 601, 629, 931, 1556
BsgI	GTGCAG	2 cut(s)	240, 1455
Bsh1285I	CGRYCG	1 cut(s)	1162
BshFI	GGCC	5 cut(s)	71, 244, 491, 1050, 1064
BsiEI	CGRYCG	1 cut(s)	1162
BsiHKAI	GWGCWC	2 cut(s)	706, 1318
BsiSI	CCGG	3 cut(s)	1060, 1394, 1663
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	3 cut(s)	335, 713, 1370
BsmAI	GTCTC	1 cut(s)	1483
BsmFI	GGGAC	1 cut(s)	311
BsmI	GAATGC	1 cut(s)	1502
BsnI	GGCC	5 cut(s)	71, 244, 491, 1050, 1064
Bso31I	GGTCTC	1 cut(s)	1483
Bsp1286I	GDGCHC	2 cut(s)	706, 1318
Bsp13I	TCCGGA	2 cut(s)	1393, 1662
Bsp1407I	TGTACA	1 cut(s)	1203
Bsp143I	GATC	3 cut(s)	441, 744, 975
Bsp1720I	GCTNAGC	1 cut(s)	18
Bsp19I	CCATGG	3 cut(s)	360, 492, 979
BspACI	CCGC	4 cut(s)	259, 826, 960, 1162
BspANI	GGCC	5 cut(s)	71, 244, 491, 1050, 1064
BspCNI	CTCAG	5 cut(s)	31, 451, 903, 1414, 1479
BspEI	TCCGGA	2 cut(s)	1393, 1662
BspLI	GGNNCC	1 cut(s)	977
BspMAI	CTGCAG	2 cut(s)	13, 619
BspMI	ACCTGC	1 cut(s)	1139
BspPI	GGATC	3 cut(s)	436, 970, 983
BspQI	GCTCTTC	1 cut(s)	220
BspTNI	GGTCTC	1 cut(s)	1483
BsrGI	TGTACA	1 cut(s)	1203
BsrI	ACTGG	6 cut(s)	133, 699, 707, 1165, 1538, 1673
BssECI	CCNNGG	4 cut(s)	104, 360, 492, 979
BssMI	GATC	3 cut(s)	441, 744, 975
BssT1I	CCWWGG	3 cut(s)	360, 492, 979
Bst2UI	CCWGG	2 cut(s)	105, 246
Bst6I	CTCTTC	1 cut(s)	220
BstAUI	TGTACA	1 cut(s)	1203
BstC8I	GCNNGC	4 cut(s)	9, 1298, 1443, 1500
BstDEI	CTNAG	6 cut(s)	18, 459, 525, 911, 1422, 1487
BstDSI	CCRYGG	3 cut(s)	360, 492, 979
BstENI	CCTNNNNNAGG	1 cut(s)	333
BstF5I	GGATG	4 cut(s)	342, 469, 1045, 1516
BstH2I	RGCGCY	1 cut(s)	455
BstHHI	GCGC	2 cut(s)	454, 1101
BstKTI	GATC	3 cut(s)	444, 747, 978
BstMAI	GTCTC	1 cut(s)	1483
BstMBI	GATC	3 cut(s)	441, 744, 975
BstMCI	CGRYCG	1 cut(s)	1162
BstMWI	GCNNNNNNNGC	8 cut(s)	227, 614, 916, 1056, 1070, 1079, 1442, 1508
BstNI	CCWGG	2 cut(s)	105, 246
BstNSI	RCATGY	1 cut(s)	1240
BstPAI	GACNNNNGTC	1 cut(s)	1281
BstSCI	CCNGG	3 cut(s)	103, 244, 1059
BstSFI	CTRYAG	2 cut(s)	9, 615
BstSLI	GKGCMC	1 cut(s)	1318
BstV1I	GCAGC	7 cut(s)	23, 26, 287, 601, 629, 931, 1556
BstV2I	GAAGAC	1 cut(s)	663
BstX2I	RGATCY	2 cut(s)	441, 975
BstYI	RGATCY	2 cut(s)	441, 975
BsuRI	GGCC	5 cut(s)	71, 244, 491, 1050, 1064
BtgI	CCRYGG	3 cut(s)	360, 492, 979
BtsCI	GGATG	4 cut(s)	342, 469, 1045, 1516
BtsI	GCAGTG	4 cut(s)	802, 1318, 1443, 1629
BtsIMutI	CAGTG	9 cut(s)	194, 250, 706, 802, 1213, 1318, 1443, 1545, 1629
BveI	ACCTGC	1 cut(s)	1139
Cac8I	GCNNGC	4 cut(s)	9, 1298, 1443, 1500
CaiI	CAGNNNCTG	1 cut(s)	245
CfoI	GCGC	2 cut(s)	454, 1101
Cfr13I	GGNCC	3 cut(s)	242, 1062, 1373
CseI	GACGC	1 cut(s)	756
Csp6I	GTAC	5 cut(s)	185, 590, 1204, 1346, 1524
CspCI	CAANNNNNGTGG	2 cut(s)	61, 96
CviQI	GTAC	5 cut(s)	185, 590, 1204, 1346, 1524
DdeI	CTNAG	6 cut(s)	18, 459, 525, 911, 1422, 1487
DpnI	GATC	3 cut(s)	443, 746, 977
DpnII	GATC	3 cut(s)	441, 744, 975
DraI	TTTAAA	3 cut(s)	160, 1429, 1533
DriI	GACNNNNNGTC	1 cut(s)	1165
EaeI	YGGCCR	1 cut(s)	69
Eam1104I	CTCTTC	1 cut(s)	220
Eam1105I	GACNNNNNGTC	1 cut(s)	1165
EarI	CTCTTC	1 cut(s)	220
EciI	GGCGGA	1 cut(s)	248
Eco130I	CCWWGG	3 cut(s)	360, 492, 979
Eco147I	AGGCCT	1 cut(s)	1050
Eco31I	GGTCTC	1 cut(s)	1483
Eco47I	GGWCC	1 cut(s)	1373
Eco47III	AGCGCT	1 cut(s)	453
Eco57I	CTGAAG	1 cut(s)	594
EcoNI	CCTNNNNNAGG	1 cut(s)	333
EcoO109I	RGGNCCY	1 cut(s)	242
EcoRII	CCWGG	2 cut(s)	103, 244
EcoT14I	CCWWGG	3 cut(s)	360, 492, 979
EcoT22I	ATGCAT	1 cut(s)	897
ErhI	CCWWGG	3 cut(s)	360, 492, 979
FalI	AAGNNNNNCTT	2 cut(s)	315, 347
FaqI	GGGAC	1 cut(s)	311
FauNDI	CATATG	1 cut(s)	598
FbaI	TGATCA	1 cut(s)	744
Fnu4HI	GCNGC	9 cut(s)	5, 12, 15, 276, 615, 618, 827, 920, 1545
FokI	GGATG	4 cut(s)	329, 476, 1052, 1503
Fsp4HI	GCNGC	9 cut(s)	5, 12, 15, 276, 615, 618, 827, 920, 1545
FspBI	CTAG	2 cut(s)	482, 593
GlaI	GCGC	2 cut(s)	453, 1100
GluI	GCNGC	9 cut(s)	5, 12, 15, 276, 615, 618, 827, 920, 1545
GsuI	CTGGAG	1 cut(s)	690
HaeII	RGCGCY	1 cut(s)	455
HaeIII	GGCC	5 cut(s)	71, 244, 491, 1050, 1064
HapII	CCGG	3 cut(s)	1060, 1394, 1663
HgaI	GACGC	1 cut(s)	756
HhaI	GCGC	2 cut(s)	454, 1101
Hin6I	GCGC	2 cut(s)	452, 1099
HinP1I	GCGC	2 cut(s)	452, 1099
HincII	GTYRAC	1 cut(s)	1276
HindII	GTYRAC	1 cut(s)	1276
HindIII	AAGCTT	3 cut(s)	1298, 1388, 1443
HinfI	GANTC	1 cut(s)	1567
HpaII	CCGG	3 cut(s)	1060, 1394, 1663
HphI	GGTGA	2 cut(s)	728, 974
Hpy166II	GTNNAC	5 cut(s)	712, 1204, 1276, 1316, 1735
Hpy188I	TCNGA	3 cut(s)	313, 907, 1566
Hpy8I	GTNNAC	5 cut(s)	712, 1204, 1276, 1316, 1735
HpyAV	CCTTC	4 cut(s)	329, 356, 481, 1040
HpyCH4IV	ACGT	1 cut(s)	217
HpyF10VI	GCNNNNNNNGC	8 cut(s)	227, 614, 916, 1056, 1070, 1079, 1442, 1508
HpyF3I	CTNAG	6 cut(s)	18, 459, 525, 911, 1422, 1487
HpySE526I	ACGT	1 cut(s)	217
HspAI	GCGC	2 cut(s)	452, 1099
Kpn2I	TCCGGA	2 cut(s)	1393, 1662
Ksp22I	TGATCA	1 cut(s)	744
Kzo9I	GATC	3 cut(s)	441, 744, 975
LguI	GCTCTTC	1 cut(s)	220
LmnI	GCTCC	3 cut(s)	537, 709, 1192
Lsp1109I	GCAGC	7 cut(s)	23, 26, 287, 601, 629, 931, 1556
LweI	GCATC	5 cut(s)	31, 212, 400, 519, 790
MaeI	CTAG	2 cut(s)	482, 593
MaeII	ACGT	1 cut(s)	217
MaeIII	GTNAC	7 cut(s)	94, 248, 338, 472, 1246, 1337, 1357
MalI	GATC	3 cut(s)	443, 746, 977
MboI	GATC	3 cut(s)	441, 744, 975
MboII	GAAGA	9 cut(s)	237, 246, 261, 283, 668, 766, 1004, 1603, 1606
MflI	RGATCY	2 cut(s)	441, 975
MhlI	GDGCHC	2 cut(s)	706, 1318
MlsI	TGGCCA	1 cut(s)	71
MluCI	AATT	8 cut(s)	583, 684, 694, 778, 965, 1182, 1646, 1681
MluNI	TGGCCA	1 cut(s)	71
MmeI	TCCRAC	2 cut(s)	1388, 1443
Mox20I	TGGCCA	1 cut(s)	71
Mph1103I	ATGCAT	1 cut(s)	897
MroI	TCCGGA	2 cut(s)	1393, 1662
MroXI	GAANNNNTTC	2 cut(s)	1560, 1719
MscI	TGGCCA	1 cut(s)	71
MseI	TTAA	8 cut(s)	159, 282, 417, 683, 1104, 1428, 1532, 1698
MslI	CAYNNNNRTG	4 cut(s)	548, 795, 948, 1605
Msp20I	TGGCCA	1 cut(s)	71
MspA1I	CMGCKG	1 cut(s)	614
MspI	CCGG	3 cut(s)	1060, 1394, 1663
MspR9I	CCNGG	3 cut(s)	105, 246, 1061
Mva1269I	GAATGC	1 cut(s)	1502
MvaI	CCWGG	2 cut(s)	105, 246
MwoI	GCNNNNNNNGC	8 cut(s)	227, 614, 916, 1056, 1070, 1079, 1442, 1508
NciI	CCSGG	1 cut(s)	1061
NcoI	CCATGG	3 cut(s)	360, 492, 979
NdeI	CATATG	1 cut(s)	598
NdeII	GATC	3 cut(s)	441, 744, 975
NlaIV	GGNNCC	1 cut(s)	977
NmuCI	GTSAC	6 cut(s)	94, 248, 338, 472, 1337, 1357
NsiI	ATGCAT	1 cut(s)	897
NspI	RCATGY	1 cut(s)	1240
OliI	CACNNNNGTG	1 cut(s)	1605
PceI	AGGCCT	1 cut(s)	1050
PciSI	GCTCTTC	1 cut(s)	220
PctI	GAATGC	1 cut(s)	1502
PdmI	GAANNNNTTC	2 cut(s)	1560, 1719
PfeI	GAWTC	1 cut(s)	1567
PflMI	CCANNNNNTGG	1 cut(s)	1370
PkrI	GCNGC	9 cut(s)	6, 13, 16, 277, 616, 619, 828, 921, 1546
PshAI	GACNNNNGTC	1 cut(s)	1281
PsiI	TTATAA	1 cut(s)	573
Psp6I	CCWGG	2 cut(s)	103, 244
PspGI	CCWGG	2 cut(s)	103, 244
PspN4I	GGNNCC	1 cut(s)	977
PspPI	GGNCC	3 cut(s)	242, 1062, 1373
PstI	CTGCAG	2 cut(s)	13, 619
PstNI	CAGNNNCTG	1 cut(s)	245
PsuI	RGATCY	2 cut(s)	441, 975
PvuII	CAGCTG	1 cut(s)	614
RsaI	GTAC	5 cut(s)	186, 591, 1205, 1347, 1525
RsaNI	GTAC	5 cut(s)	185, 590, 1204, 1346, 1524
RseI	CAYNNNNRTG	4 cut(s)	548, 795, 948, 1605
SapI	GCTCTTC	1 cut(s)	220
SaqAI	TTAA	8 cut(s)	159, 282, 417, 683, 1104, 1428, 1532, 1698
SatI	GCNGC	9 cut(s)	5, 12, 15, 276, 615, 618, 827, 920, 1545
Sau3AI	GATC	3 cut(s)	441, 744, 975
Sau96I	GGNCC	3 cut(s)	242, 1062, 1373
ScaI	AGTACT	1 cut(s)	591
ScrFI	CCNGG	3 cut(s)	105, 246, 1061
SduI	GDGCHC	2 cut(s)	706, 1318
SfaNI	GCATC	5 cut(s)	31, 212, 400, 519, 790
SfcI	CTRYAG	2 cut(s)	9, 615
SinI	GGWCC	1 cut(s)	1373
SmiMI	CAYNNNNRTG	4 cut(s)	548, 795, 948, 1605
SmlI	CTYRAG	1 cut(s)	1135
SmoI	CTYRAG	1 cut(s)	1135
Sse9I	AATT	8 cut(s)	583, 684, 694, 778, 965, 1182, 1646, 1681
SseBI	AGGCCT	1 cut(s)	1050
SsiI	CCGC	4 cut(s)	259, 826, 960, 1162
SspI	AATATT	2 cut(s)	637, 1196
SspMI	CTAG	2 cut(s)	482, 593
StuI	AGGCCT	1 cut(s)	1050
StyD4I	CCNGG	3 cut(s)	103, 244, 1059
StyI	CCWWGG	3 cut(s)	360, 492, 979
TaiI	ACGT	1 cut(s)	220
TaqI	TCGA	6 cut(s)	123, 294, 1158, 1257, 1552, 1713
TasI	AATT	8 cut(s)	583, 684, 694, 778, 965, 1182, 1646, 1681
TatI	WGTACW	3 cut(s)	184, 589, 1203
TauI	GCSGC	1 cut(s)	829
TfiI	GAWTC	1 cut(s)	1567
Tru1I	TTAA	8 cut(s)	159, 282, 417, 683, 1104, 1428, 1532, 1698
Tru9I	TTAA	8 cut(s)	159, 282, 417, 683, 1104, 1428, 1532, 1698
TscAI	CASTG	9 cut(s)	194, 257, 706, 802, 1213, 1318, 1443, 1545, 1629
TseFI	GTSAC	6 cut(s)	94, 248, 338, 472, 1337, 1357
TseI	GCWGC	8 cut(s)	4, 11, 14, 275, 614, 617, 919, 1544
Tsp45I	GTSAC	6 cut(s)	94, 248, 338, 472, 1337, 1357
TspDTI	ATGAA	5 cut(s)	51, 849, 912, 960, 1495
TspRI	CASTG	9 cut(s)	194, 257, 706, 802, 1213, 1318, 1443, 1545, 1629
Van91I	CCANNNNNTGG	1 cut(s)	1370
VneI	GTGCAC	1 cut(s)	1314
VpaK11BI	GGWCC	1 cut(s)	1373
XagI	CCTNNNNNAGG	1 cut(s)	333
XapI	RAATTY	4 cut(s)	694, 778, 965, 1681
XceI	RCATGY	1 cut(s)	1240
XmnI	GAANNNNTTC	2 cut(s)	1560, 1719
XspI	CTAG	2 cut(s)	482, 593
ZrmI	AGTACT	1 cut(s)	591
Zsp2I	ATGCAT	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.