Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Reverse (-)

30366495 .. 30382821

Gene Structure 16327 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00239750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGTGGAATGTCAGAGAAACCCAGAGGATGATGAGCACATCTCACAAAAGGAAGCTCAACAAGTTGAAGGAGGAGAAGGAAAACGACCTTGTGCTATCAAAAAGCGTTGAGGCGAAATATAAGATTCCAGAGGGTGCACGGGCTCTTCTCCAGTCATCACGAAATATTGGCCAGGTTGAGTCCAGAAAGGTTAAACGCAGCAAAGGTTTACTGCACTTGAAACTTACATCCCATTCTTCCAGTGAAAGTGAACCTGAGTCGGACATAACTCAATCAACACAGGATCAATCGTTAATGGTACAAAAGAACACGTCTACTTGTTCGGACTCCTCTACAATGCTACTTTGTGGCACAAGGCCTCCTGATATTCGTAACAAGTCTTTACCAGAAGATGGAACTTCTAGCATGGATCATAACTGGAGTGAAAGCATAAACTTCATGGATAAAGTAGAACAGAGTCCTGAGTCAAGTTACAGCTCAAAGCTTCCTGCACCAAGTATTCTGAAAGATCCAAAAAATAGGCGGCATGTATCAAGACCTCCTGAAGTGGTAAAGGCAAGGAGTGATATTCCTATTATAATGATGGAGCAAGAGATAATGGAGGCCATAAATGAAAATTCCACTGTTATTATCCGCGGAGAGACTGGATGTGGTAAAACAACCCAAGTTCCACAGTTTCTTTATGAAGTTGGCATTGGTTCAAGTATGTCTCCTCTTGGAAGTGGTATCCTTGGTGTTACTCAACCCCGTCGCATTGCAGTCTATGCTACTGCTAGTCGTGTGGCATATGAGTTTGGTGTACGTGTGGGTGAGGAGGTTGGGTATCAAGTTGGATATGACAAAAAGATAGGCAAAAGTTGCTCTATCAACTTCATGACGGATGGAGTATTGCTACGAGAACTCCAGGGTTCAGCAGGGGGTCTCAGTTATGAGCCTGGGTCCCTGAAGTGGGTTCAGGGTCCTGAGTTCAGACAAATAAAAACAAAAACTTTGGAATGGTGCTGGAATGAGATACTCTGTGATAATCTTGATGAGGTTCATGAGCGGAACTTGAGCACAGATACACTCATTGGAATGCTTTCACGTGTTATCATTTGTCGTCAGAAAGAGTATGAACGTCAGCAGGAGTTTTTGCAGCAGTACTCGCATTTAGGAATATCTATAGATCGGGAGAAACTTGTTTTTCCACTGAAGCTTGTGCTCATGAGTGCTACCATGCCAGTGGAAGACTTCATTTCTTATAGGAAGTTGTTTCCTGACCCCCCACAAGAGATAAATGTTTTGTCTAGACAATTTAAAGTAGACATTCAGTTCTCGACGACAACTAAAGCTGATTATACTAAGGAAGCATGTAAAAAGGTCTTGGCAATTCACAAGAATATGCCACAAGGAGGCATACTTGTGTTTGTCACTGGGTACTGGGAAGTGGAGTGCGAGGTAGATGACGATGAGTTTGGTTCTTCATATGATACAGAAACAGAGAGTGAGCTGGAAATTATTGGTGACGATGGGGATTCATTACGTCAAGATGCCCCAGAAGTCGATGATTCTACTTCTTGCAAAGAACCTATTTCACGAACTCTAGACTCTTGCTCTAGCCAATCCAATCATAGTAGCAGCTTGGGGAAAAAAGATGCAGTTGTAGACGATAGTTTTCCAGGCTCTCTGCGTGTTCTTCCTCTTTATTCCATGCTGCCTGCAGCAGCTCAGCATCGTGTATTTGATAAAGTAAAGGAGGGAGAACGGCTTGTTGTTGTGGCCACCAATGTTGCTGAAACCTCTATCACTATACCTGGGATAAAGTATGTTGTTGATACTGGAAAAGTGAAATCAAAGGAATACAACTTTAAAAATGGAAGTGAAATATATAAAGTACAGTGGATTAGTAAGGCGTCGGCTGTTCAACGTGCAGGAAGAGCTGGAAGAACAGGGCCTGGTCACTGTTTCCGCCTTTATTCTTCAGCAGCATTTAATAATTTCTTTGATGACAGTTCCCCATCTGAAATATCCAAAGTCCCTCTTGAAGGTCTTATCCTTCTTTTGAAGTCCATGGACATTGAGTTGTCTGCTTTTCCTTTTCTTACTCGTCCAAAGGATGATGCTCTTAATGGAAGACAGCGTTGCTTGGAGATCCTTGAAGCGCTTACTGAGGATGGAGAAGTGACACCTCTAGGGAAGGCCATGGCTTATTACCCCATAAGTCCTCGCCACTCTAAGATGCTCCTAACAGTCATCAAAATGTTGAATAAGGAGAAAAGTTATAAACGACCTAATTTAGTACTAGCATATGCAGTTGCTTCAGCTGCAGCTTTGAGTTTGTCAAATATTTTTGTTAGTCAGTTTGACGACAGTTGCACAGAAAACCATGACTTAATTGATGGAAATTCCAGTGCTCCAGTTCACGGTGAAGTTATAGACAAGCAAGAGAGAGTGGTCAAAGAGAAGAAAGTGAGAGCGTCATGTGGAAATTTTGGTAGTCATAGCAGTGATGCCCTTTCCAGAGCTTATGTTTTGCGGCTCTATGAACTTTCCAAGAGCCGATTGCATTTCTGCAAGGACAATGCTCTCCACCCAGAAACCATGCGTGAAATATCCGAGCTGAGAGAGCAGCTACTCAAACTTGTCTTTTATTATAGTGATGTGTCTGGCGGTGAATTTTCATGGATCCATGGTAGTCAGGAAGATGTTGAGCATGATTGGAGGGCTGATGTTATCCCTCTTTCTTTGGATGAGGAAGGCCTCTTGTGCCAGGCCATCTGTGCTGGTTGGGCAGATAGAGTTGCCAAGCGCATTAAAGGAAGTAAAAATCAATATCAAACCTGCTTGAGTAATGAGAATGTCTTCCTCGACCGCCAGTCATCTGTTTCTAAAATTGCTCCTGAATATTTGGTGTATAGTGAGTTGATACAGAAAAAGAAACCATACATGCACGGTGTTACTTGTATCGAACCCGAATGGCTTGTTGACTGTGGTCAGGTCTTATGCAAGCTTCCAAAGGGCGGTGCACCTTACTATGACCGTGTAAGTGACAAGGTACTATACAATGTCACTCCAACCTTTGGTCCTCATTTGTGGAAGCTTCCGGAACATTGTTTGCCTATTGATAAGTCTGAGTTTAAAGGTGCAGTGCAAGCTTTTGCTTTGTTGGACGGCAAGGTTCTGCATTGCCTGAGACCTGTGCGTGCATTCATGGCAGAACATCCTTGTACTGTTTTAAAAGCGGTGGCAGCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1078

Amino Acids

120.63

Weight (kDa)

6.24

Isoelectric Point (pI)

46.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	555 - 644	3.3e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	707 - 733	1.5e-06	Helicase associated domain (HA2), winged-helix
OB_NTP_bind	PF07717	915 - 990	4e-18	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	578, 2262
Acc36I	ACCTGC	1 cut(s)	2828
AccB7I	CCANNNNNTGG	2 cut(s)	392, 3059
AccBSI	CCGCTC	1 cut(s)	1045
AccI	GTMKAC	3 cut(s)	314, 1302, 1644
AccII	CGCG	1 cut(s)	636
AccIII	TCCGGA	1 cut(s)	3082
AclWI	GGATC	6 cut(s)	291, 417, 503, 2125, 2659, 2672
AcoI	YGGCCR	2 cut(s)	169, 1758
AcsI	RAATTY	4 cut(s)	616, 2383, 2467, 2654
AcuI	CTGAAG	5 cut(s)	564, 965, 1211, 1944, 2283
AcvI	CACGTG	1 cut(s)	1085
AcyI	GRCGYC	1 cut(s)	1892
AfaI	GTAC	8 cut(s)	300, 801, 1142, 1418, 1875, 2280, 3036, 3208
AfeI	AGCGCT	1 cut(s)	2142
AfiI	CCNNNNNNNGG	8 cut(s)	392, 948, 949, 1391, 2024, 2402, 2999, 3059
AflIII	ACRYGT	3 cut(s)	309, 802, 1084
AgsI	TTSAA	8 cut(s)	67, 220, 702, 1904, 2024, 2044, 2138, 2245
AhdI	GACNNNNNGTC	1 cut(s)	2854
AjiI	CACGTC	1 cut(s)	312
AjnI	CCWGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
Alw21I	GWGCWC	6 cut(s)	38, 139, 1058, 1203, 2395, 3007
Alw26I	GTCTC	4 cut(s)	635, 714, 926, 3166
Alw44I	GTGCAC	2 cut(s)	135, 3003
AlwI	GGATC	6 cut(s)	291, 417, 503, 2125, 2659, 2672
AlwNI	CAGNNNCTG	1 cut(s)	1934
Aor13HI	TCCGGA	1 cut(s)	3082
Aor51HI	AGCGCT	1 cut(s)	2142
AoxI	GGCC	8 cut(s)	169, 356, 603, 1758, 1931, 2178, 2737, 2751
ApaLI	GTGCAC	2 cut(s)	135, 3003
ApoI	RAATTY	4 cut(s)	616, 2383, 2467, 2654
ArsI	GACNNNNNNTTYG	2 cut(s)	1436, 1468
Asp700I	GAANNNNTTC	2 cut(s)	1078, 2840
AspLEI	GCGC	2 cut(s)	2143, 2790
AspS9I	GGNCC	4 cut(s)	939, 959, 1931, 3062
AsuHPI	GGTGA	4 cut(s)	821, 1514, 2417, 2663
AvaII	GGWCC	3 cut(s)	939, 959, 3062
BaeGI	GKGCMC	2 cut(s)	139, 3007
BalI	TGGCCA	2 cut(s)	171, 1760
BamHI	GGATCC	1 cut(s)	2664
BanII	GRGCYC	1 cut(s)	145
BbrPI	CACGTG	1 cut(s)	1085
BbsI	GAAGAC	3 cut(s)	1233, 2119, 2833
Bbv12I	GWGCWC	6 cut(s)	38, 139, 1058, 1203, 2395, 3007
BccI	CCATC	8 cut(s)	386, 577, 875, 1502, 2005, 2147, 2372, 2762
BceAI	ACGGC	2 cut(s)	1760, 3166
BciT130I	CCWGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
BciVI	GTATCC	1 cut(s)	737
BcoDI	GTCTC	4 cut(s)	635, 714, 926, 3166
BfaI	CTAG	7 cut(s)	402, 774, 1287, 1583, 1596, 2171, 2282
BfmI	CTRYAG	3 cut(s)	1161, 1698, 2304
BfoI	RGCGCY	1 cut(s)	2144
BfuAI	ACCTGC	1 cut(s)	2828
BfuI	GTATCC	1 cut(s)	737
BlpI	GCTNAGC	1 cut(s)	1707
BmcAI	AGTACT	2 cut(s)	1142, 2280
Bme1390I	CCNGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
Bme18I	GGWCC	3 cut(s)	939, 959, 3062
BmeRI	GACNNNNNGTC	1 cut(s)	2854
BmgBI	CACGTC	1 cut(s)	312
BmgT120I	GGNCC	4 cut(s)	939, 959, 1931, 3062
BmiI	GGNNCC	4 cut(s)	940, 941, 960, 2666
BmrFI	CCNGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
BmrI	ACTGGG	2 cut(s)	1422, 1429
BmsI	GCATC	6 cut(s)	1519, 1624, 1720, 2089, 2208, 2479
BmuI	ACTGGG	2 cut(s)	1422, 1429
BoxI	GACNNNNGTC	1 cut(s)	2970
BpiI	GAAGAC	3 cut(s)	1233, 2119, 2833
BplI	GAGNNNNNCTC	2 cut(s)	25, 57
BpmI	CTGGAG	4 cut(s)	134, 439, 887, 2379
Bpu1102I	GCTNAGC	1 cut(s)	1707
BpuEI	CTTGAG	2 cut(s)	1072, 2845
BsaAI	YACGTR	2 cut(s)	803, 1085
BsaHI	GRCGYC	1 cut(s)	1892
BsaI	GGTCTC	2 cut(s)	926, 3166
BsaJI	CCNNGG	8 cut(s)	634, 730, 904, 935, 1793, 2049, 2181, 2668
BsaWI	WCCGGW	1 cut(s)	3082
BsaXI	ACNNNNNCTCC	6 cut(s)	343, 373, 806, 836, 885, 915
Bsc4I	CCNNNNNNNGG	8 cut(s)	392, 948, 949, 1391, 2024, 2402, 2999, 3059
Bse3DI	GCAATG	2 cut(s)	753, 3163
BseAI	TCCGGA	1 cut(s)	3082
BseBI	CCWGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
BseDI	CCNNGG	8 cut(s)	634, 730, 904, 935, 1793, 2049, 2181, 2668
BseGI	GGATG	8 cut(s)	33, 227, 653, 886, 2101, 2158, 2734, 3199
BseLI	CCNNNNNNNGG	8 cut(s)	392, 948, 949, 1391, 2024, 2402, 2999, 3059
BseMI	GCAATG	2 cut(s)	753, 3163
BseMII	CTCAG	9 cut(s)	246, 453, 937, 954, 1721, 2139, 2591, 3102, 3161
BseRI	GAGGAG	4 cut(s)	86, 319, 702, 827
BseSI	GKGCMC	2 cut(s)	139, 3007
BsgI	GTGCAG	4 cut(s)	197, 474, 1929, 3144
Bsh1236I	CGCG	1 cut(s)	636
Bsh1285I	CGRYCG	1 cut(s)	2851
BshFI	GGCC	8 cut(s)	171, 358, 605, 1760, 1933, 2180, 2739, 2753
BsiEI	CGRYCG	1 cut(s)	2851
BsiHKAI	GWGCWC	6 cut(s)	38, 139, 1058, 1203, 2395, 3007
BsiSI	CCGG	1 cut(s)	3083
BslFI	GGGAC	2 cut(s)	925, 2000
BslI	CCNNNNNNNGG	8 cut(s)	392, 948, 949, 1391, 2024, 2402, 2999, 3059
BsmAI	GTCTC	4 cut(s)	635, 714, 926, 3166
BsmFI	GGGAC	2 cut(s)	925, 2000
BsmI	GAATGC	2 cut(s)	1080, 3185
BsnI	GGCC	8 cut(s)	171, 358, 605, 1760, 1933, 2180, 2739, 2753
Bso31I	GGTCTC	2 cut(s)	926, 3166
Bsp1286I	GDGCHC	7 cut(s)	38, 139, 145, 1058, 1203, 2395, 3007
Bsp13I	TCCGGA	1 cut(s)	3082
Bsp143I	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
Bsp1720I	GCTNAGC	1 cut(s)	1707
Bsp19I	CCATGG	3 cut(s)	2049, 2181, 2668
BspANI	GGCC	8 cut(s)	171, 358, 605, 1760, 1933, 2180, 2739, 2753
BspCNI	CTCAG	9 cut(s)	247, 454, 936, 955, 1720, 2140, 2592, 3103, 3162
BspEI	TCCGGA	1 cut(s)	3082
BspFNI	CGCG	1 cut(s)	636
BspHI	TCATGA	3 cut(s)	873, 1039, 1203
BspLI	GGNNCC	4 cut(s)	940, 941, 960, 2666
BspMAI	CTGCAG	2 cut(s)	1702, 2308
BspMI	ACCTGC	1 cut(s)	2828
BspPI	GGATC	6 cut(s)	291, 417, 503, 2125, 2659, 2672
BspQI	GCTCTTC	2 cut(s)	150, 1909
BspTNI	GGTCTC	2 cut(s)	926, 3166
BsrBI	CCGCTC	1 cut(s)	1045
BsrDI	GCAATG	2 cut(s)	753, 3163
BssECI	CCNNGG	8 cut(s)	634, 730, 904, 935, 1793, 2049, 2181, 2668
BssMI	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
BssNI	GRCGYC	1 cut(s)	1892
BssT1I	CCWWGG	4 cut(s)	730, 2049, 2181, 2668
Bst2UI	CCWGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
Bst6I	CTCTTC	2 cut(s)	150, 1909
BstACI	GRCGYC	1 cut(s)	1892
BstAPI	GCANNNNNTGC	2 cut(s)	764, 858
BstBAI	YACGTR	2 cut(s)	803, 1085
BstC8I	GCNNGC	4 cut(s)	1698, 2987, 3132, 3183
BstDSI	CCRYGG	4 cut(s)	634, 2049, 2181, 2668
BstENI	CCTNNNNNAGG	1 cut(s)	2022
BstF5I	GGATG	8 cut(s)	33, 227, 653, 886, 2101, 2158, 2734, 3199
BstFNI	CGCG	1 cut(s)	636
BstH2I	RGCGCY	1 cut(s)	2144
BstHHI	GCGC	2 cut(s)	2143, 2790
BstKTI	GATC	6 cut(s)	286, 412, 511, 1168, 2133, 2667
BstMAI	GTCTC	4 cut(s)	635, 714, 926, 3166
BstMBI	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
BstMCI	CGRYCG	1 cut(s)	2851
BstNI	CCWGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
BstNSI	RCATGY	3 cut(s)	530, 1353, 2929
BstPAI	GACNNNNGTC	1 cut(s)	2970
BstSCI	CCNGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
BstSFI	CTRYAG	3 cut(s)	1161, 1698, 2304
BstSLI	GKGCMC	2 cut(s)	139, 3007
BstUI	CGCG	1 cut(s)	636
BstV2I	GAAGAC	3 cut(s)	1233, 2119, 2833
BstX2I	RGATCY	3 cut(s)	508, 2130, 2664
BstXI	CCANNNNNNTGG	1 cut(s)	1222
BstYI	RGATCY	3 cut(s)	508, 2130, 2664
BsuI	GTATCC	1 cut(s)	737
BsuRI	GGCC	8 cut(s)	171, 358, 605, 1760, 1933, 2180, 2739, 2753
BtgI	CCRYGG	4 cut(s)	634, 2049, 2181, 2668
BtrI	CACGTC	1 cut(s)	312
BtsCI	GGATG	8 cut(s)	33, 227, 653, 886, 2101, 2158, 2734, 3199
BtsI	GCAGTG	2 cut(s)	2491, 3132
BveI	ACCTGC	1 cut(s)	2828
Cac8I	GCNNGC	4 cut(s)	1698, 2987, 3132, 3183
CaiI	CAGNNNCTG	1 cut(s)	1934
CciI	TCATGA	3 cut(s)	873, 1039, 1203
CfoI	GCGC	2 cut(s)	2143, 2790
Cfr13I	GGNCC	4 cut(s)	939, 959, 1931, 3062
Cfr42I	CCGCGG	1 cut(s)	637
CseI	GACGC	2 cut(s)	1881, 2445
Csp6I	GTAC	8 cut(s)	299, 800, 1141, 1417, 1874, 2279, 3035, 3207
CspCI	CAANNNNNGTGG	2 cut(s)	1750, 1785
CviQI	GTAC	8 cut(s)	299, 800, 1141, 1417, 1874, 2279, 3035, 3207
DpnI	GATC	6 cut(s)	285, 411, 510, 1167, 2132, 2666
DpnII	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
DraI	TTTAAA	4 cut(s)	1297, 1849, 3118, 3216
DriI	GACNNNNNGTC	1 cut(s)	2854
EaeI	YGGCCR	2 cut(s)	169, 1758
Eam1104I	CTCTTC	2 cut(s)	150, 1909
Eam1105I	GACNNNNNGTC	1 cut(s)	2854
EarI	CTCTTC	2 cut(s)	150, 1909
EciI	GGCGGA	1 cut(s)	1937
Eco130I	CCWWGG	4 cut(s)	730, 2049, 2181, 2668
Eco147I	AGGCCT	2 cut(s)	358, 2739
Eco24I	GRGCYC	1 cut(s)	145
Eco31I	GGTCTC	2 cut(s)	926, 3166
Eco47I	GGWCC	3 cut(s)	939, 959, 3062
Eco47III	AGCGCT	1 cut(s)	2142
Eco57I	CTGAAG	5 cut(s)	564, 965, 1211, 1944, 2283
Eco72I	CACGTG	1 cut(s)	1085
EcoNI	CCTNNNNNAGG	1 cut(s)	2022
EcoO109I	RGGNCCY	3 cut(s)	939, 959, 1931
EcoRII	CCWGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
EcoT14I	CCWWGG	4 cut(s)	730, 2049, 2181, 2668
EcoT38I	GRGCYC	1 cut(s)	145
ErhI	CCWWGG	4 cut(s)	730, 2049, 2181, 2668
FalI	AAGNNNNNCTT	2 cut(s)	2004, 2036
FaqI	GGGAC	2 cut(s)	925, 2000
FauNDI	CATATG	3 cut(s)	787, 1465, 2287
FblI	GTMKAC	3 cut(s)	314, 1302, 1644
FokI	GGATG	8 cut(s)	40, 214, 660, 893, 2108, 2165, 2741, 3186
FriOI	GRGCYC	1 cut(s)	145
FspBI	CTAG	7 cut(s)	402, 774, 1287, 1583, 1596, 2171, 2282
GlaI	GCGC	2 cut(s)	2142, 2789
GsuI	CTGGAG	4 cut(s)	134, 439, 887, 2379
HaeII	RGCGCY	1 cut(s)	2144
HaeIII	GGCC	8 cut(s)	171, 358, 605, 1760, 1933, 2180, 2739, 2753
HapII	CCGG	1 cut(s)	3083
HgaI	GACGC	2 cut(s)	1881, 2445
HhaI	GCGC	2 cut(s)	2143, 2790
Hin1I	GRCGYC	1 cut(s)	1892
Hin6I	GCGC	2 cut(s)	2141, 2788
HinP1I	GCGC	2 cut(s)	2141, 2788
HincII	GTYRAC	1 cut(s)	2965
HindII	GTYRAC	1 cut(s)	2965
HindIII	AAGCTT	5 cut(s)	482, 1193, 2987, 3077, 3132
HinfI	GANTC	9 cut(s)	124, 179, 257, 326, 457, 464, 1514, 1547, 1586
HpaII	CCGG	1 cut(s)	3083
HphI	GGTGA	4 cut(s)	821, 1514, 2417, 2663
Hpy188I	TCNGA	9 cut(s)	14, 262, 325, 504, 971, 1104, 2002, 2596, 3112
Hpy99I	CGWCG	3 cut(s)	753, 1321, 1897
HpyAV	CCTTC	6 cut(s)	61, 70, 2018, 2045, 2170, 2729
HpyCH4IV	ACGT	6 cut(s)	311, 802, 1084, 1117, 1522, 1906
HpySE526I	ACGT	6 cut(s)	311, 802, 1084, 1117, 1522, 1906
Hsp92I	GRCGYC	1 cut(s)	1892
HspAI	GCGC	2 cut(s)	2141, 2788
KflI	GGGWCCC	1 cut(s)	939
Kpn2I	TCCGGA	1 cut(s)	3082
KspI	CCGCGG	1 cut(s)	637
Kzo9I	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
LguI	GCTCTTC	2 cut(s)	150, 1909
LmnI	GCTCC	4 cut(s)	586, 2226, 2398, 2881
LweI	GCATC	6 cut(s)	1519, 1624, 1720, 2089, 2208, 2479
MaeI	CTAG	7 cut(s)	402, 774, 1287, 1583, 1596, 2171, 2282
MaeII	ACGT	6 cut(s)	311, 802, 1084, 1117, 1522, 1906
MalI	GATC	6 cut(s)	285, 411, 510, 1167, 2132, 2666
MbiI	CCGCTC	1 cut(s)	1045
MboI	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
MflI	RGATCY	3 cut(s)	508, 2130, 2664
MhlI	GDGCHC	7 cut(s)	38, 139, 145, 1058, 1203, 2395, 3007
MlsI	TGGCCA	2 cut(s)	171, 1760
MluNI	TGGCCA	2 cut(s)	171, 1760
MlyI	GAGTC	6 cut(s)	188, 266, 320, 466, 473, 1580
MmeI	TCCRAC	4 cut(s)	240, 811, 3077, 3126
Mox20I	TGGCCA	2 cut(s)	171, 1760
MroI	TCCGGA	1 cut(s)	3082
MroXI	GAANNNNTTC	2 cut(s)	1078, 2840
MscI	TGGCCA	2 cut(s)	171, 1760
MslI	CAYNNNNRTG	5 cut(s)	1073, 1220, 1400, 2237, 2484
Msp20I	TGGCCA	2 cut(s)	171, 1760
MspA1I	CMGCKG	2 cut(s)	636, 2303
MspI	CCGG	1 cut(s)	3083
MspR9I	CCNGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
Mva1269I	GAATGC	2 cut(s)	1080, 3185
MvaI	CCWGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
MvnI	CGCG	1 cut(s)	636
NcoI	CCATGG	3 cut(s)	2049, 2181, 2668
NdeI	CATATG	3 cut(s)	787, 1465, 2287
NdeII	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
NlaIV	GGNNCC	4 cut(s)	940, 941, 960, 2666
NmuCI	GTSAC	6 cut(s)	1408, 1502, 1937, 2161, 3026, 3046
NspI	RCATGY	3 cut(s)	530, 1353, 2929
PagI	TCATGA	3 cut(s)	873, 1039, 1203
PceI	AGGCCT	2 cut(s)	358, 2739
PciSI	GCTCTTC	2 cut(s)	150, 1909
PctI	GAATGC	2 cut(s)	1080, 3185
PdmI	GAANNNNTTC	2 cut(s)	1078, 2840
PfeI	GAWTC	3 cut(s)	124, 1514, 1547
PflMI	CCANNNNNTGG	2 cut(s)	392, 3059
PleI	GAGTC	6 cut(s)	187, 265, 320, 465, 472, 1580
PmaCI	CACGTG	1 cut(s)	1085
PmlI	CACGTG	1 cut(s)	1085
PpsI	GAGTC	6 cut(s)	187, 265, 320, 465, 472, 1580
Ppu21I	YACGTR	2 cut(s)	803, 1085
PpuMI	RGGWCCY	2 cut(s)	939, 959
PshAI	GACNNNNGTC	1 cut(s)	2970
PsiI	TTATAA	2 cut(s)	578, 2262
Psp5II	RGGWCCY	2 cut(s)	939, 959
Psp6I	CCWGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
PspCI	CACGTG	1 cut(s)	1085
PspGI	CCWGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
PspN4I	GGNNCC	4 cut(s)	940, 941, 960, 2666
PspPI	GGNCC	4 cut(s)	939, 959, 1931, 3062
PspPPI	RGGWCCY	2 cut(s)	939, 959
PstI	CTGCAG	2 cut(s)	1702, 2308
PstNI	CAGNNNCTG	1 cut(s)	1934
PsuI	RGATCY	3 cut(s)	508, 2130, 2664
PvuII	CAGCTG	1 cut(s)	2303
RsaI	GTAC	8 cut(s)	300, 801, 1142, 1418, 1875, 2280, 3036, 3208
RsaNI	GTAC	8 cut(s)	299, 800, 1141, 1417, 1874, 2279, 3035, 3207
RseI	CAYNNNNRTG	5 cut(s)	1073, 1220, 1400, 2237, 2484
SacII	CCGCGG	1 cut(s)	637
SapI	GCTCTTC	2 cut(s)	150, 1909
Sau3AI	GATC	6 cut(s)	283, 409, 508, 1165, 2130, 2664
Sau96I	GGNCC	4 cut(s)	939, 959, 1931, 3062
ScaI	AGTACT	2 cut(s)	1142, 2280
SchI	GAGTC	6 cut(s)	188, 266, 320, 466, 473, 1580
ScrFI	CCNGG	7 cut(s)	173, 905, 936, 1659, 1794, 1935, 2750
SduI	GDGCHC	7 cut(s)	38, 139, 145, 1058, 1203, 2395, 3007
SfaNI	GCATC	6 cut(s)	1519, 1624, 1720, 2089, 2208, 2479
SfcI	CTRYAG	3 cut(s)	1161, 1698, 2304
Sfr303I	CCGCGG	1 cut(s)	637
SgrBI	CCGCGG	1 cut(s)	637
SinI	GGWCC	3 cut(s)	939, 959, 3062
SmiMI	CAYNNNNRTG	5 cut(s)	1073, 1220, 1400, 2237, 2484
SmlI	CTYRAG	2 cut(s)	1051, 2824
SmoI	CTYRAG	2 cut(s)	1051, 2824
SseBI	AGGCCT	2 cut(s)	358, 2739
SspI	AATATT	3 cut(s)	166, 2326, 2885
SspMI	CTAG	7 cut(s)	402, 774, 1287, 1583, 1596, 2171, 2282
StuI	AGGCCT	2 cut(s)	358, 2739
StyD4I	CCNGG	7 cut(s)	171, 903, 934, 1657, 1792, 1933, 2748
StyI	CCWWGG	4 cut(s)	730, 2049, 2181, 2668
TaiI	ACGT	6 cut(s)	314, 805, 1087, 1120, 1525, 1909
TaqI	TCGA	4 cut(s)	1316, 1542, 2847, 2946
TatI	WGTACW	4 cut(s)	1140, 1873, 2278, 3206
TauI	GCSGC	2 cut(s)	526, 2518
TfiI	GAWTC	3 cut(s)	124, 1514, 1547
TseFI	GTSAC	6 cut(s)	1408, 1502, 1937, 2161, 3026, 3046
Tsp45I	GTSAC	6 cut(s)	1408, 1502, 1937, 2161, 3026, 3046
TspGWI	ACGGA	1 cut(s)	893
Van91I	CCANNNNNTGG	2 cut(s)	392, 3059
VneI	GTGCAC	2 cut(s)	135, 3003
VpaK11BI	GGWCC	3 cut(s)	939, 959, 3062
XagI	CCTNNNNNAGG	1 cut(s)	2022
XapI	RAATTY	4 cut(s)	616, 2383, 2467, 2654
XbaI	TCTAGA	2 cut(s)	1286, 1582
XceI	RCATGY	3 cut(s)	530, 1353, 2929
XmiI	GTMKAC	3 cut(s)	314, 1302, 1644
XmnI	GAANNNNTTC	2 cut(s)	1078, 2840
XspI	CTAG	7 cut(s)	402, 774, 1287, 1583, 1596, 2171, 2282
ZrmI	AGTACT	2 cut(s)	1142, 2280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.