Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0000599

Physical Location & Seq

Genomic Coordinates Forward (+)

37040363 .. 37047170

Gene Structure 6808 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG146500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3975 bp

ATGGAAAATTTTGAACAGCCTATGGACTTGAGCTCTGGAGGTGGCGATAGTAATGCAATCGTCTTGCCAAATAAGAGAAAGAGGAAAAGGAAGGGTGCTACTCAGGAGGGTAAGATACTTGGCACAAGAAAAACACCAAAGATGACCAAATCCCAGGAAAGGAAGTTGCAAAAGTTGGAGGAGGAGAAGGAAAAAGCCCTTTTGCTATCCAAGAGCATTGATGCCTTAGGGAAATATAAGCTTCCGGAGGGTGCATATTCTCTTTTGCAATCATCAAAAGATATTGGCCAGGTTGAATCAAAAAAGGAAAAACGTAGGAAGGCTGTACTATTCTCCAAAGCTGGATTAGAAGTTCCCTATACCAACCAACCATTAAAAAAGAAAGACGGTATGGATGCTTGTAGTGAAAGTGAACCTGAGTCGGAAAGAACTCAACCAAGGCAGGATCTTGACAAAAGTGGTCAAGTACAACCGATGGTGATACAAAAGGAAATCCAAAAGACCACATCTGTATTTTTGAACTCCTCGCAGGGGCTAGTCTATAGCAGAGGACATGCTACAAATGGTGGTCCTGCTGATGGCTCACTGTCTAAAAATGTTATTTGCAACGTGTCTTTGCCAGAAAACATAGCTTCTAGTGTGAATGATGACCATGAAAGAACAGAGGCAATGGATAGAGTAAAAGGGAGCCCAAAGGTGACTTCAAGCAGGACAGGTGAATTTTCTGATTTTGCTGCTCCAAGGTCTTTGATCGCTCCAACTATAGTGCACGTATCAAGGCCAATTGAAGTTGAAAACACAAGGAAGGATCTTCCAATAGTAATGATGGAACAGGAGATAATGGAGGCTGTTAATGACCATTCCACTGTTATTATATGTGGAGAAACAGGGTGTGGTAAAACCACTCAGGTTCCTCAGTTTCTTTTTGAAGCTGGCTTTGGTTCAAGTCACTCTTGTCATCGAAGCGGTATTATTGGTGTCACTCAACCTCGTCGTGTTGCAGTCCTTGCAACTGCTAAGCGAGTTGCATATGAGCTTGGTCTGCATTTAGGTAAAGAGGTTGGCTTTCAAGTTAGGTTTGACAAAAAATTTGGCGACAGTTCCTCCATCAAGTTCATGACAGATGGAATATTACTACGCGAGCTTCAGAGTGATTTTTTGTTAAAGCGATACTCTATAATAATTCTTGATGAAGCTCATGAGCGGAGCTTGAGCACGGACATACTTATTGGAATGCTTTCTCGTGTAATTAGTACTCGCCAGGAAATCTATGTTAAGCAGCAAAGGGCTGTGCTTTCAGGAAAAACTATAGATCCTGGACAACAAGTTTTTCCATTAAAGCTTGTGCTGATGAGTGCCACCTTGCAAGTGGAAGACTTCATTTCTGGTAGAAAGTTGTTTCGTGATGTCCCGCCAGTTATAGAAGTTCCCACTAGACAATTTCCAGTAACCACTCACTTCTCAAAAAGAACTGGGACGGACTATGTTGGCCAAGCATATAAAAAAGTCTTGGCAATTCACAAGAGGCTGCCCCGTGGGGGCATACTTGTCTTTGTCACTGGGCAGAAGGAAGTAGAGTTCTTGTGTCAAAAACTACGTAGAGCTTCAACAGAACTGGTCATGAAAACATCTAGAGGGAAAATTGGGTGTAATGCCACTGATGCTTCTAATAAAGATGTTGAACTACTAGATATGAACGAGATTAATGAAGCATTTGAGGTTGAAGAAAATTTAGCCGACCATCAAATTGACAGGTTTAGCTCTGTTGATGAAGTTCTGGGTAATATATATGAGGATGAATTGGATGCTTCGTATGATTCAGACACAGACAGTGAAGTGGAAATTATTGATGATGATGGGGGTTTATTGTTACACAATCCCCCAGAAATTGATGGTGTTGGTGCAGATGTTTTAGGAGAGACGGGGAGCATTGCTTCACTAAAAGCTGCTTTTGAAGCTTTGGCTGGTAAAACTGAATTGTACTCTAGTTCTGATGGGAAACAGCCAATTTCAGTTACTGTAGATGCTTACTTTAATAAATCCCATCCTAGTCTTGGGAAAAAGAGTGAGGTTGATATTGATAATTCCCCAGGTGGCTTGCATGTTCTCCCTCTTTATGCCATGCTGCCTGCGGCTTCTCAGCTTCGCGTATTTGAAGAAGTCAAGGAAGGAGATCGACTAGTTGTTGTAGCCACAAATGTGGCTGAGACCTCTTTAACCATACCTGGGGTAAAGTATGTCGTCGATACTGGCAGAGTAAAAGTGAAAAACTACAATTCCTCAAATGGTATGGAAACATATAACATAAAGTGGATAAGTAAGGCATCAGCTGCTCAACGTAAAGGAAGAGCTGGAAGAACAGGACCCGGGCACTGTTATCGTCTTTATTCTTCTGCAGTATATAATAATGAATTTCCCGACTTTTCTCCGGCTGAAATATTGAAAGTCCCTATTGATGGCGTTCTCCTTCTCATGAAATCTATGAATATTGAAAAGGTATCCAATTTTCCATTTCCAACCCCACCTGATGTTGCTGCCTTGGGTGAAGCAGAACGTTGCTTGAATGCCCTTGAAGCACTTGACAGCAATGGCAGATTAACACCTTTAGGGAAGGCCATGGCTCGTTACCCCATGAGTCCTCGCCACTCCAGGATGCTCCTTACGGTTATTCAAATCATGTACAATGAGAAAAGTTATTCCCGCAGTAAATTAATTCTAGCATATGCAGTTGCAGCAGCGGCTGCTTTGAGTTTGTCAAATCCTTTTGTGAGGCAGTTTGAAAATGACTCAAATAACCATGACTTGGATCAGGATGAAAATCTCAGCGCTTCTGTGAACAATAAGGTGTTAGAGAAGCAAGAAAAGTTAAGAAGGAAGAAACTAAAAGAAACTGTAAAGGTGTTTCGTGAAAAATTTTCTAATCCTGTCAGTGATGCTCTGTCTGTAGCTTATGCTTTGCAGTGCTATGAACTTTCTAGGAACCCACTAAAGTTCTGCAATGATAATGCCCTACACCCAAAAACGATGGAGGAAATGTCCAAGCTGAGAAAGCAACTGCTCCAACTTGTATTTAGTCAGTGTGGTGTATCTGGCAGTGAGAAGGATTTTTCATGCATTTTTGGAAGTTTGGAAGATGTTGAACATGTTTGGAGGGCTTCCCATGATAAGAGTCCTCTTTCACTGTATGAGGAAGATCTCTTAGGCCAAGCTATCTGTGCTGGTTGGGCAGATAGGGTGGCGAAACGTATTAGAGGAACCTCAGGGTCATCTGAAGTAGATAGAAAAGTTCATGCAGTGCGGTATCAAGCCTGCATGGTTGAAGAGACTGTGTTCCTCCACCGTTGGTCATCTGTTTCTAATGCTGCCCCAGAGTTTTTGGTGTACACTGAGCTAATACAGACAAGACGTCCATACATGCATGGTGTTACTAGTGTAAAACCAGAATGGCTTGTAAAGTATGCTCCGTCTTTATGCACTTTCTCTTCAGCTTCATCCGATGCCAAGCAGTATTATGAGCCTGTGACTGACCAAGTGTTACATGATGTCATTCCAGCCTTTGGTCCTCATCTATGGAAGCTTCCCCCACATAGTTTGCCTATTACTAACTATATCTCTCGAGTGGCAGTTTTTGCTTATGCTTTGCTTGAAGGCCAGGTTTTGCCATGCTTGAGATGTGCTCGTGAGTTTATGAAGGCACCTCCAGCTAGTATTTTACGGCCAGAGGCAGAAGGGCAAAGACGGGCTGGAAATTTGTTAGCTAAGTTAAACATAAAAAAGATGGACAGCTGTGCTATGTTAAGAGAGATCTGGAAGGAGAATCCTAATGAATTGCATTCAGAAATCCAGGATTGGTTTAAGGAGAGTTTCCGCAAGAATTTGGCAGCTCTATGGTCAGACATGCTTAGGGAAGTTCTTTTAGAGCCGCAAGAACGGTTTTCAAAGAGAGAGATCAGAAGAATTGAAGTTAAAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1324

Amino Acids

147.63

Weight (kDa)

8.33

Isoelectric Point (pI)

47.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	699 - 788	3.8e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	850 - 877	3.2e-09	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	879 - 981	2.6e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	1068 - 1156	5.7e-19	Oligonucleotide/oligosaccharide-binding (OB)-fold
DHX37_C	PF23362	1226 - 1299	3.7e-16	DEAH helicase DHX37 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33390 AT1G33390
fragaria_vesca	FvH4_3g40090 FvH4_3g40090 FvH4_3g40090 FvH4_6g48600
malus_domestica	MD04G1241500.v1.1 MD05G1017800.v1.1
prunus_persica	Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1 Prupe.1G193500_v2.0.a1
pyrus_communis	pycom04g21310 pycom05g01000 pycom05g01050 pycom11g20470
rosa_chinensis	RchiOBHm_Chr2g0168421 RchiOBHm_Chr2g0168431 RchiOBHm_Chr2g0168441 RchiOBHm_Chr2g0168451 RchiOBHm_Chr2g0168461 RchiOBHm_Chr4g0407811 RchiOBHm_Chr7g0220161 RchiOBHm_Chr7g0220171 RchiOBHm_Chr7g0220481
rosa_laevigata	RLG00000002253 RLG00000008696 RLG00000021781
rosa_multiflora	Rmu_co8232135.1_g000001 Rmu_sc0000966.1_g000007 Rmu_sc0003573.1_g000032 Rmu_sc0006361.1_g000012 Rmu_sc0006361.1_g000013 Rmu_sc0012607.1_g000001 Rmu_sc0016657.1_g000009
rosa_roxburghii	Rroxscaffold_2G00083000 Rroxscaffold_2G00083010 Rroxscaffold_3G00239750 Rroxscaffold_5G00352370
rosa_rugosa	Rorug02G0533800 Rorug02G0533800 Rorug04G0081400 Rorug07G0189900 Rorug07G0190000
rosa_samantha	Rh2AG603500 Rh2AG603700 Rh2BG616600 Rh2BG616700 Rh2BG616800 Rh2CG584500 Rh2CG584600 Rh2CG584700 Rh2DG627100 Rh2DG627200 Rh4AG146500 Rh4BG144000 Rh4CG153000 Rh4DG140500 Rh7AG332900 Rh7BG324300 Rh7BG324400 Rh7BG324500 Rh7CG350400 Rh7DG329000
rosa_wichuraiana	Rw0G022710 Rw2G050050 Rw4G011990 Rw7G028060 Rw7G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3409
AccB1I	GGYRCC	1 cut(s)	3694
AccB7I	CCANNNNNTGG	2 cut(s)	2803, 3557
AccBSI	CCGCTC	1 cut(s)	1204
AccII	CGCG	2 cut(s)	1140, 2148
AccIII	TCCGGA	1 cut(s)	244
AciI	CCGC	9 cut(s)	966, 1204, 1412, 2132, 2701, 2738, 3298, 3868, 3923
AclI	AACGTT	1 cut(s)	2554
AclWI	GGATC	4 cut(s)	453, 816, 1307, 2814
AcoI	YGGCCR	3 cut(s)	286, 1489, 3716
AcsI	RAATTY	8 cut(s)	7, 719, 1088, 1729, 2411, 2912, 3748, 3874
AcuI	CTGAAG	3 cut(s)	1130, 3291, 3468
AcyI	GRCGYC	1 cut(s)	3406
AfaI	GTAC	6 cut(s)	327, 468, 1255, 1982, 2681, 3383
AfeI	AGCGCT	1 cut(s)	2827
AfiI	CCNNNNNNNGG	9 cut(s)	159, 531, 578, 1538, 2054, 2226, 2456, 2803, 3557
AflIII	ACRYGT	2 cut(s)	609, 3142
AhdI	GACNNNNNGTC	1 cut(s)	3405
AhlI	ACTAGT	2 cut(s)	2179, 3428
AjnI	CCWGG	9 cut(s)	153, 288, 1260, 1315, 2089, 2224, 2648, 3651, 3843
AjuI	GAANNNNNNNTTGG	4 cut(s)	685, 717, 921, 953
AleI	CACNNNNGTG	1 cut(s)	2198
AloI	GAACNNNNNNTCC	4 cut(s)	336, 368, 1562, 1594
Alw21I	GWGCWC	4 cut(s)	35, 771, 1217, 3679
Alw26I	GTCTC	3 cut(s)	1913, 2201, 3317
Alw44I	GTGCAC	1 cut(s)	767
AlwI	GGATC	4 cut(s)	453, 816, 1307, 2814
AlwNI	CAGNNNCTG	2 cut(s)	2018, 2741
Ama87I	CYCGRG	2 cut(s)	2366, 3615
Aor13HI	TCCGGA	1 cut(s)	244
Aor51HI	AGCGCT	1 cut(s)	2827
AoxI	GGCC	7 cut(s)	286, 779, 1489, 2613, 3202, 3649, 3716
ApaLI	GTGCAC	1 cut(s)	767
ApoI	RAATTY	8 cut(s)	7, 719, 1088, 1729, 2411, 2912, 3748, 3874
ArsI	GACNNNNNNTTYG	2 cut(s)	1073, 1105
AseI	ATTAAT	2 cut(s)	1704, 2711
Asp700I	GAANNNNTTC	1 cut(s)	1237
AspLEI	GCGC	1 cut(s)	2828
AspS9I	GGNCC	3 cut(s)	569, 2363, 3560
AsuC2I	CCSGG	2 cut(s)	2367, 2368
AsuHPI	GGTGA	4 cut(s)	490, 709, 728, 2555
AvaI	CYCGRG	2 cut(s)	2366, 3615
AvaII	GGWCC	3 cut(s)	569, 2363, 3560
AxyI	CCTNAGG	2 cut(s)	226, 3259
BaeGI	GKGCMC	2 cut(s)	771, 2373
BalI	TGGCCA	2 cut(s)	288, 1491
BanI	GGYRCC	1 cut(s)	3694
BanII	GRGCYC	2 cut(s)	35, 692
BauI	CACGAG	2 cut(s)	1242, 3678
BbsI	GAAGAC	1 cut(s)	1380
Bbv12I	GWGCWC	4 cut(s)	35, 771, 1217, 3679
BceAI	ACGGC	1 cut(s)	3731
BcgI	CGANNNNNNTGC	4 cut(s)	35, 69, 2360, 2394
BciT130I	CCWGG	9 cut(s)	155, 290, 1262, 1317, 2091, 2226, 2650, 3653, 3845
BciVI	GTATCC	1 cut(s)	2509
BcnI	CCSGG	2 cut(s)	2367, 2368
BcoDI	GTCTC	3 cut(s)	1913, 2201, 3317
BcuI	ACTAGT	2 cut(s)	2179, 3428
BfmI	CTRYAG	6 cut(s)	541, 762, 1308, 2019, 2394, 2943
BfoI	RGCGCY	1 cut(s)	2829
BfuI	GTATCC	1 cut(s)	2509
BglII	AGATCT	2 cut(s)	3193, 3804
BlpI	GCTNAGC	1 cut(s)	1017
BmcAI	AGTACT	1 cut(s)	1255
Bme18I	GGWCC	3 cut(s)	569, 2363, 3560
BmeRI	GACNNNNNGTC	1 cut(s)	3405
BmeT110I	CYCGRG	2 cut(s)	2366, 3615
BmgT120I	GGNCC	3 cut(s)	569, 2363, 3560
BmiI	GGNNCC	6 cut(s)	689, 912, 2365, 2981, 3256, 3696
BmrI	ACTGGG	2 cut(s)	1482, 1569
BmsI	GCATC	9 cut(s)	211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487
BmuI	ACTGGG	2 cut(s)	1482, 1569
BpiI	GAAGAC	1 cut(s)	1380
BplI	GAGNNNNNCTC	2 cut(s)	3661, 3693
BpmI	CTGGAG	3 cut(s)	57, 2632, 3684
Bpu10I	CCTNAGC	1 cut(s)	3902
Bpu1102I	GCTNAGC	1 cut(s)	1017
BpuEI	CTTGAG	3 cut(s)	49, 1231, 3688
BpuMI	CCSGG	2 cut(s)	2367, 2368
BsaAI	YACGTR	2 cut(s)	772, 1598
BsaBI	GATNNNNATC	1 cut(s)	2817
BsaHI	GRCGYC	1 cut(s)	3406
BsaI	GGTCTC	1 cut(s)	2201
BsaJI	CCNNGG	9 cut(s)	153, 437, 740, 1534, 2089, 2225, 2366, 2538, 2616
BsaWI	WCCGGW	1 cut(s)	244
BsaXI	ACNNNNNCTCC	4 cut(s)	1088, 1118, 3020, 3050
Bsc4I	CCNNNNNNNGG	9 cut(s)	159, 531, 578, 1538, 2054, 2226, 2456, 2803, 3557
Bse1I	ACTGG	6 cut(s)	1415, 1445, 1477, 1564, 1620, 2254
Bse21I	CCTNAGG	2 cut(s)	226, 3259
Bse3DI	GCAATG	4 cut(s)	675, 1929, 2593, 3004
Bse8I	GATNNNNATC	1 cut(s)	2817
BseAI	TCCGGA	1 cut(s)	244
BseBI	CCWGG	9 cut(s)	155, 290, 1262, 1317, 2091, 2226, 2650, 3653, 3845
BseDI	CCNNGG	9 cut(s)	153, 437, 740, 1534, 2089, 2225, 2366, 2538, 2616
BseGI	GGATG	7 cut(s)	400, 1801, 1810, 2044, 2658, 2818, 3491
BseJI	GATNNNNATC	1 cut(s)	2817
BseLI	CCNNNNNNNGG	9 cut(s)	159, 531, 578, 1538, 2054, 2226, 2456, 2803, 3557
BseMI	GCAATG	4 cut(s)	675, 1929, 2593, 3004
BseNI	ACTGG	6 cut(s)	1415, 1445, 1477, 1564, 1620, 2254
BseRI	GAGGAG	3 cut(s)	194, 197, 514
BseSI	GKGCMC	2 cut(s)	771, 2373
BsgI	GTGCAG	1 cut(s)	1923
Bsh1236I	CGCG	2 cut(s)	1140, 2148
BshFI	GGCC	7 cut(s)	288, 781, 1491, 2615, 3204, 3651, 3718
BshNI	GGYRCC	1 cut(s)	3694
BsiHKAI	GWGCWC	4 cut(s)	35, 771, 1217, 3679
BsiHKCI	CYCGRG	2 cut(s)	2366, 3615
BsiSI	CCGG	3 cut(s)	245, 2367, 2429
BslFI	GGGAC	3 cut(s)	1394, 1489, 2432
BslI	CCNNNNNNNGG	9 cut(s)	159, 531, 578, 1538, 2054, 2226, 2456, 2803, 3557
BsmAI	GTCTC	3 cut(s)	1913, 2201, 3317
BsmBI	CGTCTC	1 cut(s)	1913
BsmFI	GGGAC	3 cut(s)	1394, 1489, 2432
BsmI	GAATGC	3 cut(s)	1239, 2569, 3832
BsnI	GGCC	7 cut(s)	288, 781, 1491, 2615, 3204, 3651, 3718
Bso31I	GGTCTC	1 cut(s)	2201
BsoBI	CYCGRG	2 cut(s)	2366, 3615
Bsp1286I	GDGCHC	6 cut(s)	35, 692, 771, 1217, 2373, 3679
Bsp13I	TCCGGA	1 cut(s)	244
Bsp1407I	TGTACA	2 cut(s)	2679, 3381
Bsp143I	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
Bsp1720I	GCTNAGC	1 cut(s)	1017
Bsp19I	CCATGG	1 cut(s)	2616
BspACI	CCGC	9 cut(s)	966, 1204, 1412, 2132, 2701, 2738, 3298, 3868, 3923
BspANI	GGCC	7 cut(s)	288, 781, 1491, 2615, 3204, 3651, 3718
BspEI	TCCGGA	1 cut(s)	244
BspFNI	CGCG	2 cut(s)	1140, 2148
BspHI	TCATGA	4 cut(s)	1116, 1198, 1620, 2472
BspLI	GGNNCC	6 cut(s)	689, 912, 2365, 2981, 3256, 3696
BspMAI	CTGCAG	1 cut(s)	2398
BspPI	GGATC	4 cut(s)	453, 816, 1307, 2814
BspQI	GCTCTTC	1 cut(s)	2341
BspT107I	GGYRCC	1 cut(s)	3694
BspTNI	GGTCTC	1 cut(s)	2201
BsrBI	CCGCTC	1 cut(s)	1204
BsrDI	GCAATG	4 cut(s)	675, 1929, 2593, 3004
BsrGI	TGTACA	2 cut(s)	2679, 3381
BsrI	ACTGG	6 cut(s)	1415, 1445, 1477, 1564, 1620, 2254
BssECI	CCNNGG	9 cut(s)	153, 437, 740, 1534, 2089, 2225, 2366, 2538, 2616
BssMI	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
BssNI	GRCGYC	1 cut(s)	3406
BssSI	CACGAG	2 cut(s)	1242, 3678
BssT1I	CCWWGG	4 cut(s)	437, 740, 2538, 2616
Bst2BI	CACGAG	2 cut(s)	1242, 3678
Bst2UI	CCWGG	9 cut(s)	155, 290, 1262, 1317, 2091, 2226, 2650, 3653, 3845
Bst6I	CTCTTC	3 cut(s)	2341, 3315, 3487
BstACI	GRCGYC	1 cut(s)	3406
BstAPI	GCANNNNNTGC	4 cut(s)	1007, 2330, 2741, 3629
BstAUI	TGTACA	2 cut(s)	2679, 3381
BstBAI	YACGTR	2 cut(s)	772, 1598
BstC8I	GCNNGC	5 cut(s)	934, 1142, 2099, 2130, 3310
BstDSI	CCRYGG	2 cut(s)	1534, 2616
BstF5I	GGATG	7 cut(s)	400, 1801, 1810, 2044, 2658, 2818, 3491
BstFNI	CGCG	2 cut(s)	1140, 2148
BstH2I	RGCGCY	1 cut(s)	2829
BstHHI	GCGC	1 cut(s)	2828
BstKTI	GATC	9 cut(s)	448, 753, 811, 1315, 2176, 2809, 3196, 3807, 3951
BstMAI	GTCTC	3 cut(s)	1913, 2201, 3317
BstMBI	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
BstNI	CCWGG	9 cut(s)	155, 290, 1262, 1317, 2091, 2226, 2650, 3653, 3845
BstNSI	RCATGY	5 cut(s)	557, 2105, 3146, 3418, 3901
BstSFI	CTRYAG	6 cut(s)	541, 762, 1308, 2019, 2394, 2943
BstSLI	GKGCMC	2 cut(s)	771, 2373
BstSNI	TACGTA	1 cut(s)	1598
BstUI	CGCG	2 cut(s)	1140, 2148
BstV2I	GAAGAC	1 cut(s)	1380
BstX2I	RGATCY	5 cut(s)	445, 808, 1312, 3193, 3804
BstXI	CCANNNNNNTGG	1 cut(s)	2200
BstYI	RGATCY	5 cut(s)	445, 808, 1312, 3193, 3804
Bsu36I	CCTNAGG	2 cut(s)	226, 3259
BsuI	GTATCC	1 cut(s)	2509
BsuRI	GGCC	7 cut(s)	288, 781, 1491, 2615, 3204, 3651, 3718
BtgI	CCRYGG	2 cut(s)	1534, 2616
BtsCI	GGATG	7 cut(s)	400, 1801, 1810, 2044, 2658, 2818, 3491
BtsI	GCAGTG	3 cut(s)	2966, 3100, 3300
Cac8I	GCNNGC	5 cut(s)	934, 1142, 2099, 2130, 3310
CaiI	CAGNNNCTG	2 cut(s)	2018, 2741
CciI	TCATGA	4 cut(s)	1116, 1198, 1620, 2472
CfoI	GCGC	1 cut(s)	2828
Cfr13I	GGNCC	3 cut(s)	569, 2363, 3560
Cfr9I	CCCGGG	1 cut(s)	2366
Csp6I	GTAC	6 cut(s)	326, 467, 1254, 1981, 2680, 3382
CspCI	CAANNNNNGTGG	2 cut(s)	3573, 3608
CviQI	GTAC	6 cut(s)	326, 467, 1254, 1981, 2680, 3382
DpnI	GATC	9 cut(s)	447, 752, 810, 1314, 2175, 2808, 3195, 3806, 3950
DpnII	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
DriI	GACNNNNNGTC	1 cut(s)	3405
EaeI	YGGCCR	3 cut(s)	286, 1489, 3716
Eam1104I	CTCTTC	3 cut(s)	2341, 3315, 3487
Eam1105I	GACNNNNNGTC	1 cut(s)	3405
EarI	CTCTTC	3 cut(s)	2341, 3315, 3487
Ecl136II	GAGCTC	1 cut(s)	33
Eco105I	TACGTA	1 cut(s)	1598
Eco130I	CCWWGG	4 cut(s)	437, 740, 2538, 2616
Eco24I	GRGCYC	2 cut(s)	35, 692
Eco31I	GGTCTC	1 cut(s)	2201
Eco47I	GGWCC	3 cut(s)	569, 2363, 3560
Eco47III	AGCGCT	1 cut(s)	2827
Eco53kI	GAGCTC	1 cut(s)	33
Eco57I	CTGAAG	3 cut(s)	1130, 3291, 3468
Eco81I	CCTNAGG	2 cut(s)	226, 3259
Eco88I	CYCGRG	2 cut(s)	2366, 3615
EcoICRI	GAGCTC	1 cut(s)	33
EcoO109I	RGGNCCY	1 cut(s)	2363
EcoRII	CCWGG	9 cut(s)	153, 288, 1260, 1315, 2089, 2224, 2648, 3651, 3843
EcoT14I	CCWWGG	4 cut(s)	437, 740, 2538, 2616
EcoT22I	ATGCAT	2 cut(s)	3116, 3420
EcoT38I	GRGCYC	2 cut(s)	35, 692
ErhI	CCWWGG	4 cut(s)	437, 740, 2538, 2616
Esp3I	CGTCTC	1 cut(s)	1913
FalI	AAGNNNNNCTT	2 cut(s)	937, 969
FaqI	GGGAC	3 cut(s)	1394, 1489, 2432
FauI	CCCGC	2 cut(s)	1419, 2708
FauNDI	CATATG	2 cut(s)	1030, 2722
FokI	GGATG	7 cut(s)	407, 1808, 1817, 2031, 2665, 2825, 3478
FriOI	GRGCYC	2 cut(s)	35, 692
GlaI	GCGC	1 cut(s)	2827
GsuI	CTGGAG	3 cut(s)	57, 2632, 3684
HaeII	RGCGCY	1 cut(s)	2829
HaeIII	GGCC	7 cut(s)	288, 781, 1491, 2615, 3204, 3651, 3718
HapII	CCGG	3 cut(s)	245, 2367, 2429
HhaI	GCGC	1 cut(s)	2828
Hin1I	GRCGYC	1 cut(s)	3406
Hin6I	GCGC	1 cut(s)	2826
HinP1I	GCGC	1 cut(s)	2826
HindIII	AAGCTT	4 cut(s)	239, 1340, 1956, 3575
HinfI	GANTC	7 cut(s)	296, 419, 1817, 2635, 2786, 3169, 3817
HpaII	CCGG	3 cut(s)	245, 2367, 2429
HphI	GGTGA	4 cut(s)	490, 709, 728, 2555
Hpy166II	GTNNAC	5 cut(s)	413, 769, 2836, 3382, 3384
Hpy8I	GTNNAC	5 cut(s)	413, 769, 2836, 3382, 3384
Hpy99I	CGWCG	2 cut(s)	996, 2246
HpyCH4IV	ACGT	8 cut(s)	313, 609, 771, 1597, 2338, 2554, 3244, 3406
HpySE526I	ACGT	8 cut(s)	313, 609, 771, 1597, 2338, 2554, 3244, 3406
Hsp92I	GRCGYC	1 cut(s)	3406
HspAI	GCGC	1 cut(s)	2826
Kpn2I	TCCGGA	1 cut(s)	244
Kzo9I	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
LguI	GCTCTTC	1 cut(s)	2341
LmnI	GCTCC	8 cut(s)	687, 742, 760, 1206, 1926, 2661, 3063, 3466
LweI	GCATC	9 cut(s)	211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487
MaeII	ACGT	8 cut(s)	313, 609, 771, 1597, 2338, 2554, 3244, 3406
MalI	GATC	9 cut(s)	447, 752, 810, 1314, 2175, 2808, 3195, 3806, 3950
MbiI	CCGCTC	1 cut(s)	1204
MboI	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	5 cut(s)	445, 808, 1312, 3193, 3804
MhlI	GDGCHC	6 cut(s)	35, 692, 771, 1217, 2373, 3679
MlsI	TGGCCA	2 cut(s)	288, 1491
MluNI	TGGCCA	2 cut(s)	288, 1491
MlyI	GAGTC	4 cut(s)	428, 2644, 2780, 3178
MmeI	TCCRAC	5 cut(s)	156, 402, 782, 2540, 3085
Mox20I	TGGCCA	2 cut(s)	288, 1491
Mph1103I	ATGCAT	2 cut(s)	3116, 3420
MroI	TCCGGA	1 cut(s)	244
MroXI	GAANNNNTTC	1 cut(s)	1237
MscI	TGGCCA	2 cut(s)	288, 1491
MslI	CAYNNNNRTG	1 cut(s)	2198
Msp20I	TGGCCA	2 cut(s)	288, 1491
MspA1I	CMGCKG	3 cut(s)	2330, 2738, 3786
MspI	CCGG	3 cut(s)	245, 2367, 2429
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	3 cut(s)	1239, 2569, 3832
MvaI	CCWGG	9 cut(s)	155, 290, 1262, 1317, 2091, 2226, 2650, 3653, 3845
MvnI	CGCG	2 cut(s)	1140, 2148
NciI	CCSGG	2 cut(s)	2367, 2368
NcoI	CCATGG	1 cut(s)	2616
NdeI	CATATG	2 cut(s)	1030, 2722
NdeII	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
NlaIV	GGNNCC	6 cut(s)	689, 912, 2365, 2981, 3256, 3696
NmuCI	GTSAC	5 cut(s)	697, 947, 979, 1555, 3520
NsiI	ATGCAT	2 cut(s)	3116, 3420
NspI	RCATGY	5 cut(s)	557, 2105, 3146, 3418, 3901
OliI	CACNNNNGTG	1 cut(s)	2198
PaeR7I	CTCGAG	1 cut(s)	3615
PagI	TCATGA	4 cut(s)	1116, 1198, 1620, 2472
PciI	ACATGT	1 cut(s)	3142
PciSI	GCTCTTC	1 cut(s)	2341
PctI	GAATGC	3 cut(s)	1239, 2569, 3832
PdmI	GAANNNNTTC	1 cut(s)	1237
PfeI	GAWTC	3 cut(s)	296, 1817, 3817
PflMI	CCANNNNNTGG	2 cut(s)	2803, 3557
PfoI	TCCNGGA	3 cut(s)	1315, 2648, 3843
PleI	GAGTC	4 cut(s)	427, 2643, 2780, 3177
PpsI	GAGTC	4 cut(s)	427, 2643, 2780, 3177
Ppu21I	YACGTR	2 cut(s)	772, 1598
PpuMI	RGGWCCY	1 cut(s)	2363
PscI	ACATGT	1 cut(s)	3142
PshBI	ATTAAT	2 cut(s)	1704, 2711
Psp124BI	GAGCTC	1 cut(s)	35
Psp1406I	AACGTT	1 cut(s)	2554
Psp5II	RGGWCCY	1 cut(s)	2363
Psp6I	CCWGG	9 cut(s)	153, 288, 1260, 1315, 2089, 2224, 2648, 3651, 3843
PspGI	CCWGG	9 cut(s)	153, 288, 1260, 1315, 2089, 2224, 2648, 3651, 3843
PspN4I	GGNNCC	6 cut(s)	689, 912, 2365, 2981, 3256, 3696
PspPI	GGNCC	3 cut(s)	569, 2363, 3560
PspPPI	RGGWCCY	1 cut(s)	2363
PstI	CTGCAG	1 cut(s)	2398
PstNI	CAGNNNCTG	2 cut(s)	2018, 2741
PsuI	RGATCY	5 cut(s)	445, 808, 1312, 3193, 3804
PvuII	CAGCTG	2 cut(s)	2330, 3786
RsaI	GTAC	6 cut(s)	327, 468, 1255, 1982, 2681, 3383
RsaNI	GTAC	6 cut(s)	326, 467, 1254, 1981, 2680, 3382
RseI	CAYNNNNRTG	1 cut(s)	2198
SacI	GAGCTC	1 cut(s)	35
SapI	GCTCTTC	1 cut(s)	2341
Sau3AI	GATC	9 cut(s)	445, 750, 808, 1312, 2173, 2806, 3193, 3804, 3948
Sau96I	GGNCC	3 cut(s)	569, 2363, 3560
ScaI	AGTACT	1 cut(s)	1255
SchI	GAGTC	4 cut(s)	428, 2644, 2780, 3178
SduI	GDGCHC	6 cut(s)	35, 692, 771, 1217, 2373, 3679
SfaNI	GCATC	9 cut(s)	211, 385, 1651, 1795, 2014, 2333, 2643, 2923, 3487
SfcI	CTRYAG	6 cut(s)	541, 762, 1308, 2019, 2394, 2943
Sfr274I	CTCGAG	1 cut(s)	3615
SinI	GGWCC	3 cut(s)	569, 2363, 3560
SlaI	CTCGAG	1 cut(s)	3615
SmaI	CCCGGG	1 cut(s)	2368
SmiMI	CAYNNNNRTG	1 cut(s)	2198
SmlI	CTYRAG	4 cut(s)	28, 1210, 3615, 3667
SmoI	CTYRAG	4 cut(s)	28, 1210, 3615, 3667
SnaBI	TACGTA	1 cut(s)	1598
SpeI	ACTAGT	2 cut(s)	2179, 3428
SsiI	CCGC	9 cut(s)	966, 1204, 1412, 2132, 2701, 2738, 3298, 3868, 3923
SspI	AATATT	3 cut(s)	1131, 2439, 2488
SstI	GAGCTC	1 cut(s)	35
StyI	CCWWGG	4 cut(s)	437, 740, 2538, 2616
TaiI	ACGT	8 cut(s)	316, 612, 774, 1600, 2341, 2557, 3247, 3409
TaqI	TCGA	4 cut(s)	961, 2176, 2244, 3616
TatI	WGTACW	6 cut(s)	325, 466, 1253, 1980, 2679, 3381
TauI	GCSGC	3 cut(s)	2135, 2741, 3925
TfiI	GAWTC	3 cut(s)	296, 1817, 3817
TseFI	GTSAC	5 cut(s)	697, 947, 979, 1555, 3520
Tsp45I	GTSAC	5 cut(s)	697, 947, 979, 1555, 3520
TspGWI	ACGGA	3 cut(s)	1232, 1493, 3453
TspMI	CCCGGG	1 cut(s)	2366
Van91I	CCANNNNNTGG	2 cut(s)	2803, 3557
VneI	GTGCAC	1 cut(s)	767
VpaK11BI	GGWCC	3 cut(s)	569, 2363, 3560
VspI	ATTAAT	2 cut(s)	1704, 2711
XapI	RAATTY	8 cut(s)	7, 719, 1088, 1729, 2411, 2912, 3748, 3874
XbaI	TCTAGA	1 cut(s)	1631
XceI	RCATGY	5 cut(s)	557, 2105, 3146, 3418, 3901
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1366, 2051
XhoI	CTCGAG	1 cut(s)	3615
XmaI	CCCGGG	1 cut(s)	2366
XmnI	GAANNNNTTC	1 cut(s)	1237
ZraI	GACGTC	1 cut(s)	3407
ZrmI	AGTACT	1 cut(s)	1255
Zsp2I	ATGCAT	2 cut(s)	3116, 3420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.