FvH4_4g00362

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000849
Physical Location & Seq
Reverse (-)
355153 .. 361523
6371 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00362.t1

Sequence Viewer

Length: 807 bp
ATGGATTTAACAGAGTTGGAGAGGTTACCGGATGAATGCATGGCCCATGTTCTTTCCCTCACGTCCCCCAAAGACGCTTGCCGATCAGCCTCCGTCTCCTCCTCCTTTCGATCGGCCGTGCAATCCCAAACTCTTTGGCAACGTTTCTTACCCCATGACTATCGTCAAATCATCTCTCGATCCCTTGCTCCTCCTCTCCCATTCATCTCCTCTCTCTCTAACAAGGACCTCTACTTTCGTCTCTGCGACTCCCCCATCTTCCTCGACGATGGCAATCTGAGTTTCTCATTGGATAAACTGAGTGGTAAAAAATGTTGGATGCTGGGAGCCAAACTGTTGAGCATTACATGGGCAGACACTCCAGAATACTGGGAATGGAATTCCCTACCCGAATCCAGGTTCTCCGAAGTGGCTGAGCTCAGACTTGTATGGTGGCTTGAGATTAAGGGATACATAGACACTAAAATATTGTCCCTGCGAACAACCTATGCAGCTTACCTGGTATATCAGCTCCCAGAAGAATCACAATGGGGGTTTCTGGGAATACCTATGACGGTGCGTGTCACCAATGAACAAAATGAAGTTGTAGCTGAGCATGATGTGTTCCTGGCAGACAAGCCAGAAGATGTGCCAGCTCCACAAGCTCAAAGCAGAGGAGATGGATGGATGGAGATTGAGTTGGGTGAAATTTCCAATCAAGAAGATGATGCTACTGTTGAGTTTTGTCTGAGGGAAACTGAGAGTGGCATCCCTAAGGGAGGCCTCATTGTGGAAGGTATTGAGCTTAGGCCTAAACTAAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

269

Amino Acids

30.48

Weight (kDa)

4.63

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 47 3.3e-09 F-box domain
F-box-like PF12937 6 - 49 3.3e-09 F-box-like
PP2 PF14299 103 - 264 2.7e-48 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 142
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 3 cut(s) 379, 687, 799
AfiI CCNNNNNNNGG 3 cut(s) 396, 758, 769
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 3 cut(s) 395, 498, 606
AluBI AGCT 7 cut(s) 418, 494, 511, 590, 635, 644, 784
AluI AGCT 7 cut(s) 418, 494, 511, 590, 635, 644, 784
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 2 cut(s) 100, 245
AlwI GGATC 1 cut(s) 174
AoxI GGCC 4 cut(s) 42, 114, 760, 788
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 3 cut(s) 379, 687, 799
AspS9I GGNCC 2 cut(s) 43, 226
AsuHPI GGTGA 2 cut(s) 556, 695
AvaII GGWCC 1 cut(s) 226
AxyI CCTNAGG 1 cut(s) 753
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 503
BccI CCATC 5 cut(s) 263, 263, 653, 657, 661
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 3 cut(s) 397, 500, 608
BciVI GTATCC 1 cut(s) 443
BcoDI GTCTC 2 cut(s) 100, 245
BfuI GTATCC 1 cut(s) 443
BisI GCNGC 1 cut(s) 492
BlpI GCTNAGC 2 cut(s) 414, 591
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 3 cut(s) 397, 500, 608
Bme18I GGWCC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 43, 226
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 3 cut(s) 397, 500, 608
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 3 cut(s) 309, 697, 756
BmuI ACTGGG 1 cut(s) 379
BoxI GACNNNNGTC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 785
Bpu1102I GCTNAGC 2 cut(s) 414, 591
BpuEI CTTGAG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 662, 692
Bsc4I CCNNNNNNNGG 3 cut(s) 396, 758, 769
Bse1I ACTGG 1 cut(s) 374
Bse21I CCTNAGG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 3 cut(s) 397, 500, 608
BseGI GGATG 5 cut(s) 37, 324, 668, 672, 747
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 396, 758, 769
BseMII CTCAG 7 cut(s) 269, 290, 405, 433, 582, 719, 729
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 6 cut(s) 88, 91, 180, 183, 199, 669
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 503
BseYI CCCAGC 1 cut(s) 322
Bsh1285I CGRYCG 2 cut(s) 113, 117
BshFI GGCC 4 cut(s) 44, 116, 762, 790
BsiEI CGRYCG 2 cut(s) 113, 117
BsiHKAI GWGCWC 1 cut(s) 420
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 49, 457
BslI CCNNNNNNNGG 3 cut(s) 396, 758, 769
BsmAI GTCTC 2 cut(s) 100, 245
BsmBI CGTCTC 2 cut(s) 100, 245
BsmFI GGGAC 2 cut(s) 49, 457
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 4 cut(s) 44, 116, 762, 790
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 3 cut(s) 83, 110, 179
Bsp1720I GCTNAGC 2 cut(s) 414, 591
BspANI GGCC 4 cut(s) 44, 116, 762, 790
BspCNI CTCAG 7 cut(s) 270, 291, 406, 432, 583, 720, 730
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 1 cut(s) 174
BsrI ACTGG 1 cut(s) 374
BssMI GATC 3 cut(s) 83, 110, 179
Bst2UI CCWGG 3 cut(s) 397, 500, 608
Bst4CI ACNGT 3 cut(s) 336, 556, 715
BstC8I GCNNGC 2 cut(s) 79, 633
BstDEI CTNAG 9 cut(s) 278, 299, 414, 419, 591, 728, 738, 753, 785
BstEII GGTNACC 1 cut(s) 24
BstENI CCTNNNNNAGG 1 cut(s) 756
BstF5I GGATG 5 cut(s) 37, 324, 668, 672, 747
BstKTI GATC 3 cut(s) 86, 113, 182
BstMAI GTCTC 2 cut(s) 100, 245
BstMBI GATC 3 cut(s) 83, 110, 179
BstMCI CGRYCG 2 cut(s) 113, 117
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNI CCWGG 3 cut(s) 397, 500, 608
BstPAI GACNNNNGTC 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 24
BstSCI CCNGG 3 cut(s) 395, 498, 606
BstV1I GCAGC 1 cut(s) 503
BstXI CCANNNNNNTGG 1 cut(s) 369
BstZI CGGCCG 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 753
BsuI GTATCC 1 cut(s) 443
BsuRI GGCC 4 cut(s) 44, 116, 762, 790
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 5 cut(s) 37, 324, 668, 672, 747
Cac8I GCNNGC 2 cut(s) 79, 633
Cfr13I GGNCC 2 cut(s) 43, 226
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 498
CviAII CATG 5 cut(s) 40, 47, 155, 348, 596
DdeI CTNAG 9 cut(s) 278, 299, 414, 419, 591, 728, 738, 753, 785
DpnI GATC 3 cut(s) 85, 112, 181
DpnII GATC 3 cut(s) 83, 110, 179
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 114
Eco147I AGGCCT 2 cut(s) 762, 790
Eco24I GRGCYC 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 226
Eco52I CGGCCG 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 418
Eco81I CCTNAGG 1 cut(s) 753
Eco91I GGTNACC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 3 cut(s) 395, 498, 606
EcoT22I ATGCAT 1 cut(s) 41
EcoT38I GRGCYC 1 cut(s) 420
Esp3I CGTCTC 2 cut(s) 100, 245
FaeI CATG 5 cut(s) 43, 50, 158, 351, 599
FaqI GGGAC 2 cut(s) 49, 457
FatI CATG 5 cut(s) 39, 46, 154, 347, 595
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 5 cut(s) 44, 331, 675, 679, 734
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 492
GluI GCNGC 1 cut(s) 492
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 4 cut(s) 44, 116, 762, 790
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 83
Hin1II CATG 5 cut(s) 43, 50, 158, 351, 599
HinfI GANTC 3 cut(s) 248, 392, 521
HpaII CCGG 1 cut(s) 29
HphI GGTGA 2 cut(s) 556, 695
Hpy188I TCNGA 4 cut(s) 279, 406, 422, 729
Hpy188III TCNNGA 3 cut(s) 177, 362, 698
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 767
HpyCH4III ACNGT 3 cut(s) 336, 556, 715
HpyCH4IV ACGT 2 cut(s) 62, 142
HpyCH4V TGCA 3 cut(s) 39, 121, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 9 cut(s) 278, 299, 414, 419, 591, 728, 738, 753, 785
HpySE526I ACGT 2 cut(s) 62, 142
Hsp92II CATG 5 cut(s) 43, 50, 158, 351, 599
Kzo9I GATC 3 cut(s) 83, 110, 179
LmnI GCTCC 4 cut(s) 193, 326, 516, 640
Lsp1109I GCAGC 1 cut(s) 503
LweI GCATC 3 cut(s) 309, 697, 756
MabI ACCWGGT 1 cut(s) 498
MaeII ACGT 2 cut(s) 62, 142
MaeIII GTNAC 2 cut(s) 24, 562
MalI GATC 3 cut(s) 85, 112, 181
MboI GATC 3 cut(s) 83, 110, 179
MboII GAAGA 4 cut(s) 250, 530, 635, 713
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 3 cut(s) 379, 687, 799
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 41
MseI TTAA 2 cut(s) 8, 444
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 3 cut(s) 397, 500, 608
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 3 cut(s) 397, 500, 608
MwoI GCNNNNNNNGC 1 cut(s) 641
NdeII GATC 3 cut(s) 83, 110, 179
NlaIII CATG 5 cut(s) 43, 50, 158, 351, 599
NlaIV GGNNCC 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 562
NsiI ATGCAT 1 cut(s) 41
PceI AGGCCT 2 cut(s) 762, 790
PctI GAATGC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 392, 521
PkrI GCNGC 1 cut(s) 493
Ple19I CGATCG 1 cut(s) 113
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PpuMI RGGWCCY 1 cut(s) 226
PshAI GACNNNNGTC 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 420
Psp1406I AACGTT 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 3 cut(s) 395, 498, 606
PspEI GGTNACC 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 3 cut(s) 395, 498, 606
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 43, 226
PspPPI RGGWCCY 1 cut(s) 226
PvuI CGATCG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 2 cut(s) 8, 444
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 3 cut(s) 83, 110, 179
Sau96I GGNCC 2 cut(s) 43, 226
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 3 cut(s) 397, 500, 608
SduI GDGCHC 1 cut(s) 420
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 3 cut(s) 309, 697, 756
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 3 cut(s) 379, 687, 799
SseBI AGGCCT 2 cut(s) 762, 790
SspI AATATT 1 cut(s) 468
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 2 cut(s) 762, 790
StyD4I CCNGG 3 cut(s) 395, 498, 606
TaaI ACNGT 3 cut(s) 336, 556, 715
TaiI ACGT 2 cut(s) 65, 145
TaqI TCGA 3 cut(s) 109, 178, 264
TasI AATT 3 cut(s) 379, 687, 799
TfiI GAWTC 2 cut(s) 392, 521
Tru1I TTAA 2 cut(s) 8, 444
Tru9I TTAA 2 cut(s) 8, 444
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 4 cut(s) 48, 193, 585, 594
TspGWI ACGGA 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 226
XagI CCTNNNNNAGG 1 cut(s) 756
XapI RAATTY 3 cut(s) 379, 687, 799
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.