Rh4DG003700

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000849
Physical Location & Seq
Reverse (-)
603250 .. 605029
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG003700.1

Sequence Viewer

Length: 555 bp
ATGGTGGTGAATTTAACACAGAGCTTTGCACTAGAAGACAAGAGTGGAAAGAAATGTTATATGATAGGGGCAAGAGGGCTTTCAATTGCAGGGGAAGAAGACAAACTCGGTTATTGGCAATGGACAACCGTACCAGCATCTAGGTTTTGTGAAGTGGCTGAGCTGAAAAATGTATTGGGGCTTGACATCAAGGCACATATCAAAACTAGAATGTTGTCTCCGGGTACAACCTATGCGGCTTACTTTGTCTATCAGCTCTTAGGATGCAGATTTGGGTTTGCACAAACGCCAATTGAGTTTCGTGTCACTTGGGAACAAAGCCGAGTAGAAGCGTCTTACCATGGGGTATGCCTAGAACGTGTGGTGCTAGACCCGTCCGCAGCGTCTCCACGAGCTCAATATAGAGGAGATGGGTGGATGGAGGTGGAGATGGGTGAGTTCTTCATCAAAGAAGGAGATAATGCTACTGTGGAGTGTAGTGTGAGGGAAGTTAATGGCACTAAGAGCGGCCTCCTTGTGGAAGGCATTGAGATTAGGCCTAAACTAGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

184

Amino Acids

20.59

Weight (kDa)

5.53

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 17 - 180 2e-43 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 507
AciI CCGC 3 cut(s) 236, 378, 507
AcsI RAATTY 1 cut(s) 10
AfaI GTAC 2 cut(s) 132, 226
AfiI CCNNNNNNNGG 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 4 cut(s) 24, 163, 256, 395
AluI AGCT 4 cut(s) 24, 163, 256, 395
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 2 cut(s) 222, 390
AoxI GGCC 2 cut(s) 508, 536
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 19, 446
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BanII GRGCYC 1 cut(s) 397
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 2 cut(s) 42, 105
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 1 cut(s) 392
BccI CCATC 3 cut(s) 404, 412, 424
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 222, 390
BfaI CTAG 6 cut(s) 32, 141, 207, 353, 368, 545
BisI GCNGC 3 cut(s) 237, 381, 508
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 3 cut(s) 238, 382, 509
Bme1390I CCNGG 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 146, 254
BpiI GAAGAC 2 cut(s) 42, 105
Bpu1102I GCTNAGC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 2 cut(s) 269, 423
BseLI CCNNNNNNNGG 1 cut(s) 517
BseMI GCAATG 1 cut(s) 125
BseMII CTCAG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 392
BshFI GGCC 2 cut(s) 510, 538
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 2 cut(s) 222, 390
BsmBI CGTCTC 1 cut(s) 390
BsnI GGCC 2 cut(s) 510, 538
Bsp1286I GDGCHC 1 cut(s) 397
Bsp1720I GCTNAGC 1 cut(s) 159
Bsp19I CCATGG 1 cut(s) 340
BspACI CCGC 3 cut(s) 236, 378, 507
BspANI GGCC 2 cut(s) 510, 538
BspCNI CTCAG 1 cut(s) 151
BsrBI CCGCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 340
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 130, 469
BstDEI CTNAG 3 cut(s) 159, 259, 501
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 2 cut(s) 269, 423
BstMAI GTCTC 2 cut(s) 222, 390
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstSCI CCNGG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 2 cut(s) 42, 105
BsuRI GGCC 2 cut(s) 510, 538
BtgI CCRYGG 1 cut(s) 340
BtsCI GGATG 2 cut(s) 269, 423
CseI GACGC 2 cut(s) 321, 372
Csp6I GTAC 2 cut(s) 131, 225
CviAII CATG 1 cut(s) 341
CviQI GTAC 2 cut(s) 131, 225
DdeI CTNAG 3 cut(s) 159, 259, 501
Ecl136II GAGCTC 1 cut(s) 395
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 1 cut(s) 344
FaiI YATR 8 cut(s) 60, 62, 198, 234, 342, 349, 402, 551
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 3 cut(s) 237, 381, 508
FokI GGATG 2 cut(s) 276, 430
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 3 cut(s) 237, 381, 508
FspBI CTAG 6 cut(s) 32, 141, 207, 353, 368, 545
GluI GCNGC 3 cut(s) 237, 381, 508
HaeIII GGCC 2 cut(s) 510, 538
HapII CCGG 1 cut(s) 221
HgaI GACGC 2 cut(s) 321, 372
Hin1II CATG 1 cut(s) 344
HpaII CCGG 1 cut(s) 221
HphI GGTGA 2 cut(s) 19, 446
HpyAV CCTTC 2 cut(s) 446, 515
HpyCH4III ACNGT 2 cut(s) 130, 469
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 4 cut(s) 29, 89, 267, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 3 cut(s) 159, 259, 501
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 1 cut(s) 344
LpnPI CCDG 3 cut(s) 75, 147, 234
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 2 cut(s) 146, 254
MaeI CTAG 6 cut(s) 32, 141, 207, 353, 368, 545
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 304
MbiI CCGCTC 1 cut(s) 507
MboII GAAGA 4 cut(s) 47, 107, 110, 433
MfeI CAATTG 2 cut(s) 84, 291
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 3 cut(s) 10, 84, 291
MnlI CCTC 5 cut(s) 68, 398, 415, 477, 521
MseI TTAA 2 cut(s) 14, 492
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 2 cut(s) 84, 291
MwoI GCNNNNNNNGC 1 cut(s) 504
NciI CCSGG 1 cut(s) 222
NcoI CCATGG 1 cut(s) 340
NlaIII CATG 1 cut(s) 344
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 304
PceI AGGCCT 1 cut(s) 538
PkrI GCNGC 3 cut(s) 238, 382, 509
Psp124BI GAGCTC 1 cut(s) 397
RsaI GTAC 2 cut(s) 132, 226
RsaNI GTAC 2 cut(s) 131, 225
SacI GAGCTC 1 cut(s) 397
SaqAI TTAA 2 cut(s) 14, 492
SatI GCNGC 3 cut(s) 237, 381, 508
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 397
SetI ASST 8 cut(s) 26, 146, 165, 233, 258, 361, 397, 426
SfaNI GCATC 2 cut(s) 146, 254
Sse9I AATT 3 cut(s) 10, 84, 291
SseBI AGGCCT 1 cut(s) 538
SsiI CCGC 3 cut(s) 236, 378, 507
SspMI CTAG 6 cut(s) 32, 141, 207, 353, 368, 545
SstI GAGCTC 1 cut(s) 397
StuI AGGCCT 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 130, 469
TaiI ACGT 1 cut(s) 361
TasI AATT 3 cut(s) 10, 84, 291
TauI GCSGC 2 cut(s) 239, 510
Tru1I TTAA 2 cut(s) 14, 492
Tru9I TTAA 2 cut(s) 14, 492
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 433
XapI RAATTY 1 cut(s) 10
XspI CTAG 6 cut(s) 32, 141, 207, 353, 368, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.