Rh4AG004000

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000849
Physical Location & Seq
Reverse (-)
624931 .. 625584
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG004000.1

Sequence Viewer

Length: 495 bp
ATGATAGGGGCAAGAGGGCTTTCAATTGCAGGGGAAGAAGACAAACTCGGTTATTGGCAATGGACAACCGTACCAGCATCTAGGTTTTGTGAAGTGGCTGAGTTGAAAAATGTATTGGGGCTTGAGATCAAGGCACACATCAAAACTAGAATGTTGTCTCCGGGAACAACCTACGCGGCTTACTTTGTCTATCAGCTCTTAGGATGCAGATTTGGGTTTGCACAAACGCCAATTGAGTTTCGCCTTACTTGGGAACAAAGCCAAGTAGAAGCGTCTTACCATGGGGTATGCCTAGAACGTGTGGTTCTAGACCCGTCCGCAGCGTCTCCACGAGCTCAATATAGAAGAGATGGGTGGATGGAGGTGGAGATGGGTGAGTTCTTCATCAAAGAAGGAGATAATGCTACTGTGGAGTGTAGTGTGAGGGAAGTTAATGGCACTAAGAGCGGCCTCCTTGTGGAAGGCATTGAGATTAGGCCTAAACTAGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

164

Amino Acids

18.45

Weight (kDa)

5.3

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 1 - 160 1.8e-40 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 176, 318, 447
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 250, 457
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 2 cut(s) 24, 106
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 2 cut(s) 196, 335
AluI AGCT 2 cut(s) 196, 335
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 162, 330
AoxI GGCC 2 cut(s) 448, 476
ApeKI GCWGC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 386
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 1 cut(s) 337
BauI CACGAG 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 332
BccI CCATC 3 cut(s) 344, 352, 364
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 162, 330
BfaI CTAG 5 cut(s) 81, 147, 293, 308, 485
BisI GCNGC 3 cut(s) 177, 321, 448
BlsI GCNGC 3 cut(s) 178, 322, 449
Bme1390I CCNGG 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 86, 194
BpiI GAAGAC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 280
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 457
Bse3DI GCAATG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 209, 363
BseLI CCNNNNNNNGG 2 cut(s) 250, 457
BseMI GCAATG 1 cut(s) 65
BseMII CTCAG 1 cut(s) 90
BseXI GCAGC 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 450, 478
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 250, 457
BsmAI GTCTC 2 cut(s) 162, 330
BsmBI CGTCTC 1 cut(s) 330
BsnI GGCC 2 cut(s) 450, 478
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 3 cut(s) 176, 318, 447
BspANI GGCC 2 cut(s) 450, 478
BspCNI CTCAG 1 cut(s) 91
BspFNI CGCG 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 70, 409
Bst6I CTCTTC 1 cut(s) 340
BstDEI CTNAG 3 cut(s) 99, 199, 441
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 209, 363
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 162, 330
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 160
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 450, 478
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 209, 363
CseI GACGC 2 cut(s) 261, 312
Csp6I GTAC 1 cut(s) 71
CviAII CATG 1 cut(s) 281
CviJI RGCY 9 cut(s) 19, 98, 121, 179, 196, 261, 335, 450, 478
CviKI_1 RGCY 9 cut(s) 19, 98, 121, 179, 196, 261, 335, 450, 478
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 3 cut(s) 99, 199, 441
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 280
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 1 cut(s) 284
FaiI YATR 4 cut(s) 282, 289, 342, 491
FatI CATG 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 177, 321, 448
FokI GGATG 2 cut(s) 216, 370
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 3 cut(s) 177, 321, 448
FspBI CTAG 5 cut(s) 81, 147, 293, 308, 485
GluI GCNGC 3 cut(s) 177, 321, 448
HaeIII GGCC 2 cut(s) 450, 478
HapII CCGG 1 cut(s) 161
HgaI GACGC 2 cut(s) 261, 312
Hin1II CATG 1 cut(s) 284
HpaII CCGG 1 cut(s) 161
HphI GGTGA 1 cut(s) 386
Hpy188III TCNNGA 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 386, 455
HpyCH4III ACNGT 2 cut(s) 70, 409
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 29, 207, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 444
HpyF3I CTNAG 3 cut(s) 99, 199, 441
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 1 cut(s) 284
Kzo9I GATC 1 cut(s) 126
LpnPI CCDG 3 cut(s) 15, 87, 174
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 2 cut(s) 86, 194
MaeI CTAG 5 cut(s) 81, 147, 293, 308, 485
MaeII ACGT 1 cut(s) 298
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 447
MboI GATC 1 cut(s) 126
MboII GAAGA 4 cut(s) 47, 50, 357, 373
MfeI CAATTG 2 cut(s) 24, 231
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 2 cut(s) 24, 231
MnlI CCTC 4 cut(s) 8, 355, 417, 461
MseI TTAA 1 cut(s) 432
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 162
MunI CAATTG 2 cut(s) 24, 231
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 444
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 280
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 284
PceI AGGCCT 1 cut(s) 478
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 3 cut(s) 178, 322, 449
Psp124BI GAGCTC 1 cut(s) 337
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 177, 321, 448
Sau3AI GATC 1 cut(s) 126
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 337
SetI ASST 6 cut(s) 86, 173, 198, 301, 337, 366
SfaNI GCATC 2 cut(s) 86, 194
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 24, 231
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 3 cut(s) 176, 318, 447
SspMI CTAG 5 cut(s) 81, 147, 293, 308, 485
SstI GAGCTC 1 cut(s) 337
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 70, 409
TaiI ACGT 1 cut(s) 301
TasI AATT 2 cut(s) 24, 231
TauI GCSGC 2 cut(s) 179, 450
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 1 cut(s) 373
XbaI TCTAGA 1 cut(s) 307
XspI CTAG 5 cut(s) 81, 147, 293, 308, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.