Rw4G000500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000849
Physical Location & Seq
Forward (+)
1131587 .. 1133100
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000500.1

Sequence Viewer

Length: 660 bp
ATGAAAAGGTTGCCCGTGGAGTGCGTCTCCCACATCATTTCCTTTACATCACCTCGCGATGCCTGCAGATCATCAGCTGTCTGTTCTCTGTTTAGAATAGCTGTGGATTCTGATATTGTCTGGAAGAGGCTTCTTCCTCCTAAGCAAATCATTTCGGAATCTGCATCATCGTCCTCGCACAACTGCACAAGAGAGCTTGTAATTGCATGGCCAGATACACCCACTTATTGGCGATGGATGACTCTTCCACACTCCCGTATATATATTTCCTCAAGGGGATGGATGATTATTCTCAAACTTGTATGGTGGCTTGACATCAAGGCGTGCATATCTGCTAAGAACTTATCCCCGGGAACAACCTATTCAACTTACCTTGTGTATCAGCTCTCAGAAGTAAGATCGGGGTTGGCAAGAACACCTATTGTGTTGCGCATCAATTATCGACAAAGTGAAGTTGTGAGTGTGCATAGTGTGATGCTAGACCCTATGCTTCAAGAAGCTCGACACGGAGGAGATGGGTGGATGGAGATTGAGATGGGTCAATTCTTCATTGAACAAGGAAATGATGATACTGCTGTAGAGTGTAGTGTAACGGAAGTTAGTAACCTAAAGGGCGGCCTCATTGTGGAAGGTATTGAGCTTAGGCCTATGCATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

219

Amino Acids

24.86

Weight (kDa)

7.0

Isoelectric Point (pI)

67.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 2 - 44 4.6e-09 F-box-like
F-box PF00646 3 - 42 2.5e-07 F-box domain
PP2 PF14299 63 - 216 6.3e-34 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 431
AccB7I CCANNNNNTGG 1 cut(s) 228
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 615
AcoI YGGCCR 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 228, 625
AgsI TTSAA 3 cut(s) 366, 494, 554
AluBI AGCT 6 cut(s) 77, 101, 196, 385, 500, 640
AluI AGCT 6 cut(s) 77, 101, 196, 385, 500, 640
Alw26I GTCTC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 349
AoxI GGCC 3 cut(s) 209, 616, 644
AspLEI GCGC 1 cut(s) 432
AsuC2I CCSGG 2 cut(s) 350, 351
AsuHPI GGTGA 1 cut(s) 42
AvaI CYCGRG 1 cut(s) 349
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BalI TGGCCA 1 cut(s) 211
BccI CCATC 5 cut(s) 228, 273, 509, 517, 529
BcnI CCSGG 2 cut(s) 350, 351
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 64, 576
BisI GCNGC 1 cut(s) 616
BlsI GCNGC 1 cut(s) 617
Bme1390I CCNGG 2 cut(s) 350, 351
BmeT110I CYCGRG 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 350, 351
BmsI GCATC 4 cut(s) 49, 173, 441, 465
BplI GAGNNNNNCTC 2 cut(s) 11, 43
Bpu10I CCTNAGC 2 cut(s) 141, 641
BpuEI CTTGAG 1 cut(s) 256
BpuMI CCSGG 2 cut(s) 350, 351
BsaJI CCNNGG 3 cut(s) 15, 348, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 625
BseDI CCNNGG 3 cut(s) 15, 348, 349
BseGI GGATG 4 cut(s) 243, 284, 288, 528
BseLI CCNNNNNNNGG 2 cut(s) 228, 625
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 525
BsgI GTGCAG 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 3 cut(s) 211, 618, 646
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 228, 625
BsmAI GTCTC 1 cut(s) 31
BsmBI CGTCTC 1 cut(s) 31
BsnI GGCC 3 cut(s) 211, 618, 646
BsoBI CYCGRG 1 cut(s) 349
Bsp143I GATC 2 cut(s) 68, 398
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 1 cut(s) 615
BspANI GGCC 3 cut(s) 211, 618, 646
BspCNI CTCAG 1 cut(s) 401
BspFNI CGCG 1 cut(s) 57
BspMAI CTGCAG 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 15, 348, 349
BssMI GATC 2 cut(s) 68, 398
Bst6I CTCTTC 2 cut(s) 119, 249
BstC8I GCNNGC 2 cut(s) 64, 325
BstDEI CTNAG 4 cut(s) 141, 336, 388, 641
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 243, 284, 288, 528
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 2 cut(s) 71, 401
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 2 cut(s) 68, 398
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstSCI CCNGG 2 cut(s) 348, 349
BstSFI CTRYAG 2 cut(s) 64, 576
BstUI CGCG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 211, 618, 646
BtgI CCRYGG 1 cut(s) 15
BtgZI GCGATG 2 cut(s) 72, 247
BtsCI GGATG 4 cut(s) 243, 284, 288, 528
BtuMI TCGCGA 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 64, 325
CfoI GCGC 1 cut(s) 432
Cfr9I CCCGGG 1 cut(s) 349
CseI GACGC 1 cut(s) 13
CviAII CATG 1 cut(s) 207
DdeI CTNAG 4 cut(s) 141, 336, 388, 641
DpnI GATC 2 cut(s) 70, 400
DpnII GATC 2 cut(s) 68, 398
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 119, 249
EarI CTCTTC 2 cut(s) 119, 249
Eco147I AGGCCT 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 654
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 1 cut(s) 210
FatI CATG 1 cut(s) 206
FauNDI CATATG 1 cut(s) 654
Fnu4HI GCNGC 1 cut(s) 616
FokI GGATG 4 cut(s) 250, 291, 295, 535
Fsp4HI GCNGC 1 cut(s) 616
FspBI CTAG 1 cut(s) 479
FspI TGCGCA 1 cut(s) 431
GlaI GCGC 1 cut(s) 431
GluI GCNGC 1 cut(s) 616
HaeIII GGCC 3 cut(s) 211, 618, 646
HapII CCGG 1 cut(s) 350
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 432
Hin1II CATG 1 cut(s) 210
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HinfI GANTC 3 cut(s) 107, 158, 241
HpaII CCGG 1 cut(s) 350
HphI GGTGA 1 cut(s) 42
Hpy188I TCNGA 3 cut(s) 112, 157, 391
Hpy188III TCNNGA 3 cut(s) 56, 121, 494
HpyAV CCTTC 1 cut(s) 623
HpyCH4V TGCA 7 cut(s) 66, 164, 186, 206, 327, 466, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 4 cut(s) 141, 336, 388, 641
Hsp92II CATG 1 cut(s) 210
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 2 cut(s) 68, 398
LpnPI CCDG 4 cut(s) 76, 106, 225, 363
LweI GCATC 4 cut(s) 49, 173, 441, 465
MaeI CTAG 1 cut(s) 479
MaeIII GTNAC 2 cut(s) 589, 602
MalI GATC 2 cut(s) 70, 400
MboI GATC 2 cut(s) 68, 398
MboII GAAGA 4 cut(s) 125, 136, 236, 538
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 3 cut(s) 201, 436, 542
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 7 cut(s) 63, 120, 147, 184, 280, 503, 629
Mox20I TGGCCA 1 cut(s) 211
Mph1103I ATGCAT 1 cut(s) 654
MscI TGGCCA 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 211
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 350, 351
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 350, 351
NdeI CATATG 1 cut(s) 654
NdeII GATC 2 cut(s) 68, 398
NlaIII CATG 1 cut(s) 210
NruI TCGCGA 1 cut(s) 57
NsbI TGCGCA 1 cut(s) 431
NsiI ATGCAT 1 cut(s) 654
PceI AGGCCT 1 cut(s) 646
PfeI GAWTC 2 cut(s) 107, 158
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 1 cut(s) 617
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PstI CTGCAG 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 77
RruI TCGCGA 1 cut(s) 57
SatI GCNGC 1 cut(s) 616
Sau3AI GATC 2 cut(s) 68, 398
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 350, 351
SfaNI GCATC 4 cut(s) 49, 173, 441, 465
SfcI CTRYAG 2 cut(s) 64, 576
SmaI CCCGGG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 3 cut(s) 201, 436, 542
SseBI AGGCCT 1 cut(s) 646
SsiI CCGC 1 cut(s) 615
SspMI CTAG 1 cut(s) 479
StuI AGGCCT 1 cut(s) 646
StyD4I CCNGG 2 cut(s) 348, 349
TaqI TCGA 2 cut(s) 442, 502
TasI AATT 3 cut(s) 201, 436, 542
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 2 cut(s) 107, 158
TspDTI ATGAA 2 cut(s) 17, 538
TspGWI ACGGA 2 cut(s) 522, 608
TspMI CCCGGG 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 228
XmaI CCCGGG 1 cut(s) 349
XspI CTAG 1 cut(s) 479
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.