Rorug03G0300100

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000849
Physical Location & Seq
Reverse (-)
31416107 .. 31417611
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0300100.1

Sequence Viewer

Length: 1149 bp
ATGTTAGCAGAACAGGCACGGAGAATCATCACTAACCCTTCTTCACTGATAGCTTGTATATATGAGGCTAAGTATTATCGTTCTGGTACGTTTTGGACAGTAGAACTACATGCTTCTCCATCTTATTCCTGGAGGAGCATTTTTTCTACAAGGGAATTGATTTCTCAAGGCTTTTACTGGCAGATAGGGACTGGTGTGCAAGTGAGGGCGTGGTTGGATCCCTGGGTTCTAGGTATTCCTAATCATACATCGGCGGCACTTGTTCCTGTGGTGAATACTTCTATTAAGGTTAGTGATCTTCTTCTTGATCCTTCTACTTGGAATGAAGTTAAGGTTAGAGAATTATTTTCTCTTGAAGAAGTTGATGCTATATTAAGAATCCCTTTAGGTACTAGAGTGGTAGAAGATCGGTGTGTGTGGAGATTAGAAAAAGATGGGAGCTTTTCTGTCAAAACTGCTTATAGACAATCTTTTGTGAACTCTCCTTCTAGAAGGCCTAGTACTCTTAATGTGTCGAGTGACTTTTGGAAGAAGGTGTGGAAGGGAATGATTCCCACTTCTGCTAAGATGCATATTTGGAGGAAGCAGGCCTTTGAGGACCTTATGTTTTTGTTATGGAGTATCTGGAAAGAGAGAAATTCCAGGGTATGGGAACAAAAGTTGTCACAAGCCTATGATGTTGCTTTACAAGCTTGTACAAGGCTAGCAGAATTTCGAGTTCACAATGGGAAAGCAGGGTGTATTCGTCAGAGAGCTAGTGTGGAGAAGTGGCTGGAGGGGCTTGTCCTCTTCGAAATTTGCAGTCAGCTGAACACGCAGAAGTGTTGGCATGCCAAGCTACAGTCAGGTTTGTACATACTACAACAAAATATGATGCCTGTGCTGCTTGAAACAGATTCAGTGTTGGTGTTGCAGCAGGTTTCTGCTCAAGATCATTCGAATACCTCTGAGTTGGGGAGGTTATATGATGATATTAGAGATATGTTGATGTTACATCCAAATGTGAGGATCAGTCATACAAGGAGGGGTGCCAATGGAGTTGCTCATTCCCTTGCAGACCATGCTAAGGCTCTTCAGCAAGAGACATTTTACTTTTCTGTACCTACTTTTCTCTTAGCTGCTCTTGTAGCTGACTCTATTGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

382

Amino Acids

43.78

Weight (kDa)

9.06

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 286 - 355 4.8e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 907
AccB1I GGYRCC 1 cut(s) 1028
AccB7I CCANNNNNTGG 1 cut(s) 648
AciI CCGC 1 cut(s) 254
AclWI GGATC 4 cut(s) 212, 225, 302, 1016
AcsI RAATTY 3 cut(s) 637, 710, 795
AcuI CTGAAG 1 cut(s) 1058
AfaI GTAC 6 cut(s) 88, 391, 502, 697, 854, 1101
AfiI CCNNNNNNNGG 2 cut(s) 648, 1066
AgsI TTSAA 2 cut(s) 356, 890
AjnI CCWGG 3 cut(s) 128, 221, 641
AluBI AGCT 8 cut(s) 53, 441, 692, 755, 808, 838, 1118, 1130
AluI AGCT 8 cut(s) 53, 441, 692, 755, 808, 838, 1118, 1130
Alw26I GTCTC 1 cut(s) 1076
AlwI GGATC 4 cut(s) 212, 225, 302, 1016
AoxI GGCC 2 cut(s) 494, 588
ApeKI GCWGC 3 cut(s) 883, 913, 1118
ApoI RAATTY 3 cut(s) 637, 710, 795
AspS9I GGNCC 1 cut(s) 598
AsuHPI GGTGA 1 cut(s) 283
AsuII TTCGAA 2 cut(s) 792, 938
AsuNHI GCTAGC 1 cut(s) 703
AvaII GGWCC 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 1028
BarI GAAGNNNNNNTAC 2 cut(s) 484, 516
BbvI GCAGC 3 cut(s) 870, 925, 1105
BccI CCATC 2 cut(s) 127, 428
BciT130I CCWGG 3 cut(s) 130, 223, 643
BcoDI GTCTC 1 cut(s) 1076
BfaI CTAG 6 cut(s) 230, 393, 489, 498, 704, 756
BfmI CTRYAG 1 cut(s) 839
BfuAI ACCTGC 1 cut(s) 907
BisI GCNGC 4 cut(s) 255, 884, 914, 1119
BlsI GCNGC 4 cut(s) 256, 885, 915, 1120
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 3 cut(s) 130, 223, 643
Bme18I GGWCC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 2 cut(s) 219, 1030
BmrFI CCNGG 3 cut(s) 130, 223, 643
BmsI GCATC 3 cut(s) 355, 558, 864
BmtI GCTAGC 1 cut(s) 707
BpmI CTGGAG 2 cut(s) 151, 794
Bpu10I CCTNAGC 1 cut(s) 1065
Bpu14I TTCGAA 2 cut(s) 792, 938
BpuEI CTTGAG 2 cut(s) 150, 912
BsaJI CCNNGG 3 cut(s) 221, 222, 642
Bsc4I CCNNNNNNNGG 2 cut(s) 648, 1066
Bse1I ACTGG 2 cut(s) 182, 196
BseBI CCWGG 3 cut(s) 130, 223, 643
BseDI CCNNGG 3 cut(s) 221, 222, 642
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 2 cut(s) 648, 1066
BseMII CTCAG 1 cut(s) 939
BseNI ACTGG 2 cut(s) 182, 196
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 3 cut(s) 870, 925, 1105
BshFI GGCC 2 cut(s) 496, 590
BshNI GGYRCC 1 cut(s) 1028
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 648, 1066
BsmAI GTCTC 1 cut(s) 1076
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 2 cut(s) 496, 590
Bsp119I TTCGAA 2 cut(s) 792, 938
Bsp1407I TGTACA 2 cut(s) 695, 852
Bsp143I GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
BspACI CCGC 1 cut(s) 254
BspANI GGCC 2 cut(s) 496, 590
BspCNI CTCAG 1 cut(s) 940
BspLI GGNNCC 2 cut(s) 219, 1030
BspMI ACCTGC 1 cut(s) 907
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 4 cut(s) 212, 225, 302, 1016
BspQI GCTCTTC 1 cut(s) 1077
BspT104I TTCGAA 2 cut(s) 792, 938
BspT107I GGYRCC 1 cut(s) 1028
BsrGI TGTACA 2 cut(s) 695, 852
BsrI ACTGG 2 cut(s) 182, 196
BssECI CCNNGG 3 cut(s) 221, 222, 642
BssMI GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
Bst2UI CCWGG 3 cut(s) 130, 223, 643
Bst4CI ACNGT 2 cut(s) 100, 843
Bst6I CTCTTC 2 cut(s) 794, 1077
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstAUI TGTACA 2 cut(s) 695, 852
BstBI TTCGAA 2 cut(s) 792, 938
BstC8I GCNNGC 3 cut(s) 588, 705, 831
BstDEI CTNAG 5 cut(s) 69, 564, 948, 1065, 1114
BstF5I GGATG 1 cut(s) 994
BstKTI GATC 6 cut(s) 220, 298, 310, 409, 934, 1011
BstMAI GTCTC 1 cut(s) 1076
BstMBI GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
BstMWI GCNNNNNNNGC 8 cut(s) 14, 689, 778, 814, 835, 883, 1061, 1127
BstNI CCWGG 3 cut(s) 130, 223, 643
BstNSI RCATGY 2 cut(s) 113, 833
BstSCI CCNGG 3 cut(s) 128, 221, 641
BstSFI CTRYAG 1 cut(s) 839
BstV1I GCAGC 3 cut(s) 870, 925, 1105
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 2 cut(s) 496, 590
BtsCI GGATG 1 cut(s) 994
BtsIMutI CAGTG 2 cut(s) 44, 906
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 3 cut(s) 588, 705, 831
Cfr13I GGNCC 1 cut(s) 598
Csp6I GTAC 6 cut(s) 87, 390, 501, 696, 853, 1100
CviAII CATG 3 cut(s) 110, 830, 1061
CviQI GTAC 6 cut(s) 87, 390, 501, 696, 853, 1100
DdeI CTNAG 5 cut(s) 69, 564, 948, 1065, 1114
DpnI GATC 6 cut(s) 219, 297, 309, 408, 933, 1010
DpnII GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
Eam1104I CTCTTC 2 cut(s) 794, 1077
EarI CTCTTC 2 cut(s) 794, 1077
Eco147I AGGCCT 2 cut(s) 496, 590
Eco47I GGWCC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 1058
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 3 cut(s) 128, 221, 641
EcoT22I ATGCAT 1 cut(s) 573
FaeI CATG 3 cut(s) 113, 833, 1064
FalI AAGNNNNNCTT 4 cut(s) 367, 399, 575, 607
FaqI GGGAC 1 cut(s) 202
FatI CATG 3 cut(s) 109, 829, 1060
Fnu4HI GCNGC 4 cut(s) 255, 884, 914, 1119
FokI GGATG 1 cut(s) 981
Fsp4HI GCNGC 4 cut(s) 255, 884, 914, 1119
FspBI CTAG 6 cut(s) 230, 393, 489, 498, 704, 756
GluI GCNGC 4 cut(s) 255, 884, 914, 1119
GsuI CTGGAG 2 cut(s) 151, 794
HaeIII GGCC 2 cut(s) 496, 590
Hin1II CATG 3 cut(s) 113, 833, 1064
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 5 cut(s) 24, 378, 550, 896, 1133
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 478, 721
Hpy188I TCNGA 2 cut(s) 750, 949
Hpy188III TCNNGA 5 cut(s) 305, 353, 489, 625, 929
Hpy8I GTNNAC 2 cut(s) 478, 721
HpyAV CCTTC 6 cut(s) 48, 321, 486, 495, 526, 535
HpyCH4III ACNGT 2 cut(s) 100, 843
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 5 cut(s) 199, 571, 801, 913, 1055
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 689, 778, 814, 835, 883, 1061, 1127
HpyF3I CTNAG 5 cut(s) 69, 564, 948, 1065, 1114
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 3 cut(s) 113, 833, 1064
Kzo9I GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 2 cut(s) 135, 438
Lsp1109I GCAGC 3 cut(s) 870, 925, 1105
LweI GCATC 3 cut(s) 355, 558, 864
MaeI CTAG 6 cut(s) 230, 393, 489, 498, 704, 756
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 3 cut(s) 518, 663, 990
MalI GATC 6 cut(s) 219, 297, 309, 408, 933, 1010
MboI GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
MboII GAAGA 8 cut(s) 33, 290, 293, 368, 416, 541, 781, 1064
MflI RGATCY 1 cut(s) 217
MluCI AATT 5 cut(s) 155, 341, 637, 710, 795
MlyI GAGTC 1 cut(s) 1127
MmeI TCCRAC 1 cut(s) 195
Mph1103I ATGCAT 1 cut(s) 573
MseI TTAA 4 cut(s) 285, 330, 374, 507
MslI CAYNNNNRTG 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 808
MspR9I CCNGG 3 cut(s) 130, 223, 643
MvaI CCWGG 3 cut(s) 130, 223, 643
MwoI GCNNNNNNNGC 8 cut(s) 14, 689, 778, 814, 835, 883, 1061, 1127
NdeII GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
NheI GCTAGC 1 cut(s) 703
NlaIII CATG 3 cut(s) 113, 833, 1064
NlaIV GGNNCC 2 cut(s) 219, 1030
NmuCI GTSAC 2 cut(s) 518, 663
NsiI ATGCAT 1 cut(s) 573
NspI RCATGY 2 cut(s) 113, 833
NspV TTCGAA 2 cut(s) 792, 938
PaeI GCATGC 1 cut(s) 833
PasI CCCWGGG 1 cut(s) 222
PceI AGGCCT 2 cut(s) 496, 590
PciSI GCTCTTC 1 cut(s) 1077
PfeI GAWTC 4 cut(s) 24, 378, 550, 896
PflMI CCANNNNNTGG 1 cut(s) 648
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 4 cut(s) 256, 885, 915, 1120
PleI GAGTC 1 cut(s) 1127
PpsI GAGTC 1 cut(s) 1127
PpuMI RGGWCCY 1 cut(s) 598
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 3 cut(s) 128, 221, 641
PspGI CCWGG 3 cut(s) 128, 221, 641
PspN4I GGNNCC 2 cut(s) 219, 1030
PspPI GGNCC 1 cut(s) 598
PspPPI RGGWCCY 1 cut(s) 598
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 808
RsaI GTAC 6 cut(s) 88, 391, 502, 697, 854, 1101
RsaNI GTAC 6 cut(s) 87, 390, 501, 696, 853, 1100
RseI CAYNNNNRTG 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 4 cut(s) 285, 330, 374, 507
SatI GCNGC 4 cut(s) 255, 884, 914, 1119
Sau3AI GATC 6 cut(s) 217, 295, 307, 406, 931, 1008
Sau96I GGNCC 1 cut(s) 598
ScaI AGTACT 1 cut(s) 502
SchI GAGTC 1 cut(s) 1127
ScrFI CCNGG 3 cut(s) 130, 223, 643
SfaNI GCATC 3 cut(s) 355, 558, 864
SfcI CTRYAG 1 cut(s) 839
SfuI TTCGAA 2 cut(s) 792, 938
SinI GGWCC 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 2 cut(s) 165, 927
SmoI CTYRAG 2 cut(s) 165, 927
SphI GCATGC 1 cut(s) 833
Sse9I AATT 5 cut(s) 155, 341, 637, 710, 795
SseBI AGGCCT 2 cut(s) 496, 590
SsiI CCGC 1 cut(s) 254
SspMI CTAG 6 cut(s) 230, 393, 489, 498, 704, 756
StuI AGGCCT 2 cut(s) 496, 590
StyD4I CCNGG 3 cut(s) 128, 221, 641
TaaI ACNGT 2 cut(s) 100, 843
TaiI ACGT 1 cut(s) 92
TaqI TCGA 4 cut(s) 515, 715, 792, 938
TasI AATT 5 cut(s) 155, 341, 637, 710, 795
TatI WGTACW 3 cut(s) 500, 695, 852
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 4 cut(s) 24, 378, 550, 896
Tru1I TTAA 4 cut(s) 285, 330, 374, 507
Tru9I TTAA 4 cut(s) 285, 330, 374, 507
TscAI CASTG 2 cut(s) 51, 906
TseFI GTSAC 2 cut(s) 518, 663
TseI GCWGC 3 cut(s) 883, 913, 1118
Tsp45I GTSAC 2 cut(s) 518, 663
TspDTI ATGAA 1 cut(s) 339
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 2 cut(s) 51, 906
Van91I CCANNNNNTGG 1 cut(s) 648
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 3 cut(s) 637, 710, 795
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 2 cut(s) 113, 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XspI CTAG 6 cut(s) 230, 393, 489, 498, 704, 756
ZrmI AGTACT 1 cut(s) 502
Zsp2I ATGCAT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.