Rh4DG003500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000849
Physical Location & Seq
Reverse (-)
588454 .. 590704
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG003500.1

Sequence Viewer

Length: 639 bp
ATGAAGAATCTCCGGTTGAACCCTCTAATCCCAATCCTGGTGTTAAATTCCAACAACAACAACAACTTCAGATTCTCGCCGCGAAACCCGAAGATTTTGTCGCTCAGCGTTCTCGATTTTCCGGCGCTGGTGCTCAGCCCCGTCACGCCGCTCATACCCGACCCGTTTGACCGAAGAGACAGCGATCAAAGAAAAAGGGTTTGTCGCCAAGTACGACGCCGAGGGAATCGGAGCCCCGACTGTTGCCCTTATTCCCAACGAGTGGCTAAGCTCAAACTTGTATGGTGGCTTGACATCAACGCGTGCATATCTGCTAAGAACTTATCCCCGGGAACAACCTATGCAGCTTACCTTGTGTATCAGCTCTCAGAAGTAAGATCGGGGTTGGCAAGAACACCTATTGTGTTGCGCGTCAATTATCGACAAAGTGCAGTTGTGAGTGTGCATAGTGTGATCCTAGACCCTATGCCTCAGGAAGCTCGACATAGAGGAGATGGGTGGATGGAGATTGAGATGGGTCAATTCTTCATTGAACAAGGAAATGATGATGCTGCTGTAGAGTGCAGTGTAACGGAAGTTAGTAACCTAAAGAGCGGCCTCATTGTGGAAGGTATTGAGCTTAGGCCTATACATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

212

Amino Acids

24.02

Weight (kDa)

9.4

Isoelectric Point (pI)

61.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 86 - 209 1.9e-30 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 2 cut(s) 151, 594
AccII CGCG 3 cut(s) 82, 302, 411
AciI CCGC 3 cut(s) 80, 149, 594
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 1 cut(s) 213
AfiI CCNNNNNNNGG 3 cut(s) 37, 262, 604
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 2 cut(s) 19, 533
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 5 cut(s) 271, 347, 364, 479, 619
AluI AGCT 5 cut(s) 271, 347, 364, 479, 619
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 448
Ama87I CYCGRG 1 cut(s) 328
AoxI GGCC 2 cut(s) 595, 623
ApeKI GCWGC 2 cut(s) 344, 551
ApoI RAATTY 1 cut(s) 46
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
AspLEI GCGC 2 cut(s) 127, 411
AsuC2I CCSGG 2 cut(s) 329, 330
AvaI CYCGRG 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 471
BanII GRGCYC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 356, 538
BccI CCATC 3 cut(s) 488, 496, 508
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 2 cut(s) 329, 330
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 5 cut(s) 80, 149, 345, 552, 595
BlpI GCTNAGC 3 cut(s) 104, 134, 267
BlsI GCNGC 5 cut(s) 81, 150, 346, 553, 596
Bme1390I CCNGG 3 cut(s) 38, 329, 330
BmeT110I CYCGRG 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 3 cut(s) 38, 329, 330
BmsI GCATC 1 cut(s) 538
Bpu10I CCTNAGC 1 cut(s) 620
Bpu1102I GCTNAGC 3 cut(s) 104, 134, 267
BpuMI CCSGG 2 cut(s) 329, 330
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 220, 327, 328
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 262, 604
Bse21I CCTNAGG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 220, 327, 328
BseGI GGATG 1 cut(s) 507
BseLI CCNNNNNNNGG 3 cut(s) 37, 262, 604
BseMII CTCAG 4 cut(s) 118, 148, 381, 485
BseRI GAGGAG 1 cut(s) 504
BseXI GCAGC 2 cut(s) 356, 538
BsgI GTGCAG 2 cut(s) 450, 583
Bsh1236I CGCG 3 cut(s) 82, 302, 411
BshFI GGCC 2 cut(s) 597, 625
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 3 cut(s) 13, 122, 329
BslI CCNNNNNNNGG 3 cut(s) 37, 262, 604
BsmAI GTCTC 1 cut(s) 171
BsnI GGCC 2 cut(s) 597, 625
BsoBI CYCGRG 1 cut(s) 328
Bsp1286I GDGCHC 2 cut(s) 135, 236
Bsp143I GATC 3 cut(s) 184, 377, 453
Bsp1720I GCTNAGC 3 cut(s) 104, 134, 267
BspACI CCGC 3 cut(s) 80, 149, 594
BspANI GGCC 2 cut(s) 597, 625
BspCNI CTCAG 4 cut(s) 117, 147, 380, 484
BspFNI CGCG 3 cut(s) 82, 302, 411
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 1 cut(s) 448
BsrBI CCGCTC 2 cut(s) 151, 594
BssECI CCNNGG 3 cut(s) 220, 327, 328
BssMI GATC 3 cut(s) 184, 377, 453
BssNI GRCGYC 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 169
BstACI GRCGYC 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 7 cut(s) 104, 134, 267, 315, 367, 471, 620
BstF5I GGATG 1 cut(s) 507
BstFNI CGCG 3 cut(s) 82, 302, 411
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 127, 411
BstKTI GATC 3 cut(s) 187, 380, 456
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 184, 377, 453
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 36, 327, 328
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 3 cut(s) 82, 302, 411
BstV1I GCAGC 2 cut(s) 356, 538
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 2 cut(s) 597, 625
BtsCI GGATG 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 2 cut(s) 127, 411
Cfr9I CCCGGG 1 cut(s) 328
CseI GACGC 2 cut(s) 225, 400
Csp6I GTAC 1 cut(s) 212
CviQI GTAC 1 cut(s) 212
DdeI CTNAG 7 cut(s) 104, 134, 267, 315, 367, 471, 620
DpnI GATC 3 cut(s) 186, 379, 455
DpnII GATC 3 cut(s) 184, 377, 453
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 625
Eco24I GRGCYC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 52
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 236
FauNDI CATATG 1 cut(s) 633
Fnu4HI GCNGC 5 cut(s) 80, 149, 345, 552, 595
FokI GGATG 1 cut(s) 514
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 5 cut(s) 80, 149, 345, 552, 595
FspBI CTAG 1 cut(s) 458
GlaI GCGC 2 cut(s) 126, 410
GluI GCNGC 5 cut(s) 80, 149, 345, 552, 595
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 597, 625
HapII CCGG 3 cut(s) 13, 122, 329
HgaI GACGC 2 cut(s) 225, 400
HhaI GCGC 2 cut(s) 127, 411
Hin1I GRCGYC 1 cut(s) 217
Hin6I GCGC 2 cut(s) 125, 409
HinP1I GCGC 2 cut(s) 125, 409
HinfI GANTC 3 cut(s) 7, 72, 226
HpaII CCGG 3 cut(s) 13, 122, 329
Hpy188I TCNGA 3 cut(s) 71, 231, 370
Hpy188III TCNNGA 2 cut(s) 113, 473
Hpy99I CGWCG 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 602
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 5 cut(s) 306, 344, 431, 445, 564
HpyF3I CTNAG 7 cut(s) 104, 134, 267, 315, 367, 471, 620
Hsp92I GRCGYC 1 cut(s) 217
HspAI GCGC 2 cut(s) 125, 409
Kzo9I GATC 3 cut(s) 184, 377, 453
LmnI GCTCC 1 cut(s) 231
LpnPI CCDG 7 cut(s) 23, 26, 50, 113, 135, 342, 458
Lsp1109I GCAGC 2 cut(s) 356, 538
LweI GCATC 1 cut(s) 538
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 3 cut(s) 142, 568, 581
MalI GATC 3 cut(s) 186, 379, 455
MbiI CCGCTC 2 cut(s) 151, 594
MboI GATC 3 cut(s) 184, 377, 453
MboII GAAGA 4 cut(s) 16, 103, 186, 517
MhlI GDGCHC 2 cut(s) 135, 236
MluCI AATT 3 cut(s) 46, 415, 521
MluI ACGCGT 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 5 cut(s) 33, 215, 480, 482, 608
MseI TTAA 1 cut(s) 44
MspI CCGG 3 cut(s) 13, 122, 329
MspR9I CCNGG 3 cut(s) 38, 329, 330
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 3 cut(s) 82, 302, 411
NciI CCSGG 2 cut(s) 329, 330
NdeI CATATG 1 cut(s) 633
NdeII GATC 3 cut(s) 184, 377, 453
NlaIV GGNNCC 1 cut(s) 233
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 142
PceI AGGCCT 1 cut(s) 625
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PfeI GAWTC 3 cut(s) 7, 72, 226
PflMI CCANNNNNTGG 1 cut(s) 262
PkrI GCNGC 5 cut(s) 81, 150, 346, 553, 596
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 233
RsaI GTAC 1 cut(s) 213
RsaNI GTAC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 5 cut(s) 80, 149, 345, 552, 595
Sau3AI GATC 3 cut(s) 184, 377, 453
ScrFI CCNGG 3 cut(s) 38, 329, 330
SduI GDGCHC 2 cut(s) 135, 236
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 555
SmaI CCCGGG 1 cut(s) 330
Sse9I AATT 3 cut(s) 46, 415, 521
SseBI AGGCCT 1 cut(s) 625
SsiI CCGC 3 cut(s) 80, 149, 594
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 3 cut(s) 36, 327, 328
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 3 cut(s) 114, 421, 481
TaqII GACCGA 1 cut(s) 186
TasI AATT 3 cut(s) 46, 415, 521
TauI GCSGC 3 cut(s) 82, 151, 597
TfiI GAWTC 3 cut(s) 7, 72, 226
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 2 cut(s) 344, 551
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 2 cut(s) 17, 517
TspGWI ACGGA 1 cut(s) 587
TspMI CCCGGG 1 cut(s) 328
TspRI CASTG 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 262
XapI RAATTY 1 cut(s) 46
XmaI CCCGGG 1 cut(s) 328
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.