Rh4BG002900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000849
Physical Location & Seq
Reverse (-)
612833 .. 614115
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG002900.1

Sequence Viewer

Length: 963 bp
ATGCGAATATGTCATATATCTTTTGTTAGGGTTTATTTCAAAGTAGGTAGGTTGGTTGGTTTTGTTTCTGATAGACAGAAAATGGTGGGATTAGAGAGATTACCAGAAGAGTCATGCATAGCCCATGTTCTTTCCCTAACCTCCCCTAAAGATGCTTGCCGATCAGCCTTGGTCTCCTCTGCGTTTGGATCCGCCGCTCAATCCCAAGTTGTTTGGGAGCGTTTTTTACCCTCAGACCACCGTGACATCATCTCCCGATCCGTTTCTCCTGTCACCTTTTCCTCTAACAAGGAACTCTACTTCCGTCTCTGCGATTTTCCCATCTTCCTCGACGATGGTAATCTGAGTTTTGCAATAGATAAATTGAGTGGCAAAAAATGTTTGATGCTGGGAGCCAGACGTTTACTCATAGCCTGGGGAGACACTCCAATTTACTGGGAGTGGATTTCTGTGCCTCACTCCAGATTTGCCGAGGTGGCTTATCTGCACTCTGTTTGTTGGCTTGAGATTTATGGTAGATTGGAGATGCGATCCCTGTCTCCAAACACAGCTTATGCTGGATACTTGGTTTACATGTTCTCTGAAGAAGCCACTAATTACGCAGGAACTGGACAGAATTATTATGAAACTTTTCCGGTCAAGCTTTCAGTTAAGTTCTTTGAGAATGATGAAAATTTGATTGAAGCTCGTGACGAAGATGATGCAGAGTTGTTGATATCAACAAGAATGCCTGCTGCTGCTGCATCATCTATGAATAGTAGTGTTGAAAAGCGACAACGCTTTCCGATGGAGAGAGACGATGGCTGGATGGAGATTGAGATAGGAGAGTTTTATTCTGGCCAAAGTGATGATGACTATTGCTTGGTGGAGATGGGTGTCAAGGAGATCGAATCAGGGAGTTGGAAGTTTGGGCTTGTTATCCATGGCATTGAGATTCGGCCTATCCAACCGAAACCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

320

Amino Acids

36.45

Weight (kDa)

5.22

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 125 - 314 3.5e-47 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 197
AciI CCGC 2 cut(s) 192, 195
AclWI GGATC 4 cut(s) 183, 196, 252, 525
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 1 cut(s) 673
AcuI CTGAAG 1 cut(s) 603
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 3 cut(s) 40, 683, 767
AjnI CCWGG 1 cut(s) 413
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 3 cut(s) 551, 643, 686
AluI AGCT 3 cut(s) 551, 643, 686
Alw26I GTCTC 5 cut(s) 178, 311, 414, 543, 789
AlwI GGATC 4 cut(s) 183, 196, 252, 525
AlwNI CAGNNNCTG 1 cut(s) 608
AoxI GGCC 2 cut(s) 838, 938
ApeKI GCWGC 3 cut(s) 734, 737, 740
ApoI RAATTY 1 cut(s) 673
Asp700I GAANNNNTTC 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 265
BalI TGGCCA 1 cut(s) 840
BamHI GGATCC 1 cut(s) 188
BauI CACGAG 1 cut(s) 687
BbvI GCAGC 3 cut(s) 721, 724, 727
BccI CCATC 6 cut(s) 329, 329, 781, 794, 802, 865
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 1 cut(s) 415
BciVI GTATCC 1 cut(s) 554
BcoDI GTCTC 5 cut(s) 178, 311, 414, 543, 789
BfuI GTATCC 1 cut(s) 554
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 4 cut(s) 195, 735, 738, 741
BlsI GCNGC 4 cut(s) 196, 736, 739, 742
Bme1390I CCNGG 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 190, 394
BmrFI CCNGG 1 cut(s) 415
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 5 cut(s) 142, 375, 516, 691, 752
BmuI ACTGGG 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 445
BpuEI CTTGAG 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 4 cut(s) 168, 414, 471, 922
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bse1I ACTGG 2 cut(s) 440, 613
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 1 cut(s) 415
BseDI CCNNGG 4 cut(s) 168, 414, 471, 922
BseGI GGATG 1 cut(s) 813
BseJI GATNNNNATC 1 cut(s) 339
BseMII CTCAG 2 cut(s) 246, 335
BseNI ACTGG 2 cut(s) 440, 613
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 721, 724, 727
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 2 cut(s) 840, 940
BsiSI CCGG 1 cut(s) 635
BsmAI GTCTC 5 cut(s) 178, 311, 414, 543, 789
BsmBI CGTCTC 2 cut(s) 311, 789
BsmI GAATGC 1 cut(s) 732
BsnI GGCC 2 cut(s) 840, 940
Bso31I GGTCTC 1 cut(s) 178
Bsp143I GATC 5 cut(s) 161, 188, 257, 530, 885
Bsp19I CCATGG 1 cut(s) 922
BspACI CCGC 2 cut(s) 192, 195
BspANI GGCC 2 cut(s) 840, 940
BspCNI CTCAG 2 cut(s) 245, 336
BspLI GGNNCC 2 cut(s) 190, 394
BspPI GGATC 4 cut(s) 183, 196, 252, 525
BspTNI GGTCTC 1 cut(s) 178
BsrBI CCGCTC 1 cut(s) 197
BsrI ACTGG 2 cut(s) 440, 613
BssECI CCNNGG 4 cut(s) 168, 414, 471, 922
BssMI GATC 5 cut(s) 161, 188, 257, 530, 885
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 168, 922
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 157, 732
BstDEI CTNAG 2 cut(s) 232, 344
BstDSI CCRYGG 1 cut(s) 922
BstF5I GGATG 1 cut(s) 813
BstKTI GATC 5 cut(s) 164, 191, 260, 533, 888
BstMAI GTCTC 5 cut(s) 178, 311, 414, 543, 789
BstMBI GATC 5 cut(s) 161, 188, 257, 530, 885
BstMWI GCNNNNNNNGC 2 cut(s) 476, 740
BstNI CCWGG 1 cut(s) 415
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 413
BstV1I GCAGC 3 cut(s) 721, 724, 727
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 1 cut(s) 435
BstYI RGATCY 1 cut(s) 188
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 840, 940
BtgI CCRYGG 1 cut(s) 922
BtsCI GGATG 1 cut(s) 813
Cac8I GCNNGC 2 cut(s) 157, 732
CaiI CAGNNNCTG 1 cut(s) 608
CviAII CATG 4 cut(s) 114, 125, 574, 923
DdeI CTNAG 2 cut(s) 232, 344
DpnI GATC 5 cut(s) 163, 190, 259, 532, 887
DpnII GATC 5 cut(s) 161, 188, 257, 530, 885
EaeI YGGCCR 1 cut(s) 838
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 2 cut(s) 168, 922
Eco31I GGTCTC 1 cut(s) 178
Eco32I GATATC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 413
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 168, 922
EcoT22I ATGCAT 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 168, 922
Esp3I CGTCTC 2 cut(s) 311, 789
FaeI CATG 4 cut(s) 117, 128, 577, 926
FatI CATG 4 cut(s) 113, 124, 573, 922
Fnu4HI GCNGC 4 cut(s) 195, 735, 738, 741
FokI GGATG 1 cut(s) 820
Fsp4HI GCNGC 4 cut(s) 195, 735, 738, 741
GluI GCNGC 4 cut(s) 195, 735, 738, 741
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 2 cut(s) 840, 940
HapII CCGG 1 cut(s) 635
Hin1II CATG 4 cut(s) 117, 128, 577, 926
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 3 cut(s) 110, 890, 934
HpaII CCGG 1 cut(s) 635
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 2 cut(s) 404, 571
Hpy188I TCNGA 5 cut(s) 70, 235, 345, 583, 786
Hpy188III TCNNGA 3 cut(s) 255, 462, 689
Hpy8I GTNNAC 2 cut(s) 404, 571
Hpy99I CGWCG 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 5 cut(s) 117, 353, 487, 704, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 740
HpyF3I CTNAG 2 cut(s) 232, 344
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 4 cut(s) 117, 128, 577, 926
Kzo9I GATC 5 cut(s) 161, 188, 257, 530, 885
LmnI GCTCC 2 cut(s) 217, 392
Lsp1109I GCAGC 3 cut(s) 721, 724, 727
LweI GCATC 5 cut(s) 142, 375, 516, 691, 752
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 3 cut(s) 242, 271, 689
MalI GATC 5 cut(s) 163, 190, 259, 532, 887
MbiI CCGCTC 1 cut(s) 197
MboI GATC 5 cut(s) 161, 188, 257, 530, 885
MboII GAAGA 4 cut(s) 119, 316, 596, 707
MflI RGATCY 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 840
MluCI AATT 6 cut(s) 362, 429, 595, 616, 673, 958
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 881
MnlI CCTC 7 cut(s) 151, 187, 241, 292, 338, 465, 466
Mox20I TGGCCA 1 cut(s) 840
Mph1103I ATGCAT 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 840
MseI TTAA 2 cut(s) 651, 961
Msp20I TGGCCA 1 cut(s) 840
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 415
Mva1269I GAATGC 1 cut(s) 732
MvaI CCWGG 1 cut(s) 415
MwoI GCNNNNNNNGC 2 cut(s) 476, 740
NcoI CCATGG 1 cut(s) 922
NdeII GATC 5 cut(s) 161, 188, 257, 530, 885
NlaIII CATG 4 cut(s) 117, 128, 577, 926
NlaIV GGNNCC 2 cut(s) 190, 394
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 3 cut(s) 242, 271, 689
NsiI ATGCAT 1 cut(s) 119
NspI RCATGY 1 cut(s) 577
PciI ACATGT 1 cut(s) 573
PctI GAATGC 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 890, 934
PkrI GCNGC 4 cut(s) 196, 736, 739, 742
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PscI ACATGT 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 413
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 190, 394
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 188
SaqAI TTAA 2 cut(s) 651, 961
SatI GCNGC 4 cut(s) 195, 735, 738, 741
Sau3AI GATC 5 cut(s) 161, 188, 257, 530, 885
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 415
SetI ASST 9 cut(s) 49, 53, 143, 278, 403, 477, 553, 645, 688
SfaNI GCATC 5 cut(s) 142, 375, 516, 691, 752
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 6 cut(s) 362, 429, 595, 616, 673, 958
SsiI CCGC 2 cut(s) 192, 195
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 2 cut(s) 168, 922
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 330, 888
TasI AATT 6 cut(s) 362, 429, 595, 616, 673, 958
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 2 cut(s) 890, 934
Tru1I TTAA 2 cut(s) 651, 961
Tru9I TTAA 2 cut(s) 651, 961
TseFI GTSAC 3 cut(s) 242, 271, 689
TseI GCWGC 3 cut(s) 734, 737, 740
Tsp45I GTSAC 3 cut(s) 242, 271, 689
TspDTI ATGAA 3 cut(s) 639, 684, 767
TspGWI ACGGA 2 cut(s) 250, 293
XapI RAATTY 1 cut(s) 673
XceI RCATGY 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 630
Zsp2I ATGCAT 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.