Rorug03G0300400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000849
Physical Location & Seq
Forward (+)
31466498 .. 31467205
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0300400.1

Sequence Viewer

Length: 567 bp
ATGCCCATGACGTCGGCCCCCTCCCTCCCCGTCGCTACTGGCTATGCCGCCATCTCCGTGGAGCAAGAAGGAGTGGGTCCTCCCGGAAATCGACGAAAACGACATCGTCAGCGCCTCCCAGAGATCGAACTCGCTCCTCCGCCGCCTTCACCACACCTCGGAAAACGCTGTCTTCGCCGGAGATCATGGCGCCGGGGACGAGTTTATTCTGATCAACGATGGATTCGGCGGCCTTGTTCTTCCTTTGGCTCTGAGGCTCGAGAAGGAGAAGACGAAGGAGATGTTGAGGACGACCAGGATCTCAGCTTCCTCGAAGAGCCGACCATGATCTTGATCCGGATCAACCGGTCAGAGAAGTTATTGTCGTTGAAGGCGAAGGCGAAATCGGTGTCCGTCGACGTCGTCACCGTACCGCGAACTCTGACCCAATCATTCTACTATCTGCTATTTCCGATGCTCACAGTGGAGGAGATGCTCATGTTTTCCGACGCACTCTTCACATCCGATCCAGATTCAATTGAGCCACTCTTCACATCCGATTCAGATTCAATTGCATCTCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

188

Amino Acids

21.21

Weight (kDa)

5.8

Isoelectric Point (pI)

81.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 14, 402
AccB1I GGYRCC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 396
AccII CGCG 1 cut(s) 415
AccIII TCCGGA 1 cut(s) 336
AciI CCGC 5 cut(s) 48, 140, 143, 229, 413
AclWI GGATC 4 cut(s) 306, 328, 347, 500
AcyI GRCGYC 3 cut(s) 11, 190, 399
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 158
AgeI ACCGGT 1 cut(s) 345
AgsI TTSAA 3 cut(s) 370, 516, 549
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 1 cut(s) 306
AluI AGCT 1 cut(s) 306
AlwI GGATC 4 cut(s) 306, 328, 347, 500
Ama87I CYCGRG 1 cut(s) 258
Aor13HI TCCGGA 1 cut(s) 336
AoxI GGCC 2 cut(s) 15, 230
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AsiGI ACCGGT 1 cut(s) 345
AspLEI GCGC 2 cut(s) 114, 192
AspS9I GGNCC 2 cut(s) 16, 77
AsuC2I CCSGG 2 cut(s) 84, 194
AsuHPI GGTGA 2 cut(s) 141, 397
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 164, 276
BccI CCATC 2 cut(s) 59, 213
BciT130I CCWGG 1 cut(s) 296
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 84, 194
BfoI RGCGCY 2 cut(s) 115, 193
BisI GCNGC 3 cut(s) 48, 143, 230
BlsI GCNGC 3 cut(s) 49, 144, 231
Bme1390I CCNGG 3 cut(s) 84, 194, 296
Bme18I GGWCC 1 cut(s) 77
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 16, 77
BmiI GGNNCC 3 cut(s) 18, 78, 191
BmrFI CCNGG 3 cut(s) 84, 194, 296
BmsI GCATC 3 cut(s) 444, 462, 563
BpiI GAAGAC 2 cut(s) 164, 276
BpuMI CCSGG 2 cut(s) 84, 194
BsaBI GATNNNNATC 2 cut(s) 332, 338
BsaHI GRCGYC 3 cut(s) 11, 190, 399
BsaJI CCNNGG 3 cut(s) 57, 157, 193
BsaWI WCCGGW 2 cut(s) 336, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 43
Bse8I GATNNNNATC 2 cut(s) 332, 338
BseAI TCCGGA 1 cut(s) 336
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 57, 157, 193
BseGI GGATG 2 cut(s) 500, 533
BseJI GATNNNNATC 2 cut(s) 332, 338
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 2 cut(s) 243, 316
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 2 cut(s) 126, 482
Bsh1236I CGCG 1 cut(s) 415
BshFI GGCC 2 cut(s) 17, 232
BshNI GGYRCC 1 cut(s) 189
BshTI ACCGGT 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 5 cut(s) 84, 178, 193, 337, 346
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 2 cut(s) 17, 232
BsoBI CYCGRG 1 cut(s) 258
Bsp13I TCCGGA 1 cut(s) 336
Bsp143I GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
BspACI CCGC 5 cut(s) 48, 140, 143, 229, 413
BspANI GGCC 2 cut(s) 17, 232
BspCNI CTCAG 2 cut(s) 244, 315
BspEI TCCGGA 1 cut(s) 336
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 18, 78, 191
BspPI GGATC 4 cut(s) 306, 328, 347, 500
BspQI GCTCTTC 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 345
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 345
BssECI CCNNGG 3 cut(s) 57, 157, 193
BssMI GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
BssNI GRCGYC 3 cut(s) 11, 190, 399
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 409, 463
Bst6I CTCTTC 3 cut(s) 309, 500, 533
BstACI GRCGYC 3 cut(s) 11, 190, 399
BstDEI CTNAG 2 cut(s) 252, 302
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 500, 533
BstFNI CGCG 1 cut(s) 415
BstH2I RGCGCY 2 cut(s) 115, 193
BstHHI GCGC 2 cut(s) 114, 192
BstKTI GATC 8 cut(s) 126, 185, 214, 301, 330, 336, 342, 508
BstMBI GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 3 cut(s) 82, 192, 294
BstUI CGCG 1 cut(s) 415
BstV2I GAAGAC 2 cut(s) 164, 276
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 58
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 2 cut(s) 17, 232
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 500, 533
BtsIMutI CAGTG 1 cut(s) 468
CfoI GCGC 2 cut(s) 114, 192
Cfr10I RCCGGY 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 16, 77
CseI GACGC 1 cut(s) 497
Csp6I GTAC 1 cut(s) 410
CspAI ACCGGT 1 cut(s) 345
CviAII CATG 4 cut(s) 7, 186, 325, 478
CviJI RGCY 8 cut(s) 17, 42, 232, 249, 257, 306, 319, 523
CviKI_1 RGCY 8 cut(s) 17, 42, 232, 249, 257, 306, 319, 523
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 2 cut(s) 252, 302
DinI GGCGCC 1 cut(s) 191
DpnI GATC 8 cut(s) 125, 184, 213, 300, 329, 335, 341, 507
DpnII GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
Eam1104I CTCTTC 3 cut(s) 309, 500, 533
EarI CTCTTC 3 cut(s) 309, 500, 533
EciI GGCGGA 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 294
EgeI GGCGCC 1 cut(s) 191
EheI GGCGCC 1 cut(s) 191
FaeI CATG 4 cut(s) 10, 189, 328, 481
FaiI YATR 5 cut(s) 8, 45, 187, 326, 479
FaqI GGGAC 1 cut(s) 210
FatI CATG 4 cut(s) 6, 185, 324, 477
FbaI TGATCA 1 cut(s) 211
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 3 cut(s) 48, 143, 230
FokI GGATG 2 cut(s) 487, 520
Fsp4HI GCNGC 3 cut(s) 48, 143, 230
GlaI GCGC 2 cut(s) 113, 191
GluI GCNGC 3 cut(s) 48, 143, 230
HaeII RGCGCY 2 cut(s) 115, 193
HaeIII GGCC 2 cut(s) 17, 232
HapII CCGG 5 cut(s) 84, 178, 193, 337, 346
HgaI GACGC 1 cut(s) 497
HhaI GCGC 2 cut(s) 114, 192
Hin1I GRCGYC 3 cut(s) 11, 190, 399
Hin1II CATG 4 cut(s) 10, 189, 328, 481
Hin6I GCGC 2 cut(s) 112, 190
HinP1I GCGC 2 cut(s) 112, 190
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 4 cut(s) 223, 512, 539, 545
HpaII CCGG 5 cut(s) 84, 178, 193, 337, 346
HphI GGTGA 2 cut(s) 141, 397
Hpy166II GTNNAC 1 cut(s) 397
Hpy188III TCNNGA 4 cut(s) 260, 331, 337, 509
Hpy8I GTNNAC 1 cut(s) 397
Hpy99I CGWCG 7 cut(s) 16, 35, 96, 398, 401, 404, 491
HpyAV CCTTC 6 cut(s) 62, 156, 257, 269, 364, 370
HpyCH4III ACNGT 2 cut(s) 409, 463
HpyCH4IV ACGT 2 cut(s) 11, 399
HpyCH4V TGCA 1 cut(s) 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 2 cut(s) 252, 302
HpySE526I ACGT 2 cut(s) 11, 399
Hsp92I GRCGYC 3 cut(s) 11, 190, 399
Hsp92II CATG 4 cut(s) 10, 189, 328, 481
HspAI GCGC 2 cut(s) 112, 190
KasI GGCGCC 1 cut(s) 189
Kpn2I TCCGGA 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 2 cut(s) 61, 139
LweI GCATC 3 cut(s) 444, 462, 563
MaeII ACGT 2 cut(s) 11, 399
MaeIII GTNAC 1 cut(s) 403
MalI GATC 8 cut(s) 125, 184, 213, 300, 329, 335, 341, 507
MboI GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
MboII GAAGA 6 cut(s) 164, 231, 281, 326, 487, 520
MfeI CAATTG 2 cut(s) 516, 549
MflI RGATCY 1 cut(s) 298
MluCI AATT 2 cut(s) 516, 549
Mly113I GGCGCC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 510
MroI TCCGGA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 5 cut(s) 84, 178, 193, 337, 346
MspR9I CCNGG 3 cut(s) 84, 194, 296
MunI CAATTG 2 cut(s) 516, 549
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 1 cut(s) 174
NarI GGCGCC 1 cut(s) 190
NciI CCSGG 2 cut(s) 84, 194
NdeII GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
NlaIII CATG 4 cut(s) 10, 189, 328, 481
NlaIV GGNNCC 3 cut(s) 18, 78, 191
NmuCI GTSAC 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 309
PcsI WCGNNNNNNNCGW 3 cut(s) 97, 371, 405
PfeI GAWTC 4 cut(s) 223, 512, 539, 545
PflFI GACNNNGTC 2 cut(s) 105, 401
PfoI TCCNGGA 1 cut(s) 82
PinAI ACCGGT 1 cut(s) 345
PkrI GCNGC 3 cut(s) 49, 144, 231
PluTI GGCGCC 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 18, 78, 191
PspPI GGNCC 2 cut(s) 16, 77
PspPPI RGGWCCY 1 cut(s) 77
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 2 cut(s) 105, 401
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 56
SalI GTCGAC 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 309
SatI GCNGC 3 cut(s) 48, 143, 230
Sau3AI GATC 8 cut(s) 123, 182, 211, 298, 327, 333, 339, 505
Sau96I GGNCC 2 cut(s) 16, 77
ScrFI CCNGG 3 cut(s) 84, 194, 296
SetI ASST 4 cut(s) 14, 159, 308, 402
SfaNI GCATC 3 cut(s) 444, 462, 563
SfoI GGCGCC 1 cut(s) 191
Sfr274I CTCGAG 1 cut(s) 258
SgrDI CGTCGACG 1 cut(s) 395
SinI GGWCC 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
Sse9I AATT 2 cut(s) 516, 549
SsiI CCGC 5 cut(s) 48, 140, 143, 229, 413
SspDI GGCGCC 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 82, 192, 294
TaaI ACNGT 2 cut(s) 409, 463
TaiI ACGT 2 cut(s) 14, 402
TaqI TCGA 5 cut(s) 91, 126, 259, 312, 396
TasI AATT 2 cut(s) 516, 549
TauI GCSGC 3 cut(s) 50, 145, 232
TfiI GAWTC 4 cut(s) 223, 512, 539, 545
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 403
TspGWI ACGGA 2 cut(s) 46, 382
TspRI CASTG 1 cut(s) 468
Tth111I GACNNNGTC 2 cut(s) 105, 401
VpaK11BI GGWCC 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 396
ZraI GACGTC 2 cut(s) 12, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.