Rh4AG003600

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000849
Physical Location & Seq
Reverse (-)
584958 .. 598282
13325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG003600.1

Sequence Viewer

Length: 1236 bp
ATGGAGTTAACAGATTTGGAGAAGTTACCGGAGGAGTGCATGGCCCATATTCTTTCCCTCACGTCCCCCAAAGACGCGTGCAGATCAGCCGCCGTCTCCTCCGCCTTTCGCTCCGCCGCGCAATCCCAAACTCTTTGGGAACGTTTCTTACCCCATGACTATCGTCAAATCATCTCTCGATCCGTTTCTCCTCCTCTGCCATCAATCTCCTCTCTCTCTAACAAGGACCTCTACTTCCGTCTCTGCGACTCCCCCATCTTCCTCGACGATGGCAATTTGAGTTTCTCATTCGATAAATTGAGTGGTAAAAAATGTTGGATGCTGGGAGCCAGACTGTTGAGCATTACATGGGGAGACACTCCAGATTACTGGGAATGGAATTCTCTACCCGAATCCAGGTTCTCCGAAGTGGCTCAGCTCAGACTTGTATGGTGGCTTGAGATTAAGGGAAACATAGACACTAAAATTTTGTCCCCCCGAACAACCTACGCAGCTTACCTTGTATATCAGTGTCCAGAAGAATTACAATGGGGGTTTCAGGGAATGCCTATGACGGTGCGTGTCACCAATGAACAAAATGAAGTTGTAGCTGAGCATGATGTGTTCCTAGCAGACCCGCCAGAAGACATGCCTCCACAAGCTCGAAGCAGAGGAGATGGATGGATGGAGATTGAGTTGGGTGAAATTTTCAACCAAGAAGATGATGTTACTGTGGAGTTTCGTCTAAGGGAAACTGAGAGTGGCGAGCCTAAGGGCGGCCTCATTGTGGAAGTGAAGAGAATGCAGAGGTCATTAACCACACAACTAAGAAACTTCACCACCACACTGCCTTATCAGTCCATTAATCTTTTTTCTTTGCCGAGACATGGAAAGATTTATATTCCAAGTGGTTTTAGATTGACTGGATTAGGGTCACCACCTGTGAATCACTTCAAAAAACATCATCAGCCAAATCAGCTTATTGCTGTCAATGTTATTCCATTGGACAAAAATCCACGTTCAGTGTTCAATGCTGCCAATGCTTTTTTGTTGGTCAAATATCAAAGTATAGTAAGGCATCAGTGCAGTACAATGGCGGCTTCATCCAAACGGAAAGTAAAAAGATGTTCTATTCTCCATGATACAGTTGCCTATATGATAAAAAATTACTGTGTGGATATGAACTGGACAGTACATGGTGGACAAAGTGATGAACAACTGAAACAATCATTTAAAATACAACATAGAGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

411

Amino Acids

46.98

Weight (kDa)

6.84

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 47 3.9e-07 F-box domain
F-box-like PF12937 7 - 49 3.5e-09 F-box-like
PP2 PF14299 103 - 257 4.8e-47 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 77, 119
AciI CCGC 7 cut(s) 90, 102, 114, 117, 617, 756, 1076
AclI AACGTT 1 cut(s) 142
AclWI GGATC 1 cut(s) 174
AcsI RAATTY 3 cut(s) 379, 465, 684
AfaI GTAC 2 cut(s) 1069, 1173
AfiI CCNNNNNNNGG 4 cut(s) 396, 755, 766, 866
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 691, 934, 1009
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 1 cut(s) 395
AluBI AGCT 5 cut(s) 418, 494, 590, 641, 958
AluI AGCT 5 cut(s) 418, 494, 590, 641, 958
Alw26I GTCTC 4 cut(s) 100, 245, 348, 856
AlwI GGATC 1 cut(s) 174
AoxI GGCC 2 cut(s) 42, 757
ApeKI GCWGC 2 cut(s) 491, 1013
ApoI RAATTY 3 cut(s) 379, 465, 684
AseI ATTAAT 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 929
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 2 cut(s) 43, 226
AsuHPI GGTGA 4 cut(s) 556, 692, 808, 906
AvaII GGWCC 1 cut(s) 226
AxyI CCTNAGG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 2 cut(s) 503, 1000
BccI CCATC 6 cut(s) 208, 263, 263, 650, 654, 658
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 397
BcoDI GTCTC 4 cut(s) 100, 245, 348, 856
BfaI CTAG 1 cut(s) 608
BisI GCNGC 6 cut(s) 90, 117, 492, 757, 1014, 1077
BlpI GCTNAGC 2 cut(s) 414, 591
BlsI GCNGC 6 cut(s) 91, 118, 493, 758, 1015, 1078
Bme1390I CCNGG 1 cut(s) 397
Bme18I GGWCC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 43, 226
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 397
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 309, 1066
BmuI ACTGGG 1 cut(s) 379
BoxI GACNNNNGTC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 345
Bpu1102I GCTNAGC 2 cut(s) 414, 591
BpuEI CTTGAG 1 cut(s) 458
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 4 cut(s) 396, 755, 766, 866
Bse1I ACTGG 3 cut(s) 374, 907, 1169
Bse21I CCTNAGG 1 cut(s) 750
BseBI CCWGG 1 cut(s) 397
BseGI GGATG 4 cut(s) 324, 665, 669, 1082
BseLI CCNNNNNNNGG 4 cut(s) 396, 755, 766, 866
BseMII CTCAG 4 cut(s) 428, 433, 582, 726
BseNI ACTGG 3 cut(s) 374, 907, 1169
BseRI GAGGAG 6 cut(s) 47, 88, 180, 183, 199, 666
BseXI GCAGC 2 cut(s) 503, 1000
BseYI CCCAGC 1 cut(s) 322
BsgI GTGCAG 2 cut(s) 100, 1084
Bsh1236I CGCG 2 cut(s) 77, 119
BshFI GGCC 2 cut(s) 44, 759
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 49, 457
BslI CCNNNNNNNGG 4 cut(s) 396, 755, 766, 866
BsmAI GTCTC 4 cut(s) 100, 245, 348, 856
BsmBI CGTCTC 2 cut(s) 100, 245
BsmFI GGGAC 2 cut(s) 49, 457
BsmI GAATGC 2 cut(s) 549, 786
BsnI GGCC 2 cut(s) 44, 759
Bsp143I GATC 2 cut(s) 83, 179
Bsp1720I GCTNAGC 2 cut(s) 414, 591
BspACI CCGC 7 cut(s) 90, 102, 114, 117, 617, 756, 1076
BspANI GGCC 2 cut(s) 44, 759
BspCNI CTCAG 4 cut(s) 427, 432, 583, 727
BspFNI CGCG 2 cut(s) 77, 119
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 1 cut(s) 174
BsrI ACTGG 3 cut(s) 374, 907, 1169
BssMI GATC 2 cut(s) 83, 179
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 6 cut(s) 336, 556, 712, 1126, 1151, 1171
Bst6I CTCTTC 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 79, 746
BstDEI CTNAG 7 cut(s) 414, 419, 591, 725, 735, 750, 806
BstEII GGTNACC 1 cut(s) 912
BstF5I GGATG 4 cut(s) 324, 665, 669, 1082
BstFNI CGCG 2 cut(s) 77, 119
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 2 cut(s) 86, 182
BstMAI GTCTC 4 cut(s) 100, 245, 348, 856
BstMBI GATC 2 cut(s) 83, 179
BstMWI GCNNNNNNNGC 2 cut(s) 955, 1019
BstNI CCWGG 1 cut(s) 397
BstNSI RCATGY 1 cut(s) 631
BstPAI GACNNNNGTC 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 912
BstSCI CCNGG 1 cut(s) 395
BstUI CGCG 2 cut(s) 77, 119
BstV1I GCAGC 2 cut(s) 503, 1000
BstV2I GAAGAC 1 cut(s) 630
BstXI CCANNNNNNTGG 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 750
BsuRI GGCC 2 cut(s) 44, 759
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 4 cut(s) 324, 665, 669, 1082
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 4 cut(s) 515, 824, 1008, 1067
Cac8I GCNNGC 2 cut(s) 79, 746
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 43, 226
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 1068, 1172
CviAII CATG 8 cut(s) 40, 155, 348, 596, 628, 866, 1118, 1175
CviQI GTAC 2 cut(s) 1068, 1172
DdeI CTNAG 7 cut(s) 414, 419, 591, 725, 735, 750, 806
DpnI GATC 2 cut(s) 85, 181
DpnII GATC 2 cut(s) 83, 179
DraI TTTAAA 1 cut(s) 1213
Eam1104I CTCTTC 1 cut(s) 770
EarI CTCTTC 1 cut(s) 770
EciI GGCGGA 2 cut(s) 91, 103
Eco47I GGWCC 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 750
Eco91I GGTNACC 1 cut(s) 912
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 912
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 395
Esp3I CGTCTC 2 cut(s) 100, 245
FaeI CATG 8 cut(s) 43, 158, 351, 599, 631, 869, 1121, 1178
FaqI GGGAC 2 cut(s) 49, 457
FatI CATG 8 cut(s) 39, 154, 347, 595, 627, 865, 1117, 1174
FauI CCCGC 1 cut(s) 624
Fnu4HI GCNGC 6 cut(s) 90, 117, 492, 757, 1014, 1077
FokI GGATG 4 cut(s) 331, 672, 676, 1069
Fsp4HI GCNGC 6 cut(s) 90, 117, 492, 757, 1014, 1077
FspBI CTAG 1 cut(s) 608
GlaI GCGC 1 cut(s) 120
GluI GCNGC 6 cut(s) 90, 117, 492, 757, 1014, 1077
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 2 cut(s) 44, 759
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 121
Hin1II CATG 8 cut(s) 43, 158, 351, 599, 631, 869, 1121, 1178
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 3 cut(s) 248, 392, 925
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 29
HphI GGTGA 4 cut(s) 556, 692, 808, 906
Hpy166II GTNNAC 2 cut(s) 9, 1181
Hpy188I TCNGA 2 cut(s) 406, 422
Hpy188III TCNNGA 3 cut(s) 177, 362, 515
Hpy8I GTNNAC 2 cut(s) 9, 1181
Hpy99I CGWCG 1 cut(s) 269
HpyCH4III ACNGT 6 cut(s) 336, 556, 712, 1126, 1151, 1171
HpyCH4IV ACGT 3 cut(s) 62, 142, 997
HpyCH4V TGCA 4 cut(s) 39, 81, 784, 1065
HpyF10VI GCNNNNNNNGC 2 cut(s) 955, 1019
HpyF3I CTNAG 7 cut(s) 414, 419, 591, 725, 735, 750, 806
HpySE526I ACGT 3 cut(s) 62, 142, 997
Hsp92II CATG 8 cut(s) 43, 158, 351, 599, 631, 869, 1121, 1178
HspAI GCGC 1 cut(s) 119
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 2 cut(s) 83, 179
LmnI GCTCC 2 cut(s) 116, 326
Lsp1109I GCAGC 2 cut(s) 503, 1000
LweI GCATC 2 cut(s) 309, 1066
MaeI CTAG 1 cut(s) 608
MaeII ACGT 3 cut(s) 62, 142, 997
MaeIII GTNAC 4 cut(s) 24, 562, 706, 912
MalI GATC 2 cut(s) 85, 181
MboI GATC 2 cut(s) 83, 179
MboII GAAGA 5 cut(s) 250, 530, 635, 710, 787
MluCI AATT 7 cut(s) 274, 296, 379, 465, 521, 684, 1144
MluI ACGCGT 1 cut(s) 75
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 929
MseI TTAA 5 cut(s) 8, 444, 794, 843, 1212
MslI CAYNNNNRTG 1 cut(s) 1227
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 397
Mva1269I GAATGC 2 cut(s) 549, 786
MvaI CCWGG 1 cut(s) 397
MvnI CGCG 2 cut(s) 77, 119
MwoI GCNNNNNNNGC 2 cut(s) 955, 1019
NdeII GATC 2 cut(s) 83, 179
NlaIII CATG 8 cut(s) 43, 158, 351, 599, 631, 869, 1121, 1178
NlaIV GGNNCC 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 885
NmuCI GTSAC 2 cut(s) 562, 912
NspI RCATGY 1 cut(s) 631
PctI GAATGC 2 cut(s) 549, 786
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 2 cut(s) 392, 925
PkrI GCNGC 6 cut(s) 91, 118, 493, 758, 1015, 1078
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PpuMI RGGWCCY 1 cut(s) 226
PshAI GACNNNNGTC 1 cut(s) 162
PshBI ATTAAT 1 cut(s) 843
Psp1406I AACGTT 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 395
PspEI GGTNACC 1 cut(s) 912
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 395
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 43, 226
PspPPI RGGWCCY 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 132, 164
RsaI GTAC 2 cut(s) 1069, 1173
RsaNI GTAC 2 cut(s) 1068, 1172
RseI CAYNNNNRTG 1 cut(s) 1227
SaqAI TTAA 5 cut(s) 8, 444, 794, 843, 1212
SatI GCNGC 6 cut(s) 90, 117, 492, 757, 1014, 1077
Sau3AI GATC 2 cut(s) 83, 179
Sau96I GGNCC 2 cut(s) 43, 226
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 397
SfaNI GCATC 2 cut(s) 309, 1066
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 1227
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 7 cut(s) 274, 296, 379, 465, 521, 684, 1144
SsiI CCGC 7 cut(s) 90, 102, 114, 117, 617, 756, 1076
SspMI CTAG 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 395
TaaI ACNGT 6 cut(s) 336, 556, 712, 1126, 1151, 1171
TaiI ACGT 3 cut(s) 65, 145, 1000
TaqI TCGA 4 cut(s) 178, 264, 291, 643
TasI AATT 7 cut(s) 274, 296, 379, 465, 521, 684, 1144
TatI WGTACW 2 cut(s) 1067, 1171
TauI GCSGC 4 cut(s) 92, 119, 759, 1079
TfiI GAWTC 2 cut(s) 392, 925
Tru1I TTAA 5 cut(s) 8, 444, 794, 843, 1212
Tru9I TTAA 5 cut(s) 8, 444, 794, 843, 1212
TscAI CASTG 4 cut(s) 515, 831, 1008, 1067
TseFI GTSAC 2 cut(s) 562, 912
TseI GCWGC 2 cut(s) 491, 1013
Tsp45I GTSAC 2 cut(s) 562, 912
TspDTI ATGAA 5 cut(s) 585, 594, 1071, 1175, 1206
TspGWI ACGGA 3 cut(s) 172, 227, 1105
TspRI CASTG 4 cut(s) 515, 831, 1008, 1067
VpaK11BI GGWCC 1 cut(s) 226
VspI ATTAAT 1 cut(s) 843
XapI RAATTY 3 cut(s) 379, 465, 684
XceI RCATGY 1 cut(s) 631
XmnI GAANNNNTTC 1 cut(s) 929
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.