FvH4_4g06723

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002041
Physical Location & Seq
Forward (+)
6048745 .. 6055874
7130 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06723.t1

Sequence Viewer

Length: 759 bp
ATGCGTCGGAGTCGGCCTCCAAATCGGTGGAGGTTGGGTTGCTTGGATGTGGATGTCAGCTTGTTTTACCCTGCCGAACGATGTGCTTCCAATAACGGTGGGCAAGATATGGTGGTGGGCTCTGGCTCTCGGGTCTCGGGCTCAAATGGGGCAATGATGGGCTTCATCGGAGGAGTGACTTCTGACCAGGCTGCAGGACGTACAAGTTCTAACTCGCAAGGGAAAAGACTTCATAATTTGGTTTTTAGTTTGGAGAATGGAGACAAGGAGGAGCAAGTTGCCAACTTGGCGTTGACCTTTCAAGGTCAAGACCAGCCAGTGCAGAGTGGGACACGAAGACCAGCCAGTGCAGAGTGGAACACGTGGGGTGCAGCAGTCCATGAGCAGAGTGACATTTTTCCGGAATATTCCAGAAATGTCGGTCATCCTAGAAGAGATATAATTGTTCAAATTTTGAAAAATTCTCATGGACATCTATTCTTCCGCTCAAAACGAAGACATTCGGGATCTAGCTTCTCCATGAAGCATGATGGTGACGCCATGGTTGTAAATGGTGGCACCCGGGGGACATTGACGTCACCCGAACACGGCTCCAACTTCCTGGAAGCCGTCCGGTCAATTCCTCAAGCAATTGAGGTGCACCGGCCTTCCCTCGATGTCTCATTGGCCCCCTGCCACTGGACTCCGGTCACCGATCTCCGGTTATTGACCCCTACTAACTCAAATTTTCTCCCGTCGCCGGATTTCGGTCACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

253

Amino Acids

27.6

Weight (kDa)

9.25

Isoelectric Point (pI)

54.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 574
AatII GACGTC 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 557
AccBSI CCGCTC 1 cut(s) 486
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 1 cut(s) 484
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 3 cut(s) 450, 460, 724
AcvI CACGTG 1 cut(s) 363
AcyI GRCGYC 2 cut(s) 537, 575
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 5 cut(s) 587, 678, 699, 739, 746
AflIII ACRYGT 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 753
AgsI TTSAA 3 cut(s) 302, 449, 457
AjnI CCWGG 2 cut(s) 186, 600
AluBI AGCT 2 cut(s) 60, 513
AluI AGCT 2 cut(s) 60, 513
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 3 cut(s) 139, 255, 664
Alw44I GTGCAC 1 cut(s) 638
AlwI GGATC 1 cut(s) 514
Ama87I CYCGRG 3 cut(s) 129, 136, 561
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 14, 644, 666
ApaLI GTGCAC 1 cut(s) 638
ApeKI GCWGC 2 cut(s) 191, 371
ApoI RAATTY 3 cut(s) 450, 460, 724
AsiGI ACCGGT 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 499
AspS9I GGNCC 1 cut(s) 667
AsuC2I CCSGG 2 cut(s) 562, 563
AsuHPI GGTGA 4 cut(s) 545, 570, 682, 743
AvaI CYCGRG 3 cut(s) 129, 136, 561
BaeGI GKGCMC 1 cut(s) 642
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 122, 143
BbrPI CACGTG 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 343, 502
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 178, 383
BccI CCATC 2 cut(s) 151, 524
BceAI ACGGC 2 cut(s) 593, 604
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 2 cut(s) 188, 602
BcnI CCSGG 2 cut(s) 562, 563
BcoDI GTCTC 3 cut(s) 139, 255, 664
BfaI CTAG 2 cut(s) 429, 510
BfmI CTRYAG 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 2 cut(s) 192, 372
BlsI GCNGC 2 cut(s) 193, 373
Bme1390I CCNGG 4 cut(s) 188, 562, 563, 602
BmeT110I CYCGRG 3 cut(s) 129, 136, 561
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 3 cut(s) 559, 592, 669
BmrFI CCNGG 4 cut(s) 188, 562, 563, 602
BoxI GACNNNNGTC 1 cut(s) 686
BpiI GAAGAC 2 cut(s) 343, 502
BplI GAGNNNNNCTC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 609
BpuMI CCSGG 2 cut(s) 562, 563
BsaAI YACGTR 1 cut(s) 363
BsaHI GRCGYC 2 cut(s) 537, 575
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 540, 561, 562
BsaWI WCCGGW 5 cut(s) 400, 612, 685, 699, 753
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 5 cut(s) 587, 678, 699, 739, 746
Bse118I RCCGGY 2 cut(s) 642, 753
Bse1I ACTGG 3 cut(s) 317, 345, 683
Bse3DI GCAATG 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 2 cut(s) 188, 602
BseDI CCNNGG 3 cut(s) 540, 561, 562
BseGI GGATG 3 cut(s) 52, 58, 424
BseLI CCNNNNNNNGG 5 cut(s) 587, 678, 699, 739, 746
BseMI GCAATG 1 cut(s) 159
BseNI ACTGG 3 cut(s) 317, 345, 683
BseRI GAGGAG 2 cut(s) 186, 284
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 2 cut(s) 178, 383
BsgI GTGCAG 3 cut(s) 341, 369, 390
BshFI GGCC 3 cut(s) 16, 646, 668
BshNI GGYRCC 1 cut(s) 557
BshTI ACCGGT 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 642
BsiHKCI CYCGRG 3 cut(s) 129, 136, 561
BsiSI CCGG 8 cut(s) 401, 562, 613, 643, 686, 700, 740, 754
BslFI GGGAC 2 cut(s) 343, 580
BslI CCNNNNNNNGG 5 cut(s) 587, 678, 699, 739, 746
BsmAI GTCTC 3 cut(s) 139, 255, 664
BsmFI GGGAC 2 cut(s) 343, 580
BsnI GGCC 3 cut(s) 16, 646, 668
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 3 cut(s) 129, 136, 561
Bsp1286I GDGCHC 3 cut(s) 122, 143, 642
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 506, 694
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 1 cut(s) 484
BspANI GGCC 3 cut(s) 16, 646, 668
BspEI TCCGGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 559, 592, 669
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 642, 753
BsrI ACTGG 3 cut(s) 317, 345, 683
BssAI RCCGGY 2 cut(s) 642, 753
BssECI CCNNGG 3 cut(s) 540, 561, 562
BssMI GATC 2 cut(s) 506, 694
BssNI GRCGYC 2 cut(s) 537, 575
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 188, 602
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 427
BstACI GRCGYC 2 cut(s) 537, 575
BstBAI YACGTR 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 540
BstEII GGTNACC 2 cut(s) 688, 749
BstF5I GGATG 3 cut(s) 52, 58, 424
BstKTI GATC 2 cut(s) 509, 697
BstMAI GTCTC 3 cut(s) 139, 255, 664
BstMBI GATC 2 cut(s) 506, 694
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 2 cut(s) 188, 602
BstPAI GACNNNNGTC 1 cut(s) 686
BstPI GGTNACC 2 cut(s) 688, 749
BstSCI CCNGG 4 cut(s) 186, 560, 561, 600
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 642
BstV1I GCAGC 2 cut(s) 178, 383
BstV2I GAAGAC 2 cut(s) 343, 502
BstX2I RGATCY 1 cut(s) 506
BstXI CCANNNNNNTGG 2 cut(s) 27, 601
BstYI RGATCY 1 cut(s) 506
BsuRI GGCC 3 cut(s) 16, 646, 668
BtgI CCRYGG 1 cut(s) 540
BtsCI GGATG 3 cut(s) 52, 58, 424
BtsIMutI CAGTG 3 cut(s) 324, 352, 676
Cfr10I RCCGGY 2 cut(s) 642, 753
Cfr13I GGNCC 1 cut(s) 667
Cfr9I CCCGGG 1 cut(s) 561
CseI GACGC 1 cut(s) 545
Csp6I GTAC 1 cut(s) 201
CspAI ACCGGT 1 cut(s) 753
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 5 cut(s) 380, 467, 520, 527, 541
CviQI GTAC 1 cut(s) 201
DpnI GATC 2 cut(s) 508, 696
DpnII GATC 2 cut(s) 506, 694
DrdI GACNNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 574
Eam1104I CTCTTC 1 cut(s) 427
EarI CTCTTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 2 cut(s) 122, 143
Eco31I GGTCTC 1 cut(s) 139
Eco72I CACGTG 1 cut(s) 363
Eco88I CYCGRG 3 cut(s) 129, 136, 561
Eco91I GGTNACC 2 cut(s) 688, 749
EcoO65I GGTNACC 2 cut(s) 688, 749
EcoRII CCWGG 2 cut(s) 186, 600
EcoT14I CCWWGG 1 cut(s) 540
EcoT38I GRGCYC 2 cut(s) 122, 143
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 5 cut(s) 383, 470, 523, 530, 544
FaiI YATR 8 cut(s) 110, 234, 381, 440, 468, 521, 528, 542
FaqI GGGAC 2 cut(s) 343, 580
FatI CATG 5 cut(s) 379, 466, 519, 526, 540
Fnu4HI GCNGC 2 cut(s) 192, 372
FokI GGATG 3 cut(s) 59, 65, 411
FriOI GRGCYC 2 cut(s) 122, 143
Fsp4HI GCNGC 2 cut(s) 192, 372
FspBI CTAG 2 cut(s) 429, 510
GluI GCNGC 2 cut(s) 192, 372
HaeIII GGCC 3 cut(s) 16, 646, 668
HapII CCGG 8 cut(s) 401, 562, 613, 643, 686, 700, 740, 754
HgaI GACGC 1 cut(s) 545
Hin1I GRCGYC 2 cut(s) 537, 575
Hin1II CATG 5 cut(s) 383, 470, 523, 530, 544
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 2 cut(s) 10, 682
HpaII CCGG 8 cut(s) 401, 562, 613, 643, 686, 700, 740, 754
HphI GGTGA 4 cut(s) 545, 570, 682, 743
Hpy166II GTNNAC 2 cut(s) 294, 640
Hpy188I TCNGA 3 cut(s) 9, 170, 184
Hpy188III TCNNGA 4 cut(s) 308, 401, 411, 504
Hpy8I GTNNAC 2 cut(s) 294, 640
Hpy99I CGWCG 2 cut(s) 9, 739
HpyAV CCTTC 1 cut(s) 657
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 3 cut(s) 199, 362, 575
HpyCH4V TGCA 5 cut(s) 194, 322, 350, 371, 640
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpySE526I ACGT 3 cut(s) 199, 362, 575
Hsp92I GRCGYC 2 cut(s) 537, 575
Hsp92II CATG 5 cut(s) 383, 470, 523, 530, 544
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 2 cut(s) 506, 694
LmnI GCTCC 2 cut(s) 271, 596
Lsp1109I GCAGC 2 cut(s) 178, 383
MaeI CTAG 2 cut(s) 429, 510
MaeII ACGT 3 cut(s) 199, 362, 575
MaeIII GTNAC 6 cut(s) 175, 389, 533, 576, 688, 749
MalI GATC 2 cut(s) 508, 696
MbiI CCGCTC 1 cut(s) 486
MboI GATC 2 cut(s) 506, 694
MboII GAAGA 4 cut(s) 348, 444, 472, 507
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 3 cut(s) 122, 143, 642
MluCI AATT 7 cut(s) 235, 441, 450, 460, 618, 630, 724
MlyI GAGTC 2 cut(s) 19, 676
MmeI TCCRAC 1 cut(s) 618
MnlI CCTC 7 cut(s) 24, 27, 164, 262, 628, 633, 662
MroI TCCGGA 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 499
MslI CAYNNNNRTG 1 cut(s) 531
MspI CCGG 8 cut(s) 401, 562, 613, 643, 686, 700, 740, 754
MspR9I CCNGG 4 cut(s) 188, 562, 563, 602
MunI CAATTG 1 cut(s) 630
MvaI CCWGG 2 cut(s) 188, 602
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 2 cut(s) 562, 563
NcoI CCATGG 1 cut(s) 540
NdeII GATC 2 cut(s) 506, 694
NlaIII CATG 5 cut(s) 383, 470, 523, 530, 544
NlaIV GGNNCC 3 cut(s) 559, 592, 669
NmuCI GTSAC 6 cut(s) 175, 389, 533, 576, 688, 749
PdmI GAANNNNTTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 600
PinAI ACCGGT 1 cut(s) 753
PkrI GCNGC 2 cut(s) 193, 373
PleI GAGTC 2 cut(s) 18, 676
PmaCI CACGTG 1 cut(s) 363
PmlI CACGTG 1 cut(s) 363
PpsI GAGTC 2 cut(s) 18, 676
Ppu21I YACGTR 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 686
Psp6I CCWGG 2 cut(s) 186, 600
PspCI CACGTG 1 cut(s) 363
PspEI GGTNACC 2 cut(s) 688, 749
PspGI CCWGG 2 cut(s) 186, 600
PspN4I GGNNCC 3 cut(s) 559, 592, 669
PspPI GGNCC 1 cut(s) 667
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 506
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 531
SatI GCNGC 2 cut(s) 192, 372
Sau3AI GATC 2 cut(s) 506, 694
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 2 cut(s) 19, 676
ScrFI CCNGG 4 cut(s) 188, 562, 563, 602
SduI GDGCHC 3 cut(s) 122, 143, 642
SetI ASST 9 cut(s) 35, 62, 202, 299, 307, 365, 515, 578, 639
SfcI CTRYAG 1 cut(s) 192
SmaI CCCGGG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
Sse9I AATT 7 cut(s) 235, 441, 450, 460, 618, 630, 724
SsiI CCGC 1 cut(s) 484
SspI AATATT 1 cut(s) 407
SspMI CTAG 2 cut(s) 429, 510
StyD4I CCNGG 4 cut(s) 186, 560, 561, 600
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 3 cut(s) 202, 365, 578
TaqI TCGA 1 cut(s) 654
TaqII GACCGA 2 cut(s) 410, 737
TasI AATT 7 cut(s) 235, 441, 450, 460, 618, 630, 724
TscAI CASTG 3 cut(s) 324, 352, 683
TseFI GTSAC 6 cut(s) 175, 389, 533, 576, 688, 749
TseI GCWGC 2 cut(s) 191, 371
Tsp45I GTSAC 6 cut(s) 175, 389, 533, 576, 688, 749
TspDTI ATGAA 3 cut(s) 154, 221, 536
TspMI CCCGGG 1 cut(s) 561
TspRI CASTG 3 cut(s) 324, 352, 683
VneI GTGCAC 1 cut(s) 638
XapI RAATTY 3 cut(s) 450, 460, 724
XmaI CCCGGG 1 cut(s) 561
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 2 cut(s) 429, 510
ZraI GACGTC 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.