aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 85 |
| AgsI | TTSAA | 5 cut(s) | 27, 47, 101, 163, 250 |
| AluBI | AGCT | 1 cut(s) | 62 |
| AluI | AGCT | 1 cut(s) | 62 |
| Alw21I | GWGCWC | 1 cut(s) | 64 |
| Alw26I | GTCTC | 1 cut(s) | 173 |
| BanII | GRGCYC | 1 cut(s) | 64 |
| Bbv12I | GWGCWC | 1 cut(s) | 64 |
| BciVI | GTATCC | 1 cut(s) | 161 |
| BcoDI | GTCTC | 1 cut(s) | 173 |
| BfaI | CTAG | 2 cut(s) | 54, 325 |
| BfuI | GTATCC | 1 cut(s) | 161 |
| BpmI | CTGGAG | 1 cut(s) | 103 |
| BsaI | GGTCTC | 1 cut(s) | 173 |
| BseGI | GGATG | 1 cut(s) | 17 |
| BseRI | GAGGAG | 1 cut(s) | 106 |
| BsiHKAI | GWGCWC | 1 cut(s) | 64 |
| BsmAI | GTCTC | 1 cut(s) | 173 |
| Bso31I | GGTCTC | 1 cut(s) | 173 |
| Bsp1286I | GDGCHC | 1 cut(s) | 64 |
| BspQI | GCTCTTC | 1 cut(s) | 52 |
| BspTNI | GGTCTC | 1 cut(s) | 173 |
| Bst6I | CTCTTC | 1 cut(s) | 52 |
| BstC8I | GCNNGC | 1 cut(s) | 6 |
| BstF5I | GGATG | 1 cut(s) | 17 |
| BstMAI | GTCTC | 1 cut(s) | 173 |
| BsuI | GTATCC | 1 cut(s) | 161 |
| BtsCI | GGATG | 1 cut(s) | 17 |
| Cac8I | GCNNGC | 1 cut(s) | 6 |
| Csp6I | GTAC | 1 cut(s) | 84 |
| CviAII | CATG | 1 cut(s) | 314 |
| CviJI | RGCY | 2 cut(s) | 62, 234 |
| CviKI_1 | RGCY | 2 cut(s) | 62, 234 |
| CviQI | GTAC | 1 cut(s) | 84 |
| DraI | TTTAAA | 1 cut(s) | 148 |
| Eam1104I | CTCTTC | 1 cut(s) | 52 |
| EarI | CTCTTC | 1 cut(s) | 52 |
| Ecl136II | GAGCTC | 1 cut(s) | 62 |
| Eco24I | GRGCYC | 1 cut(s) | 64 |
| Eco31I | GGTCTC | 1 cut(s) | 173 |
| Eco53kI | GAGCTC | 1 cut(s) | 62 |
| EcoICRI | GAGCTC | 1 cut(s) | 62 |
| EcoT38I | GRGCYC | 1 cut(s) | 64 |
| FaeI | CATG | 1 cut(s) | 317 |
| FaiI | YATR | 9 cut(s) | 66, 112, 137, 155, 176, 245, 285, 310, 315 |
| FatI | CATG | 1 cut(s) | 313 |
| FokI | GGATG | 1 cut(s) | 4 |
| FriOI | GRGCYC | 1 cut(s) | 64 |
| FspBI | CTAG | 2 cut(s) | 54, 325 |
| GsuI | CTGGAG | 1 cut(s) | 103 |
| Hin1II | CATG | 1 cut(s) | 317 |
| Hpy166II | GTNNAC | 1 cut(s) | 322 |
| Hpy188III | TCNNGA | 3 cut(s) | 54, 101, 120 |
| Hpy8I | GTNNAC | 1 cut(s) | 322 |
| HpyAV | CCTTC | 2 cut(s) | 41, 123 |
| HpyCH4V | TGCA | 2 cut(s) | 35, 213 |
| Hsp92II | CATG | 1 cut(s) | 317 |
| LguI | GCTCTTC | 1 cut(s) | 52 |
| LpnPI | CCDG | 1 cut(s) | 133 |
| MaeI | CTAG | 2 cut(s) | 54, 325 |
| MaeIII | GTNAC | 1 cut(s) | 13 |
| MboII | GAAGA | 1 cut(s) | 69 |
| MhlI | GDGCHC | 1 cut(s) | 64 |
| MluCI | AATT | 3 cut(s) | 42, 89, 300 |
| MnlI | CCTC | 3 cut(s) | 97, 127, 298 |
| MseI | TTAA | 4 cut(s) | 147, 216, 294, 299 |
| MslI | CAYNNNNRTG | 1 cut(s) | 162 |
| NlaIII | CATG | 1 cut(s) | 317 |
| NmuCI | GTSAC | 1 cut(s) | 13 |
| PciSI | GCTCTTC | 1 cut(s) | 52 |
| Psp124BI | GAGCTC | 1 cut(s) | 64 |
| RsaI | GTAC | 1 cut(s) | 85 |
| RsaNI | GTAC | 1 cut(s) | 84 |
| RseI | CAYNNNNRTG | 1 cut(s) | 162 |
| SacI | GAGCTC | 1 cut(s) | 64 |
| SapI | GCTCTTC | 1 cut(s) | 52 |
| SaqAI | TTAA | 4 cut(s) | 147, 216, 294, 299 |
| SduI | GDGCHC | 1 cut(s) | 64 |
| SetI | ASST | 3 cut(s) | 42, 52, 64 |
| SgeI | CNNG | 8 cut(s) | 17, 39, 44, 66, 90, 113, 132, 172 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 162 |
| Sse9I | AATT | 3 cut(s) | 42, 89, 300 |
| SspMI | CTAG | 2 cut(s) | 54, 325 |
| SstI | GAGCTC | 1 cut(s) | 64 |
| TaqI | TCGA | 1 cut(s) | 70 |
| TasI | AATT | 3 cut(s) | 42, 89, 300 |
| Tru1I | TTAA | 4 cut(s) | 147, 216, 294, 299 |
| Tru9I | TTAA | 4 cut(s) | 147, 216, 294, 299 |
| TseFI | GTSAC | 1 cut(s) | 13 |
| Tsp45I | GTSAC | 1 cut(s) | 13 |
| TspDTI | ATGAA | 1 cut(s) | 258 |
| XbaI | TCTAGA | 1 cut(s) | 53 |
| XspI | CTAG | 2 cut(s) | 54, 325 |