Rroxscaffold_1G00042140

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002041
Physical Location & Seq
Forward (+)
60163539 .. 60181397
17859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00042140.1

Sequence Viewer

Length: 1359 bp
ATGATGATTGGATCATCACAAGTAATAAAAGGGCGAGGAACTGCCAAATTTTTGCTGCATAATGGCACCACATTTAAAGTCACGACGCTCTATATGCACCAAGAGCTAATCGAACCTTCACTACAACAGAAAGTCTTTTTAGCGACCAAGTTTTTTATGAGGAATTTATTTTCCTCAGTAAATGTCACTTTTTACGAGGAATATGTTGATTCCTCACTAAATGTCATTAGGTTTTCTGCGAGAAACATATTTTCCTCACTATACACCACTTTTTACGACGAATATTTCAGTTCATCACTAAAGGGTCATAAGAACGCGCACCCTGGTCACCTCCCTCACTTCGCCTTCAGCAAAACACACAATTGCAGCTCTCATATCTGTTCCTCCACCACCGCCGCCAACAACACGGTCAAGTCACCGGAGTTTAACGAGAGACTCGGATCTCCATCTCCGACCATGGATTCCGAGCCTTTCGAGAGAGAAAAGAACGTGACTTTTCCGGGGATGATGTACCAGCTCCTCCCACACCAATCCGAAAAAGCTTCATCATCTTCTTCTTCTTCTTCCTTTGAAGGTCCTCGTTCTACCGAGGGCCTCTCTGCCGCTTTGTCCGATCGATCGGCCGAAGATGGCGATGAGCGTGAGAGGAGAGATGGAGAAGGCCGAGCGTGGAGCGAGTTGAAGGCGGTGAAAGAGCAAACCGATCCGGAGGTCAATCTTCTCCGGGACTCTCTCAACAACATCCGCACCGCCACCACCCGCCTCGAGCTCGCCTCCTCCGCCCTCCACGACCTCTCCCTCCGACCCACGGGCAAAAAGATGCTCGTCCCTCTTACCGCCTCCCTTTACGTCCCCGGAACTCTCCAGGCTACTTCATCGAGAAGAACATGCCTCAAGCCAAAGACTACTGCGACCGCAAGATCAATCTCCTCAAGTCCAATTTCGAACAACTCGTTGAGGTTAGACGACAATTCGATCCTCAAATTGTTGATAGAAGACGATGCCCTAAAATTAGTCGGTGTCATCACCAGGCCTCCACCATCTAGCACTTCTCGATCGTCAATAGATGAAACGTCTAACAAGAATAAAGAGTTAGAAGATAAACTTGAAGAGACCCAAGATACTATAAAGGCTCAACAAGAAAAGATTGATGCACAAAACGTGTTGATCCAAGAATTGCAGGAGCAAGGCAAAAAGTTTGAGCAGTTCATGGCAACCTTTATGAACCAACAAGCATCAATTGAAAAACTTGATGCCTCTAAAATTTTAAAATATTCAACATATGCTTTTTTGGAGAGCATGGAAGGGTGGCCTCCTGGCAAGATACCGGCCGACCTCCACCATGGCAAGGTAGCTTAG

Protein Analysis

452

Amino Acids

50.34

Weight (kDa)

6.88

Isoelectric Point (pI)

60.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 236 - 292 2.8e-07 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 317
AccIII TCCGGA 1 cut(s) 706
AclWI GGATC 5 cut(s) 19, 448, 698, 970, 1162
AcoI YGGCCR 2 cut(s) 621, 1329
AcsI RAATTY 3 cut(s) 47, 163, 1263
AcuI CTGAAG 1 cut(s) 331
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 808, 1348
AflIII ACRYGT 1 cut(s) 1161
AgsI TTSAA 5 cut(s) 572, 682, 1109, 1244, 1278
AjnI CCWGG 4 cut(s) 322, 864, 1028, 1315
AluBI AGCT 6 cut(s) 106, 369, 517, 542, 769, 1355
AluI AGCT 6 cut(s) 106, 369, 517, 542, 769, 1355
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 2 cut(s) 427, 1106
AlwI GGATC 5 cut(s) 19, 448, 698, 970, 1162
Ama87I CYCGRG 1 cut(s) 764
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 6 cut(s) 592, 621, 661, 1031, 1310, 1329
ApeKI GCWGC 2 cut(s) 55, 366
ApoI RAATTY 3 cut(s) 47, 163, 1263
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 2 cut(s) 575, 592
AsuC2I CCSGG 3 cut(s) 501, 725, 855
AsuHPI GGTGA 4 cut(s) 320, 408, 700, 1018
AsuII TTCGAA 1 cut(s) 944
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 771
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 2 cut(s) 42, 378
BccI CCATC 4 cut(s) 454, 623, 647, 1048
BciT130I CCWGG 4 cut(s) 324, 866, 1030, 1317
BcnI CCSGG 3 cut(s) 501, 725, 855
BcoDI GTCTC 2 cut(s) 427, 1106
BfaI CTAG 1 cut(s) 1044
BisI GCNGC 4 cut(s) 56, 367, 396, 603
BlsI GCNGC 4 cut(s) 57, 368, 397, 604
Bme1390I CCNGG 7 cut(s) 324, 501, 725, 855, 866, 1030, 1317
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 2 cut(s) 575, 592
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 7 cut(s) 324, 501, 725, 855, 866, 1030, 1317
BmsI GCATC 5 cut(s) 810, 991, 1141, 1243, 1244
BpiI GAAGAC 1 cut(s) 1002
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 758, 790
BpmI CTGGAG 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 944
BpuEI CTTGAG 2 cut(s) 878, 916
BpuMI CCSGG 3 cut(s) 501, 725, 855
Bsa29I ATCGAT 1 cut(s) 616
BsaBI GATNNNNATC 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 7 cut(s) 322, 456, 500, 588, 807, 853, 1342
BsaWI WCCGGW 2 cut(s) 418, 706
BsaXI ACNNNNNCTCC 4 cut(s) 779, 809, 1018, 1048
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1348
Bse118I RCCGGY 1 cut(s) 1327
Bse8I GATNNNNATC 1 cut(s) 445
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 4 cut(s) 324, 866, 1030, 1317
BseCI ATCGAT 1 cut(s) 616
BseDI CCNNGG 7 cut(s) 322, 456, 500, 588, 807, 853, 1342
BseGI GGATG 2 cut(s) 510, 741
BseJI GATNNNNATC 1 cut(s) 445
BseLI CCNNNNNNNGG 2 cut(s) 808, 1348
BseMII CTCAG 1 cut(s) 189
BseRI GAGGAG 4 cut(s) 509, 661, 766, 919
BseX3I CGGCCG 2 cut(s) 621, 1329
BseXI GCAGC 2 cut(s) 42, 378
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 6 cut(s) 616, 620, 624, 915, 1058, 1332
BshFI GGCC 6 cut(s) 594, 623, 663, 1033, 1312, 1331
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 616
BsiEI CGRYCG 6 cut(s) 616, 620, 624, 915, 1058, 1332
BsiHKAI GWGCWC 1 cut(s) 771
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 6 cut(s) 419, 500, 707, 724, 855, 1328
BslFI GGGAC 3 cut(s) 740, 812, 836
BslI CCNNNNNNNGG 2 cut(s) 808, 1348
BsmAI GTCTC 2 cut(s) 427, 1106
BsmFI GGGAC 3 cut(s) 740, 812, 836
BsnI GGCC 6 cut(s) 594, 623, 663, 1033, 1312, 1331
Bso31I GGTCTC 1 cut(s) 1106
BsoBI CYCGRG 1 cut(s) 764
Bsp119I TTCGAA 1 cut(s) 944
Bsp1286I GDGCHC 1 cut(s) 771
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
Bsp19I CCATGG 2 cut(s) 456, 1342
BspANI GGCC 6 cut(s) 594, 623, 663, 1033, 1312, 1331
BspCNI CTCAG 1 cut(s) 188
BspDI ATCGAT 1 cut(s) 616
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 5 cut(s) 19, 448, 698, 970, 1162
BspT104I TTCGAA 1 cut(s) 944
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 1106
BsrFI RCCGGY 1 cut(s) 1327
BssAI RCCGGY 1 cut(s) 1327
BssECI CCNNGG 7 cut(s) 322, 456, 500, 588, 807, 853, 1342
BssMI GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
BssT1I CCWWGG 2 cut(s) 456, 1342
Bst2UI CCWGG 4 cut(s) 324, 866, 1030, 1317
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 1 cut(s) 1104
BstBI TTCGAA 1 cut(s) 944
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 2 cut(s) 175, 1356
BstDSI CCRYGG 3 cut(s) 456, 807, 1342
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 2 cut(s) 510, 741
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 9 cut(s) 14, 443, 616, 620, 706, 923, 978, 1058, 1170
BstMAI GTCTC 2 cut(s) 427, 1106
BstMBI GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
BstMCI CGRYCG 6 cut(s) 616, 620, 624, 915, 1058, 1332
BstMWI GCNNNNNNNGC 3 cut(s) 94, 103, 779
BstNI CCWGG 4 cut(s) 324, 866, 1030, 1317
BstNSI RCATGY 1 cut(s) 891
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 7 cut(s) 322, 499, 723, 853, 864, 1028, 1315
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 42, 378
BstV2I GAAGAC 1 cut(s) 1002
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
BstZI CGGCCG 2 cut(s) 621, 1329
Bsu15I ATCGAT 1 cut(s) 616
BsuRI GGCC 6 cut(s) 594, 623, 663, 1033, 1312, 1331
BsuTUI ATCGAT 1 cut(s) 616
BtgI CCRYGG 3 cut(s) 456, 807, 1342
BtgZI GCGATG 1 cut(s) 648
BtsCI GGATG 2 cut(s) 510, 741
Cac8I GCNNGC 1 cut(s) 771
CfoI GCGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 1327
Cfr13I GGNCC 2 cut(s) 575, 592
ClaI ATCGAT 1 cut(s) 616
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 511
CviAII CATG 5 cut(s) 457, 888, 1210, 1300, 1343
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 2 cut(s) 175, 1356
DpnI GATC 9 cut(s) 13, 442, 615, 619, 705, 922, 977, 1057, 1169
DpnII GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
DraI TTTAAA 2 cut(s) 76, 1269
EaeI YGGCCR 2 cut(s) 621, 1329
EagI CGGCCG 2 cut(s) 621, 1329
Eam1104I CTCTTC 1 cut(s) 1104
EarI CTCTTC 1 cut(s) 1104
EciI GGCGGA 1 cut(s) 769
Ecl136II GAGCTC 1 cut(s) 769
EclXI CGGCCG 2 cut(s) 621, 1329
Eco130I CCWWGG 2 cut(s) 456, 1342
Eco147I AGGCCT 1 cut(s) 1033
Eco24I GRGCYC 1 cut(s) 771
Eco31I GGTCTC 1 cut(s) 1106
Eco47I GGWCC 1 cut(s) 575
Eco52I CGGCCG 2 cut(s) 621, 1329
Eco53kI GAGCTC 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 764
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 769
EcoO109I RGGNCCY 2 cut(s) 575, 592
EcoO65I GGTNACC 1 cut(s) 326
EcoRII CCWGG 4 cut(s) 322, 864, 1028, 1315
EcoT14I CCWWGG 2 cut(s) 456, 1342
EcoT38I GRGCYC 1 cut(s) 771
ErhI CCWWGG 2 cut(s) 456, 1342
FaeI CATG 5 cut(s) 460, 891, 1213, 1303, 1346
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 3 cut(s) 740, 812, 836
FatI CATG 5 cut(s) 456, 887, 1209, 1299, 1342
FauI CCCGC 1 cut(s) 767
FauNDI CATATG 1 cut(s) 1282
Fnu4HI GCNGC 4 cut(s) 56, 367, 396, 603
FokI GGATG 2 cut(s) 517, 728
FriOI GRGCYC 1 cut(s) 771
Fsp4HI GCNGC 4 cut(s) 56, 367, 396, 603
FspBI CTAG 1 cut(s) 1044
GlaI GCGC 1 cut(s) 318
GluI GCNGC 4 cut(s) 56, 367, 396, 603
GsuI CTGGAG 1 cut(s) 848
HaeIII GGCC 6 cut(s) 594, 623, 663, 1033, 1312, 1331
HapII CCGG 6 cut(s) 419, 500, 707, 724, 855, 1328
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 319
Hin1II CATG 5 cut(s) 460, 891, 1213, 1303, 1346
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 4 cut(s) 209, 435, 461, 728
HpaII CCGG 6 cut(s) 419, 500, 707, 724, 855, 1328
HphI GGTGA 4 cut(s) 320, 408, 700, 1018
Hpy188I TCNGA 6 cut(s) 440, 453, 466, 535, 613, 803
Hpy188III TCNNGA 5 cut(s) 82, 475, 707, 879, 1053
Hpy99I CGWCG 2 cut(s) 88, 281
HpyAV CCTTC 6 cut(s) 126, 355, 566, 653, 676, 1298
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4IV ACGT 4 cut(s) 489, 849, 1073, 1161
HpyCH4V TGCA 5 cut(s) 58, 97, 366, 1154, 1180
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 103, 779
HpyF3I CTNAG 2 cut(s) 175, 1356
HpySE526I ACGT 4 cut(s) 489, 849, 1073, 1161
Hsp92II CATG 5 cut(s) 460, 891, 1213, 1303, 1346
HspAI GCGC 1 cut(s) 317
Kpn2I TCCGGA 1 cut(s) 706
Kzo9I GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
LmnI GCTCC 3 cut(s) 522, 672, 1183
Lsp1109I GCAGC 2 cut(s) 42, 378
LweI GCATC 5 cut(s) 810, 991, 1141, 1243, 1244
MaeI CTAG 1 cut(s) 1044
MaeII ACGT 4 cut(s) 489, 849, 1073, 1161
MaeIII GTNAC 5 cut(s) 79, 184, 326, 414, 490
MalI GATC 9 cut(s) 13, 442, 615, 619, 705, 922, 977, 1057, 1169
MboI GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
MfeI CAATTG 2 cut(s) 361, 1239
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 771
MlyI GAGTC 2 cut(s) 429, 722
MmeI TCCRAC 2 cut(s) 476, 826
MroI TCCGGA 1 cut(s) 706
MseI TTAA 3 cut(s) 75, 426, 1268
MspI CCGG 6 cut(s) 419, 500, 707, 724, 855, 1328
MspR9I CCNGG 7 cut(s) 324, 501, 725, 855, 866, 1030, 1317
MunI CAATTG 2 cut(s) 361, 1239
MvaI CCWGG 4 cut(s) 324, 866, 1030, 1317
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 3 cut(s) 94, 103, 779
NciI CCSGG 3 cut(s) 501, 725, 855
NcoI CCATGG 2 cut(s) 456, 1342
NdeI CATATG 1 cut(s) 1282
NdeII GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
NlaIII CATG 5 cut(s) 460, 891, 1213, 1303, 1346
NlaIV GGNNCC 1 cut(s) 67
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 5 cut(s) 79, 184, 326, 414, 490
NspI RCATGY 1 cut(s) 891
NspV TTCGAA 1 cut(s) 944
PaeR7I CTCGAG 1 cut(s) 764
PceI AGGCCT 1 cut(s) 1033
PcsI WCGNNNNNNNCGW 1 cut(s) 950
PfeI GAWTC 2 cut(s) 209, 461
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 4 cut(s) 57, 368, 397, 604
Ple19I CGATCG 3 cut(s) 616, 620, 1058
PleI GAGTC 2 cut(s) 429, 722
PpsI GAGTC 2 cut(s) 429, 722
PpuMI RGGWCCY 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 4 cut(s) 322, 864, 1028, 1315
PspEI GGTNACC 1 cut(s) 326
PspGI CCWGG 4 cut(s) 322, 864, 1028, 1315
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 575, 592
PspPPI RGGWCCY 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 764
PsrI GAACNNNNNNTAC 2 cut(s) 105, 137
PsuI RGATCY 1 cut(s) 440
PvuI CGATCG 3 cut(s) 616, 620, 1058
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SacI GAGCTC 1 cut(s) 771
SaqAI TTAA 3 cut(s) 75, 426, 1268
SatI GCNGC 4 cut(s) 56, 367, 396, 603
Sau3AI GATC 9 cut(s) 11, 440, 613, 617, 703, 920, 975, 1055, 1167
Sau96I GGNCC 2 cut(s) 575, 592
SchI GAGTC 2 cut(s) 429, 722
ScrFI CCNGG 7 cut(s) 324, 501, 725, 855, 866, 1030, 1317
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 5 cut(s) 810, 991, 1141, 1243, 1244
Sfr274I CTCGAG 1 cut(s) 764
SfuI TTCGAA 1 cut(s) 944
SinI GGWCC 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 764
SmlI CTYRAG 3 cut(s) 764, 893, 931
SmoI CTYRAG 3 cut(s) 764, 893, 931
SseBI AGGCCT 1 cut(s) 1033
SspI AATATT 2 cut(s) 284, 1274
SspMI CTAG 1 cut(s) 1044
SstI GAGCTC 1 cut(s) 771
StuI AGGCCT 1 cut(s) 1033
StyD4I CCNGG 7 cut(s) 322, 499, 723, 853, 864, 1028, 1315
StyI CCWWGG 2 cut(s) 456, 1342
TaaI ACNGT 1 cut(s) 409
TaiI ACGT 4 cut(s) 492, 852, 1076, 1164
TaqI TCGA 8 cut(s) 111, 474, 616, 765, 878, 944, 974, 1054
TauI GCSGC 2 cut(s) 398, 605
TfiI GAWTC 2 cut(s) 209, 461
Tru1I TTAA 3 cut(s) 75, 426, 1268
Tru9I TTAA 3 cut(s) 75, 426, 1268
TseFI GTSAC 5 cut(s) 79, 184, 326, 414, 490
TseI GCWGC 2 cut(s) 55, 366
Tsp45I GTSAC 5 cut(s) 79, 184, 326, 414, 490
TspDTI ATGAA 6 cut(s) 282, 534, 864, 1083, 1198, 1238
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 3 cut(s) 47, 163, 1263
XceI RCATGY 1 cut(s) 891
XhoI CTCGAG 1 cut(s) 764
XspI CTAG 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.