pycom13g23550

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002041
Physical Location & Seq
Forward (+)
21053249 .. 21055371
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23550.2

Sequence Viewer

Length: 1701 bp
ATGGATGAAATAGTGTACCATGAGTCTGATGTTTGTTTTTCACCCAAAAGTACACGTGTCACCTCCATAGGCATGGTAGAGGCAGCCAAAAGCCACCCCGTCAAAGGTAACGAAGCCAAGGCCTACCTAAGGGAGGAAACTGAGCCCAGAAGCGGCAAAGCATCTGAAACCATGGATGCGGACATATGCTATCGCTATGGTTCAAAGGACCATGGGTCCTGTGTTTGCCGAGATCCCCAAAAGGTTGTAGTTGAATATCATTCTTGTTGTAAGAAGTTTGAATCAAACTTTGTGCAAGTGGAAGAACCAGAGAGTACCAAGATAGAGGTTTTTGACTTTCAGGAGGATACCAACCCTAATAATGGGGCCGAATTCCACCTAGTGGCCAACTCATCCCCCCCTTGTTTTTGGTTTAATTTTGAACAATTTCCTTTATGTTTGGATTATTTGTTGGTGATTTGTTTTTGGATATTAATTTTATTTTTAGGTATGACTTGTGGGAAAGTTAGTTGTCTAGCAGATAATGCAACCACGCACATCGTTTTGCGTGAACGTATCTATTTCACTAACTTCATACCTAAGAATACACCTCTGACAACCCTCTCAGGACCATCCAACCTGATCGAAGGGTACAATAAGTCACGTATAATGTTGTCCAATGGTACAATCTTAACCATTGATAAGGCACTTTATTCTCTACATTCTGGAAGAACATTGTTGACCTTCAAGGATATTAGAAATAATAATTACCATACTAAAACCTATGTAGAAAACGGACGTATTCTAGAGAAGATAGAGCGTGTTCTGAGTGGTTTGTATACTACGACCATACACCATATAGAAAGCCACTATGTGGCTGGCCCTACCTCTGGGACCATGCACGAAATTACACTTTGGTATGATAGTTTAGGACACCTTGGACTATCAGCAATATGTCGTATCCTCAAATCATCACATGGGAATCCACTAACCCGAAGTTTAGGTTCGATTCAAGGAATGACATGTCAAACCTATTCCTTCGAATCCTTCTATTTTTCTACAAAGGATTCATGGGACAGTTATGGACCGATTCATCCTCCATGCGGACCATTTAGATATTTTAAGGTTTTGTTTGACGCTTCCACACGTTGGTTGCTTCCACACGTTAGTTGCACGTGTGCTTGTTGTCCACAAAGAACGCTGCATTCTCCAAACTCTAGGCTCAGGTTTTCAAGCTCAAGGCTTACCACCCTAATTATTCGATCAAATCTATTTGATTGGATAATGTCGGTTGAGGTTGAAATTGAACATCCAGTACGCCATGTTCACACCCAGAACAGCGTCGCAGAGGCATTCATTAAGCTCTTACAAATGATTATTCGATCATTGGTCATACGTACCAAGCTCTCAATCGTTGCTTGGGGCCATGCAATATTGCACTCAGGCATGTTGGTCCGCTTGAGGCTTGTTGCAACGCAACCATATAGTGCTCTTCAGGTTACCAGATACAAACCCGACATATCGTATCTGCGCATTTTTGATTATGCGGGCATCAGCGAAGGATGTGAATCTATTTTCGGATATGATTATCTTTCAATCATTCATTGCTTAGAACCTTTGATAGGCAATCTATTTACTGCTTGTTTCGCGAAATGTCATTTTTATGAGACAATCTTCCCGTCGTTAGGGGGAGATAAGAATGTCAACGTTCCTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

567

Amino Acids

64.21

Weight (kDa)

6.77

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1511
AccB7I CCANNNNNTGG 3 cut(s) 382, 853, 1128
AccI GTMKAC 1 cut(s) 818
AccII CGCG 1 cut(s) 1628
AciI CCGC 5 cut(s) 153, 179, 1083, 1435, 1526
AclI AACGTT 1 cut(s) 1686
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 1457
AcvI CACGTG 2 cut(s) 56, 1155
AdeI CACNNNGTG 2 cut(s) 382, 853
AfaI GTAC 7 cut(s) 17, 52, 316, 632, 664, 1296, 1378
AflIII ACRYGT 6 cut(s) 53, 55, 1001, 1124, 1141, 1154
AhdI GACNNNNNGTC 1 cut(s) 214
AluBI AGCT 3 cut(s) 1215, 1342, 1384
AluI AGCT 3 cut(s) 1215, 1342, 1384
Alw21I GWGCWC 1 cut(s) 1471
Alw26I GTCTC 1 cut(s) 1640
AlwI GGATC 1 cut(s) 227
AoxI GGCC 5 cut(s) 120, 366, 384, 859, 1402
ApeKI GCWGC 2 cut(s) 83, 1180
ApoI RAATTY 1 cut(s) 371
AseI ATTAAT 1 cut(s) 473
Asp700I GAANNNNTTC 1 cut(s) 426
AspLEI GCGC 1 cut(s) 1512
AsuHPI GGTGA 3 cut(s) 33, 52, 466
AsuII TTCGAA 1 cut(s) 1020
AvaII GGWCC 7 cut(s) 208, 216, 608, 873, 1064, 1085, 1432
AxyI CCTNAGG 1 cut(s) 128
BalI TGGCCA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 147
BbrPI CACGTG 2 cut(s) 56, 1155
Bbv12I GWGCWC 1 cut(s) 1471
BbvI GCAGC 2 cut(s) 95, 1167
BccI CCATC 1 cut(s) 619
BciVI GTATCC 2 cut(s) 340, 950
BcoDI GTCTC 1 cut(s) 1640
BfaI CTAG 4 cut(s) 380, 515, 785, 1197
BfuI GTATCC 2 cut(s) 340, 950
BisI GCNGC 3 cut(s) 84, 154, 1181
BlsI GCNGC 3 cut(s) 85, 155, 1182
Bme18I GGWCC 7 cut(s) 208, 216, 608, 873, 1064, 1085, 1432
BmeRI GACNNNNNGTC 1 cut(s) 214
BmiI GGNNCC 4 cut(s) 217, 367, 874, 1403
BmsI GCATC 3 cut(s) 166, 170, 1539
Bpu10I CCTNAGC 1 cut(s) 1202
Bpu14I TTCGAA 1 cut(s) 1020
BpuEI CTTGAG 2 cut(s) 1201, 1459
BsaAI YACGTR 4 cut(s) 56, 644, 1155, 1376
BsaBI GATNNNNATC 1 cut(s) 1546
BsaJI CCNNGG 4 cut(s) 117, 171, 211, 916
Bse1I ACTGG 1 cut(s) 1292
Bse21I CCTNAGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 1582
Bse8I GATNNNNATC 1 cut(s) 1546
BseDI CCNNGG 4 cut(s) 117, 171, 211, 916
BseGI GGATG 7 cut(s) 10, 181, 392, 611, 1072, 1288, 1547
BseJI GATNNNNATC 1 cut(s) 1546
BseMI GCAATG 1 cut(s) 1582
BseMII CTCAG 5 cut(s) 132, 618, 797, 1216, 1434
BseNI ACTGG 1 cut(s) 1292
BseXI GCAGC 2 cut(s) 95, 1167
Bsh1236I CGCG 1 cut(s) 1628
BshFI GGCC 5 cut(s) 122, 368, 386, 861, 1404
BsiHKAI GWGCWC 1 cut(s) 1471
BslFI GGGAC 2 cut(s) 886, 1067
BsmAI GTCTC 1 cut(s) 1640
BsmFI GGGAC 2 cut(s) 886, 1067
BsmI GAATGC 2 cut(s) 1183, 1331
BsnI GGCC 5 cut(s) 122, 368, 386, 861, 1404
Bsp119I TTCGAA 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 147, 1471
Bsp143I GATC 4 cut(s) 232, 621, 1241, 1361
Bsp19I CCATGG 2 cut(s) 171, 211
Bsp68I TCGCGA 1 cut(s) 1628
BspACI CCGC 5 cut(s) 153, 179, 1083, 1435, 1526
BspANI GGCC 5 cut(s) 122, 368, 386, 861, 1404
BspCNI CTCAG 5 cut(s) 133, 617, 798, 1215, 1433
BspFNI CGCG 1 cut(s) 1628
BspLI GGNNCC 4 cut(s) 217, 367, 874, 1403
BspPI GGATC 1 cut(s) 227
BspQI GCTCTTC 1 cut(s) 1476
BspT104I TTCGAA 1 cut(s) 1020
BsrDI GCAATG 1 cut(s) 1582
BsrI ACTGG 1 cut(s) 1292
BssECI CCNNGG 4 cut(s) 117, 171, 211, 916
BssMI GATC 4 cut(s) 232, 621, 1241, 1361
BssNAI GTATAC 1 cut(s) 819
BssT1I CCWWGG 4 cut(s) 117, 171, 211, 916
Bst1107I GTATAC 1 cut(s) 819
Bst4CI ACNGT 1 cut(s) 1058
Bst6I CTCTTC 1 cut(s) 1476
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBAI YACGTR 4 cut(s) 56, 644, 1155, 1376
BstBI TTCGAA 1 cut(s) 1020
BstC8I GCNNGC 2 cut(s) 859, 1528
BstDEI CTNAG 8 cut(s) 128, 141, 579, 604, 806, 1202, 1420, 1588
BstDSI CCRYGG 2 cut(s) 171, 211
BstEII GGTNACC 1 cut(s) 1477
BstENI CCTNNNNNAGG 3 cut(s) 127, 131, 1599
BstF5I GGATG 7 cut(s) 10, 181, 392, 611, 1072, 1288, 1547
BstFNI CGCG 1 cut(s) 1628
BstHHI GCGC 1 cut(s) 1512
BstKTI GATC 4 cut(s) 235, 624, 1244, 1364
BstMAI GTCTC 1 cut(s) 1640
BstMBI GATC 4 cut(s) 232, 621, 1241, 1361
BstMWI GCNNNNNNNGC 2 cut(s) 524, 1625
BstNSI RCATGY 2 cut(s) 1005, 1429
BstPI GGTNACC 1 cut(s) 1477
BstSNI TACGTA 1 cut(s) 1376
BstUI CGCG 1 cut(s) 1628
BstV1I GCAGC 2 cut(s) 95, 1167
BstX2I RGATCY 1 cut(s) 232
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 1 cut(s) 232
BstZ17I GTATAC 1 cut(s) 819
Bsu36I CCTNAGG 1 cut(s) 128
BsuI GTATCC 2 cut(s) 340, 950
BsuRI GGCC 5 cut(s) 122, 368, 386, 861, 1404
BtgI CCRYGG 2 cut(s) 171, 211
BtsCI GGATG 7 cut(s) 10, 181, 392, 611, 1072, 1288, 1547
BtuMI TCGCGA 1 cut(s) 1628
Cac8I GCNNGC 2 cut(s) 859, 1528
CfoI GCGC 1 cut(s) 1512
CseI GACGC 2 cut(s) 1124, 1309
Csp6I GTAC 7 cut(s) 16, 51, 315, 631, 663, 1295, 1377
CviQI GTAC 7 cut(s) 16, 51, 315, 631, 663, 1295, 1377
DdeI CTNAG 8 cut(s) 128, 141, 579, 604, 806, 1202, 1420, 1588
DpnI GATC 4 cut(s) 234, 623, 1243, 1363
DpnII GATC 4 cut(s) 232, 621, 1241, 1361
DraIII CACNNNGTG 2 cut(s) 382, 853
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 1476
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 1476
Eco105I TACGTA 1 cut(s) 1376
Eco130I CCWWGG 4 cut(s) 117, 171, 211, 916
Eco147I AGGCCT 1 cut(s) 122
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 7 cut(s) 208, 216, 608, 873, 1064, 1085, 1432
Eco57I CTGAAG 1 cut(s) 1457
Eco72I CACGTG 2 cut(s) 56, 1155
Eco81I CCTNAGG 1 cut(s) 128
Eco91I GGTNACC 1 cut(s) 1477
EcoNI CCTNNNNNAGG 3 cut(s) 127, 131, 1599
EcoO109I RGGNCCY 1 cut(s) 216
EcoO65I GGTNACC 1 cut(s) 1477
EcoRI GAATTC 1 cut(s) 371
EcoT14I CCWWGG 4 cut(s) 117, 171, 211, 916
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 4 cut(s) 117, 171, 211, 916
FaqI GGGAC 2 cut(s) 886, 1067
FauI CCCGC 1 cut(s) 1519
FauNDI CATATG 1 cut(s) 185
FblI GTMKAC 1 cut(s) 818
Fnu4HI GCNGC 3 cut(s) 84, 154, 1181
FokI GGATG 7 cut(s) 17, 188, 379, 598, 1059, 1275, 1554
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 3 cut(s) 84, 154, 1181
FspBI CTAG 4 cut(s) 380, 515, 785, 1197
FspI TGCGCA 1 cut(s) 1511
GlaI GCGC 1 cut(s) 1511
GluI GCNGC 3 cut(s) 84, 154, 1181
HaeIII GGCC 5 cut(s) 122, 368, 386, 861, 1404
HgaI GACGC 2 cut(s) 1124, 1309
HhaI GCGC 1 cut(s) 1512
Hin6I GCGC 1 cut(s) 1510
HinP1I GCGC 1 cut(s) 1510
HincII GTYRAC 2 cut(s) 720, 1684
HindII GTYRAC 2 cut(s) 720, 1684
HinfI GANTC 8 cut(s) 23, 281, 961, 988, 1022, 1046, 1069, 1547
HphI GGTGA 3 cut(s) 33, 52, 466
Hpy166II GTNNAC 8 cut(s) 16, 53, 551, 720, 819, 1169, 1306, 1684
Hpy188I TCNGA 5 cut(s) 28, 166, 594, 807, 1559
Hpy188III TCNNGA 6 cut(s) 341, 606, 705, 785, 1627, 1691
Hpy8I GTNNAC 8 cut(s) 16, 53, 551, 720, 819, 1169, 1306, 1684
Hpy99I CGWCG 2 cut(s) 1325, 1663
HpyAV CCTTC 5 cut(s) 620, 733, 1027, 1036, 1532
HpyCH4III ACNGT 1 cut(s) 1058
HpyCH4IV ACGT 9 cut(s) 55, 553, 643, 778, 1126, 1143, 1154, 1375, 1686
HpyCH4V TGCA 8 cut(s) 295, 527, 880, 1152, 1183, 1409, 1417, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 1625
HpyF3I CTNAG 8 cut(s) 128, 141, 579, 604, 806, 1202, 1420, 1588
HpySE526I ACGT 9 cut(s) 55, 553, 643, 778, 1126, 1143, 1154, 1375, 1686
HspAI GCGC 1 cut(s) 1510
Kzo9I GATC 4 cut(s) 232, 621, 1241, 1361
LguI GCTCTTC 1 cut(s) 1476
Lsp1109I GCAGC 2 cut(s) 95, 1167
LweI GCATC 3 cut(s) 166, 170, 1539
MaeI CTAG 4 cut(s) 380, 515, 785, 1197
MaeII ACGT 9 cut(s) 55, 553, 643, 778, 1126, 1143, 1154, 1375, 1686
MaeIII GTNAC 4 cut(s) 58, 107, 639, 1477
MalI GATC 4 cut(s) 234, 623, 1243, 1363
MboI GATC 4 cut(s) 232, 621, 1241, 1361
MboII GAAGA 5 cut(s) 314, 720, 802, 1463, 1645
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 147, 1471
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 8 cut(s) 371, 415, 425, 474, 745, 885, 1233, 1281
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 639
Mox20I TGGCCA 1 cut(s) 386
MroXI GAANNNNTTC 1 cut(s) 426
MscI TGGCCA 1 cut(s) 386
MseI TTAA 5 cut(s) 414, 473, 671, 1101, 1338
MslI CAYNNNNRTG 2 cut(s) 71, 1641
Msp20I TGGCCA 1 cut(s) 386
Mva1269I GAATGC 2 cut(s) 1183, 1331
MvnI CGCG 1 cut(s) 1628
MwoI GCNNNNNNNGC 2 cut(s) 524, 1625
NcoI CCATGG 2 cut(s) 171, 211
NdeI CATATG 1 cut(s) 185
NdeII GATC 4 cut(s) 232, 621, 1241, 1361
NlaIV GGNNCC 4 cut(s) 217, 367, 874, 1403
NmeAIII GCCGAG 1 cut(s) 254
NmuCI GTSAC 2 cut(s) 58, 639
NruI TCGCGA 1 cut(s) 1628
NsbI TGCGCA 1 cut(s) 1511
NspI RCATGY 2 cut(s) 1005, 1429
NspV TTCGAA 1 cut(s) 1020
PceI AGGCCT 1 cut(s) 122
PciI ACATGT 1 cut(s) 1001
PciSI GCTCTTC 1 cut(s) 1476
PctI GAATGC 2 cut(s) 1183, 1331
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 7 cut(s) 281, 961, 988, 1022, 1046, 1069, 1547
PflMI CCANNNNNTGG 3 cut(s) 382, 853, 1128
PkrI GCNGC 3 cut(s) 85, 155, 1182
PleI GAGTC 1 cut(s) 31
PmaCI CACGTG 2 cut(s) 56, 1155
PmlI CACGTG 2 cut(s) 56, 1155
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 4 cut(s) 56, 644, 1155, 1376
PpuMI RGGWCCY 1 cut(s) 216
PscI ACATGT 1 cut(s) 1001
PshBI ATTAAT 1 cut(s) 473
Psp1406I AACGTT 1 cut(s) 1686
Psp5II RGGWCCY 1 cut(s) 216
PspCI CACGTG 2 cut(s) 56, 1155
PspEI GGTNACC 1 cut(s) 1477
PspN4I GGNNCC 4 cut(s) 217, 367, 874, 1403
PspPPI RGGWCCY 1 cut(s) 216
PsrI GAACNNNNNNTAC 4 cut(s) 1278, 1287, 1310, 1319
PsuI RGATCY 1 cut(s) 232
RruI TCGCGA 1 cut(s) 1628
RsaI GTAC 7 cut(s) 17, 52, 316, 632, 664, 1296, 1378
RsaNI GTAC 7 cut(s) 16, 51, 315, 631, 663, 1295, 1377
RseI CAYNNNNRTG 2 cut(s) 71, 1641
SapI GCTCTTC 1 cut(s) 1476
SaqAI TTAA 5 cut(s) 414, 473, 671, 1101, 1338
SatI GCNGC 3 cut(s) 84, 154, 1181
Sau3AI GATC 4 cut(s) 232, 621, 1241, 1361
SchI GAGTC 1 cut(s) 32
SduI GDGCHC 2 cut(s) 147, 1471
SfaNI GCATC 3 cut(s) 166, 170, 1539
SfuI TTCGAA 1 cut(s) 1020
SinI GGWCC 7 cut(s) 208, 216, 608, 873, 1064, 1085, 1432
SmiMI CAYNNNNRTG 2 cut(s) 71, 1641
SmlI CTYRAG 2 cut(s) 1216, 1438
SmoI CTYRAG 2 cut(s) 1216, 1438
SnaBI TACGTA 1 cut(s) 1376
Sse9I AATT 8 cut(s) 371, 415, 425, 474, 745, 885, 1233, 1281
SseBI AGGCCT 1 cut(s) 122
SsiI CCGC 5 cut(s) 153, 179, 1083, 1435, 1526
SspI AATATT 1 cut(s) 1413
SspMI CTAG 4 cut(s) 380, 515, 785, 1197
StuI AGGCCT 1 cut(s) 122
StyI CCWWGG 4 cut(s) 117, 171, 211, 916
TaaI ACNGT 1 cut(s) 1058
TaiI ACGT 9 cut(s) 58, 556, 646, 781, 1129, 1146, 1157, 1378, 1689
TaqI TCGA 5 cut(s) 624, 986, 1020, 1240, 1360
TaqII GACCGA 1 cut(s) 1081
TasI AATT 8 cut(s) 371, 415, 425, 474, 745, 885, 1233, 1281
TatI WGTACW 1 cut(s) 50
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 7 cut(s) 281, 961, 988, 1022, 1046, 1069, 1547
Tru1I TTAA 5 cut(s) 414, 473, 671, 1101, 1338
Tru9I TTAA 5 cut(s) 414, 473, 671, 1101, 1338
TseFI GTSAC 2 cut(s) 58, 639
TseI GCWGC 2 cut(s) 83, 1180
Tsp45I GTSAC 2 cut(s) 58, 639
TspDTI ATGAA 6 cut(s) 21, 562, 1038, 1061, 1324, 1571
TspGWI ACGGA 1 cut(s) 789
Van91I CCANNNNNTGG 3 cut(s) 382, 853, 1128
VpaK11BI GGWCC 7 cut(s) 208, 216, 608, 873, 1064, 1085, 1432
VspI ATTAAT 1 cut(s) 473
XagI CCTNNNNNAGG 3 cut(s) 127, 131, 1599
XapI RAATTY 1 cut(s) 371
XbaI TCTAGA 1 cut(s) 784
XceI RCATGY 2 cut(s) 1005, 1429
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 4 cut(s) 380, 515, 785, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.