FvH4_7g15611

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002041
Physical Location & Seq
Forward (+)
13476255 .. 13480620
4366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15611.t1

Sequence Viewer

Length: 3255 bp
ATGGACAGAGGCATTGAATTTGACGTCCTCGACGCCCATGGTACTAAGTACCATCGTTGGGTCTCTGACATAGAACAGACCTTCATCGCTAAGGATCTGACCGAGACCATATTCCCCGATCCAGATCAGGAACCGCCTAGCAAGAGAACAAAATCTCAGGCACTCATGTTCCTTCGTAAGCATATCGATCCCACACTGCGCAGACAGTATCAATCCAAACACGATCCAAAAGATCTGTGGGATGCCCTTGCCGAACGCTTCGGCAACATTCACTCGACCTTGCTCCCAGAACTAATTGCTCGCTGGGATGAAATCCCCTTACTTTTGGATTACAAGAAGGTTGATGACTTCAACAGGGATATGCTTTGCCTACAAGCTCAACTGAGCTCATGTGGAGTCGAGAAAAGTGATGCCGACATCGAAAAGACTTTCTCGACCTTCCCTTCAGCTGCTAAGATCCTCATGAACCAATATCGGCTTGAGTTCACAAATAAGAGGATTACTACATTTAGCGGCTTAATGACGCAACTCCTTATGGAAGAAAAGAATAATATGATCAATGATCAGCATAATTTGCGTCCTGTAGGCACCCGGAAAATTCCGGAATCTAATTACAATTGCAACCGGAATAAGAAAGCTCCGAAACGCAAGGATCAACATAGGAATGAACCTTATGCACGTGGGAATCAACACCACCGTGCCTCTAGTTCTCGGGACAAGGTAGCGGCTTTAGTGGCCGTACCAACTCATGGCGTCGAGACACCGGTGCCGCCGGCCCCAAGGGCGGCGCCGCTCCTCCTCGGAAGCAGCATGCTCGCTCTTCTTCTGCCTTTGATGGTCAATGCAACAGATGTGGGTCCAAGGACCACTGGTATGTCTCTTGGAGAGATCGAGTGCCTCGCAGATAATGGATCAACCCACACAATTCTAAGGAACCAGCAACTATTTCTTGAGTTAGTGCCTTGTAAATCATCTGTGACGACAATGATTGGATCTTCCCCTGTGATCCTCGGGCGTGGAACCGCTCGCTTTTTTCTGCCTCATGGCACTATGATCCACGTCACGGACGCTCTTTATGCCGCAAAAGGGCACCGAACCCTGTTGAGCTTTAAGGACATTCGCGCTAATGGCTTTCACTTGGAAACTCATTGTGAAGATGGAACTGAGTTCTTGTGTATCACTTCTCATGAATACGGACGCAAACGTATATTGGAGAAACTCATGAGTCAATCTAGTGGTCTTTACCTTACTACGATTCGGATTATTGAATCCCATGTAGTCGCCAAACAGGATCTTTGGGACTCTGACTCATACAAGCTTTGGCATGATCGACTTGGTCATCCTGGAAGGGACATGATGATCCGAATTCTGCGTAATTCTCATAGACATCCCTTCTTCAAATCAAAACGAAAATATCTGGGAAATGATATCATTTTGAATCATGGTGGTGACACCTCAACTGGTCACCACGGCCTAAAGGGGACTTTGACGTCTCCCGGGCTCGGCACCAACTTCATGGCTGCCGCTCGGCCGCCCCCTCAAACAATTGAGGCGCGCCGTTCTTCCCTTGATGTGTCGTTGACTCTTTCAAACGCTCATCGTTCGTTTTGCAAAGCTTGCTCTTTAGCGAAAGTAGTATCGAGACCGTCTTACGCTAAAGATAACACCGAGAACATTCCATTTCTACATCGTATCCAAGGTGATATTTGTGGACCCATCCATCCTGAATGTGGACCATTTCGATACTTTATGGTCCTGGTGGATGCTTCAACGCGTTGGACTCATGTCGCGCTCTTGTCCACACGTAATGCTGCATTTCTAAAACTCCTAGCACAAATTATTAAACTTCGGGCTCACCACCCGGATTATCCAATTAAGTCAATTCGACTGGATAACGCTGGAGAATTTACATCAAAGACTTTTGATGACTATTGCATGTCTATCGGGATTGCAGTCGAGCATCCCGTACCCCATGTTCACACGCAAAACGGTCTCGCCGAAGCTACGATTAAGCGGCTTCAGCTTATTTCTCGAGCGTTGGTAATGCGCACCAACCTTCCAATTGCGTCATGGGGCTATGCAATATTGCATGCAGCCTTACTTATTCGTTTCAGACCCACTGCCAAACAACCATTCAGTGTGTACCAGTTGGTCACTGGATATGAGCCTGACATTTCGCATCTACGCATTTTCGGTTGTTCGGTTTATGTGCCAATTACGCCTCCACTGCGTTCTAAAATGGGTCCTCAGAGACGATTGGGTATCTACGTTGGTTATGAATCTCCAACGATTATCAGATATCTCGAGCCATTAACAGGTGATCTCTTTACCGCAAGGTTTGCGGATTGTCACTTTGATGAGACACTCTTCCCATCGTTAGGGGGAGATACGAACAAACGTGTTCATGTGGAACGACAGGAATTGTCGTGGTTTGTCCCCACTCTGTCTCATTATGATTCCCACACCTCACAATCTGAACGTGAAGTGAAACATATTCTCGAACTTCAGAAAGTAGCAGATACGATGCCTGACTCTTTCTCCGAGATTGCGAAAGTGACAAGATCAGATATACCAGCTGCAAATGTTCCTGCTAGGCTCGAAGTCCCTAGAAAGGGCACGGCCATCCCCGGGCTCGGTACTGCCGCCGTCGACGGCGCCGCCCATGGTGGAGGGATGGAGGCCGTGGCTCCCCAAAGGAAAAGGGGGAGGCCACCTGGTTCGGTTGACATGCGTCCGAGAAAGAAACGGACCGACAAGACACGAGTCAATCCTTTGATCATCGATGTTGAGAATCCTTCTCACGAGATCATCTCTGATTACAGTTATGTCCACGAGTTGATATTAGAATCCATGGGGGACACCTCAGGGTCAATGTCAACATCAGAGAACATAGAGATCTCCATGTGTTATGATAACACTTATGAATTGATGAACCGGTCATCAACCCATGTCGATGATATATTTGCTTATTCCGTGGCTCATGAAATCATTGAGCACGATGACATCGAACCACGCTCTGTTGCAGAATGCCAAAACAGAGCAGATTGGCCTAAATGGAAAGTTGCAATCCAGGCAGAATTAGACTCTCTAACAAAGAGACAGGTCTTCGGCCCAGTAGTGCTAACTCCGCCGAGTGTAAAGCCTGTTGGACATAAATGGGTCTTTGTTAGAAAGCGTAATGAGAAGAATGAGGTACTTCGGTATAAGGCCCGCCTTGTGGCGCAAGGTTTCTCACAACGCCCTGGGATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

1085

Amino Acids

122.06

Weight (kDa)

9.0

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 413 - 465 1.4e-06 GAG-pre-integrase domain
rve PF00665 564 - 654 2.4e-09 Integrase core domain
RVT_2 PF07727 1039 - 1084 4.5e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1489
AatII GACGTC 2 cut(s) 27, 1493
Acc16I TGCGCA 2 cut(s) 200, 2048
AccB1I GGYRCC 6 cut(s) 587, 766, 787, 1089, 1505, 2654
AccB7I CCANNNNNTGG 1 cut(s) 749
AccBSI CCGCTC 3 cut(s) 793, 1025, 1526
AccI GTMKAC 1 cut(s) 2649
AccII CGCG 4 cut(s) 1122, 1555, 1774, 1790
AccIII TCCGGA 1 cut(s) 601
AcoI YGGCCR 3 cut(s) 735, 1529, 2619
AcsI RAATTY 4 cut(s) 17, 597, 1365, 1904
AcuI CTGAAG 3 cut(s) 429, 2003, 2489
AcvI CACGTG 1 cut(s) 680
AcyI GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
AfaI GTAC 7 cut(s) 43, 50, 741, 1968, 2144, 2638, 3195
AflIII ACRYGT 3 cut(s) 1772, 1802, 2398
AgeI ACCGGT 2 cut(s) 763, 2934
AgsI TTSAA 7 cut(s) 17, 352, 1268, 1399, 1438, 1590, 1770
AjiI CACGTC 1 cut(s) 1060
AjnI CCWGG 5 cut(s) 1342, 1755, 2713, 3069, 3241
AjuI GAANNNNNNNTTGG 2 cut(s) 2117, 2149
AleI CACNNNNGTG 1 cut(s) 696
Alw21I GWGCWC 2 cut(s) 389, 2997
Ama87I CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
Aor13HI TCCGGA 1 cut(s) 601
ApeKI GCWGC 6 cut(s) 449, 807, 1520, 1811, 2093, 2576
ApoI RAATTY 4 cut(s) 17, 597, 1365, 1904
AscI GGCGCGCC 1 cut(s) 1553
AsiGI ACCGGT 2 cut(s) 763, 2934
AspLEI GCGC 9 cut(s) 201, 790, 1124, 1555, 1557, 1792, 2049, 2657, 3223
AsuC2I CCSGG 6 cut(s) 592, 1497, 1498, 1862, 2628, 2629
AsuHPI GGTGA 5 cut(s) 1457, 1460, 1712, 1847, 2330
AvaI CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
AvaII GGWCC 7 cut(s) 857, 864, 1712, 1733, 1753, 2243, 2748
AxyI CCTNAGG 1 cut(s) 2863
BaeGI GKGCMC 2 cut(s) 1092, 2618
BaeI ACNNNNGTAYC 2 cut(s) 3185, 3218
BanI GGYRCC 6 cut(s) 587, 766, 787, 1089, 1505, 2654
BanII GRGCYC 4 cut(s) 389, 1503, 1855, 2634
BauI CACGAG 3 cut(s) 2760, 2801, 2831
BbrPI CACGTG 1 cut(s) 680
BbsI GAAGAC 1 cut(s) 3097
Bbv12I GWGCWC 2 cut(s) 389, 2997
BbvI GCAGC 6 cut(s) 436, 819, 1507, 1798, 2105, 2563
BccI CCATC 8 cut(s) 60, 829, 1151, 1724, 1728, 2380, 2630, 2668
BceAI ACGGC 7 cut(s) 722, 1486, 1542, 2630, 2634, 2666, 2668
BcgI CGANNNNNNTGC 4 cut(s) 796, 830, 1924, 1958
BciT130I CCWGG 5 cut(s) 1344, 1757, 2715, 3071, 3243
BciVI GTATCC 1 cut(s) 1703
BclI TGATCA 3 cut(s) 555, 562, 2775
BcnI CCSGG 6 cut(s) 592, 1497, 1498, 1862, 2628, 2629
BfaI CTAG 6 cut(s) 138, 705, 1233, 1829, 2592, 2607
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 2 cut(s) 791, 2658
BfuI GTATCC 1 cut(s) 1703
BglI GCCNNNNNGGC 1 cut(s) 782
BglII AGATCT 2 cut(s) 232, 2895
Bme18I GGWCC 7 cut(s) 857, 864, 1712, 1733, 1753, 2243, 2748
BmeT110I CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
BmgBI CACGTC 1 cut(s) 1060
BmrI ACTGGG 1 cut(s) 3107
BmsI GCATC 6 cut(s) 232, 400, 1753, 1969, 2188, 2514
BmuI ACTGGG 1 cut(s) 3107
BoxI GACNNNNGTC 3 cut(s) 1784, 2730, 2762
BpiI GAAGAC 1 cut(s) 3097
BplI GAGNNNNNCTC 2 cut(s) 2795, 2827
BpmI CTGGAG 1 cut(s) 1920
Bpu10I CCTNAGC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 500, 971
BpuMI CCSGG 6 cut(s) 592, 1497, 1498, 1862, 2628, 2629
Bsa29I ATCGAT 2 cut(s) 186, 2781
BsaAI YACGTR 2 cut(s) 680, 1805
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
BsaI GGTCTC 4 cut(s) 67, 98, 1636, 1997
BsaWI WCCGGW 4 cut(s) 601, 624, 763, 2934
BsaXI ACNNNNNCTCC 2 cut(s) 2522, 2552
Bse118I RCCGGY 3 cut(s) 763, 772, 2934
Bse1I ACTGG 6 cut(s) 874, 1465, 1893, 2146, 2161, 3113
Bse21I CCTNAGG 1 cut(s) 2863
Bse8I GATNNNNATC 1 cut(s) 123
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 5 cut(s) 1344, 1757, 2715, 3071, 3243
BseCI ATCGAT 2 cut(s) 186, 2781
BseJI GATNNNNATC 1 cut(s) 123
BseMII CTCAG 5 cut(s) 170, 374, 1155, 2261, 2877
BseNI ACTGG 6 cut(s) 874, 1465, 1893, 2146, 2161, 3113
BsePI GCGCGC 1 cut(s) 1553
BseRI GAGGAG 2 cut(s) 785, 788
BseSI GKGCMC 2 cut(s) 1092, 2618
BseX3I CGGCCG 1 cut(s) 1529
BseXI GCAGC 6 cut(s) 436, 819, 1507, 1798, 2105, 2563
BseYI CCCAGC 1 cut(s) 303
Bsh1236I CGCG 4 cut(s) 1122, 1555, 1774, 1790
Bsh1285I CGRYCG 1 cut(s) 1532
BshNI GGYRCC 6 cut(s) 587, 766, 787, 1089, 1505, 2654
BshTI ACCGGT 2 cut(s) 763, 2934
BshVI ATCGAT 2 cut(s) 186, 2781
BsiEI CGRYCG 1 cut(s) 1532
BsiHKAI GWGCWC 2 cut(s) 389, 2997
BsiHKCI CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
BsiSI CCGG 9 cut(s) 592, 602, 625, 764, 773, 1497, 1862, 2628, 2935
BslFI GGGAC 7 cut(s) 728, 1313, 1364, 1495, 2420, 2588, 2870
BsmBI CGTCTC 2 cut(s) 1497, 2245
BsmFI GGGAC 7 cut(s) 728, 1313, 1364, 1495, 2420, 2588, 2870
BsmI GAATGC 1 cut(s) 3032
Bso31I GGTCTC 4 cut(s) 67, 98, 1636, 1997
BsoBI CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
Bsp1286I GDGCHC 7 cut(s) 389, 1092, 1503, 1855, 2618, 2634, 2997
Bsp13I TCCGGA 1 cut(s) 601
Bsp19I CCATGG 3 cut(s) 37, 2662, 2850
BspCNI CTCAG 5 cut(s) 169, 375, 1156, 2260, 2876
BspDI ATCGAT 2 cut(s) 186, 2781
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 4 cut(s) 1122, 1555, 1774, 1790
BspHI TCATGA 4 cut(s) 462, 1186, 1221, 2980
BspQI GCTCTTC 1 cut(s) 825
BspT107I GGYRCC 6 cut(s) 587, 766, 787, 1089, 1505, 2654
BspTNI GGTCTC 4 cut(s) 67, 98, 1636, 1997
BsrBI CCGCTC 3 cut(s) 793, 1025, 1526
BsrFI RCCGGY 3 cut(s) 763, 772, 2934
BsrI ACTGG 6 cut(s) 874, 1465, 1893, 2146, 2161, 3113
BssAI RCCGGY 3 cut(s) 763, 772, 2934
BssHII GCGCGC 1 cut(s) 1553
BssNI GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
BssSI CACGAG 3 cut(s) 2760, 2801, 2831
BssT1I CCWWGG 6 cut(s) 37, 779, 860, 1696, 2662, 2850
Bst2BI CACGAG 3 cut(s) 2760, 2801, 2831
Bst2UI CCWGG 5 cut(s) 1344, 1757, 2715, 3071, 3243
Bst4CI ACNGT 5 cut(s) 207, 698, 1647, 1991, 2822
Bst6I CTCTTC 2 cut(s) 825, 2372
BstACI GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
BstAPI GCANNNNNTGC 3 cut(s) 574, 1617, 2339
BstBAI YACGTR 2 cut(s) 680, 1805
BstC8I GCNNGC 9 cut(s) 301, 774, 812, 816, 1027, 1555, 1618, 2091, 3211
BstDEI CTNAG 9 cut(s) 45, 90, 156, 383, 453, 929, 1164, 2247, 2863
BstDSI CCRYGG 6 cut(s) 37, 1468, 2662, 2682, 2850, 2973
BstEII GGTNACC 1 cut(s) 1463
BstFNI CGCG 4 cut(s) 1122, 1555, 1774, 1790
BstH2I RGCGCY 2 cut(s) 791, 2658
BstHHI GCGC 9 cut(s) 201, 790, 1124, 1555, 1557, 1792, 2049, 2657, 3223
BstMCI CGRYCG 1 cut(s) 1532
BstNI CCWGG 5 cut(s) 1344, 1757, 2715, 3071, 3243
BstNSI RCATGY 4 cut(s) 814, 1939, 2093, 2731
BstPAI GACNNNNGTC 3 cut(s) 1784, 2730, 2762
BstPI GGTNACC 1 cut(s) 1463
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 2 cut(s) 1092, 2618
BstUI CGCG 4 cut(s) 1122, 1555, 1774, 1790
BstV1I GCAGC 6 cut(s) 436, 819, 1507, 1798, 2105, 2563
BstV2I GAAGAC 1 cut(s) 3097
BstX2I RGATCY 6 cut(s) 94, 232, 456, 992, 1291, 2895
BstXI CCANNNNNNTGG 1 cut(s) 1516
BstYI RGATCY 6 cut(s) 94, 232, 456, 992, 1291, 2895
BstZI CGGCCG 1 cut(s) 1529
Bsu15I ATCGAT 2 cut(s) 186, 2781
Bsu36I CCTNAGG 1 cut(s) 2863
BsuI GTATCC 1 cut(s) 1703
BsuTUI ATCGAT 2 cut(s) 186, 2781
BtgI CCRYGG 6 cut(s) 37, 1468, 2662, 2682, 2850, 2973
BtgZI GCGATG 1 cut(s) 70
BtrI CACGTC 1 cut(s) 1060
BtsI GCAGTG 3 cut(s) 194, 2118, 2225
BtsIMutI CAGTG 6 cut(s) 194, 867, 2118, 2143, 2154, 2225
Cac8I GCNNGC 9 cut(s) 301, 774, 812, 816, 1027, 1555, 1618, 2091, 3211
CciI TCATGA 4 cut(s) 462, 1186, 1221, 2980
CfoI GCGC 9 cut(s) 201, 790, 1124, 1555, 1557, 1792, 2049, 2657, 3223
Cfr10I RCCGGY 3 cut(s) 763, 772, 2934
Cfr9I CCCGGG 2 cut(s) 1496, 2627
ClaI ATCGAT 2 cut(s) 186, 2781
CpoI CGGWCCG 1 cut(s) 2748
CseI GACGC 8 cut(s) 41, 532, 566, 742, 1076, 1206, 2055, 2720
CsiI ACCWGGT 1 cut(s) 2713
Csp6I GTAC 7 cut(s) 42, 49, 740, 1967, 2143, 2637, 3194
CspAI ACCGGT 2 cut(s) 763, 2934
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CspI CGGWCCG 1 cut(s) 2748
CviQI GTAC 7 cut(s) 42, 49, 740, 1967, 2143, 2637, 3194
DdeI CTNAG 9 cut(s) 45, 90, 156, 383, 453, 929, 1164, 2247, 2863
DinI GGCGCC 2 cut(s) 789, 2656
DrdI GACNNNNNNGTC 1 cut(s) 1489
DseDI GACNNNNNNGTC 1 cut(s) 1489
EaeI YGGCCR 3 cut(s) 735, 1529, 2619
EagI CGGCCG 1 cut(s) 1529
Eam1104I CTCTTC 2 cut(s) 825, 2372
EarI CTCTTC 2 cut(s) 825, 2372
EciI GGCGGA 1 cut(s) 3117
Ecl136II GAGCTC 1 cut(s) 387
EclXI CGGCCG 1 cut(s) 1529
Eco130I CCWWGG 6 cut(s) 37, 779, 860, 1696, 2662, 2850
Eco24I GRGCYC 4 cut(s) 389, 1503, 1855, 2634
Eco31I GGTCTC 4 cut(s) 67, 98, 1636, 1997
Eco32I GATATC 2 cut(s) 1429, 2300
Eco47I GGWCC 7 cut(s) 857, 864, 1712, 1733, 1753, 2243, 2748
Eco52I CGGCCG 1 cut(s) 1529
Eco53kI GAGCTC 1 cut(s) 387
Eco57I CTGAAG 3 cut(s) 429, 2003, 2489
Eco72I CACGTG 1 cut(s) 680
Eco81I CCTNAGG 1 cut(s) 2863
Eco88I CYCGRG 6 cut(s) 711, 1010, 1496, 2031, 2303, 2627
Eco91I GGTNACC 1 cut(s) 1463
EcoICRI GAGCTC 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 2243
EcoO65I GGTNACC 1 cut(s) 1463
EcoRI GAATTC 1 cut(s) 1365
EcoRII CCWGG 5 cut(s) 1342, 1755, 2713, 3069, 3241
EcoRV GATATC 2 cut(s) 1429, 2300
EcoT14I CCWWGG 6 cut(s) 37, 779, 860, 1696, 2662, 2850
EcoT38I GRGCYC 4 cut(s) 389, 1503, 1855, 2634
EgeI GGCGCC 2 cut(s) 789, 2656
EheI GGCGCC 2 cut(s) 789, 2656
ErhI CCWWGG 6 cut(s) 37, 779, 860, 1696, 2662, 2850
Esp3I CGTCTC 2 cut(s) 1497, 2245
FaqI GGGAC 7 cut(s) 728, 1313, 1364, 1495, 2420, 2588, 2870
FauI CCCGC 1 cut(s) 3218
FbaI TGATCA 3 cut(s) 555, 562, 2775
FblI GTMKAC 1 cut(s) 2649
FriOI GRGCYC 4 cut(s) 389, 1503, 1855, 2634
FspAI RTGCGCAY 1 cut(s) 2048
FspBI CTAG 6 cut(s) 138, 705, 1233, 1829, 2592, 2607
FspI TGCGCA 2 cut(s) 200, 2048
GlaI GCGC 9 cut(s) 200, 789, 1123, 1554, 1556, 1791, 2048, 2656, 3222
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 1 cut(s) 1920
HaeII RGCGCY 2 cut(s) 791, 2658
HapII CCGG 9 cut(s) 592, 602, 625, 764, 773, 1497, 1862, 2628, 2935
HgaI GACGC 8 cut(s) 41, 532, 566, 742, 1076, 1206, 2055, 2720
HhaI GCGC 9 cut(s) 201, 790, 1124, 1555, 1557, 1792, 2049, 2657, 3223
Hin1I GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
Hin6I GCGC 9 cut(s) 199, 788, 1122, 1553, 1555, 1790, 2047, 2655, 3221
HinP1I GCGC 9 cut(s) 199, 788, 1122, 1553, 1555, 1790, 2047, 2655, 3221
HincII GTYRAC 4 cut(s) 1581, 2650, 2725, 2877
HindII GTYRAC 4 cut(s) 1581, 2650, 2725, 2877
HindIII AAGCTT 2 cut(s) 1316, 1614
HpaII CCGG 9 cut(s) 592, 602, 625, 764, 773, 1497, 1862, 2628, 2935
HphI GGTGA 5 cut(s) 1457, 1460, 1712, 1847, 2330
Hpy99I CGWCG 4 cut(s) 35, 758, 2651, 2654
HpyAV CCTTC 9 cut(s) 91, 182, 331, 448, 453, 1341, 1402, 2066, 2805
HpyCH4III ACNGT 5 cut(s) 207, 698, 1647, 1991, 2822
HpyCH4IV ACGT 9 cut(s) 24, 679, 1059, 1204, 1490, 1804, 2268, 2398, 2479
HpyF3I CTNAG 9 cut(s) 45, 90, 156, 383, 453, 929, 1164, 2247, 2863
HpySE526I ACGT 9 cut(s) 24, 679, 1059, 1204, 1490, 1804, 2268, 2398, 2479
Hsp92I GRCGYC 6 cut(s) 24, 33, 753, 788, 1490, 2655
HspAI GCGC 9 cut(s) 199, 788, 1122, 1553, 1555, 1790, 2047, 2655, 3221
KasI GGCGCC 2 cut(s) 787, 2654
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 3 cut(s) 555, 562, 2775
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 4 cut(s) 288, 643, 798, 2692
Lsp1109I GCAGC 6 cut(s) 436, 819, 1507, 1798, 2105, 2563
LweI GCATC 6 cut(s) 232, 400, 1753, 1969, 2188, 2514
MabI ACCWGGT 1 cut(s) 2713
MaeI CTAG 6 cut(s) 138, 705, 1233, 1829, 2592, 2607
MaeII ACGT 9 cut(s) 24, 679, 1059, 1204, 1490, 1804, 2268, 2398, 2479
MaeIII GTNAC 7 cut(s) 976, 1060, 1448, 1463, 2152, 2348, 2554
MbiI CCGCTC 3 cut(s) 793, 1025, 1526
MfeI CAATTG 3 cut(s) 616, 1545, 2061
MflI RGATCY 6 cut(s) 94, 232, 456, 992, 1291, 2895
MhlI GDGCHC 7 cut(s) 389, 1092, 1503, 1855, 2618, 2634, 2997
MluI ACGCGT 1 cut(s) 1772
Mly113I GGCGCC 2 cut(s) 788, 2655
MlyI GAGTC 9 cut(s) 405, 1234, 1295, 1301, 1576, 1774, 2525, 2772, 3077
MmeI TCCRAC 3 cut(s) 1757, 2309, 3127
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 772
MseI TTAA 6 cut(s) 518, 1110, 1842, 1875, 2010, 2312
MslI CAYNNNNRTG 6 cut(s) 663, 696, 872, 1446, 2355, 2952
MspA1I CMGCKG 2 cut(s) 449, 2576
MspI CCGG 9 cut(s) 592, 602, 625, 764, 773, 1497, 1862, 2628, 2935
MunI CAATTG 3 cut(s) 616, 1545, 2061
Mva1269I GAATGC 1 cut(s) 3032
MvaI CCWGG 5 cut(s) 1344, 1757, 2715, 3071, 3243
MvnI CGCG 4 cut(s) 1122, 1555, 1774, 1790
NaeI GCCGGC 1 cut(s) 774
NarI GGCGCC 2 cut(s) 788, 2655
NciI CCSGG 6 cut(s) 592, 1497, 1498, 1862, 2628, 2629
NcoI CCATGG 3 cut(s) 37, 2662, 2850
NgoMIV GCCGGC 1 cut(s) 772
NmeAIII GCCGAG 3 cut(s) 1482, 1507, 3156
NmuCI GTSAC 7 cut(s) 976, 1060, 1448, 1463, 2152, 2348, 2554
NsbI TGCGCA 2 cut(s) 200, 2048
NspI RCATGY 4 cut(s) 814, 1939, 2093, 2731
OliI CACNNNNGTG 1 cut(s) 696
PaeI GCATGC 2 cut(s) 814, 2093
PaeR7I CTCGAG 2 cut(s) 2031, 2303
PagI TCATGA 4 cut(s) 462, 1186, 1221, 2980
PalAI GGCGCGCC 1 cut(s) 1553
PasI CCCWGGG 1 cut(s) 3242
PauI GCGCGC 1 cut(s) 1553
PciSI GCTCTTC 1 cut(s) 825
PcsI WCGNNNNNNNCGW 3 cut(s) 1962, 1995, 3003
PctI GAATGC 1 cut(s) 3032
PdiI GCCGGC 1 cut(s) 774
PfeI GAWTC 9 cut(s) 605, 685, 1255, 1268, 1438, 2279, 2456, 2791, 2846
PflFI GACNNNGTC 1 cut(s) 1335
PflMI CCANNNNNTGG 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 1342
PinAI ACCGGT 2 cut(s) 763, 2934
PleI GAGTC 9 cut(s) 404, 1233, 1295, 1301, 1576, 1774, 2525, 2771, 3077
PluTI GGCGCC 2 cut(s) 791, 2658
PmaCI CACGTG 1 cut(s) 680
PmlI CACGTG 1 cut(s) 680
PpsI GAGTC 9 cut(s) 404, 1233, 1295, 1301, 1576, 1774, 2525, 2771, 3077
Ppu21I YACGTR 2 cut(s) 680, 1805
PpuMI RGGWCCY 1 cut(s) 2243
PshAI GACNNNNGTC 3 cut(s) 1784, 2730, 2762
Psp124BI GAGCTC 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 2243
Psp6I CCWGG 5 cut(s) 1342, 1755, 2713, 3069, 3241
PspCI CACGTG 1 cut(s) 680
PspEI GGTNACC 1 cut(s) 1463
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 5 cut(s) 1342, 1755, 2713, 3069, 3241
PspPPI RGGWCCY 1 cut(s) 2243
PsrI GAACNNNNNNTAC 2 cut(s) 1959, 1991
PsuI RGATCY 6 cut(s) 94, 232, 456, 992, 1291, 2895
PsyI GACNNNGTC 1 cut(s) 1335
PteI GCGCGC 1 cut(s) 1553
PvuII CAGCTG 2 cut(s) 449, 2576
RsaI GTAC 7 cut(s) 43, 50, 741, 1968, 2144, 2638, 3195
RsaNI GTAC 7 cut(s) 42, 49, 740, 1967, 2143, 2637, 3194
RseI CAYNNNNRTG 6 cut(s) 663, 696, 872, 1446, 2355, 2952
Rsr2I CGGWCCG 1 cut(s) 2748
RsrII CGGWCCG 1 cut(s) 2748
SacI GAGCTC 1 cut(s) 389
SalI GTCGAC 1 cut(s) 2648
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 6 cut(s) 518, 1110, 1842, 1875, 2010, 2312
SchI GAGTC 9 cut(s) 405, 1234, 1295, 1301, 1576, 1774, 2525, 2772, 3077
SduI GDGCHC 7 cut(s) 389, 1092, 1503, 1855, 2618, 2634, 2997
SexAI ACCWGGT 1 cut(s) 2713
SfaNI GCATC 6 cut(s) 232, 400, 1753, 1969, 2188, 2514
SfcI CTRYAG 1 cut(s) 582
SfoI GGCGCC 2 cut(s) 789, 2656
Sfr274I CTCGAG 2 cut(s) 2031, 2303
SgrAI CRCCGGYG 1 cut(s) 763
SgrDI CGTCGACG 1 cut(s) 2648
SgsI GGCGCGCC 1 cut(s) 1553
SinI GGWCC 7 cut(s) 857, 864, 1712, 1733, 1753, 2243, 2748
SlaI CTCGAG 2 cut(s) 2031, 2303
SmaI CCCGGG 2 cut(s) 1498, 2629
SmiMI CAYNNNNRTG 6 cut(s) 663, 696, 872, 1446, 2355, 2952
SmlI CTYRAG 4 cut(s) 479, 950, 2031, 2303
SmoI CTYRAG 4 cut(s) 479, 950, 2031, 2303
SphI GCATGC 2 cut(s) 814, 2093
SspDI GGCGCC 2 cut(s) 787, 2654
SspI AATATT 1 cut(s) 2085
SspMI CTAG 6 cut(s) 138, 705, 1233, 1829, 2592, 2607
SstI GAGCTC 1 cut(s) 389
StyI CCWWGG 6 cut(s) 37, 779, 860, 1696, 2662, 2850
TaaI ACNGT 5 cut(s) 207, 698, 1647, 1991, 2822
TaiI ACGT 9 cut(s) 27, 682, 1062, 1207, 1493, 1807, 2271, 2401, 2482
TaqII GACCGA 2 cut(s) 116, 2765
TfiI GAWTC 9 cut(s) 605, 685, 1255, 1268, 1438, 2279, 2456, 2791, 2846
Tru1I TTAA 6 cut(s) 518, 1110, 1842, 1875, 2010, 2312
Tru9I TTAA 6 cut(s) 518, 1110, 1842, 1875, 2010, 2312
TscAI CASTG 6 cut(s) 201, 874, 2125, 2143, 2161, 2232
TseFI GTSAC 7 cut(s) 976, 1060, 1448, 1463, 2152, 2348, 2554
TseI GCWGC 6 cut(s) 449, 807, 1520, 1811, 2093, 2576
Tsp45I GTSAC 7 cut(s) 976, 1060, 1448, 1463, 2152, 2348, 2554
TspGWI ACGGA 4 cut(s) 1079, 1209, 2761, 2962
TspMI CCCGGG 2 cut(s) 1496, 2627
TspRI CASTG 6 cut(s) 201, 874, 2125, 2143, 2161, 2232
Tth111I GACNNNGTC 1 cut(s) 1335
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 7 cut(s) 857, 864, 1712, 1733, 1753, 2243, 2748
XapI RAATTY 4 cut(s) 17, 597, 1365, 1904
XceI RCATGY 4 cut(s) 814, 1939, 2093, 2731
XcmI CCANNNNNNNNNTGG 4 cut(s) 234, 1727, 2067, 2153
XhoI CTCGAG 2 cut(s) 2031, 2303
XmaI CCCGGG 2 cut(s) 1496, 2627
XmiI GTMKAC 1 cut(s) 2649
XspI CTAG 6 cut(s) 138, 705, 1233, 1829, 2592, 2607
ZraI GACGTC 2 cut(s) 25, 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.