aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 109, 134 |
| AfaI | GTAC | 1 cut(s) | 52 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 92 |
| AgsI | TTSAA | 2 cut(s) | 109, 125 |
| AluBI | AGCT | 3 cut(s) | 128, 169, 179 |
| AluI | AGCT | 3 cut(s) | 128, 169, 179 |
| Alw21I | GWGCWC | 1 cut(s) | 171 |
| ApoI | RAATTY | 2 cut(s) | 109, 134 |
| AseI | ATTAAT | 1 cut(s) | 8 |
| BanII | GRGCYC | 1 cut(s) | 171 |
| BauI | CACGAG | 1 cut(s) | 35 |
| Bbv12I | GWGCWC | 1 cut(s) | 171 |
| BccI | CCATC | 1 cut(s) | 71 |
| BfaI | CTAG | 2 cut(s) | 176, 214 |
| BsaJI | CCNNGG | 1 cut(s) | 150 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 92 |
| BseDI | CCNNGG | 1 cut(s) | 150 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 92 |
| BseRI | GAGGAG | 1 cut(s) | 75 |
| BsiHKAI | GWGCWC | 1 cut(s) | 171 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 92 |
| Bsp1286I | GDGCHC | 1 cut(s) | 171 |
| BssECI | CCNNGG | 1 cut(s) | 150 |
| BssSI | CACGAG | 1 cut(s) | 35 |
| BssT1I | CCWWGG | 1 cut(s) | 150 |
| Bst2BI | CACGAG | 1 cut(s) | 35 |
| Csp6I | GTAC | 1 cut(s) | 51 |
| CviJI | RGCY | 4 cut(s) | 29, 128, 169, 179 |
| CviKI_1 | RGCY | 4 cut(s) | 29, 128, 169, 179 |
| CviQI | GTAC | 1 cut(s) | 51 |
| DraI | TTTAAA | 1 cut(s) | 115 |
| Ecl136II | GAGCTC | 1 cut(s) | 169 |
| Eco130I | CCWWGG | 1 cut(s) | 150 |
| Eco24I | GRGCYC | 1 cut(s) | 171 |
| Eco53kI | GAGCTC | 1 cut(s) | 169 |
| EcoICRI | GAGCTC | 1 cut(s) | 169 |
| EcoT14I | CCWWGG | 1 cut(s) | 150 |
| EcoT38I | GRGCYC | 1 cut(s) | 171 |
| ErhI | CCWWGG | 1 cut(s) | 150 |
| FaiI | YATR | 4 cut(s) | 21, 79, 144, 188 |
| FriOI | GRGCYC | 1 cut(s) | 171 |
| FspBI | CTAG | 2 cut(s) | 176, 214 |
| Hpy166II | GTNNAC | 1 cut(s) | 60 |
| Hpy8I | GTNNAC | 1 cut(s) | 60 |
| MaeI | CTAG | 2 cut(s) | 176, 214 |
| MboII | GAAGA | 1 cut(s) | 167 |
| MhlI | GDGCHC | 1 cut(s) | 171 |
| MluCI | AATT | 2 cut(s) | 109, 134 |
| MnlI | CCTC | 2 cut(s) | 93, 96 |
| MseI | TTAA | 2 cut(s) | 8, 114 |
| PshBI | ATTAAT | 1 cut(s) | 8 |
| Psp124BI | GAGCTC | 1 cut(s) | 171 |
| RsaI | GTAC | 1 cut(s) | 52 |
| RsaNI | GTAC | 1 cut(s) | 51 |
| SacI | GAGCTC | 1 cut(s) | 171 |
| SaqAI | TTAA | 2 cut(s) | 8, 114 |
| SduI | GDGCHC | 1 cut(s) | 171 |
| SetI | ASST | 4 cut(s) | 130, 171, 181, 215 |
| SgeI | CNNG | 5 cut(s) | 47, 49, 137, 163, 188 |
| Sse9I | AATT | 2 cut(s) | 109, 134 |
| SspMI | CTAG | 2 cut(s) | 176, 214 |
| SstI | GAGCTC | 1 cut(s) | 171 |
| StyI | CCWWGG | 1 cut(s) | 150 |
| TaqI | TCGA | 1 cut(s) | 97 |
| TasI | AATT | 2 cut(s) | 109, 134 |
| Tru1I | TTAA | 2 cut(s) | 8, 114 |
| Tru9I | TTAA | 2 cut(s) | 8, 114 |
| TspDTI | ATGAA | 2 cut(s) | 159, 175 |
| VspI | ATTAAT | 1 cut(s) | 8 |
| XapI | RAATTY | 2 cut(s) | 109, 134 |
| XspI | CTAG | 2 cut(s) | 176, 214 |