FvH4_5g36310

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000952
Physical Location & Seq
Forward (+)
26628557 .. 26630937
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36310.t1

Sequence Viewer

Length: 1950 bp
ATGGCAAGTACTCCAGTTGGATCTAATGCACTTATTCAGGAAGTTCTTGACCGCAGTAACTATAAAAAGTGGAGATTACGGTTAAAAACTTATTTCTTGTCTGAAGATCTATGGGATATTGTAGAAGCAAACACGGAACCTCCAAAACTAGAAGATGATCAAGCCGGTTATAAGGCTTGGACCAAAAAGAATGCCAAGGCTTTATATGCAATCCAAAGCTCATGCGGGCCGGAGATGTTTACTTTTATAAGCGAAACTGAATCGGCCGAAGCTGCCTGGGAGGCTTTGGAACAAGAGTGCAAGGTTTTGCTAGGATATGATGAGAATAATGCCGACCACTTGGTGCGCTATAAATCGTTCATCGGATATGTACGGAGAGGCGATTGGGATAGGGCAAAGAAGTGCCTAGGAGAAGTTGATGTGCGTAGTGTGATAGTAAGAGCAATAGCTAGGTGGGACGGGATGACAGCTCTTCATGTAGCAGCCTGGGAAGGGCACGCGCATATTGTTAAAGAGTTGGTGCATTTGATGACGCAAGAAGATTGCAAAACAAAAACCGGTTCTGGTCGTACAGCTCTTCACCTTGCAGCACAGCAGGGGCATGTTCATGTTGTGAAAGAGTTGCCGGTGCAGTTGATGGGAGAAGTACAAGACAAAGGTGGTGACACAGCTCTTCATGATGCAGTCCACTCAGGGAAGGTCGATGTTGTGAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACTTGCAAATAAAAAACCATGCTGGTTACACAGCTCTTCACCTAGCAGTGAATAAGGGGAATATGCCTATGGTCAAAGAGTTCGTGACAAAAGAAAAAGGTGAGAACAATATTGTCATGGACGATTTTGAGCGTTATATACCGTTTACCACCTATGTGAAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATGATCCATGTGGTGCAGTAACAGCAGTAGATCCAAGAGACAACTACCGAGACACTGCTCTTCACGTGGCCGCCCGGGAAGGGCATCTGGATGTTATCAGAGGATTGATCTTGTTGCTGAAAATGAGACGAGAAGATTTGGAGTTGAAAACAGCACAAGATTTCACAGCTTATGGTTCTGGTATAACTTTAGGGGTCACTGAAGAATTTCTCATGGAAAGGGCCAAATACATGGTTGAACAGTACGAGAAAACACATGGTATTTTTTTGGAGATGCAAAATTCTGAAGGTTGTACAGCTCTTTACCTAGCAGTACGGGTGGGAAATGTTGATATTGTGAAAGAGTTGGCGCATATGATGACACAAGAAGGTTTGGAACTGAAAAGCTTCAGTGGTTCCAGTGCTCTTCACCAAGCAATCGGGTATGAGCGTATAGATATTGTGCAAGTGTTGGTGCCCTTGATGAGAAAAGAAGGTTTAGAAATTAAAGACGACCATGGAGACACACCTCTTAACTATGCCGTCTTAAAAGGGAATGTGGATATTGTGAAGGCATTGGTGCAATCCATGAGACAAGAAGGTTTGGAAATAAGAAACAGCAAGGCTGAGATTGCTCTTCCCCGAGCAATATCGAAGGGACATATGCACATTGTTAGAGAGTTGTTGCCGTTGATGAGACCAGAGGCTTTCGAAGAAAAAGACGGTCGCGGCTGTACAGCTTTATGTGGGGCTATTGCATGTTTGAAGGATGGAGAAGTGTACGAGATAGCTAAGTACATGGCTGAAAGAAATGATAGAGTATTTGGCATCCCCTCAGCTCCCTATGATGAAATTCCAGTTGTCCAAGCTCTTCGTTGGAGGAAATGGAGATTGGCTCGCTCTATCTATTCTCTCACTCCACTTGAACTTATCGACCAACCTAATGGCGGTGCATTTATTTGCTATTGTCTTGATACTAGCACACAGATTGGTAAGACTGTCAATTTTAGTAACCATTTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

650

Amino Acids

73.08

Weight (kDa)

5.73

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 1.9e-06 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 1.3e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 148 - 208 5e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 154 - 196 1.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 158 - 241 1.5e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 252 - 352 1.7e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 446 - 504 4.6e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 446 - 489 9.8e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 248
AccB1I GGYRCC 1 cut(s) 1399
AccII CGCG 2 cut(s) 500, 1653
AciI CCGC 5 cut(s) 52, 225, 1017, 1653, 1872
AclWI GGATC 3 cut(s) 28, 944, 971
AcoI YGGCCR 2 cut(s) 264, 1014
AcsI RAATTY 3 cut(s) 1151, 1225, 1776
AcuI CTGAAG 4 cut(s) 123, 1167, 1251, 1318
AcvI CACGTG 1 cut(s) 1012
AdeI CACNNNGTG 1 cut(s) 343
AfiI CCNNNNNNNGG 3 cut(s) 492, 1026, 1871
AgeI ACCGGT 1 cut(s) 557
AgsI TTSAA 4 cut(s) 1093, 1184, 1690, 1850
AjnI CCWGG 2 cut(s) 275, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 1799, 1831
AleI CACNNNNGTG 1 cut(s) 896
Alw21I GWGCWC 1 cut(s) 1351
Alw26I GTCTC 7 cut(s) 727, 978, 990, 1066, 1440, 1510, 1615
AlwI GGATC 3 cut(s) 28, 944, 971
Ama87I CYCGRG 2 cut(s) 1020, 1566
AoxI GGCC 4 cut(s) 227, 264, 1014, 1167
ApeKI GCWGC 3 cut(s) 272, 482, 587
ApoI RAATTY 3 cut(s) 1151, 1225, 1776
AsiGI ACCGGT 1 cut(s) 557
Asp700I GAANNNNTTC 2 cut(s) 1152, 1331
AspA2I CCTAGG 1 cut(s) 406
AspLEI GCGC 3 cut(s) 348, 502, 1297
AspS9I GGNCC 3 cut(s) 180, 227, 1167
AsuC2I CCSGG 2 cut(s) 1021, 1022
AsuHPI GGTGA 6 cut(s) 572, 674, 773, 854, 920, 1346
AsuII TTCGAA 1 cut(s) 1635
AvaI CYCGRG 2 cut(s) 1020, 1566
AvaII GGWCC 1 cut(s) 180
AvrII CCTAGG 1 cut(s) 406
BaeGI GKGCMC 2 cut(s) 498, 1404
BanI GGYRCC 1 cut(s) 1399
BbrPI CACGTG 1 cut(s) 1012
BbsI GAAGAC 1 cut(s) 747
Bbv12I GWGCWC 1 cut(s) 1351
BbvCI CCTCAGC 1 cut(s) 1759
BbvI GCAGC 3 cut(s) 259, 494, 599
BccI CCATC 2 cut(s) 631, 1688
BceAI ACGGC 2 cut(s) 1451, 1597
BciT130I CCWGG 2 cut(s) 277, 487
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 2 cut(s) 1021, 1022
BcoDI GTCTC 7 cut(s) 727, 978, 990, 1066, 1440, 1510, 1615
BfaI CTAG 8 cut(s) 149, 311, 407, 450, 785, 944, 1253, 1902
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 273, 483, 588, 1017, 1654
BlnI CCTAGG 1 cut(s) 406
BlsI GCNGC 5 cut(s) 274, 484, 589, 1018, 1655
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 4 cut(s) 277, 487, 1021, 1022
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 2 cut(s) 1020, 1566
BmgT120I GGNCC 3 cut(s) 180, 227, 1167
BmiI GGNNCC 3 cut(s) 138, 1342, 1401
BmrFI CCNGG 4 cut(s) 277, 487, 1021, 1022
BmsI GCATC 4 cut(s) 670, 1039, 1209, 1761
BpiI GAAGAC 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 1804, 1836
Bpu10I CCTNAGC 1 cut(s) 1759
Bpu14I TTCGAA 1 cut(s) 1635
BpuMI CCSGG 2 cut(s) 1021, 1022
BsaAI YACGTR 1 cut(s) 1012
BsaI GGTCTC 1 cut(s) 1615
BsaJI CCNNGG 6 cut(s) 195, 276, 406, 486, 1020, 1441
BsaWI WCCGGW 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 1026, 1871
Bse118I RCCGGY 3 cut(s) 164, 557, 625
Bse1I ACTGG 3 cut(s) 14, 1344, 1781
BseBI CCWGG 2 cut(s) 277, 487
BseDI CCNNGG 6 cut(s) 195, 276, 406, 486, 1020, 1441
BseGI GGATG 4 cut(s) 468, 1042, 1699, 1752
BseLI CCNNNNNNNGG 3 cut(s) 492, 1026, 1871
BseMII CTCAG 3 cut(s) 705, 1542, 1773
BseNI ACTGG 3 cut(s) 14, 1344, 1781
BseSI GKGCMC 2 cut(s) 498, 1404
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 3 cut(s) 259, 494, 599
BsgI GTGCAG 2 cut(s) 650, 981
Bsh1236I CGCG 2 cut(s) 500, 1653
Bsh1285I CGRYCG 2 cut(s) 267, 1651
BshFI GGCC 4 cut(s) 229, 266, 1016, 1169
BshNI GGYRCC 1 cut(s) 1399
BshTI ACCGGT 1 cut(s) 557
BsiEI CGRYCG 2 cut(s) 267, 1651
BsiHKAI GWGCWC 1 cut(s) 1351
BsiHKCI CYCGRG 2 cut(s) 1020, 1566
BsiSI CCGG 5 cut(s) 165, 230, 558, 626, 1021
BslFI GGGAC 2 cut(s) 470, 1596
BslI CCNNNNNNNGG 3 cut(s) 492, 1026, 1871
BsmAI GTCTC 7 cut(s) 727, 978, 990, 1066, 1440, 1510, 1615
BsmBI CGTCTC 1 cut(s) 1066
BsmFI GGGAC 2 cut(s) 470, 1596
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 4 cut(s) 229, 266, 1016, 1169
Bso31I GGTCTC 1 cut(s) 1615
BsoBI CYCGRG 2 cut(s) 1020, 1566
Bsp119I TTCGAA 1 cut(s) 1635
Bsp1286I GDGCHC 3 cut(s) 498, 1351, 1404
Bsp1407I TGTACA 2 cut(s) 1238, 1658
Bsp143I GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
Bsp19I CCATGG 1 cut(s) 1441
BspACI CCGC 5 cut(s) 52, 225, 1017, 1653, 1872
BspANI GGCC 4 cut(s) 229, 266, 1016, 1169
BspCNI CTCAG 3 cut(s) 704, 1543, 1772
BspFNI CGCG 2 cut(s) 500, 1653
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 138, 1342, 1401
BspPI GGATC 3 cut(s) 28, 944, 971
BspQI GCTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
BspT104I TTCGAA 1 cut(s) 1635
BspT107I GGYRCC 1 cut(s) 1399
BspTNI GGTCTC 1 cut(s) 1615
BsrFI RCCGGY 3 cut(s) 164, 557, 625
BsrGI TGTACA 2 cut(s) 1238, 1658
BsrI ACTGG 3 cut(s) 14, 1344, 1781
BssAI RCCGGY 3 cut(s) 164, 557, 625
BssECI CCNNGG 6 cut(s) 195, 276, 406, 486, 1020, 1441
BssMI GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
BssT1I CCWWGG 3 cut(s) 195, 406, 1441
Bst2UI CCWGG 2 cut(s) 277, 487
Bst4CI ACNGT 5 cut(s) 81, 885, 1188, 1649, 1924
Bst6I CTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
BstAUI TGTACA 2 cut(s) 1238, 1658
BstBAI YACGTR 1 cut(s) 1012
BstBI TTCGAA 1 cut(s) 1635
BstC8I GCNNGC 3 cut(s) 227, 498, 1822
BstDEI CTNAG 5 cut(s) 691, 935, 1551, 1716, 1759
BstDSI CCRYGG 1 cut(s) 1441
BstF5I GGATG 4 cut(s) 468, 1042, 1699, 1752
BstFNI CGCG 2 cut(s) 500, 1653
BstHHI GCGC 3 cut(s) 348, 502, 1297
BstKTI GATC 6 cut(s) 23, 109, 160, 952, 979, 1056
BstMAI GTCTC 7 cut(s) 727, 978, 990, 1066, 1440, 1510, 1615
BstMBI GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
BstMCI CGRYCG 2 cut(s) 267, 1651
BstMWI GCNNNNNNNGC 5 cut(s) 206, 272, 281, 968, 1556
BstNI CCWGG 2 cut(s) 277, 487
BstNSI RCATGY 2 cut(s) 605, 1686
BstSCI CCNGG 4 cut(s) 275, 485, 1019, 1020
BstSLI GKGCMC 2 cut(s) 498, 1404
BstUI CGCG 2 cut(s) 500, 1653
BstV1I GCAGC 3 cut(s) 259, 494, 599
BstV2I GAAGAC 1 cut(s) 747
BstX2I RGATCY 3 cut(s) 20, 106, 976
BstXI CCANNNNNNTGG 2 cut(s) 1177, 1868
BstYI RGATCY 3 cut(s) 20, 106, 976
BstZI CGGCCG 1 cut(s) 264
BsuRI GGCC 4 cut(s) 229, 266, 1016, 1169
BtgI CCRYGG 1 cut(s) 1441
BtsCI GGATG 4 cut(s) 468, 1042, 1699, 1752
BtsI GCAGTG 2 cut(s) 795, 999
BtsIMutI CAGTG 5 cut(s) 795, 999, 1143, 1342, 1351
Cac8I GCNNGC 3 cut(s) 227, 498, 1822
CciI TCATGA 1 cut(s) 676
CfoI GCGC 3 cut(s) 348, 502, 1297
Cfr10I RCCGGY 3 cut(s) 164, 557, 625
Cfr13I GGNCC 3 cut(s) 180, 227, 1167
Cfr9I CCCGGG 1 cut(s) 1020
CseI GACGC 1 cut(s) 541
CspAI ACCGGT 1 cut(s) 557
DdeI CTNAG 5 cut(s) 691, 935, 1551, 1716, 1759
DpnI GATC 6 cut(s) 22, 108, 159, 951, 978, 1055
DpnII GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 2 cut(s) 264, 1014
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
EarI CTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 195, 406, 1441
Eco31I GGTCTC 1 cut(s) 1615
Eco47I GGWCC 1 cut(s) 180
Eco52I CGGCCG 1 cut(s) 264
Eco57I CTGAAG 4 cut(s) 123, 1167, 1251, 1318
Eco72I CACGTG 1 cut(s) 1012
Eco88I CYCGRG 2 cut(s) 1020, 1566
EcoRII CCWGG 2 cut(s) 275, 485
EcoT14I CCWWGG 3 cut(s) 195, 406, 1441
ErhI CCWWGG 3 cut(s) 195, 406, 1441
Esp3I CGTCTC 1 cut(s) 1066
FaqI GGGAC 2 cut(s) 470, 1596
FauI CCCGC 1 cut(s) 218
FauNDI CATATG 2 cut(s) 1299, 1587
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 273, 483, 588, 1017, 1654
FokI GGATG 4 cut(s) 475, 1049, 1706, 1739
Fsp4HI GCNGC 5 cut(s) 273, 483, 588, 1017, 1654
FspBI CTAG 8 cut(s) 149, 311, 407, 450, 785, 944, 1253, 1902
GlaI GCGC 3 cut(s) 347, 501, 1296
GluI GCNGC 5 cut(s) 273, 483, 588, 1017, 1654
HaeIII GGCC 4 cut(s) 229, 266, 1016, 1169
HapII CCGG 5 cut(s) 165, 230, 558, 626, 1021
HgaI GACGC 1 cut(s) 541
HhaI GCGC 3 cut(s) 348, 502, 1297
Hin6I GCGC 3 cut(s) 346, 500, 1295
HinP1I GCGC 3 cut(s) 346, 500, 1295
HindIII AAGCTT 1 cut(s) 1330
HinfI GANTC 1 cut(s) 260
HpaII CCGG 5 cut(s) 165, 230, 558, 626, 1021
HphI GGTGA 6 cut(s) 572, 674, 773, 854, 920, 1346
Hpy166II GTNNAC 4 cut(s) 240, 688, 888, 1705
Hpy188I TCNGA 4 cut(s) 103, 365, 1046, 1231
Hpy188III TCNNGA 6 cut(s) 38, 47, 677, 826, 1034, 1895
Hpy8I GTNNAC 4 cut(s) 240, 688, 888, 1705
HpyCH4III ACNGT 5 cut(s) 81, 885, 1188, 1649, 1924
HpyCH4IV ACGT 1 cut(s) 1011
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 272, 281, 968, 1556
HpyF3I CTNAG 5 cut(s) 691, 935, 1551, 1716, 1759
HpySE526I ACGT 1 cut(s) 1011
HspAI GCGC 3 cut(s) 346, 500, 1295
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
LguI GCTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
LmnI GCTCC 1 cut(s) 1768
Lsp1109I GCAGC 3 cut(s) 259, 494, 599
LweI GCATC 4 cut(s) 670, 1039, 1209, 1761
MaeI CTAG 8 cut(s) 149, 311, 407, 450, 785, 944, 1253, 1902
MaeII ACGT 1 cut(s) 1011
MaeIII GTNAC 7 cut(s) 56, 662, 767, 826, 964, 1141, 1934
MalI GATC 6 cut(s) 22, 108, 159, 951, 978, 1055
MboI GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
MflI RGATCY 3 cut(s) 20, 106, 976
MhlI GDGCHC 3 cut(s) 498, 1351, 1404
MluCI AATT 5 cut(s) 1151, 1225, 1428, 1776, 1927
MmeI TCCRAC 1 cut(s) 1781
MnlI CCTC 8 cut(s) 150, 274, 371, 1040, 1464, 1621, 1768, 1797
MroXI GAANNNNTTC 2 cut(s) 1152, 1331
MseI TTAA 6 cut(s) 83, 510, 1431, 1458, 1472, 1944
MslI CAYNNNNRTG 3 cut(s) 606, 896, 1035
MspI CCGG 5 cut(s) 165, 230, 558, 626, 1021
MspR9I CCNGG 4 cut(s) 277, 487, 1021, 1022
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 2 cut(s) 277, 487
MvnI CGCG 2 cut(s) 500, 1653
MwoI GCNNNNNNNGC 5 cut(s) 206, 272, 281, 968, 1556
NciI CCSGG 2 cut(s) 1021, 1022
NcoI CCATGG 1 cut(s) 1441
NdeI CATATG 2 cut(s) 1299, 1587
NdeII GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
NlaIV GGNNCC 3 cut(s) 138, 1342, 1401
NmuCI GTSAC 3 cut(s) 662, 826, 1141
NspI RCATGY 2 cut(s) 605, 1686
NspV TTCGAA 1 cut(s) 1635
OliI CACNNNNGTG 1 cut(s) 896
PagI TCATGA 1 cut(s) 676
PciSI GCTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
PctI GAATGC 1 cut(s) 196
PdmI GAANNNNTTC 2 cut(s) 1152, 1331
PfeI GAWTC 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 557
PkrI GCNGC 5 cut(s) 274, 484, 589, 1018, 1655
PmaCI CACGTG 1 cut(s) 1012
PmlI CACGTG 1 cut(s) 1012
Ppu21I YACGTR 1 cut(s) 1012
PsiI TTATAA 2 cut(s) 171, 248
Psp6I CCWGG 2 cut(s) 275, 485
PspCI CACGTG 1 cut(s) 1012
PspGI CCWGG 2 cut(s) 275, 485
PspN4I GGNNCC 3 cut(s) 138, 1342, 1401
PspPI GGNCC 3 cut(s) 180, 227, 1167
PsuI RGATCY 3 cut(s) 20, 106, 976
RseI CAYNNNNRTG 3 cut(s) 606, 896, 1035
SapI GCTCTTC 8 cut(s) 477, 582, 678, 783, 1011, 1356, 1566, 1800
SaqAI TTAA 6 cut(s) 83, 510, 1431, 1458, 1472, 1944
SatI GCNGC 5 cut(s) 273, 483, 588, 1017, 1654
Sau3AI GATC 6 cut(s) 20, 106, 157, 949, 976, 1053
Sau96I GGNCC 3 cut(s) 180, 227, 1167
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 4 cut(s) 277, 487, 1021, 1022
SduI GDGCHC 3 cut(s) 498, 1351, 1404
SfaNI GCATC 4 cut(s) 670, 1039, 1209, 1761
SfuI TTCGAA 1 cut(s) 1635
SinI GGWCC 1 cut(s) 180
SmaI CCCGGG 1 cut(s) 1022
SmiMI CAYNNNNRTG 3 cut(s) 606, 896, 1035
Sse9I AATT 5 cut(s) 1151, 1225, 1428, 1776, 1927
SsiI CCGC 5 cut(s) 52, 225, 1017, 1653, 1872
SspI AATATT 1 cut(s) 853
SspMI CTAG 8 cut(s) 149, 311, 407, 450, 785, 944, 1253, 1902
StyD4I CCNGG 4 cut(s) 275, 485, 1019, 1020
StyI CCWWGG 3 cut(s) 195, 406, 1441
TaaI ACNGT 5 cut(s) 81, 885, 1188, 1649, 1924
TaiI ACGT 1 cut(s) 1014
TaqI TCGA 4 cut(s) 702, 1577, 1635, 1857
TasI AATT 5 cut(s) 1151, 1225, 1428, 1776, 1927
TatI WGTACW 5 cut(s) 8, 646, 1238, 1658, 1719
TauI GCSGC 2 cut(s) 1019, 1656
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 6 cut(s) 83, 510, 1431, 1458, 1472, 1944
Tru9I TTAA 6 cut(s) 83, 510, 1431, 1458, 1472, 1944
TscAI CASTG 5 cut(s) 795, 1006, 1150, 1342, 1351
TseFI GTSAC 3 cut(s) 662, 826, 1141
TseI GCWGC 3 cut(s) 272, 482, 587
Tsp45I GTSAC 3 cut(s) 662, 826, 1141
TspDTI ATGAA 5 cut(s) 349, 464, 596, 665, 1788
TspGWI ACGGA 2 cut(s) 149, 388
TspMI CCCGGG 1 cut(s) 1020
TspRI CASTG 5 cut(s) 795, 1006, 1150, 1342, 1351
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 3 cut(s) 1151, 1225, 1776
XceI RCATGY 2 cut(s) 605, 1686
XmaI CCCGGG 1 cut(s) 1020
XmaJI CCTAGG 1 cut(s) 406
XmnI GAANNNNTTC 2 cut(s) 1152, 1331
XspI CTAG 8 cut(s) 149, 311, 407, 450, 785, 944, 1253, 1902
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.