FvH4_5g37090

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000952
Physical Location & Seq
Reverse (-)
27161963 .. 27184483
22521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37090.t4

Sequence Viewer

Length: 3378 bp
ATGGAAAGTATTCAAGTTGGCTCTAATGCACTTATTATGGAAGTTCTTGATCGAAATAACTACAAGCATTGGAGATCGCGGTTGAAAACTTATTTATTGGCTGAAGATCTCTGGGAGGTTGTTGAAGCAATCAGAGAGACTCCAAAACTCAAAGGTGATCAAGCCGATTACAAGGCCTGGACCATAAAGAATGCGAAGGCTTTGTACGCAATCCAAAATTCATGTGGAAGAGAGCTCTTCTCTTTCATAAGCGAAATTGAGACGGCCAGAAGTGCTTGGGAGACGTTGGAACGAGAGTGCGAAGTTATGCAAGGATTTGATGAGAATAATTCCGACCACTTGGAGCGCTATAAATCGTTTGTCAGATATGTGGAGAGTGGTGATTGGGACAAGGCAAAGAAGTGTCTTAGAGAAATTGATGTCCGCAGTGTCTTAGAAGTAAGAGCAATAGACGGACGGAACGGAACAACAGCTCTTCATGTAGCGGCCATGGAAGGGCACGCGCGTATAGTGAAAGATTTGGTGCATTTGATGACGCAAGAAGATTTGAAAACAAAAACCGATTCTGGTCACACAGCTCTTCATCTAGCAGCACAGCAGGGGCATGTTCATGTTGTGAAAGAGTTGCCGGTGCAGTTGATGGGAGAAGTACAAGACAAAGGTGGTCACACAGCCCTTCATTATGCAGTCCACGCAGGGAAGGTCGATGTTGTGAAAGAGTTGTTGCTGTTGATGAGACAAGATGACTTTTTGCAAATTAAAAACAAGGATGGTGATACAGCTCTTCATATAGCAGTTCAGAAAGGGAATGTCGATATCGTGAAAGAGTTGGTGCTGTTGATGAGACAAGAAGACTTGAAAACAAAAAACGATGCTGGTTACACAGCTCTTCATCTAGCCGTCAGTAAGGGGAATGTCCCTATGGTCAAACCGTTCGTGATAAAAGAAAAAGGTGAGGCTAATATTGGCGAAGATGAGTTTGAGCGTTATATACCGTTTACCACCTATGTGACAACTGGTGATCGGGATAAGGCAAAAGGGTGTCTTAAAGAACTAGATGATCCTTGTGGTGCAGTAACAGTAGTAGATCCCAGAGACGACTTGAGAGAAACTGCTCTTCACGTAGCAGCCCGGAGAGGGCATGTGGATGTTATCAAAGAGTTGTTGTTGTTGCTGAAAAGGCGAGAAGAAGATCATCTGGAGTTGAAAACCGCTGAAGATTTCACCATTTTTGGTTCTGGTATAAGTTTAGGGGTTAGTGAAGAGTTCCTTATGGCAAGGGCAAAATACATGGTTGAACAATACGAGAAAACACTTGGCAGTATTTTGGAGATACAGAATTATAATGGTTCCACACCTCTTCACGTAGCAGTAATAGAGGGGCATGCGCATATTGTGAAAGAGTTGGTTCATTTCATGAGAAAAGAAGGTTTAGAAAAACAAAACCATTATGGAGAATCACCTCTTCACGTAGCAGTAATAAAGGAGAATGTGCTTATTGTGAAAGAGTTGGTTCAGTTCATGAGAAAAGAAGGTTTAGAAATACAAAACCGTTATGGAGAATCACCTCTTCACGTAGCAGTAATAAAGCAGAATGTGCGTATTGTGAAAGAGTTGGTACCATGGATGAGACGAGATGGTATGGAAATAAAAGATTCCGGGGGTTCTTATGCACTTCACCGAGCAGTCAGGAATAGACATGTACAAATCGTCAAAGAGCTGACGTTATGTATGATACCAGAAGCTTTGGAAATAAAAGACGGCCAAGGCTATACAGCTTTAGGCCGTGCTCTAAATGTAGGCAGGGATAGAGACGCGATGGAAATGGCGAGATACATGGCTGAAAGGAATCATAAAGTAGTTGGCATTCCGGGGCCTTTTGATGATGATGACATTCCAGTTGTCAGCGCTCTTCGTGGTGGCCTGTGGAAGTCAGCTCGATATCTGTATTCTATTACTCCACCGGAAGTTCTCCACGGATCCAATGGCAGTCAATTTATTTGCTATTGTTTGAACTCTAGCAGAACGCTTGATATGGCATGGGACTTGCTTCAACGTTACCCAATCTTGGCCATGACTGTTTACGAAACGTATAAAACTTCTCCTATGTTGAGGTTGGCTAGCATGCGTTTTGCATTTTTAAGTGCAACACGACTCACACGTTTGCAAAGGTGGATTTATGACTGTATACACATAGGAGATTATCAAGTTAACAATCTTGATATCTCTATAAATGTTGTCGAATCAAAACATGGTCATGACGATAAAAGGAGTTTTATTTCCTCAGTTATGAATTCATTTAGAAGATTTTCCAAAGGTGTCGGTAAATTTTGCGGAATCGATTTTATATATCATATGAAATCGACACATGAATGGATCTTTCTTTTTCTACATTGCATGGGTGAAACGACAAAATATCATCATCTCACTCCAGAGCAACTTGAAATGGTTAAAAAATCAGTCTTCGTTGCTATAGAAAAAGGGCATTTTGAGTTTGTTACTCGACTATGTAAAACAAATCCAAGAATACTCACGGATACTTATGATGAACATGGGAGGACCATATTTCATGTTGCCGTTCAATATCGTCAAGAAGAGGTTTATAGTCTTATGTATGGGCTTGATGAAGAAAAGCAGTCTGAAATTGGAATTACTCTAGTGAACACTGACTATAATCTGCTACATATTGCTGCCGATTTATTCTCGGGTTCATATGGTGATCGTATTCAAGATGCAAGTTTGCGACTACAAAGAGAACTACAGTGGTTTAAGGAGGTGGGAAGTATTGTGCCTATCGAAATGCATGAGTATCGAAGACATTCAGATGGTTTGACAGCACGTGAACTATTTACCAAGACTCACAAGAACTTGATAGAGGATGCAAAAAGTTCAATGATAGAAACAGCACATTCTTGTACAGTTATAGGTGCTCTTATTGTTACAATGATGTTTGCTGCAACTTTCACACTTCCTGGTGGGAATAATGGTAACACAGGTCTTCCCTTTTTCCAAGATAACAAGTTGTTAAAGGTTTTTATAGTTTCAGATACTATATCACTTGTGTCTTCCACAATTTCTGTGATTACGTTCTTGGGAATTCTCACCTCGAGTTACGCAGAAGATGATTTCCTCAAATATCTGCTCACAATGACAAAGATTGGGATATTCACTCTTTTCTTCTCTATTGTCACCATGATGATTGTGTTTTCTTCTGCACTTGCCATTATGCTTGAGGGGGAGGCATGGATTGTTATACCAAGTATTTTGTTCGCTAGTCTTCCGGCCCTCTATTTCTCATGGTTGTTGTTCCCTCTCCTTGGCAAGATGTTCAAGTCTACTTATGGACCTGGAAGATTTGGCAGAAGAAGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

1126

Amino Acids

128.02

Weight (kDa)

6.54

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 81 1.6e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 55 - 116 2.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 77 - 150 1e-08 Ankyrin repeats (3 copies)
PGG PF13962 541 - 650 2e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1344
Acc16I TGCGCA 1 cut(s) 1389
Acc65I GGTACC 1 cut(s) 1618
AccB1I GGYRCC 1 cut(s) 1618
AccI GTMKAC 2 cut(s) 2188, 3335
AccII CGCG 4 cut(s) 79, 503, 505, 1817
AciI CCGC 5 cut(s) 79, 424, 485, 1212, 2334
AclI AACGTT 1 cut(s) 2056
AclWI GGATC 5 cut(s) 1055, 1082, 1974, 1987, 2384
AcoI YGGCCR 4 cut(s) 264, 486, 1762, 2070
AcsI RAATTY 4 cut(s) 217, 2293, 2327, 3096
AcuI CTGAAG 2 cut(s) 123, 1236
AcvI CACGTG 1 cut(s) 2840
AfaI GTAC 5 cut(s) 206, 651, 1620, 1704, 2917
AfeI AGCGCT 2 cut(s) 347, 1909
AfiI CCNNNNNNNGG 4 cut(s) 495, 1137, 2068, 3317
AflIII ACRYGT 2 cut(s) 1699, 2159
AjnI CCWGG 3 cut(s) 176, 2971, 3346
AleI CACNNNNGTG 1 cut(s) 1007
AluBI AGCT 9 cut(s) 235, 473, 578, 782, 887, 1720, 1745, 1778, 1937
AluI AGCT 9 cut(s) 235, 473, 578, 782, 887, 1720, 1745, 1778, 1937
Alw21I GWGCWC 3 cut(s) 237, 1792, 2932
Alw26I GTCTC 8 cut(s) 131, 254, 275, 730, 838, 1089, 1624, 1806
AlwI GGATC 5 cut(s) 1055, 1082, 1974, 1987, 2384
Ama87I CYCGRG 2 cut(s) 2704, 3106
Aor51HI AGCGCT 2 cut(s) 347, 1909
AoxI GGCC 9 cut(s) 174, 264, 486, 1762, 1783, 1874, 1921, 2070, 3282
ApeKI GCWGC 4 cut(s) 590, 1127, 2690, 2954
ApoI RAATTY 4 cut(s) 217, 2293, 2327, 3096
Asp700I GAANNNNTTC 3 cut(s) 9, 2308, 2818
Asp718I GGTACC 1 cut(s) 1618
AspLEI GCGC 4 cut(s) 348, 505, 1390, 1910
AspS9I GGNCC 5 cut(s) 180, 1874, 2559, 3283, 3344
AsuC2I CCSGG 3 cut(s) 1132, 1660, 1872
AsuNHI GCTAGC 1 cut(s) 2120
AvaI CYCGRG 2 cut(s) 2704, 3106
AvaII GGWCC 3 cut(s) 180, 2559, 3344
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 2072
BamHI GGATCC 1 cut(s) 1979
BanI GGYRCC 1 cut(s) 1618
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbrPI CACGTG 1 cut(s) 2840
BbsI GAAGAC 6 cut(s) 858, 2455, 2821, 2990, 3057, 3269
Bbv12I GWGCWC 3 cut(s) 237, 1792, 2932
BbvI GCAGC 4 cut(s) 602, 1139, 2677, 2941
BccI CCATC 5 cut(s) 634, 764, 1631, 1813, 2819
BceAI ACGGC 5 cut(s) 279, 884, 1770, 1777, 2561
BcgI CGANNNNNNTGC 2 cut(s) 2792, 2826
BciT130I CCWGG 3 cut(s) 178, 2973, 3348
BciVI GTATCC 1 cut(s) 2530
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 3 cut(s) 1132, 1660, 1872
BcoDI GTCTC 8 cut(s) 131, 254, 275, 730, 838, 1089, 1624, 1806
BfaI CTAG 7 cut(s) 587, 896, 1055, 2019, 2121, 2657, 3273
BfmI CTRYAG 2 cut(s) 2472, 2759
BfoI RGCGCY 2 cut(s) 349, 1911
BfuI GTATCC 1 cut(s) 2530
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 486, 591, 1128, 2691, 2955
BlsI GCNGC 5 cut(s) 487, 592, 1129, 2692, 2956
Bme1390I CCNGG 6 cut(s) 178, 1132, 1660, 1872, 2973, 3348
Bme18I GGWCC 3 cut(s) 180, 2559, 3344
BmeT110I CYCGRG 2 cut(s) 2704, 3106
BmgT120I GGNCC 5 cut(s) 180, 1874, 2559, 3283, 3344
BmiI GGNNCC 4 cut(s) 1351, 1620, 1875, 1981
BmrFI CCNGG 6 cut(s) 178, 1132, 1660, 1872, 2973, 3348
BmsI GCATC 3 cut(s) 862, 2722, 2869
BmtI GCTAGC 1 cut(s) 2124
BpiI GAAGAC 6 cut(s) 858, 2455, 2821, 2990, 3057, 3269
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 2 cut(s) 1220, 2415
BpuEI CTTGAG 2 cut(s) 1123, 3251
BpuMI CCSGG 3 cut(s) 1132, 1660, 1872
Bsa29I ATCGAT 1 cut(s) 2340
BsaAI YACGTR 5 cut(s) 1123, 1366, 1471, 1576, 2840
BsaJI CCNNGG 7 cut(s) 489, 1622, 1659, 1765, 1871, 1975, 3316
BsaWI WCCGGW 1 cut(s) 1963
Bsc4I CCNNNNNNNGG 4 cut(s) 495, 1137, 2068, 3317
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 2 cut(s) 1021, 1898
Bse3DI GCAATG 1 cut(s) 2392
BseBI CCWGG 3 cut(s) 178, 2973, 3348
BseCI ATCGAT 1 cut(s) 2340
BseDI CCNNGG 7 cut(s) 489, 1622, 1659, 1765, 1871, 1975, 3316
BseGI GGATG 4 cut(s) 775, 1153, 1632, 2884
BseLI CCNNNNNNNGG 4 cut(s) 495, 1137, 2068, 3317
BseMI GCAATG 1 cut(s) 2392
BseMII CTCAG 1 cut(s) 2298
BseNI ACTGG 2 cut(s) 1021, 1898
BseSI GKGCMC 1 cut(s) 501
BseXI GCAGC 4 cut(s) 602, 1139, 2677, 2941
BsgI GTGCAG 3 cut(s) 653, 1092, 3198
Bsh1236I CGCG 4 cut(s) 79, 503, 505, 1817
BshFI GGCC 9 cut(s) 176, 266, 488, 1764, 1785, 1876, 1923, 2072, 3284
BshNI GGYRCC 1 cut(s) 1618
BshVI ATCGAT 1 cut(s) 2340
BsiHKAI GWGCWC 3 cut(s) 237, 1792, 2932
BsiHKCI CYCGRG 2 cut(s) 2704, 3106
BsiSI CCGG 6 cut(s) 629, 1132, 1659, 1871, 1964, 3281
BslFI GGGAC 3 cut(s) 401, 902, 2057
BslI CCNNNNNNNGG 4 cut(s) 495, 1137, 2068, 3317
BsmAI GTCTC 8 cut(s) 131, 254, 275, 730, 838, 1089, 1624, 1806
BsmBI CGTCTC 5 cut(s) 254, 275, 1089, 1624, 1806
BsmFI GGGAC 3 cut(s) 401, 902, 2057
BsmI GAATGC 2 cut(s) 196, 1866
BsnI GGCC 9 cut(s) 176, 266, 488, 1764, 1785, 1876, 1923, 2072, 3284
BsoBI CYCGRG 2 cut(s) 2704, 3106
Bsp1286I GDGCHC 4 cut(s) 237, 501, 1792, 2932
Bsp1407I TGTACA 2 cut(s) 1702, 2915
Bsp19I CCATGG 2 cut(s) 489, 1622
BspACI CCGC 5 cut(s) 79, 424, 485, 1212, 2334
BspANI GGCC 9 cut(s) 176, 266, 488, 1764, 1785, 1876, 1923, 2072, 3284
BspCNI CTCAG 1 cut(s) 2297
BspDI ATCGAT 1 cut(s) 2340
BspFNI CGCG 4 cut(s) 79, 503, 505, 1817
BspHI TCATGA 3 cut(s) 1416, 1521, 2257
BspLI GGNNCC 4 cut(s) 1351, 1620, 1875, 1981
BspOI GCTAGC 1 cut(s) 2124
BspPI GGATC 5 cut(s) 1055, 1082, 1974, 1987, 2384
BspQI GCTCTTC 7 cut(s) 242, 480, 585, 789, 894, 1122, 1917
BspT107I GGYRCC 1 cut(s) 1618
BsrDI GCAATG 1 cut(s) 2392
BsrFI RCCGGY 1 cut(s) 628
BsrGI TGTACA 2 cut(s) 1702, 2915
BsrI ACTGG 2 cut(s) 1021, 1898
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 7 cut(s) 489, 1622, 1659, 1765, 1871, 1975, 3316
BssNAI GTATAC 1 cut(s) 2189
BssT1I CCWWGG 4 cut(s) 489, 1622, 1765, 3316
Bst1107I GTATAC 1 cut(s) 2189
Bst2UI CCWGG 3 cut(s) 178, 2973, 3348
Bst4CI ACNGT 8 cut(s) 933, 996, 1081, 1553, 2080, 2186, 2763, 2920
BstAPI GCANNNNNTGC 1 cut(s) 1597
BstAUI TGTACA 2 cut(s) 1702, 2915
BstBAI YACGTR 5 cut(s) 1123, 1366, 1471, 1576, 2840
BstC8I GCNNGC 4 cut(s) 501, 1386, 2122, 2126
BstDEI CTNAG 3 cut(s) 407, 433, 2284
BstDSI CCRYGG 3 cut(s) 489, 1622, 1975
BstF5I GGATG 4 cut(s) 775, 1153, 1632, 2884
BstFNI CGCG 4 cut(s) 79, 503, 505, 1817
BstH2I RGCGCY 2 cut(s) 349, 1911
BstHHI GCGC 4 cut(s) 348, 505, 1390, 1910
BstMAI GTCTC 8 cut(s) 131, 254, 275, 730, 838, 1089, 1624, 1806
BstMWI GCNNNNNNNGC 5 cut(s) 206, 272, 692, 1180, 1597
BstNI CCWGG 3 cut(s) 178, 2973, 3348
BstNSI RCATGY 5 cut(s) 608, 1145, 1388, 1703, 2128
BstSCI CCNGG 6 cut(s) 176, 1130, 1658, 1870, 2971, 3346
BstSFI CTRYAG 2 cut(s) 2472, 2759
BstSLI GKGCMC 1 cut(s) 501
BstUI CGCG 4 cut(s) 79, 503, 505, 1817
BstV1I GCAGC 4 cut(s) 602, 1139, 2677, 2941
BstV2I GAAGAC 6 cut(s) 858, 2455, 2821, 2990, 3057, 3269
BstX2I RGATCY 4 cut(s) 106, 1087, 1979, 2376
BstYI RGATCY 4 cut(s) 106, 1087, 1979, 2376
BstZ17I GTATAC 1 cut(s) 2189
Bsu15I ATCGAT 1 cut(s) 2340
BsuI GTATCC 1 cut(s) 2530
BsuRI GGCC 9 cut(s) 176, 266, 488, 1764, 1785, 1876, 1923, 2072, 3284
BsuTUI ATCGAT 1 cut(s) 2340
BtgI CCRYGG 3 cut(s) 489, 1622, 1975
BtgZI GCGATG 1 cut(s) 1832
BtsCI GGATG 4 cut(s) 775, 1153, 1632, 2884
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 433, 2664, 2768
Cac8I GCNNGC 4 cut(s) 501, 1386, 2122, 2126
CciI TCATGA 3 cut(s) 1416, 1521, 2257
CfoI GCGC 4 cut(s) 348, 505, 1390, 1910
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 5 cut(s) 180, 1874, 2559, 3283, 3344
ClaI ATCGAT 1 cut(s) 2340
CseI GACGC 2 cut(s) 544, 1823
Csp6I GTAC 5 cut(s) 205, 650, 1619, 1703, 2916
CviQI GTAC 5 cut(s) 205, 650, 1619, 1703, 2916
DdeI CTNAG 3 cut(s) 407, 433, 2284
EaeI YGGCCR 4 cut(s) 264, 486, 1762, 2070
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 4 cut(s) 489, 1622, 1765, 3316
Eco147I AGGCCT 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 3 cut(s) 817, 1943, 2224
Eco47I GGWCC 3 cut(s) 180, 2559, 3344
Eco47III AGCGCT 2 cut(s) 347, 1909
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 123, 1236
Eco72I CACGTG 1 cut(s) 2840
Eco88I CYCGRG 2 cut(s) 2704, 3106
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 1874
EcoRI GAATTC 2 cut(s) 2293, 3096
EcoRII CCWGG 3 cut(s) 176, 2971, 3346
EcoRV GATATC 3 cut(s) 817, 1943, 2224
EcoT14I CCWWGG 4 cut(s) 489, 1622, 1765, 3316
EcoT22I ATGCAT 1 cut(s) 2805
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 4 cut(s) 489, 1622, 1765, 3316
Esp3I CGTCTC 5 cut(s) 254, 275, 1089, 1624, 1806
FalI AAGNNNNNCTT 4 cut(s) 1029, 1061, 1254, 1286
FaqI GGGAC 3 cut(s) 401, 902, 2057
FauNDI CATATG 2 cut(s) 2355, 2713
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 2 cut(s) 2188, 3335
Fnu4HI GCNGC 5 cut(s) 486, 591, 1128, 2691, 2955
FokI GGATG 4 cut(s) 782, 1160, 1639, 2891
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 486, 591, 1128, 2691, 2955
FspAI RTGCGCAY 1 cut(s) 1389
FspBI CTAG 7 cut(s) 587, 896, 1055, 2019, 2121, 2657, 3273
FspI TGCGCA 1 cut(s) 1389
GlaI GCGC 4 cut(s) 347, 504, 1389, 1909
GluI GCNGC 5 cut(s) 486, 591, 1128, 2691, 2955
GsuI CTGGAG 2 cut(s) 1220, 2415
HaeII RGCGCY 2 cut(s) 349, 1911
HaeIII GGCC 9 cut(s) 176, 266, 488, 1764, 1785, 1876, 1923, 2072, 3284
HapII CCGG 6 cut(s) 629, 1132, 1659, 1871, 1964, 3281
HgaI GACGC 2 cut(s) 544, 1823
HhaI GCGC 4 cut(s) 348, 505, 1390, 1910
Hin6I GCGC 4 cut(s) 346, 503, 1388, 1908
HinP1I GCGC 4 cut(s) 346, 503, 1388, 1908
HincII GTYRAC 1 cut(s) 2212
HindII GTYRAC 1 cut(s) 2212
HindIII AAGCTT 1 cut(s) 1743
HpaI GTTAAC 1 cut(s) 2212
HpaII CCGG 6 cut(s) 629, 1132, 1659, 1871, 1964, 3281
Hpy166II GTNNAC 8 cut(s) 691, 999, 2083, 2189, 2212, 2662, 2843, 3336
Hpy188I TCNGA 7 cut(s) 134, 334, 365, 801, 2641, 2824, 3046
Hpy8I GTNNAC 8 cut(s) 691, 999, 2083, 2189, 2212, 2662, 2843, 3336
HpyAV CCTTC 6 cut(s) 190, 488, 686, 694, 1421, 1526
HpyCH4III ACNGT 8 cut(s) 933, 996, 1081, 1553, 2080, 2186, 2763, 2920
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 272, 692, 1180, 1597
HpyF3I CTNAG 3 cut(s) 407, 433, 2284
HspAI GCGC 4 cut(s) 346, 503, 1388, 1908
KpnI GGTACC 1 cut(s) 1622
Ksp22I TGATCA 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 2212
LguI GCTCTTC 7 cut(s) 242, 480, 585, 789, 894, 1122, 1917
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 4 cut(s) 602, 1139, 2677, 2941
LweI GCATC 3 cut(s) 862, 2722, 2869
MaeI CTAG 7 cut(s) 587, 896, 1055, 2019, 2121, 2657, 3273
MflI RGATCY 4 cut(s) 106, 1087, 1979, 2376
MhlI GDGCHC 4 cut(s) 237, 501, 1792, 2932
MlsI TGGCCA 1 cut(s) 2072
MluNI TGGCCA 1 cut(s) 2072
MlyI GAGTC 3 cut(s) 133, 2148, 2851
MmeI TCCRAC 2 cut(s) 267, 357
Mox20I TGGCCA 1 cut(s) 2072
Mph1103I ATGCAT 1 cut(s) 2805
MroXI GAANNNNTTC 3 cut(s) 9, 2308, 2818
MscI TGGCCA 1 cut(s) 2072
MseI TTAA 7 cut(s) 759, 1047, 2141, 2211, 2451, 2769, 3026
MslI CAYNNNNRTG 7 cut(s) 609, 1007, 1146, 2256, 2371, 2823, 3194
Msp20I TGGCCA 1 cut(s) 2072
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 6 cut(s) 629, 1132, 1659, 1871, 1964, 3281
MspR9I CCNGG 6 cut(s) 178, 1132, 1660, 1872, 2973, 3348
Mva1269I GAATGC 2 cut(s) 196, 1866
MvaI CCWGG 3 cut(s) 178, 2973, 3348
MvnI CGCG 4 cut(s) 79, 503, 505, 1817
MwoI GCNNNNNNNGC 5 cut(s) 206, 272, 692, 1180, 1597
NciI CCSGG 3 cut(s) 1132, 1660, 1872
NcoI CCATGG 2 cut(s) 489, 1622
NdeI CATATG 2 cut(s) 2355, 2713
NheI GCTAGC 1 cut(s) 2120
NlaIV GGNNCC 4 cut(s) 1351, 1620, 1875, 1981
NmuCI GTSAC 4 cut(s) 569, 665, 1009, 3187
NsbI TGCGCA 1 cut(s) 1389
NsiI ATGCAT 1 cut(s) 2805
NspI RCATGY 5 cut(s) 608, 1145, 1388, 1703, 2128
OliI CACNNNNGTG 1 cut(s) 1007
PaeI GCATGC 2 cut(s) 1388, 2128
PaeR7I CTCGAG 1 cut(s) 3106
PagI TCATGA 3 cut(s) 1416, 1521, 2257
PceI AGGCCT 1 cut(s) 176
PciI ACATGT 1 cut(s) 1699
PciSI GCTCTTC 7 cut(s) 242, 480, 585, 789, 894, 1122, 1917
PcsI WCGNNNNNNNCGW 1 cut(s) 2158
PctI GAATGC 2 cut(s) 196, 1866
PdmI GAANNNNTTC 3 cut(s) 9, 2308, 2818
PfeI GAWTC 7 cut(s) 563, 1457, 1562, 1655, 1849, 2243, 2337
PkrI GCNGC 5 cut(s) 487, 592, 1129, 2692, 2956
PleI GAGTC 3 cut(s) 133, 2148, 2851
PmaCI CACGTG 1 cut(s) 2840
PmlI CACGTG 1 cut(s) 2840
PpsI GAGTC 3 cut(s) 133, 2148, 2851
Ppu21I YACGTR 5 cut(s) 1123, 1366, 1471, 1576, 2840
PscI ACATGT 1 cut(s) 1699
PsiI TTATAA 1 cut(s) 1344
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 2056
Psp6I CCWGG 3 cut(s) 176, 2971, 3346
PspCI CACGTG 1 cut(s) 2840
PspGI CCWGG 3 cut(s) 176, 2971, 3346
PspN4I GGNNCC 4 cut(s) 1351, 1620, 1875, 1981
PspPI GGNCC 5 cut(s) 180, 1874, 2559, 3283, 3344
PspXI VCTCGAGB 1 cut(s) 3106
PsuI RGATCY 4 cut(s) 106, 1087, 1979, 2376
RsaI GTAC 5 cut(s) 206, 651, 1620, 1704, 2917
RsaNI GTAC 5 cut(s) 205, 650, 1619, 1703, 2916
RseI CAYNNNNRTG 7 cut(s) 609, 1007, 1146, 2256, 2371, 2823, 3194
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 7 cut(s) 242, 480, 585, 789, 894, 1122, 1917
SaqAI TTAA 7 cut(s) 759, 1047, 2141, 2211, 2451, 2769, 3026
SatI GCNGC 5 cut(s) 486, 591, 1128, 2691, 2955
Sau96I GGNCC 5 cut(s) 180, 1874, 2559, 3283, 3344
SchI GAGTC 3 cut(s) 133, 2148, 2851
ScrFI CCNGG 6 cut(s) 178, 1132, 1660, 1872, 2973, 3348
SduI GDGCHC 4 cut(s) 237, 501, 1792, 2932
SfaNI GCATC 3 cut(s) 862, 2722, 2869
SfcI CTRYAG 2 cut(s) 2472, 2759
Sfr274I CTCGAG 1 cut(s) 3106
SinI GGWCC 3 cut(s) 180, 2559, 3344
SlaI CTCGAG 1 cut(s) 3106
SmiMI CAYNNNNRTG 7 cut(s) 609, 1007, 1146, 2256, 2371, 2823, 3194
SmlI CTYRAG 3 cut(s) 1102, 3106, 3230
SmoI CTYRAG 3 cut(s) 1102, 3106, 3230
SphI GCATGC 2 cut(s) 1388, 2128
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 5 cut(s) 79, 424, 485, 1212, 2334
SspI AATATT 1 cut(s) 964
SspMI CTAG 7 cut(s) 587, 896, 1055, 2019, 2121, 2657, 3273
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 6 cut(s) 176, 1130, 1658, 1870, 2971, 3346
StyI CCWWGG 4 cut(s) 489, 1622, 1765, 3316
TaaI ACNGT 8 cut(s) 933, 996, 1081, 1553, 2080, 2186, 2763, 2920
TatI WGTACW 3 cut(s) 649, 1702, 2915
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 7 cut(s) 563, 1457, 1562, 1655, 1849, 2243, 2337
Tru1I TTAA 7 cut(s) 759, 1047, 2141, 2211, 2451, 2769, 3026
Tru9I TTAA 7 cut(s) 759, 1047, 2141, 2211, 2451, 2769, 3026
TscAI CASTG 3 cut(s) 433, 2671, 2768
TseFI GTSAC 4 cut(s) 569, 665, 1009, 3187
TseI GCWGC 4 cut(s) 590, 1127, 2690, 2954
Tsp45I GTSAC 4 cut(s) 569, 665, 1009, 3187
TspGWI ACGGA 5 cut(s) 468, 472, 477, 1992, 2549
TspRI CASTG 3 cut(s) 433, 2671, 2768
VpaK11BI GGWCC 3 cut(s) 180, 2559, 3344
XapI RAATTY 4 cut(s) 217, 2293, 2327, 3096
XceI RCATGY 5 cut(s) 608, 1145, 1388, 1703, 2128
XcmI CCANNNNNNNNNTGG 2 cut(s) 221, 1982
XhoI CTCGAG 1 cut(s) 3106
XmiI GTMKAC 2 cut(s) 2188, 3335
XmnI GAANNNNTTC 3 cut(s) 9, 2308, 2818
XspI CTAG 7 cut(s) 587, 896, 1055, 2019, 2121, 2657, 3273
Zsp2I ATGCAT 1 cut(s) 2805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.