Rroxscaffold_4G00323300

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000952
Physical Location & Seq
Reverse (-)
53983286 .. 53984465
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00323300.1

Sequence Viewer

Length: 723 bp
ATGGAGCAGAAGAAGGAGAAAGAGGCTGTGGAGGATCTGCAACGATTTCCGAAAAGAAGCCTCGCCGCCCGTTGTTCCGCCACCGTCACCATCACTCTCCCCACCTTCAACGATCTCTGCCGCCGATTCCCAACTGTCCAATGCGTCCCTATCGCAGCTGTGAAGCCTTTTTCCGGCTATACTCGGTTCACCACTTATGTGGCAAATGGTGATTGGGATAATGCAAAAGAGTGCCTTACAAAACTAGATGATCCATGTGACGCAATAATGATAGCGGATCCAAGAGATGGATATGGAGACACCGCTCTTCATGTAGCAGCTAGAAAAGGGCATGTGCATATTGTGAAAGAGTTGTTATTGTTGCTGAAAGTGAGACAAGAAAATTTGGAACTGAAAACCATAGAAGATTTCACGGCTTTTGGTTCTGGTATAAATTTCGGAGTCAATGAACAGGTTGTCATGGAAAGGGCCAAATACATGGTTGAACAGTATGTGAAAATGCTTACCAATATTTTAGGTGTACAAAATGCAAAAGGTTTCACAGCCCTTCATCTAGCAGTATTGAAGCGGAATTTGGATGTTGTGAAAGAGTTGGTGCCATTCATGGGAGAAGAAGGTCTAGAAATAAAGGACGCCGATGGAGACACGACTCTTCACATCTCGATCTTACGGGGGCAGCTACACTTAATTTCCTTGACTGTGGACGATGAATTCTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.94

Weight (kDa)

5.73

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank PF00023 98 - 119 6e-06 Ankyrin repeat
Ank_2 PF12796 165 - 230 1.5e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 175 - 220 5.6e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 305
AciI CCGC 6 cut(s) 66, 78, 121, 275, 303, 568
AclWI GGATC 4 cut(s) 42, 245, 272, 285
AcsI RAATTY 4 cut(s) 382, 433, 571, 710
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 1 cut(s) 522
AfiI CCNNNNNNNGG 3 cut(s) 173, 287, 605
AgsI TTSAA 3 cut(s) 109, 485, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 3 cut(s) 158, 320, 679
AluI AGCT 3 cut(s) 158, 320, 679
Alw26I GTCTC 3 cut(s) 291, 367, 636
AlwI GGATC 4 cut(s) 42, 245, 272, 285
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 3 cut(s) 155, 317, 676
ApoI RAATTY 4 cut(s) 382, 433, 571, 710
AspS9I GGNCC 1 cut(s) 468
AsuHPI GGTGA 3 cut(s) 79, 181, 221
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 595
BbvI GCAGC 3 cut(s) 167, 329, 688
BccI CCATC 3 cut(s) 98, 281, 632
BceAI ACGGC 1 cut(s) 429
BcoDI GTCTC 3 cut(s) 291, 367, 636
BfaI CTAG 4 cut(s) 245, 321, 554, 620
BisI GCNGC 5 cut(s) 66, 121, 156, 318, 677
BlsI GCNGC 5 cut(s) 67, 122, 157, 319, 678
BmgT120I GGNCC 1 cut(s) 468
BmiI GGNNCC 2 cut(s) 279, 597
BsaHI GRCGYC 1 cut(s) 633
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 287, 605
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 3 cut(s) 173, 287, 605
BseXI GCAGC 3 cut(s) 167, 329, 688
BshFI GGCC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 3 cut(s) 173, 287, 605
BsmAI GTCTC 3 cut(s) 291, 367, 636
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 470
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 5 cut(s) 34, 112, 250, 277, 663
BspACI CCGC 6 cut(s) 66, 78, 121, 275, 303, 568
BspANI GGCC 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 279, 597
BspPI GGATC 4 cut(s) 42, 245, 272, 285
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 595
BsrBI CCGCTC 1 cut(s) 305
BsrGI TGTACA 1 cut(s) 520
BssMI GATC 5 cut(s) 34, 112, 250, 277, 663
BssNI GRCGYC 1 cut(s) 633
Bst4CI ACNGT 4 cut(s) 85, 136, 489, 700
Bst6I CTCTTC 2 cut(s) 312, 657
BstACI GRCGYC 1 cut(s) 633
BstAUI TGTACA 1 cut(s) 520
BstF5I GGATG 1 cut(s) 583
BstKTI GATC 5 cut(s) 37, 115, 253, 280, 666
BstMAI GTCTC 3 cut(s) 291, 367, 636
BstMBI GATC 5 cut(s) 34, 112, 250, 277, 663
BstNSI RCATGY 1 cut(s) 335
BstV1I GCAGC 3 cut(s) 167, 329, 688
BstX2I RGATCY 2 cut(s) 34, 277
BstXI CCANNNNNNTGG 2 cut(s) 199, 478
BstYI RGATCY 2 cut(s) 34, 277
BsuRI GGCC 1 cut(s) 470
BtsCI GGATG 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 468
CseI GACGC 3 cut(s) 133, 269, 641
Csp6I GTAC 1 cut(s) 521
CviAII CATG 6 cut(s) 255, 311, 332, 460, 478, 604
CviQI GTAC 1 cut(s) 521
DpnI GATC 5 cut(s) 36, 114, 252, 279, 665
DpnII GATC 5 cut(s) 34, 112, 250, 277, 663
Eam1104I CTCTTC 2 cut(s) 312, 657
EarI CTCTTC 2 cut(s) 312, 657
EciI GGCGGA 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 710
FaeI CATG 6 cut(s) 258, 314, 335, 463, 481, 607
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 1 cut(s) 131
FatI CATG 6 cut(s) 254, 310, 331, 459, 477, 603
Fnu4HI GCNGC 5 cut(s) 66, 121, 156, 318, 677
FokI GGATG 1 cut(s) 590
Fsp4HI GCNGC 5 cut(s) 66, 121, 156, 318, 677
FspBI CTAG 4 cut(s) 245, 321, 554, 620
GluI GCNGC 5 cut(s) 66, 121, 156, 318, 677
HaeIII GGCC 1 cut(s) 470
HapII CCGG 1 cut(s) 174
HgaI GACGC 3 cut(s) 133, 269, 641
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 6 cut(s) 258, 314, 335, 463, 481, 607
HinfI GANTC 3 cut(s) 126, 441, 649
HpaII CCGG 1 cut(s) 174
HphI GGTGA 3 cut(s) 79, 181, 221
Hpy166II GTNNAC 3 cut(s) 189, 521, 703
Hpy188I TCNGA 2 cut(s) 51, 440
Hpy188III TCNNGA 2 cut(s) 620, 661
Hpy8I GTNNAC 3 cut(s) 189, 521, 703
HpyAV CCTTC 4 cut(s) 7, 115, 557, 608
HpyCH4III ACNGT 4 cut(s) 85, 136, 489, 700
HpyCH4V TGCA 4 cut(s) 40, 224, 337, 530
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 6 cut(s) 258, 314, 335, 463, 481, 607
Kzo9I GATC 5 cut(s) 34, 112, 250, 277, 663
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 3 cut(s) 187, 411, 437
Lsp1109I GCAGC 3 cut(s) 167, 329, 688
MaeI CTAG 4 cut(s) 245, 321, 554, 620
MaeIII GTNAC 2 cut(s) 85, 257
MalI GATC 5 cut(s) 36, 114, 252, 279, 665
MbiI CCGCTC 1 cut(s) 305
MboI GATC 5 cut(s) 34, 112, 250, 277, 663
MboII GAAGA 6 cut(s) 22, 299, 416, 623, 644, 706
MflI RGATCY 2 cut(s) 34, 277
MluCI AATT 5 cut(s) 382, 433, 571, 687, 710
MlyI GAGTC 2 cut(s) 450, 643
MnlI CCTC 3 cut(s) 16, 25, 71
MseI TTAA 1 cut(s) 686
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 1 cut(s) 174
NdeII GATC 5 cut(s) 34, 112, 250, 277, 663
NlaIII CATG 6 cut(s) 258, 314, 335, 463, 481, 607
NlaIV GGNNCC 2 cut(s) 279, 597
NmuCI GTSAC 2 cut(s) 85, 257
NspI RCATGY 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 5 cut(s) 67, 122, 157, 319, 678
PleI GAGTC 2 cut(s) 449, 643
PpsI GAGTC 2 cut(s) 449, 643
PspN4I GGNNCC 2 cut(s) 279, 597
PspPI GGNCC 1 cut(s) 468
PsuI RGATCY 2 cut(s) 34, 277
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 1 cut(s) 522
RsaNI GTAC 1 cut(s) 521
RseI CAYNNNNRTG 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 686
SatI GCNGC 5 cut(s) 66, 121, 156, 318, 677
Sau3AI GATC 5 cut(s) 34, 112, 250, 277, 663
Sau96I GGNCC 1 cut(s) 468
SchI GAGTC 2 cut(s) 450, 643
SetI ASST 8 cut(s) 107, 160, 322, 456, 520, 538, 619, 681
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 5 cut(s) 382, 433, 571, 687, 710
SsiI CCGC 6 cut(s) 66, 78, 121, 275, 303, 568
SspI AATATT 1 cut(s) 511
SspMI CTAG 4 cut(s) 245, 321, 554, 620
TaaI ACNGT 4 cut(s) 85, 136, 489, 700
TaqI TCGA 1 cut(s) 662
TasI AATT 5 cut(s) 382, 433, 571, 687, 710
TatI WGTACW 1 cut(s) 520
TauI GCSGC 2 cut(s) 68, 123
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 686
Tru9I TTAA 1 cut(s) 686
TseFI GTSAC 2 cut(s) 85, 257
TseI GCWGC 3 cut(s) 155, 317, 676
Tsp45I GTSAC 2 cut(s) 85, 257
TspDTI ATGAA 5 cut(s) 299, 462, 539, 592, 723
Van91I CCANNNNNTGG 1 cut(s) 287
XapI RAATTY 4 cut(s) 382, 433, 571, 710
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 335
XspI CTAG 4 cut(s) 245, 321, 554, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.