Rh7BG444000

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000952
Physical Location & Seq
Forward (+)
51633437 .. 51634472
1036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG444000.1

Sequence Viewer

Length: 786 bp
ATGGCAAGTACTCCAGTTGGCTCTAATGCACTTATTTTGGAAGTTCTTGACCGTAATAACTATAAACATTGGAGATCGAGGTTGGAAACTTATTTGTTGGCTGAAGATCTCTGGGAGGTTGTTGAAGCAATCCCGCCAAAACTCGAAGATGATCAAGCCGAATATAAGGCTTGGACCAAAAAGAATGCAAAGGCTTTGTATGCAATCCAAAATTCATGCGGGAGGGAGCTGTTCTCTTTCATAAGTGAAATTAAGACGGCAAAAGGTGCTTGGGAGGCTTTGGAAGCAGTGCAAAGATATGATGAGAACAATACCGACCACTTAGTGCGCTATAAATCGTTCATAGAATCAGTGAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATGTCCGTAGTGTATTAGTAGTAAAAACAGTATACAGCTATGGCGGAGAAATAGCTCTTCATGTAGCAGCCGAGGAAGGGCATGCACATATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTGAAAACAAAAACCGATTTTGGTCACACAGCTCTTCACCTAGCAGCAACGAAGGGGCATGTGTATGTTGTGAAAGAGTTACCGGTGCAGTTGATGGGAGAAGTACAAGACTGGGGAGGTGACACAGCTCTTCATATAGCAGTCCAGGAAGGGAAGGTCGATGTTGTTAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACTTGAAAATTAAAAACGATGCTGGTTACACAGCTCTTCACCTAGCAGTACGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

261

Amino Acids

29.37

Weight (kDa)

5.68

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2.2e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 97 2.6e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 148 - 203 2.9e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 211 - 258 5.6e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 214 - 260 3.7e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 432
AciI CCGC 3 cut(s) 134, 219, 444
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 3 cut(s) 10, 633, 777
AfiI CCNNNNNNNGG 1 cut(s) 477
AgeI ACCGGT 1 cut(s) 610
AgsI TTSAA 3 cut(s) 125, 532, 733
AjnI CCWGG 1 cut(s) 672
AluBI AGCT 6 cut(s) 229, 438, 455, 560, 656, 761
AluI AGCT 6 cut(s) 229, 438, 455, 560, 656, 761
Alw26I GTCTC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 467, 572
ApoI RAATTY 1 cut(s) 211
AsiGI ACCGGT 1 cut(s) 610
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 4 cut(s) 374, 557, 659, 758
AvaII GGWCC 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 732
BbvI GCAGC 2 cut(s) 479, 584
BccI CCATC 1 cut(s) 616
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 674
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 712
BfaI CTAG 2 cut(s) 569, 770
BglII AGATCT 1 cut(s) 106
BisI GCNGC 2 cut(s) 468, 573
BlsI GCNGC 2 cut(s) 469, 574
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 674
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 674
BmrI ACTGGG 1 cut(s) 649
BmsI GCATC 1 cut(s) 736
BmuI ACTGGG 1 cut(s) 649
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BsaJI CCNNGG 1 cut(s) 471
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 2 cut(s) 14, 644
BseBI CCWGG 1 cut(s) 674
BseDI CCNNGG 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 477
BseNI ACTGG 2 cut(s) 14, 644
BseXI GCAGC 2 cut(s) 479, 584
BsgI GTGCAG 1 cut(s) 635
BshTI ACCGGT 1 cut(s) 610
BsiSI CCGG 1 cut(s) 611
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 712
BsmI GAATGC 1 cut(s) 190
Bsp143I GATC 3 cut(s) 74, 106, 151
BspACI CCGC 3 cut(s) 134, 219, 444
BspQI GCTCTTC 4 cut(s) 462, 567, 663, 768
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 2 cut(s) 14, 644
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 3 cut(s) 74, 106, 151
BssNAI GTATAC 1 cut(s) 433
Bst1107I GTATAC 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 674
Bst4CI ACNGT 2 cut(s) 53, 430
Bst6I CTCTTC 4 cut(s) 462, 567, 663, 768
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 1 cut(s) 322
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 3 cut(s) 77, 109, 154
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 3 cut(s) 74, 106, 151
BstMWI GCNNNNNNNGC 4 cut(s) 200, 266, 275, 284
BstNI CCWGG 1 cut(s) 674
BstNSI RCATGY 2 cut(s) 485, 590
BstSCI CCNGG 1 cut(s) 672
BstV1I GCAGC 2 cut(s) 479, 584
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BstZ17I GTATAC 1 cut(s) 433
BtsI GCAGTG 2 cut(s) 294, 364
BtsIMutI CAGTG 3 cut(s) 294, 357, 364
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 3 cut(s) 9, 632, 776
CspAI ACCGGT 1 cut(s) 610
CviAII CATG 4 cut(s) 216, 461, 482, 587
CviQI GTAC 3 cut(s) 9, 632, 776
DdeI CTNAG 1 cut(s) 322
DpnI GATC 3 cut(s) 76, 108, 153
DpnII GATC 3 cut(s) 74, 106, 151
DraIII CACNNNGTG 1 cut(s) 325
Eam1104I CTCTTC 4 cut(s) 462, 567, 663, 768
EarI CTCTTC 4 cut(s) 462, 567, 663, 768
EciI GGCGGA 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 672
FaeI CATG 4 cut(s) 219, 464, 485, 590
FatI CATG 4 cut(s) 215, 460, 481, 586
FauI CCCGC 2 cut(s) 141, 212
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 468, 573
Fsp4HI GCNGC 2 cut(s) 468, 573
FspBI CTAG 2 cut(s) 569, 770
GlaI GCGC 1 cut(s) 329
GluI GCNGC 2 cut(s) 468, 573
HapII CCGG 1 cut(s) 611
HhaI GCGC 1 cut(s) 330
Hin1II CATG 4 cut(s) 219, 464, 485, 590
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HinfI GANTC 1 cut(s) 347
HpaII CCGG 1 cut(s) 611
HphI GGTGA 4 cut(s) 374, 557, 659, 758
Hpy166II GTNNAC 1 cut(s) 433
Hpy188III TCNNGA 2 cut(s) 47, 493
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 4 cut(s) 470, 574, 671, 676
HpyCH4III ACNGT 2 cut(s) 53, 430
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 7 cut(s) 29, 188, 203, 292, 485, 508, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 266, 275, 284
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 1 cut(s) 778
Hsp92II CATG 4 cut(s) 219, 464, 485, 590
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 74, 106, 151
LguI GCTCTTC 4 cut(s) 462, 567, 663, 768
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 7 cut(s) 27, 97, 624, 625, 659, 686, 735
Lsp1109I GCAGC 2 cut(s) 479, 584
LweI GCATC 1 cut(s) 736
MaeI CTAG 2 cut(s) 569, 770
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 4 cut(s) 551, 606, 647, 752
MalI GATC 3 cut(s) 76, 108, 153
MboI GATC 3 cut(s) 74, 106, 151
MboII GAAGA 8 cut(s) 116, 158, 449, 536, 554, 650, 737, 755
MflI RGATCY 1 cut(s) 106
MluCI AATT 3 cut(s) 211, 249, 735
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 6 cut(s) 72, 109, 216, 268, 466, 638
MseI TTAA 3 cut(s) 252, 696, 738
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 1 cut(s) 674
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 674
MwoI GCNNNNNNNGC 4 cut(s) 200, 266, 275, 284
NdeII GATC 3 cut(s) 74, 106, 151
NlaIII CATG 4 cut(s) 219, 464, 485, 590
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 2 cut(s) 551, 647
NspI RCATGY 2 cut(s) 485, 590
PaeI GCATGC 1 cut(s) 485
PciSI GCTCTTC 4 cut(s) 462, 567, 663, 768
PctI GAATGC 1 cut(s) 190
PfeI GAWTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 672
PinAI ACCGGT 1 cut(s) 610
PkrI GCNGC 2 cut(s) 469, 574
Psp6I CCWGG 1 cut(s) 672
PspGI CCWGG 1 cut(s) 672
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 3 cut(s) 10, 633, 777
RsaNI GTAC 3 cut(s) 9, 632, 776
RseI CAYNNNNRTG 1 cut(s) 591
SapI GCTCTTC 4 cut(s) 462, 567, 663, 768
SaqAI TTAA 3 cut(s) 252, 696, 738
SatI GCNGC 2 cut(s) 468, 573
Sau3AI GATC 3 cut(s) 74, 106, 151
Sau96I GGNCC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 674
SfaNI GCATC 1 cut(s) 736
SinI GGWCC 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 591
SphI GCATGC 1 cut(s) 485
Sse9I AATT 3 cut(s) 211, 249, 735
SsiI CCGC 3 cut(s) 134, 219, 444
SspMI CTAG 2 cut(s) 569, 770
StyD4I CCNGG 1 cut(s) 672
TaaI ACNGT 2 cut(s) 53, 430
TaiI ACGT 1 cut(s) 781
TaqI TCGA 3 cut(s) 77, 144, 687
TasI AATT 3 cut(s) 211, 249, 735
TatI WGTACW 2 cut(s) 8, 631
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 3 cut(s) 252, 696, 738
Tru9I TTAA 3 cut(s) 252, 696, 738
TscAI CASTG 3 cut(s) 294, 357, 364
TseFI GTSAC 2 cut(s) 551, 647
TseI GCWGC 2 cut(s) 467, 572
Tsp45I GTSAC 2 cut(s) 551, 647
TspDTI ATGAA 5 cut(s) 204, 229, 331, 449, 650
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 3 cut(s) 294, 357, 364
VpaK11BI GGWCC 1 cut(s) 174
XapI RAATTY 1 cut(s) 211
XceI RCATGY 2 cut(s) 485, 590
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 569, 770
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.