FvH4_5g36330

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000952
Physical Location & Seq
Forward (+)
26658088 .. 26658994
907 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36330.t1

Sequence Viewer

Length: 717 bp
ATGAAAAAGGCCAAATACATGGTTGAACAGTACGAGAACACACTTGGCAGTTGTTTGGAGATACAAAACGCTACAGGTTCCACAGCTCTTCGCCAAGCAGTACTGCAGGGGAATGTGGATGTTGTGAAAGAGTTGGTGCCGTTTATGAGAAAAGAAGGTCTAGAAATACAAGACGCGGATGGAGACACACCTATCAACTGTGCACTATTAAGTGGGAATGTGGGTATTGCCAAGGAGTTAGTACAATCCATGAGACAAGAAGAGGCTTTGGAAGACAAAGATGCTCAAGGTGTTACAGCTTTAAATTGGGCAATCCAATGTTTCAAGGATGGAGACGTTTACGAAATAGCTAAAAAGATGGCTGGAATAAATGATCAAGTACTTGGCATTCCCTCGGGTCGCGATGATGGTATTCCCGTTGTCAACGCACTTCGTTGGAAAAAATGGGAATCGGTTCGCTATCTCTATTCAGTCACTCCACCTGAACTTCTCAACGGACCCAATGGCAGTGCCTTTATTTGCTATTGTTTAGATAATAGCAAACAGAATGATTTGGCTTGGGACTTGCTTCGCCATTACCCATACTTGGCTATGACTATGCACGAAAAGCTTAAAACTTCTCCTATGTTGAAATTGTCTCGCATGAGATATGCCTTCTTAAGCGGAAGCCAACTGACGCGCTGGCAAAGATGGATTTATAACCGTGAGTATAGCTAA

Protein Analysis

239

Amino Acids

26.98

Weight (kDa)

6.18

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 82 7.3e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 136
AccII CGCG 3 cut(s) 176, 402, 679
AciI CCGC 2 cut(s) 176, 663
AfaI GTAC 4 cut(s) 32, 102, 243, 381
AfiI CCNNNNNNNGG 1 cut(s) 586
AflII CTTAAG 1 cut(s) 658
AgsI TTSAA 3 cut(s) 26, 325, 631
AluBI AGCT 5 cut(s) 86, 299, 350, 610, 714
AluI AGCT 5 cut(s) 86, 299, 350, 610, 714
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 4 cut(s) 177, 247, 327, 642
Alw44I GTGCAC 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 394
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 201
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 497
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 1 cut(s) 497
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 205
BccI CCATC 5 cut(s) 173, 323, 352, 401, 684
BceAI ACGGC 1 cut(s) 124
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 177, 247, 327, 642
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 2 cut(s) 72, 104
BfrI CTTAAG 1 cut(s) 658
BmcAI AGTACT 2 cut(s) 102, 381
Bme18I GGWCC 1 cut(s) 497
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 3 cut(s) 79, 138, 499
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 231, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 586
BseDI CCNNGG 2 cut(s) 231, 393
BseGI GGATG 3 cut(s) 124, 184, 334
BseLI CCNNNNNNNGG 1 cut(s) 586
BseSI GKGCMC 1 cut(s) 205
Bsh1236I CGCG 3 cut(s) 176, 402, 679
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 394
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 4 cut(s) 177, 247, 327, 642
BsmBI CGTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 1 cut(s) 373
Bsp68I TCGCGA 1 cut(s) 402
BspACI CCGC 2 cut(s) 176, 663
BspANI GGCC 1 cut(s) 11
BspFNI CGCG 3 cut(s) 176, 402, 679
BspLI GGNNCC 3 cut(s) 79, 138, 499
BspMAI CTGCAG 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 136
BspTI CTTAAG 1 cut(s) 658
BssECI CCNNGG 2 cut(s) 231, 393
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 231
Bst4CI ACNGT 3 cut(s) 30, 200, 704
Bst6I CTCTTC 2 cut(s) 93, 255
BstAFI CTTAAG 1 cut(s) 658
BstC8I GCNNGC 1 cut(s) 683
BstF5I GGATG 3 cut(s) 124, 184, 334
BstFNI CGCG 3 cut(s) 176, 402, 679
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 4 cut(s) 177, 247, 327, 642
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstSFI CTRYAG 2 cut(s) 72, 104
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 3 cut(s) 176, 402, 679
BstV2I GAAGAC 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 19
BsuRI GGCC 1 cut(s) 11
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 124, 184, 334
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 514
BtuMI TCGCGA 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 683
CfoI GCGC 1 cut(s) 681
Cfr13I GGNCC 1 cut(s) 497
CseI GACGC 2 cut(s) 182, 685
Csp6I GTAC 4 cut(s) 31, 101, 242, 380
CviAII CATG 3 cut(s) 19, 250, 643
CviQI GTAC 4 cut(s) 31, 101, 242, 380
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
DraI TTTAAA 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 93, 255
EarI CTCTTC 2 cut(s) 93, 255
Eco130I CCWWGG 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 3 cut(s) 22, 253, 646
FaqI GGGAC 1 cut(s) 575
FatI CATG 3 cut(s) 18, 249, 642
FbaI TGATCA 1 cut(s) 373
FokI GGATG 3 cut(s) 131, 191, 341
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 680
HaeIII GGCC 1 cut(s) 11
HgaI GACGC 2 cut(s) 182, 685
HhaI GCGC 1 cut(s) 681
Hin1II CATG 3 cut(s) 22, 253, 646
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 1 cut(s) 449
Hpy166II GTNNAC 3 cut(s) 203, 340, 424
Hpy188III TCNNGA 2 cut(s) 161, 401
Hpy8I GTNNAC 3 cut(s) 203, 340, 424
HpyAV CCTTC 2 cut(s) 149, 664
HpyCH4III ACNGT 3 cut(s) 30, 200, 704
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 3 cut(s) 106, 203, 601
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 3 cut(s) 22, 253, 646
HspAI GCGC 1 cut(s) 679
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 93
LpnPI CCDG 5 cut(s) 60, 92, 348, 495, 667
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 292, 472
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 3 cut(s) 80, 272, 284
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 2 cut(s) 304, 632
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 2 cut(s) 256, 403
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 4 cut(s) 209, 302, 612, 659
MspCI CTTAAG 1 cut(s) 658
Mva1269I GAATGC 1 cut(s) 387
MvnI CGCG 3 cut(s) 176, 402, 679
MwoI GCNNNNNNNGC 1 cut(s) 607
NdeII GATC 1 cut(s) 373
NlaIII CATG 3 cut(s) 22, 253, 646
NlaIV GGNNCC 3 cut(s) 79, 138, 499
NmuCI GTSAC 1 cut(s) 472
NruI TCGCGA 1 cut(s) 402
PciSI GCTCTTC 1 cut(s) 93
PctI GAATGC 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 449
PsiI TTATAA 1 cut(s) 699
PspN4I GGNNCC 3 cut(s) 79, 138, 499
PspPI GGNCC 1 cut(s) 497
PstI CTGCAG 1 cut(s) 108
RruI TCGCGA 1 cut(s) 402
RsaI GTAC 4 cut(s) 32, 102, 243, 381
RsaNI GTAC 4 cut(s) 31, 101, 242, 380
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 4 cut(s) 209, 302, 612, 659
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 497
ScaI AGTACT 2 cut(s) 102, 381
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 2 cut(s) 72, 104
SinI GGWCC 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 285, 658
SmoI CTYRAG 2 cut(s) 285, 658
Sse9I AATT 2 cut(s) 304, 632
SsiI CCGC 2 cut(s) 176, 663
SspMI CTAG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 3 cut(s) 30, 200, 704
TaiI ACGT 1 cut(s) 339
TasI AATT 2 cut(s) 304, 632
TatI WGTACW 3 cut(s) 100, 241, 379
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 4 cut(s) 209, 302, 612, 659
Tru9I TTAA 4 cut(s) 209, 302, 612, 659
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 1 cut(s) 514
Vha464I CTTAAG 1 cut(s) 658
VneI GTGCAC 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 497
XbaI TCTAGA 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 1 cut(s) 161
ZrmI AGTACT 2 cut(s) 102, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.