FvH4_5g36641

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000952
Physical Location & Seq
Reverse (-)
26877074 .. 26882365
5292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36641.t2

Sequence Viewer

Length: 1398 bp
ATGCACGTAGCAGCCTGGAAAGGGCACGTGAATATCGTGAAAGAGTTGATGATGTTGATGACCCACAAAGATTTGAAAATGGGAGACGCTAGAGGTAATACAGCTCTTCATGTAGCAGCTGGTAATGGGCAACTACAGACTGTGAAAGAGTTGGTGTTGTTGATGGGAGAAAAAATATACAACGACGGTGACACAGCACTTCACGTAGCAGTCCAATGGGAGGAGGTGGACGTTGTGAAAGGGCTGGTGTCGTTAATGAGACAAACAGACTTGAAAATAAAAAACAACAATGGTGACACAGCTCTTCACCTAGCAGTCAAGAAGAGAAATGTGGCTATGATGAAATATTTAGCGGCGTTGATTGGAGAAGTGAGAGGCCCTGATGGTGACACTGCTCTTCACCGAGCAGTCAAGTTGGAGGATGCAAAAGTTGTGAAAGAGTTGGTGGTATTGATGAAAGAGGAAGATTTGGCAATAGAAAATGATGAAGGTTACACCGCTCTTCACTTGGCAGTAAAGAAGGGTATTGTACCTGTTGTGAAAGAGTTCATGACAAAAGAATTAAAAGAGCGCGAGTTTCATAGTGATATTCAGTTCACTACTTATGTAACAAAAGGTGAGTGGGATAATGCAAAGGAGTATCTTACAAAACTAGATGAACCTTGTAACGCAATAACGATAGTAGATCCAAGAGACGGATATGGAAACACTGCTCTACATGTAGCAGCAGCTAGAGAAGGGCACGCACATATTGTGAAAGAGTTGATATCATTGCTGAAAGTGAGACAAGAAATTCAGGAATGGCAAACTATAGAAGATATCGCAGCTTTTGGTTCTGCTATAAATTTAGGGGTCACTGAAGAAGTTGTCAGGAAAAAGGCCAAGAACATGGTCGAGCAGTACGATAAATTGCTTACCAGTATTTTGGAGGTACAAAATGCATATGGTGCCACAGCCGTTCACATAGCAGTAGCCAACGGTAATTTAGATACTGTGAAAGAGTTGGTGCCATTAATGAGAAAAGAAGGTTTAGAGATACAAGATGCTTACGGATTCACAGCTCTTCTCCGTGCATCCATTGGTAATTTAGATATTGTGAAAGAGTTGGTGCCAATAATGAGAAAAGAAGGTTTAGAAATACAAAATTATGAAGGACGCACAGCTCTTCTCAATGCAGTCGCATGGGGTCATATTGATATTGTGAAAGAGTTGTTGCCACGAAGGTCTGGAACTAAAAGCAGCCAAAGATGGCTGCAACGCACTTCACCTTGCGGTCGTGAAAGGGCATATGAATATTGCGAGAGAGGTGATGCCATTTATGAGAAAAGAGGCTTTGGAAGAAAAAGACGGTGCAGATTTTACGGCTTTAGGTTGTGCGCTAGCTGTACGCAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

466

Amino Acids

52.11

Weight (kDa)

7.66

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 1 - 84 5.2e-15 Ankyrin repeats (3 copies)
Ank_2 PF12796 87 - 149 1.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 127 - 184 2.2e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 308 - 370 5.9e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 346 - 406 9.5e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 947, 1006, 1108
AccBSI CCGCTC 1 cut(s) 500
AccII CGCG 1 cut(s) 573
AciI CCGC 3 cut(s) 353, 498, 1272
AclWI GGATC 1 cut(s) 680
AcsI RAATTY 2 cut(s) 792, 844
AcuI CTGAAG 1 cut(s) 879
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 4 cut(s) 531, 902, 933, 1387
AfiI CCNNNNNNNGG 3 cut(s) 21, 220, 695
AflIII ACRYGT 1 cut(s) 718
AgsI TTSAA 2 cut(s) 76, 274
AjnI CCWGG 1 cut(s) 14
AluBI AGCT 8 cut(s) 104, 119, 302, 731, 827, 1061, 1163, 1383
AluI AGCT 8 cut(s) 104, 119, 302, 731, 827, 1061, 1163, 1383
Alw26I GTCTC 4 cut(s) 78, 253, 687, 778
AlwI GGATC 1 cut(s) 680
AoxI GGCC 2 cut(s) 376, 879
ApeKI GCWGC 7 cut(s) 11, 116, 725, 728, 824, 1239, 1252
ApoI RAATTY 2 cut(s) 792, 844
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 2 cut(s) 573, 1379
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 8 cut(s) 200, 299, 305, 392, 398, 629, 1257, 1319
AsuNHI GCTAGC 1 cut(s) 1379
BaeGI GKGCMC 2 cut(s) 27, 744
BanI GGYRCC 3 cut(s) 947, 1006, 1108
BbrPI CACGTG 1 cut(s) 28
BbvI GCAGC 7 cut(s) 23, 128, 737, 740, 836, 1239, 1251
BccI CCATC 3 cut(s) 157, 377, 1242
BceAI ACGGC 2 cut(s) 941, 1378
BciT130I CCWGG 1 cut(s) 16
BcoDI GTCTC 4 cut(s) 78, 253, 687, 778
BfaI CTAG 5 cut(s) 90, 311, 653, 732, 1380
BfmI CTRYAG 2 cut(s) 134, 810
BisI GCNGC 8 cut(s) 12, 117, 354, 726, 729, 825, 1240, 1253
BlsI GCNGC 8 cut(s) 13, 118, 355, 727, 730, 826, 1241, 1254
Bme1390I CCNGG 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 3 cut(s) 949, 1008, 1110
BmrFI CCNGG 1 cut(s) 16
BmsI GCATC 4 cut(s) 412, 1033, 1082, 1300
BmtI GCTAGC 1 cut(s) 1383
BsaAI YACGTR 3 cut(s) 7, 28, 205
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 220, 695
Bse1I ACTGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 770
BseBI CCWGG 1 cut(s) 16
BseGI GGATG 2 cut(s) 427, 1073
BseLI CCNNNNNNNGG 3 cut(s) 21, 220, 695
BseMI GCAATG 1 cut(s) 770
BseNI ACTGG 1 cut(s) 918
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 2 cut(s) 27, 744
BseXI GCAGC 7 cut(s) 23, 128, 737, 740, 836, 1239, 1251
BsgI GTGCAG 1 cut(s) 1372
Bsh1236I CGCG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 1276
BshFI GGCC 2 cut(s) 378, 881
BshNI GGYRCC 3 cut(s) 947, 1006, 1108
BsiEI CGRYCG 1 cut(s) 1276
BslI CCNNNNNNNGG 3 cut(s) 21, 220, 695
BsmAI GTCTC 4 cut(s) 78, 253, 687, 778
BsmBI CGTCTC 2 cut(s) 78, 687
BsnI GGCC 2 cut(s) 378, 881
Bsp1286I GDGCHC 2 cut(s) 27, 744
Bsp143I GATC 1 cut(s) 685
BspACI CCGC 3 cut(s) 353, 498, 1272
BspANI GGCC 2 cut(s) 378, 881
BspFNI CGCG 1 cut(s) 573
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 949, 1008, 1110
BspOI GCTAGC 1 cut(s) 1383
BspPI GGATC 1 cut(s) 680
BspQI GCTCTTC 6 cut(s) 111, 309, 402, 507, 1068, 1170
BspT107I GGYRCC 3 cut(s) 947, 1006, 1108
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 770
BsrI ACTGG 1 cut(s) 918
BssMI GATC 1 cut(s) 685
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 5 cut(s) 142, 188, 980, 994, 1350
Bst6I CTCTTC 7 cut(s) 111, 309, 317, 402, 507, 1068, 1170
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBAI YACGTR 3 cut(s) 7, 28, 205
BstC8I GCNNGC 2 cut(s) 744, 1381
BstF5I GGATG 2 cut(s) 427, 1073
BstFNI CGCG 1 cut(s) 573
BstHHI GCGC 2 cut(s) 573, 1379
BstKTI GATC 1 cut(s) 688
BstMAI GTCTC 4 cut(s) 78, 253, 687, 778
BstMBI GATC 1 cut(s) 685
BstMCI CGRYCG 1 cut(s) 1276
BstMWI GCNNNNNNNGC 1 cut(s) 947
BstNI CCWGG 1 cut(s) 16
BstNSI RCATGY 1 cut(s) 722
BstSCI CCNGG 1 cut(s) 14
BstSFI CTRYAG 2 cut(s) 134, 810
BstSLI GKGCMC 2 cut(s) 27, 744
BstUI CGCG 1 cut(s) 573
BstV1I GCAGC 7 cut(s) 23, 128, 737, 740, 836, 1239, 1251
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 2 cut(s) 889, 925
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 2 cut(s) 378, 881
BtsCI GGATG 2 cut(s) 427, 1073
BtsI GCAGTG 2 cut(s) 390, 708
BtsIMutI CAGTG 3 cut(s) 390, 708, 855
Cac8I GCNNGC 2 cut(s) 744, 1381
CciI TCATGA 1 cut(s) 549
CfoI GCGC 2 cut(s) 573, 1379
Cfr13I GGNCC 1 cut(s) 377
CseI GACGC 2 cut(s) 95, 1164
Csp6I GTAC 4 cut(s) 530, 901, 932, 1386
CviAII CATG 5 cut(s) 110, 550, 719, 889, 1182
CviQI GTAC 4 cut(s) 530, 901, 932, 1386
DpnI GATC 1 cut(s) 687
DpnII GATC 1 cut(s) 685
Eam1104I CTCTTC 7 cut(s) 111, 309, 317, 402, 507, 1068, 1170
EarI CTCTTC 7 cut(s) 111, 309, 317, 402, 507, 1068, 1170
Eco32I GATATC 2 cut(s) 768, 820
Eco57I CTGAAG 1 cut(s) 879
Eco72I CACGTG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 14
EcoRV GATATC 2 cut(s) 768, 820
EcoT22I ATGCAT 1 cut(s) 943
Esp3I CGTCTC 2 cut(s) 78, 687
FaeI CATG 5 cut(s) 113, 553, 722, 892, 1185
FatI CATG 5 cut(s) 109, 549, 718, 888, 1181
FauNDI CATATG 2 cut(s) 943, 1288
Fnu4HI GCNGC 8 cut(s) 12, 117, 354, 726, 729, 825, 1240, 1253
FokI GGATG 2 cut(s) 434, 1060
Fsp4HI GCNGC 8 cut(s) 12, 117, 354, 726, 729, 825, 1240, 1253
FspBI CTAG 5 cut(s) 90, 311, 653, 732, 1380
GlaI GCGC 2 cut(s) 572, 1378
GluI GCNGC 8 cut(s) 12, 117, 354, 726, 729, 825, 1240, 1253
HaeIII GGCC 2 cut(s) 378, 881
HgaI GACGC 2 cut(s) 95, 1164
HhaI GCGC 2 cut(s) 573, 1379
Hin1II CATG 5 cut(s) 113, 553, 722, 892, 1185
Hin6I GCGC 2 cut(s) 571, 1377
HinP1I GCGC 2 cut(s) 571, 1377
HinfI GANTC 1 cut(s) 1053
HphI GGTGA 8 cut(s) 200, 299, 305, 392, 398, 629, 1257, 1319
Hpy166II GTNNAC 3 cut(s) 229, 597, 961
Hpy188III TCNNGA 7 cut(s) 37, 319, 550, 797, 871, 1227, 1277
Hpy8I GTNNAC 3 cut(s) 229, 597, 961
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 7 cut(s) 482, 514, 731, 1019, 1121, 1145, 1215
HpyCH4III ACNGT 5 cut(s) 142, 188, 980, 994, 1350
HpyCH4IV ACGT 4 cut(s) 6, 27, 204, 231
HpyCH4V TGCA 8 cut(s) 4, 425, 632, 941, 1073, 1175, 1255, 1353
HpyF10VI GCNNNNNNNGC 1 cut(s) 947
HpySE526I ACGT 4 cut(s) 6, 27, 204, 231
Hsp92II CATG 5 cut(s) 113, 553, 722, 892, 1185
HspAI GCGC 2 cut(s) 571, 1377
Kzo9I GATC 1 cut(s) 685
LguI GCTCTTC 6 cut(s) 111, 309, 402, 507, 1068, 1170
LpnPI CCDG 9 cut(s) 28, 105, 230, 393, 546, 782, 856, 931, 1212
Lsp1109I GCAGC 7 cut(s) 23, 128, 737, 740, 836, 1239, 1251
LweI GCATC 4 cut(s) 412, 1033, 1082, 1300
MaeI CTAG 5 cut(s) 90, 311, 653, 732, 1380
MaeII ACGT 4 cut(s) 6, 27, 204, 231
MaeIII GTNAC 7 cut(s) 188, 293, 386, 491, 607, 665, 853
MalI GATC 1 cut(s) 687
MbiI CCGCTC 1 cut(s) 500
MboI GATC 1 cut(s) 685
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 27, 744
MluCI AATT 7 cut(s) 560, 792, 844, 908, 982, 1084, 1144
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 9 cut(s) 86, 214, 217, 368, 412, 454, 922, 1298, 1322
Mph1103I ATGCAT 1 cut(s) 943
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 3 cut(s) 254, 563, 1013
MspA1I CMGCKG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 16
MvaI CCWGG 1 cut(s) 16
MvnI CGCG 1 cut(s) 573
MwoI GCNNNNNNNGC 1 cut(s) 947
NdeI CATATG 2 cut(s) 943, 1288
NdeII GATC 1 cut(s) 685
NheI GCTAGC 1 cut(s) 1379
NlaIII CATG 5 cut(s) 113, 553, 722, 892, 1185
NlaIV GGNNCC 3 cut(s) 949, 1008, 1110
NmuCI GTSAC 4 cut(s) 188, 293, 386, 853
NsiI ATGCAT 1 cut(s) 943
NspI RCATGY 1 cut(s) 722
PagI TCATGA 1 cut(s) 549
PciI ACATGT 1 cut(s) 718
PciSI GCTCTTC 6 cut(s) 111, 309, 402, 507, 1068, 1170
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 900
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 1 cut(s) 1053
PkrI GCNGC 8 cut(s) 13, 118, 355, 727, 730, 826, 1241, 1254
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 3 cut(s) 7, 28, 205
PscI ACATGT 1 cut(s) 718
PshBI ATTAAT 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 14
PspCI CACGTG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 949, 1008, 1110
PspPI GGNCC 1 cut(s) 377
PsuI RGATCY 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 4 cut(s) 531, 902, 933, 1387
RsaNI GTAC 4 cut(s) 530, 901, 932, 1386
SapI GCTCTTC 6 cut(s) 111, 309, 402, 507, 1068, 1170
SaqAI TTAA 3 cut(s) 254, 563, 1013
SatI GCNGC 8 cut(s) 12, 117, 354, 726, 729, 825, 1240, 1253
Sau3AI GATC 1 cut(s) 685
Sau96I GGNCC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 2 cut(s) 27, 744
SfaNI GCATC 4 cut(s) 412, 1033, 1082, 1300
SfcI CTRYAG 2 cut(s) 134, 810
Sse9I AATT 7 cut(s) 560, 792, 844, 908, 982, 1084, 1144
SsiI CCGC 3 cut(s) 353, 498, 1272
SspI AATATT 2 cut(s) 347, 1295
SspMI CTAG 5 cut(s) 90, 311, 653, 732, 1380
StyD4I CCNGG 1 cut(s) 14
TaaI ACNGT 5 cut(s) 142, 188, 980, 994, 1350
TaiI ACGT 4 cut(s) 9, 30, 207, 234
TaqI TCGA 1 cut(s) 894
TasI AATT 7 cut(s) 560, 792, 844, 908, 982, 1084, 1144
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 1053
Tru1I TTAA 3 cut(s) 254, 563, 1013
Tru9I TTAA 3 cut(s) 254, 563, 1013
TscAI CASTG 3 cut(s) 397, 715, 862
TseFI GTSAC 4 cut(s) 188, 293, 386, 853
TseI GCWGC 7 cut(s) 11, 116, 725, 728, 824, 1239, 1252
Tsp45I GTSAC 4 cut(s) 188, 293, 386, 853
TspDTI ATGAA 9 cut(s) 98, 356, 470, 501, 538, 569, 672, 1164, 1305
TspGWI ACGGA 3 cut(s) 711, 1058, 1065
TspRI CASTG 3 cut(s) 397, 715, 862
VspI ATTAAT 1 cut(s) 1013
XapI RAATTY 2 cut(s) 792, 844
XceI RCATGY 1 cut(s) 722
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 5 cut(s) 90, 311, 653, 732, 1380
Zsp2I ATGCAT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.