Rw7G039390

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000952
Physical Location & Seq
Forward (+)
60659638 .. 60662077
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039390.1

Sequence Viewer

Length: 1734 bp
ATGAATACTCCAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTGAAAACTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCGAAGCAATCGTGAAACCTCCAAAACGTGAAGATGATCAAACTGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTGTACGCAATCCAAAATACATGTGGGAGAGAGCTGTTCTCTTTTATAAGGGAAATTGAGACGGCCAAAAGTGCCTGGGAGGCTTTGGAGCTAGAGTGCAAAGTTATGCAAGGATATGATGAGAACAATGTGGACCACTTGGGGCGCTATAAATCGTTCATAGAATCAGTGAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATGCGCAGTGTAGTAGTAAGGGCAGTAGATGGAGATGGAGAAATAGCTCTTCATGTAGCAGCCAAGGAAGGGCATGCGAACATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTGAAAACGAAAACCGCTCATGGTCACACAGCTCTTCACCTAGCAGCAGGGGAGGGGCATGTGCATGTTGTGAAAGAGTTACCGGTGCCATTGATGGGAGAAGTACAAGACTGGGAAGGTGACACAGCTCTTCATATCGCAGTCCAGGAAGGGAAGGTCGATATTGTGAAAGAGTTGGTGGTGTTGATGAGACAAGAAGACTTGAAAATAAAAAACGATGCTGGTTACACAGCTCTGCACCTAGCAGTCAATAAGGGGAATGTGCCTATGGTCAAAGAGTTCGTGAGAAAACAAAAAGGTGAGAATAATATTGGCGAGGACGACTTTGAGCGTTATATACCGTTTACCACCTATGTGCAAAATGGTGATTGGGATAAGGTAAATGAGTGTCTTAGAGAACTAGATAATCCTTATGATGCAGTAACAGCAGTAGATCCAAGAGACTACCACCGAGACACTGCTCTTCACGTAGCAGCCCGGGAAGGGCATGTGGATGTTACCAAAGGATTGGTGTTGTTGCTGAAAATGAGACAAGAAGATTTGGAATTGAAAACCGCTGAAGATTTCACAACTTTTTGTTCTGGTATAACTTTAGGAGTGAGTGAAGAATTTCTCATGAAAATGGCTAAATACATGGTTGAACAGTTCGAGAAAACACGTGGCAGCTTTTTGGAGATACAAAATGCTACAGGTTCCATAGCTCTTCACCTAGCAGTATTGAATGGGCATGTAGGTATTGTGAAAGAGTTTGTGCCATTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGACGGAGACACAGCTCTTAACATTGCAATCTTACAAGGGTGTGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACCAGAGGCTTTGGAAGAAAAAGACGGTCAAGGTTTTACAGCTTTAAGTAAGGCTATTCAATGTTTCAAGGATGGAGACATTTGCGAAATGGCTAAATACATGGCTGAAAAAAATGTTAAAGTATTTGGCACACCCTCGACTGCCCATGATGAAATTCCAGTTGTCACAGCTCTTCGTCAGGCTAATTGGAACTTGGCTCGCTACATGTATTCTGTCACTCCACTTGAAGCTCTCGAAGGACCAAATGGCAGTTCACTTATTTGCTTATGTCTCAATACCAGCGAAACGATTGGTAAGACTACTTTCAGTTTTAATAGTAAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

577

Amino Acids

64.47

Weight (kDa)

5.17

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 15 - 41 1.9e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 28 - 96 5.3e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 146 - 206 7.5e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 148 - 194 6.1e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 214 - 261 4.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 216 - 276 4.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 402 - 466 5.6e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 414 - 509 5.1e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 168, 245
Acc16I TGCGCA 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 622
AccBSI CCGCTC 1 cut(s) 554
AccII CGCG 1 cut(s) 76
AciI CCGC 2 cut(s) 552, 1092
AclWI GGATC 1 cut(s) 965
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 2 cut(s) 1145, 1560
AcuI CTGAAG 2 cut(s) 120, 1116
AcvI CACGTG 1 cut(s) 1196
AfaI GTAC 2 cut(s) 203, 642
AfiI CCNNNNNNNGG 3 cut(s) 486, 632, 1020
AflIII ACRYGT 3 cut(s) 218, 1193, 1611
AgeI ACCGGT 1 cut(s) 619
AgsI TTSAA 9 cut(s) 82, 541, 742, 1087, 1178, 1258, 1466, 1474, 1634
AjnI CCWGG 3 cut(s) 173, 272, 681
AjuI GAANNNNNNNTTGG 2 cut(s) 1642, 1674
AleI CACNNNNGTG 1 cut(s) 890
Alw26I GTCTC 9 cut(s) 251, 721, 972, 984, 1060, 1329, 1399, 1476, 1682
AlwI GGATC 1 cut(s) 965
Ama87I CYCGRG 1 cut(s) 1014
AoxI GGCC 2 cut(s) 171, 261
ApeKI GCWGC 4 cut(s) 476, 581, 1010, 1200
ApoI RAATTY 2 cut(s) 1145, 1560
AseI ATTAAT 1 cut(s) 1295
AsiGI ACCGGT 1 cut(s) 619
Asp700I GAANNNNTTC 1 cut(s) 1146
AspLEI GCGC 2 cut(s) 345, 423
AspS9I GGNCC 3 cut(s) 177, 331, 1646
AsuC2I CCSGG 2 cut(s) 1015, 1016
AsuHPI GGTGA 6 cut(s) 389, 566, 668, 848, 914, 1235
AvaI CYCGRG 1 cut(s) 1014
AvaII GGWCC 3 cut(s) 177, 331, 1646
BanI GGYRCC 1 cut(s) 622
BbrPI CACGTG 1 cut(s) 1196
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 4 cut(s) 488, 593, 1022, 1212
BccI CCATC 4 cut(s) 440, 446, 625, 1472
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 3 cut(s) 175, 274, 683
BclI TGATCA 1 cut(s) 154
BcnI CCSGG 2 cut(s) 1015, 1016
BcoDI GTCTC 9 cut(s) 251, 721, 972, 984, 1060, 1329, 1399, 1476, 1682
BfaI CTAG 5 cut(s) 290, 578, 779, 938, 1247
BfmI CTRYAG 1 cut(s) 1224
BfoI RGCGCY 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 1 cut(s) 103
BisI GCNGC 4 cut(s) 477, 582, 1011, 1201
BlsI GCNGC 4 cut(s) 478, 583, 1012, 1202
Bme1390I CCNGG 5 cut(s) 175, 274, 683, 1015, 1016
Bme18I GGWCC 3 cut(s) 177, 331, 1646
BmeT110I CYCGRG 1 cut(s) 1014
BmgT120I GGNCC 3 cut(s) 177, 331, 1646
BmiI GGNNCC 2 cut(s) 624, 1231
BmrFI CCNGG 5 cut(s) 175, 274, 683, 1015, 1016
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 2 cut(s) 745, 943
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpuMI CCSGG 2 cut(s) 1015, 1016
Bsa29I ATCGAT 1 cut(s) 1400
BsaAI YACGTR 2 cut(s) 1006, 1196
BsaI GGTCTC 1 cut(s) 1399
BsaJI CCNNGG 4 cut(s) 192, 273, 480, 1014
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 632, 1020
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 3 cut(s) 11, 653, 1565
Bse3DI GCAATG 1 cut(s) 1350
BseBI CCWGG 3 cut(s) 175, 274, 683
BseCI ATCGAT 1 cut(s) 1400
BseDI CCNNGG 4 cut(s) 192, 273, 480, 1014
BseGI GGATG 2 cut(s) 1036, 1483
BseLI CCNNNNNNNGG 3 cut(s) 486, 632, 1020
BseMI GCAATG 1 cut(s) 1350
BseNI ACTGG 3 cut(s) 11, 653, 1565
BseXI GCAGC 4 cut(s) 488, 593, 1022, 1212
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 2 cut(s) 173, 263
BshNI GGYRCC 1 cut(s) 622
BshTI ACCGGT 1 cut(s) 619
BshVI ATCGAT 1 cut(s) 1400
BsiHKCI CYCGRG 1 cut(s) 1014
BsiSI CCGG 2 cut(s) 620, 1015
BslI CCNNNNNNNGG 3 cut(s) 486, 632, 1020
BsmAI GTCTC 9 cut(s) 251, 721, 972, 984, 1060, 1329, 1399, 1476, 1682
BsmBI CGTCTC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 2 cut(s) 173, 263
Bso31I GGTCTC 1 cut(s) 1399
BsoBI CYCGRG 1 cut(s) 1014
Bsp143I GATC 4 cut(s) 71, 103, 154, 970
Bsp68I TCGCGA 1 cut(s) 76
BspACI CCGC 2 cut(s) 552, 1092
BspANI GGCC 2 cut(s) 173, 263
BspDI ATCGAT 1 cut(s) 1400
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 2 cut(s) 624, 1231
BspPI GGATC 1 cut(s) 965
BspQI GCTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
BspT107I GGYRCC 1 cut(s) 622
BspTNI GGTCTC 1 cut(s) 1399
BsrBI CCGCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 1350
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 3 cut(s) 11, 653, 1565
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 4 cut(s) 192, 273, 480, 1014
BssMI GATC 4 cut(s) 71, 103, 154, 970
BssT1I CCWWGG 2 cut(s) 192, 480
Bst2UI CCWGG 3 cut(s) 175, 274, 683
Bst4CI ACNGT 4 cut(s) 50, 879, 1182, 1433
Bst6I CTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
BstBAI YACGTR 2 cut(s) 1006, 1196
BstC8I GCNNGC 2 cut(s) 492, 1606
BstDEI CTNAG 1 cut(s) 929
BstF5I GGATG 2 cut(s) 1036, 1483
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 345, 423
BstKTI GATC 4 cut(s) 74, 106, 157, 973
BstMAI GTCTC 9 cut(s) 251, 721, 972, 984, 1060, 1329, 1399, 1476, 1682
BstMBI GATC 4 cut(s) 71, 103, 154, 970
BstMWI GCNNNNNNNGC 5 cut(s) 122, 203, 269, 278, 962
BstNI CCWGG 3 cut(s) 175, 274, 683
BstNSI RCATGY 7 cut(s) 222, 494, 599, 605, 1028, 1268, 1615
BstSCI CCNGG 5 cut(s) 173, 272, 681, 1013, 1014
BstSFI CTRYAG 1 cut(s) 1224
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 488, 593, 1022, 1212
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 103, 970
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 2 cut(s) 103, 970
Bsu15I ATCGAT 1 cut(s) 1400
BsuRI GGCC 2 cut(s) 173, 263
BsuTUI ATCGAT 1 cut(s) 1400
BtsCI GGATG 2 cut(s) 1036, 1483
BtsI GCAGTG 3 cut(s) 379, 430, 993
BtsIMutI CAGTG 4 cut(s) 372, 379, 430, 993
BtuMI TCGCGA 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 492, 1606
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 2 cut(s) 345, 423
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 3 cut(s) 177, 331, 1646
Cfr9I CCCGGG 1 cut(s) 1014
ClaI ATCGAT 1 cut(s) 1400
CseI GACGC 1 cut(s) 1334
Csp6I GTAC 2 cut(s) 202, 641
CspAI ACCGGT 1 cut(s) 619
CviQI GTAC 2 cut(s) 202, 641
DdeI CTNAG 1 cut(s) 929
DpnI GATC 4 cut(s) 73, 105, 156, 972
DpnII GATC 4 cut(s) 71, 103, 154, 970
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
EarI CTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
Eco130I CCWWGG 2 cut(s) 192, 480
Eco147I AGGCCT 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 1399
Eco47I GGWCC 3 cut(s) 177, 331, 1646
Eco57I CTGAAG 2 cut(s) 120, 1116
Eco72I CACGTG 1 cut(s) 1196
Eco88I CYCGRG 1 cut(s) 1014
EcoRII CCWGG 3 cut(s) 173, 272, 681
EcoT14I CCWWGG 2 cut(s) 192, 480
ErhI CCWWGG 2 cut(s) 192, 480
Esp3I CGTCTC 1 cut(s) 251
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 4 cut(s) 477, 582, 1011, 1201
FokI GGATG 2 cut(s) 1043, 1490
Fsp4HI GCNGC 4 cut(s) 477, 582, 1011, 1201
FspBI CTAG 5 cut(s) 290, 578, 779, 938, 1247
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 2 cut(s) 344, 422
GluI GCNGC 4 cut(s) 477, 582, 1011, 1201
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 2 cut(s) 173, 263
HapII CCGG 2 cut(s) 620, 1015
HgaI GACGC 1 cut(s) 1334
HhaI GCGC 2 cut(s) 345, 423
Hin6I GCGC 2 cut(s) 343, 421
HinP1I GCGC 2 cut(s) 343, 421
HinfI GANTC 1 cut(s) 362
HpaII CCGG 2 cut(s) 620, 1015
HphI GGTGA 6 cut(s) 389, 566, 668, 848, 914, 1235
Hpy166II GTNNAC 3 cut(s) 331, 882, 1661
Hpy188III TCNNGA 9 cut(s) 35, 44, 75, 130, 502, 820, 1153, 1186, 1640
Hpy8I GTNNAC 3 cut(s) 331, 882, 1661
HpyAV CCTTC 8 cut(s) 479, 647, 680, 685, 1013, 1301, 1378, 1637
HpyCH4III ACNGT 4 cut(s) 50, 879, 1182, 1433
HpyCH4IV ACGT 3 cut(s) 145, 1005, 1195
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 203, 269, 278, 962
HpyF3I CTNAG 1 cut(s) 929
HpySE526I ACGT 3 cut(s) 145, 1005, 1195
HspAI GCGC 2 cut(s) 343, 421
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 4 cut(s) 71, 103, 154, 970
LguI GCTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 4 cut(s) 488, 593, 1022, 1212
LweI GCATC 2 cut(s) 745, 943
MaeI CTAG 5 cut(s) 290, 578, 779, 938, 1247
MaeII ACGT 3 cut(s) 145, 1005, 1195
MaeIII GTNAC 9 cut(s) 53, 560, 615, 656, 761, 958, 1033, 1570, 1621
MalI GATC 4 cut(s) 73, 105, 156, 972
MbiI CCGCTC 1 cut(s) 554
MboI GATC 4 cut(s) 71, 103, 154, 970
MflI RGATCY 2 cut(s) 103, 970
MluCI AATT 5 cut(s) 252, 1082, 1145, 1560, 1591
MnlI CCTC 7 cut(s) 106, 147, 271, 583, 847, 1405, 1552
MroXI GAANNNNTTC 1 cut(s) 1146
MseI TTAA 5 cut(s) 1295, 1347, 1451, 1524, 1719
MslI CAYNNNNRTG 4 cut(s) 600, 890, 1029, 1157
MspA1I CMGCKG 1 cut(s) 1094
MspI CCGG 2 cut(s) 620, 1015
MspR9I CCNGG 5 cut(s) 175, 274, 683, 1015, 1016
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 3 cut(s) 175, 274, 683
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 5 cut(s) 122, 203, 269, 278, 962
NciI CCSGG 2 cut(s) 1015, 1016
NdeII GATC 4 cut(s) 71, 103, 154, 970
NlaIV GGNNCC 2 cut(s) 624, 1231
NmuCI GTSAC 4 cut(s) 560, 656, 1570, 1621
NruI TCGCGA 1 cut(s) 76
NsbI TGCGCA 1 cut(s) 422
NspI RCATGY 7 cut(s) 222, 494, 599, 605, 1028, 1268, 1615
OliI CACNNNNGTG 1 cut(s) 890
PaeI GCATGC 1 cut(s) 494
PagI TCATGA 1 cut(s) 1152
PceI AGGCCT 1 cut(s) 173
PciI ACATGT 2 cut(s) 218, 1611
PciSI GCTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 681
PinAI ACCGGT 1 cut(s) 619
PkrI GCNGC 4 cut(s) 478, 583, 1012, 1202
PmaCI CACGTG 1 cut(s) 1196
PmlI CACGTG 1 cut(s) 1196
Ppu21I YACGTR 2 cut(s) 1006, 1196
PscI ACATGT 2 cut(s) 218, 1611
PshBI ATTAAT 1 cut(s) 1295
PsiI TTATAA 2 cut(s) 168, 245
Psp6I CCWGG 3 cut(s) 173, 272, 681
PspCI CACGTG 1 cut(s) 1196
PspGI CCWGG 3 cut(s) 173, 272, 681
PspN4I GGNNCC 2 cut(s) 624, 1231
PspPI GGNCC 3 cut(s) 177, 331, 1646
PsuI RGATCY 2 cut(s) 103, 970
RruI TCGCGA 1 cut(s) 76
RsaI GTAC 2 cut(s) 203, 642
RsaNI GTAC 2 cut(s) 202, 641
RseI CAYNNNNRTG 4 cut(s) 600, 890, 1029, 1157
SapI GCTCTTC 6 cut(s) 471, 576, 672, 1005, 1245, 1584
SaqAI TTAA 5 cut(s) 1295, 1347, 1451, 1524, 1719
SatI GCNGC 4 cut(s) 477, 582, 1011, 1201
Sau3AI GATC 4 cut(s) 71, 103, 154, 970
Sau96I GGNCC 3 cut(s) 177, 331, 1646
ScrFI CCNGG 5 cut(s) 175, 274, 683, 1015, 1016
SfaNI GCATC 2 cut(s) 745, 943
SfcI CTRYAG 1 cut(s) 1224
SinI GGWCC 3 cut(s) 177, 331, 1646
SmaI CCCGGG 1 cut(s) 1016
SmiMI CAYNNNNRTG 4 cut(s) 600, 890, 1029, 1157
SphI GCATGC 1 cut(s) 494
Sse9I AATT 5 cut(s) 252, 1082, 1145, 1560, 1591
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 2 cut(s) 552, 1092
SspI AATATT 1 cut(s) 847
SspMI CTAG 5 cut(s) 290, 578, 779, 938, 1247
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 5 cut(s) 173, 272, 681, 1013, 1014
StyI CCWWGG 2 cut(s) 192, 480
TaaI ACNGT 4 cut(s) 50, 879, 1182, 1433
TaiI ACGT 3 cut(s) 148, 1008, 1198
TaqI TCGA 6 cut(s) 120, 696, 1185, 1400, 1544, 1641
TasI AATT 5 cut(s) 252, 1082, 1145, 1560, 1591
TatI WGTACW 1 cut(s) 640
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 5 cut(s) 1295, 1347, 1451, 1524, 1719
Tru9I TTAA 5 cut(s) 1295, 1347, 1451, 1524, 1719
TscAI CASTG 4 cut(s) 372, 379, 430, 1000
TseFI GTSAC 4 cut(s) 560, 656, 1570, 1621
TseI GCWGC 4 cut(s) 476, 581, 1010, 1200
Tsp45I GTSAC 4 cut(s) 560, 656, 1570, 1621
TspDTI ATGAA 7 cut(s) 17, 75, 346, 458, 659, 1169, 1572
TspGWI ACGGA 1 cut(s) 1347
TspMI CCCGGG 1 cut(s) 1014
TspRI CASTG 4 cut(s) 372, 379, 430, 1000
VpaK11BI GGWCC 3 cut(s) 177, 331, 1646
VspI ATTAAT 1 cut(s) 1295
XapI RAATTY 2 cut(s) 1145, 1560
XceI RCATGY 7 cut(s) 222, 494, 599, 605, 1028, 1268, 1615
XcmI CCANNNNNNNNNTGG 1 cut(s) 218
XmaI CCCGGG 1 cut(s) 1014
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 5 cut(s) 290, 578, 779, 938, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.