RchiOBHm_Chr7g0243041

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000952
Physical Location & Seq
Forward (+)
69359426 .. 69363532
4107 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21781

Sequence Viewer

Length: 1890 bp
ATGGCAAGTACTCTAGTTGGCTCTAATGCACTTATTCTGGAAATTCTTGACCGTACTAACTATAAATATTGGAGATCCAGATTGAAAGCTTATTTGTTGGCTGAAGATCTCTGGGAGGCTGTCGAAGCAATAGTGGAACCTACAATACTCAAAGATGATCAAGCCGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTGTATGCAATCCAAAATTCATGCGGGAGAGAGTTGTTCCCTTTTATATGTGACATTGAGACGGCAAAAGTTGCCTGGGCGGCTTTGGAACAAGAGTGCAAAGTTGTGCAAGGATATGATGAGAACAATGCCGACCACTTAGTGCGCTTTAAATTGTTCATAGAATCAGTGAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATGCGCAGTGTAGTATTAAGGGCAGTACGTGGACGACATGGAGTAATAGCTCTTCATGTAGCAGCCATGGAAGGGCACGCGCATATCGTAAAAGAGTTGGTGCAGCTGATGACACAAGAAGATTTGAAAACGAAAACCGCTCATGGTCACACAGCTCTTCACGCAGCAGCATGGATGGGGCATGTGCATGTTGTGAAAGAGTTACCGGTGCCGTTGATGGGAGAACTAAAAGACAGTGATGGTGACACAGCTCTTCATATAGCAGTCCAGGAAGGGAAGGTCGATATTGTTAAAGAGTTGGTGTTGCTGATAAGACAAGAAGACTTGAAAATAAAAAACGATGCTGGGTACACGGCTCTTCACCTAGCAGTCAATAAGGGGAATGTGCCTATGGTCAAAGGGTTCATGAGAAAAGAAAAAGATGAGAATAATATTGGCAAGGACGACTTTAAGCATTATATATCGTTTATCACCTATGTGACAAATGGTGATTGGGATCAGGCGAATGAGTATCTTAAAGAACTAGATAATCCTTGTGGTGCAGTAACAGCAGTAGATCCAAGAAACAACTATCGAGATACTGCTCTTCACGTAGCAGCCCTGAAAGGGCATGTGGATGTTACCAAAGGATTGGTATTGTTGCTGAAAATGAGACAAGAAGATTTGGAATTGAAAACCGCTGAAGATTTCACAACTTTTGGTTCTGGTATAACTTTAGGGGTCAGTGAAGAATTCCTCATGGAAAAGGCCAAATATATGGTTGGACAGTACGAGAAAACACTTGGCAGCTGTTTGGAGATACAAAACGCTGAAGGTTTCACAGCTCTTCACCTAGCAGTACTGCGGGGGTATAGGGATATTGTCAAAGAGTTGGTGCCATTAATGAGAAAAGAAGGTTTAGAAATAAGAGACGCTTGTGGACGCACGACTCTTGACATTGCAATCTTACTAAGGCATGTGGATTTTGCGAAGGAGTTAGTGCAATCGATGAGACCAGAGGCTTTGGAAGAAAAAGACGGTCAAGGTTTTACAGCTTTAAATCAGGCTATTCAATGTCTCAATGATGGAGACGTTCAAGAAATGGCTAAATACATGGCTGAAAAGAATGTTAAAGTATTTGGCATATCCTCGGCTCCCCATGATGACATTCCAGTTGTCGACGCTCTTCGTCGGAATAAATGGAAATCGGCTCGCTATATGTATTCTGTCACTCCACTTGAAGCTCTCGAGGGACCCAATGGCAGTTTACTTATTTGCTTATGTCTCAATGCTAGCAACACGATTGATTTGGCATGGGACTTGCTTCAACATTTGCCAAATTTGGCTTTCACTATCACTAAATCGCCTGAATCTACTCCTATGTTGAAATTGGCTCGCATGCATTTTGCATTCTTAAGTGGAACACGACTCACACGTTGGCAGAGATGGCTTTATGATCGAATACATAAGAATTACTCCTCTATGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

629

Amino Acids

70.77

Weight (kDa)

6.12

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 1e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 1.7e-09 gag-polypeptide of LTR copia-type
Ank_2 PF12796 153 - 208 3.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 216 - 280 4.5e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 406 - 468 8.5e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 407 - 454 5.7e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
Acc16I TGCGCA 1 cut(s) 425
AccB1I GGYRCC 2 cut(s) 628, 1294
AccBSI CCGCTC 1 cut(s) 560
AccI GTMKAC 1 cut(s) 1578
AccII CGCG 1 cut(s) 500
AciI CCGC 5 cut(s) 225, 281, 558, 1098, 1264
AclWI GGATC 3 cut(s) 69, 924, 971
AcsI RAATTY 4 cut(s) 42, 217, 1151, 1738
AcuI CTGAAG 3 cut(s) 123, 1122, 1251
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 6 cut(s) 10, 55, 447, 770, 1190, 1260
AfiI CCNNNNNNNGG 3 cut(s) 492, 638, 1026
AflII CTTAAG 1 cut(s) 1813
AflIII ACRYGT 1 cut(s) 1832
AgeI ACCGGT 1 cut(s) 625
AgsI TTSAA 9 cut(s) 85, 547, 748, 1093, 1472, 1496, 1640, 1727, 1786
AjnI CCWGG 3 cut(s) 176, 275, 687
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 9 cut(s) 89, 470, 526, 575, 671, 1209, 1244, 1454, 1643
AluI AGCT 9 cut(s) 89, 470, 526, 575, 671, 1209, 1244, 1454, 1643
Alw26I GTCTC 7 cut(s) 254, 1066, 1323, 1405, 1481, 1482, 1688
AlwI GGATC 3 cut(s) 69, 924, 971
Ama87I CYCGRG 1 cut(s) 1646
AoxI GGCC 2 cut(s) 174, 1167
ApeKI GCWGC 6 cut(s) 482, 523, 584, 587, 1016, 1206
ApoI RAATTY 4 cut(s) 42, 217, 1151, 1738
AseI ATTAAT 1 cut(s) 1301
AsiGI ACCGGT 1 cut(s) 625
AspLEI GCGC 3 cut(s) 348, 426, 502
AspS9I GGNCC 2 cut(s) 180, 1652
AsuHPI GGTGA 6 cut(s) 392, 674, 773, 883, 920, 1241
AsuNHI GCTAGC 1 cut(s) 1691
AvaI CYCGRG 1 cut(s) 1646
AvaII GGWCC 2 cut(s) 180, 1652
BaeGI GKGCMC 1 cut(s) 498
BanI GGYRCC 2 cut(s) 628, 1294
BarI GAAGNNNNNNTAC 2 cut(s) 456, 488
BbsI GAAGAC 1 cut(s) 747
BbvI GCAGC 6 cut(s) 494, 535, 596, 599, 1028, 1218
BccI CCATC 5 cut(s) 589, 631, 653, 1478, 1839
BceAI ACGGC 3 cut(s) 279, 616, 789
BciT130I CCWGG 3 cut(s) 178, 277, 689
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 7 cut(s) 254, 1066, 1323, 1405, 1481, 1482, 1688
BfaI CTAG 5 cut(s) 14, 785, 944, 1253, 1692
BfrI CTTAAG 1 cut(s) 1813
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 7 cut(s) 282, 483, 524, 585, 588, 1017, 1207
BlsI GCNGC 7 cut(s) 283, 484, 525, 586, 589, 1018, 1208
BmcAI AGTACT 2 cut(s) 10, 1260
Bme1390I CCNGG 3 cut(s) 178, 277, 689
Bme18I GGWCC 2 cut(s) 180, 1652
BmeT110I CYCGRG 1 cut(s) 1646
BmgT120I GGNCC 2 cut(s) 180, 1652
BmiI GGNNCC 6 cut(s) 138, 630, 1296, 1554, 1653, 1654
BmrFI CCNGG 3 cut(s) 178, 277, 689
BmsI GCATC 1 cut(s) 751
BmtI GCTAGC 1 cut(s) 1695
BpiI GAAGAC 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 1406
BsaAI YACGTR 2 cut(s) 449, 1012
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 1405
BsaJI CCNNGG 4 cut(s) 195, 276, 486, 1548
BsaWI WCCGGW 1 cut(s) 625
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 638, 1026
Bse118I RCCGGY 1 cut(s) 625
Bse1I ACTGG 1 cut(s) 1571
Bse3DI GCAATG 1 cut(s) 1356
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 3 cut(s) 178, 277, 689
BseCI ATCGAT 1 cut(s) 1406
BseDI CCNNGG 4 cut(s) 195, 276, 486, 1548
BseGI GGATG 2 cut(s) 600, 1042
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 492, 638, 1026
BseMI GCAATG 1 cut(s) 1356
BseNI ACTGG 1 cut(s) 1571
BseRI GAGGAG 1 cut(s) 1867
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 6 cut(s) 494, 535, 596, 599, 1028, 1218
BseYI CCCAGC 1 cut(s) 764
BsgI GTGCAG 2 cut(s) 542, 981
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 2 cut(s) 176, 1169
BshNI GGYRCC 2 cut(s) 628, 1294
BshTI ACCGGT 1 cut(s) 625
BshVI ATCGAT 1 cut(s) 1406
BsiHKCI CYCGRG 1 cut(s) 1646
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 2 cut(s) 1665, 1730
BslI CCNNNNNNNGG 3 cut(s) 492, 638, 1026
BsmAI GTCTC 7 cut(s) 254, 1066, 1323, 1405, 1481, 1482, 1688
BsmBI CGTCTC 3 cut(s) 254, 1323, 1482
BsmFI GGGAC 2 cut(s) 1665, 1730
BsmI GAATGC 2 cut(s) 196, 1808
BsnI GGCC 2 cut(s) 176, 1169
Bso31I GGTCTC 1 cut(s) 1405
BsoBI CYCGRG 1 cut(s) 1646
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 5 cut(s) 225, 281, 558, 1098, 1264
BspANI GGCC 2 cut(s) 176, 1169
BspDI ATCGAT 1 cut(s) 1406
BspFNI CGCG 1 cut(s) 500
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 6 cut(s) 138, 630, 1296, 1554, 1653, 1654
BspOI GCTAGC 1 cut(s) 1695
BspPI GGATC 3 cut(s) 69, 924, 971
BspQI GCTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
BspT107I GGYRCC 2 cut(s) 628, 1294
BspTI CTTAAG 1 cut(s) 1813
BspTNI GGTCTC 1 cut(s) 1405
BsrBI CCGCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 1356
BsrFI RCCGGY 1 cut(s) 625
BsrI ACTGG 1 cut(s) 1571
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 4 cut(s) 195, 276, 486, 1548
BssMI GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
BssT1I CCWWGG 2 cut(s) 195, 486
Bst2UI CCWGG 3 cut(s) 178, 277, 689
Bst4CI ACNGT 4 cut(s) 53, 656, 1188, 1439
Bst6I CTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
BstAFI CTTAAG 1 cut(s) 1813
BstAPI GCANNNNNTGC 1 cut(s) 272
BstBAI YACGTR 2 cut(s) 449, 1012
BstC8I GCNNGC 5 cut(s) 498, 1612, 1693, 1795, 1799
BstDEI CTNAG 2 cut(s) 340, 1370
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 600, 1042
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 3 cut(s) 348, 426, 502
BstKTI GATC 6 cut(s) 77, 109, 160, 919, 979, 1858
BstMAI GTCTC 7 cut(s) 254, 1066, 1323, 1405, 1481, 1482, 1688
BstMBI GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
BstMWI GCNNNNNNNGC 7 cut(s) 125, 206, 272, 281, 581, 968, 1846
BstNI CCWGG 3 cut(s) 178, 277, 689
BstNSI RCATGY 5 cut(s) 605, 611, 1034, 1379, 1801
BstSCI CCNGG 3 cut(s) 176, 275, 687
BstSLI GKGCMC 1 cut(s) 498
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 6 cut(s) 494, 535, 596, 599, 1028, 1218
BstV2I GAAGAC 1 cut(s) 747
BstX2I RGATCY 3 cut(s) 74, 106, 976
BstXI CCANNNNNNTGG 2 cut(s) 1051, 1177
BstYI RGATCY 3 cut(s) 74, 106, 976
Bsu15I ATCGAT 1 cut(s) 1406
BsuRI GGCC 2 cut(s) 176, 1169
BsuTUI ATCGAT 1 cut(s) 1406
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 600, 1042
BtsI GCAGTG 2 cut(s) 382, 433
BtsIMutI CAGTG 5 cut(s) 375, 382, 433, 661, 1150
Cac8I GCNNGC 5 cut(s) 498, 1612, 1693, 1795, 1799
CciI TCATGA 1 cut(s) 825
CfoI GCGC 3 cut(s) 348, 426, 502
Cfr10I RCCGGY 1 cut(s) 625
Cfr13I GGNCC 2 cut(s) 180, 1652
ClaI ATCGAT 1 cut(s) 1406
CseI GACGC 3 cut(s) 1340, 1350, 1589
Csp6I GTAC 6 cut(s) 9, 54, 446, 769, 1189, 1259
CspAI ACCGGT 1 cut(s) 625
CviQI GTAC 6 cut(s) 9, 54, 446, 769, 1189, 1259
DdeI CTNAG 2 cut(s) 340, 1370
DpnI GATC 6 cut(s) 76, 108, 159, 918, 978, 1857
DpnII GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
DraI TTTAAA 2 cut(s) 352, 1458
DraIII CACNNNGTG 1 cut(s) 343
Eam1104I CTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
EarI CTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
Eco130I CCWWGG 2 cut(s) 195, 486
Eco147I AGGCCT 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 1405
Eco47I GGWCC 2 cut(s) 180, 1652
Eco57I CTGAAG 3 cut(s) 123, 1122, 1251
Eco88I CYCGRG 1 cut(s) 1646
EcoO109I RGGNCCY 1 cut(s) 1652
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 3 cut(s) 176, 275, 687
EcoT14I CCWWGG 2 cut(s) 195, 486
EcoT22I ATGCAT 1 cut(s) 1803
ErhI CCWWGG 2 cut(s) 195, 486
Esp3I CGTCTC 3 cut(s) 254, 1323, 1482
FalI AAGNNNNNCTT 4 cut(s) 851, 883, 1318, 1350
FaqI GGGAC 2 cut(s) 1665, 1730
FauI CCCGC 2 cut(s) 218, 1257
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 1578
Fnu4HI GCNGC 7 cut(s) 282, 483, 524, 585, 588, 1017, 1207
FokI GGATG 2 cut(s) 607, 1049
Fsp4HI GCNGC 7 cut(s) 282, 483, 524, 585, 588, 1017, 1207
FspBI CTAG 5 cut(s) 14, 785, 944, 1253, 1692
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 3 cut(s) 347, 425, 501
GluI GCNGC 7 cut(s) 282, 483, 524, 585, 588, 1017, 1207
GsaI CCCAGC 1 cut(s) 768
HaeIII GGCC 2 cut(s) 176, 1169
HapII CCGG 1 cut(s) 626
HgaI GACGC 3 cut(s) 1340, 1350, 1589
HhaI GCGC 3 cut(s) 348, 426, 502
Hin6I GCGC 3 cut(s) 346, 424, 500
HinP1I GCGC 3 cut(s) 346, 424, 500
HincII GTYRAC 1 cut(s) 1579
HindII GTYRAC 1 cut(s) 1579
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 4 cut(s) 365, 1348, 1769, 1827
HpaII CCGG 1 cut(s) 626
HphI GGTGA 6 cut(s) 392, 674, 773, 883, 920, 1241
Hpy166II GTNNAC 5 cut(s) 452, 771, 1340, 1579, 1667
Hpy188I TCNGA 1 cut(s) 1593
Hpy188III TCNNGA 8 cut(s) 38, 47, 78, 826, 995, 1352, 1496, 1646
Hpy8I GTNNAC 5 cut(s) 452, 771, 1340, 1579, 1667
Hpy99I CGWCG 2 cut(s) 1583, 1593
HpyAV CCTTC 6 cut(s) 485, 686, 691, 1226, 1307, 1384
HpyCH4III ACNGT 4 cut(s) 53, 656, 1188, 1439
HpyCH4IV ACGT 4 cut(s) 448, 1011, 1491, 1834
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 206, 272, 281, 581, 968, 1846
HpyF3I CTNAG 2 cut(s) 340, 1370
HpySE526I ACGT 4 cut(s) 448, 1011, 1491, 1834
HspAI GCGC 3 cut(s) 346, 424, 500
KflI GGGWCCC 1 cut(s) 1652
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
LguI GCTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
LmnI GCTCC 1 cut(s) 1558
Lsp1109I GCAGC 6 cut(s) 494, 535, 596, 599, 1028, 1218
LweI GCATC 1 cut(s) 751
MaeI CTAG 5 cut(s) 14, 785, 944, 1253, 1692
MaeII ACGT 4 cut(s) 448, 1011, 1491, 1834
MaeIII GTNAC 8 cut(s) 251, 566, 621, 662, 898, 964, 1039, 1627
MalI GATC 6 cut(s) 76, 108, 159, 918, 978, 1857
MbiI CCGCTC 1 cut(s) 560
MboI GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
MflI RGATCY 3 cut(s) 74, 106, 976
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 8 cut(s) 42, 217, 353, 1088, 1151, 1738, 1787, 1870
MlyI GAGTC 2 cut(s) 1342, 1821
MmeI TCCRAC 2 cut(s) 1162, 1571
MnlI CCTC 6 cut(s) 109, 1166, 1411, 1558, 1642, 1888
Mph1103I ATGCAT 1 cut(s) 1803
MslI CAYNNNNRTG 3 cut(s) 606, 896, 1035
MspA1I CMGCKG 3 cut(s) 526, 1100, 1209
MspCI CTTAAG 1 cut(s) 1813
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 3 cut(s) 178, 277, 689
Mva1269I GAATGC 2 cut(s) 196, 1808
MvaI CCWGG 3 cut(s) 178, 277, 689
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 7 cut(s) 125, 206, 272, 281, 581, 968, 1846
NcoI CCATGG 1 cut(s) 486
NdeII GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
NheI GCTAGC 1 cut(s) 1691
NlaIV GGNNCC 6 cut(s) 138, 630, 1296, 1554, 1653, 1654
NmeAIII GCCGAG 1 cut(s) 1529
NmuCI GTSAC 5 cut(s) 251, 566, 662, 898, 1627
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 1803
NspI RCATGY 5 cut(s) 605, 611, 1034, 1379, 1801
OliI CACNNNNGTG 1 cut(s) 896
PaeI GCATGC 1 cut(s) 1801
PaeR7I CTCGAG 1 cut(s) 1646
PagI TCATGA 1 cut(s) 825
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 1831
PctI GAATGC 2 cut(s) 196, 1808
PfeI GAWTC 2 cut(s) 365, 1769
PfoI TCCNGGA 1 cut(s) 687
PinAI ACCGGT 1 cut(s) 625
PkrI GCNGC 7 cut(s) 283, 484, 525, 586, 589, 1018, 1208
PleI GAGTC 2 cut(s) 1342, 1821
PpsI GAGTC 2 cut(s) 1342, 1821
Ppu21I YACGTR 2 cut(s) 449, 1012
PpuMI RGGWCCY 1 cut(s) 1652
PshBI ATTAAT 1 cut(s) 1301
PsiI TTATAA 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 1652
Psp6I CCWGG 3 cut(s) 176, 275, 687
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 3 cut(s) 176, 275, 687
PspN4I GGNNCC 6 cut(s) 138, 630, 1296, 1554, 1653, 1654
PspPI GGNCC 2 cut(s) 180, 1652
PspPPI RGGWCCY 1 cut(s) 1652
PsrI GAACNNNNNNTAC 2 cut(s) 129, 161
PsuI RGATCY 3 cut(s) 74, 106, 976
PvuII CAGCTG 2 cut(s) 526, 1209
RsaI GTAC 6 cut(s) 10, 55, 447, 770, 1190, 1260
RsaNI GTAC 6 cut(s) 9, 54, 446, 769, 1189, 1259
RseI CAYNNNNRTG 3 cut(s) 606, 896, 1035
SalI GTCGAC 1 cut(s) 1577
SapI GCTCTTC 7 cut(s) 477, 582, 678, 783, 1011, 1251, 1590
SatI GCNGC 7 cut(s) 282, 483, 524, 585, 588, 1017, 1207
Sau3AI GATC 6 cut(s) 74, 106, 157, 916, 976, 1855
Sau96I GGNCC 2 cut(s) 180, 1652
ScaI AGTACT 2 cut(s) 10, 1260
SchI GAGTC 2 cut(s) 1342, 1821
ScrFI CCNGG 3 cut(s) 178, 277, 689
SduI GDGCHC 1 cut(s) 498
SfaNI GCATC 1 cut(s) 751
Sfr274I CTCGAG 1 cut(s) 1646
SinI GGWCC 2 cut(s) 180, 1652
SlaI CTCGAG 1 cut(s) 1646
SmiMI CAYNNNNRTG 3 cut(s) 606, 896, 1035
SmlI CTYRAG 2 cut(s) 1646, 1813
SmoI CTYRAG 2 cut(s) 1646, 1813
SphI GCATGC 1 cut(s) 1801
Sse9I AATT 8 cut(s) 42, 217, 353, 1088, 1151, 1738, 1787, 1870
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 5 cut(s) 225, 281, 558, 1098, 1264
SspI AATATT 2 cut(s) 68, 853
SspMI CTAG 5 cut(s) 14, 785, 944, 1253, 1692
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 176, 275, 687
StyI CCWWGG 2 cut(s) 195, 486
TaaI ACNGT 4 cut(s) 53, 656, 1188, 1439
TaiI ACGT 4 cut(s) 451, 1014, 1494, 1837
TaqI TCGA 7 cut(s) 123, 702, 994, 1406, 1578, 1647, 1858
TasI AATT 8 cut(s) 42, 217, 353, 1088, 1151, 1738, 1787, 1870
TatI WGTACW 2 cut(s) 8, 1258
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 2 cut(s) 365, 1769
TscAI CASTG 5 cut(s) 375, 382, 433, 661, 1150
TseFI GTSAC 5 cut(s) 251, 566, 662, 898, 1627
TseI GCWGC 6 cut(s) 482, 523, 584, 587, 1016, 1206
Tsp45I GTSAC 5 cut(s) 251, 566, 662, 898, 1627
TspDTI ATGAA 5 cut(s) 210, 349, 464, 665, 814
TspRI CASTG 5 cut(s) 375, 382, 433, 661, 1150
Vha464I CTTAAG 1 cut(s) 1813
VpaK11BI GGWCC 2 cut(s) 180, 1652
VspI ATTAAT 1 cut(s) 1301
XapI RAATTY 4 cut(s) 42, 217, 1151, 1738
XceI RCATGY 5 cut(s) 605, 611, 1034, 1379, 1801
XhoI CTCGAG 1 cut(s) 1646
XmiI GTMKAC 1 cut(s) 1578
XspI CTAG 5 cut(s) 14, 785, 944, 1253, 1692
ZrmI AGTACT 2 cut(s) 10, 1260
Zsp2I ATGCAT 1 cut(s) 1803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.