Rroxscaffold_4G00325490

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000952
Physical Location & Seq
Forward (+)
57220791 .. 57227584
6794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325490.1

Sequence Viewer

Length: 333 bp
ATGCTTACCAATATTTTAGGTGTACAAAATGCAAAAGGTTTCACAGCCCTTCATCTAGCAGTATTGATGCGGAATGTAGATGTTGTGAAAGAGTTGGTGCCATTCATGGGAGAAGAAGGTTTAGAAATAAAGGGCGCTGATGGAGACACAGCTCTTCACATCTCGGTCTTACAGGGGCAGCTACACTCAATTTCCTTGACTATGGACAATGAATTATTCTTGAACCATGGCTTGTTAGAGAGGTTTCAGGTTTTTGTTCTCTTTGAAGGACCGGTTACATCCATAATAAAATCTCATGTATCTAGTAAGAGGATGAATTATATCTATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

110

Amino Acids

12.2

Weight (kDa)

5.89

Isoelectric Point (pI)

25.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 64 1.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AciI CCGC 1 cut(s) 70
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 271
AgsI TTSAA 2 cut(s) 223, 266
AluBI AGCT 2 cut(s) 152, 181
AluI AGCT 2 cut(s) 152, 181
Alw26I GTCTC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 178
AsiGI ACCGGT 1 cut(s) 271
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 269
AvaII GGWCC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 190
BccI CCATC 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 2 cut(s) 56, 303
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 99
BmsI GCATC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 2 cut(s) 278, 318
BseLI CCNNNNNNNGG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 190
BshNI GGYRCC 1 cut(s) 97
BshTI ACCGGT 1 cut(s) 271
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 271
BsrGI TGTACA 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 2 cut(s) 278, 318
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 278, 318
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 23
CspAI ACCGGT 1 cut(s) 271
CviAII CATG 3 cut(s) 106, 227, 296
CviJI RGCY 4 cut(s) 47, 152, 181, 231
CviKI_1 RGCY 4 cut(s) 47, 152, 181, 231
CviQI GTAC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 3 cut(s) 109, 230, 299
FaiI YATR 7 cut(s) 107, 203, 228, 284, 297, 321, 331
FatI CATG 3 cut(s) 105, 226, 295
Fnu4HI GCNGC 1 cut(s) 179
FokI GGATG 2 cut(s) 265, 325
Fsp4HI GCNGC 1 cut(s) 179
FspBI CTAG 2 cut(s) 56, 303
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 138
HapII CCGG 1 cut(s) 272
HhaI GCGC 1 cut(s) 137
Hin1II CATG 3 cut(s) 109, 230, 299
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HpaII CCGG 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 23
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 59, 110, 260
HpyCH4V TGCA 1 cut(s) 32
Hsp92II CATG 3 cut(s) 109, 230, 299
HspAI GCGC 1 cut(s) 135
LguI GCTCTTC 1 cut(s) 159
LpnPI CCDG 3 cut(s) 158, 233, 285
Lsp1109I GCAGC 1 cut(s) 190
LweI GCATC 1 cut(s) 57
MaeI CTAG 2 cut(s) 56, 303
MaeIII GTNAC 1 cut(s) 274
MboII GAAGA 2 cut(s) 125, 146
MluCI AATT 3 cut(s) 189, 212, 316
MnlI CCTC 2 cut(s) 234, 303
MspI CCGG 1 cut(s) 272
NcoI CCATGG 1 cut(s) 226
NlaIII CATG 3 cut(s) 109, 230, 299
NlaIV GGNNCC 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 271
PkrI GCNGC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 269
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 1 cut(s) 179
Sau96I GGNCC 1 cut(s) 269
SetI ASST 7 cut(s) 22, 40, 121, 154, 183, 245, 252
SfaNI GCATC 1 cut(s) 57
SinI GGWCC 1 cut(s) 269
Sse9I AATT 3 cut(s) 189, 212, 316
SsiI CCGC 1 cut(s) 70
SspI AATATT 1 cut(s) 13
SspMI CTAG 2 cut(s) 56, 303
StyI CCWWGG 1 cut(s) 226
TaqII GACCGA 1 cut(s) 154
TasI AATT 3 cut(s) 189, 212, 316
TatI WGTACW 1 cut(s) 22
TseI GCWGC 1 cut(s) 178
TspDTI ATGAA 5 cut(s) 41, 94, 225, 318, 329
VpaK11BI GGWCC 1 cut(s) 269
XspI CTAG 2 cut(s) 56, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.