Rroxscaffold_4G00316280

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000952
Physical Location & Seq
Reverse (-)
41024977 .. 41042264
17288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316280.1

Sequence Viewer

Length: 1602 bp
ATGATTCCTTCTAACCTTCCATATCCTTTCCACCGAAAGGACAACATAAGTCACCGCTGTGACAATGTTCTATTTGCTAACTTAACCATCAAGAAGCAATTCAACGTTCACCACGGCTCGAATCGTGAAGAATCACTTCCACGAAGCGTAGAGGAGGTTGAGGCGAAGAACGAGGAGGAAGAACGAAGCAGGCGAAGATATGATGAGAACAATCCCGACCACTCGATCCGCTATAAATCATTTATCGAATCTGTGAGGAGTGGTGATTGGGACGAGGCAAAGAAGTGTCTTAAAGAAGTTGATGTTCGCAGTGTAGTAGTGAGAGCAATAGACGAATGGGAAGGAGAAATAGCTCTTCATGTAGCAACCAAGGAAGGACACGTGCATATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAGGACTTGAAAACGAAAACCGCTCATGGTCACACAGCTCTTCACGTAGCAGCATGGATGGGGCATGTGCAAATCGTGAAAGAGTTACCGGTGCCGTTGATGGGAGAAGTACAAGACTGGGAAGGTGACACAGCTCTTCATATAGCAGTCCAGGAAGGGAAGGTCAATATTGTTAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACCTAGAAATAAAAAATGCTGCTGGTTACACAGCTCTTCACCTAGCAGTCAATGAGGGGAATGTGCCTATGGTCAAAGAGTTCGTGAGAAAAGAAAAAGGTGAGAATAATATTGGCGAGAACGACTTTGAGCGTTTTATACCGTTTACCACCTACGTGCAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCTTGTGGTGCAGTAACAGCAGTAGATCCAAGAGATGACTACCGAGAAACTGCTCTTCACGTAGCAGCCCGGCAAGGGCATTTGGACGTTACCAGAGGATTGGTGTTGTTGCTGGAAATAAGACAAGACGATTTGGAATTGAAAACCGCTGAAAATTTCACAAATTTTGGTTATGGTATAACTTTAGGGGTCAATGAAGAATTCCCCATGGAAAGGGCCAAATACATGGTCGAACAGTATGAAAAAACACTTGGCAGTGTTTTGGAGGTACAAAACGCTGACGGGTCCACACCTCTTCACCTAGCAGTATTGCTAGGGTATGGGGATATTGTGAATGAATTGGTGCCATTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATCTTACAAGGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACCAGAGGGAATAACCGCTACACCATGGAATAATGCACCAGGATTGCAAACAACAAACCCGTCGACTCGGAAAAGCTCCCGTGACCACGAAGCTCTCATCTTTAGGCCCAACTACATAGCTCGAACCCTAGTTGATGGCATTTTCCATGTTAAAGGAAGTATGGATCTTCAATCTATTTTGGGATATTCGACTCACTACAATCTGAAGTTTGTTAAGTTTTGGGAACCCACATTTCCAATCCGGTGGGGCTTAGTTAAACTTAGGCTTGATGGGTATTATTCGAATCATCAAAGAAGTCGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

533

Amino Acids

60.69

Weight (kDa)

5.62

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 110 - 169 5.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 177 - 224 2.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 179 - 240 9.9e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 366 - 429 1.1e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 511, 1177
AccBSI CCGCTC 1 cut(s) 443
AccI GTMKAC 1 cut(s) 1358
AciI CCGC 5 cut(s) 55, 229, 441, 981, 1311
AclI AACGTT 1 cut(s) 105
AclWI GGATC 3 cut(s) 220, 854, 1467
AcsI RAATTY 3 cut(s) 988, 997, 1034
AcuI CTGAAG 1 cut(s) 1520
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 2 cut(s) 531, 1104
AfiI CCNNNNNNNGG 4 cut(s) 37, 521, 909, 1047
AflIII ACRYGT 1 cut(s) 379
AgeI ACCGGT 1 cut(s) 508
AgsI TTSAA 4 cut(s) 103, 430, 976, 1466
AjnI CCWGG 2 cut(s) 570, 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 1068, 1100
AleI CACNNNNGTG 2 cut(s) 57, 779
AluBI AGCT 8 cut(s) 353, 458, 554, 659, 1232, 1371, 1388, 1415
AluI AGCT 8 cut(s) 353, 458, 554, 659, 1232, 1371, 1388, 1415
Alw26I GTCTC 3 cut(s) 610, 1218, 1288
AlwI GGATC 3 cut(s) 220, 854, 1467
AoxI GGCC 2 cut(s) 1050, 1400
ApeKI GCWGC 3 cut(s) 470, 644, 899
ApoI RAATTY 3 cut(s) 988, 997, 1034
ArsI GACNNNNNNTTYG 2 cut(s) 1047, 1079
AseI ATTAAT 1 cut(s) 1184
AsiGI ACCGGT 1 cut(s) 508
Asp700I GAANNNNTTC 2 cut(s) 98, 135
AspS9I GGNCC 3 cut(s) 1050, 1119, 1401
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 8 cut(s) 44, 101, 275, 557, 656, 737, 803, 1124
AsuII TTCGAA 1 cut(s) 1577
AvaII GGWCC 1 cut(s) 1119
BanI GGYRCC 2 cut(s) 511, 1177
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 3 cut(s) 482, 631, 911
BccI CCATC 6 cut(s) 95, 472, 514, 1214, 1424, 1559
BceAI ACGGC 2 cut(s) 130, 499
BciT130I CCWGG 2 cut(s) 572, 1335
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 610, 1218, 1288
BfaI CTAG 6 cut(s) 629, 668, 827, 1136, 1148, 1424
BisI GCNGC 3 cut(s) 471, 645, 900
BlsI GCNGC 3 cut(s) 472, 646, 901
Bme1390I CCNGG 3 cut(s) 572, 904, 1335
Bme18I GGWCC 1 cut(s) 1119
BmgT120I GGNCC 3 cut(s) 1050, 1119, 1401
BmiI GGNNCC 4 cut(s) 513, 1120, 1179, 1521
BmrFI CCNGG 3 cut(s) 572, 904, 1335
BmrI ACTGGG 1 cut(s) 547
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 1577
BpuMI CCSGG 1 cut(s) 904
Bsa29I ATCGAT 1 cut(s) 1289
BsaAI YACGTR 4 cut(s) 382, 466, 781, 895
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 4 cut(s) 112, 369, 1041, 1319
BsaWI WCCGGW 2 cut(s) 508, 1536
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 521, 909, 1047
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 542
Bse3DI GCAATG 1 cut(s) 1239
BseBI CCWGG 2 cut(s) 572, 1335
BseCI ATCGAT 1 cut(s) 1289
BseDI CCNNGG 4 cut(s) 112, 369, 1041, 1319
BseGI GGATG 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 37, 521, 909, 1047
BseMI GCAATG 1 cut(s) 1239
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 3 cut(s) 167, 188, 271
BseXI GCAGC 3 cut(s) 482, 631, 911
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 2 cut(s) 1052, 1402
BshNI GGYRCC 2 cut(s) 511, 1177
BshTI ACCGGT 1 cut(s) 508
BshVI ATCGAT 1 cut(s) 1289
BsiSI CCGG 3 cut(s) 509, 904, 1537
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 4 cut(s) 37, 521, 909, 1047
BsmAI GTCTC 3 cut(s) 610, 1218, 1288
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 2 cut(s) 1052, 1402
Bso31I GGTCTC 1 cut(s) 1288
Bsp119I TTCGAA 1 cut(s) 1577
Bsp143I GATC 3 cut(s) 225, 859, 1459
Bsp19I CCATGG 2 cut(s) 1041, 1319
BspACI CCGC 5 cut(s) 55, 229, 441, 981, 1311
BspANI GGCC 2 cut(s) 1052, 1402
BspDI ATCGAT 1 cut(s) 1289
BspLI GGNNCC 4 cut(s) 513, 1120, 1179, 1521
BspPI GGATC 3 cut(s) 220, 854, 1467
BspQI GCTCTTC 5 cut(s) 360, 465, 561, 666, 894
BspT104I TTCGAA 1 cut(s) 1577
BspT107I GGYRCC 2 cut(s) 511, 1177
BspTNI GGTCTC 1 cut(s) 1288
BsrBI CCGCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 1239
BsrFI RCCGGY 1 cut(s) 508
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 4 cut(s) 112, 369, 1041, 1319
BssMI GATC 3 cut(s) 225, 859, 1459
BssT1I CCWWGG 3 cut(s) 369, 1041, 1319
Bst2UI CCWGG 2 cut(s) 572, 1335
Bst4CI ACNGT 2 cut(s) 768, 1071
Bst6I CTCTTC 6 cut(s) 360, 465, 561, 666, 894, 1134
BstBAI YACGTR 4 cut(s) 382, 466, 781, 895
BstBI TTCGAA 1 cut(s) 1577
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 3 cut(s) 818, 1546, 1556
BstDSI CCRYGG 3 cut(s) 112, 1041, 1319
BstF5I GGATG 1 cut(s) 483
BstKTI GATC 3 cut(s) 228, 862, 1462
BstMAI GTCTC 3 cut(s) 610, 1218, 1288
BstMBI GATC 3 cut(s) 225, 859, 1459
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 2 cut(s) 572, 1335
BstNSI RCATGY 2 cut(s) 488, 1262
BstSCI CCNGG 3 cut(s) 570, 902, 1333
BstV1I GCAGC 3 cut(s) 482, 631, 911
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 2 cut(s) 859, 1459
BstXI CCANNNNNNTGG 3 cut(s) 934, 1060, 1539
BstYI RGATCY 2 cut(s) 859, 1459
Bsu15I ATCGAT 1 cut(s) 1289
BsuRI GGCC 2 cut(s) 1052, 1402
BsuTUI ATCGAT 1 cut(s) 1289
BtgI CCRYGG 3 cut(s) 112, 1041, 1319
BtsCI GGATG 1 cut(s) 483
BtsI GCAGTG 2 cut(s) 316, 1096
BtsIMutI CAGTG 2 cut(s) 316, 1096
Cac8I GCNNGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 3 cut(s) 1050, 1119, 1401
ClaI ATCGAT 1 cut(s) 1289
CseI GACGC 1 cut(s) 1223
Csp6I GTAC 2 cut(s) 530, 1103
CspAI ACCGGT 1 cut(s) 508
CviAII CATG 9 cut(s) 359, 446, 474, 485, 1042, 1060, 1259, 1320, 1442
CviQI GTAC 2 cut(s) 530, 1103
DdeI CTNAG 3 cut(s) 818, 1546, 1556
DpnI GATC 3 cut(s) 227, 861, 1461
DpnII GATC 3 cut(s) 225, 859, 1459
Eam1104I CTCTTC 6 cut(s) 360, 465, 561, 666, 894, 1134
EarI CTCTTC 6 cut(s) 360, 465, 561, 666, 894, 1134
Eco130I CCWWGG 3 cut(s) 369, 1041, 1319
Eco31I GGTCTC 1 cut(s) 1288
Eco47I GGWCC 1 cut(s) 1119
Eco57I CTGAAG 1 cut(s) 1520
Eco72I CACGTG 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 1034
EcoRII CCWGG 2 cut(s) 570, 1333
EcoT14I CCWWGG 3 cut(s) 369, 1041, 1319
ErhI CCWWGG 3 cut(s) 369, 1041, 1319
FaeI CATG 9 cut(s) 362, 449, 477, 488, 1045, 1063, 1262, 1323, 1445
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 1 cut(s) 284
FatI CATG 9 cut(s) 358, 445, 473, 484, 1041, 1059, 1258, 1319, 1441
FblI GTMKAC 1 cut(s) 1358
Fnu4HI GCNGC 3 cut(s) 471, 645, 900
FokI GGATG 1 cut(s) 490
Fsp4HI GCNGC 3 cut(s) 471, 645, 900
FspBI CTAG 6 cut(s) 629, 668, 827, 1136, 1148, 1424
GluI GCNGC 3 cut(s) 471, 645, 900
HaeIII GGCC 2 cut(s) 1052, 1402
HapII CCGG 3 cut(s) 509, 904, 1537
HgaI GACGC 1 cut(s) 1223
Hin1II CATG 9 cut(s) 362, 449, 477, 488, 1045, 1063, 1262, 1323, 1445
HincII GTYRAC 1 cut(s) 1359
HindII GTYRAC 1 cut(s) 1359
HinfI GANTC 7 cut(s) 4, 121, 131, 248, 1360, 1486, 1579
HpaII CCGG 3 cut(s) 509, 904, 1537
HphI GGTGA 8 cut(s) 44, 101, 275, 557, 656, 737, 803, 1124
Hpy166II GTNNAC 4 cut(s) 109, 771, 1122, 1359
Hpy188I TCNGA 2 cut(s) 1365, 1500
Hpy188III TCNNGA 6 cut(s) 91, 125, 215, 391, 496, 709
Hpy8I GTNNAC 4 cut(s) 109, 771, 1122, 1359
Hpy99I CGWCG 1 cut(s) 1360
HpyAV CCTTC 9 cut(s) 18, 26, 335, 368, 536, 569, 574, 1190, 1267
HpyCH4III ACNGT 2 cut(s) 768, 1071
HpyCH4IV ACGT 6 cut(s) 105, 381, 465, 780, 894, 921
HpyCH4V TGCA 9 cut(s) 385, 406, 490, 784, 845, 1244, 1285, 1331, 1342
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 3 cut(s) 818, 1546, 1556
HpySE526I ACGT 6 cut(s) 105, 381, 465, 780, 894, 921
Hsp92II CATG 9 cut(s) 362, 449, 477, 488, 1045, 1063, 1262, 1323, 1445
Kzo9I GATC 3 cut(s) 225, 859, 1459
LguI GCTCTTC 5 cut(s) 360, 465, 561, 666, 894
LmnI GCTCC 1 cut(s) 1376
Lsp1109I GCAGC 3 cut(s) 482, 631, 911
MaeI CTAG 6 cut(s) 629, 668, 827, 1136, 1148, 1424
MaeII ACGT 6 cut(s) 105, 381, 465, 780, 894, 921
MaeIII GTNAC 9 cut(s) 50, 59, 449, 504, 545, 650, 847, 922, 1376
MalI GATC 3 cut(s) 227, 861, 1461
MbiI CCGCTC 1 cut(s) 443
MboI GATC 3 cut(s) 225, 859, 1459
MflI RGATCY 2 cut(s) 859, 1459
MluCI AATT 6 cut(s) 98, 971, 988, 997, 1034, 1172
MlyI GAGTC 2 cut(s) 1354, 1480
MroXI GAANNNNTTC 2 cut(s) 98, 135
MseI TTAA 8 cut(s) 83, 291, 594, 1184, 1236, 1446, 1509, 1551
MslI CAYNNNNRTG 2 cut(s) 57, 779
MspA1I CMGCKG 2 cut(s) 57, 983
MspI CCGG 3 cut(s) 509, 904, 1537
MspR9I CCNGG 3 cut(s) 572, 904, 1335
MvaI CCWGG 2 cut(s) 572, 1335
MwoI GCNNNNNNNGC 1 cut(s) 851
NciI CCSGG 1 cut(s) 904
NcoI CCATGG 2 cut(s) 1041, 1319
NdeII GATC 3 cut(s) 225, 859, 1459
NlaIII CATG 9 cut(s) 362, 449, 477, 488, 1045, 1063, 1262, 1323, 1445
NlaIV GGNNCC 4 cut(s) 513, 1120, 1179, 1521
NmuCI GTSAC 5 cut(s) 50, 59, 449, 545, 1376
NspI RCATGY 2 cut(s) 488, 1262
NspV TTCGAA 1 cut(s) 1577
OliI CACNNNNGTG 2 cut(s) 57, 779
PciSI GCTCTTC 5 cut(s) 360, 465, 561, 666, 894
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 387
PdmI GAANNNNTTC 2 cut(s) 98, 135
PfeI GAWTC 5 cut(s) 4, 121, 131, 248, 1579
PfoI TCCNGGA 1 cut(s) 570
PinAI ACCGGT 1 cut(s) 508
PkrI GCNGC 3 cut(s) 472, 646, 901
PleI GAGTC 2 cut(s) 1354, 1480
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 2 cut(s) 1354, 1480
Ppu21I YACGTR 4 cut(s) 382, 466, 781, 895
PshBI ATTAAT 1 cut(s) 1184
Psp1406I AACGTT 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 570, 1333
PspCI CACGTG 1 cut(s) 382
PspGI CCWGG 2 cut(s) 570, 1333
PspN4I GGNNCC 4 cut(s) 513, 1120, 1179, 1521
PspPI GGNCC 3 cut(s) 1050, 1119, 1401
PsuI RGATCY 2 cut(s) 859, 1459
RsaI GTAC 2 cut(s) 531, 1104
RsaNI GTAC 2 cut(s) 530, 1103
RseI CAYNNNNRTG 2 cut(s) 57, 779
SalI GTCGAC 1 cut(s) 1357
SapI GCTCTTC 5 cut(s) 360, 465, 561, 666, 894
SaqAI TTAA 8 cut(s) 83, 291, 594, 1184, 1236, 1446, 1509, 1551
SatI GCNGC 3 cut(s) 471, 645, 900
Sau3AI GATC 3 cut(s) 225, 859, 1459
Sau96I GGNCC 3 cut(s) 1050, 1119, 1401
SchI GAGTC 2 cut(s) 1354, 1480
ScrFI CCNGG 3 cut(s) 572, 904, 1335
SfuI TTCGAA 1 cut(s) 1577
SinI GGWCC 1 cut(s) 1119
SmiMI CAYNNNNRTG 2 cut(s) 57, 779
Sse9I AATT 6 cut(s) 98, 971, 988, 997, 1034, 1172
SsiI CCGC 5 cut(s) 55, 229, 441, 981, 1311
SspI AATATT 2 cut(s) 589, 736
SspMI CTAG 6 cut(s) 629, 668, 827, 1136, 1148, 1424
StyD4I CCNGG 3 cut(s) 570, 902, 1333
StyI CCWWGG 3 cut(s) 369, 1041, 1319
TaaI ACNGT 2 cut(s) 768, 1071
TaiI ACGT 6 cut(s) 108, 384, 468, 783, 897, 924
TaqI TCGA 9 cut(s) 119, 224, 246, 1065, 1289, 1358, 1417, 1484, 1577
TasI AATT 6 cut(s) 98, 971, 988, 997, 1034, 1172
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 5 cut(s) 4, 121, 131, 248, 1579
Tru1I TTAA 8 cut(s) 83, 291, 594, 1184, 1236, 1446, 1509, 1551
Tru9I TTAA 8 cut(s) 83, 291, 594, 1184, 1236, 1446, 1509, 1551
TscAI CASTG 2 cut(s) 316, 1096
TseFI GTSAC 5 cut(s) 50, 59, 449, 545, 1376
TseI GCWGC 3 cut(s) 470, 644, 899
Tsp45I GTSAC 5 cut(s) 50, 59, 449, 545, 1376
TspDTI ATGAA 5 cut(s) 347, 548, 1044, 1089, 1185
TspRI CASTG 2 cut(s) 316, 1096
VpaK11BI GGWCC 1 cut(s) 1119
VspI ATTAAT 1 cut(s) 1184
XapI RAATTY 3 cut(s) 988, 997, 1034
XceI RCATGY 2 cut(s) 488, 1262
XmiI GTMKAC 1 cut(s) 1358
XmnI GAANNNNTTC 2 cut(s) 98, 135
XspI CTAG 6 cut(s) 629, 668, 827, 1136, 1148, 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.