Rh7AG474100

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000952
Physical Location & Seq
Forward (+)
65746287 .. 65746923
637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG474100.1

Sequence Viewer

Length: 637 bp
ATGAGAGAAGAAGATTTGGAATTGAAAACTGCTGAAGATTTCACAACTTTTGGGTTTGGTATAACTTTAGGGGTCGATGAAGAATTCCTCATGGAAAGGGCCAAATACATGGTTGAACAGTATGAGAAAACATTAAGCAGTGTTTTGGAGATACAAAACGCTGAAGGGTCCACAGCTCTTCACCTAGCAGCACTGCTAGGGTACAGGGATATTGGGAAAGAGTTGGTGCCATTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATCGCAATCTTACAAGGGCTTGAAGATATTGCGAAGGAGTCGGTGCAATCGATGAGACCAGAGGGTGTGGAAATAAAAGACGGTCGGGGTTTTACAGCTTTAAATTGGGCTGTCCAACGTTTCAAGGATGGAGACATTCACGAAATTGCTAAATACATGGCTGAAAAGAATGGTAAAGTATTTGGCATACCCTCCGCTCCCGATGATGACATTCCAGTTGTCACAGCTCTTCGTTGGAGCAAATGGGATTCGGCTCGCTATATCTATTCGGTCACCCCGCATACAGCTCTCGAGGGAGCCAATGGCAGTTCACTTATTTGCGCATGTCTCAATGCTAGCAAAACGATTG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

212

Amino Acids

23.42

Weight (kDa)

4.71

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 50 - 108 2.9e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 610
AccB1I GGYRCC 1 cut(s) 226
AccBSI CCGCTC 1 cut(s) 485
AciI CCGC 2 cut(s) 483, 566
AclI AACGTT 1 cut(s) 406
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 2 cut(s) 54, 183
AfaI GTAC 1 cut(s) 203
AgsI TTSAA 4 cut(s) 25, 116, 311, 412
AluBI AGCT 5 cut(s) 176, 281, 386, 515, 575
AluI AGCT 5 cut(s) 176, 281, 386, 515, 575
Alw26I GTCTC 4 cut(s) 267, 337, 414, 620
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 83
AseI ATTAAT 1 cut(s) 233
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 2 cut(s) 99, 168
AsuHPI GGTGA 2 cut(s) 173, 553
AsuNHI GCTAGC 1 cut(s) 623
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 200
BccI CCATC 2 cut(s) 263, 410
BcoDI GTCTC 4 cut(s) 267, 337, 414, 620
BfaI CTAG 3 cut(s) 185, 197, 624
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 99, 168
BmiI GGNNCC 3 cut(s) 169, 228, 586
BmtI GCTAGC 1 cut(s) 627
Bsa29I ATCGAT 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 503
BseCI ATCGAT 1 cut(s) 338
BseGI GGATG 1 cut(s) 421
BseNI ACTGG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 578
BsmAI GTCTC 4 cut(s) 267, 337, 414, 620
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 578
BspACI CCGC 2 cut(s) 483, 566
BspANI GGCC 1 cut(s) 101
BspDI ATCGAT 1 cut(s) 338
BspLI GGNNCC 3 cut(s) 169, 228, 586
BspOI GCTAGC 1 cut(s) 627
BspQI GCTCTTC 2 cut(s) 183, 522
BspT107I GGYRCC 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 337
BsrBI CCGCTC 1 cut(s) 485
BsrI ACTGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 120, 371
Bst6I CTCTTC 2 cut(s) 183, 522
BstC8I GCNNGC 2 cut(s) 544, 625
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 1 cut(s) 421
BstHHI GCGC 1 cut(s) 611
BstMAI GTCTC 4 cut(s) 267, 337, 414, 620
BstMCI CGRYCG 1 cut(s) 373
BstNSI RCATGY 1 cut(s) 615
BstPI GGTNACC 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 200
BstXI CCANNNNNNTGG 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 338
BsuRI GGCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 2 cut(s) 145, 191
BtsIMutI CAGTG 2 cut(s) 145, 191
Cac8I GCNNGC 2 cut(s) 544, 625
CfoI GCGC 1 cut(s) 611
Cfr13I GGNCC 2 cut(s) 99, 168
ClaI ATCGAT 1 cut(s) 338
CseI GACGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 202
CviAII CATG 4 cut(s) 91, 109, 445, 612
CviQI GTAC 1 cut(s) 202
DraI TTTAAA 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 183, 522
EarI CTCTTC 2 cut(s) 183, 522
Eco31I GGTCTC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 54, 183
Eco88I CYCGRG 1 cut(s) 578
Eco91I GGTNACC 1 cut(s) 559
EcoO65I GGTNACC 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 83
FaeI CATG 4 cut(s) 94, 112, 448, 615
FaiI YATR 9 cut(s) 62, 92, 110, 123, 446, 476, 549, 570, 613
FatI CATG 4 cut(s) 90, 108, 444, 611
FauI CCCGC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 3 cut(s) 185, 197, 624
FspI TGCGCA 1 cut(s) 610
GlaI GCGC 1 cut(s) 610
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 101
HgaI GACGC 1 cut(s) 272
HhaI GCGC 1 cut(s) 611
Hin1II CATG 4 cut(s) 94, 112, 448, 615
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HinfI GANTC 2 cut(s) 326, 536
HphI GGTGA 2 cut(s) 173, 553
Hpy166II GTNNAC 2 cut(s) 171, 599
Hpy188III TCNNGA 3 cut(s) 428, 488, 578
Hpy8I GTNNAC 2 cut(s) 171, 599
HpyAV CCTTC 3 cut(s) 158, 239, 316
HpyCH4III ACNGT 2 cut(s) 120, 371
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 334
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 4 cut(s) 94, 112, 448, 615
HspAI GCGC 1 cut(s) 609
LguI GCTCTTC 2 cut(s) 183, 522
LmnI GCTCC 3 cut(s) 490, 525, 584
LpnPI CCDG 3 cut(s) 190, 360, 516
Lsp1109I GCAGC 1 cut(s) 200
MaeI CTAG 3 cut(s) 185, 197, 624
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 508, 559
MbiI CCGCTC 1 cut(s) 485
MboII GAAGA 7 cut(s) 20, 23, 47, 92, 170, 323, 509
MluCI AATT 4 cut(s) 20, 83, 391, 432
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 2 cut(s) 427, 503
MnlI CCTC 4 cut(s) 98, 343, 490, 574
MseI TTAA 4 cut(s) 134, 233, 285, 389
NheI GCTAGC 1 cut(s) 623
NlaIII CATG 4 cut(s) 94, 112, 448, 615
NlaIV GGNNCC 3 cut(s) 169, 228, 586
NmuCI GTSAC 2 cut(s) 508, 559
NsbI TGCGCA 1 cut(s) 610
NspI RCATGY 1 cut(s) 615
PaeR7I CTCGAG 1 cut(s) 578
PciSI GCTCTTC 2 cut(s) 183, 522
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 233
Psp1406I AACGTT 1 cut(s) 406
PspEI GGTNACC 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 169, 228, 586
PspPI GGNCC 2 cut(s) 99, 168
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SapI GCTCTTC 2 cut(s) 183, 522
SaqAI TTAA 4 cut(s) 134, 233, 285, 389
SatI GCNGC 1 cut(s) 189
Sau96I GGNCC 2 cut(s) 99, 168
SchI GAGTC 1 cut(s) 335
SetI ASST 8 cut(s) 178, 186, 250, 283, 388, 409, 517, 577
Sfr274I CTCGAG 1 cut(s) 578
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 4 cut(s) 20, 83, 391, 432
SsiI CCGC 2 cut(s) 483, 566
SspMI CTAG 3 cut(s) 185, 197, 624
TaaI ACNGT 2 cut(s) 120, 371
TaiI ACGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 75, 338, 579
TaqII GACCGA 1 cut(s) 547
TasI AATT 4 cut(s) 20, 83, 391, 432
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 4 cut(s) 134, 233, 285, 389
Tru9I TTAA 4 cut(s) 134, 233, 285, 389
TscAI CASTG 2 cut(s) 145, 198
TseFI GTSAC 2 cut(s) 508, 559
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 2 cut(s) 508, 559
TspDTI ATGAA 1 cut(s) 93
TspRI CASTG 2 cut(s) 145, 198
VpaK11BI GGWCC 1 cut(s) 168
VspI ATTAAT 1 cut(s) 233
XapI RAATTY 1 cut(s) 83
XceI RCATGY 1 cut(s) 615
XhoI CTCGAG 1 cut(s) 578
XspI CTAG 3 cut(s) 185, 197, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.