Rw7G038980

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000952
Physical Location & Seq
Reverse (-)
59800002 .. 59815161
15160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G038980.1

Sequence Viewer

Length: 2142 bp
ATGGAAAGTATTCAAGTTGGCTCTAATGCACTTATTTTGGAAGTTCTTAACCGCGATAACTATAAACATTGGAGTTCGCGGTTAGAAACTTATTTGTTGGCTGAAGATCTCTGGGAGGTTGTTGAAGCAATCCCGCCAAAACTCGAAGATGATCAAGCCGAATATAAGGCTTGGACCAAAAAGAATGCAAAGGCTTTGTATGCAATCCAAAATTCATGCGGGAGGGAGCTGTTCTCTTTCATTAGTGAAATTAAGATGGCAAAAGGCGCCTGGGAGGCTTTGGAACAAGAGTGCAAACCTGTGCAAGGATATAATGAGAACAATGCCAACCACTTGATGTGCTATAAATCGTTCATTAAGTCGGTAAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATCCGCAGTGTAGTAGTAGTAAAAGCGGTAGACACATACGGATATATAGCTCTTCATGCAGCAGCCTGGGAAGGGCACGTGCATGTAGTGAAAGAGTTGGTGCTTTTCATGACACAAGAAGATTTGAAAACAAAAAACGATTTTGGTCACACAGCTCTTCATCTAGCAGCACAGAAGGGGCATGTACATATTGTGAACGAGTTGCCCGTGCCGTTGATGGGAGAAGTAAAAGACAATGATGGTGACACAGCTCTTCATATAGCAATCCAGGAAGGGCAGGCCGATGTTGTGAAAGAGTTGGTGTTGTTGATGAGACAAGAAAACTTGAAAATTAAAAACGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAACGTGCCTATGGTCAAAGAGTTCGTTACAAAAGAAAAAGGTGAGAATAATATTGGCAAGGACGACTTTGAGCGTTATATACCGTTTACCACTTATGTGGAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCATGTGGTGCAGTAACAGCAGTAGATCCAAGAGATGACTACCGAGATACTGCTCTTCACGTAGCAGCCCGAAAAGGGCATTTGGATGTTATCAAAGGATTGGTGTTGTTGCTGAAAATGAGACAAGAAGATTTGGAATTGAAAACCGCTGAAGATTTCACAGCTTTTGGTTCTGGTATAACTTTAGGGGTCAGTGAAGAATTCCTCATGGAAAAGGCCAAATACATGGTTGAACAGTACGAGAAAACACTTGGCAGCCTTTTGGAGATACAAAACGCTGACGGGTCCACAGCTCTTCACCTAGCAGTACTGCAGGGGTATGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATCTTACAAAGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAAACAAAAACGGTTCCAATGCTCTTCACCTAGCGGTCGAGAATGAGCATATGCATTTTGCTAGAGAGCTGATGGCATTGATGAGACCAGAGGCTTTGGAAGAAAAAGACGGTGAAGGTTATACAGCTTTAAGTATGGCTATTACATTTTTCAAGGATGGAGACGTTTGCGAAATGGCTAAATACATGGCTGAAAAGAATGTTAAAGTACTTGGCATACCCTCGTCTCCCAATGATCAGATTCCAGTTGTCAGAGCTTGTTCTGTTGATAGATGGAAATCGGCTCGCTATATGTATGCTGTCACTCCACTTGAAGCTCTCGACGGACCCAATGGCAGTAAACTTATTTGCATTTGTCTCGATGCTAGCAAAACGATTGATTTGGCATGGGACTTGCTTCAACGTTGCCCAAATTTGGCCATCACTATCACTAAACCGCCTAAAATCACTACTCCTATGTTGAAATTGGCTCATATGCATTTTGCGTTCTTAAGTGGAACACAACTCACACGCTGGCAAAGGTGGCTTTATGATTGTATGTACATACGAGAAGTTAGTCATGTCAACATTCATGACATATCTATAAATGTTGCTGAATCAAAAGACCATCACAGCAATAAAAGGGATTTCATTTCCTCAGTTATGAGTTTATATAGAAGATTTGCTAAAAGCCTCTCCATATTTTGCGGTATGCTACATCCACACTCCTTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

713

Amino Acids

80.24

Weight (kDa)

5.7

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 8.7e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 6.4e-09 gag-polypeptide of LTR copia-type
Ank_2 PF12796 156 - 239 6.1e-16 Ankyrin repeats (3 copies)
Ank_5 PF13857 217 - 261 1.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 244 - 350 1.8e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 403 - 467 5.8e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 405 - 452 4.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 465 - 543 5e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 266, 1288
AccI GTMKAC 1 cut(s) 444
AccII CGCG 2 cut(s) 54, 79
AclI AACGTT 1 cut(s) 1828
AclWI GGATC 1 cut(s) 965
AcoI YGGCCR 1 cut(s) 1842
AcsI RAATTY 3 cut(s) 211, 1145, 1837
AcuI CTGAAG 2 cut(s) 123, 1116
AcvI CACGTG 1 cut(s) 493
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 5 cut(s) 600, 1184, 1254, 1635, 1967
AfiI CCNNNNNNNGG 5 cut(s) 305, 486, 632, 1020, 1840
AflII CTTAAG 1 cut(s) 1915
AjnI CCWGG 3 cut(s) 269, 479, 681
AleI CACNNNNGTG 1 cut(s) 890
Alw26I GTCTC 8 cut(s) 721, 1060, 1329, 1399, 1504, 1581, 1656, 1787
AlwI GGATC 1 cut(s) 965
AoxI GGCC 3 cut(s) 693, 1161, 1842
ApeKI GCWGC 5 cut(s) 473, 476, 581, 1010, 1200
ApoI RAATTY 3 cut(s) 211, 1145, 1837
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 3 cut(s) 174, 1230, 1751
AsuHPI GGTGA 8 cut(s) 386, 668, 767, 848, 914, 1235, 1445, 1550
AsuNHI GCTAGC 1 cut(s) 1790
AvaII GGWCC 3 cut(s) 174, 1230, 1751
BaeGI GKGCMC 1 cut(s) 492
BalI TGGCCA 1 cut(s) 1844
BanI GGYRCC 2 cut(s) 266, 1288
BbrPI CACGTG 1 cut(s) 493
BbvI GCAGC 5 cut(s) 485, 488, 593, 1022, 1212
BccI CCATC 9 cut(s) 250, 625, 647, 1325, 1492, 1577, 1692, 1853, 2040
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 2 cut(s) 1765, 1799
BciT130I CCWGG 3 cut(s) 271, 481, 683
BclI TGATCA 2 cut(s) 151, 1660
BcoDI GTCTC 8 cut(s) 721, 1060, 1329, 1399, 1504, 1581, 1656, 1787
BfaI CTAG 7 cut(s) 578, 779, 938, 1247, 1457, 1488, 1791
BfmI CTRYAG 1 cut(s) 1256
BfoI RGCGCY 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 1915
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 474, 477, 582, 1011, 1201
BlsI GCNGC 5 cut(s) 475, 478, 583, 1012, 1202
BmcAI AGTACT 2 cut(s) 1254, 1635
Bme1390I CCNGG 3 cut(s) 271, 481, 683
Bme18I GGWCC 3 cut(s) 174, 1230, 1751
BmgT120I GGNCC 3 cut(s) 174, 1230, 1751
BmiI GGNNCC 5 cut(s) 268, 1231, 1290, 1441, 1753
BmrFI CCNGG 3 cut(s) 271, 481, 683
BmsI GCATC 2 cut(s) 745, 1777
BmtI GCTAGC 1 cut(s) 1794
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bsa29I ATCGAT 1 cut(s) 1400
BsaAI YACGTR 2 cut(s) 493, 1006
BsaBI GATNNNNATC 1 cut(s) 1702
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 1504
BsaJI CCNNGG 2 cut(s) 270, 480
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 486, 632, 1020, 1840
Bse1I ACTGG 2 cut(s) 1292, 1670
Bse3DI GCAATG 1 cut(s) 1350
Bse8I GATNNNNATC 1 cut(s) 1702
BseBI CCWGG 3 cut(s) 271, 481, 683
BseCI ATCGAT 1 cut(s) 1400
BseDI CCNNGG 2 cut(s) 270, 480
BseGI GGATG 4 cut(s) 1036, 1276, 1588, 2122
BseJI GATNNNNATC 1 cut(s) 1702
BseLI CCNNNNNNNGG 5 cut(s) 305, 486, 632, 1020, 1840
BseMI GCAATG 1 cut(s) 1350
BseMII CTCAG 1 cut(s) 2076
BseNI ACTGG 2 cut(s) 1292, 1670
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 5 cut(s) 485, 488, 593, 1022, 1212
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 2 cut(s) 54, 79
Bsh1285I CGRYCG 1 cut(s) 1464
BshFI GGCC 3 cut(s) 695, 1163, 1844
BshNI GGYRCC 2 cut(s) 266, 1288
BshVI ATCGAT 1 cut(s) 1400
BsiEI CGRYCG 1 cut(s) 1464
BslFI GGGAC 1 cut(s) 1829
BslI CCNNNNNNNGG 5 cut(s) 305, 486, 632, 1020, 1840
BsmAI GTCTC 8 cut(s) 721, 1060, 1329, 1399, 1504, 1581, 1656, 1787
BsmBI CGTCTC 2 cut(s) 1581, 1656
BsmFI GGGAC 1 cut(s) 1829
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 3 cut(s) 695, 1163, 1844
Bso31I GGTCTC 1 cut(s) 1504
Bsp1286I GDGCHC 1 cut(s) 492
Bsp1407I TGTACA 2 cut(s) 598, 1965
Bsp143I GATC 4 cut(s) 106, 151, 970, 1660
BspANI GGCC 3 cut(s) 695, 1163, 1844
BspCNI CTCAG 1 cut(s) 2075
BspDI ATCGAT 1 cut(s) 1400
BspFNI CGCG 2 cut(s) 54, 79
BspHI TCATGA 2 cut(s) 522, 1996
BspLI GGNNCC 5 cut(s) 268, 1231, 1290, 1441, 1753
BspMAI CTGCAG 1 cut(s) 1260
BspOI GCTAGC 1 cut(s) 1794
BspPI GGATC 1 cut(s) 965
BspQI GCTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
BspT107I GGYRCC 2 cut(s) 266, 1288
BspTI CTTAAG 1 cut(s) 1915
BspTNI GGTCTC 1 cut(s) 1504
BsrDI GCAATG 1 cut(s) 1350
BsrGI TGTACA 2 cut(s) 598, 1965
BsrI ACTGG 2 cut(s) 1292, 1670
BssECI CCNNGG 2 cut(s) 270, 480
BssMI GATC 4 cut(s) 106, 151, 970, 1660
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 3 cut(s) 271, 481, 683
Bst4CI ACNGT 4 cut(s) 879, 1182, 1439, 1538
Bst6I CTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 1915
BstAUI TGTACA 2 cut(s) 598, 1965
BstBAI YACGTR 2 cut(s) 493, 1006
BstC8I GCNNGC 4 cut(s) 693, 1711, 1792, 1940
BstDEI CTNAG 2 cut(s) 929, 2062
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 4 cut(s) 1036, 1276, 1588, 2122
BstFNI CGCG 2 cut(s) 54, 79
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 4 cut(s) 109, 154, 973, 1663
BstMAI GTCTC 8 cut(s) 721, 1060, 1329, 1399, 1504, 1581, 1656, 1787
BstMBI GATC 4 cut(s) 106, 151, 970, 1660
BstMCI CGRYCG 1 cut(s) 1464
BstMWI GCNNNNNNNGC 6 cut(s) 200, 266, 275, 470, 962, 1948
BstNI CCWGG 3 cut(s) 271, 481, 683
BstNSI RCATGY 3 cut(s) 500, 599, 1373
BstSCI CCNGG 3 cut(s) 269, 479, 681
BstSFI CTRYAG 1 cut(s) 1256
BstSLI GKGCMC 1 cut(s) 492
BstUI CGCG 2 cut(s) 54, 79
BstV1I GCAGC 5 cut(s) 485, 488, 593, 1022, 1212
BstX2I RGATCY 2 cut(s) 106, 970
BstXI CCANNNNNNTGG 2 cut(s) 892, 1171
BstYI RGATCY 2 cut(s) 106, 970
Bsu15I ATCGAT 1 cut(s) 1400
BsuRI GGCC 3 cut(s) 695, 1163, 1844
BsuTUI ATCGAT 1 cut(s) 1400
BtsCI GGATG 4 cut(s) 1036, 1276, 1588, 2122
BtsI GCAGTG 2 cut(s) 376, 427
BtsIMutI CAGTG 3 cut(s) 376, 427, 1144
Cac8I GCNNGC 4 cut(s) 693, 1711, 1792, 1940
CciI TCATGA 2 cut(s) 522, 1996
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 174, 1230, 1751
ClaI ATCGAT 1 cut(s) 1400
CseI GACGC 1 cut(s) 1334
Csp6I GTAC 5 cut(s) 599, 1183, 1253, 1634, 1966
CviQI GTAC 5 cut(s) 599, 1183, 1253, 1634, 1966
DdeI CTNAG 2 cut(s) 929, 2062
DinI GGCGCC 1 cut(s) 268
DpnI GATC 4 cut(s) 108, 153, 972, 1662
DpnII GATC 4 cut(s) 106, 151, 970, 1660
EaeI YGGCCR 1 cut(s) 1842
Eam1104I CTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
EarI CTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
Eco31I GGTCTC 1 cut(s) 1504
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 3 cut(s) 174, 1230, 1751
Eco57I CTGAAG 2 cut(s) 123, 1116
Eco72I CACGTG 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoRI GAATTC 1 cut(s) 1145
EcoRII CCWGG 3 cut(s) 269, 479, 681
EcoRV GATATC 1 cut(s) 415
EcoT22I ATGCAT 2 cut(s) 1482, 1905
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
Esp3I CGTCTC 2 cut(s) 1581, 1656
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 1 cut(s) 1829
FauI CCCGC 2 cut(s) 141, 212
FauNDI CATATG 2 cut(s) 1476, 1899
FbaI TGATCA 2 cut(s) 151, 1660
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 5 cut(s) 474, 477, 582, 1011, 1201
FokI GGATG 4 cut(s) 1043, 1283, 1595, 2109
Fsp4HI GCNGC 5 cut(s) 474, 477, 582, 1011, 1201
FspBI CTAG 7 cut(s) 578, 779, 938, 1247, 1457, 1488, 1791
GlaI GCGC 1 cut(s) 268
GluI GCNGC 5 cut(s) 474, 477, 582, 1011, 1201
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 695, 1163, 1844
HgaI GACGC 1 cut(s) 1334
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 267
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 1 cut(s) 1990
HindII GTYRAC 1 cut(s) 1990
HinfI GANTC 2 cut(s) 1666, 2021
HphI GGTGA 8 cut(s) 386, 668, 767, 848, 914, 1235, 1445, 1550
Hpy166II GTNNAC 6 cut(s) 445, 610, 882, 1233, 1766, 1990
Hpy188I TCNGA 2 cut(s) 1665, 1679
Hpy188III TCNNGA 5 cut(s) 523, 1465, 1745, 1784, 1997
Hpy8I GTNNAC 6 cut(s) 445, 610, 882, 1233, 1766, 1990
Hpy99I CGWCG 1 cut(s) 1751
HpyAV CCTTC 7 cut(s) 479, 583, 680, 1301, 1378, 1406, 1535
HpyCH4III ACNGT 4 cut(s) 879, 1182, 1439, 1538
HpyCH4IV ACGT 5 cut(s) 492, 798, 1005, 1590, 1828
HpyF10VI GCNNNNNNNGC 6 cut(s) 200, 266, 275, 470, 962, 1948
HpyF3I CTNAG 2 cut(s) 929, 2062
HpySE526I ACGT 5 cut(s) 492, 798, 1005, 1590, 1828
Hsp92I GRCGYC 1 cut(s) 267
HspAI GCGC 1 cut(s) 267
KasI GGCGCC 1 cut(s) 266
Ksp22I TGATCA 2 cut(s) 151, 1660
Kzo9I GATC 4 cut(s) 106, 151, 970, 1660
LguI GCTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 5 cut(s) 485, 488, 593, 1022, 1212
LweI GCATC 2 cut(s) 745, 1777
MaeI CTAG 7 cut(s) 578, 779, 938, 1247, 1457, 1488, 1791
MaeII ACGT 5 cut(s) 492, 798, 1005, 1590, 1828
MaeIII GTNAC 6 cut(s) 560, 656, 761, 820, 958, 1726
MalI GATC 4 cut(s) 108, 153, 972, 1662
MboI GATC 4 cut(s) 106, 151, 970, 1660
MflI RGATCY 2 cut(s) 106, 970
MhlI GDGCHC 1 cut(s) 492
MlsI TGGCCA 1 cut(s) 1844
MluCI AATT 7 cut(s) 211, 249, 744, 1082, 1145, 1837, 1889
MluNI TGGCCA 1 cut(s) 1844
Mly113I GGCGCC 1 cut(s) 267
MnlI CCTC 8 cut(s) 109, 216, 268, 1160, 1510, 1657, 2071, 2108
Mox20I TGGCCA 1 cut(s) 1844
Mph1103I ATGCAT 2 cut(s) 1482, 1905
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 1 cut(s) 1844
MseI TTAA 9 cut(s) 48, 252, 357, 747, 1347, 1556, 1629, 1916, 2140
MslI CAYNNNNRTG 3 cut(s) 495, 890, 1029
Msp20I TGGCCA 1 cut(s) 1844
MspA1I CMGCKG 1 cut(s) 1094
MspCI CTTAAG 1 cut(s) 1915
MspR9I CCNGG 3 cut(s) 271, 481, 683
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 3 cut(s) 271, 481, 683
MvnI CGCG 2 cut(s) 54, 79
MwoI GCNNNNNNNGC 6 cut(s) 200, 266, 275, 470, 962, 1948
NarI GGCGCC 1 cut(s) 267
NdeI CATATG 2 cut(s) 1476, 1899
NdeII GATC 4 cut(s) 106, 151, 970, 1660
NheI GCTAGC 1 cut(s) 1790
NlaIV GGNNCC 5 cut(s) 268, 1231, 1290, 1441, 1753
NmuCI GTSAC 3 cut(s) 560, 656, 1726
NsiI ATGCAT 2 cut(s) 1482, 1905
NspI RCATGY 3 cut(s) 500, 599, 1373
OliI CACNNNNGTG 1 cut(s) 890
PagI TCATGA 2 cut(s) 522, 1996
PciSI GCTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PctI GAATGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 1666, 2021
PfoI TCCNGGA 1 cut(s) 681
PkrI GCNGC 5 cut(s) 475, 478, 583, 1012, 1202
PluTI GGCGCC 1 cut(s) 270
PmaCI CACGTG 1 cut(s) 493
PmlI CACGTG 1 cut(s) 493
Ppu21I YACGTR 2 cut(s) 493, 1006
Psp1406I AACGTT 1 cut(s) 1828
Psp6I CCWGG 3 cut(s) 269, 479, 681
PspCI CACGTG 1 cut(s) 493
PspGI CCWGG 3 cut(s) 269, 479, 681
PspN4I GGNNCC 5 cut(s) 268, 1231, 1290, 1441, 1753
PspPI GGNCC 3 cut(s) 174, 1230, 1751
PstI CTGCAG 1 cut(s) 1260
PsuI RGATCY 2 cut(s) 106, 970
RsaI GTAC 5 cut(s) 600, 1184, 1254, 1635, 1967
RsaNI GTAC 5 cut(s) 599, 1183, 1253, 1634, 1966
RseI CAYNNNNRTG 3 cut(s) 495, 890, 1029
SapI GCTCTTC 7 cut(s) 471, 576, 672, 777, 1005, 1245, 1455
SaqAI TTAA 9 cut(s) 48, 252, 357, 747, 1347, 1556, 1629, 1916, 2140
SatI GCNGC 5 cut(s) 474, 477, 582, 1011, 1201
Sau3AI GATC 4 cut(s) 106, 151, 970, 1660
Sau96I GGNCC 3 cut(s) 174, 1230, 1751
ScaI AGTACT 2 cut(s) 1254, 1635
ScrFI CCNGG 3 cut(s) 271, 481, 683
SduI GDGCHC 1 cut(s) 492
SfaNI GCATC 2 cut(s) 745, 1777
SfcI CTRYAG 1 cut(s) 1256
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 3 cut(s) 174, 1230, 1751
SmiMI CAYNNNNRTG 3 cut(s) 495, 890, 1029
SmlI CTYRAG 1 cut(s) 1915
SmoI CTYRAG 1 cut(s) 1915
Sse9I AATT 7 cut(s) 211, 249, 744, 1082, 1145, 1837, 1889
SspDI GGCGCC 1 cut(s) 266
SspI AATATT 1 cut(s) 847
SspMI CTAG 7 cut(s) 578, 779, 938, 1247, 1457, 1488, 1791
StyD4I CCNGG 3 cut(s) 269, 479, 681
TaaI ACNGT 4 cut(s) 879, 1182, 1439, 1538
TaiI ACGT 5 cut(s) 495, 801, 1008, 1593, 1831
TaqI TCGA 5 cut(s) 144, 1400, 1464, 1746, 1785
TasI AATT 7 cut(s) 211, 249, 744, 1082, 1145, 1837, 1889
TatI WGTACW 4 cut(s) 598, 1252, 1633, 1965
TfiI GAWTC 2 cut(s) 1666, 2021
Tru1I TTAA 9 cut(s) 48, 252, 357, 747, 1347, 1556, 1629, 1916, 2140
Tru9I TTAA 9 cut(s) 48, 252, 357, 747, 1347, 1556, 1629, 1916, 2140
TscAI CASTG 3 cut(s) 376, 427, 1144
TseFI GTSAC 3 cut(s) 560, 656, 1726
TseI GCWGC 5 cut(s) 473, 476, 581, 1010, 1200
Tsp45I GTSAC 3 cut(s) 560, 656, 1726
TspDTI ATGAA 9 cut(s) 204, 229, 343, 458, 511, 563, 659, 1985, 2044
TspGWI ACGGA 2 cut(s) 468, 1764
TspRI CASTG 3 cut(s) 376, 427, 1144
Vha464I CTTAAG 1 cut(s) 1915
VpaK11BI GGWCC 3 cut(s) 174, 1230, 1751
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 3 cut(s) 211, 1145, 1837
XceI RCATGY 3 cut(s) 500, 599, 1373
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 7 cut(s) 578, 779, 938, 1247, 1457, 1488, 1791
ZrmI AGTACT 2 cut(s) 1254, 1635
Zsp2I ATGCAT 2 cut(s) 1482, 1905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.