RchiOBHm_Chr7g0242961

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000952
Physical Location & Seq
Forward (+)
69258991 .. 69260763
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21774

Sequence Viewer

Length: 1521 bp
ATGACACAAGAAGATTTGAAAACAAAAACCGCACAAGGTCACACAGCTCTTCACGTAGCAGCAGGGATGGGGCATGTGCATGTTGTGAAAGAGTTGCCGGTGCCGTTGATGGGAGATGTAAAAGACAAGGATGGTGACACGGCTCTTCATATAGCAGTCCAAGAAGGGCAGGCCGATGTTGTGAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACTTGAAAATAAAAAACGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAACGTGCCTATGGTCAAAGAGTTAGTTACAAAAGAAAAAGGTGAGAATAATATTGGCGACGACGACTTTGAGCGTTATATACCGTTTACCACCTATGTGCAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCATGCAGTGCAATAACAACAGTAGATCCAAGAGACGACTACCGAGACACTGCTCTTCACCTAGCAGCCCGGGAAGGGCATTTGGATGTTATCAAAGGATTGGTGTTGTTGTTGAAAATGAGACAAGAAGATTTAGAATTGAAAACCGCTGAAGATTTCACAACATTTGGTTCTGATATAACTTTAGGGGTCAGTGAAGAATTCCTCACGGAAAGGGCCAAATACATGGTTGAACAGTGTGAGAGAACACTTGGCAGCCTTTTGGAGATACAAAACGCTGAAGGGTCCACAGCTCTTCACCTAGCAATACTGCAGGGGTATGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATCTTACAAAGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAAACAAAGAGGGTTCCGATGCTCTTCACCGAGCAGCATTGAAGGGATATGTAGATATTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATCTTACAAGGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAAACAAAAACGGTTCCAATGCTCTTCACCAAACGGTCAACAATAGGCATATGCATTTTGCTAAAGAGTTGATGGCATTGATGAGACCAGAGGCTTTGGAACAAAAAGACGGTGAAGGTTATACAGCTTTAAGTATTGCTATTTTATGTTTCAAGGATGGAGACGTTTGCGAAATGGCTAAATACATGGCTGAAAAGAATGTTAAAGTACTTGGCATACCCTCCGCTCCCGATGATGAGATTCCAGTTGTCAGAGCTTGTCGTTCTAGTAAATGGAAGTCGGCTCGCTATATGTATTCTGTCACTCCACTTGAAGTGCTCGAGGGACCCAATGGCAGTGATCTTATTTGCTTTTGTCTCAATTCTAGCAAAACGATTGGTAAGACTACTTTCACTTTTTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

506

Amino Acids

56.11

Weight (kDa)

5.2

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 65 3.2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 69 - 176 1.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 225 - 292 4.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 230 - 278 9.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 300 - 362 3.2e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 300 - 348 8.2e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AccB1I GGYRCC 3 cut(s) 100, 766, 976
AccBSI CCGCTC 1 cut(s) 1340
AciI CCGC 3 cut(s) 30, 570, 1338
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 594, 723
AfaI GTAC 1 cut(s) 1323
AfiI CCNNNNNNNGG 2 cut(s) 110, 498
AgsI TTSAA 8 cut(s) 19, 220, 538, 565, 656, 946, 1267, 1427
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 7 cut(s) 47, 248, 716, 821, 1031, 1241, 1370
AluI AGCT 7 cut(s) 47, 248, 716, 821, 1031, 1241, 1370
Alw21I GWGCWC 1 cut(s) 1434
AlwI GGATC 1 cut(s) 443
Ama87I CYCGRG 2 cut(s) 492, 1433
AoxI GGCC 2 cut(s) 171, 639
ApeKI GCWGC 4 cut(s) 59, 488, 678, 938
ApoI RAATTY 1 cut(s) 623
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AspS9I GGNCC 3 cut(s) 639, 708, 1439
AsuC2I CCSGG 2 cut(s) 493, 494
AsuHPI GGTGA 9 cut(s) 146, 245, 326, 392, 473, 713, 923, 1133, 1238
AvaI CYCGRG 2 cut(s) 492, 1433
AvaII GGWCC 2 cut(s) 708, 1439
BanI GGYRCC 3 cut(s) 100, 766, 976
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 1434
BbvI GCAGC 4 cut(s) 71, 500, 690, 950
BccI CCATC 7 cut(s) 61, 103, 125, 803, 1013, 1180, 1265
BceAI ACGGC 2 cut(s) 88, 156
BcnI CCSGG 2 cut(s) 493, 494
BfaI CTAG 6 cut(s) 257, 416, 485, 725, 1380, 1479
BfmI CTRYAG 1 cut(s) 734
BisI GCNGC 4 cut(s) 60, 489, 679, 939
BlsI GCNGC 4 cut(s) 61, 490, 680, 940
BmcAI AGTACT 1 cut(s) 1323
Bme1390I CCNGG 2 cut(s) 493, 494
Bme18I GGWCC 2 cut(s) 708, 1439
BmeT110I CYCGRG 2 cut(s) 492, 1433
BmgT120I GGNCC 3 cut(s) 639, 708, 1439
BmiI GGNNCC 8 cut(s) 102, 709, 768, 919, 978, 1129, 1440, 1441
BmrFI CCNGG 2 cut(s) 493, 494
BmsI GCATC 2 cut(s) 223, 913
BpiI GAAGAC 1 cut(s) 219
BpuMI CCSGG 2 cut(s) 493, 494
Bsa29I ATCGAT 2 cut(s) 878, 1088
BsaAI YACGTR 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 1192
BsaJI CCNNGG 1 cut(s) 492
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 498
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 3 cut(s) 770, 980, 1358
Bse3DI GCAATG 2 cut(s) 828, 1038
BseCI ATCGAT 2 cut(s) 878, 1088
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 5 cut(s) 72, 136, 514, 754, 1276
BseLI CCNNNNNNNGG 2 cut(s) 110, 498
BseMI GCAATG 2 cut(s) 828, 1038
BseNI ACTGG 3 cut(s) 770, 980, 1358
BseXI GCAGC 4 cut(s) 71, 500, 690, 950
BshFI GGCC 2 cut(s) 173, 641
BshNI GGYRCC 3 cut(s) 100, 766, 976
BshVI ATCGAT 2 cut(s) 878, 1088
BsiHKAI GWGCWC 1 cut(s) 1434
BsiHKCI CYCGRG 2 cut(s) 492, 1433
BsiSI CCGG 2 cut(s) 98, 493
BslFI GGGAC 1 cut(s) 1452
BslI CCNNNNNNNGG 2 cut(s) 110, 498
BsmBI CGTCTC 2 cut(s) 450, 1269
BsmFI GGGAC 1 cut(s) 1452
BsnI GGCC 2 cut(s) 173, 641
Bso31I GGTCTC 1 cut(s) 1192
BsoBI CYCGRG 2 cut(s) 492, 1433
Bsp1286I GDGCHC 1 cut(s) 1434
Bsp143I GATC 2 cut(s) 448, 1453
BspACI CCGC 3 cut(s) 30, 570, 1338
BspANI GGCC 2 cut(s) 173, 641
BspDI ATCGAT 2 cut(s) 878, 1088
BspLI GGNNCC 8 cut(s) 102, 709, 768, 919, 978, 1129, 1440, 1441
BspMAI CTGCAG 1 cut(s) 738
BspPI GGATC 1 cut(s) 443
BspQI GCTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
BspT107I GGYRCC 3 cut(s) 100, 766, 976
BspTNI GGTCTC 1 cut(s) 1192
BsrBI CCGCTC 1 cut(s) 1340
BsrDI GCAATG 2 cut(s) 828, 1038
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 3 cut(s) 770, 980, 1358
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 2 cut(s) 448, 1453
Bst4CI ACNGT 6 cut(s) 357, 445, 660, 1127, 1150, 1226
Bst6I CTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 171, 1399
BstDEI CTNAG 1 cut(s) 407
BstF5I GGATG 5 cut(s) 72, 136, 514, 754, 1276
BstKTI GATC 2 cut(s) 451, 1456
BstMBI GATC 2 cut(s) 448, 1453
BstNSI RCATGY 4 cut(s) 77, 83, 851, 1061
BstSCI CCNGG 2 cut(s) 491, 492
BstSFI CTRYAG 1 cut(s) 734
BstV1I GCAGC 4 cut(s) 71, 500, 690, 950
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 448
Bsu15I ATCGAT 2 cut(s) 878, 1088
BsuRI GGCC 2 cut(s) 173, 641
BsuTUI ATCGAT 2 cut(s) 878, 1088
BtsCI GGATG 5 cut(s) 72, 136, 514, 754, 1276
BtsI GCAGTG 3 cut(s) 436, 471, 1456
BtsIMutI CAGTG 5 cut(s) 436, 471, 622, 665, 1456
Cac8I GCNNGC 2 cut(s) 171, 1399
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 3 cut(s) 639, 708, 1439
Cfr9I CCCGGG 1 cut(s) 492
ClaI ATCGAT 2 cut(s) 878, 1088
CseI GACGC 2 cut(s) 812, 1022
Csp6I GTAC 1 cut(s) 1322
CviAII CATG 7 cut(s) 74, 80, 426, 649, 848, 1058, 1300
CviQI GTAC 1 cut(s) 1322
DdeI CTNAG 1 cut(s) 407
DpnI GATC 2 cut(s) 450, 1455
DpnII GATC 2 cut(s) 448, 1453
Eam1104I CTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
EarI CTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
Eco31I GGTCTC 1 cut(s) 1192
Eco47I GGWCC 2 cut(s) 708, 1439
Eco57I CTGAAG 2 cut(s) 594, 723
Eco88I CYCGRG 2 cut(s) 492, 1433
EcoO109I RGGNCCY 1 cut(s) 1439
EcoRI GAATTC 1 cut(s) 623
EcoT22I ATGCAT 1 cut(s) 1170
Esp3I CGTCTC 2 cut(s) 450, 1269
FaeI CATG 7 cut(s) 77, 83, 429, 652, 851, 1061, 1303
FaqI GGGAC 1 cut(s) 1452
FatI CATG 7 cut(s) 73, 79, 425, 648, 847, 1057, 1299
FauNDI CATATG 1 cut(s) 1164
Fnu4HI GCNGC 4 cut(s) 60, 489, 679, 939
FokI GGATG 5 cut(s) 79, 143, 521, 761, 1283
Fsp4HI GCNGC 4 cut(s) 60, 489, 679, 939
FspBI CTAG 6 cut(s) 257, 416, 485, 725, 1380, 1479
GluI GCNGC 4 cut(s) 60, 489, 679, 939
HaeIII GGCC 2 cut(s) 173, 641
HapII CCGG 2 cut(s) 98, 493
HgaI GACGC 2 cut(s) 812, 1022
Hin1II CATG 7 cut(s) 77, 83, 429, 652, 851, 1061, 1303
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HinfI GANTC 1 cut(s) 1354
HpaII CCGG 2 cut(s) 98, 493
HphI GGTGA 9 cut(s) 146, 245, 326, 392, 473, 713, 923, 1133, 1238
Hpy166II GTNNAC 3 cut(s) 360, 711, 1153
Hpy188I TCNGA 3 cut(s) 598, 922, 1367
Hpy188III TCNNGA 1 cut(s) 1343
Hpy8I GTNNAC 3 cut(s) 360, 711, 1153
Hpy99I CGWCG 2 cut(s) 335, 338
HpyCH4III ACNGT 6 cut(s) 357, 445, 660, 1127, 1150, 1226
HpyCH4IV ACGT 3 cut(s) 54, 276, 1278
HpyF3I CTNAG 1 cut(s) 407
HpySE526I ACGT 3 cut(s) 54, 276, 1278
Hsp92II CATG 7 cut(s) 77, 83, 429, 652, 851, 1061, 1303
KflI GGGWCCC 1 cut(s) 1439
Kzo9I GATC 2 cut(s) 448, 1453
LguI GCTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
LmnI GCTCC 1 cut(s) 1345
Lsp1109I GCAGC 4 cut(s) 71, 500, 690, 950
LweI GCATC 2 cut(s) 223, 913
MaeI CTAG 6 cut(s) 257, 416, 485, 725, 1380, 1479
MaeII ACGT 3 cut(s) 54, 276, 1278
MaeIII GTNAC 5 cut(s) 38, 134, 239, 298, 1414
MalI GATC 2 cut(s) 450, 1455
MbiI CCGCTC 1 cut(s) 1340
MboI GATC 2 cut(s) 448, 1453
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 1434
MluCI AATT 3 cut(s) 560, 623, 1474
MnlI CCTC 5 cut(s) 638, 907, 1198, 1345, 1429
Mph1103I ATGCAT 1 cut(s) 1170
MseI TTAA 4 cut(s) 825, 1035, 1244, 1317
MslI CAYNNNNRTG 3 cut(s) 78, 368, 507
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 2 cut(s) 98, 493
MspR9I CCNGG 2 cut(s) 493, 494
NciI CCSGG 2 cut(s) 493, 494
NdeI CATATG 1 cut(s) 1164
NdeII GATC 2 cut(s) 448, 1453
NlaIII CATG 7 cut(s) 77, 83, 429, 652, 851, 1061, 1303
NlaIV GGNNCC 8 cut(s) 102, 709, 768, 919, 978, 1129, 1440, 1441
NmuCI GTSAC 3 cut(s) 38, 134, 1414
NsiI ATGCAT 1 cut(s) 1170
NspI RCATGY 4 cut(s) 77, 83, 851, 1061
OliI CACNNNNGTG 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 1433
PciSI GCTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
PfeI GAWTC 1 cut(s) 1354
PkrI GCNGC 4 cut(s) 61, 490, 680, 940
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 1439
PsiI TTATAA 1 cut(s) 1515
Psp5II RGGWCCY 1 cut(s) 1439
PspN4I GGNNCC 8 cut(s) 102, 709, 768, 919, 978, 1129, 1440, 1441
PspPI GGNCC 3 cut(s) 639, 708, 1439
PspPPI RGGWCCY 1 cut(s) 1439
PspXI VCTCGAGB 1 cut(s) 1433
PstI CTGCAG 1 cut(s) 738
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 1 cut(s) 1323
RsaNI GTAC 1 cut(s) 1322
RseI CAYNNNNRTG 3 cut(s) 78, 368, 507
SapI GCTCTTC 7 cut(s) 54, 150, 255, 483, 723, 933, 1143
SaqAI TTAA 4 cut(s) 825, 1035, 1244, 1317
SatI GCNGC 4 cut(s) 60, 489, 679, 939
Sau3AI GATC 2 cut(s) 448, 1453
Sau96I GGNCC 3 cut(s) 639, 708, 1439
ScaI AGTACT 1 cut(s) 1323
ScrFI CCNGG 2 cut(s) 493, 494
SduI GDGCHC 1 cut(s) 1434
SfaNI GCATC 2 cut(s) 223, 913
SfcI CTRYAG 1 cut(s) 734
Sfr274I CTCGAG 1 cut(s) 1433
SinI GGWCC 2 cut(s) 708, 1439
SlaI CTCGAG 1 cut(s) 1433
SmaI CCCGGG 1 cut(s) 494
SmiMI CAYNNNNRTG 3 cut(s) 78, 368, 507
SmlI CTYRAG 1 cut(s) 1433
SmoI CTYRAG 1 cut(s) 1433
Sse9I AATT 3 cut(s) 560, 623, 1474
SsiI CCGC 3 cut(s) 30, 570, 1338
SspI AATATT 1 cut(s) 325
SspMI CTAG 6 cut(s) 257, 416, 485, 725, 1380, 1479
StyD4I CCNGG 2 cut(s) 491, 492
TaaI ACNGT 6 cut(s) 357, 445, 660, 1127, 1150, 1226
TaiI ACGT 3 cut(s) 57, 279, 1281
TaqI TCGA 3 cut(s) 878, 1088, 1434
TasI AATT 3 cut(s) 560, 623, 1474
TatI WGTACW 1 cut(s) 1321
TfiI GAWTC 1 cut(s) 1354
Tru1I TTAA 4 cut(s) 825, 1035, 1244, 1317
Tru9I TTAA 4 cut(s) 825, 1035, 1244, 1317
TscAI CASTG 5 cut(s) 436, 478, 622, 665, 1456
TseFI GTSAC 3 cut(s) 38, 134, 1414
TseI GCWGC 4 cut(s) 59, 488, 678, 938
Tsp45I GTSAC 3 cut(s) 38, 134, 1414
TspDTI ATGAA 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 647
TspMI CCCGGG 1 cut(s) 492
TspRI CASTG 5 cut(s) 436, 478, 622, 665, 1456
VpaK11BI GGWCC 2 cut(s) 708, 1439
XapI RAATTY 1 cut(s) 623
XceI RCATGY 4 cut(s) 77, 83, 851, 1061
XhoI CTCGAG 1 cut(s) 1433
XmaI CCCGGG 1 cut(s) 492
XspI CTAG 6 cut(s) 257, 416, 485, 725, 1380, 1479
ZrmI AGTACT 1 cut(s) 1323
Zsp2I ATGCAT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.