RLG00000000860

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000952
Physical Location & Seq
Reverse (-)
6835242 .. 6938088
102847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000860

Sequence Viewer

Length: 2292 bp
ATGAAGAACAAAGGCTTCCTCAGTAAAGACACGTTTGCTTTGATTTCGAGGCGATATGCGAGGGCAATGAAGGTTTCAGAAGCCATTAAAGCTTTTGAGGAAGATGGAGAACGGAATCTACAAGCAGCAGCGTTGCCGAAGATTTCTTCAATTCAACTATTGATAGTTAGCATGGCAAGTACTCCAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTGAAAGCTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCGAAGCAATCGTGAAACCTCCAAAACGATATGATGAGAACAATGTGGACCACTTGGGGCGCTATAAATCGTTCATAGAATCAGTGAGCAGTGGTGACTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATGCGCAGTGTAGTAGTAAGGGCAGTAGATGGAGATGGAGAAATAGCTCTTCATGTAGCAGCCAAGGAAGGGCATGTGCACATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTAAAAACAAAAACCGCTCGTGGACGTCACACAGCTCTTCACCTAGCAGCATGGAAGGGGCATGTGCATGTTGTGAAAGTGTTACCGGTGCCGTTAATGGGAGAAATACAAGACTGGGAAGGTGACACAGCTCTTCATATCGCAGTCCAGGAAGGGAAGGTCGATATTATTAAAGAGTTGGTGCTGTTGATGAGACAAGAAGACTTGAAATTAAAAAATTATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAATGTGCCTATGGTCAAAGAGTTCCTGAGAAAAGAAAAAGGTGAGAATAATATTGGCGAAGACGACTTTGAGCGTTATATACCGTTTACCAATTATGTGGAAAATGGTGATTGGGATCAGGCAAATGAGAGTCTTAGAGAACTGGATAATCCATGTGGTGCAGTAACAGCAGTAGATCCAAGAGACGACTACCGAGATACTGCTCTTCACGCAGCAGCCCGGGAAAGGCATGTGGGTGTTACCAAAGGATTGGTGTTGTTGCTGAAAAAGAGACAAGAAGATTTGGAATTGAAAACCGCTGAGGATTTCACCACTTTTGGTTCTGGTATAACTTTAGGGGTCAGTGAGGAATTCCTCATGGAAAAGGCCAAATACATGGTTGAACAGTACGAGAAAACACTTGGCAGCTGTTTGGAGATACAAAACGCTAGTGGTTACACAGCTCTCCACTTGGCAGTACTGAGCGGGTATGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTTACCGAGCAGTACTGGAGGGGTATGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATCTTACAAGGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAAACAAAGAAGGTTCCAATGCTCTTCACCTAGCGGTCAAAAATGAGCATATGCATTTTGCTAAAGAGTTGATGGCATCGATGAGACCAGAGGCTTGGGAAGAAAAAGATGGTCAAGATCATACAGCTTTAAGTCTGGCTATTCAACTACTCAAGGATGGAGACGTTTGTGAAATGGCTAAATACATGGCTGAAAAGAATGTGAAAGTATTTGGCATACCCTCGGCTCCCGATGATGAGATTCCAGTTGTCAAAGCTCTTCGTTGGAGTAAATGGAAATCGGGTCGCTATATGTATTCTGTCACTCCACTGGAAGCTCTCAAGGGACCCGATGGCAGTTCACTTATTTGCTATTGTCTCAATGCTAGCAAAACGATTGATTTGGCATGGGACTTGCTTCAACGTTGCCCAAATTTGGCCTTCACTATCACTAAATCGTCTAAAACCACTCCTATGTTGAAATTGGCTCGTATGAATTTTGCATTCTTAAGTGGAACACGACTCACACGCTGGCAAAGATGCCAGTTTAGGCCAGGCGAGCTTGATTCTCTGGGTGTTGCTCTCGGTGTGGGGAGAGGGGTTGAGGAAGCTGACATTGATTCGTCGAGTTTCGATCTCGAGCTGTGGTCGTTGTCGATCAATCTGATTGGTGCTCGGCTACATTCCCGTGGTGGTGGGAATGGCAGGCTGGGGTGGGCGTCCTCTGGACTTGATGACGGTGCTGCGAGCACAAGAGGTGATGCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

764

Amino Acids

84.72

Weight (kDa)

5.73

Isoelectric Point (pI)

31.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 73 - 99 8.8e-09 Domain of unknown function (DUF4219)
Ank_2 PF12796 149 - 210 3.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 220 - 283 1.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 443 - 506 9.5e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 574
Acc16I TGCGCA 1 cut(s) 431
AccB1I GGYRCC 3 cut(s) 634, 1300, 1405
AccBSI CCGCTC 2 cut(s) 563, 1272
AccII CGCG 1 cut(s) 250
AciI CCGC 4 cut(s) 561, 1104, 1272, 1577
AclI AACGTT 1 cut(s) 1945
AclWI GGATC 2 cut(s) 930, 977
AcsI RAATTY 3 cut(s) 1157, 1954, 2017
AcuI CTGAAG 1 cut(s) 294
AcyI GRCGYC 2 cut(s) 571, 2240
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 4 cut(s) 181, 1196, 1266, 1371
AfiI CCNNNNNNNGG 4 cut(s) 495, 644, 1032, 1957
AflII CTTAAG 1 cut(s) 2029
AflIII ACRYGT 1 cut(s) 30
AgeI ACCGGT 1 cut(s) 631
AgsI TTSAA 9 cut(s) 150, 155, 256, 754, 1099, 1190, 1688, 1943, 2002
AjnI CCWGG 2 cut(s) 693, 2074
Alw21I GWGCWC 3 cut(s) 507, 2197, 2273
Alw26I GTCTC 9 cut(s) 733, 984, 1072, 1341, 1446, 1516, 1621, 1698, 1904
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 2 cut(s) 930, 977
Ama87I CYCGRG 2 cut(s) 1026, 2159
AoxI GGCC 3 cut(s) 1173, 1959, 2072
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 8 cut(s) 125, 128, 485, 593, 1019, 1022, 1212, 2264
ApoI RAATTY 3 cut(s) 1157, 1954, 2017
AsiGI ACCGGT 1 cut(s) 631
AspLEI GCGC 2 cut(s) 354, 432
AspS9I GGNCC 2 cut(s) 340, 1868
AsuC2I CCSGG 2 cut(s) 1027, 1028
AsuHPI GGTGA 9 cut(s) 398, 578, 680, 779, 860, 926, 1108, 1562, 2291
AsuNHI GCTAGC 1 cut(s) 1907
AvaI CYCGRG 2 cut(s) 1026, 2159
AvaII GGWCC 2 cut(s) 340, 1868
BaeGI GKGCMC 1 cut(s) 507
BanI GGYRCC 3 cut(s) 634, 1300, 1405
BauI CACGAG 1 cut(s) 564
BbsI GAAGAC 2 cut(s) 753, 873
Bbv12I GWGCWC 3 cut(s) 507, 2197, 2273
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 8 cut(s) 137, 140, 497, 605, 1031, 1034, 1224, 2251
BccI CCATC 9 cut(s) 98, 449, 455, 1337, 1442, 1609, 1646, 1694, 1868
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 2 cut(s) 695, 2076
BcnI CCSGG 2 cut(s) 1027, 1028
BcoDI GTCTC 9 cut(s) 733, 984, 1072, 1341, 1446, 1516, 1621, 1698, 1904
BfaI CTAG 5 cut(s) 590, 791, 1236, 1574, 1908
BfoI RGCGCY 1 cut(s) 355
BfrI CTTAAG 1 cut(s) 2029
BglII AGATCT 1 cut(s) 277
BisI GCNGC 8 cut(s) 126, 129, 486, 594, 1020, 1023, 1213, 2265
BlsI GCNGC 8 cut(s) 127, 130, 487, 595, 1021, 1024, 1214, 2266
BmcAI AGTACT 3 cut(s) 181, 1266, 1371
Bme1390I CCNGG 4 cut(s) 695, 1027, 1028, 2076
Bme18I GGWCC 2 cut(s) 340, 1868
BmeT110I CYCGRG 2 cut(s) 1026, 2159
BmgT120I GGNCC 2 cut(s) 340, 1868
BmiI GGNNCC 7 cut(s) 636, 1302, 1407, 1558, 1770, 1869, 1870
BmrFI CCNGG 4 cut(s) 695, 1027, 1028, 2076
BmrI ACTGGG 2 cut(s) 400, 670
BmsI GCATC 3 cut(s) 1628, 2051, 2272
BmtI GCTAGC 1 cut(s) 1911
BmuI ACTGGG 2 cut(s) 400, 670
BpiI GAAGAC 2 cut(s) 753, 873
BpmI CTGGAG 2 cut(s) 168, 1394
Bpu10I CCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 2 cut(s) 1679, 1847
BpuMI CCSGG 2 cut(s) 1027, 1028
Bsa29I ATCGAT 2 cut(s) 1517, 1622
BsaBI GATNNNNATC 1 cut(s) 921
BsaHI GRCGYC 2 cut(s) 571, 2240
BsaI GGTCTC 1 cut(s) 1621
BsaJI CCNNGG 4 cut(s) 489, 1026, 1764, 2209
BsaWI WCCGGW 1 cut(s) 631
Bsc4I CCNNNNNNNGG 4 cut(s) 495, 644, 1032, 1957
Bse118I RCCGGY 1 cut(s) 631
Bse3DI GCAATG 2 cut(s) 72, 1467
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 2 cut(s) 695, 2076
BseCI ATCGAT 2 cut(s) 1517, 1622
BseDI CCNNGG 4 cut(s) 489, 1026, 1764, 2209
BseGI GGATG 3 cut(s) 1288, 1393, 1705
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 4 cut(s) 495, 644, 1032, 1957
BseMI GCAATG 2 cut(s) 72, 1467
BseMII CTCAG 4 cut(s) 34, 824, 1098, 1259
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 8 cut(s) 137, 140, 497, 605, 1031, 1034, 1224, 2251
BseYI CCCAGC 1 cut(s) 2230
BsgI GTGCAG 1 cut(s) 987
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 1175, 1961, 2074
BshNI GGYRCC 3 cut(s) 634, 1300, 1405
BshTI ACCGGT 1 cut(s) 631
BshVI ATCGAT 2 cut(s) 1517, 1622
BsiHKAI GWGCWC 3 cut(s) 507, 2197, 2273
BsiHKCI CYCGRG 2 cut(s) 1026, 2159
BsiSI CCGG 2 cut(s) 632, 1027
BslFI GGGAC 2 cut(s) 1881, 1946
BslI CCNNNNNNNGG 4 cut(s) 495, 644, 1032, 1957
BsmAI GTCTC 9 cut(s) 733, 984, 1072, 1341, 1446, 1516, 1621, 1698, 1904
BsmBI CGTCTC 2 cut(s) 984, 1698
BsmFI GGGAC 2 cut(s) 1881, 1946
BsmI GAATGC 1 cut(s) 2024
BsnI GGCC 3 cut(s) 1175, 1961, 2074
Bso31I GGTCTC 1 cut(s) 1621
BsoBI CYCGRG 2 cut(s) 1026, 2159
Bsp1286I GDGCHC 3 cut(s) 507, 2197, 2273
Bsp143I GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
Bsp68I TCGCGA 1 cut(s) 250
BspACI CCGC 4 cut(s) 561, 1104, 1272, 1577
BspANI GGCC 3 cut(s) 1175, 1961, 2074
BspCNI CTCAG 4 cut(s) 33, 825, 1099, 1260
BspDI ATCGAT 2 cut(s) 1517, 1622
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 7 cut(s) 636, 1302, 1407, 1558, 1770, 1869, 1870
BspOI GCTAGC 1 cut(s) 1911
BspPI GGATC 2 cut(s) 930, 977
BspQI GCTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
BspT107I GGYRCC 3 cut(s) 634, 1300, 1405
BspTI CTTAAG 1 cut(s) 2029
BspTNI GGTCTC 1 cut(s) 1621
BsrBI CCGCTC 2 cut(s) 563, 1272
BsrDI GCAATG 2 cut(s) 72, 1467
BsrFI RCCGGY 1 cut(s) 631
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 4 cut(s) 489, 1026, 1764, 2209
BssMI GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
BssNI GRCGYC 2 cut(s) 571, 2240
BssSI CACGAG 1 cut(s) 564
BssT1I CCWWGG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 695, 2076
Bst4CI ACNGT 4 cut(s) 224, 891, 1194, 2261
Bst6I CTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
BstACI GRCGYC 2 cut(s) 571, 2240
BstAFI CTTAAG 1 cut(s) 2029
BstC8I GCNNGC 5 cut(s) 1909, 2054, 2081, 2228, 2269
BstDEI CTNAG 5 cut(s) 20, 833, 941, 1107, 1268
BstDSI CCRYGG 1 cut(s) 2209
BstF5I GGATG 3 cut(s) 1288, 1393, 1705
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 2 cut(s) 354, 432
BstKTI GATC 7 cut(s) 248, 280, 925, 985, 1663, 2158, 2181
BstMAI GTCTC 9 cut(s) 733, 984, 1072, 1341, 1446, 1516, 1621, 1698, 1904
BstMBI GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
BstMWI GCNNNNNNNGC 5 cut(s) 89, 296, 974, 1016, 2080
BstNI CCWGG 2 cut(s) 695, 2076
BstNSI RCATGY 5 cut(s) 503, 611, 617, 1040, 1490
BstSCI CCNGG 4 cut(s) 693, 1025, 1026, 2074
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 8 cut(s) 137, 140, 497, 605, 1031, 1034, 1224, 2251
BstV2I GAAGAC 2 cut(s) 753, 873
BstX2I RGATCY 2 cut(s) 277, 982
BstXI CCANNNNNNTGG 4 cut(s) 904, 1057, 1183, 1638
BstYI RGATCY 2 cut(s) 277, 982
Bsu15I ATCGAT 2 cut(s) 1517, 1622
BsuRI GGCC 3 cut(s) 1175, 1961, 2074
BsuTUI ATCGAT 2 cut(s) 1517, 1622
BtgI CCRYGG 1 cut(s) 2209
BtsCI GGATG 3 cut(s) 1288, 1393, 1705
BtsI GCAGTG 2 cut(s) 388, 439
BtsIMutI CAGTG 5 cut(s) 381, 388, 439, 1156, 1850
BtuMI TCGCGA 1 cut(s) 250
Cac8I GCNNGC 5 cut(s) 1909, 2054, 2081, 2228, 2269
CfoI GCGC 2 cut(s) 354, 432
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 2 cut(s) 340, 1868
Cfr9I CCCGGG 1 cut(s) 1026
ClaI ATCGAT 2 cut(s) 1517, 1622
CseI GACGC 3 cut(s) 1346, 1451, 2229
Csp6I GTAC 4 cut(s) 180, 1195, 1265, 1370
CspAI ACCGGT 1 cut(s) 631
CviQI GTAC 4 cut(s) 180, 1195, 1265, 1370
DdeI CTNAG 5 cut(s) 20, 833, 941, 1107, 1268
DpnI GATC 7 cut(s) 247, 279, 924, 984, 1662, 2157, 2180
DpnII GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
DraI TTTAAA 1 cut(s) 549
DraIII CACNNNGTG 1 cut(s) 511
Eam1104I CTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
EarI CTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
Eco130I CCWWGG 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 1621
Eco47I GGWCC 2 cut(s) 340, 1868
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 2 cut(s) 1026, 2159
EcoO109I RGGNCCY 1 cut(s) 1868
EcoRI GAATTC 1 cut(s) 1157
EcoRII CCWGG 2 cut(s) 693, 2074
EcoT14I CCWWGG 1 cut(s) 489
EcoT22I ATGCAT 1 cut(s) 1599
ErhI CCWWGG 1 cut(s) 489
Esp3I CGTCTC 2 cut(s) 984, 1698
FaqI GGGAC 2 cut(s) 1881, 1946
FauI CCCGC 1 cut(s) 1265
FauNDI CATATG 1 cut(s) 1593
Fnu4HI GCNGC 8 cut(s) 126, 129, 486, 594, 1020, 1023, 1213, 2265
FokI GGATG 3 cut(s) 1295, 1400, 1712
Fsp4HI GCNGC 8 cut(s) 126, 129, 486, 594, 1020, 1023, 1213, 2265
FspBI CTAG 5 cut(s) 590, 791, 1236, 1574, 1908
FspI TGCGCA 1 cut(s) 431
GlaI GCGC 2 cut(s) 353, 431
GluI GCNGC 8 cut(s) 126, 129, 486, 594, 1020, 1023, 1213, 2265
GsaI CCCAGC 1 cut(s) 2234
GsuI CTGGAG 2 cut(s) 168, 1394
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 3 cut(s) 1175, 1961, 2074
HapII CCGG 2 cut(s) 632, 1027
HgaI GACGC 3 cut(s) 1346, 1451, 2229
HhaI GCGC 2 cut(s) 354, 432
Hin1I GRCGYC 2 cut(s) 571, 2240
Hin6I GCGC 2 cut(s) 352, 430
HinP1I GCGC 2 cut(s) 352, 430
HindIII AAGCTT 2 cut(s) 90, 258
HinfI GANTC 7 cut(s) 115, 371, 937, 1783, 2043, 2087, 2141
HpaII CCGG 2 cut(s) 632, 1027
HphI GGTGA 9 cut(s) 398, 578, 680, 779, 860, 926, 1108, 1562, 2291
Hpy166II GTNNAC 5 cut(s) 340, 505, 569, 894, 1883
Hpy188I TCNGA 2 cut(s) 79, 2187
Hpy8I GTNNAC 5 cut(s) 340, 505, 569, 894, 1883
Hpy99I CGWCG 1 cut(s) 2149
HpyCH4III ACNGT 4 cut(s) 224, 891, 1194, 2261
HpyCH4IV ACGT 4 cut(s) 32, 571, 1707, 1945
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 296, 974, 1016, 2080
HpyF3I CTNAG 5 cut(s) 20, 833, 941, 1107, 1268
HpySE526I ACGT 4 cut(s) 32, 571, 1707, 1945
Hsp92I GRCGYC 2 cut(s) 571, 2240
HspAI GCGC 2 cut(s) 352, 430
KflI GGGWCCC 1 cut(s) 1868
Kzo9I GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
LguI GCTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
LmnI GCTCC 1 cut(s) 1774
Lsp1109I GCAGC 8 cut(s) 137, 140, 497, 605, 1031, 1034, 1224, 2251
LweI GCATC 3 cut(s) 1628, 2051, 2272
MaeI CTAG 5 cut(s) 590, 791, 1236, 1574, 1908
MaeII ACGT 4 cut(s) 32, 571, 1707, 1945
MalI GATC 7 cut(s) 247, 279, 924, 984, 1662, 2157, 2180
MbiI CCGCTC 2 cut(s) 563, 1272
MboI GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
MflI RGATCY 2 cut(s) 277, 982
MhlI GDGCHC 3 cut(s) 507, 2197, 2273
MluCI AATT 9 cut(s) 150, 755, 763, 898, 1094, 1157, 1954, 2003, 2017
MlyI GAGTC 2 cut(s) 946, 2037
MmeI TCCRAC 1 cut(s) 1787
Mph1103I ATGCAT 1 cut(s) 1599
MseI TTAA 8 cut(s) 87, 548, 641, 717, 758, 1464, 1673, 2030
MslI CAYNNNNRTG 4 cut(s) 612, 1041, 1994, 2208
MspA1I CMGCKG 2 cut(s) 1106, 1215
MspCI CTTAAG 1 cut(s) 2029
MspI CCGG 2 cut(s) 632, 1027
MspR9I CCNGG 4 cut(s) 695, 1027, 1028, 2076
Mva1269I GAATGC 1 cut(s) 2024
MvaI CCWGG 2 cut(s) 695, 2076
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 5 cut(s) 89, 296, 974, 1016, 2080
NciI CCSGG 2 cut(s) 1027, 1028
NdeI CATATG 1 cut(s) 1593
NdeII GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
NheI GCTAGC 1 cut(s) 1907
NlaIV GGNNCC 7 cut(s) 636, 1302, 1407, 1558, 1770, 1869, 1870
NmeAIII GCCGAG 2 cut(s) 1745, 2176
NmuCI GTSAC 4 cut(s) 386, 572, 668, 1843
NruI TCGCGA 1 cut(s) 250
NsbI TGCGCA 1 cut(s) 431
NsiI ATGCAT 1 cut(s) 1599
NspI RCATGY 5 cut(s) 503, 611, 617, 1040, 1490
PaeR7I CTCGAG 1 cut(s) 2159
PciSI GCTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 2024
PfeI GAWTC 5 cut(s) 115, 371, 1783, 2087, 2141
PfoI TCCNGGA 1 cut(s) 693
PinAI ACCGGT 1 cut(s) 631
PkrI GCNGC 8 cut(s) 127, 130, 487, 595, 1021, 1024, 1214, 2266
PleI GAGTC 2 cut(s) 945, 2037
PpsI GAGTC 2 cut(s) 945, 2037
PpuMI RGGWCCY 1 cut(s) 1868
Psp1406I AACGTT 1 cut(s) 1945
Psp5II RGGWCCY 1 cut(s) 1868
Psp6I CCWGG 2 cut(s) 693, 2074
PspFI CCCAGC 1 cut(s) 2230
PspGI CCWGG 2 cut(s) 693, 2074
PspN4I GGNNCC 7 cut(s) 636, 1302, 1407, 1558, 1770, 1869, 1870
PspPI GGNCC 2 cut(s) 340, 1868
PspPPI RGGWCCY 1 cut(s) 1868
PsrI GAACNNNNNNTAC 2 cut(s) 102, 134
PsuI RGATCY 2 cut(s) 277, 982
PvuII CAGCTG 1 cut(s) 1215
RruI TCGCGA 1 cut(s) 250
RsaI GTAC 4 cut(s) 181, 1196, 1266, 1371
RsaNI GTAC 4 cut(s) 180, 1195, 1265, 1370
RseI CAYNNNNRTG 4 cut(s) 612, 1041, 1994, 2208
SapI GCTCTTC 7 cut(s) 480, 588, 684, 789, 1017, 1572, 1806
SaqAI TTAA 8 cut(s) 87, 548, 641, 717, 758, 1464, 1673, 2030
SatI GCNGC 8 cut(s) 126, 129, 486, 594, 1020, 1023, 1213, 2265
Sau3AI GATC 7 cut(s) 245, 277, 922, 982, 1660, 2155, 2178
Sau96I GGNCC 2 cut(s) 340, 1868
ScaI AGTACT 3 cut(s) 181, 1266, 1371
SchI GAGTC 2 cut(s) 946, 2037
ScrFI CCNGG 4 cut(s) 695, 1027, 1028, 2076
SduI GDGCHC 3 cut(s) 507, 2197, 2273
SfaNI GCATC 3 cut(s) 1628, 2051, 2272
Sfr274I CTCGAG 1 cut(s) 2159
SinI GGWCC 2 cut(s) 340, 1868
SlaI CTCGAG 1 cut(s) 2159
SmaI CCCGGG 1 cut(s) 1028
SmiMI CAYNNNNRTG 4 cut(s) 612, 1041, 1994, 2208
SmlI CTYRAG 4 cut(s) 1694, 1862, 2029, 2159
SmoI CTYRAG 4 cut(s) 1694, 1862, 2029, 2159
Sse9I AATT 9 cut(s) 150, 755, 763, 898, 1094, 1157, 1954, 2003, 2017
SsiI CCGC 4 cut(s) 561, 1104, 1272, 1577
SspI AATATT 1 cut(s) 859
SspMI CTAG 5 cut(s) 590, 791, 1236, 1574, 1908
StyD4I CCNGG 4 cut(s) 693, 1025, 1026, 2074
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 224, 891, 1194, 2261
TaiI ACGT 4 cut(s) 35, 574, 1710, 1948
TaqI TCGA 9 cut(s) 47, 294, 708, 1517, 1622, 2147, 2154, 2160, 2177
TasI AATT 9 cut(s) 150, 755, 763, 898, 1094, 1157, 1954, 2003, 2017
TatI WGTACW 3 cut(s) 179, 1264, 1369
TfiI GAWTC 5 cut(s) 115, 371, 1783, 2087, 2141
Tru1I TTAA 8 cut(s) 87, 548, 641, 717, 758, 1464, 1673, 2030
Tru9I TTAA 8 cut(s) 87, 548, 641, 717, 758, 1464, 1673, 2030
TscAI CASTG 5 cut(s) 381, 388, 439, 1156, 1857
TseFI GTSAC 4 cut(s) 386, 572, 668, 1843
TseI GCWGC 8 cut(s) 125, 128, 485, 593, 1019, 1022, 1212, 2264
Tsp45I GTSAC 4 cut(s) 386, 572, 668, 1843
TspDTI ATGAA 7 cut(s) 17, 83, 249, 355, 467, 671, 2030
TspGWI ACGGA 1 cut(s) 127
TspMI CCCGGG 1 cut(s) 1026
TspRI CASTG 5 cut(s) 381, 388, 439, 1156, 1857
Vha464I CTTAAG 1 cut(s) 2029
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 340, 1868
XapI RAATTY 3 cut(s) 1157, 1954, 2017
XceI RCATGY 5 cut(s) 503, 611, 617, 1040, 1490
XhoI CTCGAG 1 cut(s) 2159
XmaI CCCGGG 1 cut(s) 1026
XspI CTAG 5 cut(s) 590, 791, 1236, 1574, 1908
ZraI GACGTC 1 cut(s) 572
ZrmI AGTACT 3 cut(s) 181, 1266, 1371
Zsp2I ATGCAT 1 cut(s) 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.