Rorug07G0317800

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000952
Physical Location & Seq
Reverse (-)
32408288 .. 32412481
4194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0317800.1

Sequence Viewer

Length: 1302 bp
ATGACCAGGTATGCAAGTGCAAGTGCAAGGTCGGATGGTCTTCTCCTCCTTTGTAGAGGGAGGGATGCAAACAGTGTGCCATTGGCAAGTGCATATGGCCTTGCCAAACATAGGGATGGCCGTTGGGAATGGGCAATTGCTCCTGGTGTCTCCCCTTCTCCCAGATATCAACATGCAGCAGTTTTTGTTAATGCACGCTTGCATGTTTCCGGAGGGGCCCTTGGTGGTGGGCGCATGGTTGAAGATTCATCAAGTGTTGCAGTGTTGGATACTGCAGCCGGGGTTTGGTGCGATACAAAATCTGTTGTCATGAGTCCAAGGACAGGTAGATACAGCACTGATGCAGCTGGTGGAGATGCTGCAGTTGAATTGACACGGCGTTGCAGGCATGCAGCTGCTGCGGTTGGTGACCTGATATTTATATACGGTGGTCTACGTGGTGGGGTGTTGCTTGATGACCTACTGGTTGCGGAAGATCTGGCTGCTGCAGAAACAACAACTGCAGCCTCGCATGCTGCAGCTGCAGCTGCTCAGTCTAATTTACCACCAGGCCGTTTGCCTGGAAGATATGGATTCATTGATGAAAGAACGAGGCAGATAATGCCTGAAGAGACATGTATGCTGATAGAAGCACTGAAAATGCCTTGCTCCAGGGATCCAGGTGTAGAGTATTTAGTTGAAGCATCAGCAGCAGAAGCTGAGGCAATCAGCGCTACTTTGGCTGCTGCCAAGGCACGACAAGTTAATGGAGAAGTAGAGTTACCTGATAGAGATCGTGGCTCAGAGGCTACCCCGAGCGGGAAGCAAATATCTACCTTGATCAAGCCTGAAAATGCTGGGTCAAATAATATTCCGTCAGCTGGAGTGCGAGTGCATCATAGAGCTGTTAGTTGTTGTAGTTGCAGAGACTGGGGGAGCTTGGGCGGCATGGTGAGGCAGCTTTCAATAGATCAGTTTGAAAATGAAGGCAGGCGGGTTAGCTATGGGACCCCGGAGAGTGCAACTGCAGCAAGGAAGTTATTAGATCGGCAGATGTCCATTAATAGTGTGCCCAAAAAGGTAATAGCACATCTTTTAAAGCCACATGGATGGAAGCCTCCTGTTCGCAGGCAATTTTTCTTGGATTGCAATGAAATAGCAGATCTTTGTGATAGTGCTGAACGGATATTCTCTAGTGAACCAAGTGTCTTACAACTTAGGGCTCCTATCAAGATGTTTGATGATTTACAGCGGCAATTTGACGATCTCATGCGCCTTTTTGGTGAGTATGATTCACCTTCAACTGCTGGGAATATTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

433

Amino Acids

46.36

Weight (kDa)

6.52

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_2 PF07646 54 - 101 2e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 798
AccI GTMKAC 1 cut(s) 433
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 6 cut(s) 401, 470, 798, 924, 973, 1231
AclWI GGATC 2 cut(s) 650, 663
AcoI YGGCCR 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 627
AfeI AGCGCT 1 cut(s) 712
AfiI CCNNNNNNNGG 5 cut(s) 111, 285, 323, 798, 860
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 6 cut(s) 242, 368, 680, 945, 959, 1281
AjnI CCWGG 6 cut(s) 5, 142, 547, 559, 650, 658
Alw26I GTCTC 3 cut(s) 154, 605, 900
AlwI GGATC 2 cut(s) 650, 663
AlwNI CAGNNNCTG 3 cut(s) 398, 698, 909
Ama87I CYCGRG 1 cut(s) 793
Aor13HI TCCGGA 1 cut(s) 209
Aor51HI AGCGCT 1 cut(s) 712
AoxI GGCC 4 cut(s) 97, 118, 216, 550
ApaI GGGCCC 1 cut(s) 220
AseI ATTAAT 1 cut(s) 1041
AspLEI GCGC 3 cut(s) 234, 713, 1254
AspS9I GGNCC 3 cut(s) 216, 217, 987
AsuC2I CCSGG 2 cut(s) 280, 992
AsuHPI GGTGA 4 cut(s) 419, 943, 1266, 1274
AvaI CYCGRG 1 cut(s) 793
AvaII GGWCC 1 cut(s) 987
BaeGI GKGCMC 2 cut(s) 220, 1053
BamHI GGATCC 1 cut(s) 655
BanII GRGCYC 2 cut(s) 220, 1204
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BbsI GAAGAC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 699
BccI CCATC 3 cut(s) 29, 110, 1083
BceAI ACGGC 3 cut(s) 105, 392, 537
BciT130I CCWGG 6 cut(s) 7, 144, 549, 561, 652, 660
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 2 cut(s) 280, 992
BcoDI GTCTC 3 cut(s) 154, 605, 900
BfaI CTAG 1 cut(s) 1173
BfmI CTRYAG 7 cut(s) 273, 360, 486, 501, 516, 522, 1005
BfoI RGCGCY 1 cut(s) 714
BfuI GTATCC 1 cut(s) 262
BglII AGATCT 2 cut(s) 475, 1141
Bme1390I CCNGG 8 cut(s) 7, 144, 280, 549, 561, 652, 660, 992
Bme18I GGWCC 1 cut(s) 987
BmeT110I CYCGRG 1 cut(s) 793
BmgT120I GGNCC 3 cut(s) 216, 217, 987
BmiI GGNNCC 6 cut(s) 217, 218, 657, 988, 989, 1203
BmrFI CCNGG 8 cut(s) 7, 144, 280, 549, 561, 652, 660, 992
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 5 cut(s) 55, 331, 346, 692, 883
BmuI ACTGGG 1 cut(s) 919
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 634, 882
Bpu10I CCTNAGC 1 cut(s) 699
BpuMI CCSGG 2 cut(s) 280, 992
BsaAI YACGTR 1 cut(s) 437
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 6 cut(s) 220, 279, 317, 651, 729, 990
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 285, 323, 798, 860
Bse1I ACTGG 2 cut(s) 468, 914
Bse3DI GCAATG 1 cut(s) 1135
Bse8I GATNNNNATC 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 209
BseBI CCWGG 6 cut(s) 7, 144, 549, 561, 652, 660
BseDI CCNNGG 6 cut(s) 220, 279, 317, 651, 729, 990
BseGI GGATG 4 cut(s) 40, 70, 121, 1094
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 5 cut(s) 111, 285, 323, 798, 860
BseMI GCAATG 1 cut(s) 1135
BseMII CTCAG 3 cut(s) 545, 690, 795
BseNI ACTGG 2 cut(s) 468, 914
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 220, 1053
BseYI CCCAGC 2 cut(s) 836, 1286
BshFI GGCC 4 cut(s) 99, 120, 218, 552
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 3 cut(s) 210, 279, 992
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 5 cut(s) 111, 285, 323, 798, 860
BsmAI GTCTC 3 cut(s) 154, 605, 900
BsmFI GGGAC 1 cut(s) 1000
BsnI GGCC 4 cut(s) 99, 120, 218, 552
BsoBI CYCGRG 1 cut(s) 793
Bsp120I GGGCCC 1 cut(s) 216
Bsp1286I GDGCHC 3 cut(s) 220, 1053, 1204
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
BspACI CCGC 6 cut(s) 401, 470, 798, 924, 973, 1231
BspANI GGCC 4 cut(s) 99, 120, 218, 552
BspCNI CTCAG 3 cut(s) 544, 691, 794
BspEI TCCGGA 1 cut(s) 209
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 6 cut(s) 217, 218, 657, 988, 989, 1203
BspMAI CTGCAG 7 cut(s) 277, 364, 490, 505, 520, 526, 1009
BspPI GGATC 2 cut(s) 650, 663
BsrBI CCGCTC 1 cut(s) 798
BsrDI GCAATG 1 cut(s) 1135
BsrI ACTGG 2 cut(s) 468, 914
BssECI CCNNGG 6 cut(s) 220, 279, 317, 651, 729, 990
BssMI GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
BssT1I CCWWGG 3 cut(s) 220, 317, 729
Bst2UI CCWGG 6 cut(s) 7, 144, 549, 561, 652, 660
Bst4CI ACNGT 2 cut(s) 74, 428
Bst6I CTCTTC 1 cut(s) 603
BstAPI GCANNNNNTGC 2 cut(s) 398, 601
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 7 cut(s) 196, 200, 386, 390, 513, 971, 1109
BstDEI CTNAG 4 cut(s) 531, 699, 781, 1196
BstEII GGTNACC 1 cut(s) 407
BstF5I GGATG 4 cut(s) 40, 70, 121, 1094
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 3 cut(s) 234, 713, 1254
BstKTI GATC 8 cut(s) 478, 658, 775, 822, 952, 1027, 1144, 1246
BstMAI GTCTC 3 cut(s) 154, 605, 900
BstMBI GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
BstNI CCWGG 6 cut(s) 7, 144, 549, 561, 652, 660
BstNSI RCATGY 5 cut(s) 176, 206, 392, 515, 618
BstPI GGTNACC 1 cut(s) 407
BstSCI CCNGG 8 cut(s) 5, 142, 278, 547, 559, 650, 658, 990
BstSFI CTRYAG 7 cut(s) 273, 360, 486, 501, 516, 522, 1005
BstSLI GKGCMC 2 cut(s) 220, 1053
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 3 cut(s) 475, 655, 1141
BstXI CCANNNNNNTGG 1 cut(s) 1089
BstYI RGATCY 3 cut(s) 475, 655, 1141
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 4 cut(s) 99, 120, 218, 552
BtsCI GGATG 4 cut(s) 40, 70, 121, 1094
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 4 cut(s) 79, 267, 336, 632
Cac8I GCNNGC 7 cut(s) 196, 200, 386, 390, 513, 971, 1109
CaiI CAGNNNCTG 3 cut(s) 398, 698, 909
CciI TCATGA 1 cut(s) 309
CfoI GCGC 3 cut(s) 234, 713, 1254
Cfr13I GGNCC 3 cut(s) 216, 217, 987
CsiI ACCWGGT 1 cut(s) 5
DdeI CTNAG 4 cut(s) 531, 699, 781, 1196
DpnI GATC 8 cut(s) 477, 657, 774, 821, 951, 1026, 1143, 1245
DpnII GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
DraI TTTAAA 1 cut(s) 1077
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 3 cut(s) 220, 317, 729
Eco24I GRGCYC 2 cut(s) 220, 1204
Eco32I GATATC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 987
Eco47III AGCGCT 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 407
EcoO109I RGGNCCY 3 cut(s) 216, 217, 987
EcoO65I GGTNACC 1 cut(s) 407
EcoRII CCWGG 6 cut(s) 5, 142, 547, 559, 650, 658
EcoRV GATATC 1 cut(s) 167
EcoT14I CCWWGG 3 cut(s) 220, 317, 729
EcoT38I GRGCYC 2 cut(s) 220, 1204
ErhI CCWWGG 3 cut(s) 220, 317, 729
FaqI GGGAC 1 cut(s) 1000
FauI CCCGC 2 cut(s) 791, 966
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 819
FblI GTMKAC 1 cut(s) 433
FokI GGATG 4 cut(s) 47, 77, 128, 1101
FriOI GRGCYC 2 cut(s) 220, 1204
FspBI CTAG 1 cut(s) 1173
GlaI GCGC 3 cut(s) 233, 712, 1253
GsaI CCCAGC 2 cut(s) 840, 1290
GsuI CTGGAG 2 cut(s) 634, 882
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 4 cut(s) 99, 120, 218, 552
HapII CCGG 3 cut(s) 210, 279, 992
HhaI GCGC 3 cut(s) 234, 713, 1254
Hin6I GCGC 3 cut(s) 232, 711, 1252
HinP1I GCGC 3 cut(s) 232, 711, 1252
HinfI GANTC 4 cut(s) 245, 313, 573, 1271
HpaII CCGG 3 cut(s) 210, 279, 992
HphI GGTGA 4 cut(s) 419, 943, 1266, 1274
Hpy166II GTNNAC 2 cut(s) 434, 1178
Hpy188I TCNGA 2 cut(s) 34, 784
Hpy188III TCNNGA 3 cut(s) 210, 310, 1210
Hpy8I GTNNAC 2 cut(s) 434, 1178
HpyAV CCTTC 3 cut(s) 165, 959, 1287
HpyCH4III ACNGT 2 cut(s) 74, 428
HpyCH4IV ACGT 1 cut(s) 436
HpyF3I CTNAG 4 cut(s) 531, 699, 781, 1196
HpySE526I ACGT 1 cut(s) 436
HspAI GCGC 3 cut(s) 232, 711, 1252
KflI GGGWCCC 1 cut(s) 987
Kpn2I TCCGGA 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
LmnI GCTCC 4 cut(s) 145, 653, 915, 1207
LweI GCATC 5 cut(s) 55, 331, 346, 692, 883
MabI ACCWGGT 1 cut(s) 5
MaeI CTAG 1 cut(s) 1173
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 2 cut(s) 407, 759
MalI GATC 8 cut(s) 477, 657, 774, 821, 951, 1026, 1143, 1245
MbiI CCGCTC 1 cut(s) 798
MboI GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
MboII GAAGA 5 cut(s) 32, 254, 485, 576, 620
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 3 cut(s) 475, 655, 1141
MhlI GDGCHC 3 cut(s) 220, 1053, 1204
MluCI AATT 5 cut(s) 135, 368, 538, 1112, 1235
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 2 cut(s) 12, 246
MroI TCCGGA 1 cut(s) 209
MseI TTAA 4 cut(s) 189, 744, 1041, 1076
MslI CAYNNNNRTG 2 cut(s) 114, 1087
MspA1I CMGCKG 6 cut(s) 347, 395, 521, 527, 860, 1231
MspI CCGG 3 cut(s) 210, 279, 992
MspR9I CCNGG 8 cut(s) 7, 144, 280, 549, 561, 652, 660, 992
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 6 cut(s) 7, 144, 549, 561, 652, 660
NciI CCSGG 2 cut(s) 280, 992
NdeI CATATG 1 cut(s) 94
NdeII GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
NlaIV GGNNCC 6 cut(s) 217, 218, 657, 988, 989, 1203
NmuCI GTSAC 1 cut(s) 407
NspI RCATGY 5 cut(s) 176, 206, 392, 515, 618
PaeI GCATGC 2 cut(s) 392, 515
PagI TCATGA 1 cut(s) 309
PciI ACATGT 1 cut(s) 614
PfeI GAWTC 3 cut(s) 245, 573, 1271
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Ppu21I YACGTR 1 cut(s) 437
PpuMI RGGWCCY 1 cut(s) 987
PscI ACATGT 1 cut(s) 614
PshBI ATTAAT 1 cut(s) 1041
Psp5II RGGWCCY 1 cut(s) 987
Psp6I CCWGG 6 cut(s) 5, 142, 547, 559, 650, 658
PspEI GGTNACC 1 cut(s) 407
PspFI CCCAGC 2 cut(s) 836, 1286
PspGI CCWGG 6 cut(s) 5, 142, 547, 559, 650, 658
PspN4I GGNNCC 6 cut(s) 217, 218, 657, 988, 989, 1203
PspOMI GGGCCC 1 cut(s) 216
PspPI GGNCC 3 cut(s) 216, 217, 987
PspPPI RGGWCCY 1 cut(s) 987
PstI CTGCAG 7 cut(s) 277, 364, 490, 505, 520, 526, 1009
PstNI CAGNNNCTG 3 cut(s) 398, 698, 909
PsuI RGATCY 3 cut(s) 475, 655, 1141
PvuII CAGCTG 5 cut(s) 347, 395, 521, 527, 860
RseI CAYNNNNRTG 2 cut(s) 114, 1087
SaqAI TTAA 4 cut(s) 189, 744, 1041, 1076
Sau3AI GATC 8 cut(s) 475, 655, 772, 819, 949, 1024, 1141, 1243
Sau96I GGNCC 3 cut(s) 216, 217, 987
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 8 cut(s) 7, 144, 280, 549, 561, 652, 660, 992
SduI GDGCHC 3 cut(s) 220, 1053, 1204
SexAI ACCWGGT 1 cut(s) 5
SfaNI GCATC 5 cut(s) 55, 331, 346, 692, 883
SfcI CTRYAG 7 cut(s) 273, 360, 486, 501, 516, 522, 1005
SinI GGWCC 1 cut(s) 987
SmiMI CAYNNNNRTG 2 cut(s) 114, 1087
SphI GCATGC 2 cut(s) 392, 515
Sse9I AATT 5 cut(s) 135, 368, 538, 1112, 1235
SsiI CCGC 6 cut(s) 401, 470, 798, 924, 973, 1231
SspI AATATT 2 cut(s) 850, 1294
SspMI CTAG 1 cut(s) 1173
StyD4I CCNGG 8 cut(s) 5, 142, 278, 547, 559, 650, 658, 990
StyI CCWWGG 3 cut(s) 220, 317, 729
TaaI ACNGT 2 cut(s) 74, 428
TaiI ACGT 1 cut(s) 439
TasI AATT 5 cut(s) 135, 368, 538, 1112, 1235
TauI GCSGC 2 cut(s) 927, 1234
TfiI GAWTC 3 cut(s) 245, 573, 1271
Tru1I TTAA 4 cut(s) 189, 744, 1041, 1076
Tru9I TTAA 4 cut(s) 189, 744, 1041, 1076
TscAI CASTG 4 cut(s) 79, 267, 343, 639
TseFI GTSAC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 5 cut(s) 237, 565, 597, 978, 1146
TspGWI ACGGA 2 cut(s) 843, 1177
TspRI CASTG 4 cut(s) 79, 267, 343, 639
VpaK11BI GGWCC 1 cut(s) 987
VspI ATTAAT 1 cut(s) 1041
XceI RCATGY 5 cut(s) 176, 206, 392, 515, 618
XmiI GTMKAC 1 cut(s) 433
XspI CTAG 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.