Rw7G039450

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000952
Physical Location & Seq
Forward (+)
60776924 .. 60783599
6676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039450.1

Sequence Viewer

Length: 681 bp
ATGGAAAGTATTCAAGTTGGCTCTAATGCACTTATTTTGGAAGTTCTTAACCGCGATAACTATAAACATTGGAGTTCGCGGTTAGAAACTTATTTGTTGGCTGAAGATCTCTGGGAGGTTGTTGAAGCAATCCCGCCAAAACTCGAAGATGATCAAGCCGAATATAAGGCTTGGACCAAAAAGAATGCAAAGGCTTTGTATGCAATCCAAAATTCATGCGGGAGGGAGCTGTTCTCTTTCATAAGTGAAATTAAGATGGCAAAAGGCGCCTGGGAGGCTTTGGAACGAGAGTGCAAACCTGTGCAAGGATATGATGAGAACAATGCCGACCACTTAGTGCACTATGAATCGTTCATAGAATCATTGAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATGTCCGTAGTGTATCAGTAGTAAAAACAGTATACAGATATGGCGGAGAAATAGCTCTTCATGTAGCAGCCAAGGAAGGGCATGCACATATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTGAAAACAAAAACCGCACAAGCACCTATTGAGGAACAGCAAATAGCAGAGCTTGGAACTAAGGTGGATAAGATCCAAGGAGCATATTACTGGATTATGCAAGCTTCTCAACCAGAACAGCGAAAGTTTAACGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

226

Amino Acids

25.73

Weight (kDa)

5.35

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 97 2.4e-09 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 444
AccII CGCG 2 cut(s) 54, 79
AciI CCGC 6 cut(s) 52, 79, 134, 219, 456, 555
AclWI GGATC 1 cut(s) 607
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 305, 489
AgsI TTSAA 3 cut(s) 14, 125, 544
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 4 cut(s) 229, 467, 592, 644
AluI AGCT 4 cut(s) 229, 467, 592, 644
Alw21I GWGCWC 1 cut(s) 342
Alw44I GTGCAC 1 cut(s) 338
AlwI GGATC 1 cut(s) 607
ApaLI GTGCAC 1 cut(s) 338
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 386
AvaII GGWCC 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 342
BanI GGYRCC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 491
BccI CCATC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 271
BclI TGATCA 1 cut(s) 151
BfoI RGCGCY 1 cut(s) 270
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 106
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 271
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 270, 483, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 489
Bse1I ACTGG 1 cut(s) 635
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 3 cut(s) 270, 483, 616
BseLI CCNNNNNNNGG 2 cut(s) 305, 489
BseNI ACTGG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 1 cut(s) 491
Bsh1236I CGCG 2 cut(s) 54, 79
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 305, 489
BsmI GAATGC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 3 cut(s) 106, 151, 612
BspACI CCGC 6 cut(s) 52, 79, 134, 219, 456, 555
BspFNI CGCG 2 cut(s) 54, 79
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 607
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 266
BsrI ACTGG 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 270, 483, 616
BssMI GATC 3 cut(s) 106, 151, 612
BssNAI GTATAC 1 cut(s) 445
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 483, 616
Bst1107I GTATAC 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 495, 642
BstDEI CTNAG 2 cut(s) 334, 600
BstENI CCTNNNNNAGG 1 cut(s) 303
BstFNI CGCG 2 cut(s) 54, 79
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 3 cut(s) 109, 154, 615
BstMBI GATC 3 cut(s) 106, 151, 612
BstMWI GCNNNNNNNGC 3 cut(s) 200, 266, 275
BstNI CCWGG 1 cut(s) 271
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 1 cut(s) 269
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 2 cut(s) 54, 79
BstV1I GCAGC 1 cut(s) 491
BstX2I RGATCY 2 cut(s) 106, 612
BstYI RGATCY 2 cut(s) 106, 612
BstZ17I GTATAC 1 cut(s) 445
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 495, 642
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 174
CviAII CATG 3 cut(s) 216, 473, 494
DdeI CTNAG 2 cut(s) 334, 600
DinI GGCGCC 1 cut(s) 268
DpnI GATC 3 cut(s) 108, 153, 614
DpnII GATC 3 cut(s) 106, 151, 612
DraIII CACNNNGTG 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EciI GGCGGA 1 cut(s) 471
Eco130I CCWWGG 2 cut(s) 483, 616
Eco47I GGWCC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 2 cut(s) 483, 616
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 2 cut(s) 483, 616
FaeI CATG 3 cut(s) 219, 476, 497
FatI CATG 3 cut(s) 215, 472, 493
FauI CCCGC 2 cut(s) 141, 212
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 480
GlaI GCGC 1 cut(s) 268
GluI GCNGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 270
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 219, 476, 497
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 2 cut(s) 347, 359
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 340, 445
Hpy188III TCNNGA 1 cut(s) 505
Hpy8I GTNNAC 2 cut(s) 340, 445
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 9 cut(s) 29, 188, 203, 294, 304, 340, 497, 520, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 266, 275
HpyF3I CTNAG 2 cut(s) 334, 600
HpySE526I ACGT 1 cut(s) 672
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 219, 476, 497
HspAI GCGC 1 cut(s) 267
KasI GGCGCC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 106, 151, 612
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 226, 620
LpnPI CCDG 6 cut(s) 97, 256, 283, 312, 616, 666
Lsp1109I GCAGC 1 cut(s) 491
MaeII ACGT 1 cut(s) 672
MalI GATC 3 cut(s) 108, 153, 614
MboI GATC 3 cut(s) 106, 151, 612
MboII GAAGA 4 cut(s) 116, 158, 461, 548
MflI RGATCY 2 cut(s) 106, 612
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 2 cut(s) 211, 249
Mly113I GGCGCC 1 cut(s) 267
MnlI CCTC 4 cut(s) 109, 216, 268, 565
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 3 cut(s) 48, 252, 669
MspR9I CCNGG 1 cut(s) 271
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 2 cut(s) 54, 79
MwoI GCNNNNNNNGC 3 cut(s) 200, 266, 275
NarI GGCGCC 1 cut(s) 267
NdeII GATC 3 cut(s) 106, 151, 612
NlaIII CATG 3 cut(s) 219, 476, 497
NlaIV GGNNCC 1 cut(s) 268
NspI RCATGY 1 cut(s) 497
PaeI GCATGC 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 474
PctI GAATGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 347, 359
PkrI GCNGC 1 cut(s) 481
PluTI GGCGCC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 2 cut(s) 106, 612
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 3 cut(s) 48, 252, 669
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 3 cut(s) 106, 151, 612
Sau96I GGNCC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 1 cut(s) 342
SetI ASST 9 cut(s) 120, 231, 301, 469, 568, 594, 606, 646, 675
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 1 cut(s) 174
SphI GCATGC 1 cut(s) 497
Sse9I AATT 2 cut(s) 211, 249
SsiI CCGC 6 cut(s) 52, 79, 134, 219, 456, 555
SspDI GGCGCC 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 2 cut(s) 483, 616
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 675
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 211, 249
TfiI GAWTC 2 cut(s) 347, 359
Tru1I TTAA 3 cut(s) 48, 252, 669
Tru9I TTAA 3 cut(s) 48, 252, 669
TscAI CASTG 1 cut(s) 376
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 5 cut(s) 204, 229, 343, 360, 461
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 1 cut(s) 376
VneI GTGCAC 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 174
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.