Rh1CG186500

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000952
Physical Location & Seq
Reverse (-)
40690932 .. 40695399
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG186500.1

Sequence Viewer

Length: 2754 bp
ATGGCAAGTACTCCATTTTGCTCTAATGCACTTATTTTGGAAGTTCTTGACCGCAATAACTATGAAAATTGGAAACCGCGGCTAGAAACTTATTTGTTGGCTAAAGATCTATGGGAGGTTATTGAAGCAACTGAACCTACAAAACTCAAAGATAATGAAGCGGAGTATAAAGCTTGGAGAAAAAAGAATGCCAAGGCTTTATATGCAATGCAAAATTCATGCGGGCCGGAGATGTTTTCTTTTATAAGAGATATCAAAACGGCCAAAGGTGCTTGGGAGGCTTTGGAACAAGAGTGCAAAGTTGTGCAAGGATATGATGAGAACAATGCCAACCACTTGTTGCGCTATCGATTGTTCATCAAATCTGTAAGCGGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATTCGCAGTGTAGTAGTAAGGGCAGTAGATGGAGATGGAGAAAGGGCTCTTCATGTGGCAGCCATGGAAGGGCACGCGCATATTGTGAAAGAGTTGGTGCATTTAATTACACCAGAAGAATTGAAAACGAAATCCGATTTTGGTCAAACAGCTCTTCACGTAGCAGCAGCGATGGGTCACATGAATGTTGTGAAAGAGTTGCTGGTGTGGCTTATGGGGGAAGTACAAGACGATGATGGTGATACAGCTCTTCACATAGCAGCTGAGGAAGGGCAGGCTGATATTGTGAAAGAGTTGGTGCTGTTGATGAGACCAGAAGACTTGAAAATTAAAAACCATGCTGGTTACACAGCTCTTCACGTAGCAGTCAGCAAGGGAAATGTGCCTATGGTCAAAGAGTTTGTGACAAGAGAAAATGGTGGGAATAATATTGGTGAGGACGATTTTGATCAATATATACCGTTTATCACCAATGTGACATGTGGTGATTGGAATAAGGCAAATGAGTGTCTTAGAAAACTAGACCATCCTTGCAACGCAATAGAAGCAAGGAATCCAAGAAACTATGGAGAGACTGCTCTTCATGTAGCAGCCAAAAAAGGGTGCGTGAAAGTTATCAAAGAATTGGTATCGTTGCTAAAAAAGAGACAAGAAGAATTGAAACTGAAAACCACGGAAGAAATTACAGCTTTTGGTTGTCGTATACATTTAGATGTCAGTGAAGGAGTTGTCATGAAAAAGGCGAAATACATGGTTGAACAATACGAGAAAACACTTGGCAGTATTCTGGAGATACAGAACACATATGGTGTCACAGCTCTTCACGTTGCAGTAGTGGAGGGATACGTGGATATAGTAAAAGAGTTGGCGCCTCTAATGAGACAAGAAGGTTTGGAATTAAAACAATCCGATGGTTCCACGGCCCTTCACCTAGCAGTATCAAGGGGGAATGCAGACATTGTGAAAGAGTTGGTGCCATTTATGAGAAAAGAAGGTCTAGAAATAAAAGACACTAATGGAGACACAGCTCTTAACAGGGCATTCTTGAGAGGCGTGAATAAGGATATTGTGAAGGAGTTGATACAATCGATGAGAGCCGAAGGGTTCGAAGTAAAAAATAACTTGGGTTCCAATGCTCTTCACATGGCAGTCATGGATACGTATATGCATTTCAAAGAGTTGACGTCATTGATGAGACCAGAGGCTTTGGAAGAAAAAGACGGTCAAGGTTGTATAGCTTTAAGTTCCCTTATCAAACGTTTCAAGGATGAAGACGTTTGCAAAATGGCAAAACACATGGCTACAAAAAATGTTAACGTATTTGGCATACCCTCGGCTCCTTATGATGACATTCCAGTTGTTGTGGCTCTTCGTTTGAGCAAATGGGAATCGGCTCGCTATCTCTATTCCGTCACTCCACTTGAAGTTCTCAACGGACCCGATGGCTGTGCATTTCTTTTCTATTGTATAAATAGTAGCAAAACGATTGATCTGGCATGGGATTTGCTTCAACATTGTCCAAACTTGGCCATGACTATAAATGAAAGGACTAAAACTATTCCTATGTTGGGATTGGCTCGTATGAGTTATGCATTCTTAAGTGGAAAACAACTGACATGCTGGCAAAGGTGGATTTATAATCGTATACACATAAGAGTAGTTGCTCAAGTGAACATTCGTGATGTTTCTATAGATGTTGCCGAATCAGAAGATGATCAGGACAATAAAAGGGACTTCATTTCCTCAGTTATCAATTTATTTAGAAGATTTGCTAAAGGTCTCCGTAAACTTTGCGGAATCCAGCGTATATATAAAATGAAATCGACACATGAATGGATCTTCGAATTTCTGCGATGTATGGGCGAAGCAACAAAAAATAATAATCTCACATCAGAACAACTAGACATGGTAAAAACGTCAATGTTCAAAGCTATACAACAAGGGCATTTTGAGTTTGTTACACATCTTTGTAAAGCAAATCCAGAACTCCTGGACCAAATTAACCGTCAAGCAGGCAAAGGCATATTTCATCTTGCCATTGAAACATGTCAAAAAGAGGTTTATAGTCTTATCTATGGGCTCAAGGAAGAAGAGAGACTTCAGATTGGAATTGGTGTAGTGGACATGGACAATAATTTGCTACATTATGCCGCAAACTTATCCTCGCTCCCACAGTTTGATCATATTCAAGATGCAGGTTTACAAATGCAAAGAGAATTGCAATGGTTTAAGGTTAGAACTTCTATTACACACGCAAATACATCACACATATATTACTTTCCAAACGAACTTTATACTATAGAAAAGATGATCCATCAATCATTTCCTAGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

917

Amino Acids

104.04

Weight (kDa)

6.39

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 7.1e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 98 5.9e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 147 - 207 5.7e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 156 - 239 7.4e-17 Ankyrin repeats (3 copies)
Ank_2 PF12796 214 - 277 2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 402 - 464 7.7e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 438 - 502 3.9e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 442 - 486 1.5e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 245, 2055
AatII GACGTC 1 cut(s) 1604
Acc36I ACCTGC 1 cut(s) 2603
AccB1I GGYRCC 2 cut(s) 1285, 1390
AccI GTMKAC 2 cut(s) 1120, 2062
AccII CGCG 2 cut(s) 79, 494
AciI CCGC 8 cut(s) 52, 77, 79, 161, 222, 372, 2211, 2568
AclI AACGTT 1 cut(s) 1675
AclWI GGATC 2 cut(s) 2261, 2722
AcoI YGGCCR 2 cut(s) 261, 1944
AcsI RAATTY 2 cut(s) 214, 2261
AcuI CTGAAG 1 cut(s) 2501
AcyI GRCGYC 2 cut(s) 1286, 1601
AfaI GTAC 2 cut(s) 10, 642
AfiI CCNNNNNNNGG 3 cut(s) 486, 1017, 1985
AflII CTTAAG 1 cut(s) 2014
AflIII ACRYGT 2 cut(s) 896, 2462
AjnI CCWGG 1 cut(s) 2406
AleI CACNNNNGTG 1 cut(s) 890
Alw26I GTCTC 8 cut(s) 721, 983, 1057, 1291, 1431, 1606, 2201, 2506
AlwI GGATC 2 cut(s) 2261, 2722
AoxI GGCC 4 cut(s) 224, 261, 1338, 1944
ApeKI GCWGC 5 cut(s) 476, 581, 584, 677, 1007
ApoI RAATTY 2 cut(s) 214, 2261
ArsI GACNNNNNNTTYG 2 cut(s) 2407, 2439
AspA2I CCTAGG 1 cut(s) 2744
AspLEI GCGC 3 cut(s) 345, 496, 1288
AspS9I GGNCC 4 cut(s) 224, 1339, 1853, 2410
AsuHPI GGTGA 6 cut(s) 389, 668, 863, 877, 914, 1337
AsuII TTCGAA 2 cut(s) 1524, 2259
AvaII GGWCC 2 cut(s) 1853, 2410
AvrII CCTAGG 1 cut(s) 2744
BaeGI GKGCMC 1 cut(s) 492
BalI TGGCCA 1 cut(s) 1946
BanI GGYRCC 2 cut(s) 1285, 1390
BanII GRGCYC 2 cut(s) 466, 2499
BbsI GAAGAC 2 cut(s) 741, 1695
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 5 cut(s) 488, 593, 596, 689, 1019
BccI CCATC 8 cut(s) 440, 446, 583, 647, 951, 1322, 1853, 2739
BceAI ACGGC 2 cut(s) 276, 1353
BcgI CGANNNNNNTGC 2 cut(s) 1847, 1881
BciT130I CCWGG 1 cut(s) 2408
BciVI GTATCC 2 cut(s) 1253, 1567
BclI TGATCA 3 cut(s) 865, 2131, 2596
BcoDI GTCTC 8 cut(s) 721, 983, 1057, 1291, 1431, 1606, 2201, 2506
BfaI CTAG 6 cut(s) 83, 938, 1349, 1415, 2318, 2745
BfmI CTRYAG 2 cut(s) 2106, 2715
BfoI RGCGCY 1 cut(s) 1289
BfrI CTTAAG 1 cut(s) 2014
BfuAI ACCTGC 1 cut(s) 2603
BfuI GTATCC 2 cut(s) 1253, 1567
BglII AGATCT 1 cut(s) 106
BisI GCNGC 7 cut(s) 80, 477, 582, 585, 678, 1008, 2568
BlnI CCTAGG 1 cut(s) 2744
BlsI GCNGC 7 cut(s) 81, 478, 583, 586, 679, 1009, 2569
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 2408
Bme18I GGWCC 2 cut(s) 1853, 2410
BmgT120I GGNCC 4 cut(s) 224, 1339, 1853, 2410
BmiI GGNNCC 6 cut(s) 1287, 1333, 1392, 1546, 1755, 1855
BmrFI CCNGG 1 cut(s) 2408
BmsI GCATC 1 cut(s) 2599
BpiI GAAGAC 2 cut(s) 741, 1695
BpmI CTGGAG 1 cut(s) 1226
Bpu10I CCTNAGC 1 cut(s) 681
Bpu14I TTCGAA 2 cut(s) 1524, 2259
BpuEI CTTGAG 3 cut(s) 1483, 2067, 2483
Bsa29I ATCGAT 2 cut(s) 349, 1505
BsaAI YACGTR 4 cut(s) 577, 778, 1264, 1578
BsaBI GATNNNNATC 1 cut(s) 864
BsaHI GRCGYC 2 cut(s) 1286, 1601
BsaI GGTCTC 3 cut(s) 721, 1606, 2201
BsaJI CCNNGG 7 cut(s) 77, 192, 480, 1089, 1335, 1749, 2744
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 1017, 1985
Bse1I ACTGG 1 cut(s) 1772
Bse3DI GCAATG 2 cut(s) 213, 2645
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 1 cut(s) 2408
BseCI ATCGAT 2 cut(s) 349, 1505
BseDI CCNNGG 7 cut(s) 77, 192, 480, 1089, 1335, 1749, 2744
BseGI GGATG 2 cut(s) 943, 1690
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 3 cut(s) 486, 1017, 1985
BseMI GCAATG 2 cut(s) 213, 2645
BseMII CTCAG 2 cut(s) 672, 2175
BseNI ACTGG 1 cut(s) 1772
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 5 cut(s) 488, 593, 596, 689, 1019
Bsh1236I CGCG 2 cut(s) 79, 494
BshFI GGCC 4 cut(s) 226, 263, 1340, 1946
BshNI GGYRCC 2 cut(s) 1285, 1390
BshVI ATCGAT 2 cut(s) 349, 1505
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 2162
BslI CCNNNNNNNGG 3 cut(s) 486, 1017, 1985
BsmAI GTCTC 8 cut(s) 721, 983, 1057, 1291, 1431, 1606, 2201, 2506
BsmFI GGGAC 1 cut(s) 2162
BsmI GAATGC 4 cut(s) 193, 1372, 1457, 2009
BsnI GGCC 4 cut(s) 226, 263, 1340, 1946
Bso31I GGTCTC 3 cut(s) 721, 1606, 2201
Bsp119I TTCGAA 2 cut(s) 1524, 2259
Bsp1286I GDGCHC 3 cut(s) 466, 492, 2499
Bsp143I GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
Bsp19I CCATGG 1 cut(s) 480
BspACI CCGC 8 cut(s) 52, 77, 79, 161, 222, 372, 2211, 2568
BspANI GGCC 4 cut(s) 226, 263, 1340, 1946
BspCNI CTCAG 2 cut(s) 673, 2174
BspDI ATCGAT 2 cut(s) 349, 1505
BspFNI CGCG 2 cut(s) 79, 494
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 6 cut(s) 1287, 1333, 1392, 1546, 1755, 1855
BspMI ACCTGC 1 cut(s) 2603
BspPI GGATC 2 cut(s) 2261, 2722
BspQI GCTCTTC 8 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791
BspT104I TTCGAA 2 cut(s) 1524, 2259
BspT107I GGYRCC 2 cut(s) 1285, 1390
BspTI CTTAAG 1 cut(s) 2014
BspTNI GGTCTC 3 cut(s) 721, 1606, 2201
BsrDI GCAATG 2 cut(s) 213, 2645
BsrI ACTGG 1 cut(s) 1772
BssECI CCNNGG 7 cut(s) 77, 192, 480, 1089, 1335, 1749, 2744
BssMI GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
BssNAI GTATAC 2 cut(s) 1121, 2063
BssNI GRCGYC 2 cut(s) 1286, 1601
BssT1I CCWWGG 3 cut(s) 192, 480, 2744
Bst1107I GTATAC 2 cut(s) 1121, 2063
Bst2UI CCWGG 1 cut(s) 2408
Bst4CI ACNGT 4 cut(s) 879, 1640, 2423, 2592
Bst6I CTCTTC 9 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791, 2502
BstACI GRCGYC 2 cut(s) 1286, 1601
BstAFI CTTAAG 1 cut(s) 2014
BstBAI YACGTR 4 cut(s) 577, 778, 1264, 1578
BstBI TTCGAA 2 cut(s) 1524, 2259
BstC8I GCNNGC 6 cut(s) 224, 492, 693, 1813, 2039, 2431
BstDEI CTNAG 3 cut(s) 681, 929, 2161
BstDSI CCRYGG 4 cut(s) 77, 480, 1089, 1335
BstF5I GGATG 2 cut(s) 943, 1690
BstFNI CGCG 2 cut(s) 79, 494
BstH2I RGCGCY 1 cut(s) 1289
BstHHI GCGC 3 cut(s) 345, 496, 1288
BstKTI GATC 7 cut(s) 109, 868, 1909, 2134, 2256, 2599, 2730
BstMAI GTCTC 8 cut(s) 721, 983, 1057, 1291, 1431, 1606, 2201, 2506
BstMBI GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
BstMWI GCNNNNNNNGC 5 cut(s) 203, 269, 278, 625, 962
BstNI CCWGG 1 cut(s) 2408
BstNSI RCATGY 3 cut(s) 900, 2037, 2466
BstSCI CCNGG 1 cut(s) 2406
BstSFI CTRYAG 2 cut(s) 2106, 2715
BstSLI GKGCMC 1 cut(s) 492
BstSNI TACGTA 1 cut(s) 1578
BstUI CGCG 2 cut(s) 79, 494
BstV1I GCAGC 5 cut(s) 488, 593, 596, 689, 1019
BstV2I GAAGAC 2 cut(s) 741, 1695
BstX2I RGATCY 2 cut(s) 106, 2253
BstYI RGATCY 2 cut(s) 106, 2253
BstZ17I GTATAC 2 cut(s) 1121, 2063
Bsu15I ATCGAT 2 cut(s) 349, 1505
BsuI GTATCC 2 cut(s) 1253, 1567
BsuRI GGCC 4 cut(s) 226, 263, 1340, 1946
BsuTUI ATCGAT 2 cut(s) 349, 1505
BtgI CCRYGG 4 cut(s) 77, 480, 1089, 1335
BtgZI GCGATG 2 cut(s) 602, 2284
BtsCI GGATG 2 cut(s) 943, 1690
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 430, 1141
BveI ACCTGC 1 cut(s) 2603
Cac8I GCNNGC 6 cut(s) 224, 492, 693, 1813, 2039, 2431
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 3 cut(s) 345, 496, 1288
Cfr13I GGNCC 4 cut(s) 224, 1339, 1853, 2410
Cfr42I CCGCGG 1 cut(s) 80
ClaI ATCGAT 2 cut(s) 349, 1505
Csp6I GTAC 2 cut(s) 9, 641
CviQI GTAC 2 cut(s) 9, 641
DdeI CTNAG 3 cut(s) 681, 929, 2161
DinI GGCGCC 1 cut(s) 1287
DpnI GATC 7 cut(s) 108, 867, 1908, 2133, 2255, 2598, 2729
DpnII GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
EaeI YGGCCR 2 cut(s) 261, 1944
Eam1104I CTCTTC 9 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791, 2502
EarI CTCTTC 9 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791, 2502
Eco105I TACGTA 1 cut(s) 1578
Eco130I CCWWGG 3 cut(s) 192, 480, 2744
Eco24I GRGCYC 2 cut(s) 466, 2499
Eco31I GGTCTC 3 cut(s) 721, 1606, 2201
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 1853, 2410
Eco57I CTGAAG 1 cut(s) 2501
EcoRII CCWGG 1 cut(s) 2406
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 192, 480, 2744
EcoT22I ATGCAT 2 cut(s) 1587, 2011
EcoT38I GRGCYC 2 cut(s) 466, 2499
EgeI GGCGCC 1 cut(s) 1287
EheI GGCGCC 1 cut(s) 1287
ErhI CCWWGG 3 cut(s) 192, 480, 2744
FalI AAGNNNNNCTT 2 cut(s) 2499, 2531
FaqI GGGAC 1 cut(s) 2162
FauI CCCGC 1 cut(s) 215
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 3 cut(s) 865, 2131, 2596
FblI GTMKAC 2 cut(s) 1120, 2062
Fnu4HI GCNGC 7 cut(s) 80, 477, 582, 585, 678, 1008, 2568
FokI GGATG 2 cut(s) 930, 1697
FriOI GRGCYC 2 cut(s) 466, 2499
Fsp4HI GCNGC 7 cut(s) 80, 477, 582, 585, 678, 1008, 2568
FspBI CTAG 6 cut(s) 83, 938, 1349, 1415, 2318, 2745
GlaI GCGC 3 cut(s) 344, 495, 1287
GluI GCNGC 7 cut(s) 80, 477, 582, 585, 678, 1008, 2568
GsuI CTGGAG 1 cut(s) 1226
HaeII RGCGCY 1 cut(s) 1289
HaeIII GGCC 4 cut(s) 226, 263, 1340, 1946
HapII CCGG 1 cut(s) 227
HhaI GCGC 3 cut(s) 345, 496, 1288
Hin1I GRCGYC 2 cut(s) 1286, 1601
Hin6I GCGC 3 cut(s) 343, 494, 1286
HinP1I GCGC 3 cut(s) 343, 494, 1286
HincII GTYRAC 2 cut(s) 1599, 1732
HindII GTYRAC 2 cut(s) 1599, 1732
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 4 cut(s) 970, 1805, 2120, 2214
HpaI GTTAAC 1 cut(s) 1732
HpaII CCGG 1 cut(s) 227
HphI GGTGA 6 cut(s) 389, 668, 863, 877, 914, 1337
Hpy166II GTNNAC 8 cut(s) 1121, 1599, 1732, 2063, 2089, 2204, 2539, 2618
Hpy188I TCNGA 5 cut(s) 553, 1327, 2125, 2311, 2520
Hpy188III TCNNGA 9 cut(s) 47, 1150, 1205, 1415, 1462, 2096, 2135, 2399, 2606
Hpy8I GTNNAC 8 cut(s) 1121, 1599, 1732, 2063, 2089, 2204, 2539, 2618
HpyAV CCTTC 8 cut(s) 479, 680, 1133, 1298, 1352, 1403, 1483, 1511
HpyCH4III ACNGT 4 cut(s) 879, 1640, 2423, 2592
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 269, 278, 625, 962
HpyF3I CTNAG 3 cut(s) 681, 929, 2161
Hsp92I GRCGYC 2 cut(s) 1286, 1601
HspAI GCGC 3 cut(s) 343, 494, 1286
KasI GGCGCC 1 cut(s) 1285
Ksp22I TGATCA 3 cut(s) 865, 2131, 2596
KspAI GTTAAC 1 cut(s) 1732
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
LguI GCTCTTC 8 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791
LmnI GCTCC 2 cut(s) 1759, 2589
Lsp1109I GCAGC 5 cut(s) 488, 593, 596, 689, 1019
LweI GCATC 1 cut(s) 2599
MaeI CTAG 6 cut(s) 83, 938, 1349, 1415, 2318, 2745
MaeIII GTNAC 7 cut(s) 593, 761, 820, 892, 1228, 1828, 2374
MalI GATC 7 cut(s) 108, 867, 1908, 2133, 2255, 2598, 2729
MboI GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
MflI RGATCY 2 cut(s) 106, 2253
MhlI GDGCHC 3 cut(s) 466, 492, 2499
MlsI TGGCCA 1 cut(s) 1946
MluNI TGGCCA 1 cut(s) 1946
Mly113I GGCGCC 1 cut(s) 1286
Mox20I TGGCCA 1 cut(s) 1946
Mph1103I ATGCAT 2 cut(s) 1587, 2011
MscI TGGCCA 1 cut(s) 1946
MseI TTAA 9 cut(s) 521, 747, 1316, 1449, 1658, 1731, 2015, 2418, 2646
MslI CAYNNNNRTG 4 cut(s) 600, 890, 1128, 2248
Msp20I TGGCCA 1 cut(s) 1946
MspA1I CMGCKG 2 cut(s) 79, 680
MspCI CTTAAG 1 cut(s) 2014
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 2408
Mva1269I GAATGC 4 cut(s) 193, 1372, 1457, 2009
MvaI CCWGG 1 cut(s) 2408
MvnI CGCG 2 cut(s) 79, 494
MwoI GCNNNNNNNGC 5 cut(s) 203, 269, 278, 625, 962
NarI GGCGCC 1 cut(s) 1286
NcoI CCATGG 1 cut(s) 480
NdeI CATATG 1 cut(s) 1222
NdeII GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
NlaIV GGNNCC 6 cut(s) 1287, 1333, 1392, 1546, 1755, 1855
NmeAIII GCCGAG 1 cut(s) 1730
NmuCI GTSAC 5 cut(s) 593, 820, 892, 1228, 1828
NsiI ATGCAT 2 cut(s) 1587, 2011
NspI RCATGY 3 cut(s) 900, 2037, 2466
NspV TTCGAA 2 cut(s) 1524, 2259
OliI CACNNNNGTG 1 cut(s) 890
PagI TCATGA 1 cut(s) 1149
PciI ACATGT 2 cut(s) 896, 2462
PciSI GCTCTTC 8 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791
PctI GAATGC 4 cut(s) 193, 1372, 1457, 2009
PfeI GAWTC 4 cut(s) 970, 1805, 2120, 2214
PfoI TCCNGGA 1 cut(s) 2406
PkrI GCNGC 7 cut(s) 81, 478, 583, 586, 679, 1009, 2569
PluTI GGCGCC 1 cut(s) 1289
Ppu21I YACGTR 4 cut(s) 577, 778, 1264, 1578
PscI ACATGT 2 cut(s) 896, 2462
PsiI TTATAA 2 cut(s) 245, 2055
Psp1406I AACGTT 1 cut(s) 1675
Psp6I CCWGG 1 cut(s) 2406
PspGI CCWGG 1 cut(s) 2406
PspN4I GGNNCC 6 cut(s) 1287, 1333, 1392, 1546, 1755, 1855
PspPI GGNCC 4 cut(s) 224, 1339, 1853, 2410
PsrI GAACNNNNNNTAC 2 cut(s) 2647, 2679
PsuI RGATCY 2 cut(s) 106, 2253
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 2 cut(s) 10, 642
RsaNI GTAC 2 cut(s) 9, 641
RseI CAYNNNNRTG 4 cut(s) 600, 890, 1128, 2248
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 8 cut(s) 471, 576, 672, 777, 1002, 1242, 1560, 1791
SaqAI TTAA 9 cut(s) 521, 747, 1316, 1449, 1658, 1731, 2015, 2418, 2646
SatI GCNGC 7 cut(s) 80, 477, 582, 585, 678, 1008, 2568
Sau3AI GATC 7 cut(s) 106, 865, 1906, 2131, 2253, 2596, 2727
Sau96I GGNCC 4 cut(s) 224, 1339, 1853, 2410
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 2408
SduI GDGCHC 3 cut(s) 466, 492, 2499
SfaNI GCATC 1 cut(s) 2599
SfcI CTRYAG 2 cut(s) 2106, 2715
SfoI GGCGCC 1 cut(s) 1287
Sfr303I CCGCGG 1 cut(s) 80
SfuI TTCGAA 2 cut(s) 1524, 2259
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 2 cut(s) 1853, 2410
SmiMI CAYNNNNRTG 4 cut(s) 600, 890, 1128, 2248
SmlI CTYRAG 4 cut(s) 1462, 2014, 2082, 2498
SmoI CTYRAG 4 cut(s) 1462, 2014, 2082, 2498
SnaBI TACGTA 1 cut(s) 1578
SsiI CCGC 8 cut(s) 52, 77, 79, 161, 222, 372, 2211, 2568
SspDI GGCGCC 1 cut(s) 1285
SspI AATATT 1 cut(s) 847
SspMI CTAG 6 cut(s) 83, 938, 1349, 1415, 2318, 2745
StyD4I CCNGG 1 cut(s) 2406
StyI CCWWGG 3 cut(s) 192, 480, 2744
TaaI ACNGT 4 cut(s) 879, 1640, 2423, 2592
TaqI TCGA 5 cut(s) 349, 1505, 1524, 2240, 2259
TatI WGTACW 2 cut(s) 8, 640
TauI GCSGC 2 cut(s) 82, 2570
TfiI GAWTC 4 cut(s) 970, 1805, 2120, 2214
Tru1I TTAA 9 cut(s) 521, 747, 1316, 1449, 1658, 1731, 2015, 2418, 2646
Tru9I TTAA 9 cut(s) 521, 747, 1316, 1449, 1658, 1731, 2015, 2418, 2646
TscAI CASTG 2 cut(s) 430, 1141
TseFI GTSAC 5 cut(s) 593, 820, 892, 1228, 1828
TseI GCWGC 5 cut(s) 476, 581, 584, 677, 1007
Tsp45I GTSAC 5 cut(s) 593, 820, 892, 1228, 1828
TspGWI ACGGA 4 cut(s) 1106, 1816, 1866, 2189
TspRI CASTG 2 cut(s) 430, 1141
Vha464I CTTAAG 1 cut(s) 2014
VpaK11BI GGWCC 2 cut(s) 1853, 2410
XapI RAATTY 2 cut(s) 214, 2261
XbaI TCTAGA 1 cut(s) 1414
XceI RCATGY 3 cut(s) 900, 2037, 2466
XmaJI CCTAGG 1 cut(s) 2744
XmiI GTMKAC 2 cut(s) 1120, 2062
XspI CTAG 6 cut(s) 83, 938, 1349, 1415, 2318, 2745
ZraI GACGTC 1 cut(s) 1602
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 2 cut(s) 1587, 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.