Rh7BG443400

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000952
Physical Location & Seq
Forward (+)
51268641 .. 51283766
15126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG443400.1

Sequence Viewer

Length: 2100 bp
ATGGCAAATACTCCAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTGAAAACTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCGAAGCAATCGTGAAACCTCCAAAACGTGAAGATGATCAAACTGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTGTACGCAATCCAAAATACATGCGGGAGAGAGCTGTTCTCTTTTATAAGGGAAATTGAGACGGCCAAAAGTGCCTGGGAGGCTTTGGAACAAGAGTGCAAAGTTATGCAAGGATATGATGAGAACAATGCCAACCACTCAGTCCGCTATAAATTGTTCATAGAATCAGTGAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATGCGCAGTGTAGTAGTAAGGGCAGTAGATGGAGATGGAGAAATCGCTCTTCATGTAGCAGCCAAGGAAGGGCATGCGCACATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTGAAAACGAAAACCGCTCATGGTCACACAGCTCTTCACCTAGCAGCAGCGGAGGGGCATGTGCATGTTGTGAAAGAGTTGCCGGTGCTGTTGATGGGAGAAGTACAAGACTGGGAAGGTGACACAGCTCTTCATATAGCAGTCCAGGAAGGGAAGGTCGATGTTGTTAAAGAGTTGGTGCTGTTGATGAGACAAGAAGACTTGAAAATAAAAAATGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAATGTGCCTATGGTCAAAGAGTTCGTGAGAAAAGAAAAAGGTGAGAATAATATTGGCGAGGATGACTTTGAGCGTTATATACCGTTTACCACCTATGTGCAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTGGATAATCCATGTGGTGCAGTAACAGCAGTAGATCCAAGAGACTACCACCGAGACACTGCTCTTCACGTAGCAGCCCGAAGAGGGCATGTGGATGTTACCAAAGGATTGTTGTTGTTGCTGAAAATGAGACGAGAAGATTTGGAATTGAAAACCGCTGAAAATTTCACAACTTCTGGTTCTAGTATAACTTTAGGGGTCAGTGAAGAATTTCTCATGGAAAGGGCCAAGTACATGGTTGAACAGTATGAGAAAACACTTGGCAGCTGTTTGGAGATACAAAACGCTAGAGGTGTCACAGCTCTCCACATGGCAGTACTGAACAGGCGTGTGGATATTGTGAAAGAGTTGGTGCCATTGATGAGAAAAGAAGGTTTAGAAATAAAAGACGTTGACGGAGACACAGCTCTCCACGTGGTAGTACGGAACGGGCGTGTGGATATTGTGAAAGAGTTGGTGCCATTGATGAGAAAAGAAGGTTTAAAAATAAAAGACGTTGACGGAGACACAGCTCTCCACGTGGCAGTACGGAACGGGCGTGTGGATATTGTGAAAGAGTTGGTGCCATTAATGAGAAAAGAAGATTTTGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCTATCTTACAAAGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACCAGAGGCTTTCGAAGAAAACGACGGTGAAGGTTGTACAGCTTTAAATATGGCTATTAAATGTTTCAAGGATGGAGACGTTTGCGAAATGGCTAAATACATGGCTGAAAAGAATGTTAGAGTACTTGGCATACCCTCGGCTCCCGATGATGAGATTCCAGTTGTCGAAGCTTGTCGTTGGAGTAAATGGAAATCGGCTCGCTATATGTATTCTGTCACTCCACATGAAGCTCTAGAGGGACCCACTGGCAGTTTACTTATTTGCTGTTGTCTCAATTCTAGCAAAACGATTGATTTGGCATGGGACTTACTTCAACATTGCCCAAATTTGGCCTTCACTATCACTACACCGCCTAAAACCACACCTATGTTGAAATTGGCTCGTATGCGTTTTGCATTCTTAAGTGGAACACAACTCACACGCTGGCAAAGGTGGCTTTATGATCGTGAGTACAAAACACTTCATTTTCTTTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

699

Amino Acids

78.94

Weight (kDa)

5.69

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2.4e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 2.6e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 147 - 207 6.5e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 149 - 195 8.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 157 - 240 8.1e-15 Ankyrin repeats (3 copies)
Ank_5 PF13857 215 - 262 9.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 224 - 351 2.3e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 406 - 451 6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 415 - 511 1.5e-16 Ankyrin repeats (3 copies)
Ank_5 PF13857 481 - 523 3.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 506 - 579 1.3e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 248
Acc16I TGCGCA 2 cut(s) 425, 498
AccB1I GGYRCC 3 cut(s) 1291, 1396, 1501
AccBSI CCGCTC 1 cut(s) 557
AccII CGCG 1 cut(s) 79
AciI CCGC 6 cut(s) 225, 346, 555, 590, 1095, 1970
AclWI GGATC 1 cut(s) 968
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 3 cut(s) 1102, 1148, 1945
AcuI CTGAAG 1 cut(s) 123
AcvI CACGTG 2 cut(s) 1354, 1459
AdeI CACNNNGTG 1 cut(s) 505
AfaI GTAC 9 cut(s) 206, 645, 1172, 1257, 1362, 1467, 1657, 1743, 2072
AfiI CCNNNNNNNGG 3 cut(s) 489, 1023, 1948
AflII CTTAAG 1 cut(s) 2020
AgsI TTSAA 9 cut(s) 85, 544, 745, 1090, 1181, 1529, 1687, 1934, 1993
AjnI CCWGG 3 cut(s) 176, 275, 684
AleI CACNNNNGTG 1 cut(s) 893
AlwI GGATC 1 cut(s) 968
AoxI GGCC 4 cut(s) 174, 264, 1164, 1950
ApeKI GCWGC 5 cut(s) 479, 584, 587, 1013, 1203
ApoI RAATTY 3 cut(s) 1102, 1148, 1945
AseI ATTAAT 1 cut(s) 1508
Asp700I GAANNNNTTC 1 cut(s) 1149
AspLEI GCGC 2 cut(s) 426, 499
AspS9I GGNCC 3 cut(s) 180, 1164, 1859
AsuHPI GGTGA 7 cut(s) 392, 569, 671, 770, 851, 917, 1658
AsuII TTCGAA 1 cut(s) 1632
AvaII GGWCC 2 cut(s) 180, 1859
BanI GGYRCC 3 cut(s) 1291, 1396, 1501
BbrPI CACGTG 2 cut(s) 1354, 1459
BbsI GAAGAC 1 cut(s) 744
BbvI GCAGC 5 cut(s) 491, 596, 599, 1025, 1215
BccI CCATC 5 cut(s) 443, 449, 628, 1538, 1685
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 3 cut(s) 178, 277, 686
BclI TGATCA 1 cut(s) 157
BfaI CTAG 6 cut(s) 581, 782, 1122, 1227, 1853, 1899
BfrI CTTAAG 1 cut(s) 2020
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 480, 585, 588, 1014, 1204
BlsI GCNGC 5 cut(s) 481, 586, 589, 1015, 1205
BmcAI AGTACT 2 cut(s) 1257, 1743
Bme1390I CCNGG 3 cut(s) 178, 277, 686
Bme18I GGWCC 2 cut(s) 180, 1859
BmgT120I GGNCC 3 cut(s) 180, 1164, 1859
BmiI GGNNCC 6 cut(s) 1293, 1398, 1503, 1761, 1860, 1861
BmrFI CCNGG 3 cut(s) 178, 277, 686
BmrI ACTGGG 1 cut(s) 661
BmsI GCATC 1 cut(s) 748
BmuI ACTGGG 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 744
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu14I TTCGAA 1 cut(s) 1632
Bsa29I ATCGAT 1 cut(s) 1613
BsaAI YACGTR 3 cut(s) 1009, 1354, 1459
BsaI GGTCTC 1 cut(s) 1612
BsaJI CCNNGG 4 cut(s) 195, 276, 483, 1755
Bsc4I CCNNNNNNNGG 3 cut(s) 489, 1023, 1948
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 5 cut(s) 14, 656, 945, 1778, 1870
Bse3DI GCAATG 2 cut(s) 1563, 1936
BseBI CCWGG 3 cut(s) 178, 277, 686
BseCI ATCGAT 1 cut(s) 1613
BseDI CCNNGG 4 cut(s) 195, 276, 483, 1755
BseGI GGATG 3 cut(s) 865, 1039, 1696
BseLI CCNNNNNNNGG 3 cut(s) 489, 1023, 1948
BseMI GCAATG 2 cut(s) 1563, 1936
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 5 cut(s) 14, 656, 945, 1778, 1870
BseXI GCAGC 5 cut(s) 491, 596, 599, 1025, 1215
BsgI GTGCAG 1 cut(s) 978
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 4 cut(s) 176, 266, 1166, 1952
BshNI GGYRCC 3 cut(s) 1291, 1396, 1501
BshVI ATCGAT 1 cut(s) 1613
BsiSI CCGG 1 cut(s) 623
BslFI GGGAC 2 cut(s) 1872, 1937
BslI CCNNNNNNNGG 3 cut(s) 489, 1023, 1948
BsmBI CGTCTC 3 cut(s) 254, 1063, 1689
BsmFI GGGAC 2 cut(s) 1872, 1937
BsmI GAATGC 2 cut(s) 196, 2015
BsnI GGCC 4 cut(s) 176, 266, 1166, 1952
Bso31I GGTCTC 1 cut(s) 1612
Bsp119I TTCGAA 1 cut(s) 1632
Bsp1407I TGTACA 1 cut(s) 1655
Bsp143I GATC 5 cut(s) 74, 106, 157, 973, 2062
Bsp68I TCGCGA 1 cut(s) 79
BspACI CCGC 6 cut(s) 225, 346, 555, 590, 1095, 1970
BspANI GGCC 4 cut(s) 176, 266, 1166, 1952
BspCNI CTCAG 1 cut(s) 353
BspDI ATCGAT 1 cut(s) 1613
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 6 cut(s) 1293, 1398, 1503, 1761, 1860, 1861
BspPI GGATC 1 cut(s) 968
BspQI GCTCTTC 5 cut(s) 474, 579, 675, 780, 1008
BspT104I TTCGAA 1 cut(s) 1632
BspT107I GGYRCC 3 cut(s) 1291, 1396, 1501
BspTI CTTAAG 1 cut(s) 2020
BspTNI GGTCTC 1 cut(s) 1612
BsrBI CCGCTC 1 cut(s) 557
BsrDI GCAATG 2 cut(s) 1563, 1936
BsrFI RCCGGY 1 cut(s) 622
BsrGI TGTACA 1 cut(s) 1655
BsrI ACTGG 5 cut(s) 14, 656, 945, 1778, 1870
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 4 cut(s) 195, 276, 483, 1755
BssMI GATC 5 cut(s) 74, 106, 157, 973, 2062
BssT1I CCWWGG 2 cut(s) 195, 483
Bst2UI CCWGG 3 cut(s) 178, 277, 686
Bst4CI ACNGT 4 cut(s) 53, 882, 1185, 1646
Bst6I CTCTTC 6 cut(s) 474, 579, 675, 780, 1008, 1015
BstAFI CTTAAG 1 cut(s) 2020
BstAUI TGTACA 1 cut(s) 1655
BstBAI YACGTR 3 cut(s) 1009, 1354, 1459
BstBI TTCGAA 1 cut(s) 1632
BstC8I GCNNGC 3 cut(s) 495, 1819, 2045
BstDEI CTNAG 2 cut(s) 340, 932
BstF5I GGATG 3 cut(s) 865, 1039, 1696
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 2 cut(s) 426, 499
BstKTI GATC 5 cut(s) 77, 109, 160, 976, 2065
BstMBI GATC 5 cut(s) 74, 106, 157, 973, 2062
BstMWI GCNNNNNNNGC 6 cut(s) 125, 206, 272, 281, 965, 2053
BstNI CCWGG 3 cut(s) 178, 277, 686
BstNSI RCATGY 6 cut(s) 225, 497, 602, 608, 1031, 1586
BstSCI CCNGG 3 cut(s) 176, 275, 684
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 5 cut(s) 491, 596, 599, 1025, 1215
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 2 cut(s) 106, 973
BstXI CCANNNNNNTGG 1 cut(s) 1174
BstYI RGATCY 2 cut(s) 106, 973
Bsu15I ATCGAT 1 cut(s) 1613
BsuRI GGCC 4 cut(s) 176, 266, 1166, 1952
BsuTUI ATCGAT 1 cut(s) 1613
BtsCI GGATG 3 cut(s) 865, 1039, 1696
BtsI GCAGTG 3 cut(s) 382, 433, 996
BtsIMutI CAGTG 6 cut(s) 375, 382, 433, 996, 1147, 1863
BtuMI TCGCGA 1 cut(s) 79
Cac8I GCNNGC 3 cut(s) 495, 1819, 2045
CfoI GCGC 2 cut(s) 426, 499
Cfr10I RCCGGY 1 cut(s) 622
Cfr13I GGNCC 3 cut(s) 180, 1164, 1859
ClaI ATCGAT 1 cut(s) 1613
CseI GACGC 1 cut(s) 1547
Csp6I GTAC 9 cut(s) 205, 644, 1171, 1256, 1361, 1466, 1656, 1742, 2071
CviQI GTAC 9 cut(s) 205, 644, 1171, 1256, 1361, 1466, 1656, 1742, 2071
DdeI CTNAG 2 cut(s) 340, 932
DpnI GATC 5 cut(s) 76, 108, 159, 975, 2064
DpnII GATC 5 cut(s) 74, 106, 157, 973, 2062
DraI TTTAAA 2 cut(s) 1422, 1665
DraIII CACNNNGTG 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 6 cut(s) 474, 579, 675, 780, 1008, 1015
EarI CTCTTC 6 cut(s) 474, 579, 675, 780, 1008, 1015
Eco130I CCWWGG 2 cut(s) 195, 483
Eco147I AGGCCT 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 1612
Eco47I GGWCC 2 cut(s) 180, 1859
Eco57I CTGAAG 1 cut(s) 123
Eco72I CACGTG 2 cut(s) 1354, 1459
EcoO109I RGGNCCY 1 cut(s) 1859
EcoRII CCWGG 3 cut(s) 176, 275, 684
EcoT14I CCWWGG 2 cut(s) 195, 483
ErhI CCWWGG 2 cut(s) 195, 483
Esp3I CGTCTC 3 cut(s) 254, 1063, 1689
FaqI GGGAC 2 cut(s) 1872, 1937
FauI CCCGC 1 cut(s) 218
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 480, 585, 588, 1014, 1204
FokI GGATG 3 cut(s) 872, 1046, 1703
Fsp4HI GCNGC 5 cut(s) 480, 585, 588, 1014, 1204
FspAI RTGCGCAY 1 cut(s) 498
FspBI CTAG 6 cut(s) 581, 782, 1122, 1227, 1853, 1899
FspI TGCGCA 2 cut(s) 425, 498
GlaI GCGC 2 cut(s) 425, 498
GluI GCNGC 5 cut(s) 480, 585, 588, 1014, 1204
HaeIII GGCC 4 cut(s) 176, 266, 1166, 1952
HapII CCGG 1 cut(s) 623
HgaI GACGC 1 cut(s) 1547
HhaI GCGC 2 cut(s) 426, 499
Hin6I GCGC 2 cut(s) 424, 497
HinP1I GCGC 2 cut(s) 424, 497
HincII GTYRAC 2 cut(s) 1333, 1438
HindII GTYRAC 2 cut(s) 1333, 1438
HindIII AAGCTT 1 cut(s) 1788
HinfI GANTC 2 cut(s) 365, 1774
HpaII CCGG 1 cut(s) 623
HphI GGTGA 7 cut(s) 392, 569, 671, 770, 851, 917, 1658
Hpy166II GTNNAC 4 cut(s) 885, 1333, 1438, 1874
Hpy188III TCNNGA 9 cut(s) 38, 47, 78, 133, 505, 823, 1763, 1853, 2066
Hpy8I GTNNAC 4 cut(s) 885, 1333, 1438, 1874
Hpy99I CGWCG 1 cut(s) 1646
HpyAV CCTTC 9 cut(s) 482, 650, 683, 688, 1304, 1409, 1591, 1643, 1963
HpyCH4III ACNGT 4 cut(s) 53, 882, 1185, 1646
HpyCH4IV ACGT 7 cut(s) 148, 1008, 1329, 1353, 1434, 1458, 1698
HpyCH4V TGCA 9 cut(s) 29, 300, 310, 520, 604, 898, 959, 1609, 2015
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 206, 272, 281, 965, 2053
HpyF3I CTNAG 2 cut(s) 340, 932
HpySE526I ACGT 7 cut(s) 148, 1008, 1329, 1353, 1434, 1458, 1698
HspAI GCGC 2 cut(s) 424, 497
KflI GGGWCCC 1 cut(s) 1859
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 74, 106, 157, 973, 2062
LguI GCTCTTC 5 cut(s) 474, 579, 675, 780, 1008
LmnI GCTCC 1 cut(s) 1765
Lsp1109I GCAGC 5 cut(s) 491, 596, 599, 1025, 1215
LweI GCATC 1 cut(s) 748
MaeI CTAG 6 cut(s) 581, 782, 1122, 1227, 1853, 1899
MaeII ACGT 7 cut(s) 148, 1008, 1329, 1353, 1434, 1458, 1698
MaeIII GTNAC 8 cut(s) 56, 563, 659, 764, 961, 1036, 1234, 1834
MalI GATC 5 cut(s) 76, 108, 159, 975, 2064
MbiI CCGCTC 1 cut(s) 557
MboI GATC 5 cut(s) 74, 106, 157, 973, 2062
MflI RGATCY 2 cut(s) 106, 973
MluCI AATT 8 cut(s) 255, 353, 1085, 1102, 1148, 1894, 1945, 1994
MmeI TCCRAC 1 cut(s) 1778
MroXI GAANNNNTTC 1 cut(s) 1149
MseI TTAA 8 cut(s) 708, 1421, 1508, 1560, 1664, 1677, 2021, 2098
MslI CAYNNNNRTG 4 cut(s) 603, 893, 1032, 1985
MspA1I CMGCKG 3 cut(s) 590, 1097, 1206
MspCI CTTAAG 1 cut(s) 2020
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 3 cut(s) 178, 277, 686
Mva1269I GAATGC 2 cut(s) 196, 2015
MvaI CCWGG 3 cut(s) 178, 277, 686
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 6 cut(s) 125, 206, 272, 281, 965, 2053
NdeII GATC 5 cut(s) 74, 106, 157, 973, 2062
NlaIV GGNNCC 6 cut(s) 1293, 1398, 1503, 1761, 1860, 1861
NmeAIII GCCGAG 1 cut(s) 1736
NmuCI GTSAC 4 cut(s) 563, 659, 1234, 1834
NruI TCGCGA 1 cut(s) 79
NsbI TGCGCA 2 cut(s) 425, 498
NspI RCATGY 6 cut(s) 225, 497, 602, 608, 1031, 1586
NspV TTCGAA 1 cut(s) 1632
OliI CACNNNNGTG 1 cut(s) 893
PaeI GCATGC 1 cut(s) 497
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 5 cut(s) 474, 579, 675, 780, 1008
PcsI WCGNNNNNNNCGW 4 cut(s) 129, 1369, 1474, 1638
PctI GAATGC 2 cut(s) 196, 2015
PdmI GAANNNNTTC 1 cut(s) 1149
PfeI GAWTC 2 cut(s) 365, 1774
PfoI TCCNGGA 1 cut(s) 684
PkrI GCNGC 5 cut(s) 481, 586, 589, 1015, 1205
PmaCI CACGTG 2 cut(s) 1354, 1459
PmlI CACGTG 2 cut(s) 1354, 1459
Ppu21I YACGTR 3 cut(s) 1009, 1354, 1459
PpuMI RGGWCCY 1 cut(s) 1859
PshBI ATTAAT 1 cut(s) 1508
PsiI TTATAA 2 cut(s) 171, 248
Psp5II RGGWCCY 1 cut(s) 1859
Psp6I CCWGG 3 cut(s) 176, 275, 684
PspCI CACGTG 2 cut(s) 1354, 1459
PspGI CCWGG 3 cut(s) 176, 275, 684
PspN4I GGNNCC 6 cut(s) 1293, 1398, 1503, 1761, 1860, 1861
PspPI GGNCC 3 cut(s) 180, 1164, 1859
PspPPI RGGWCCY 1 cut(s) 1859
PsuI RGATCY 2 cut(s) 106, 973
PvuII CAGCTG 1 cut(s) 1206
RruI TCGCGA 1 cut(s) 79
RsaI GTAC 9 cut(s) 206, 645, 1172, 1257, 1362, 1467, 1657, 1743, 2072
RsaNI GTAC 9 cut(s) 205, 644, 1171, 1256, 1361, 1466, 1656, 1742, 2071
RseI CAYNNNNRTG 4 cut(s) 603, 893, 1032, 1985
SapI GCTCTTC 5 cut(s) 474, 579, 675, 780, 1008
SaqAI TTAA 8 cut(s) 708, 1421, 1508, 1560, 1664, 1677, 2021, 2098
SatI GCNGC 5 cut(s) 480, 585, 588, 1014, 1204
Sau3AI GATC 5 cut(s) 74, 106, 157, 973, 2062
Sau96I GGNCC 3 cut(s) 180, 1164, 1859
ScaI AGTACT 2 cut(s) 1257, 1743
ScrFI CCNGG 3 cut(s) 178, 277, 686
SfaNI GCATC 1 cut(s) 748
SfuI TTCGAA 1 cut(s) 1632
SinI GGWCC 2 cut(s) 180, 1859
SmiMI CAYNNNNRTG 4 cut(s) 603, 893, 1032, 1985
SmlI CTYRAG 1 cut(s) 2020
SmoI CTYRAG 1 cut(s) 2020
SphI GCATGC 1 cut(s) 497
Sse9I AATT 8 cut(s) 255, 353, 1085, 1102, 1148, 1894, 1945, 1994
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 6 cut(s) 225, 346, 555, 590, 1095, 1970
SspI AATATT 1 cut(s) 850
SspMI CTAG 6 cut(s) 581, 782, 1122, 1227, 1853, 1899
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 176, 275, 684
StyI CCWWGG 2 cut(s) 195, 483
TaaI ACNGT 4 cut(s) 53, 882, 1185, 1646
TaiI ACGT 7 cut(s) 151, 1011, 1332, 1356, 1437, 1461, 1701
TaqI TCGA 5 cut(s) 123, 699, 1613, 1632, 1785
TasI AATT 8 cut(s) 255, 353, 1085, 1102, 1148, 1894, 1945, 1994
TatI WGTACW 6 cut(s) 643, 1170, 1255, 1655, 1741, 2070
TfiI GAWTC 2 cut(s) 365, 1774
Tru1I TTAA 8 cut(s) 708, 1421, 1508, 1560, 1664, 1677, 2021, 2098
Tru9I TTAA 8 cut(s) 708, 1421, 1508, 1560, 1664, 1677, 2021, 2098
TscAI CASTG 6 cut(s) 375, 382, 433, 1003, 1147, 1870
TseFI GTSAC 4 cut(s) 563, 659, 1234, 1834
TseI GCWGC 5 cut(s) 479, 584, 587, 1013, 1203
Tsp45I GTSAC 4 cut(s) 563, 659, 1234, 1834
TspDTI ATGAA 6 cut(s) 78, 349, 461, 662, 1860, 2072
TspGWI ACGGA 4 cut(s) 1350, 1378, 1455, 1483
TspRI CASTG 6 cut(s) 375, 382, 433, 1003, 1147, 1870
Vha464I CTTAAG 1 cut(s) 2020
VpaK11BI GGWCC 2 cut(s) 180, 1859
VspI ATTAAT 1 cut(s) 1508
XapI RAATTY 3 cut(s) 1102, 1148, 1945
XbaI TCTAGA 1 cut(s) 1852
XceI RCATGY 6 cut(s) 225, 497, 602, 608, 1031, 1586
XmnI GAANNNNTTC 1 cut(s) 1149
XspI CTAG 6 cut(s) 581, 782, 1122, 1227, 1853, 1899
ZrmI AGTACT 2 cut(s) 1257, 1743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.