Rh7BG443700

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000952
Physical Location & Seq
Forward (+)
51470623 .. 51486555
15933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG443700.1

Sequence Viewer

Length: 2118 bp
ATGGACCAAGGTCCACCTGCACCAAGCCACTACGTGAGGCACGATTCGAAGCCTCTTCCATTAAACCGGCCACCTGCAGAACAGAGACAACCAGTCGGCGTCGGTGTCTTCGCCTCCATCTCCGCTGACGGCTCCGTCAGAGTGTTCGACCTGCCTGACAAGGAGCATTCCGCGATGATCTACGAGAGTTCCGATCCCGACACCCCCTTGGTCCGCCTCGGATGGAGCAAGCAGGACTCCAGGTACATGGCCACCACCATCATGGATAGCGCCAAGGCCGTCGTCCTCGACATCCGCTTTCCTGCTCATCCGGTCGTCGAGTTGCAGAGGACGACTGCACTGCCGGGGACGACGTTGCCGCCGGAAAGATCGGTGAAGCCGAGATTGATAGCTAGCATGGAAAGTATTCAAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTAACCGCGATAACTATAAAAAGTGGAGGTTACGGGTAAAAACTTATTTGTTGTCTGAAGATCTATGGGATGTTGTCGAAGCAATCACGGAACCTGCAAAACTAGAAGATGATCAAGCCGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTATACGCAATTCAAAATTCATGCGGACTAGAGATGTTTTCTTTTATAAGCGAAACCGAATCGGCGAAAATGGCGTGGGAGGCTTTGGAACAAGAGTGCAAAGTTGTACAAGGATATAATGAGAACAATGCCAACCACTTGATGTGCTATAAATCGTTCATTGAGTCGGTAAGCAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATCCGCAGTGTAGTAGTAGTAAAAGCGGTAGGCACATATGGATATATAGCTCTTCATGTAGCAGCCTGGGAAGGGCACGTGCATGTAGTGAAAGAGCTGGTGCCTTTCATGACACAAGAAGATTTTAAAACAAAAAACGATTTTGGTCACACAGCTCTTCATCTAGCAGCAGAGAAGGGGCATGTACATATTGTGAAAGAGTTGCCGGTGCCGTTGATGGGAGAAGTAAAAGACAAAGATGGTGACACGGCTCTTCATATAGCAGTCCAGGAAGGGCAGGCCGATGTTGTGAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACTTGAAAATTAAAAACGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAACGTGCCTATGGTCAAACAGTTCGTTACAAAAGAAAAAGGTGAGAATAATATTGGCAAGGACGACTTTGAGCGTTATATACCGTTTACCACTTATGTGGAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCATGTGGTGCAATAACAGCAGTAGATCCAAGAGACGACTACCGAGATACTGCTCTTCACGTAGCAGCCCGGCAAGGGTATTTGGACGTTACTAGAGGATTGGTGTTGTTACTGAAAGTGAGAAAAGAAGATTTGGAATTGAAAACCGCTGAAGATTTCACAGCTTTTGGTTCTGGTATAACTTTAGGGGTCAGTGAAGAATTCCTCATGGAAAGGGCCAAATACATGGTTGAACAGTACGAGAAAACACTTGGCAGCCTTTTGGAGATACAAAACGCTGAAGGGTCCACAGCTCTTCACCTAGCAGTACTGCAGGAGTATGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGTTTAGAAATAAAAGACGCTGATGGAAACACAGCTCTTAACATTGCAATCTTACAAGGGCATGTGGATATTGCGAAAGAGTTGGTGCAATCGATGAGACCAGAGGCTTTGGAAGAAAAAGACGGTCAAGGTTATACAGCTTTAAGGATGGCTATTAAATATTTCGAGGATGGAGACATTTGCGAAATGGCTAAATGCGTGGCTGAAAAGAATAAAAAAGTATTTGGCATACCCTTTGATGATGACATTCCAGTTGTCACAGTTGTTCGTTCGGGTGAATGGAAATCGGCTCGCTATATGTATTCTGTCACTCCACTTGAAGCTCTCGAGGGACCCAACGGCAGTTCACTTATTTGCTTATGTCTTTGTGCTAGCAACACGATTGGTAAGGCTACTTTCAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

705

Amino Acids

78.45

Weight (kDa)

5.19

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 148 - 174 5e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 161 - 233 1.3e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 290 - 373 1.6e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 348 - 413 2.2e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 350 - 395 6.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 377 - 484 1.6e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 548 - 632 1e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 576 - 621 4.9e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 567, 644
AarI CACCTGC 2 cut(s) 25, 82
AasI GACNNNNNNGTC 1 cut(s) 134
Acc36I ACCTGC 4 cut(s) 25, 82, 159, 544
AccB1I GGYRCC 3 cut(s) 916, 1024, 1690
AccII CGCG 2 cut(s) 173, 450
AclWI GGATC 2 cut(s) 188, 1367
AcoI YGGCCR 2 cut(s) 68, 249
AcsI RAATTY 2 cut(s) 613, 1547
AcuI CTGAAG 3 cut(s) 519, 1518, 1647
AcvI CACGTG 1 cut(s) 895
AcyI GRCGYC 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 34
AfaI GTAC 5 cut(s) 245, 705, 1002, 1586, 1656
AfiI CCNNNNNNNGG 3 cut(s) 888, 1034, 1422
AgsI TTSAA 6 cut(s) 410, 611, 1144, 1489, 1580, 2030
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 4 cut(s) 239, 572, 881, 1083
AleI CACNNNNGTG 1 cut(s) 1292
Alw26I GTCTC 5 cut(s) 79, 1123, 1374, 1801, 1878
AlwI GGATC 2 cut(s) 188, 1367
Ama87I CYCGRG 1 cut(s) 2036
AoxI GGCC 6 cut(s) 68, 249, 276, 570, 1095, 1563
ApeKI GCWGC 4 cut(s) 878, 983, 1412, 1602
ApoI RAATTY 2 cut(s) 613, 1547
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 7 cut(s) 4, 11, 211, 576, 1563, 1632, 2042
AsuC2I CCSGG 2 cut(s) 345, 1417
AsuHPI GGTGA 8 cut(s) 385, 788, 1070, 1169, 1250, 1316, 1637, 1997
AsuII TTCGAA 1 cut(s) 47
AsuNHI GCTAGC 2 cut(s) 392, 2081
AvaI CYCGRG 1 cut(s) 2036
AvaII GGWCC 6 cut(s) 4, 11, 211, 576, 1632, 2042
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 3 cut(s) 916, 1024, 1690
BbrPI CACGTG 1 cut(s) 895
BbsI GAAGAC 2 cut(s) 100, 1143
BbvI GCAGC 4 cut(s) 890, 995, 1424, 1614
BccI CCATC 8 cut(s) 125, 216, 266, 1027, 1049, 1727, 1852, 1874
BceAI ACGGC 5 cut(s) 145, 263, 1012, 1080, 2065
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 4 cut(s) 241, 574, 883, 1085
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 2 cut(s) 345, 1417
BcoDI GTCTC 5 cut(s) 79, 1123, 1374, 1801, 1878
BfaI CTAG 9 cut(s) 393, 545, 626, 980, 1181, 1340, 1440, 1649, 2082
BfmI CTRYAG 2 cut(s) 75, 1658
BfoI RGCGCY 1 cut(s) 273
BfuAI ACCTGC 4 cut(s) 25, 82, 159, 544
BglII AGATCT 1 cut(s) 502
BisI GCNGC 5 cut(s) 359, 879, 984, 1413, 1603
BlsI GCNGC 5 cut(s) 360, 880, 985, 1414, 1604
BmcAI AGTACT 1 cut(s) 1656
Bme1390I CCNGG 6 cut(s) 241, 345, 574, 883, 1085, 1417
Bme18I GGWCC 6 cut(s) 4, 11, 211, 576, 1632, 2042
BmeRI GACNNNNNGTC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 2036
BmgT120I GGNCC 7 cut(s) 4, 11, 211, 576, 1563, 1632, 2042
BmiI GGNNCC 8 cut(s) 133, 534, 918, 1026, 1633, 1692, 2043, 2044
BmrFI CCNGG 6 cut(s) 241, 345, 574, 883, 1085, 1417
BmsI GCATC 1 cut(s) 1147
BmtI GCTAGC 2 cut(s) 396, 2085
BoxI GACNNNNGTC 1 cut(s) 9
BpiI GAAGAC 2 cut(s) 100, 1143
BpmI CTGGAG 1 cut(s) 223
Bpu14I TTCGAA 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 345, 1417
Bsa29I ATCGAT 1 cut(s) 1802
BsaAI YACGTR 3 cut(s) 34, 895, 1408
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 1801
BsaJI CCNNGG 7 cut(s) 7, 207, 217, 273, 344, 591, 882
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 3 cut(s) 888, 1034, 1422
Bse118I RCCGGY 2 cut(s) 66, 1021
Bse1I ACTGG 3 cut(s) 92, 1694, 1961
Bse3DI GCAATG 1 cut(s) 1752
BseBI CCWGG 4 cut(s) 241, 574, 883, 1085
BseCI ATCGAT 1 cut(s) 1802
BseDI CCNNGG 7 cut(s) 7, 207, 217, 273, 344, 591, 882
BseGI GGATG 7 cut(s) 227, 291, 307, 517, 1678, 1863, 1885
BseLI CCNNNNNNNGG 3 cut(s) 888, 1034, 1422
BseMI GCAATG 1 cut(s) 1752
BseNI ACTGG 3 cut(s) 92, 1694, 1961
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 4 cut(s) 890, 995, 1424, 1614
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 2 cut(s) 173, 450
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 6 cut(s) 70, 251, 278, 572, 1097, 1565
BshNI GGYRCC 3 cut(s) 916, 1024, 1690
BshVI ATCGAT 1 cut(s) 1802
BsiEI CGRYCG 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 2036
BsiSI CCGG 6 cut(s) 67, 311, 344, 362, 1022, 1417
BslFI GGGAC 2 cut(s) 361, 2055
BslI CCNNNNNNNGG 3 cut(s) 888, 1034, 1422
BsmAI GTCTC 5 cut(s) 79, 1123, 1374, 1801, 1878
BsmBI CGTCTC 1 cut(s) 1374
BsmFI GGGAC 2 cut(s) 361, 2055
BsmI GAATGC 2 cut(s) 166, 592
BsnI GGCC 6 cut(s) 70, 251, 278, 572, 1097, 1565
Bso31I GGTCTC 1 cut(s) 1801
BsoBI CYCGRG 1 cut(s) 2036
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 894
Bsp1407I TGTACA 2 cut(s) 703, 1000
Bsp143I GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
BspANI GGCC 6 cut(s) 70, 251, 278, 572, 1097, 1565
BspDI ATCGAT 1 cut(s) 1802
BspFNI CGCG 2 cut(s) 173, 450
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 8 cut(s) 133, 534, 918, 1026, 1633, 1692, 2043, 2044
BspMAI CTGCAG 2 cut(s) 79, 1662
BspMI ACCTGC 4 cut(s) 25, 82, 159, 544
BspOI GCTAGC 2 cut(s) 396, 2085
BspPI GGATC 2 cut(s) 188, 1367
BspQI GCTCTTC 6 cut(s) 873, 978, 1074, 1179, 1407, 1647
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 3 cut(s) 916, 1024, 1690
BspTNI GGTCTC 1 cut(s) 1801
BsrDI GCAATG 1 cut(s) 1752
BsrFI RCCGGY 2 cut(s) 66, 1021
BsrGI TGTACA 2 cut(s) 703, 1000
BsrI ACTGG 3 cut(s) 92, 1694, 1961
BssAI RCCGGY 2 cut(s) 66, 1021
BssECI CCNNGG 7 cut(s) 7, 207, 217, 273, 344, 591, 882
BssMI GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 4 cut(s) 7, 207, 273, 591
Bst2UI CCWGG 4 cut(s) 241, 574, 883, 1085
Bst4CI ACNGT 5 cut(s) 1218, 1281, 1584, 1835, 1972
Bst6I CTCTTC 7 cut(s) 60, 873, 978, 1074, 1179, 1407, 1647
BstACI GRCGYC 1 cut(s) 99
BstAUI TGTACA 2 cut(s) 703, 1000
BstBAI YACGTR 3 cut(s) 34, 895, 1408
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 5 cut(s) 230, 394, 1095, 2002, 2083
BstDEI CTNAG 1 cut(s) 1331
BstF5I GGATG 7 cut(s) 227, 291, 307, 517, 1678, 1863, 1885
BstFNI CGCG 2 cut(s) 173, 450
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 6 cut(s) 180, 196, 371, 505, 556, 1375
BstMAI GTCTC 5 cut(s) 79, 1123, 1374, 1801, 1878
BstMBI GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 4 cut(s) 602, 668, 677, 1364
BstNI CCWGG 4 cut(s) 241, 574, 883, 1085
BstNSI RCATGY 3 cut(s) 902, 1001, 1775
BstPAI GACNNNNGTC 1 cut(s) 9
BstSCI CCNGG 6 cut(s) 239, 343, 572, 881, 1083, 1415
BstSFI CTRYAG 2 cut(s) 75, 1658
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 2 cut(s) 173, 450
BstV1I GCAGC 4 cut(s) 890, 995, 1424, 1614
BstV2I GAAGAC 2 cut(s) 100, 1143
BstX2I RGATCY 2 cut(s) 502, 1372
BstXI CCANNNNNNTGG 4 cut(s) 247, 262, 1294, 1573
BstYI RGATCY 2 cut(s) 502, 1372
Bsu15I ATCGAT 1 cut(s) 1802
BsuRI GGCC 6 cut(s) 70, 251, 278, 572, 1097, 1565
BsuTUI ATCGAT 1 cut(s) 1802
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 7 cut(s) 227, 291, 307, 517, 1678, 1863, 1885
BtsI GCAGTG 3 cut(s) 338, 778, 829
BtsIMutI CAGTG 4 cut(s) 338, 778, 829, 1546
BveI ACCTGC 4 cut(s) 25, 82, 159, 544
Cac8I GCNNGC 5 cut(s) 230, 394, 1095, 2002, 2083
CciI TCATGA 1 cut(s) 924
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 66, 1021
Cfr13I GGNCC 7 cut(s) 4, 11, 211, 576, 1563, 1632, 2042
ClaI ATCGAT 1 cut(s) 1802
CseI GACGC 2 cut(s) 88, 1736
Csp6I GTAC 5 cut(s) 244, 704, 1001, 1585, 1655
CviQI GTAC 5 cut(s) 244, 704, 1001, 1585, 1655
DdeI CTNAG 1 cut(s) 1331
DpnI GATC 6 cut(s) 179, 195, 370, 504, 555, 1374
DpnII GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
DraI TTTAAA 1 cut(s) 943
DraIII CACNNNGTG 1 cut(s) 34
DrdI GACNNNNNNGTC 1 cut(s) 134
DriI GACNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 2 cut(s) 68, 249
Eam1104I CTCTTC 7 cut(s) 60, 873, 978, 1074, 1179, 1407, 1647
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 7 cut(s) 60, 873, 978, 1074, 1179, 1407, 1647
EciI GGCGGA 1 cut(s) 203
Eco130I CCWWGG 4 cut(s) 7, 207, 273, 591
Eco147I AGGCCT 1 cut(s) 572
Eco31I GGTCTC 1 cut(s) 1801
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 6 cut(s) 4, 11, 211, 576, 1632, 2042
Eco57I CTGAAG 3 cut(s) 519, 1518, 1647
Eco72I CACGTG 1 cut(s) 895
Eco88I CYCGRG 1 cut(s) 2036
EcoO109I RGGNCCY 1 cut(s) 2042
EcoRI GAATTC 1 cut(s) 1547
EcoRII CCWGG 4 cut(s) 239, 572, 881, 1083
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 4 cut(s) 7, 207, 273, 591
ErhI CCWWGG 4 cut(s) 7, 207, 273, 591
Esp3I CGTCTC 1 cut(s) 1374
FalI AAGNNNNNCTT 2 cut(s) 1247, 1279
FaqI GGGAC 2 cut(s) 361, 2055
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 5 cut(s) 359, 879, 984, 1413, 1603
FokI GGATG 7 cut(s) 234, 278, 294, 524, 1685, 1870, 1892
Fsp4HI GCNGC 5 cut(s) 359, 879, 984, 1413, 1603
FspBI CTAG 9 cut(s) 393, 545, 626, 980, 1181, 1340, 1440, 1649, 2082
GlaI GCGC 1 cut(s) 271
GluI GCNGC 5 cut(s) 359, 879, 984, 1413, 1603
GsuI CTGGAG 1 cut(s) 223
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 6 cut(s) 70, 251, 278, 572, 1097, 1565
HapII CCGG 6 cut(s) 67, 311, 344, 362, 1022, 1417
HgaI GACGC 2 cut(s) 88, 1736
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 99
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 4 cut(s) 44, 236, 656, 761
HpaII CCGG 6 cut(s) 67, 311, 344, 362, 1022, 1417
HphI GGTGA 8 cut(s) 385, 788, 1070, 1169, 1250, 1316, 1637, 1997
Hpy166II GTNNAC 4 cut(s) 14, 1284, 1635, 2057
Hpy188I TCNGA 4 cut(s) 140, 193, 221, 499
Hpy188III TCNNGA 4 cut(s) 197, 434, 925, 2036
Hpy8I GTNNAC 4 cut(s) 14, 1284, 1635, 2057
Hpy99I CGWCG 4 cut(s) 104, 284, 320, 355
HpyAV CCTTC 5 cut(s) 881, 985, 1082, 1622, 1703
HpyCH4III ACNGT 5 cut(s) 1218, 1281, 1584, 1835, 1972
HpyCH4IV ACGT 6 cut(s) 33, 353, 894, 1200, 1407, 1434
HpyF10VI GCNNNNNNNGC 4 cut(s) 602, 668, 677, 1364
HpyF3I CTNAG 1 cut(s) 1331
HpySE526I ACGT 6 cut(s) 33, 353, 894, 1200, 1407, 1434
Hsp92I GRCGYC 1 cut(s) 99
HspAI GCGC 1 cut(s) 270
KflI GGGWCCC 1 cut(s) 2042
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
LguI GCTCTTC 6 cut(s) 873, 978, 1074, 1179, 1407, 1647
LmnI GCTCC 3 cut(s) 137, 163, 225
Lsp1109I GCAGC 4 cut(s) 890, 995, 1424, 1614
LweI GCATC 1 cut(s) 1147
MaeI CTAG 9 cut(s) 393, 545, 626, 980, 1181, 1340, 1440, 1649, 2082
MaeII ACGT 6 cut(s) 33, 353, 894, 1200, 1407, 1434
MaeIII GTNAC 9 cut(s) 471, 962, 1058, 1163, 1222, 1435, 1455, 1966, 2017
MalI GATC 6 cut(s) 179, 195, 370, 504, 555, 1374
MboI GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
MflI RGATCY 2 cut(s) 502, 1372
MhlI GDGCHC 1 cut(s) 894
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 5 cut(s) 606, 613, 1146, 1484, 1547
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 230, 770
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 251
MseI TTAA 8 cut(s) 62, 444, 942, 1149, 1749, 1853, 1866, 2116
MslI CAYNNNNRTG 3 cut(s) 260, 897, 1292
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 125, 1496
MspI CCGG 6 cut(s) 67, 311, 344, 362, 1022, 1417
MspR9I CCNGG 6 cut(s) 241, 345, 574, 883, 1085, 1417
Mva1269I GAATGC 2 cut(s) 166, 592
MvaI CCWGG 4 cut(s) 241, 574, 883, 1085
MvnI CGCG 2 cut(s) 173, 450
MwoI GCNNNNNNNGC 4 cut(s) 602, 668, 677, 1364
NciI CCSGG 2 cut(s) 345, 1417
NdeI CATATG 1 cut(s) 853
NdeII GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
NheI GCTAGC 2 cut(s) 392, 2081
NlaIV GGNNCC 8 cut(s) 133, 534, 918, 1026, 1633, 1692, 2043, 2044
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 4 cut(s) 962, 1058, 1966, 2017
NspI RCATGY 3 cut(s) 902, 1001, 1775
NspV TTCGAA 1 cut(s) 47
OliI CACNNNNGTG 1 cut(s) 1292
PaeR7I CTCGAG 1 cut(s) 2036
PagI TCATGA 1 cut(s) 924
PaqCI CACCTGC 2 cut(s) 25, 82
PceI AGGCCT 1 cut(s) 572
PciSI GCTCTTC 6 cut(s) 873, 978, 1074, 1179, 1407, 1647
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 189, 377
PctI GAATGC 2 cut(s) 166, 592
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 44, 656
PfoI TCCNGGA 1 cut(s) 1083
PkrI GCNGC 5 cut(s) 360, 880, 985, 1414, 1604
PleI GAGTC 2 cut(s) 230, 769
PmaCI CACGTG 1 cut(s) 895
PmlI CACGTG 1 cut(s) 895
PpsI GAGTC 2 cut(s) 230, 769
Ppu21I YACGTR 3 cut(s) 34, 895, 1408
PpuMI RGGWCCY 1 cut(s) 2042
PshAI GACNNNNGTC 1 cut(s) 9
PsiI TTATAA 2 cut(s) 567, 644
Psp5II RGGWCCY 1 cut(s) 2042
Psp6I CCWGG 4 cut(s) 239, 572, 881, 1083
PspCI CACGTG 1 cut(s) 895
PspGI CCWGG 4 cut(s) 239, 572, 881, 1083
PspN4I GGNNCC 8 cut(s) 133, 534, 918, 1026, 1633, 1692, 2043, 2044
PspPI GGNCC 7 cut(s) 4, 11, 211, 576, 1563, 1632, 2042
PspPPI RGGWCCY 1 cut(s) 2042
PstI CTGCAG 2 cut(s) 79, 1662
PsuI RGATCY 2 cut(s) 502, 1372
RsaI GTAC 5 cut(s) 245, 705, 1002, 1586, 1656
RsaNI GTAC 5 cut(s) 244, 704, 1001, 1585, 1655
RseI CAYNNNNRTG 3 cut(s) 260, 897, 1292
SapI GCTCTTC 6 cut(s) 873, 978, 1074, 1179, 1407, 1647
SaqAI TTAA 8 cut(s) 62, 444, 942, 1149, 1749, 1853, 1866, 2116
SatI GCNGC 5 cut(s) 359, 879, 984, 1413, 1603
Sau3AI GATC 6 cut(s) 177, 193, 368, 502, 553, 1372
Sau96I GGNCC 7 cut(s) 4, 11, 211, 576, 1563, 1632, 2042
ScaI AGTACT 1 cut(s) 1656
SchI GAGTC 2 cut(s) 230, 770
ScrFI CCNGG 6 cut(s) 241, 345, 574, 883, 1085, 1417
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 1 cut(s) 1147
SfcI CTRYAG 2 cut(s) 75, 1658
Sfr274I CTCGAG 1 cut(s) 2036
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 6 cut(s) 4, 11, 211, 576, 1632, 2042
SlaI CTCGAG 1 cut(s) 2036
SmiMI CAYNNNNRTG 3 cut(s) 260, 897, 1292
SmlI CTYRAG 1 cut(s) 2036
SmoI CTYRAG 1 cut(s) 2036
Sse9I AATT 5 cut(s) 606, 613, 1146, 1484, 1547
SseBI AGGCCT 1 cut(s) 572
SspI AATATT 2 cut(s) 1249, 1871
SspMI CTAG 9 cut(s) 393, 545, 626, 980, 1181, 1340, 1440, 1649, 2082
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 6 cut(s) 239, 343, 572, 881, 1083, 1415
StyI CCWWGG 4 cut(s) 7, 207, 273, 591
TaaI ACNGT 5 cut(s) 1218, 1281, 1584, 1835, 1972
TaiI ACGT 6 cut(s) 36, 356, 897, 1203, 1410, 1437
TaqI TCGA 8 cut(s) 47, 147, 288, 318, 519, 1802, 1875, 2037
TasI AATT 5 cut(s) 606, 613, 1146, 1484, 1547
TatI WGTACW 3 cut(s) 703, 1000, 1654
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 2 cut(s) 44, 656
Tru1I TTAA 8 cut(s) 62, 444, 942, 1149, 1749, 1853, 1866, 2116
Tru9I TTAA 8 cut(s) 62, 444, 942, 1149, 1749, 1853, 1866, 2116
TscAI CASTG 4 cut(s) 345, 778, 829, 1546
TseFI GTSAC 4 cut(s) 962, 1058, 1966, 2017
TseI GCWGC 4 cut(s) 878, 983, 1412, 1602
Tsp45I GTSAC 4 cut(s) 962, 1058, 1966, 2017
TspDTI ATGAA 6 cut(s) 606, 745, 860, 913, 965, 1061
TspGWI ACGGA 2 cut(s) 124, 545
TspRI CASTG 4 cut(s) 345, 778, 829, 1546
VpaK11BI GGWCC 6 cut(s) 4, 11, 211, 576, 1632, 2042
XapI RAATTY 2 cut(s) 613, 1547
XceI RCATGY 3 cut(s) 902, 1001, 1775
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 2036
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 9 cut(s) 393, 545, 626, 980, 1181, 1340, 1440, 1649, 2082
ZrmI AGTACT 1 cut(s) 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.